AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0099600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0099600 MtrunA17_Chr1g0150821 66.667 156 35 2 2 153 121 263 2.06e-58 182
Msa0099600 MtrunA17_Chr8g0343441 64.706 153 37 3 2 153 120 256 1.18e-49 159
Msa0099600 MtrunA17_Chr2g0300241 56.494 154 42 3 2 153 123 253 6.50e-46 150
Msa0099600 MtrunA17_Chr2g0329821 61.184 152 41 3 2 152 116 250 5.80e-42 140
Msa0099600 MtrunA17_Chr4g0076771 50.658 152 56 4 2 152 121 254 2.09e-39 133
Msa0099600 MtrunA17_Chr4g0006381 47.712 153 53 5 1 153 117 242 7.56e-33 116
Msa0099600 MtrunA17_Chr7g0242511 43.791 153 59 5 2 153 116 242 3.80e-30 109
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0099600 AT5G20510.2 57.516 153 51 2 2 153 80 219 3.14e-49 157
Msa0099600 AT3G42790.1 56.954 151 43 3 2 152 121 249 3.55e-49 159
Msa0099600 AT5G20510.1 57.516 153 51 2 2 153 121 260 8.89e-49 158
Msa0099600 AT5G26210.1 58.940 151 46 2 2 152 120 254 7.41e-41 137
Msa0099600 AT2G02470.1 50.658 152 60 4 2 153 120 256 6.00e-39 132
Msa0099600 AT2G02470.2 50.658 152 51 4 2 153 120 247 6.13e-39 132
Msa0099600 AT5G05610.2 44.737 152 55 4 2 152 117 240 2.10e-33 118
Msa0099600 AT5G05610.1 44.737 152 55 4 2 152 117 240 2.10e-33 118
Msa0099600 AT3G11200.1 44.371 151 60 4 2 152 119 245 4.27e-33 117
Msa0099600 AT3G11200.2 44.371 151 60 4 2 152 106 232 5.48e-33 117
Msa0099600 AT1G14510.1 48.993 149 56 4 2 150 120 248 1.16e-32 116

Find 32 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTGCATTGCCATTGATAT+TGG 0.306155 1_3:-5812765 None:intergenic
CTTAGAGTTTGATTTGGATT+TGG 0.307586 1_3:-5812151 None:intergenic
GGCAATGCAAATGAAGTTAA+AGG 0.322830 1_3:+5812776 Msa0099600:CDS
GCTATGCAGCTGATGAGTTC+TGG 0.348702 1_3:+5812874 Msa0099600:CDS
ACAATCTTTGAAGCTGTTGC+AGG 0.355548 1_3:+5812075 Msa0099600:CDS
ATTTGTGAGAAATCGTTTCA+TGG 0.364470 1_3:+5812909 Msa0099600:CDS
TTGATATGCTCAGCCCTTGC+CGG 0.375496 1_3:-5812951 None:intergenic
TCTTACCTTAGAGTTTGATT+TGG 0.384520 1_3:-5812157 None:intergenic
TTTGAAGCTGTTGCAGGGTC+TGG 0.385521 1_3:+5812081 Msa0099600:CDS
CAAATCCAAATCAAACTCTA+AGG 0.411797 1_3:+5812152 Msa0099600:CDS
GCAACAGCTTCAAAGATTGT+TGG 0.414898 1_3:-5812072 None:intergenic
GTTAAAGGACGAGGAGGTTG+AGG 0.415516 1_3:+5812791 Msa0099600:CDS
TAGCAAATTATTAACATCAA+TGG 0.454579 1_3:-5812207 None:intergenic
TTATTAACATCAATGGATAG+TGG 0.466987 1_3:-5812200 None:intergenic
GCTCTCTTGTTGCTGCAAGC+TGG 0.467608 1_3:-5812987 None:intergenic
CGTGAAGATTACACCGGCAA+GGG 0.539345 1_3:+5812938 Msa0099600:CDS
GCAGGGTCTGGTAGCAAACA+AGG 0.540889 1_3:+5812093 Msa0099600:CDS
AAGGACGAGGAGGTTGAGGA+TGG 0.556879 1_3:+5812795 Msa0099600:CDS
CAATCTTTGAAGCTGTTGCA+GGG 0.574674 1_3:+5812076 Msa0099600:CDS
GTCTGGTAGCAAACAAGGAA+AGG 0.575473 1_3:+5812098 Msa0099600:CDS
GGTTGAGGATGGTGTAGGTG+TGG 0.578977 1_3:+5812806 Msa0099600:CDS
GAGGAGGTTGAGGATGGTGT+AGG 0.579309 1_3:+5812801 Msa0099600:CDS
GCGTGAAGATTACACCGGCA+AGG 0.599177 1_3:+5812937 Msa0099600:CDS
GAGACTTTGTGTGGGGCATG+TGG 0.613065 1_3:+5812846 Msa0099600:CDS
GCAAATGAAGTTAAAGGACG+AGG 0.616669 1_3:+5812782 Msa0099600:CDS
AACACGGAGAGACTTTGTGT+GGG 0.622939 1_3:+5812838 Msa0099600:CDS
GGATGGTGTAGGTGTGGACG+AGG 0.628142 1_3:+5812812 Msa0099600:CDS
GAACACGGAGAGACTTTGTG+TGG 0.630911 1_3:+5812837 Msa0099600:CDS
ACACGGAGAGACTTTGTGTG+GGG 0.707279 1_3:+5812839 Msa0099600:CDS
AATGAAGTTAAAGGACGAGG+AGG 0.713570 1_3:+5812785 Msa0099600:CDS
GGTGTGGACGAGGAAGAACA+CGG 0.729144 1_3:+5812822 Msa0099600:CDS
AAAATGCGTGAAGATTACAC+CGG 0.768073 1_3:+5812932 Msa0099600:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TACATTACATTTTTTTTAAT+TGG - chr1_3:5808994-5809013 None:intergenic 10.0%
!!! AAATGTAATGTAATTTTTTT+AGG + chr1_3:5809002-5809021 Msa0099600:intron 10.0%
!!! TCATATATTAATGTTTTATA+CGG - chr1_3:5809098-5809117 None:intergenic 10.0%
!! ATTAATATATGAATAATGTA+TGG + chr1_3:5809106-5809125 Msa0099600:intron 10.0%
!! AGTACATATTTAATTATTAA+GGG - chr1_3:5809419-5809438 None:intergenic 10.0%
!! TAGTACATATTTAATTATTA+AGG - chr1_3:5809420-5809439 None:intergenic 10.0%
!!! ATGAATTTATTTTTCTTAAA+TGG - chr1_3:5809502-5809521 None:intergenic 10.0%
!! CTTTATAATTTAAATATCAA+TGG + chr1_3:5809524-5809543 Msa0099600:intron 10.0%
!! TTACTAAATACAATAAAATA+TGG - chr1_3:5809615-5809634 None:intergenic 10.0%
!!! TTGTTTCATATTTTATAATA+GGG + chr1_3:5809657-5809676 Msa0099600:intron 10.0%
!! TTACAAAAAAAATAAGATAA+AGG - chr1_3:5810901-5810920 None:intergenic 10.0%
!!! TTATTTTTTTTGTAACAATT+TGG + chr1_3:5810906-5810925 Msa0099600:intron 10.0%
!! TTAATAAAAAAATGAGAAAA+AGG + chr1_3:5811473-5811492 Msa0099600:intron 10.0%
!!! TTAATTTTTCATAACTAGTA+AGG + chr1_3:5809043-5809062 Msa0099600:intron 15.0%
!!! ATGTATATTTTAACATAAGA+GGG - chr1_3:5809331-5809350 None:intergenic 15.0%
!!! AATGTATATTTTAACATAAG+AGG - chr1_3:5809332-5809351 None:intergenic 15.0%
!!! AGATTTTATTTTTAAAAGGA+GGG - chr1_3:5809356-5809375 None:intergenic 15.0%
!!! TAGATTTTATTTTTAAAAGG+AGG - chr1_3:5809357-5809376 None:intergenic 15.0%
!!! GTGTAGATTTTATTTTTAAA+AGG - chr1_3:5809360-5809379 None:intergenic 15.0%
!!! AATTTATTTTTCTTAAATGG+TGG - chr1_3:5809499-5809518 None:intergenic 15.0%
!!! GTTGTTTCATATTTTATAAT+AGG + chr1_3:5809656-5809675 Msa0099600:intron 15.0%
!!! AAGAAATTTTATGAATGTAA+CGG + chr1_3:5810747-5810766 Msa0099600:intron 15.0%
!! TGAACAACTAAATATAATTA+AGG + chr1_3:5810857-5810876 Msa0099600:intron 15.0%
!! TAACATTAATTCATGATATT+TGG + chr1_3:5811340-5811359 Msa0099600:intron 15.0%
!! GTTACAAATAAATAAGATAA+AGG + chr1_3:5811563-5811582 Msa0099600:intron 15.0%
!!! TATGCATATTACATTTTGAT+TGG - chr1_3:5809189-5809208 None:intergenic 20.0%
!! AAAAGTTCATCTTAATCATA+AGG + chr1_3:5809256-5809275 Msa0099600:intron 20.0%
!!! TGTATATTTTAACATAAGAG+GGG - chr1_3:5809330-5809349 None:intergenic 20.0%
!! TATTTAATTATTAAGGGAGA+GGG - chr1_3:5809413-5809432 None:intergenic 20.0%
!! ATATTTAATTATTAAGGGAG+AGG - chr1_3:5809414-5809433 None:intergenic 20.0%
!!! TTATTTTTGCTAGATTATTG+AGG + chr1_3:5809799-5809818 Msa0099600:intron 20.0%
!!! GAAATGTTTTCTAATATGTT+TGG + chr1_3:5809892-5809911 Msa0099600:intron 20.0%
!!! AGTGTATATTTTAACATAAC+AGG + chr1_3:5809931-5809950 Msa0099600:intron 20.0%
!!! GTGTATATTTTAACATAACA+GGG + chr1_3:5809932-5809951 Msa0099600:intron 20.0%
!!! TGTATATTTTAACATAACAG+GGG + chr1_3:5809933-5809952 Msa0099600:intron 20.0%
!! TAAAGAAGAAATTTGAATGA+AGG - chr1_3:5810180-5810199 None:intergenic 20.0%
!! CAAAATTGAACAATCAAAAT+GGG - chr1_3:5810721-5810740 None:intergenic 20.0%
!! ACAAAATTGAACAATCAAAA+TGG - chr1_3:5810720-5810739 None:intergenic 20.0%
!!! CTTTTTGATTTGATTTTTCT+TGG - chr1_3:5810581-5810600 None:intergenic 20.0%
!! ACAAAATTGAACAATCAAAA+TGG + chr1_3:5810720-5810739 Msa0099600:intron 20.0%
!! CAAAATTGAACAATCAAAAT+GGG + chr1_3:5810721-5810740 Msa0099600:intron 20.0%
!!! ATCTTTATTTTTTTCCGTTA+AGG - chr1_3:5811530-5811549 None:intergenic 20.0%
!! ACGGAAAAAAATAAAGATAA+AGG + chr1_3:5811532-5811551 Msa0099600:intron 20.0%
!! CTTATTTATTTGTAACACTT+TGG - chr1_3:5811559-5811578 None:intergenic 20.0%
!! TATAGACAAGATTACATTAT+AGG - chr1_3:5811590-5811609 None:intergenic 20.0%
!! AATCAAAATGTAGTATACAT+TGG - chr1_3:5811705-5811724 None:intergenic 20.0%
!!! AATGTATACTACATTTTGAT+TGG + chr1_3:5811704-5811723 Msa0099600:intron 20.0%
!! TAGCAAATTATTAACATCAA+TGG - chr1_3:5812210-5812229 None:intergenic 20.0%
!!! ATTGATGTTAATAATTTGCT+AGG + chr1_3:5812209-5812228 Msa0099600:intron 20.0%
!!! TGTAATTTTTTTAGGCAAAG+TGG + chr1_3:5809010-5809029 Msa0099600:intron 25.0%
!! TCCAGTAGGTTAATTTTATT+TGG - chr1_3:5809069-5809088 None:intergenic 25.0%
!! GATTGGTTAATATTATGAGA+GGG - chr1_3:5809172-5809191 None:intergenic 25.0%
!! TGATTGGTTAATATTATGAG+AGG - chr1_3:5809173-5809192 None:intergenic 25.0%
! TGTAATTCAAGCTTCTATTA+AGG + chr1_3:5809860-5809879 Msa0099600:intron 25.0%
! GTAATTCAAGCTTCTATTAA+GGG + chr1_3:5809861-5809880 Msa0099600:intron 25.0%
!! TCTAATATGTTTGGAATAAG+AGG + chr1_3:5809901-5809920 Msa0099600:intron 25.0%
!! CTAATATGTTTGGAATAAGA+GGG + chr1_3:5809902-5809921 Msa0099600:intron 25.0%
!! TAATATGTTTGGAATAAGAG+GGG + chr1_3:5809903-5809922 Msa0099600:intron 25.0%
! TGTAATTCAAGAATCTTTGA+GGG + chr1_3:5809963-5809982 Msa0099600:intron 25.0%
! TACATCAAATTTCTTTCATC+TGG + chr1_3:5810078-5810097 Msa0099600:intron 25.0%
! GAATGTAAGTGATGAATTTA+AGG - chr1_3:5810108-5810127 None:intergenic 25.0%
! GAAACAAGAATTGATCAATA+GGG - chr1_3:5810208-5810227 None:intergenic 25.0%
! AGAAACAAGAATTGATCAAT+AGG - chr1_3:5810209-5810228 None:intergenic 25.0%
! AAGTCATCTGTTATACTTTA+AGG - chr1_3:5810528-5810547 None:intergenic 25.0%
!!! GAATGTAACGGATAAATTTA+AGG + chr1_3:5810759-5810778 Msa0099600:intron 25.0%
! AATTAAGGCAAGATTACATT+TGG + chr1_3:5810872-5810891 Msa0099600:intron 25.0%
! ATTAAGGCAAGATTACATTT+GGG + chr1_3:5810873-5810892 Msa0099600:intron 25.0%
!!! ATCTTTATTTTTTCCCGTTT+AGG + chr1_3:5810935-5810954 Msa0099600:intron 25.0%
! AAAAATTCAGAAATGTTGCA+AGG + chr1_3:5811054-5811073 Msa0099600:intron 25.0%
! TACAAAAGTTAAGATTGTTC+CGG + chr1_3:5811118-5811137 Msa0099600:intron 25.0%
!!! TTTGGGTTTATTTTTATGCT+GGG + chr1_3:5811176-5811195 Msa0099600:intron 25.0%
!! ATCTCTTTTCTATAGAGTTT+TGG + chr1_3:5811284-5811303 Msa0099600:intron 25.0%
! TGTGTTTAAAAGAAGATTTG+AGG + chr1_3:5811311-5811330 Msa0099600:intron 25.0%
! TGAGATATAAAAGACCTTAA+CGG + chr1_3:5811513-5811532 Msa0099600:intron 25.0%
!!! TTTTGGTTTTTCTGTATGAA+AGG + chr1_3:5811817-5811836 Msa0099600:intron 25.0%
! TTATTAACATCAATGGATAG+TGG - chr1_3:5812203-5812222 None:intergenic 25.0%
! GTCATTTAATCTTGATTGAA+AGG - chr1_3:5812369-5812388 None:intergenic 25.0%
! TTTATAGTTCAAGAACGTAT+CGG + chr1_3:5812574-5812593 Msa0099600:intron 25.0%
! GTTTGGTATTTAATCAGTTA+AGG + chr1_3:5812680-5812699 Msa0099600:intron 25.0%
!!! GATATTGGTTTTGAAAACTT+TGG - chr1_3:5812753-5812772 None:intergenic 25.0%
!! GTTTTCAAAACCAATATCAA+TGG + chr1_3:5812755-5812774 Msa0099600:CDS 25.0%
GCCAAATAAAATTAACCTAC+TGG + chr1_3:5809065-5809084 Msa0099600:intron 30.0%
!!! TATTTTAACATAAGAGGGGA+GGG - chr1_3:5809326-5809345 None:intergenic 30.0%
!!! ATATTTTAACATAAGAGGGG+AGG - chr1_3:5809327-5809346 None:intergenic 30.0%
TTGTAATTCAAGCATCTTAG+AGG - chr1_3:5809390-5809409 None:intergenic 30.0%
!! ACACTTAAAAAAAGTACTGC+GGG - chr1_3:5809763-5809782 None:intergenic 30.0%
!! CACACTTAAAAAAAGTACTG+CGG - chr1_3:5809764-5809783 None:intergenic 30.0%
!!! ATATTTTAACATAACAGGGG+AGG + chr1_3:5809936-5809955 Msa0099600:intron 30.0%
!!! TATTTTAACATAACAGGGGA+GGG + chr1_3:5809937-5809956 Msa0099600:intron 30.0%
GTGTAATTCAAGAATCTTTG+AGG + chr1_3:5809962-5809981 Msa0099600:intron 30.0%
GTAATTCAAGAATCTTTGAG+GGG + chr1_3:5809964-5809983 Msa0099600:intron 30.0%
!! ATGTTTTTGTTCTCACGTTT+GGG - chr1_3:5810155-5810174 None:intergenic 30.0%
! AATTTGAAACTGTAGCAGTT+TGG - chr1_3:5810273-5810292 None:intergenic 30.0%
! GTTAGCAGATTTTATTAGGT+TGG - chr1_3:5810361-5810380 None:intergenic 30.0%
!! GTTCTTTTGTTTGGACTTAA+AGG - chr1_3:5810415-5810434 None:intergenic 30.0%
!!! GAGTTTGCTAGCATTTTTTT+CGG - chr1_3:5810471-5810490 None:intergenic 30.0%
!! TTTGATTTTTCTTGGTTGTG+AGG - chr1_3:5810573-5810592 None:intergenic 30.0%
CTCACGAAAATTGAAAAATG+CGG + chr1_3:5810685-5810704 Msa0099600:intron 30.0%
TTAAGGCAAGATTACATTTG+GGG + chr1_3:5810874-5810893 Msa0099600:intron 30.0%
!! TGTGCTTTTAAGAGAGATAA+AGG - chr1_3:5810962-5810981 None:intergenic 30.0%
!!! CACATCAATTTTGTTGTTTC+CGG - chr1_3:5811140-5811159 None:intergenic 30.0%
!!! CTTTGGGTTTATTTTTATGC+TGG + chr1_3:5811175-5811194 Msa0099600:intron 30.0%
GAAGATTCAAAGAAAGAAGA+TGG + chr1_3:5811406-5811425 Msa0099600:intron 30.0%
GAACATCTTCATCATATTTC+TGG + chr1_3:5811436-5811455 Msa0099600:intron 30.0%
! TTTGATTGGTTGATATCATG+AGG + chr1_3:5811718-5811737 Msa0099600:intron 30.0%
! TTGATTGGTTGATATCATGA+GGG + chr1_3:5811719-5811738 Msa0099600:intron 30.0%
AAATACCCTCTAAATAAGAG+AGG + chr1_3:5811745-5811764 Msa0099600:intron 30.0%
AATACCCTCTAAATAAGAGA+GGG + chr1_3:5811746-5811765 Msa0099600:intron 30.0%
! ATTACCCTCTCTTATTTAGA+GGG - chr1_3:5811753-5811772 None:intergenic 30.0%
CTGAAAGGAAAGAAACAATA+AGG - chr1_3:5811876-5811895 None:intergenic 30.0%
GTTTCTTTCCTTTCAGTAAA+AGG + chr1_3:5811880-5811899 Msa0099600:intron 30.0%
! AAGATACTCCTTTTACTGAA+AGG - chr1_3:5811891-5811910 None:intergenic 30.0%
TCTTGCTAATACAGAGAATT+AGG - chr1_3:5811919-5811938 None:intergenic 30.0%
!! AACAAACTTTTGAGAGGAAA+GGG - chr1_3:5811948-5811967 None:intergenic 30.0%
!!! ATTTTTTCGCCATTCATTGA+TGG + chr1_3:5811986-5812005 Msa0099600:intron 30.0%
!! CTTAGAGTTTGATTTGGATT+TGG - chr1_3:5812154-5812173 None:intergenic 30.0%
CAAATCCAAATCAAACTCTA+AGG + chr1_3:5812152-5812171 Msa0099600:CDS 30.0%
TCTTACCTTAGAGTTTGATT+TGG - chr1_3:5812160-5812179 None:intergenic 30.0%
! AGTCGCATAATATGTTGTTT+AGG + chr1_3:5812244-5812263 Msa0099600:intron 30.0%
!!! CGAAAGTGAAAAGTATTTTG+AGG + chr1_3:5812285-5812304 Msa0099600:intron 30.0%
! CAAATCTGAAGTCATAATGT+GGG - chr1_3:5812391-5812410 None:intergenic 30.0%
! TCAAATCTGAAGTCATAATG+TGG - chr1_3:5812392-5812411 None:intergenic 30.0%
GATTAAATACCAAACCAATC+AGG - chr1_3:5812675-5812694 None:intergenic 30.0%
ATTTGCATTGCCATTGATAT+TGG - chr1_3:5812768-5812787 None:intergenic 30.0%
ATTTGTGAGAAATCGTTTCA+TGG + chr1_3:5812909-5812928 Msa0099600:CDS 30.0%
TTCTTGCTGAATTGAGTAGA+TGG + chr1_3:5808803-5808822 Msa0099600:intron 35.0%
CTGAATTGAGTAGATGGATA+CGG + chr1_3:5808809-5808828 Msa0099600:intron 35.0%
TTATACGGTTCATATCCAGT+AGG - chr1_3:5809083-5809102 None:intergenic 35.0%
!! ATTTTAACATAAGAGGGGAG+GGG - chr1_3:5809325-5809344 None:intergenic 35.0%
ATTCAAGCTTCTATTAAGGG+AGG + chr1_3:5809864-5809883 Msa0099600:intron 35.0%
TTCAAGCTTCTATTAAGGGA+GGG + chr1_3:5809865-5809884 Msa0099600:intron 35.0%
!! ATTTTAACATAACAGGGGAG+GGG + chr1_3:5809938-5809957 Msa0099600:intron 35.0%
!! TGTTTTTGTTCTCACGTTTG+GGG - chr1_3:5810154-5810173 None:intergenic 35.0%
!! GATGTTTTTGTTCTCACGTT+TGG - chr1_3:5810156-5810175 None:intergenic 35.0%
! GCGTGTTAGCAGATTTTATT+AGG - chr1_3:5810365-5810384 None:intergenic 35.0%
TTGTTTGGACTTAAAGGCTT+TGG - chr1_3:5810409-5810428 None:intergenic 35.0%
! GAGGCTTTTGTTCTTTTGTT+TGG - chr1_3:5810424-5810443 None:intergenic 35.0%
GTTGAAATGGTTATGTCATG+AGG - chr1_3:5810443-5810462 None:intergenic 35.0%
!! TTTTTCGGTTGCTGTTGAAA+TGG - chr1_3:5810456-5810475 None:intergenic 35.0%
!! TGTTGCTGTTGTGATGTTTT+GGG - chr1_3:5810493-5810512 None:intergenic 35.0%
! ATACTTTAAGGATTGCTAGC+AGG - chr1_3:5810516-5810535 None:intergenic 35.0%
!! TGACGTGGTTCTGAAAATAA+CGG + chr1_3:5810621-5810640 Msa0099600:intron 35.0%
! ATTAGTTTACACGACAAGAC+TGG - chr1_3:5810831-5810850 None:intergenic 35.0%
AGAGAGATAAAGGACCTAAA+CGG - chr1_3:5810952-5810971 None:intergenic 35.0%
AATTCAGAAATGTTGCAAGG+CGG + chr1_3:5811057-5811076 Msa0099600:intron 35.0%
! TGATTGGTTGATATCATGAG+GGG + chr1_3:5811720-5811739 Msa0099600:intron 35.0%
! GATTACCCTCTCTTATTTAG+AGG - chr1_3:5811754-5811773 None:intergenic 35.0%
GCATGTCGATGTATACATTA+TGG - chr1_3:5811786-5811805 None:intergenic 35.0%
! ACATGCTACACATAAGCTTT+TGG + chr1_3:5811800-5811819 Msa0099600:intron 35.0%
!! CAACAAACTTTTGAGAGGAA+AGG - chr1_3:5811949-5811968 None:intergenic 35.0%
CTTTCCTCTCAAAAGTTTGT+TGG + chr1_3:5811947-5811966 Msa0099600:intron 35.0%
GAAAGCAAACCATCAATGAA+TGG - chr1_3:5811998-5812017 None:intergenic 35.0%
!! CAAGATGGTAGTTAATTTGC+AGG + chr1_3:5812019-5812038 Msa0099600:intron 35.0%
CTTGACCATAAATATCCAGA+CGG - chr1_3:5812326-5812345 None:intergenic 35.0%
TTGCTCCAAAAACAAGGATT+CGG - chr1_3:5812546-5812565 None:intergenic 35.0%
ACTTTCTTGCTCCAAAAACA+AGG - chr1_3:5812552-5812571 None:intergenic 35.0%
TGACCATATATCCCTGTTTA+TGG + chr1_3:5812613-5812632 Msa0099600:intron 35.0%
! GTTAAGGCTGTTATCTCTTT+TGG + chr1_3:5812696-5812715 Msa0099600:intron 35.0%
!!! GGTTTTGAAAACTTTGGTTG+AGG - chr1_3:5812747-5812766 None:intergenic 35.0%
GGCAATGCAAATGAAGTTAA+AGG + chr1_3:5812776-5812795 Msa0099600:CDS 35.0%
AAAATGCGTGAAGATTACAC+CGG + chr1_3:5812932-5812951 Msa0099600:CDS 35.0%
GTAGATGGATACGGAAGTTA+AGG + chr1_3:5808818-5808837 Msa0099600:intron 40.0%
ATGGATACGGAAGTTAAGGT+TGG + chr1_3:5808822-5808841 Msa0099600:intron 40.0%
TGGATACGGAAGTTAAGGTT+GGG + chr1_3:5808823-5808842 Msa0099600:intron 40.0%
! TACGTTACCCTCTCTTGTTT+AGG + chr1_3:5809136-5809155 Msa0099600:intron 40.0%
! ACGTTACCCTCTCTTGTTTA+GGG + chr1_3:5809137-5809156 Msa0099600:intron 40.0%
ATTACCCCCTAAACAAGAGA+GGG - chr1_3:5809146-5809165 None:intergenic 40.0%
AATTACCCCCTAAACAAGAG+AGG - chr1_3:5809147-5809166 None:intergenic 40.0%
TTCAAGCATCTTAGAGGAAG+AGG - chr1_3:5809384-5809403 None:intergenic 40.0%
TCAAGCTTCTATTAAGGGAG+GGG + chr1_3:5809866-5809885 Msa0099600:intron 40.0%
! TATGTTTGGAATAAGAGGGG+AGG + chr1_3:5809906-5809925 Msa0099600:intron 40.0%
! ATGTTTGGAATAAGAGGGGA+GGG + chr1_3:5809907-5809926 Msa0099600:intron 40.0%
! TAAAGGCTTTGGCTGATAGT+GGG - chr1_3:5810398-5810417 None:intergenic 40.0%
! TTAAAGGCTTTGGCTGATAG+TGG - chr1_3:5810399-5810418 None:intergenic 40.0%
!! GTGTTGCTGTTGTGATGTTT+TGG - chr1_3:5810494-5810513 None:intergenic 40.0%
TTTCAGAACCACGTCAGATA+TGG - chr1_3:5810617-5810636 None:intergenic 40.0%
GAGAGATAAAGGACCTAAAC+GGG - chr1_3:5810951-5810970 None:intergenic 40.0%
! TGCAAAGCTGCTCTAAACTT+TGG + chr1_3:5811158-5811177 Msa0099600:intron 40.0%
! GCAAAGCTGCTCTAAACTTT+GGG + chr1_3:5811159-5811178 Msa0099600:intron 40.0%
! GATTGGTTGATATCATGAGG+GGG + chr1_3:5811721-5811740 Msa0099600:intron 40.0%
! ACTGCCAACAAACTTTTGAG+AGG - chr1_3:5811954-5811973 None:intergenic 40.0%
!! GATGGTTTGCTTTCTCAAGA+TGG + chr1_3:5812004-5812023 Msa0099600:intron 40.0%
GCAACAGCTTCAAAGATTGT+TGG - chr1_3:5812075-5812094 None:intergenic 40.0%
!! ACAATCTTTGAAGCTGTTGC+AGG + chr1_3:5812075-5812094 Msa0099600:CDS 40.0%
!! CAATCTTTGAAGCTGTTGCA+GGG + chr1_3:5812076-5812095 Msa0099600:CDS 40.0%
TGAAAGGTTATCAGAGAGTG+CGG - chr1_3:5812353-5812372 None:intergenic 40.0%
!! TAGTCTAGTTTGCTTGACTG+TGG + chr1_3:5812490-5812509 Msa0099600:intron 40.0%
!!! GAGATCCGAATCCTTGTTTT+TGG + chr1_3:5812538-5812557 Msa0099600:intron 40.0%
! ACTTTCTAGCGCCATAAACA+GGG - chr1_3:5812627-5812646 None:intergenic 40.0%
GAATCTGTCTGTTTCCTGAT+TGG + chr1_3:5812658-5812677 Msa0099600:intron 40.0%
! TGTCTGTTTCCTGATTGGTT+TGG + chr1_3:5812663-5812682 Msa0099600:intron 40.0%
GCAAATGAAGTTAAAGGACG+AGG + chr1_3:5812782-5812801 Msa0099600:CDS 40.0%
AATGAAGTTAAAGGACGAGG+AGG + chr1_3:5812785-5812804 Msa0099600:CDS 40.0%
! CGGAAGTTAAGGTTGGGTTT+TGG + chr1_3:5808829-5808848 Msa0099600:intron 45.0%
! CGTTACCCTCTCTTGTTTAG+GGG + chr1_3:5809138-5809157 Msa0099600:intron 45.0%
! GTTACCCTCTCTTGTTTAGG+GGG + chr1_3:5809139-5809158 Msa0099600:intron 45.0%
! TGTTTGGAATAAGAGGGGAG+GGG + chr1_3:5809908-5809927 Msa0099600:intron 45.0%
!! GTTGTTTCTAGAACTGCGTC+AGG + chr1_3:5810308-5810327 Msa0099600:intron 45.0%
TCAACTCACCATATCTGACG+TGG + chr1_3:5810606-5810625 Msa0099600:intron 45.0%
GTCTGGTAGCAAACAAGGAA+AGG + chr1_3:5812098-5812117 Msa0099600:CDS 45.0%
CATAAATATCCAGACGGCAG+GGG - chr1_3:5812320-5812339 None:intergenic 45.0%
CCATAAATATCCAGACGGCA+GGG - chr1_3:5812321-5812340 None:intergenic 45.0%
ACCATAAATATCCAGACGGC+AGG - chr1_3:5812322-5812341 None:intergenic 45.0%
ATGTGTGAATCCTTTGCTGC+TGG + chr1_3:5812516-5812535 Msa0099600:intron 45.0%
GCGCCATAAACAGGGATATA+TGG - chr1_3:5812619-5812638 None:intergenic 45.0%
! GACTTTCTAGCGCCATAAAC+AGG - chr1_3:5812628-5812647 None:intergenic 45.0%
AACACGGAGAGACTTTGTGT+GGG + chr1_3:5812838-5812857 Msa0099600:CDS 45.0%
!!! ATTATTGTAATTTTATTTTT+TGG + chr1_3:5809634-5809653 Msa0099600:intron 5.0%
!!! TCTTTTATTTAATTATATTA+AGG + chr1_3:5810004-5810023 Msa0099600:intron 5.0%
!! TAGAAAATAATAATAATATA+CGG + chr1_3:5811213-5811232 Msa0099600:intron 5.0%
!!! TAAAAAAAAAAATCAATTTT+AGG - chr1_3:5811644-5811663 None:intergenic 5.0%
! TCACGTTTGGGGTTGTGGAA+TGG - chr1_3:5810143-5810162 None:intergenic 50.0%
! TGTTCTCACGTTTGGGGTTG+TGG - chr1_3:5810148-5810167 None:intergenic 50.0%
CAGAAATGTTGCAAGGCGGA+TGG + chr1_3:5811061-5811080 Msa0099600:intron 50.0%
AAATGTTGCAAGGCGGATGG+TGG + chr1_3:5811064-5811083 Msa0099600:intron 50.0%
! TTTGAAGCTGTTGCAGGGTC+TGG + chr1_3:5812081-5812100 Msa0099600:CDS 50.0%
AATTTAGATCCCCTGCCGTC+TGG + chr1_3:5812308-5812327 Msa0099600:intron 50.0%
CCCTGCCGTCTGGATATTTA+TGG + chr1_3:5812318-5812337 Msa0099600:intron 50.0%
ATTCGGATCTCCAGCAGCAA+AGG - chr1_3:5812529-5812548 None:intergenic 50.0%
GTTAAAGGACGAGGAGGTTG+AGG + chr1_3:5812791-5812810 Msa0099600:CDS 50.0%
GAACACGGAGAGACTTTGTG+TGG + chr1_3:5812837-5812856 Msa0099600:CDS 50.0%
ACACGGAGAGACTTTGTGTG+GGG + chr1_3:5812839-5812858 Msa0099600:CDS 50.0%
GCTATGCAGCTGATGAGTTC+TGG + chr1_3:5812874-5812893 Msa0099600:CDS 50.0%
CGTGAAGATTACACCGGCAA+GGG + chr1_3:5812938-5812957 Msa0099600:CDS 50.0%
TTGATATGCTCAGCCCTTGC+CGG - chr1_3:5812954-5812973 None:intergenic 50.0%
!! GCTTTGGCTGATAGTGGGAC+AGG - chr1_3:5810393-5810412 None:intergenic 55.0%
!! GATGGTGGAGGCTTAGTCAC+TGG + chr1_3:5811079-5811098 Msa0099600:intron 55.0%
AGGCTTAGTCACTGGCGGAA+GGG + chr1_3:5811087-5811106 Msa0099600:intron 55.0%
TAGTCACTGGCGGAAGGGTT+AGG + chr1_3:5811092-5811111 Msa0099600:intron 55.0%
GCAGGGTCTGGTAGCAAACA+AGG + chr1_3:5812093-5812112 Msa0099600:CDS 55.0%
AAGGACGAGGAGGTTGAGGA+TGG + chr1_3:5812795-5812814 Msa0099600:CDS 55.0%
GAGGAGGTTGAGGATGGTGT+AGG + chr1_3:5812801-5812820 Msa0099600:CDS 55.0%
GGTTGAGGATGGTGTAGGTG+TGG + chr1_3:5812806-5812825 Msa0099600:CDS 55.0%
GGTGTGGACGAGGAAGAACA+CGG + chr1_3:5812822-5812841 Msa0099600:CDS 55.0%
GAGACTTTGTGTGGGGCATG+TGG + chr1_3:5812846-5812865 Msa0099600:CDS 55.0%
GCGTGAAGATTACACCGGCA+AGG + chr1_3:5812937-5812956 Msa0099600:CDS 55.0%
GCTCTCTTGTTGCTGCAAGC+TGG - chr1_3:5812990-5813009 None:intergenic 55.0%
! TGTTGCAAGGCGGATGGTGG+AGG + chr1_3:5811067-5811086 Msa0099600:intron 60.0%
GGTGGAGGCTTAGTCACTGG+CGG + chr1_3:5811082-5811101 Msa0099600:intron 60.0%
GAGGCTTAGTCACTGGCGGA+AGG + chr1_3:5811086-5811105 Msa0099600:intron 60.0%
GGATGGTGTAGGTGTGGACG+AGG + chr1_3:5812812-5812831 Msa0099600:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 5808758 5813019 5808758 ID=Msa0099600;Name=Msa0099600
chr1_3 mRNA 5808758 5813019 5808758 ID=Msa0099600-mRNA-1;Parent=Msa0099600;Name=Msa0099600-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0|0.66|0.5|0.33|3|0|153
chr1_3 exon 5808758 5808762 5808758 ID=Msa0099600-mRNA-1:exon:2224;Parent=Msa0099600-mRNA-1
chr1_3 exon 5812041 5812214 5812041 ID=Msa0099600-mRNA-1:exon:2225;Parent=Msa0099600-mRNA-1
chr1_3 exon 5812737 5813019 5812737 ID=Msa0099600-mRNA-1:exon:2226;Parent=Msa0099600-mRNA-1
chr1_3 CDS 5808758 5808762 5808758 ID=Msa0099600-mRNA-1:cds;Parent=Msa0099600-mRNA-1
chr1_3 CDS 5812041 5812214 5812041 ID=Msa0099600-mRNA-1:cds;Parent=Msa0099600-mRNA-1
chr1_3 CDS 5812737 5813019 5812737 ID=Msa0099600-mRNA-1:cds;Parent=Msa0099600-mRNA-1
Gene Sequence

>Msa0099600

ATGAGGAAGCGACTATTTAATATGATCAATGATCTTCCAACAATCTTTGAAGCTGTTGCAGGGTCTGGTAGCAAACAAGGAAAGGAAAAGTCATCAGTTTCTAATAATAGCAGTACCAAATCCAAATCAAACTCTAAGGTAAGAAAGCTTGAAAAACAAACTAACCACTATCCATTGATTTCTGAACCTCAACCAAAGTTTTCAAAACCAATATCAATGGCAATGCAAATGAAGTTAAAGGACGAGGAGGTTGAGGATGGTGTAGGTGTGGACGAGGAAGAACACGGAGAGACTTTGTGTGGGGCATGTGGAGACAGCTATGCAGCTGATGAGTTCTGGATTTGCAGTGACATTTGTGAGAAATCGTTTCATGGAAAATGCGTGAAGATTACACCGGCAAGGGCTGAGCATATCAAACAGAACAAATGTCCAGCTTGCAGCAACAAGAGAGCTCGCCCTTGA
Protein sequence

>Msa0099600

MRKRLFNMINDLPTIFEAVAGSGSKQGKEKSSVSNNSSTKSKSNSKVRKLEKQTNHYPLISEPQPKFSKPISMAMQMKLKDEEVEDGVGVDEEEHGETLCGACGDSYAADEFWICSDICEKSFHGKCVKITPARAEHIKQNKCPACSNKRARP*