AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0101460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0101460 MtrunA17_Chr1g0154651 90.763 249 23 0 1 249 1 249 5.38e-169 466
Msa0101460 MtrunA17_Chr3g0141401 46.006 313 103 7 1 247 1 313 2.34e-58 187
Msa0101460 MtrunA17_Chr2g0315211 37.931 319 109 13 14 246 6 321 3.39e-25 101
Msa0101460 MtrunA17_Chr5g0403631 43.842 203 68 4 14 175 9 206 8.53e-25 100
Msa0101460 MtrunA17_Chr2g0315201 62.500 88 32 1 14 101 6 92 2.40e-17 79.7
Msa0101460 MtrunA17_Chr5g0408851 54.545 55 25 0 26 80 62 116 1.80e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0101460 AT1G75080.2 45.541 314 93 8 13 248 22 335 4.35e-63 201
Msa0101460 AT1G75080.1 45.541 314 93 8 13 248 22 335 4.35e-63 201
Msa0101460 AT1G19350.3 44.089 313 97 8 13 247 43 355 1.16e-60 195
Msa0101460 AT1G19350.5 44.089 313 97 8 13 247 21 333 1.67e-60 194
Msa0101460 AT1G19350.1 44.089 313 97 8 13 247 21 333 1.67e-60 194
Msa0101460 AT1G19350.6 44.089 313 97 8 13 247 21 333 1.67e-60 194
Msa0101460 AT1G19350.4 44.089 313 97 8 13 247 21 333 1.67e-60 194
Msa0101460 AT3G50750.1 40.559 286 110 9 1 243 1 269 6.59e-34 124
Msa0101460 AT4G36780.1 50.000 164 46 2 14 141 16 179 5.05e-33 121
Msa0101460 AT4G18890.1 36.000 300 102 12 14 246 6 282 1.98e-22 93.6
Msa0101460 AT1G78700.1 32.727 330 107 9 14 243 6 320 1.48e-19 86.3
Msa0101460 AT2G45880.1 51.515 66 32 0 13 78 68 133 7.15e-14 71.2
Msa0101460 AT5G45300.1 54.237 59 27 0 26 84 98 156 8.45e-13 68.2
Msa0101460 AT5G45300.2 54.237 59 27 0 26 84 98 156 8.52e-13 68.2
Msa0101460 AT5G45300.3 54.237 59 27 0 26 84 112 170 8.58e-13 68.2

Find 69 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACAAATGGGACATGGATTT+TGG 0.099494 1_3:-8096126 None:intergenic
ATCGAATATCTTTGATGTTA+TGG 0.271986 1_3:-8095348 None:intergenic
TGATGAGGGATTGGATGATT+TGG 0.284092 1_3:+8096528 Msa0101460:CDS
AGATGCAGCAAAAGGGTAAT+TGG 0.291581 1_3:-8096317 None:intergenic
CTCCATTTCCAATGAATCTA+TGG 0.308568 1_3:+8096288 Msa0101460:CDS
CTTGGGTTCGTTGGGCTAGC+AGG 0.311924 1_3:-8096346 None:intergenic
TTGGAACTCACTCTTGGAAC+TGG 0.334633 1_3:+8096547 Msa0101460:CDS
GAGTAGAGAGGAGGTGTAAC+AGG 0.350527 1_3:-8096226 None:intergenic
GGAAAATGCCATAGATTCAT+TGG 0.351258 1_3:-8096296 None:intergenic
TGCCATAGATTCATTGGAAA+TGG 0.364323 1_3:-8096290 None:intergenic
AACTGGGAAAGGAAAGATTT+AGG 0.375942 1_3:+8096564 Msa0101460:CDS
GGAATTGAAGTTGGAAAAGA+TGG 0.394254 1_3:-8096148 None:intergenic
GATGATTTGGAACTCACTCT+TGG 0.402192 1_3:+8096541 Msa0101460:CDS
TATGAAGGCTTGGGTTCGTT+GGG 0.406355 1_3:-8096354 None:intergenic
GAAGAGGTTGGCACTGCAAA+TGG 0.411998 1_3:-8096409 None:intergenic
TTGGGTTCGTTGGGCTAGCA+GGG 0.427382 1_3:-8096345 None:intergenic
TTGATTGGAGTATGAAGGCT+TGG 0.439266 1_3:-8096364 None:intergenic
CTCTTCATCTTTCAATGCTG+TGG 0.445261 1_3:+8096426 Msa0101460:CDS
AGTTGGAAAAGATGGACAAA+TGG 0.453995 1_3:-8096140 None:intergenic
GCAAAAGGATGGAACTTGAT+TGG 0.457115 1_3:-8096379 None:intergenic
GGGGTAGAAGATGCAGCAAA+AGG 0.462613 1_3:-8096325 None:intergenic
TGGTATGAAGGAATTGAAGT+TGG 0.464795 1_3:-8096157 None:intergenic
GTATGAAGGCTTGGGTTCGT+TGG 0.466162 1_3:-8096355 None:intergenic
GATATCCACAAAGGAGGAAA+AGG 0.466792 1_3:-8096193 None:intergenic
ATGGAGTCCCATGCTGGAAT+TGG 0.471276 1_3:-8096271 None:intergenic
GAAAAGGCTGAGGAGTGAAT+TGG 0.472490 1_3:-8096177 None:intergenic
CTTCTGCACAAAGAGCTTTG+AGG 0.474572 1_3:-8095427 None:intergenic
GGTGTAAGGCTTATTGATGT+TGG 0.478866 1_3:-8096250 None:intergenic
AAAGCTCTTTGTGCAGAAGC+TGG 0.483728 1_3:+8095431 Msa0101460:CDS
TTGGGGTGCAAGTAAAGCCT+TGG 0.488933 1_3:+8096488 Msa0101460:CDS
ATTGATGTTGGTGAGTAGAG+AGG 0.496798 1_3:-8096238 None:intergenic
GCAAATGGAGATTGTGCAAA+AGG 0.501672 1_3:-8096394 None:intergenic
GGAACTTGATTGGAGTATGA+AGG 0.505849 1_3:-8096369 None:intergenic
GTTGGAAAAGATGGACAAAT+GGG 0.506046 1_3:-8096139 None:intergenic
TGGGGTGCAAGTAAAGCCTT+GGG 0.507029 1_3:+8096489 Msa0101460:CDS
CTCACTCTTGGAACTGGGAA+AGG 0.507498 1_3:+8096553 Msa0101460:CDS
TGATTGGAGTATGAAGGCTT+GGG 0.509209 1_3:-8096363 None:intergenic
TTGGAAATGGAGTCCCATGC+TGG 0.515325 1_3:-8096277 None:intergenic
CCCATGCTGGAATTGGTGTA+AGG 0.517184 1_3:-8096264 None:intergenic
ATATGATGAGAGAAAGAAGA+AGG 0.521014 1_3:+8095321 Msa0101460:CDS
GTGCCTGCATCATTGTTTAG+TGG 0.523421 1_3:-8096088 None:intergenic
CTCTTTGTGCAGAAGCTGGT+TGG 0.523500 1_3:+8095435 Msa0101460:CDS
ATGGAGATTGTGCAAAAGGA+TGG 0.524930 1_3:-8096390 None:intergenic
CCTGCATCATTGTTTAGTGG+AGG 0.531453 1_3:-8096085 None:intergenic
TTGTGCAGAAGCTGGTTGGT+CGG 0.532073 1_3:+8095439 Msa0101460:CDS
CTAAGTTACCTTGCGATAGG+TGG 0.533179 1_3:-8095477 None:intergenic
TTCTCTTTCTCCTTCCACGA+TGG 0.536162 1_3:-8095296 None:intergenic
TGGGTTCGTTGGGCTAGCAG+GGG 0.542358 1_3:-8096344 None:intergenic
ATTCTAAGTTACCTTGCGAT+AGG 0.550407 1_3:-8095480 None:intergenic
TCGGAGGAAGCCATCGTGGA+AGG 0.554225 1_3:+8095286 Msa0101460:CDS
TGGAACTCACTCTTGGAACT+GGG 0.557403 1_3:+8096548 Msa0101460:CDS
TGAGGAGTGAATTGGTATGA+AGG 0.559547 1_3:-8096169 None:intergenic
CACAGCATTGAAAGATGAAG+AGG 0.562677 1_3:-8096425 None:intergenic
CACAAAGGAGGAAAAGGCTG+AGG 0.574609 1_3:-8096187 None:intergenic
ATAACATCAAAGATATTCGA+TGG 0.589300 1_3:+8095350 Msa0101460:CDS
GGGTAGAAGATGCAGCAAAA+GGG 0.593286 1_3:-8096324 None:intergenic
CCTCCACTAAACAATGATGC+AGG 0.594195 1_3:+8096085 Msa0101460:CDS
GCCTTACACCAATTCCAGCA+TGG 0.599458 1_3:+8096263 Msa0101460:CDS
CCTTACACCAATTCCAGCAT+GGG 0.605296 1_3:+8096264 Msa0101460:CDS
GGTTGGTCGGTCGAAGAAGA+CGG 0.613610 1_3:+8095452 Msa0101460:CDS
AAAGATGGACAAATGGGACA+TGG 0.629842 1_3:-8096133 None:intergenic
GATGTTGGTGAGTAGAGAGG+AGG 0.631246 1_3:-8096235 None:intergenic
GAATTCGGAGGAAGCCATCG+TGG 0.640888 1_3:+8095282 Msa0101460:CDS
AGACGGAACCACCTATCGCA+AGG 0.647720 1_3:+8095469 Msa0101460:CDS
GCATTGAAAGATGAAGAGGT+TGG 0.651739 1_3:-8096421 None:intergenic
TCGATGGACTTCGAGCTCAA+AGG 0.659335 1_3:+8095366 Msa0101460:CDS
CTGTTTGATATCCACAAAGG+AGG 0.659720 1_3:-8096199 None:intergenic
GCACTGTTTGATATCCACAA+AGG 0.716903 1_3:-8096202 None:intergenic
GAAGCATTGTGACAACAACG+AGG 0.796257 1_3:+8095403 Msa0101460:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTACATATGAAAAGTAT+CGG - chr1_3:8095632-8095651 None:intergenic 15.0%
!!! TAATTACATATGAAAAGTAT+CGG - chr1_3:8095632-8095651 None:intergenic 15.0%
!! AAACTTCAACAAAAAGAATT+CGG + chr1_3:8095267-8095286 Msa0101460:CDS 20.0%
!! TAGTATAAATGGATAGTTAT+AGG - chr1_3:8095550-8095569 None:intergenic 20.0%
!! GAACAATAATGTAGTATAAA+TGG - chr1_3:8095561-8095580 None:intergenic 20.0%
!!! AACATAATTATCTGATCTTT+TGG + chr1_3:8095585-8095604 Msa0101460:intron 20.0%
!!! ATTGTTGTTTTTTGTGAATA+GGG + chr1_3:8096055-8096074 Msa0101460:intron 20.0%
!! AAACTTCAACAAAAAGAATT+CGG + chr1_3:8095267-8095286 Msa0101460:CDS 20.0%
!! TAGTATAAATGGATAGTTAT+AGG - chr1_3:8095550-8095569 None:intergenic 20.0%
!! GAACAATAATGTAGTATAAA+TGG - chr1_3:8095561-8095580 None:intergenic 20.0%
!!! AACATAATTATCTGATCTTT+TGG + chr1_3:8095585-8095604 Msa0101460:intron 20.0%
!!! ATTGTTGTTTTTTGTGAATA+GGG + chr1_3:8096055-8096074 Msa0101460:intron 20.0%
!! TTTTGTTGAAGTTTTTGTGT+CGG - chr1_3:8095261-8095280 None:intergenic 25.0%
! ATCGAATATCTTTGATGTTA+TGG - chr1_3:8095351-8095370 None:intergenic 25.0%
! ATAACATCAAAGATATTCGA+TGG + chr1_3:8095350-8095369 Msa0101460:CDS 25.0%
!! ATCTGATGAATAAGATCTTT+TGG - chr1_3:8095688-8095707 None:intergenic 25.0%
!!! ATACCAATTGATTTTCTTCA+TGG - chr1_3:8095787-8095806 None:intergenic 25.0%
!!! ACATAGTTGTTTTGAACTAA+AGG - chr1_3:8095874-8095893 None:intergenic 25.0%
!!! GATTGTTGTTTTTTGTGAAT+AGG + chr1_3:8096054-8096073 Msa0101460:intron 25.0%
! TGATGAAAAAATTCATGATG+AGG + chr1_3:8096513-8096532 Msa0101460:CDS 25.0%
! GATGAAAAAATTCATGATGA+GGG + chr1_3:8096514-8096533 Msa0101460:CDS 25.0%
!! TTTTGTTGAAGTTTTTGTGT+CGG - chr1_3:8095261-8095280 None:intergenic 25.0%
! ATCGAATATCTTTGATGTTA+TGG - chr1_3:8095351-8095370 None:intergenic 25.0%
! ATAACATCAAAGATATTCGA+TGG + chr1_3:8095350-8095369 Msa0101460:CDS 25.0%
!! ATCTGATGAATAAGATCTTT+TGG - chr1_3:8095688-8095707 None:intergenic 25.0%
!!! ATACCAATTGATTTTCTTCA+TGG - chr1_3:8095787-8095806 None:intergenic 25.0%
!!! ACATAGTTGTTTTGAACTAA+AGG - chr1_3:8095874-8095893 None:intergenic 25.0%
!!! GATTGTTGTTTTTTGTGAAT+AGG + chr1_3:8096054-8096073 Msa0101460:intron 25.0%
! TGATGAAAAAATTCATGATG+AGG + chr1_3:8096513-8096532 Msa0101460:CDS 25.0%
! GATGAAAAAATTCATGATGA+GGG + chr1_3:8096514-8096533 Msa0101460:CDS 25.0%
ATATGATGAGAGAAAGAAGA+AGG + chr1_3:8095321-8095340 Msa0101460:CDS 30.0%
CAAGTAACAAAGAAACAAAG+AGG - chr1_3:8095518-8095537 None:intergenic 30.0%
CTTCCATGAAGAAAATCAAT+TGG + chr1_3:8095781-8095800 Msa0101460:intron 30.0%
AAAGGTTAAAGAAGTTGAAG+TGG - chr1_3:8095856-8095875 None:intergenic 30.0%
! AATAGTGTGCATTTGAAATG+TGG - chr1_3:8095931-8095950 None:intergenic 30.0%
ACTAATGTTGCAAATAAGGT+AGG - chr1_3:8095981-8096000 None:intergenic 30.0%
GTACACTAATGTTGCAAATA+AGG - chr1_3:8095985-8096004 None:intergenic 30.0%
!! ATTTTGGGAATAAGAGAAAG+AGG - chr1_3:8096113-8096132 None:intergenic 30.0%
ATGACAAGTTCAAAAATGCT+TGG + chr1_3:8096469-8096488 Msa0101460:CDS 30.0%
TGACAAGTTCAAAAATGCTT+GGG + chr1_3:8096470-8096489 Msa0101460:CDS 30.0%
!!! TGAATTTTTTCATCAACCCA+AGG - chr1_3:8096508-8096527 None:intergenic 30.0%
AAAAATTCATGATGAGGGAT+TGG + chr1_3:8096519-8096538 Msa0101460:CDS 30.0%
ATATGATGAGAGAAAGAAGA+AGG + chr1_3:8095321-8095340 Msa0101460:CDS 30.0%
CAAGTAACAAAGAAACAAAG+AGG - chr1_3:8095518-8095537 None:intergenic 30.0%
CTTCCATGAAGAAAATCAAT+TGG + chr1_3:8095781-8095800 Msa0101460:intron 30.0%
AAAGGTTAAAGAAGTTGAAG+TGG - chr1_3:8095856-8095875 None:intergenic 30.0%
! AATAGTGTGCATTTGAAATG+TGG - chr1_3:8095931-8095950 None:intergenic 30.0%
ACTAATGTTGCAAATAAGGT+AGG - chr1_3:8095981-8096000 None:intergenic 30.0%
GTACACTAATGTTGCAAATA+AGG - chr1_3:8095985-8096004 None:intergenic 30.0%
!! ATTTTGGGAATAAGAGAAAG+AGG - chr1_3:8096113-8096132 None:intergenic 30.0%
ATGACAAGTTCAAAAATGCT+TGG + chr1_3:8096469-8096488 Msa0101460:CDS 30.0%
TGACAAGTTCAAAAATGCTT+GGG + chr1_3:8096470-8096489 Msa0101460:CDS 30.0%
!!! TGAATTTTTTCATCAACCCA+AGG - chr1_3:8096508-8096527 None:intergenic 30.0%
AAAAATTCATGATGAGGGAT+TGG + chr1_3:8096519-8096538 Msa0101460:CDS 30.0%
CTTCAACAAAAAGAATTCGG+AGG + chr1_3:8095270-8095289 Msa0101460:CDS 35.0%
ATTCTAAGTTACCTTGCGAT+AGG - chr1_3:8095483-8095502 None:intergenic 35.0%
AATTACACAAGCATGCATCA+AGG - chr1_3:8095759-8095778 None:intergenic 35.0%
! ACAAATGGGACATGGATTTT+GGG - chr1_3:8096128-8096147 None:intergenic 35.0%
GTTGGAAAAGATGGACAAAT+GGG - chr1_3:8096142-8096161 None:intergenic 35.0%
AGTTGGAAAAGATGGACAAA+TGG - chr1_3:8096143-8096162 None:intergenic 35.0%
GGAATTGAAGTTGGAAAAGA+TGG - chr1_3:8096151-8096170 None:intergenic 35.0%
TGGTATGAAGGAATTGAAGT+TGG - chr1_3:8096160-8096179 None:intergenic 35.0%
CTCCATTTCCAATGAATCTA+TGG + chr1_3:8096288-8096307 Msa0101460:CDS 35.0%
TGCCATAGATTCATTGGAAA+TGG - chr1_3:8096293-8096312 None:intergenic 35.0%
GGAAAATGCCATAGATTCAT+TGG - chr1_3:8096299-8096318 None:intergenic 35.0%
GACAAGTTCAAAAATGCTTG+GGG + chr1_3:8096471-8096490 Msa0101460:CDS 35.0%
CTTCAACAAAAAGAATTCGG+AGG + chr1_3:8095270-8095289 Msa0101460:CDS 35.0%
ATTCTAAGTTACCTTGCGAT+AGG - chr1_3:8095483-8095502 None:intergenic 35.0%
AATTACACAAGCATGCATCA+AGG - chr1_3:8095759-8095778 None:intergenic 35.0%
! ACAAATGGGACATGGATTTT+GGG - chr1_3:8096128-8096147 None:intergenic 35.0%
GTTGGAAAAGATGGACAAAT+GGG - chr1_3:8096142-8096161 None:intergenic 35.0%
AGTTGGAAAAGATGGACAAA+TGG - chr1_3:8096143-8096162 None:intergenic 35.0%
GGAATTGAAGTTGGAAAAGA+TGG - chr1_3:8096151-8096170 None:intergenic 35.0%
TGGTATGAAGGAATTGAAGT+TGG - chr1_3:8096160-8096179 None:intergenic 35.0%
CTCCATTTCCAATGAATCTA+TGG + chr1_3:8096288-8096307 Msa0101460:CDS 35.0%
TGCCATAGATTCATTGGAAA+TGG - chr1_3:8096293-8096312 None:intergenic 35.0%
GGAAAATGCCATAGATTCAT+TGG - chr1_3:8096299-8096318 None:intergenic 35.0%
GACAAGTTCAAAAATGCTTG+GGG + chr1_3:8096471-8096490 Msa0101460:CDS 35.0%
! GACAAATGGGACATGGATTT+TGG - chr1_3:8096129-8096148 None:intergenic 40.0%
AAAGATGGACAAATGGGACA+TGG - chr1_3:8096136-8096155 None:intergenic 40.0%
TGAGGAGTGAATTGGTATGA+AGG - chr1_3:8096172-8096191 None:intergenic 40.0%
GATATCCACAAAGGAGGAAA+AGG - chr1_3:8096196-8096215 None:intergenic 40.0%
CTGTTTGATATCCACAAAGG+AGG - chr1_3:8096202-8096221 None:intergenic 40.0%
GCACTGTTTGATATCCACAA+AGG - chr1_3:8096205-8096224 None:intergenic 40.0%
! ATTGATGTTGGTGAGTAGAG+AGG - chr1_3:8096241-8096260 None:intergenic 40.0%
GGTGTAAGGCTTATTGATGT+TGG - chr1_3:8096253-8096272 None:intergenic 40.0%
AGATGCAGCAAAAGGGTAAT+TGG - chr1_3:8096320-8096339 None:intergenic 40.0%
TGATTGGAGTATGAAGGCTT+GGG - chr1_3:8096366-8096385 None:intergenic 40.0%
TTGATTGGAGTATGAAGGCT+TGG - chr1_3:8096367-8096386 None:intergenic 40.0%
GGAACTTGATTGGAGTATGA+AGG - chr1_3:8096372-8096391 None:intergenic 40.0%
GCAAAAGGATGGAACTTGAT+TGG - chr1_3:8096382-8096401 None:intergenic 40.0%
ATGGAGATTGTGCAAAAGGA+TGG - chr1_3:8096393-8096412 None:intergenic 40.0%
GCAAATGGAGATTGTGCAAA+AGG - chr1_3:8096397-8096416 None:intergenic 40.0%
GCATTGAAAGATGAAGAGGT+TGG - chr1_3:8096424-8096443 None:intergenic 40.0%
! CACAGCATTGAAAGATGAAG+AGG - chr1_3:8096428-8096447 None:intergenic 40.0%
CTCTTCATCTTTCAATGCTG+TGG + chr1_3:8096426-8096445 Msa0101460:CDS 40.0%
TGATGAGGGATTGGATGATT+TGG + chr1_3:8096528-8096547 Msa0101460:CDS 40.0%
! GATGATTTGGAACTCACTCT+TGG + chr1_3:8096541-8096560 Msa0101460:CDS 40.0%
! GACAAATGGGACATGGATTT+TGG - chr1_3:8096129-8096148 None:intergenic 40.0%
AAAGATGGACAAATGGGACA+TGG - chr1_3:8096136-8096155 None:intergenic 40.0%
TGAGGAGTGAATTGGTATGA+AGG - chr1_3:8096172-8096191 None:intergenic 40.0%
GATATCCACAAAGGAGGAAA+AGG - chr1_3:8096196-8096215 None:intergenic 40.0%
CTGTTTGATATCCACAAAGG+AGG - chr1_3:8096202-8096221 None:intergenic 40.0%
GCACTGTTTGATATCCACAA+AGG - chr1_3:8096205-8096224 None:intergenic 40.0%
! ATTGATGTTGGTGAGTAGAG+AGG - chr1_3:8096241-8096260 None:intergenic 40.0%
GGTGTAAGGCTTATTGATGT+TGG - chr1_3:8096253-8096272 None:intergenic 40.0%
AGATGCAGCAAAAGGGTAAT+TGG - chr1_3:8096320-8096339 None:intergenic 40.0%
TGATTGGAGTATGAAGGCTT+GGG - chr1_3:8096366-8096385 None:intergenic 40.0%
TTGATTGGAGTATGAAGGCT+TGG - chr1_3:8096367-8096386 None:intergenic 40.0%
GGAACTTGATTGGAGTATGA+AGG - chr1_3:8096372-8096391 None:intergenic 40.0%
GCAAAAGGATGGAACTTGAT+TGG - chr1_3:8096382-8096401 None:intergenic 40.0%
ATGGAGATTGTGCAAAAGGA+TGG - chr1_3:8096393-8096412 None:intergenic 40.0%
GCAAATGGAGATTGTGCAAA+AGG - chr1_3:8096397-8096416 None:intergenic 40.0%
GCATTGAAAGATGAAGAGGT+TGG - chr1_3:8096424-8096443 None:intergenic 40.0%
! CACAGCATTGAAAGATGAAG+AGG - chr1_3:8096428-8096447 None:intergenic 40.0%
CTCTTCATCTTTCAATGCTG+TGG + chr1_3:8096426-8096445 Msa0101460:CDS 40.0%
TGATGAGGGATTGGATGATT+TGG + chr1_3:8096528-8096547 Msa0101460:CDS 40.0%
! GATGATTTGGAACTCACTCT+TGG + chr1_3:8096541-8096560 Msa0101460:CDS 40.0%
TTCTCTTTCTCCTTCCACGA+TGG - chr1_3:8095299-8095318 None:intergenic 45.0%
!! GAAGCATTGTGACAACAACG+AGG + chr1_3:8095403-8095422 Msa0101460:CDS 45.0%
CTTCTGCACAAAGAGCTTTG+AGG - chr1_3:8095430-8095449 None:intergenic 45.0%
AAAGCTCTTTGTGCAGAAGC+TGG + chr1_3:8095431-8095450 Msa0101460:CDS 45.0%
CTAAGTTACCTTGCGATAGG+TGG - chr1_3:8095480-8095499 None:intergenic 45.0%
CCTGCATCATTGTTTAGTGG+AGG - chr1_3:8096088-8096107 None:intergenic 45.0%
CCTCCACTAAACAATGATGC+AGG + chr1_3:8096085-8096104 Msa0101460:CDS 45.0%
GTGCCTGCATCATTGTTTAG+TGG - chr1_3:8096091-8096110 None:intergenic 45.0%
GAAAAGGCTGAGGAGTGAAT+TGG - chr1_3:8096180-8096199 None:intergenic 45.0%
CCTTACACCAATTCCAGCAT+GGG + chr1_3:8096264-8096283 Msa0101460:CDS 45.0%
GGGTAGAAGATGCAGCAAAA+GGG - chr1_3:8096327-8096346 None:intergenic 45.0%
TATGAAGGCTTGGGTTCGTT+GGG - chr1_3:8096357-8096376 None:intergenic 45.0%
TTGGAACTCACTCTTGGAAC+TGG + chr1_3:8096547-8096566 Msa0101460:CDS 45.0%
TGGAACTCACTCTTGGAACT+GGG + chr1_3:8096548-8096567 Msa0101460:CDS 45.0%
TTCTCTTTCTCCTTCCACGA+TGG - chr1_3:8095299-8095318 None:intergenic 45.0%
!! GAAGCATTGTGACAACAACG+AGG + chr1_3:8095403-8095422 Msa0101460:CDS 45.0%
CTTCTGCACAAAGAGCTTTG+AGG - chr1_3:8095430-8095449 None:intergenic 45.0%
AAAGCTCTTTGTGCAGAAGC+TGG + chr1_3:8095431-8095450 Msa0101460:CDS 45.0%
CTAAGTTACCTTGCGATAGG+TGG - chr1_3:8095480-8095499 None:intergenic 45.0%
CCTGCATCATTGTTTAGTGG+AGG - chr1_3:8096088-8096107 None:intergenic 45.0%
CCTCCACTAAACAATGATGC+AGG + chr1_3:8096085-8096104 Msa0101460:CDS 45.0%
GTGCCTGCATCATTGTTTAG+TGG - chr1_3:8096091-8096110 None:intergenic 45.0%
GAAAAGGCTGAGGAGTGAAT+TGG - chr1_3:8096180-8096199 None:intergenic 45.0%
CCTTACACCAATTCCAGCAT+GGG + chr1_3:8096264-8096283 Msa0101460:CDS 45.0%
GGGTAGAAGATGCAGCAAAA+GGG - chr1_3:8096327-8096346 None:intergenic 45.0%
TATGAAGGCTTGGGTTCGTT+GGG - chr1_3:8096357-8096376 None:intergenic 45.0%
TTGGAACTCACTCTTGGAAC+TGG + chr1_3:8096547-8096566 Msa0101460:CDS 45.0%
TGGAACTCACTCTTGGAACT+GGG + chr1_3:8096548-8096567 Msa0101460:CDS 45.0%
! TCGATGGACTTCGAGCTCAA+AGG + chr1_3:8095366-8095385 Msa0101460:CDS 50.0%
CTCTTTGTGCAGAAGCTGGT+TGG + chr1_3:8095435-8095454 Msa0101460:CDS 50.0%
TTGTGCAGAAGCTGGTTGGT+CGG + chr1_3:8095439-8095458 Msa0101460:CDS 50.0%
CACAAAGGAGGAAAAGGCTG+AGG - chr1_3:8096190-8096209 None:intergenic 50.0%
! CTCAGCCTTTTCCTCCTTTG+TGG + chr1_3:8096188-8096207 Msa0101460:CDS 50.0%
! GAGTAGAGAGGAGGTGTAAC+AGG - chr1_3:8096229-8096248 None:intergenic 50.0%
! GATGTTGGTGAGTAGAGAGG+AGG - chr1_3:8096238-8096257 None:intergenic 50.0%
GCCTTACACCAATTCCAGCA+TGG + chr1_3:8096263-8096282 Msa0101460:CDS 50.0%
CCCATGCTGGAATTGGTGTA+AGG - chr1_3:8096267-8096286 None:intergenic 50.0%
ATGGAGTCCCATGCTGGAAT+TGG - chr1_3:8096274-8096293 None:intergenic 50.0%
TTGGAAATGGAGTCCCATGC+TGG - chr1_3:8096280-8096299 None:intergenic 50.0%
GGGGTAGAAGATGCAGCAAA+AGG - chr1_3:8096328-8096347 None:intergenic 50.0%
GTATGAAGGCTTGGGTTCGT+TGG - chr1_3:8096358-8096377 None:intergenic 50.0%
GAAGAGGTTGGCACTGCAAA+TGG - chr1_3:8096412-8096431 None:intergenic 50.0%
TTGGGGTGCAAGTAAAGCCT+TGG + chr1_3:8096488-8096507 Msa0101460:CDS 50.0%
TGGGGTGCAAGTAAAGCCTT+GGG + chr1_3:8096489-8096508 Msa0101460:CDS 50.0%
CTCACTCTTGGAACTGGGAA+AGG + chr1_3:8096553-8096572 Msa0101460:CDS 50.0%
! TCGATGGACTTCGAGCTCAA+AGG + chr1_3:8095366-8095385 Msa0101460:CDS 50.0%
CTCTTTGTGCAGAAGCTGGT+TGG + chr1_3:8095435-8095454 Msa0101460:CDS 50.0%
TTGTGCAGAAGCTGGTTGGT+CGG + chr1_3:8095439-8095458 Msa0101460:CDS 50.0%
CACAAAGGAGGAAAAGGCTG+AGG - chr1_3:8096190-8096209 None:intergenic 50.0%
! CTCAGCCTTTTCCTCCTTTG+TGG + chr1_3:8096188-8096207 Msa0101460:CDS 50.0%
! GAGTAGAGAGGAGGTGTAAC+AGG - chr1_3:8096229-8096248 None:intergenic 50.0%
! GATGTTGGTGAGTAGAGAGG+AGG - chr1_3:8096238-8096257 None:intergenic 50.0%
GCCTTACACCAATTCCAGCA+TGG + chr1_3:8096263-8096282 Msa0101460:CDS 50.0%
CCCATGCTGGAATTGGTGTA+AGG - chr1_3:8096267-8096286 None:intergenic 50.0%
ATGGAGTCCCATGCTGGAAT+TGG - chr1_3:8096274-8096293 None:intergenic 50.0%
TTGGAAATGGAGTCCCATGC+TGG - chr1_3:8096280-8096299 None:intergenic 50.0%
GGGGTAGAAGATGCAGCAAA+AGG - chr1_3:8096328-8096347 None:intergenic 50.0%
GTATGAAGGCTTGGGTTCGT+TGG - chr1_3:8096358-8096377 None:intergenic 50.0%
GAAGAGGTTGGCACTGCAAA+TGG - chr1_3:8096412-8096431 None:intergenic 50.0%
TTGGGGTGCAAGTAAAGCCT+TGG + chr1_3:8096488-8096507 Msa0101460:CDS 50.0%
TGGGGTGCAAGTAAAGCCTT+GGG + chr1_3:8096489-8096508 Msa0101460:CDS 50.0%
CTCACTCTTGGAACTGGGAA+AGG + chr1_3:8096553-8096572 Msa0101460:CDS 50.0%
GAATTCGGAGGAAGCCATCG+TGG + chr1_3:8095282-8095301 Msa0101460:CDS 55.0%
! GGTTGGTCGGTCGAAGAAGA+CGG + chr1_3:8095452-8095471 Msa0101460:CDS 55.0%
AGACGGAACCACCTATCGCA+AGG + chr1_3:8095469-8095488 Msa0101460:CDS 55.0%
!! TTGGGTTCGTTGGGCTAGCA+GGG - chr1_3:8096348-8096367 None:intergenic 55.0%
GAATTCGGAGGAAGCCATCG+TGG + chr1_3:8095282-8095301 Msa0101460:CDS 55.0%
! GGTTGGTCGGTCGAAGAAGA+CGG + chr1_3:8095452-8095471 Msa0101460:CDS 55.0%
AGACGGAACCACCTATCGCA+AGG + chr1_3:8095469-8095488 Msa0101460:CDS 55.0%
!! TTGGGTTCGTTGGGCTAGCA+GGG - chr1_3:8096348-8096367 None:intergenic 55.0%
TCGGAGGAAGCCATCGTGGA+AGG + chr1_3:8095286-8095305 Msa0101460:CDS 60.0%
!! TGGGTTCGTTGGGCTAGCAG+GGG - chr1_3:8096347-8096366 None:intergenic 60.0%
!! CTTGGGTTCGTTGGGCTAGC+AGG - chr1_3:8096349-8096368 None:intergenic 60.0%
TCGGAGGAAGCCATCGTGGA+AGG + chr1_3:8095286-8095305 Msa0101460:CDS 60.0%
!! TGGGTTCGTTGGGCTAGCAG+GGG - chr1_3:8096347-8096366 None:intergenic 60.0%
!! CTTGGGTTCGTTGGGCTAGC+AGG - chr1_3:8096349-8096368 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 8095251 8096585 8095251 ID=Msa0101460;Name=Msa0101460
chr1_3 mRNA 8095251 8096585 8095251 ID=Msa0101460-mRNA-1;Parent=Msa0101460;Name=Msa0101460-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|0|0|1|1|1|2|0|249
chr1_3 exon 8095251 8095490 8095251 ID=Msa0101460-mRNA-1:exon:3204;Parent=Msa0101460-mRNA-1
chr1_3 exon 8096076 8096585 8096076 ID=Msa0101460-mRNA-1:exon:3205;Parent=Msa0101460-mRNA-1
chr1_3 CDS 8095251 8095490 8095251 ID=Msa0101460-mRNA-1:cds;Parent=Msa0101460-mRNA-1
chr1_3 CDS 8096076 8096585 8096076 ID=Msa0101460-mRNA-1:cds;Parent=Msa0101460-mRNA-1
Gene Sequence

>Msa0101460

ATGGCTTCCGACACAAAAACTTCAACAAAAAGAATTCGGAGGAAGCCATCGTGGAAGGAGAAAGAGAAAAATATGATGAGAGAAAGAAGAAGGAGAGCCATAACATCAAAGATATTCGATGGACTTCGAGCTCAAAGGAGCTACAATTTATCGAAGCATTGTGACAACAACGAGGTCCTCAAAGCTCTTTGTGCAGAAGCTGGTTGGTCGGTCGAAGAAGACGGAACCACCTATCGCAAGGGTTACAATCCTCCACTAAACAATGATGCAGGCACCTCTTTCTCTTATTCCCAAAATCCATGTCCCATTTGTCCATCTTTTCCAACTTCAATTCCTTCATACCAATTCACTCCTCAGCCTTTTCCTCCTTTGTGGATATCAAACAGTGCTCCTGTTACACCTCCTCTCTACTCACCAACATCAATAAGCCTTACACCAATTCCAGCATGGGACTCCATTTCCAATGAATCTATGGCATTTTCCAATTACCCTTTTGCTGCATCTTCTACCCCTGCTAGCCCAACGAACCCAAGCCTTCATACTCCAATCAAGTTCCATCCTTTTGCACAATCTCCATTTGCAGTGCCAACCTCTTCATCTTTCAATGCTGTGGCTGATAACTCAATCAGTGAGATGACAAGTTCAAAAATGCTTGGGGTGCAAGTAAAGCCTTGGGTTGATGAAAAAATTCATGATGAGGGATTGGATGATTTGGAACTCACTCTTGGAACTGGGAAAGGAAAGATTTAG
Protein sequence

>Msa0101460

MASDTKTSTKRIRRKPSWKEKEKNMMRERRRRAITSKIFDGLRAQRSYNLSKHCDNNEVLKALCAEAGWSVEEDGTTYRKGYNPPLNNDAGTSFSYSQNPCPICPSFPTSIPSYQFTPQPFPPLWISNSAPVTPPLYSPTSISLTPIPAWDSISNESMAFSNYPFAASSTPASPTNPSLHTPIKFHPFAQSPFAVPTSSSFNAVADNSISEMTSSKMLGVQVKPWVDEKIHDEGLDDLELTLGTGKGKI*