AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0106680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0106680 MtrunA17_Chr1g0162721 95.486 288 11 1 56 343 1 286 0.0 570
Msa0106680 MtrunA17_Chr2g0302481 58.163 196 47 5 150 343 5 167 3.32e-60 191
Msa0106680 MtrunA17_Chr2g0302501 51.829 164 31 7 46 207 10 127 9.14e-40 137
Msa0106680 MtrunA17_Chr4g0065261 50.000 90 24 2 83 152 1 89 3.20e-19 84.0
Msa0106680 MtrunA17_Chr4g0065261 57.353 68 28 1 276 343 88 154 3.52e-15 72.8
Msa0106680 MtrunA17_Chr8g0373571 47.887 71 35 1 275 343 254 324 3.32e-15 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0106680 AT4G36730.2 24.535 269 181 4 85 343 28 284 1.98e-20 90.9
Msa0106680 AT4G36730.1 24.354 271 181 5 85 343 28 286 1.13e-18 85.9
Msa0106680 AT2G35530.1 40.000 65 39 0 279 343 305 369 4.96e-11 63.9

Find 67 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTGGTCTGGCTCAGAATT+TGG 0.096203 1_3:-16258848 Msa0106680:CDS
AACAACTATGTGGGGTTTAT+TGG 0.126290 1_3:+16259833 None:intergenic
GAAATGGTACACAGAAATTT+TGG 0.193208 1_3:-16259534 Msa0106680:CDS
GATTTATGCTTCTTAGTTAA+AGG 0.201185 1_3:+16259055 None:intergenic
TCTGGTCTGGCTCAGAATTT+GGG 0.206123 1_3:-16258847 Msa0106680:CDS
ACTGGGGAATCAAGAGAATT+TGG 0.246551 1_3:-16259453 Msa0106680:CDS
TCCCCAGTCATTAAACTTAC+AGG 0.268465 1_3:+16259468 None:intergenic
TAGCAAATAAGTTGAAATCT+TGG 0.305874 1_3:+16259502 None:intergenic
CTCCTTCAGTTTCATTTCTC+TGG 0.315527 1_3:-16261351 Msa0106680:intron
AAACAGTGTTTGGGCTTTAT+CGG 0.324427 1_3:-16261463 Msa0106680:CDS
ACATAAGAAAAGAAAGAAAA+AGG 0.328524 1_3:-16257687 Msa0106680:CDS
TGAGGTAACATAGGTTTGTT+TGG 0.346442 1_3:+16260244 None:intergenic
TTGATGAATAAAACAGTGTT+TGG 0.359121 1_3:-16261473 Msa0106680:CDS
CACAAGTTTCTCGATCTTGA+TGG 0.368390 1_3:-16260437 Msa0106680:CDS
AAACAGGCTTATTACAATCC+TGG 0.410680 1_3:-16260093 Msa0106680:intron
TTCCTTGTAGAAAATTCATC+TGG 0.413848 1_3:-16258865 Msa0106680:intron
TCAGTACATCTTGCAAAATC+AGG 0.425666 1_3:-16260013 Msa0106680:intron
AAGATCAAAGATAAAGAAAC+AGG 0.437513 1_3:-16257641 Msa0106680:intron
AAGGAAGAATCCACTCCGTT+TGG 0.441874 1_3:-16260412 Msa0106680:CDS
TGTAGAAAATTCATCTGGTC+TGG 0.448465 1_3:-16258860 Msa0106680:intron
TGATGAATAAAACAGTGTTT+GGG 0.449654 1_3:-16261472 Msa0106680:CDS
ACAAGTTTCTCGATCTTGAT+GGG 0.458271 1_3:-16260436 Msa0106680:CDS
ACAAACCTATGTTACCTCAT+TGG 0.461450 1_3:-16260240 Msa0106680:CDS
TAAGCTTTCTGAGGAATGTC+TGG 0.463695 1_3:-16256428 Msa0106680:CDS
TGGAAGACCTTACAATTTCA+CGG 0.479597 1_3:-16260392 Msa0106680:CDS
TAATAAGATAGACACTCTTA+AGG 0.488014 1_3:-16256482 Msa0106680:CDS
GCTTAGCAAGCGTTTGTGCT+AGG 0.489007 1_3:+16256446 None:intergenic
GTTAAAATGATGGACCTCAA+TGG 0.494777 1_3:+16256384 None:intergenic
AACAGGCTTATTACAATCCT+GGG 0.495397 1_3:-16260092 Msa0106680:intron
TGAGGCACCGTGAAATTGTA+AGG 0.513205 1_3:+16260385 None:intergenic
GGTTTATTGGACAGCCTCAC+GGG 0.515395 1_3:+16259846 None:intergenic
CAACCTGTAAGTTTAATGAC+TGG 0.518291 1_3:-16259471 Msa0106680:CDS
AACCTGTAAGTTTAATGACT+GGG 0.521729 1_3:-16259470 Msa0106680:CDS
GGGTTTATTGGACAGCCTCA+CGG 0.528698 1_3:+16259845 None:intergenic
CTTCAACATGATGCTCTACA+TGG 0.529457 1_3:-16259870 Msa0106680:CDS
ATATTGATTCAGGAAGCTAC+AGG 0.541673 1_3:-16260463 Msa0106680:intron
ATGTACTGATGATACAAGTA+AGG 0.542138 1_3:+16260027 None:intergenic
TCATTGGACTACAGCTATGC+AGG 0.554356 1_3:-16260224 Msa0106680:intron
GAACAAATTGTACCTCATTG+AGG 0.556643 1_3:+16259392 None:intergenic
GGCTCACCTCAACAACTATG+TGG 0.564253 1_3:+16259823 None:intergenic
AAGGAGCAGCTGCAAGTGGA+TGG 0.564358 1_3:+16260046 None:intergenic
GAATTTGGGGACAGTCATCA+AGG 0.574576 1_3:-16258833 Msa0106680:CDS
TCTAACATGATTACTGCGGA+TGG 0.576063 1_3:-16259034 Msa0106680:intron
GCTCACCTCAACAACTATGT+GGG 0.580762 1_3:+16259824 None:intergenic
GTAGTCCAATGAGGTAACAT+AGG 0.581289 1_3:+16260235 None:intergenic
AAAGAGGGGAGTTAAAATGA+TGG 0.581509 1_3:+16256374 None:intergenic
AAATTGTAAGGTCTTCCAAA+CGG 0.581928 1_3:+16260397 None:intergenic
AAGTAAGGAGCAGCTGCAAG+TGG 0.592524 1_3:+16260042 None:intergenic
TTTCTCGATCTTGATGGGGA+AGG 0.594200 1_3:-16260431 Msa0106680:CDS
TAAACCCCACATAGTTGTTG+AGG 0.602835 1_3:-16259829 Msa0106680:intron
AACGCTTGCTAAGCTTTCTG+AGG 0.605311 1_3:-16256437 Msa0106680:CDS
CAAGTTTCTCGATCTTGATG+GGG 0.608143 1_3:-16260435 Msa0106680:CDS
AAAGTATTTGGAGTAGACCC+AGG 0.610691 1_3:+16260075 None:intergenic
CAATGAAAATGATTCCATTG+AGG 0.612331 1_3:-16256398 Msa0106680:CDS
TACCAGAGAAATGAAACTGA+AGG 0.623020 1_3:+16261349 None:intergenic
CTGGTCTGGCTCAGAATTTG+GGG 0.624581 1_3:-16258846 Msa0106680:CDS
AACGGTGAGAGAATGTTCTG+TGG 0.639951 1_3:+16261387 None:intergenic
ACCTGTAAGTTTAATGACTG+GGG 0.644193 1_3:-16259469 Msa0106680:CDS
CTCACCTCAACAACTATGTG+GGG 0.660296 1_3:+16259825 None:intergenic
GATTTACTGACATCATCTTG+AGG 0.660566 1_3:+16260367 None:intergenic
TGTGTGCACAGTGCAGCAAG+TGG 0.664216 1_3:-16259314 Msa0106680:intron
GTAAGGTCTTCCAAACGGAG+TGG 0.675114 1_3:+16260402 None:intergenic
TAAATCTAACATGATTACTG+CGG 0.677606 1_3:-16259038 Msa0106680:CDS
TGCATAGCTGTAGTCCAATG+AGG 0.677790 1_3:+16260226 None:intergenic
AACATCGACATTAACAACGT+CGG 0.677992 1_3:+16258801 None:intergenic
ATGCTCTACATGGTCCCGTG+AGG 0.706895 1_3:-16259860 Msa0106680:CDS
ACAGGGATGGAACCTCAATG+AGG 0.714168 1_3:-16259404 Msa0106680:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAATAATATTAAAGCAAAT+AGG + chr1_3:16256560-16256579 None:intergenic 15.0%
!!! AATATTATTGATTGATTGAT+TGG - chr1_3:16256569-16256588 Msa0106680:intron 15.0%
!! TTTAAAAATTCAGTTTCAAT+TGG - chr1_3:16256744-16256763 Msa0106680:intron 15.0%
!!! GTATGATTATGATATTTTTT+AGG - chr1_3:16256768-16256787 Msa0106680:intron 15.0%
!! TAATTGAAATATTGAATACA+CGG - chr1_3:16256815-16256834 Msa0106680:intron 15.0%
!!! TTCATAATTGTACATTATTT+TGG - chr1_3:16257352-16257371 Msa0106680:intron 15.0%
!!! TCTTATTTTAAATTTCAAAC+AGG - chr1_3:16257748-16257767 Msa0106680:intron 15.0%
!!! TGAAATTTTCATATTTCTTT+AGG - chr1_3:16258151-16258170 Msa0106680:intron 15.0%
!!! GAAATTTTCATATTTCTTTA+GGG - chr1_3:16258152-16258171 Msa0106680:intron 15.0%
!!! ATAATTTTTTATTCAGACTT+GGG + chr1_3:16258524-16258543 None:intergenic 15.0%
!!! TTTAAAATTATTCGATAGTA+TGG - chr1_3:16258728-16258747 Msa0106680:intron 15.0%
!! ATTTAATAAAATAGAAGTGA+TGG - chr1_3:16259536-16259555 Msa0106680:CDS 15.0%
!!! CAATATTTTTTGTAATTATG+AGG + chr1_3:16260304-16260323 None:intergenic 15.0%
!!! AGTTGAAAAAAAAACTTTTT+GGG + chr1_3:16260425-16260444 None:intergenic 15.0%
!! TATTAAATAAAATTAGAGTG+AGG + chr1_3:16260448-16260467 None:intergenic 15.0%
!! AAATGTCAATTAGAAAAAAT+AGG + chr1_3:16260549-16260568 None:intergenic 15.0%
!!! TTTTATAAATTATGTAATCC+AGG + chr1_3:16261265-16261284 None:intergenic 15.0%
!!! ACAAATAATTGTTTTCGATA+GGG + chr1_3:16256609-16256628 None:intergenic 20.0%
!!! GGATTTTTTTACAAAATTGA+AGG - chr1_3:16256864-16256883 Msa0106680:intron 20.0%
!! AATAAAGATTTGTACAAGAA+TGG + chr1_3:16257062-16257081 None:intergenic 20.0%
!!! TTGAAGGAAAATTTTCAATT+TGG - chr1_3:16257191-16257210 Msa0106680:intron 20.0%
!!! GAAAATTTTCAATTTGGTAT+TGG - chr1_3:16257197-16257216 Msa0106680:intron 20.0%
!!! TTAAAAGCAAATATTGATTC+AGG - chr1_3:16257384-16257403 Msa0106680:intron 20.0%
!! ATATATCTATTGAAAGGTTT+AGG + chr1_3:16258058-16258077 None:intergenic 20.0%
!! CAATGAATATATCTATTGAA+AGG + chr1_3:16258064-16258083 None:intergenic 20.0%
!!! ATATGAATGTTTATGCATTT+TGG - chr1_3:16258275-16258294 Msa0106680:intron 20.0%
!!! CATAATTTTTTATTCAGACT+TGG + chr1_3:16258525-16258544 None:intergenic 20.0%
!! AAGAAATGAGTAGTTTATTA+AGG - chr1_3:16258704-16258723 Msa0106680:intron 20.0%
!!! TATGTTTTATTATGTTCAGA+AGG - chr1_3:16258912-16258931 Msa0106680:intron 20.0%
!! GTAACATAGATTTAGAAAAA+TGG - chr1_3:16259647-16259666 Msa0106680:intron 20.0%
!!! GATCATACAGATATTTTTAT+AGG + chr1_3:16259851-16259870 None:intergenic 20.0%
!!! CATTCTAGCTAAATATATAT+GGG - chr1_3:16260031-16260050 Msa0106680:CDS 20.0%
!!! ATTCTAGCTAAATATATATG+GGG - chr1_3:16260032-16260051 Msa0106680:CDS 20.0%
!! AAGTTTAATTCTTAACTATC+TGG - chr1_3:16260093-16260112 Msa0106680:intron 20.0%
!! ACATAAGAAAAGAAAGAAAA+AGG - chr1_3:16260170-16260189 Msa0106680:intron 20.0%
!! AATGTCTATGAATTTGATAA+TGG - chr1_3:16260325-16260344 Msa0106680:intron 20.0%
!!! GAGTTGAAAAAAAAACTTTT+TGG + chr1_3:16260426-16260445 None:intergenic 20.0%
!! CAAATAAGTACAAGTATATT+GGG + chr1_3:16260675-16260694 None:intergenic 20.0%
!! TGTTATGTGCTTTAATAAAT+AGG + chr1_3:16260712-16260731 None:intergenic 20.0%
!! TACATGATTATGATTATACA+TGG - chr1_3:16261026-16261045 Msa0106680:intron 20.0%
!!! TAGTAATGGAAGATAATATT+TGG + chr1_3:16261166-16261185 None:intergenic 20.0%
!! GACAAATAATTGTTTTCGAT+AGG + chr1_3:16256610-16256629 None:intergenic 25.0%
! CCAATTAAAAAACACAATGT+AGG + chr1_3:16256846-16256865 None:intergenic 25.0%
!!! CCTACATTGTGTTTTTTAAT+TGG - chr1_3:16256843-16256862 Msa0106680:intron 25.0%
! ATTGAAGGATTTGTAATTGT+TGG - chr1_3:16256879-16256898 Msa0106680:intron 25.0%
!! TGTGTATACATTTTCTTGTT+TGG - chr1_3:16257252-16257271 Msa0106680:intron 25.0%
!!! TATACATTTTCTTGTTTGGT+TGG - chr1_3:16257256-16257275 Msa0106680:intron 25.0%
! ACATAACGTCAAAGAATATT+TGG + chr1_3:16257333-16257352 None:intergenic 25.0%
! GATGTCAGTAAATCATATTA+TGG - chr1_3:16257499-16257518 Msa0106680:intron 25.0%
!!! GGATGGTTATAAAAAGTATT+TGG + chr1_3:16257797-16257816 None:intergenic 25.0%
! ATACATATTACTGCTTTGTA+CGG - chr1_3:16257874-16257893 Msa0106680:intron 25.0%
!! TGTCTATGAAATTCATTTTC+TGG - chr1_3:16258113-16258132 Msa0106680:intron 25.0%
!!! ATTTTCATATTTCTTTAGGG+TGG - chr1_3:16258155-16258174 Msa0106680:intron 25.0%
!!! TTTTCATATTTCTTTAGGGT+GGG - chr1_3:16258156-16258175 Msa0106680:intron 25.0%
! TAAATCATTCCACTAGTAAT+TGG + chr1_3:16258192-16258211 None:intergenic 25.0%
! AACTCATACACAATTCATTA+TGG + chr1_3:16258244-16258263 None:intergenic 25.0%
!!! AGTTGAAATCTTGGTTTTTT+TGG + chr1_3:16258349-16258368 None:intergenic 25.0%
!! TAGCAAATAAGTTGAAATCT+TGG + chr1_3:16258358-16258377 None:intergenic 25.0%
! GATTTATGCTTCTTAGTTAA+AGG + chr1_3:16258805-16258824 None:intergenic 25.0%
! TAAATCTAACATGATTACTG+CGG - chr1_3:16258819-16258838 Msa0106680:CDS 25.0%
! TGATGCTCAACTTAAAAATA+TGG - chr1_3:16259078-16259097 Msa0106680:intron 25.0%
! ACATAGATTTAGAAAAATGG+AGG - chr1_3:16259650-16259669 Msa0106680:intron 25.0%
! TATACATACAATATGTGATG+GGG + chr1_3:16259748-16259767 None:intergenic 25.0%
! CTATACATACAATATGTGAT+GGG + chr1_3:16259749-16259768 None:intergenic 25.0%
! TCTATACATACAATATGTGA+TGG + chr1_3:16259750-16259769 None:intergenic 25.0%
!!! ACAGATATTTTTATAGGACT+AGG + chr1_3:16259845-16259864 None:intergenic 25.0%
! TTCTATAACTATTGCCAATT+TGG + chr1_3:16259886-16259905 None:intergenic 25.0%
! ATATATTTAGCTAGAATGCA+GGG + chr1_3:16260029-16260048 None:intergenic 25.0%
! TATATATTTAGCTAGAATGC+AGG + chr1_3:16260030-16260049 None:intergenic 25.0%
!! GCATTCTAGCTAAATATATA+TGG - chr1_3:16260030-16260049 Msa0106680:CDS 25.0%
! AGAAAGTAAATTCCTTCTTA+AGG - chr1_3:16260065-16260084 Msa0106680:CDS 25.0%
! AAGATCAAAGATAAAGAAAC+AGG - chr1_3:16260216-16260235 Msa0106680:intron 25.0%
! ATGAGGATAACTGTATATAA+CGG + chr1_3:16260287-16260306 None:intergenic 25.0%
! CCAAATAAGTACAAGTATAT+TGG + chr1_3:16260676-16260695 None:intergenic 25.0%
! CCAATATACTTGTACTTATT+TGG - chr1_3:16260673-16260692 Msa0106680:intron 25.0%
! TATGTGCTTTAATAAATAGG+TGG + chr1_3:16260709-16260728 None:intergenic 25.0%
! AAGTTAAGAATATCGTTTCT+TGG + chr1_3:16260814-16260833 None:intergenic 25.0%
!! AACGATATTCTTAACTTGAT+TGG - chr1_3:16260817-16260836 Msa0106680:intron 25.0%
! ACAATCATGTTCCAAATAAA+GGG + chr1_3:16261003-16261022 None:intergenic 25.0%
! TACAATCATGTTCCAAATAA+AGG + chr1_3:16261004-16261023 None:intergenic 25.0%
!! TTTATTTGGAACATGATTGT+AGG - chr1_3:16261003-16261022 Msa0106680:intron 25.0%
! TGATTATGATTATACATGGT+AGG - chr1_3:16261030-16261049 Msa0106680:intron 25.0%
! GATTATGATTATACATGGTA+GGG - chr1_3:16261031-16261050 Msa0106680:intron 25.0%
! AAGATAATATTTGGCTGTTT+GGG + chr1_3:16261157-16261176 None:intergenic 25.0%
! ATTATCTTCCATTACTATTG+TGG - chr1_3:16261169-16261188 Msa0106680:intron 25.0%
! TATAAATTATGTAATCCAGG+AGG + chr1_3:16261262-16261281 None:intergenic 25.0%
! TAATAAGATAGACACTCTTA+AGG - chr1_3:16261375-16261394 Msa0106680:CDS 25.0%
!! TTAGGTTTTCTTGTTGATAG+TGG - chr1_3:16256786-16256805 Msa0106680:intron 30.0%
CAAGAAAAGGAAAGAAAAAG+TGG + chr1_3:16257005-16257024 None:intergenic 30.0%
CTTGCTTGATTAACAAGAAA+AGG + chr1_3:16257018-16257037 None:intergenic 30.0%
CTTGTTAATCAAGCAAGAAA+AGG - chr1_3:16257021-16257040 Msa0106680:intron 30.0%
AAAGATTTGTACAAGAATGG+AGG + chr1_3:16257059-16257078 None:intergenic 30.0%
AGTATTGTCAATGACAATTG+CGG + chr1_3:16257158-16257177 None:intergenic 30.0%
AAATTGTAAGGTCTTCCAAA+CGG + chr1_3:16257463-16257482 None:intergenic 30.0%
! ATGTACTGATGATACAAGTA+AGG + chr1_3:16257833-16257852 None:intergenic 30.0%
!! CTTTCACATTCTTTGTTGTT+TGG - chr1_3:16257944-16257963 Msa0106680:intron 30.0%
!! TTTCATATTTCTTTAGGGTG+GGG - chr1_3:16258157-16258176 Msa0106680:intron 30.0%
!! AATGTTTATGCATTTTGGTC+AGG - chr1_3:16258280-16258299 Msa0106680:intron 30.0%
! GAAATGGTACACAGAAATTT+TGG - chr1_3:16258323-16258342 Msa0106680:intron 30.0%
AACCTGTAAGTTTAATGACT+GGG - chr1_3:16258387-16258406 Msa0106680:intron 30.0%
TTCCTTGTAGAAAATTCATC+TGG - chr1_3:16258992-16259011 Msa0106680:intron 30.0%
! ATGGTATTGTAACTTTTGTG+TGG - chr1_3:16259097-16259116 Msa0106680:intron 30.0%
ATCTCATTCACACATGTTAT+AGG - chr1_3:16259209-16259228 Msa0106680:intron 30.0%
! TAGGTAGTGTTGTTAAATAG+AGG - chr1_3:16259228-16259247 Msa0106680:intron 30.0%
AATGGCAGTTCATTCAAATT+CGG + chr1_3:16259275-16259294 None:intergenic 30.0%
ACTAACAAATGTCTAACACT+CGG - chr1_3:16259598-16259617 Msa0106680:intron 30.0%
ATACATACAATATGTGATGG+GGG + chr1_3:16259747-16259766 None:intergenic 30.0%
ATATCTAACAAGATTCGAGA+TGG + chr1_3:16259792-16259811 None:intergenic 30.0%
ATCTTGTTAGATATGCTTGT+TGG - chr1_3:16259798-16259817 Msa0106680:intron 30.0%
! TTTTTATAGGACTAGGTAGA+TGG + chr1_3:16259838-16259857 None:intergenic 30.0%
TAAGTTCTTACAAGATCAGT+TGG + chr1_3:16259924-16259943 None:intergenic 30.0%
TAAACTTAGCAACCTTAAGA+AGG + chr1_3:16260080-16260099 None:intergenic 30.0%
AAAGTCTAGCATTTCCTATT+TGG - chr1_3:16260119-16260138 Msa0106680:intron 30.0%
AAAAAGAAATGCAGCCAAAT+AGG + chr1_3:16260136-16260155 None:intergenic 30.0%
AAAGAAAAAGGTTGTCAAAC+AGG - chr1_3:16260182-16260201 Msa0106680:intron 30.0%
ATGTAAAAAGAAAAAGACGC+TGG - chr1_3:16260368-16260387 Msa0106680:intron 30.0%
!! ACGCTGGTTATTTTCATTAT+TGG - chr1_3:16260384-16260403 Msa0106680:CDS 30.0%
TAGCATAAAACAGCTGTATT+TGG - chr1_3:16260516-16260535 Msa0106680:intron 30.0%
AAGGTCATGATAAAAAGCAA+AGG + chr1_3:16260595-16260614 None:intergenic 30.0%
! AAATGCATGTTTAGGACATA+AGG + chr1_3:16260614-16260633 None:intergenic 30.0%
GTCCTAAACATGCATTTAAA+TGG - chr1_3:16260617-16260636 Msa0106680:intron 30.0%
! TGCCATTTAAATGCATGTTT+AGG + chr1_3:16260622-16260641 None:intergenic 30.0%
TAAGTACAAGTATATTGGGT+AGG + chr1_3:16260671-16260690 None:intergenic 30.0%
! TGCATTTTAGACAGTAAAGA+TGG - chr1_3:16260750-16260769 Msa0106680:intron 30.0%
TTGAAACTGATCATCAATGA+AGG + chr1_3:16260896-16260915 None:intergenic 30.0%
CAACAACATTTCCCTTTATT+TGG - chr1_3:16260989-16261008 Msa0106680:intron 30.0%
! ATTAGGTGGATTCAATTACA+TGG + chr1_3:16261076-16261095 None:intergenic 30.0%
ATGTAATTGAATCCACCTAA+TGG - chr1_3:16261075-16261094 Msa0106680:intron 30.0%
! TGTTGTTGTTGTATGATCAT+AGG - chr1_3:16261121-16261140 Msa0106680:intron 30.0%
GAAGATAATATTTGGCTGTT+TGG + chr1_3:16261158-16261177 None:intergenic 30.0%
TTCAACTTCCACAATAGTAA+TGG + chr1_3:16261180-16261199 None:intergenic 30.0%
! CTCAATGGAATCATTTTCAT+TGG + chr1_3:16261461-16261480 None:intergenic 30.0%
CAATGAAAATGATTCCATTG+AGG - chr1_3:16261459-16261478 Msa0106680:intron 30.0%
! AAACAGTGTTTGGGCTTTAT+CGG - chr1_3:16256394-16256413 Msa0106680:CDS 35.0%
AGGAGTTCGAAAAAACGAAA+CGG + chr1_3:16256491-16256510 None:intergenic 35.0%
TACCAGAGAAATGAAACTGA+AGG + chr1_3:16256511-16256530 None:intergenic 35.0%
! CTTTCATCTGAGATGAACTT+CGG - chr1_3:16256638-16256657 Msa0106680:intron 35.0%
TCAATGACAATTGCGGAAAA+TGG + chr1_3:16257151-16257170 None:intergenic 35.0%
GCAATTGTCATTGACAATAC+TGG - chr1_3:16257157-16257176 Msa0106680:intron 35.0%
ACTGGTCAGGTTTAAATTGA+AGG - chr1_3:16257175-16257194 Msa0106680:intron 35.0%
!! ATATTGATTCAGGAAGCTAC+AGG - chr1_3:16257394-16257413 Msa0106680:intron 35.0%
ACAAGTTTCTCGATCTTGAT+GGG - chr1_3:16257421-16257440 Msa0106680:intron 35.0%
TGGAAGACCTTACAATTTCA+CGG - chr1_3:16257465-16257484 Msa0106680:intron 35.0%
GATTTACTGACATCATCTTG+AGG + chr1_3:16257493-16257512 None:intergenic 35.0%
! TGAGGTAACATAGGTTTGTT+TGG + chr1_3:16257616-16257635 None:intergenic 35.0%
ACAAACCTATGTTACCTCAT+TGG - chr1_3:16257617-16257636 Msa0106680:intron 35.0%
TACTCTGAACCAATTTGTGA+TGG - chr1_3:16257670-16257689 Msa0106680:CDS 35.0%
ACCAATTTGTGATGGTATCT+TGG - chr1_3:16257678-16257697 Msa0106680:CDS 35.0%
ACCAAGATACCATCACAAAT+TGG + chr1_3:16257682-16257701 None:intergenic 35.0%
AAACAGGCTTATTACAATCC+TGG - chr1_3:16257764-16257783 Msa0106680:intron 35.0%
AACAGGCTTATTACAATCCT+GGG - chr1_3:16257765-16257784 Msa0106680:intron 35.0%
TCAGTACATCTTGCAAAATC+AGG - chr1_3:16257844-16257863 Msa0106680:intron 35.0%
AACAACTATGTGGGGTTTAT+TGG + chr1_3:16258027-16258046 None:intergenic 35.0%
!!! TGCAAAGGCTTTTTCAGAAA+TGG - chr1_3:16258307-16258326 Msa0106680:intron 35.0%
CAACCTGTAAGTTTAATGAC+TGG - chr1_3:16258386-16258405 Msa0106680:intron 35.0%
ACCTGTAAGTTTAATGACTG+GGG - chr1_3:16258388-16258407 Msa0106680:intron 35.0%
GAACAAATTGTACCTCATTG+AGG + chr1_3:16258468-16258487 None:intergenic 35.0%
GTCTAATTACATCCTTTCTG+TGG - chr1_3:16258770-16258789 Msa0106680:intron 35.0%
! GACCAGATGAATTTTCTACA+AGG + chr1_3:16258997-16259016 None:intergenic 35.0%
TGTAGAAAATTCATCTGGTC+TGG - chr1_3:16258997-16259016 Msa0106680:intron 35.0%
AACATCGACATTAACAACGT+CGG + chr1_3:16259059-16259078 None:intergenic 35.0%
CATTATGATATGCACACACT+TGG - chr1_3:16259183-16259202 Msa0106680:intron 35.0%
CAATCGCAGATTACACAAAA+TGG + chr1_3:16259293-16259312 None:intergenic 35.0%
CGATTGAAAACACTAGTTAC+AGG - chr1_3:16259307-16259326 Msa0106680:intron 35.0%
!!! ACGATGTAGTGTGGTTTTAT+TGG + chr1_3:16259454-16259473 None:intergenic 35.0%
! AAGTGATGGAATGTAACCAT+AGG - chr1_3:16259550-16259569 Msa0106680:CDS 35.0%
TTGTACGAAACTAACACCTA+TGG + chr1_3:16259569-16259588 None:intergenic 35.0%
! TGTTAGTTTCGTACAAGTGT+CGG - chr1_3:16259573-16259592 Msa0106680:intron 35.0%
ACATCGACACTTCAAATTGA+AGG + chr1_3:16259629-16259648 None:intergenic 35.0%
CTTAAGCTTTCAAGAGAATC+TGG + chr1_3:16259695-16259714 None:intergenic 35.0%
TAACTATTGCCAATTTGGCA+TGG + chr1_3:16259881-16259900 None:intergenic 35.0%
! TTATAGAATGTTTTCCAGCG+TGG - chr1_3:16259898-16259917 Msa0106680:intron 35.0%
! TTGGCTGCATTTCTTTTTAC+AGG - chr1_3:16260138-16260157 Msa0106680:intron 35.0%
! CTGCATTTCTTTTTACAGGA+AGG - chr1_3:16260142-16260161 Msa0106680:intron 35.0%
AAAGCACATAACAGAACTCT+AGG - chr1_3:16260719-16260738 Msa0106680:intron 35.0%
! AAGTCTGCTCTTTTACCATT+AGG + chr1_3:16261093-16261112 None:intergenic 35.0%
GTTGTATGATCATAGGATCT+TGG - chr1_3:16261128-16261147 Msa0106680:intron 35.0%
!! CATTTGTTATAGTTTCCTCC+TGG - chr1_3:16261244-16261263 Msa0106680:intron 35.0%
CTCCTTCAGTTTCATTTCTC+TGG - chr1_3:16256506-16256525 Msa0106680:intron 40.0%
TGTCATTGACAATACTGGTC+AGG - chr1_3:16257162-16257181 Msa0106680:intron 40.0%
CACAAGTTTCTCGATCTTGA+TGG - chr1_3:16257420-16257439 Msa0106680:intron 40.0%
CAAGTTTCTCGATCTTGATG+GGG - chr1_3:16257422-16257441 Msa0106680:intron 40.0%
GTAGTCCAATGAGGTAACAT+AGG + chr1_3:16257625-16257644 None:intergenic 40.0%
! AAAGTATTTGGAGTAGACCC+AGG + chr1_3:16257785-16257804 None:intergenic 40.0%
GCATGCTTCTTTCTTAGACT+AGG - chr1_3:16257896-16257915 Msa0106680:intron 40.0%
TCTTTCTTAGACTAGGACAG+AGG - chr1_3:16257903-16257922 Msa0106680:intron 40.0%
CTTTCTTAGACTAGGACAGA+GGG - chr1_3:16257904-16257923 Msa0106680:intron 40.0%
!! CATTCTTTGTTGTTTGGTGC+AGG - chr1_3:16257950-16257969 Msa0106680:intron 40.0%
CTTCAACATGATGCTCTACA+TGG - chr1_3:16257987-16258006 Msa0106680:intron 40.0%
TAAACCCCACATAGTTGTTG+AGG - chr1_3:16258028-16258047 Msa0106680:intron 40.0%
!! ATATTTCTTTAGGGTGGGGT+TGG - chr1_3:16258161-16258180 Msa0106680:intron 40.0%
!! TATTTCTTTAGGGTGGGGTT+GGG - chr1_3:16258162-16258181 Msa0106680:intron 40.0%
TTGGGGTTACCAATTACTAG+TGG - chr1_3:16258180-16258199 Msa0106680:intron 40.0%
TCCCCAGTCATTAAACTTAC+AGG + chr1_3:16258392-16258411 None:intergenic 40.0%
ACTGGGGAATCAAGAGAATT+TGG - chr1_3:16258404-16258423 Msa0106680:intron 40.0%
TTCTGCACCCAAAAATGACA+GGG - chr1_3:16258436-16258455 Msa0106680:intron 40.0%
!!! GTTCCATCCCTGTCATTTTT+GGG + chr1_3:16258446-16258465 None:intergenic 40.0%
GTTCTGCCACTGTTATGTTT+CGG - chr1_3:16258662-16258681 Msa0106680:intron 40.0%
TCGAAACCGAAACATAACAG+TGG + chr1_3:16258671-16258690 None:intergenic 40.0%
TCTAACATGATTACTGCGGA+TGG - chr1_3:16258823-16258842 Msa0106680:CDS 40.0%
TTACAGGCGCTCTTTGATAT+TGG - chr1_3:16259323-16259342 Msa0106680:intron 40.0%
ACTATCTGTTGCAGTCAATG+TGG - chr1_3:16259416-16259435 Msa0106680:CDS 40.0%
CTATCTGTTGCAGTCAATGT+GGG - chr1_3:16259417-16259436 Msa0106680:CDS 40.0%
TGTTGTAACACGATGTAGTG+TGG + chr1_3:16259463-16259482 None:intergenic 40.0%
ATGCAGGGTTGAAAAATGAC+AGG + chr1_3:16260014-16260033 None:intergenic 40.0%
! ACTTGATTGGTTATGCCACA+TGG - chr1_3:16260830-16260849 Msa0106680:intron 40.0%
!! GGCAAGCTAAGTTGATCAAA+AGG - chr1_3:16260851-16260870 Msa0106680:intron 40.0%
! TCTGCTCTTTTACCATTAGG+TGG + chr1_3:16261090-16261109 None:intergenic 40.0%
ATAGCTCTTCACATTCTTGC+TGG + chr1_3:16261354-16261373 None:intergenic 40.0%
! TAAGCTTTCTGAGGAATGTC+TGG - chr1_3:16261429-16261448 Msa0106680:CDS 40.0%
AACGGTGAGAGAATGTTCTG+TGG + chr1_3:16256473-16256492 None:intergenic 45.0%
! GATTACCAGTGCCTTTTGCT+GGG - chr1_3:16256660-16256679 Msa0106680:intron 45.0%
TTTCTCGATCTTGATGGGGA+AGG - chr1_3:16257426-16257445 Msa0106680:intron 45.0%
AAGGAAGAATCCACTCCGTT+TGG - chr1_3:16257445-16257464 Msa0106680:intron 45.0%
!! TGAGGCACCGTGAAATTGTA+AGG + chr1_3:16257475-16257494 None:intergenic 45.0%
TGCATAGCTGTAGTCCAATG+AGG + chr1_3:16257634-16257653 None:intergenic 45.0%
TCATTGGACTACAGCTATGC+AGG - chr1_3:16257633-16257652 Msa0106680:intron 45.0%
GTGAAAGTGAAGCAGATGAC+TGG + chr1_3:16257931-16257950 None:intergenic 45.0%
CTCACCTCAACAACTATGTG+GGG + chr1_3:16258035-16258054 None:intergenic 45.0%
GCTCACCTCAACAACTATGT+GGG + chr1_3:16258036-16258055 None:intergenic 45.0%
!! ATTTCTTTAGGGTGGGGTTG+GGG - chr1_3:16258163-16258182 Msa0106680:intron 45.0%
! TTTTGGTCAGGATGCTGCAA+AGG - chr1_3:16258292-16258311 Msa0106680:intron 45.0%
!!! CATTTTTGGGTGCAGAAGAC+TGG + chr1_3:16258433-16258452 None:intergenic 45.0%
CTTCTGCACCCAAAAATGAC+AGG - chr1_3:16258435-16258454 Msa0106680:intron 45.0%
!!! GGTTCCATCCCTGTCATTTT+TGG + chr1_3:16258447-16258466 None:intergenic 45.0%
AGGAGAATTCTGCCACAGAA+AGG + chr1_3:16258785-16258804 None:intergenic 45.0%
TGAGCTGTAGTTCATGTCTC+TGG + chr1_3:16258947-16258966 None:intergenic 45.0%
! ATCTGGTCTGGCTCAGAATT+TGG - chr1_3:16259009-16259028 Msa0106680:intron 45.0%
! TCTGGTCTGGCTCAGAATTT+GGG - chr1_3:16259010-16259029 Msa0106680:intron 45.0%
GAATTTGGGGACAGTCATCA+AGG - chr1_3:16259024-16259043 Msa0106680:intron 45.0%
ACTCGTAAGACCAACTCGTA+AGG - chr1_3:16259357-16259376 Msa0106680:intron 45.0%
TAAGACCAACTCGTAAGGCA+AGG - chr1_3:16259362-16259381 Msa0106680:intron 45.0%
GGTACATACTAGACCATGAC+TGG - chr1_3:16260237-16260256 Msa0106680:CDS 45.0%
!! GATCAACTTAGCTTGCCATG+TGG + chr1_3:16260848-16260867 None:intergenic 45.0%
TCTTCACATTCTTGCTGGCA+AGG + chr1_3:16261349-16261368 None:intergenic 45.0%
! AACGCTTGCTAAGCTTTCTG+AGG - chr1_3:16261420-16261439 Msa0106680:CDS 45.0%
!!! TAATTTTTTATATAAATATG+TGG - chr1_3:16258623-16258642 Msa0106680:intron 5.0%
!!! CTATTTTATTAAATTATTAT+TGG + chr1_3:16259530-16259549 None:intergenic 5.0%
! GGATTACCAGTGCCTTTTGC+TGG - chr1_3:16256659-16256678 Msa0106680:intron 50.0%
! CAGTGCCTTTTGCTGGGAAT+CGG - chr1_3:16256666-16256685 Msa0106680:intron 50.0%
GTAAGGTCTTCCAAACGGAG+TGG + chr1_3:16257458-16257477 None:intergenic 50.0%
AAGTAAGGAGCAGCTGCAAG+TGG + chr1_3:16257818-16257837 None:intergenic 50.0%
GGTTTATTGGACAGCCTCAC+GGG + chr1_3:16258014-16258033 None:intergenic 50.0%
GGGTTTATTGGACAGCCTCA+CGG + chr1_3:16258015-16258034 None:intergenic 50.0%
GGCTCACCTCAACAACTATG+TGG + chr1_3:16258037-16258056 None:intergenic 50.0%
GCACCCAAAAATGACAGGGA+TGG - chr1_3:16258440-16258459 Msa0106680:intron 50.0%
ACAGGGATGGAACCTCAATG+AGG - chr1_3:16258453-16258472 Msa0106680:intron 50.0%
! CTGGTCTGGCTCAGAATTTG+GGG - chr1_3:16259011-16259030 Msa0106680:intron 50.0%
ACAGTCCTTGCCTTACGAGT+TGG + chr1_3:16259370-16259389 None:intergenic 50.0%
TCCACGATGCCATGCCAAAT+TGG - chr1_3:16259869-16259888 Msa0106680:CDS 50.0%
! GTGCAATGCAGCAGGTTTTG+TGG - chr1_3:16260259-16260278 Msa0106680:intron 50.0%
GCTTAGCAAGCGTTTGTGCT+AGG + chr1_3:16261414-16261433 None:intergenic 50.0%
! CGATTCCCAGCAAAAGGCAC+TGG + chr1_3:16256668-16256687 None:intergenic 55.0%
! TGCCTTTTGCTGGGAATCGG+AGG - chr1_3:16256669-16256688 Msa0106680:intron 55.0%
! GCCTTTTGCTGGGAATCGGA+GGG - chr1_3:16256670-16256689 Msa0106680:intron 55.0%
ACCCTCCGATTCCCAGCAAA+AGG + chr1_3:16256674-16256693 None:intergenic 55.0%
AAGGAGCAGCTGCAAGTGGA+TGG + chr1_3:16257814-16257833 None:intergenic 55.0%
ATGCTCTACATGGTCCCGTG+AGG - chr1_3:16257997-16258016 Msa0106680:intron 55.0%
TGTGTGCACAGTGCAGCAAG+TGG - chr1_3:16258543-16258562 Msa0106680:intron 55.0%
!! GTGATGGGGGCATCTCATGA+CGG + chr1_3:16259734-16259753 None:intergenic 55.0%
GCCAATTTGGCATGGCATCG+TGG + chr1_3:16259873-16259892 None:intergenic 55.0%
ACAAGATCAGTTGGCCACGC+TGG + chr1_3:16259915-16259934 None:intergenic 55.0%
CTGCTGCATTGCACCAGTCA+TGG + chr1_3:16260253-16260272 None:intergenic 55.0%
CATGACTGGTGCAATGCAGC+AGG - chr1_3:16260251-16260270 Msa0106680:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 16256387 16261492 16256387 ID=Msa0106680;Name=Msa0106680
chr1_3 mRNA 16256387 16261492 16256387 ID=Msa0106680-mRNA-1;Parent=Msa0106680;Name=Msa0106680-mRNA-1;_AED=0.18;_eAED=0.18;_QI=0|0.90|0.75|0.91|0.81|0.83|12|0|347
chr1_3 exon 16256387 16256524 16256387 ID=Msa0106680-mRNA-1:exon:6189;Parent=Msa0106680-mRNA-1
chr1_3 exon 16257642 16257719 16257642 ID=Msa0106680-mRNA-1:exon:6188;Parent=Msa0106680-mRNA-1
chr1_3 exon 16258780 16258877 16258780 ID=Msa0106680-mRNA-1:exon:6187;Parent=Msa0106680-mRNA-1
chr1_3 exon 16259035 16259083 16259035 ID=Msa0106680-mRNA-1:exon:6186;Parent=Msa0106680-mRNA-1
chr1_3 exon 16259271 16259325 16259271 ID=Msa0106680-mRNA-1:exon:6185;Parent=Msa0106680-mRNA-1
chr1_3 exon 16259405 16259577 16259405 ID=Msa0106680-mRNA-1:exon:6184;Parent=Msa0106680-mRNA-1
chr1_3 exon 16259830 16259907 16259830 ID=Msa0106680-mRNA-1:exon:6183;Parent=Msa0106680-mRNA-1
chr1_3 exon 16260014 16260109 16260014 ID=Msa0106680-mRNA-1:exon:6182;Parent=Msa0106680-mRNA-1
chr1_3 exon 16260225 16260272 16260225 ID=Msa0106680-mRNA-1:exon:6181;Parent=Msa0106680-mRNA-1
chr1_3 exon 16260378 16260473 16260378 ID=Msa0106680-mRNA-1:exon:6180;Parent=Msa0106680-mRNA-1
chr1_3 exon 16261352 16261452 16261352 ID=Msa0106680-mRNA-1:exon:6179;Parent=Msa0106680-mRNA-1
chr1_3 exon 16261462 16261492 16261462 ID=Msa0106680-mRNA-1:exon:6178;Parent=Msa0106680-mRNA-1
chr1_3 CDS 16261462 16261492 16261462 ID=Msa0106680-mRNA-1:cds;Parent=Msa0106680-mRNA-1
chr1_3 CDS 16261352 16261452 16261352 ID=Msa0106680-mRNA-1:cds;Parent=Msa0106680-mRNA-1
chr1_3 CDS 16260378 16260473 16260378 ID=Msa0106680-mRNA-1:cds;Parent=Msa0106680-mRNA-1
chr1_3 CDS 16260225 16260272 16260225 ID=Msa0106680-mRNA-1:cds;Parent=Msa0106680-mRNA-1
chr1_3 CDS 16260014 16260109 16260014 ID=Msa0106680-mRNA-1:cds;Parent=Msa0106680-mRNA-1
chr1_3 CDS 16259830 16259907 16259830 ID=Msa0106680-mRNA-1:cds;Parent=Msa0106680-mRNA-1
chr1_3 CDS 16259405 16259577 16259405 ID=Msa0106680-mRNA-1:cds;Parent=Msa0106680-mRNA-1
chr1_3 CDS 16259271 16259325 16259271 ID=Msa0106680-mRNA-1:cds;Parent=Msa0106680-mRNA-1
chr1_3 CDS 16259035 16259083 16259035 ID=Msa0106680-mRNA-1:cds;Parent=Msa0106680-mRNA-1
chr1_3 CDS 16258780 16258877 16258780 ID=Msa0106680-mRNA-1:cds;Parent=Msa0106680-mRNA-1
chr1_3 CDS 16257642 16257719 16257642 ID=Msa0106680-mRNA-1:cds;Parent=Msa0106680-mRNA-1
chr1_3 CDS 16256387 16256524 16256387 ID=Msa0106680-mRNA-1:cds;Parent=Msa0106680-mRNA-1
Gene Sequence

>Msa0106680

ATGAATAAAACAGTGTTTGGGCTTTATCGGAACTTTACATTACGCAAACGTGTCTTTCGCGCTCTTTCTCTTCTCCACAGAACATTCTCTCACCGTTTCGTTTTTTCGAACTCCTTCAGTTTCATTTCTCTGGAAGCTACAGGATTCACAAGTTTCTCGATCTTGATGGGGAAGGAAGAATCCACTCCGTTTGGAAGACCTTACAATTTCACGGTGCCTCAAGATGATCAAACTCCAAACAAACCTATGTTACCTCATTGGACTACAGCTATGCAGGCTTATTACAATCCTGGGTCTACTCCAAATACTTTTTATAACCATCCACTTGCAGCTGCTCCTTACTTGTATCATCAGTACATCTTGCAAAATCAGGCAAACAATGCACTGCTTCAACATGATGCTCTACATGGTCCCGTGAGGCTGTCCAATAAACCCCACATAGTTGTTGAGGATGCTGCAAAGGCTTTTTCAGAAATGGTACACAGAAATTTTGGCCAAAAAAACCAAGATTTCAACTTATTTGCTAGAAACTCACAACCTGTAAGTTTAATGACTGGGGAATCAAGAGAATTTGGAAACCAGTCTTCTGCACCCAAAAATGACAGGGATGGAACCTCAATGAGTGCAGCAAGTGGAAGCAAGAGATCACGAGATAGAGATCAAGATGACAGTAACAAAAATTCTCCTTTAACTAAGAAGCATAAATCTAACATGATTACTGCGGATGAAAATTCATCTGGTCTGGCTCAGAATTTGGGGACAGTCATCAAGGAATCTGATGCCGACGTTGTTAATGTCGATGTTGATGCTCAACTTAAAAATATGGAAGGTGATGACATAAGAAAAGAAAGAAAAAGGTTGTCAAACAGGAAATCAGCTAAAAGATCAAAGATAAAGAAACAGCAAGAATGTGAAGAGCTATATAATAAGATAGACACTCTTAAGGATGAAAATTCTGTCCTAGCACAAACGCTTGCTAAGCTTTCTGAGGAATGTCTGGAGCTCGCCAATGAAAATGATTCCATTGAGGTCCATCATTTT
Protein sequence

>Msa0106680

MNKTVFGLYRNFTLRKRVFRALSLLHRTFSHRFVFSNSFSFISLEATGFTSFSILMGKEESTPFGRPYNFTVPQDDQTPNKPMLPHWTTAMQAYYNPGSTPNTFYNHPLAAAPYLYHQYILQNQANNALLQHDALHGPVRLSNKPHIVVEDAAKAFSEMVHRNFGQKNQDFNLFARNSQPVSLMTGESREFGNQSSAPKNDRDGTSMSAASGSKRSRDRDQDDSNKNSPLTKKHKSNMITADENSSGLAQNLGTVIKESDADVVNVDVDAQLKNMEGDDIRKERKRLSNRKSAKRSKIKKQQECEELYNKIDTLKDENSVLAQTLAKLSEECLELANENDSIEVHHF