AlfalfaGEDB | Msa0106760

M. sativa cultivar zhongmu-4 / Msa0106760

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0106760	A0A396JPN4	71.181	288	47	3	171	424	20	305	1.64e-135	406

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TFs/TRs:

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Gene ID	Type	Classification
Msa0106760	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0106760	MtrunA17_Chr1g0163181	71.181	288	47	3	171	424	20	305	3.15e-139	406
Msa0106760	MtrunA17_Chr1g0163201	70.790	291	58	5	159	423	7	296	5.63e-132	386
Msa0106760	MtrunA17_Chr1g0163251	53.818	275	106	3	171	425	20	293	3.78e-94	289
Msa0106760	MtrunA17_Chr1g0163261	44.014	284	130	4	171	430	24	302	1.55e-74	235
Msa0106760	MtrunA17_Chr1g0163231	47.876	259	117	6	171	413	24	280	7.14e-73	231
Msa0106760	MtrunA17_Chr1g0171611	46.125	271	130	6	171	437	24	282	3.79e-71	226
Msa0106760	MtrunA17_Chr1g0163111	80.000	120	21	1	299	418	1	117	5.50e-60	195
Msa0106760	MtrunA17_Chr1g0163151	75.610	123	30	0	299	421	1	123	1.29e-59	194
Msa0106760	MtrunA17_Chr1g0163191	70.714	140	36	1	171	310	20	154	5.95e-58	187
Msa0106760	MtrunA17_Chr1g0163241	78.704	108	22	1	170	276	21	128	3.11e-52	173

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 82 sgRNAs with CRISPR-Local

Find 330 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCGAAACAAGAAGAAAG+AGG	0.544529	1_3:+16415095	Msa0106760:CDS
AAAGAAACCGCTGATTCCGG+CGG	0.670255	1_3:+16415059	Msa0106760:CDS
AAGGGAGAAGGGTGGTGTGT+CGG	0.569235	1_3:+16416538	Msa0106760:CDS
AATATCAAATCAGTTGATGA+AGG	0.540855	1_3:+16418619	Msa0106760:CDS
AATATGGTATGGGTGGTGGA+TGG	0.557585	1_3:+16419527	Msa0106760:CDS
AATCCAGAAATGATTTAACT+CGG	0.634458	1_3:+16414300	Msa0106760:CDS
ACACTGAGAAGGATTTGATT+GGG	0.502843	1_3:+16414330	Msa0106760:CDS
ACCGCTGATTCCGGCGGCAG+CGG	0.388755	1_3:+16415065	Msa0106760:CDS
ACCGCTGGCAGCAATCTCCC+AGG	0.515303	1_3:-16416593	None:intergenic
ACTTACCAAGAAATAGCCAT+TGG	0.604417	1_3:-16419290	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTCTGGATTTCATTCTAACA+GGG	-	chr1_3:16414289-16414308	None:intergenic	30.0%
TTTCTGGATTTCATTCTAAC+AGG	-	chr1_3:16414290-16414309	None:intergenic	30.0%
TTTCCGAGTTAAATCATTTC+TGG	-	chr1_3:16414306-16414325	None:intergenic	30.0%
TTTGATTGGGAAGAAAAATG+TGG	+	chr1_3:16414343-16414362	Msa0106760:CDS	30.0%
TAAATAACAAGCTGAGAATG+TGG	+	chr1_3:16414502-16414521	Msa0106760:intron	30.0%
TTTATTGCCGAAGAAAGAAT+AGG	+	chr1_3:16415508-16415527	Msa0106760:intron	30.0%
CATTGAACCTATTCTTTCTT+CGG	-	chr1_3:16415518-16415537	None:intergenic	30.0%
ATTAGATTGCGTAACCAATT+GGG	-	chr1_3:16415868-16415887	None:intergenic	30.0%
ATTGGACAGAATGAGAAAAT+TGG	+	chr1_3:16415942-16415961	Msa0106760:intron	30.0%
TTTAGGAGGAGATAATCTTT+AGG	+	chr1_3:16416145-16416164	Msa0106760:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	16414254	16419741	16414254	ID=Msa0106760;Name=Msa0106760
chr1_3	mRNA	16414254	16419741	16414254	ID=Msa0106760-mRNA-1;Parent=Msa0106760;Name=Msa0106760-mRNA-1;_AED=0.39;_eAED=0.39;_QI=0\|0.2\|0.16\|0.5\|1\|1\|6\|0\|442
chr1_3	exon	16414254	16414481	16414254	ID=Msa0106760-mRNA-1:exon:6220;Parent=Msa0106760-mRNA-1
chr1_3	exon	16415023	16415192	16415023	ID=Msa0106760-mRNA-1:exon:6221;Parent=Msa0106760-mRNA-1
chr1_3	exon	16416523	16416634	16416523	ID=Msa0106760-mRNA-1:exon:6222;Parent=Msa0106760-mRNA-1
chr1_3	exon	16418370	16418736	16418370	ID=Msa0106760-mRNA-1:exon:6223;Parent=Msa0106760-mRNA-1
chr1_3	exon	16419188	16419306	16419188	ID=Msa0106760-mRNA-1:exon:6224;Parent=Msa0106760-mRNA-1
chr1_3	exon	16419409	16419741	16419409	ID=Msa0106760-mRNA-1:exon:6225;Parent=Msa0106760-mRNA-1
chr1_3	CDS	16414254	16414481	16414254	ID=Msa0106760-mRNA-1:cds;Parent=Msa0106760-mRNA-1
chr1_3	CDS	16415023	16415192	16415023	ID=Msa0106760-mRNA-1:cds;Parent=Msa0106760-mRNA-1
chr1_3	CDS	16416523	16416634	16416523	ID=Msa0106760-mRNA-1:cds;Parent=Msa0106760-mRNA-1
chr1_3	CDS	16418370	16418736	16418370	ID=Msa0106760-mRNA-1:cds;Parent=Msa0106760-mRNA-1
chr1_3	CDS	16419188	16419306	16419188	ID=Msa0106760-mRNA-1:cds;Parent=Msa0106760-mRNA-1
chr1_3	CDS	16419409	16419741	16419409	ID=Msa0106760-mRNA-1:cds;Parent=Msa0106760-mRNA-1

Gene Sequence

>Msa0106760

ATGAAATTAAGCTCTAATAATAAGAATGAAGGCCCTGTTAGAATGAAATCCAGAAATGATTTAACTCGGAAAAAATACACTGAGAAGGATTTGATTGGGAAGAAAAATGTGGAAAATAACTTGTCTGACAAGAAACAGGTTGATGATTCCTTGTCTGGAAAACAACAAACTGGTATTGACTTGTCTGGCAAGAAACAAGTTGTTTTTGAATTGTCTGGTAAGACACAGAGATTCAATGGTGAAGATAGACTTCGATCAGAAGTGAAAGAAACCGCTGATTCCGGCGGCAGCGGAAGAGGAAAACCGAAACAAGAAGAAAGAGGCAGAAATGTCTTCATCTTCATCGAACATACATTTGTTGCTCCGATTGCAGTTCAGCCAAGCGCGGCGGCAATTCGGATGGCTGAGAGAGAAAGGGAGAAGGGTGGTGTGTCGGTGTCAGTTGTGCGCGGTGATGCGCTTGTGGCGCCTGGGAGATTGCTGCCAGCGGTTAGAGGTAGGATGAATGAGAGGGTTCCTAAGATATATGTGGAGAAATATTGGAAAGGAATATCAAACCCTATATTCCTTAAATTTCCAAACGGTGTTGAACAAAAAATATTTTGGGTGGAGAGTAATGGTGATATTTGGTTTCAAAAAAATTGGGAAAGTTTTGCAAAGTCTTTCAAATATGGATATCTCTTAACTTTCAAATTCATTGGTGGATCATATTTTAAGGTTAAGATATTTGGTGCCAATACTTTAGAAATAAACTATTCCAATATCAAATCAGTTGATGAAGGTGCTGAAGACACAGAAGAAGAAAGTCATGAAGAGAGTGATGAATCAAGTGATGATGAAGCCACAGAAGAAAGTCATGAAGAGAGTGATGAATCAAGTAGTAACAAAGTAGCCATGGTGAAGAAGGCTAGAAAATACCTAACATCTGAAGCTGTTAATGAGAATCCCTCTTTTGAAGTTATAATGACTCAATCTTATGCCAATGGCTATTTCTTGTGGATACCAAGTGAGTTTTCAAGAGAACACTTGAAAGATTTTAATGGAACTGCAACAATAAGGGTTGGTAATGAAAGGAGTGTGGAAGTGAGTTTGAGGTATTATGGCACCAAGAAAAAATATGGTATGGGTGGTGGATGGAAAATATTCAGGCAAAAATATAATTTGCAAGTTGATGATCTTTGTAAATTTGAGATGATTCGACGGAGACCAATTTCATTCAATGTTACTATTACTCGTGCCATAAACAAACCAAGCCCAAAGAAATCACTAGGTTTTCTTTCTTTCTTTTTTATTTGGTTTTTTGTATGTTATAATTTGATATTTTATTAA

Protein sequence

>Msa0106760

MKLSSNNKNEGPVRMKSRNDLTRKKYTEKDLIGKKNVENNLSDKKQVDDSLSGKQQTGIDLSGKKQVVFELSGKTQRFNGEDRLRSEVKETADSGGSGRGKPKQEERGRNVFIFIEHTFVAPIAVQPSAAAIRMAEREREKGGVSVSVVRGDALVAPGRLLPAVRGRMNERVPKIYVEKYWKGISNPIFLKFPNGVEQKIFWVESNGDIWFQKNWESFAKSFKYGYLLTFKFIGGSYFKVKIFGANTLEINYSNIKSVDEGAEDTEEESHEESDESSDDEATEESHEESDESSSNKVAMVKKARKYLTSEAVNENPSFEVIMTQSYANGYFLWIPSEFSREHLKDFNGTATIRVGNERSVEVSLRYYGTKKKYGMGGGWKIFRQKYNLQVDDLCKFEMIRRRPISFNVTITRAINKPSPKKSLGFLSFFFIWFFVCYNLIFY*