AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0108120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0108120 MtrunA17_Chr1g0164761 89.161 572 44 1 1 572 1 554 0.0 1059
Msa0108120 MtrunA17_Chr1g0164731 77.622 572 100 3 2 571 39 584 0.0 893
Msa0108120 MtrunA17_Chr5g0410721 52.410 498 230 3 2 493 83 579 0.0 543
Msa0108120 MtrunA17_Chr3g0116441 37.190 363 186 9 8 346 18 362 4.60e-66 234
Msa0108120 MtrunA17_Chr8g0380981 31.455 550 290 15 4 476 39 578 9.87e-58 209
Msa0108120 MtrunA17_Chr7g0214951 80.000 120 6 2 181 299 99 201 1.28e-57 192
Msa0108120 MtrunA17_Chr1g0189211 30.303 528 276 16 6 454 148 662 2.42e-53 196
Msa0108120 MtrunA17_Chr1g0199511 44.681 188 100 1 152 339 291 474 2.07e-52 193
Msa0108120 MtrunA17_Chr5g0397501 32.751 458 222 18 88 511 303 708 6.25e-52 192
Msa0108120 MtrunA17_Chr2g0303101 34.005 397 224 13 134 502 232 618 7.08e-46 174
Msa0108120 MtrunA17_Chr7g0214941 81.081 74 14 0 468 541 1 74 2.76e-31 116
Msa0108120 MtrunA17_Chr7g0261401 31.692 325 178 12 156 455 338 643 3.92e-30 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0108120 AT5G46910.1 54.412 476 212 5 7 479 102 575 0.0 533
Msa0108120 AT5G46910.2 54.412 476 212 5 7 479 102 575 0.0 532
Msa0108120 AT3G48430.1 34.140 372 197 8 8 339 20 383 1.09e-58 212
Msa0108120 AT2G34880.1 31.144 533 285 14 7 470 60 579 1.55e-58 209
Msa0108120 AT1G63490.1 43.396 265 124 6 93 340 52 307 1.49e-56 206
Msa0108120 AT1G63490.4 43.396 265 124 6 93 340 305 560 1.55e-56 206
Msa0108120 AT1G63490.2 43.396 265 124 6 93 340 305 560 1.76e-56 206
Msa0108120 AT1G30810.3 30.112 538 289 13 4 466 55 580 3.37e-56 203
Msa0108120 AT1G30810.2 30.112 538 289 13 4 466 55 580 3.37e-56 203
Msa0108120 AT1G30810.1 30.112 538 289 13 4 466 55 580 3.37e-56 203
Msa0108120 AT4G20400.1 27.183 607 338 17 4 528 52 636 2.66e-53 196
Msa0108120 AT5G04240.1 46.277 188 97 1 152 339 259 442 7.78e-52 192
Msa0108120 AT4G20400.2 27.395 595 328 17 16 528 7 579 1.84e-50 187
Msa0108120 AT1G08620.2 34.483 348 193 8 117 439 313 650 1.44e-46 176
Msa0108120 AT1G08620.4 34.483 348 193 8 117 439 313 650 1.44e-46 176
Msa0108120 AT1G08620.1 34.483 348 193 8 117 439 313 650 1.44e-46 176
Msa0108120 AT1G08620.3 34.483 348 193 8 117 439 327 664 1.55e-46 176
Msa0108120 AT2G38950.1 24.881 631 367 18 2 550 102 707 1.38e-34 139
Msa0108120 AT1G63490.3 55.172 87 39 0 254 340 15 101 6.52e-25 110

Find 76 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACAATTGTACAGCCTCTT+AGG 0.195974 1_3:+19427885 Msa0108120:CDS
TGACATGCAGCCTCTGCTTT+AGG 0.237204 1_3:+19429819 Msa0108120:CDS
TTCTTTACCGCAAAATCTAT+TGG 0.253886 1_3:-19428092 None:intergenic
GCTCCTGAGGCTTCTAAATA+TGG 0.264118 1_3:+19427346 Msa0108120:CDS
TGTCTTAACGGAAGAGAAAA+AGG 0.267528 1_3:+19427850 Msa0108120:CDS
GATGTATTCATTTATGTTAT+AGG 0.277368 1_3:-19430143 None:intergenic
GACATGCAGCCTCTGCTTTA+GGG 0.297262 1_3:+19429820 Msa0108120:CDS
AATCCAGCATGATATGAATT+AGG 0.298533 1_3:-19428981 None:intergenic
AGTTCCTCATATGGAATTAT+AGG 0.302867 1_3:-19429605 None:intergenic
GAGAAAGGAACAGTTGAGTA+TGG 0.324983 1_3:+19428169 Msa0108120:CDS
AGCTATTCAAATACATCATT+AGG 0.343820 1_3:+19429791 Msa0108120:CDS
ATAAAGGACTCGTTGTCTTT+TGG 0.343992 1_3:-19430518 None:intergenic
TTACAAAGAAGTTCCTCATA+TGG 0.344918 1_3:-19429614 None:intergenic
TTACCATGGAAAAGGCAAAT+TGG 0.359872 1_3:-19429887 None:intergenic
AACAAGCAATTTCAATTTGA+AGG 0.380018 1_3:+19428243 Msa0108120:CDS
TAAACTTTGCTATTGGTGAT+TGG 0.380389 1_3:+19429531 Msa0108120:CDS
GACAAAAGAAGCAGGATTAG+AGG 0.392437 1_3:-19427831 None:intergenic
TTTGATTTGAAAACCCTAAG+AGG 0.423006 1_3:-19427899 None:intergenic
ACTGAAAGCAGAATAATAAC+TGG 0.424878 1_3:+19430338 Msa0108120:intron
TCAATTTGAAAAGACTGTCT+TGG 0.425142 1_3:+19428797 Msa0108120:CDS
GACAACTTACTGTATTTAAG+AGG 0.425768 1_3:+19430032 Msa0108120:CDS
TGTTCTGATGGATAATAAGT+TGG 0.426588 1_3:-19427286 None:intergenic
CAGATCCTATGCTTTACATT+GGG 0.427121 1_3:+19428574 Msa0108120:CDS
CACAATTGTACAGCCTCTTA+GGG 0.435355 1_3:+19427886 Msa0108120:CDS
GCTTCCTATAATTCCATATG+AGG 0.437753 1_3:+19429601 Msa0108120:CDS
CTTGTTCCAAGCTTATCATC+AGG 0.440827 1_3:-19428226 None:intergenic
TTGGTGATTGGTTTCCACTC+GGG 0.449709 1_3:+19429543 Msa0108120:CDS
GAAGCTGTAAACTTTGCTAT+TGG 0.453482 1_3:+19429524 Msa0108120:CDS
TTTCTGTCAAGTAAACGTAA+TGG 0.468536 1_3:-19428452 None:intergenic
TGCTCAAACTCTTGTTCTGA+TGG 0.476367 1_3:-19427298 None:intergenic
TTACCATATTTAGAAGCCTC+AGG 0.481047 1_3:-19427349 None:intergenic
TTTCCTAATTCATATCATGC+TGG 0.481919 1_3:+19428978 Msa0108120:CDS
ATGAGGTCTTACCATGGAAA+AGG 0.484485 1_3:-19429895 None:intergenic
ACATAATGTGCCGGTTTACA+AGG 0.487938 1_3:+19428923 Msa0108120:CDS
TTGAAGATCACTATTTGTAC+AGG 0.488775 1_3:+19428625 Msa0108120:CDS
GTGCCACAAAATTGTTTATG+TGG 0.488853 1_3:+19430007 Msa0108120:CDS
ACAGATCCTATGCTTTACAT+TGG 0.492728 1_3:+19428573 Msa0108120:CDS
ATTTGAAGCTCTTGCAAACA+AGG 0.496352 1_3:+19428063 Msa0108120:CDS
GAAATGATACATGGAGAGAA+AGG 0.501243 1_3:+19428154 Msa0108120:CDS
ATCATCAGGATCACTTGAAA+AGG 0.501772 1_3:-19428212 None:intergenic
AATTGAGAGGCTGCAGAGCT+AGG 0.502653 1_3:-19428780 None:intergenic
ATTGGTGATTGGTTTCCACT+CGG 0.503752 1_3:+19429542 Msa0108120:CDS
TACAACTCCTCAAAAGATAG+AGG 0.507371 1_3:+19429653 Msa0108120:CDS
GGTGGAAATAAAACTTGGTA+TGG 0.514584 1_3:+19428753 Msa0108120:CDS
AGTGATCCTGATGATAAGCT+TGG 0.516084 1_3:+19428220 Msa0108120:CDS
TTAACAGAACATGGAGAGAA+TGG 0.517335 1_3:+19428843 Msa0108120:CDS
TGCTATTCAGTATGTTTGCA+TGG 0.518171 1_3:+19428598 Msa0108120:CDS
TGGTGATTGGTTTCCACTCG+GGG 0.519820 1_3:+19429544 Msa0108120:CDS
TTACAAGGCGATGCAAAAGC+CGG 0.522105 1_3:+19428938 Msa0108120:CDS
TCCGTTAAGACAAAAGAAGC+AGG 0.523048 1_3:-19427839 None:intergenic
ACTCAGGTGGAAATAAAACT+TGG 0.530514 1_3:+19428748 Msa0108120:CDS
TTATGTTCTAACAAGACATC+AGG 0.538881 1_3:-19428906 None:intergenic
AGAAGAACTGTTAACTCGCT+AGG 0.543521 1_3:+19430379 Msa0108120:CDS
CTTGTTAGAACATAATGTGC+CGG 0.547471 1_3:+19428914 Msa0108120:CDS
TCTGCAGAAAATTGCTCCTG+AGG 0.560467 1_3:+19427333 Msa0108120:CDS
TTTATAAGACATCATTATCA+CGG 0.560917 1_3:+19429744 Msa0108120:CDS
CAAGACATCAGGAGAGAACA+TGG 0.563327 1_3:-19428895 None:intergenic
TACAAGGCGATGCAAAAGCC+GGG 0.563332 1_3:+19428939 Msa0108120:CDS
ATAAATTATCATCACTCAGG+TGG 0.565750 1_3:+19428735 Msa0108120:CDS
AATGGAGACTGTGGCAGCCG+CGG 0.576119 1_3:-19428434 None:intergenic
TGCCACAAAATTGTTTATGT+GGG 0.576308 1_3:+19430008 Msa0108120:CDS
TTCTGGAACGAAATGATACA+TGG 0.576931 1_3:+19428145 Msa0108120:CDS
GCAGGATTAGAGGCATCGAT+TGG 0.582539 1_3:-19427821 None:intergenic
AAGGTGATGACAAACTCTCC+CGG 0.582876 1_3:-19428957 None:intergenic
AGCTAAAGGGAAGATACTGC+AGG 0.589749 1_3:-19430580 None:intergenic
AGTATAAATTATCATCACTC+AGG 0.598943 1_3:+19428732 Msa0108120:intron
AACAAAATCTTAACAGAACA+TGG 0.599486 1_3:+19428834 Msa0108120:CDS
AGCATCCCAATGTAAAGCAT+AGG 0.618659 1_3:-19428579 None:intergenic
TCGCTAGCTGCAGCCCCGAG+TGG 0.621053 1_3:-19429557 None:intergenic
TATCATGCTGGATTTAGTCA+CGG 0.625895 1_3:+19428990 Msa0108120:CDS
AGCATGATATGAATTAGGAA+AGG 0.636839 1_3:-19428976 None:intergenic
GCAGAATAATAACTGGCATG+AGG 0.639560 1_3:+19430345 Msa0108120:intron
CATAACAATCCCTAAAGCAG+AGG 0.654524 1_3:-19429829 None:intergenic
TGAATACATCAAAGAACACA+AGG 0.678690 1_3:+19430156 Msa0108120:CDS
AGTAAACGTAATGGAGACTG+TGG 0.682153 1_3:-19428443 None:intergenic
TGAGTATGGAGTCAATGTTG+AGG 0.703101 1_3:+19428183 Msa0108120:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAGTATAACCTATATTT+TGG - chr1_3:19427644-19427663 None:intergenic 15.0%
!! ATATTTGATCTTAATATTTC+AGG + chr1_3:19427779-19427798 Msa0108120:intron 15.0%
!! TGATGAAAAATTAACTAATT+TGG + chr1_3:19428000-19428019 Msa0108120:intron 15.0%
!! AATTTATACTGCAAAAAATA+CGG - chr1_3:19428722-19428741 None:intergenic 15.0%
!!! AACAACTTTTAATTCAGATT+AGG - chr1_3:19428506-19428525 None:intergenic 20.0%
!!! TGGATATTGCTTATATTTTA+CGG + chr1_3:19429079-19429098 Msa0108120:intron 20.0%
!!! ACATAAGTATTTAAAATAGC+AGG - chr1_3:19429401-19429420 None:intergenic 20.0%
!!! ATCATTTTCATGTTGATTTT+AGG + chr1_3:19429488-19429507 Msa0108120:intron 20.0%
!! TTTATAAGACATCATTATCA+CGG + chr1_3:19429744-19429763 Msa0108120:CDS 20.0%
!! AATAGTCAAATAGAATAATG+AGG - chr1_3:19429915-19429934 None:intergenic 20.0%
!! GATGTATTCATTTATGTTAT+AGG - chr1_3:19430146-19430165 None:intergenic 20.0%
!! ATTTTCTGCAGATAAACTAA+AGG - chr1_3:19427325-19427344 None:intergenic 25.0%
! ATAAAAAAACCCTAGAAACA+TGG - chr1_3:19427407-19427426 None:intergenic 25.0%
! ATAAAAAAACCCTAGAAACA+CGG - chr1_3:19427407-19427426 None:intergenic 25.0%
! TAGCATTCTTTACTATAGTT+GGG - chr1_3:19427525-19427544 None:intergenic 25.0%
! AAAGTGAAACTCCAAAATAT+AGG + chr1_3:19427630-19427649 Msa0108120:intron 25.0%
! AGAACGATGTAATCTAATAT+AGG + chr1_3:19427689-19427708 Msa0108120:intron 25.0%
!!! TAACTTTTTCTAAGAGAAGA+AGG + chr1_3:19427936-19427955 Msa0108120:CDS 25.0%
! AACAAGCAATTTCAATTTGA+AGG + chr1_3:19428243-19428262 Msa0108120:CDS 25.0%
! AGTCTATTAATCTTTACTTG+TGG + chr1_3:19428348-19428367 Msa0108120:intron 25.0%
!! TACAAAAATTGATTCCTTAC+TGG - chr1_3:19428482-19428501 None:intergenic 25.0%
! AGTATAAATTATCATCACTC+AGG + chr1_3:19428732-19428751 Msa0108120:intron 25.0%
! AACAAAATCTTAACAGAACA+TGG + chr1_3:19428834-19428853 Msa0108120:CDS 25.0%
!!! TTGTTTTTTGTGCAAGAAAT+TGG - chr1_3:19428875-19428894 None:intergenic 25.0%
!! CTTATATTTTACGGATAAGT+AGG + chr1_3:19429088-19429107 Msa0108120:intron 25.0%
! GATTACTTGTGTAAATACTA+CGG - chr1_3:19429172-19429191 None:intergenic 25.0%
! CGATATTTAATTATGGAGTT+CGG - chr1_3:19429262-19429281 None:intergenic 25.0%
!!! TTGATTTTAGGTTTTAACTG+TGG + chr1_3:19429500-19429519 Msa0108120:intron 25.0%
! AGCTATTCAAATACATCATT+AGG + chr1_3:19429791-19429810 Msa0108120:CDS 25.0%
!!! TAATTTGCTTGTGTTATTGA+TGG + chr1_3:19429950-19429969 Msa0108120:intron 25.0%
!! ATGGATAAACTTGCTTATTA+TGG + chr1_3:19429969-19429988 Msa0108120:intron 25.0%
!!! ACTATTTTAATGAAGTTCAG+AGG - chr1_3:19430225-19430244 None:intergenic 25.0%
!! CATGTTTTCTCAAGTTATAA+AGG - chr1_3:19430537-19430556 None:intergenic 25.0%
! TGTTCTGATGGATAATAAGT+TGG - chr1_3:19427289-19427308 None:intergenic 30.0%
GTAGCATTCTTTACTATAGT+TGG - chr1_3:19427526-19427545 None:intergenic 30.0%
!! GAATGCTACTTACTTTCTTT+TGG + chr1_3:19427537-19427556 Msa0108120:intron 30.0%
!! TTTGATTTGAAAACCCTAAG+AGG - chr1_3:19427902-19427921 None:intergenic 30.0%
! GGTTTTCAAATCAAATGAGA+TGG + chr1_3:19427907-19427926 Msa0108120:CDS 30.0%
TTCTTTACCGCAAAATCTAT+TGG - chr1_3:19428095-19428114 None:intergenic 30.0%
!! TAGATATCGAAAAAGTGTTC+TGG + chr1_3:19428128-19428147 Msa0108120:CDS 30.0%
!!! TTTGATTTTAGAATTTGCCG+CGG + chr1_3:19428417-19428436 Msa0108120:intron 30.0%
TTTCTGTCAAGTAAACGTAA+TGG - chr1_3:19428455-19428474 None:intergenic 30.0%
TTGACAGAAAAATTCCAGTA+AGG + chr1_3:19428465-19428484 Msa0108120:intron 30.0%
TTGAAGATCACTATTTGTAC+AGG + chr1_3:19428625-19428644 Msa0108120:CDS 30.0%
ATAAATTATCATCACTCAGG+TGG + chr1_3:19428735-19428754 Msa0108120:CDS 30.0%
TCAATTTGAAAAGACTGTCT+TGG + chr1_3:19428797-19428816 Msa0108120:CDS 30.0%
TTATGTTCTAACAAGACATC+AGG - chr1_3:19428909-19428928 None:intergenic 30.0%
AGCATGATATGAATTAGGAA+AGG - chr1_3:19428979-19428998 None:intergenic 30.0%
TTTCCTAATTCATATCATGC+TGG + chr1_3:19428978-19428997 Msa0108120:CDS 30.0%
AATCCAGCATGATATGAATT+AGG - chr1_3:19428984-19429003 None:intergenic 30.0%
TTCTCTCTCTATAAATGTGT+TGG + chr1_3:19429039-19429058 Msa0108120:intron 30.0%
!! TGGTGTTATTATGTTTCTGA+TGG + chr1_3:19429059-19429078 Msa0108120:intron 30.0%
AGGTCGATTGAATAATTGAA+AGG + chr1_3:19429112-19429131 Msa0108120:intron 30.0%
! TGAATAATTGAAAGGTACGT+AGG + chr1_3:19429120-19429139 Msa0108120:intron 30.0%
CTTTCTATTATGCAAGCTTA+GGG - chr1_3:19429201-19429220 None:intergenic 30.0%
GAACACGCGATATTTAATTA+TGG - chr1_3:19429269-19429288 None:intergenic 30.0%
!! TAAACTTTGCTATTGGTGAT+TGG + chr1_3:19429531-19429550 Msa0108120:CDS 30.0%
AGTTCCTCATATGGAATTAT+AGG - chr1_3:19429608-19429627 None:intergenic 30.0%
TTACAAAGAAGTTCCTCATA+TGG - chr1_3:19429617-19429636 None:intergenic 30.0%
! TTTGTAACCTCTATCTTTTG+AGG - chr1_3:19429663-19429682 None:intergenic 30.0%
TGCCACAAAATTGTTTATGT+GGG + chr1_3:19430008-19430027 Msa0108120:CDS 30.0%
GACAACTTACTGTATTTAAG+AGG + chr1_3:19430032-19430051 Msa0108120:CDS 30.0%
! CTTTTCCAGTTGAAAACTTT+TGG - chr1_3:19430086-19430105 None:intergenic 30.0%
! CAAAAGTTTTCAACTGGAAA+AGG + chr1_3:19430084-19430103 Msa0108120:CDS 30.0%
TGAATACATCAAAGAACACA+AGG + chr1_3:19430156-19430175 Msa0108120:CDS 30.0%
ACTGAAAGCAGAATAATAAC+TGG + chr1_3:19430338-19430357 Msa0108120:intron 30.0%
TTACCATATTTAGAAGCCTC+AGG - chr1_3:19427352-19427371 None:intergenic 35.0%
! TTTTCCATCACCATGTTTCT+AGG + chr1_3:19427394-19427413 Msa0108120:intron 35.0%
! TTTCCATCACCATGTTTCTA+GGG + chr1_3:19427395-19427414 Msa0108120:intron 35.0%
AGGTTATACTAAAACCACAG+TGG + chr1_3:19427650-19427669 Msa0108120:intron 35.0%
ACAACAGATTCTGCATTTGT+AGG + chr1_3:19427724-19427743 Msa0108120:intron 35.0%
! TCCTGCTTCTTTTGTCTTAA+CGG + chr1_3:19427838-19427857 Msa0108120:CDS 35.0%
TGTCTTAACGGAAGAGAAAA+AGG + chr1_3:19427850-19427869 Msa0108120:CDS 35.0%
! ATTTGAAGCTCTTGCAAACA+AGG + chr1_3:19428063-19428082 Msa0108120:CDS 35.0%
! GCGTTTTCCAATAGATTTTG+CGG + chr1_3:19428085-19428104 Msa0108120:CDS 35.0%
!! TTCTGGAACGAAATGATACA+TGG + chr1_3:19428145-19428164 Msa0108120:CDS 35.0%
GAAATGATACATGGAGAGAA+AGG + chr1_3:19428154-19428173 Msa0108120:CDS 35.0%
ATCATCAGGATCACTTGAAA+AGG - chr1_3:19428215-19428234 None:intergenic 35.0%
ACAGATCCTATGCTTTACAT+TGG + chr1_3:19428573-19428592 Msa0108120:CDS 35.0%
CAGATCCTATGCTTTACATT+GGG + chr1_3:19428574-19428593 Msa0108120:CDS 35.0%
TGCTATTCAGTATGTTTGCA+TGG + chr1_3:19428598-19428617 Msa0108120:CDS 35.0%
ACTCAGGTGGAAATAAAACT+TGG + chr1_3:19428748-19428767 Msa0108120:CDS 35.0%
GGTGGAAATAAAACTTGGTA+TGG + chr1_3:19428753-19428772 Msa0108120:CDS 35.0%
! GACAGTCTTTTCAAATTGAG+AGG - chr1_3:19428796-19428815 None:intergenic 35.0%
TTAACAGAACATGGAGAGAA+TGG + chr1_3:19428843-19428862 Msa0108120:CDS 35.0%
CTTGTTAGAACATAATGTGC+CGG + chr1_3:19428914-19428933 Msa0108120:CDS 35.0%
TATCATGCTGGATTTAGTCA+CGG + chr1_3:19428990-19429009 Msa0108120:CDS 35.0%
! TATTTTACGGATAAGTAGGC+AGG + chr1_3:19429092-19429111 Msa0108120:intron 35.0%
CCTTTCTATTATGCAAGCTT+AGG - chr1_3:19429202-19429221 None:intergenic 35.0%
CCTAAGCTTGCATAATAGAA+AGG + chr1_3:19429199-19429218 Msa0108120:intron 35.0%
AACTATTAGAACTTCAGGCA+TGG - chr1_3:19429315-19429334 None:intergenic 35.0%
GCAGTAACTATTAGAACTTC+AGG - chr1_3:19429320-19429339 None:intergenic 35.0%
! GAAGCTGTAAACTTTGCTAT+TGG + chr1_3:19429524-19429543 Msa0108120:CDS 35.0%
GCTTCCTATAATTCCATATG+AGG + chr1_3:19429601-19429620 Msa0108120:CDS 35.0%
TACAACTCCTCAAAAGATAG+AGG + chr1_3:19429653-19429672 Msa0108120:CDS 35.0%
TTACCATGGAAAAGGCAAAT+TGG - chr1_3:19429890-19429909 None:intergenic 35.0%
AGAATAATGAGGTCTTACCA+TGG - chr1_3:19429904-19429923 None:intergenic 35.0%
GTGCCACAAAATTGTTTATG+TGG + chr1_3:19430007-19430026 Msa0108120:CDS 35.0%
! CTCCCACATAAACAATTTTG+TGG - chr1_3:19430013-19430032 None:intergenic 35.0%
GTATGCATTGCTTTATCTTC+AGG + chr1_3:19430269-19430288 Msa0108120:intron 35.0%
! ATAAAGGACTCGTTGTCTTT+TGG - chr1_3:19430521-19430540 None:intergenic 35.0%
TGCTCAAACTCTTGTTCTGA+TGG - chr1_3:19427301-19427320 None:intergenic 40.0%
AAACCCTAGAAACATGGTGA+TGG - chr1_3:19427401-19427420 None:intergenic 40.0%
AACATCATCACCGTGTTTCT+AGG + chr1_3:19427449-19427468 Msa0108120:intron 40.0%
! ACATCATCACCGTGTTTCTA+GGG + chr1_3:19427450-19427469 Msa0108120:intron 40.0%
CGCGTGCATGTTTAGATTAA+AGG + chr1_3:19427584-19427603 Msa0108120:intron 40.0%
GACAAAAGAAGCAGGATTAG+AGG - chr1_3:19427834-19427853 None:intergenic 40.0%
TCCGTTAAGACAAAAGAAGC+AGG - chr1_3:19427842-19427861 None:intergenic 40.0%
ACACAATTGTACAGCCTCTT+AGG + chr1_3:19427885-19427904 Msa0108120:CDS 40.0%
CACAATTGTACAGCCTCTTA+GGG + chr1_3:19427886-19427905 Msa0108120:CDS 40.0%
GAGAAAGGAACAGTTGAGTA+TGG + chr1_3:19428169-19428188 Msa0108120:CDS 40.0%
TGAGTATGGAGTCAATGTTG+AGG + chr1_3:19428183-19428202 Msa0108120:CDS 40.0%
AGTGATCCTGATGATAAGCT+TGG + chr1_3:19428220-19428239 Msa0108120:CDS 40.0%
CTTGTTCCAAGCTTATCATC+AGG - chr1_3:19428229-19428248 None:intergenic 40.0%
! AGTAAACGTAATGGAGACTG+TGG - chr1_3:19428446-19428465 None:intergenic 40.0%
AGCATCCCAATGTAAAGCAT+AGG - chr1_3:19428582-19428601 None:intergenic 40.0%
!! ACATAATGTGCCGGTTTACA+AGG + chr1_3:19428923-19428942 Msa0108120:CDS 40.0%
! TTTTGCATCGCCTTGTAAAC+CGG - chr1_3:19428936-19428955 None:intergenic 40.0%
! TGAAAGGTACGTAGGTTTCA+AGG + chr1_3:19429128-19429147 Msa0108120:intron 40.0%
CAGGAACTGAAATTCTGCAT+GGG - chr1_3:19429382-19429401 None:intergenic 40.0%
!! ATTGGTGATTGGTTTCCACT+CGG + chr1_3:19429542-19429561 Msa0108120:CDS 40.0%
CATAACAATCCCTAAAGCAG+AGG - chr1_3:19429832-19429851 None:intergenic 40.0%
! GATCCAATTTGCCTTTTCCA+TGG + chr1_3:19429884-19429903 Msa0108120:CDS 40.0%
ATGAGGTCTTACCATGGAAA+AGG - chr1_3:19429898-19429917 None:intergenic 40.0%
! TGCTGCCAAAAGTTTTCAAC+TGG + chr1_3:19430078-19430097 Msa0108120:CDS 40.0%
GCAGAATAATAACTGGCATG+AGG + chr1_3:19430345-19430364 Msa0108120:intron 40.0%
AGAAGAACTGTTAACTCGCT+AGG + chr1_3:19430379-19430398 Msa0108120:CDS 40.0%
! CTTTTGGTACTCATCACTGA+TGG - chr1_3:19430505-19430524 None:intergenic 40.0%
TCTGCAGAAAATTGCTCCTG+AGG + chr1_3:19427333-19427352 Msa0108120:CDS 45.0%
GCTCCTGAGGCTTCTAAATA+TGG + chr1_3:19427346-19427365 Msa0108120:CDS 45.0%
GAACTTGAGTACTTCAGCGA+TGG - chr1_3:19427565-19427584 None:intergenic 45.0%
CAAGACATCAGGAGAGAACA+TGG - chr1_3:19428898-19428917 None:intergenic 45.0%
TTACAAGGCGATGCAAAAGC+CGG + chr1_3:19428938-19428957 Msa0108120:CDS 45.0%
AAGGTGATGACAAACTCTCC+CGG - chr1_3:19428960-19428979 None:intergenic 45.0%
GCAGGAACTGAAATTCTGCA+TGG - chr1_3:19429383-19429402 None:intergenic 45.0%
!! TTGGTGATTGGTTTCCACTC+GGG + chr1_3:19429543-19429562 Msa0108120:CDS 45.0%
!!! TTAATTTTATTCAAAATTTT+AGG + chr1_3:19428545-19428564 Msa0108120:intron 5.0%
!!! TAATTTTATTCAAAATTTTA+GGG + chr1_3:19428546-19428565 Msa0108120:intron 5.0%
! CAAATCATAGCGTGCCACTG+TGG - chr1_3:19427667-19427686 None:intergenic 50.0%
!! GCAGGATTAGAGGCATCGAT+TGG - chr1_3:19427824-19427843 None:intergenic 50.0%
AATTGAGAGGCTGCAGAGCT+AGG - chr1_3:19428783-19428802 None:intergenic 50.0%
TACAAGGCGATGCAAAAGCC+GGG + chr1_3:19428939-19428958 Msa0108120:CDS 50.0%
GTAATTCCGCCACTATGCAG+CGG + chr1_3:19429221-19429240 Msa0108120:intron 50.0%
!! TGGTGATTGGTTTCCACTCG+GGG + chr1_3:19429544-19429563 Msa0108120:CDS 50.0%
TGACATGCAGCCTCTGCTTT+AGG + chr1_3:19429819-19429838 Msa0108120:CDS 50.0%
GACATGCAGCCTCTGCTTTA+GGG + chr1_3:19429820-19429839 Msa0108120:CDS 50.0%
TGCAGCGGCGTAGGAACAAT+AGG + chr1_3:19429236-19429255 Msa0108120:intron 55.0%
AATGGAGACTGTGGCAGCCG+CGG - chr1_3:19428437-19428456 None:intergenic 60.0%
GTTCCTACGCCGCTGCATAG+TGG - chr1_3:19429233-19429252 None:intergenic 60.0%
CCTACGCCGCTGCATAGTGG+CGG - chr1_3:19429230-19429249 None:intergenic 65.0%
CCGCCACTATGCAGCGGCGT+AGG + chr1_3:19429227-19429246 Msa0108120:intron 70.0%
TCGCTAGCTGCAGCCCCGAG+TGG - chr1_3:19429560-19429579 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 19427262 19430600 19427262 ID=Msa0108120;Name=Msa0108120
chr1_3 mRNA 19427262 19430600 19427262 ID=Msa0108120-mRNA-1;Parent=Msa0108120;Name=Msa0108120-mRNA-1;_AED=0.32;_eAED=0.32;_QI=0|0|0|1|1|1|9|0|572
chr1_3 exon 19427262 19427367 19427262 ID=Msa0108120-mRNA-1:exon:6996;Parent=Msa0108120-mRNA-1
chr1_3 exon 19427801 19427957 19427801 ID=Msa0108120-mRNA-1:exon:6997;Parent=Msa0108120-mRNA-1
chr1_3 exon 19428045 19428264 19428045 ID=Msa0108120-mRNA-1:exon:6998;Parent=Msa0108120-mRNA-1
chr1_3 exon 19428428 19428481 19428428 ID=Msa0108120-mRNA-1:exon:6999;Parent=Msa0108120-mRNA-1
chr1_3 exon 19428567 19428646 19428567 ID=Msa0108120-mRNA-1:exon:7000;Parent=Msa0108120-mRNA-1
chr1_3 exon 19428734 19429011 19428734 ID=Msa0108120-mRNA-1:exon:7001;Parent=Msa0108120-mRNA-1
chr1_3 exon 19429510 19429905 19429510 ID=Msa0108120-mRNA-1:exon:7002;Parent=Msa0108120-mRNA-1
chr1_3 exon 19430002 19430177 19430002 ID=Msa0108120-mRNA-1:exon:7003;Parent=Msa0108120-mRNA-1
chr1_3 exon 19430349 19430600 19430349 ID=Msa0108120-mRNA-1:exon:7004;Parent=Msa0108120-mRNA-1
chr1_3 CDS 19427262 19427367 19427262 ID=Msa0108120-mRNA-1:cds;Parent=Msa0108120-mRNA-1
chr1_3 CDS 19427801 19427957 19427801 ID=Msa0108120-mRNA-1:cds;Parent=Msa0108120-mRNA-1
chr1_3 CDS 19428045 19428264 19428045 ID=Msa0108120-mRNA-1:cds;Parent=Msa0108120-mRNA-1
chr1_3 CDS 19428428 19428481 19428428 ID=Msa0108120-mRNA-1:cds;Parent=Msa0108120-mRNA-1
chr1_3 CDS 19428567 19428646 19428567 ID=Msa0108120-mRNA-1:cds;Parent=Msa0108120-mRNA-1
chr1_3 CDS 19428734 19429011 19428734 ID=Msa0108120-mRNA-1:cds;Parent=Msa0108120-mRNA-1
chr1_3 CDS 19429510 19429905 19429510 ID=Msa0108120-mRNA-1:cds;Parent=Msa0108120-mRNA-1
chr1_3 CDS 19430002 19430177 19430002 ID=Msa0108120-mRNA-1:cds;Parent=Msa0108120-mRNA-1
chr1_3 CDS 19430349 19430600 19430349 ID=Msa0108120-mRNA-1:cds;Parent=Msa0108120-mRNA-1
Gene Sequence

>Msa0108120

ATGACGAACATTACTCAAGAATGTCCAACTTATTATCCATCAGAACAAGAGTTTGAGCATCCTTTAGTTTATCTGCAGAAAATTGCTCCTGAGGCTTCTAAATATGGTATGTGCAAAATTGTTTCACCAATCGATGCCTCTAATCCTGCTTCTTTTGTCTTAACGGAAGAGAAAAAGGATTTTAAGTTTAACACAATTGTACAGCCTCTTAGGGTTTTCAAATCAAATGAGATGGATAAAATAACTTTTTCTAAGAGAAGAAGAAAATATACATATCATGAATTTGAAGCTCTTGCAAACAAGGCGTTTTCCAATAGATTTTGCGGTAAAGAAGATCTCTCTTGTTTAGATATCGAAAAAGTGTTCTGGAACGAAATGATACATGGAGAGAAAGGAACAGTTGAGTATGGAGTCAATGTTGAGGAAAGCGCCTTTTCAAGTGATCCTGATGATAAGCTTGGAACAAGCAATTTCAATTTGAAGAATTTGCCGCGGCTGCCACAGTCTCCATTACGTTTACTTGACAGAAAAATTCCAGGATTAACAGATCCTATGCTTTACATTGGGATGCTATTCAGTATGTTTGCATGGCATGTTGAAGATCACTATTTGTACAGTATAAATTATCATCACTCAGGTGGAAATAAAACTTGGTATGGCGTGCCTAGCTCTGCAGCCTCTCAATTTGAAAAGACTGTCTTGGATCATGTGTATTGCAACAAAATCTTAACAGAACATGGAGAGAATGGTGCATTCCAATTTCTTGCACAAAAAACAACCATGTTCTCTCCTGATGTCTTGTTAGAACATAATGTGCCGGTTTACAAGGCGATGCAAAAGCCGGGAGAGTTTGTCATCACCTTTCCTAATTCATATCATGCTGGATTTAGTCACGGTTTTAACTGTGGAGAAGCTGTAAACTTTGCTATTGGTGATTGGTTTCCACTCGGGGCTGCAGCTAGCGAACGTTATTCGCATCTCAAAATGCTTCCTATAATTCCATATGAGGAACTTCTTTGTAAAGAAGCATTGCTTATCTACAACTCCTCAAAAGATAGAGGTTACAAAATCAAACATGAAGACACATCTTATCGAGCTATTGTACTGTCTTTCTTGCATCTTATTCAATTTTATAAGACATCATTATCACGGTTCGACAGTTCGATAAAGCTTTCAAGCTATTCAAATACATCATTAGGTTCTGTGACATGCAGCCTCTGCTTTAGGGATTGTTATGTAGCTTTCTTGTTGTGCAAGAAATGCTATTCAGATCCAATTTGCCTTTTCCATGACATTGTGCCACAAAATTGTTTATGTGGGAGACAACTTACTGTATTTAAGAGGAATGACATGTTAGAACTCGAAGATGCTGCCAAAAGTTTTCAACTGGAAAAGGAATGCATCGATGCATTATCTTTAACAAATTTATTCTCCTATAACATAAATGAATACATCAAAGAACACAAGAATAATAACTGGCATGAGGAAAAAAGTGCTAGAAGAACTGTTAACTCGCTAGGCGCAACATCTAAAATGAAAACAAGAGTTGCGAACAACATCAAGCACACTGTTGAAAATAATCAAAAGAAGACAAAGAATCGACGTAGAAACAATGCGTCTCCATCAGTGATGAGTACCAAAAGACAACGAGTCCTTTATAACTTGAGAAAACATGAATCTGAACTGATTAACAAAGTTCCTGCAGTATCTTCCCTTTAG
Protein sequence

>Msa0108120

MTNITQECPTYYPSEQEFEHPLVYLQKIAPEASKYGMCKIVSPIDASNPASFVLTEEKKDFKFNTIVQPLRVFKSNEMDKITFSKRRRKYTYHEFEALANKAFSNRFCGKEDLSCLDIEKVFWNEMIHGEKGTVEYGVNVEESAFSSDPDDKLGTSNFNLKNLPRLPQSPLRLLDRKIPGLTDPMLYIGMLFSMFAWHVEDHYLYSINYHHSGGNKTWYGVPSSAASQFEKTVLDHVYCNKILTEHGENGAFQFLAQKTTMFSPDVLLEHNVPVYKAMQKPGEFVITFPNSYHAGFSHGFNCGEAVNFAIGDWFPLGAAASERYSHLKMLPIIPYEELLCKEALLIYNSSKDRGYKIKHEDTSYRAIVLSFLHLIQFYKTSLSRFDSSIKLSSYSNTSLGSVTCSLCFRDCYVAFLLCKKCYSDPICLFHDIVPQNCLCGRQLTVFKRNDMLELEDAAKSFQLEKECIDALSLTNLFSYNINEYIKEHKNNNWHEEKSARRTVNSLGATSKMKTRVANNIKHTVENNQKKTKNRRRNNASPSVMSTKRQRVLYNLRKHESELINKVPAVSSL*