Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0110540 | AES60398.2 | 85.821 | 134 | 19 | 0 | 1 | 134 | 1 | 134 | 5.33e-73 | 224 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0110540 | G7I6L4 | 85.821 | 134 | 19 | 0 | 1 | 134 | 1 | 134 | 2.55e-73 | 224 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0110540 | TF | C2C2-GATA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0110540 | MtrunA17_Chr5g0399781 | 50.000 | 54 | 26 | 1 | 3 | 56 | 12 | 64 | 7.51e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 94 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAAAAGCTTCAAAGTCTA+TGG | 0.184389 | 1_3:+23400376 | Msa0110540:CDS |
| CCACAAGCATTACATAGTAT+TGG | 0.402584 | 1_3:-23399765 | None:intergenic |
| TGGAGATTGGTTTGAAAGCT+CGG | 0.447768 | 1_3:-23399892 | None:intergenic |
| GAAACTAAACTTTGGCGTAA+AGG | 0.456777 | 1_3:+23399729 | Msa0110540:CDS |
| TGCTTGTGGACTACATTTCA+AGG | 0.461822 | 1_3:+23399779 | Msa0110540:CDS |
| ATCAGACCTTGACGTTGAAA+AGG | 0.465983 | 1_3:+23399854 | Msa0110540:CDS |
| GGACTACATTTCAAGGCTAA+AGG | 0.474721 | 1_3:+23399786 | Msa0110540:CDS |
| TCTTCGGAAGATAGTTATCA+AGG | 0.478433 | 1_3:-23399812 | None:intergenic |
| TGTATACCAAGCAATGAGAT+AGG | 0.478624 | 1_3:+23400465 | Msa0110540:CDS |
| ATCGATTGTGAAGAATCAAA+TGG | 0.491491 | 1_3:+23400344 | Msa0110540:CDS |
| GGAGATTGGTTTGAAAGCTC+GGG | 0.493147 | 1_3:-23399891 | None:intergenic |
| CCAAGCAATGAGATAGGCAT+TGG | 0.496933 | 1_3:+23400471 | Msa0110540:CDS |
| ATTTGATTCTTCACAATCGA+TGG | 0.523804 | 1_3:-23400342 | None:intergenic |
| CATAGTATTGGATTCTCATA+CGG | 0.534177 | 1_3:-23399753 | None:intergenic |
| ACAAAAGCTTCAAAGTCTAT+GGG | 0.556384 | 1_3:+23400377 | Msa0110540:CDS |
| CCAATGCCTATCTCATTGCT+TGG | 0.566560 | 1_3:-23400471 | None:intergenic |
| GAGCTTTCAAACCAATCTCC+AGG | 0.568278 | 1_3:+23399894 | Msa0110540:CDS |
| CCAATACTATGTAATGCTTG+TGG | 0.568792 | 1_3:+23399765 | Msa0110540:CDS |
| CTTCGGAAGATAGTTATCAA+GGG | 0.629882 | 1_3:-23399811 | None:intergenic |
| GATTGTGAAGAATCAAATGG+AGG | 0.680011 | 1_3:+23400347 | Msa0110540:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTATATTTGTTGAAAAAT+GGG | - | chr1_3:23399955-23399974 | None:intergenic | 10.0% |
| !!! | TTTTAATTTTTTTTGTAGAA+AGG | + | chr1_3:23400316-23400335 | Msa0110540:intron | 10.0% |
| !!! | ATTTATATTTGTTGAAAAAT+GGG | - | chr1_3:23399955-23399974 | None:intergenic | 10.0% |
| !!! | TTTTAATTTTTTTTGTAGAA+AGG | + | chr1_3:23400316-23400335 | Msa0110540:intron | 10.0% |
| !!! | TTTATATTTGTTGAAAAATG+GGG | - | chr1_3:23399954-23399973 | None:intergenic | 15.0% |
| !!! | GATTTATATTTGTTGAAAAA+TGG | - | chr1_3:23399956-23399975 | None:intergenic | 15.0% |
| !!! | TTTATATTTGTTGAAAAATG+GGG | - | chr1_3:23399954-23399973 | None:intergenic | 15.0% |
| !!! | GATTTATATTTGTTGAAAAA+TGG | - | chr1_3:23399956-23399975 | None:intergenic | 15.0% |
| !!! | TTATTGCATTTTTTATTACC+TGG | - | chr1_3:23399915-23399934 | None:intergenic | 20.0% |
| !!! | ATTGTAAGAATTTTCTTAAG+TGG | + | chr1_3:23400009-23400028 | Msa0110540:intron | 20.0% |
| !! | AACACTACTATTTATATATG+AGG | + | chr1_3:23400060-23400079 | Msa0110540:intron | 20.0% |
| !!! | TTTTGAATGAATTGTATTGA+TGG | + | chr1_3:23400153-23400172 | Msa0110540:intron | 20.0% |
| !!! | TTTGAATGAATTGTATTGAT+GGG | + | chr1_3:23400154-23400173 | Msa0110540:intron | 20.0% |
| !! | TTATATGTATACATCATACA+CGG | - | chr1_3:23400294-23400313 | None:intergenic | 20.0% |
| !!! | TTATTGCATTTTTTATTACC+TGG | - | chr1_3:23399915-23399934 | None:intergenic | 20.0% |
| !!! | ATTGTAAGAATTTTCTTAAG+TGG | + | chr1_3:23400009-23400028 | Msa0110540:intron | 20.0% |
| !! | AACACTACTATTTATATATG+AGG | + | chr1_3:23400060-23400079 | Msa0110540:intron | 20.0% |
| !!! | TTTTGAATGAATTGTATTGA+TGG | + | chr1_3:23400153-23400172 | Msa0110540:intron | 20.0% |
| !!! | TTTGAATGAATTGTATTGAT+GGG | + | chr1_3:23400154-23400173 | Msa0110540:intron | 20.0% |
| !! | TTATATGTATACATCATACA+CGG | - | chr1_3:23400294-23400313 | None:intergenic | 20.0% |
| !! | TTTTGACAGAAACTAAACTT+TGG | + | chr1_3:23399721-23399740 | Msa0110540:CDS | 25.0% |
| !!! | ATTTTTTATTACCTGGAGAT+TGG | - | chr1_3:23399908-23399927 | None:intergenic | 25.0% |
| ! | TTTCTTAAGTGGTACAATTT+AGG | + | chr1_3:23400020-23400039 | Msa0110540:intron | 25.0% |
| ! | ATTGACAATGAAAGGAATTA+GGG | - | chr1_3:23400100-23400119 | None:intergenic | 25.0% |
| ! | TATTGACAATGAAAGGAATT+AGG | - | chr1_3:23400101-23400120 | None:intergenic | 25.0% |
| !! | TTGAATGAATTGTATTGATG+GGG | + | chr1_3:23400155-23400174 | Msa0110540:intron | 25.0% |
| ! | TATCCATAAATGTATGAATG+TGG | - | chr1_3:23400203-23400222 | None:intergenic | 25.0% |
| !! | TTTTGACAGAAACTAAACTT+TGG | + | chr1_3:23399721-23399740 | Msa0110540:CDS | 25.0% |
| !!! | ATTTTTTATTACCTGGAGAT+TGG | - | chr1_3:23399908-23399927 | None:intergenic | 25.0% |
| ! | TTTCTTAAGTGGTACAATTT+AGG | + | chr1_3:23400020-23400039 | Msa0110540:intron | 25.0% |
| ! | ATTGACAATGAAAGGAATTA+GGG | - | chr1_3:23400100-23400119 | None:intergenic | 25.0% |
| ! | TATTGACAATGAAAGGAATT+AGG | - | chr1_3:23400101-23400120 | None:intergenic | 25.0% |
| !! | TTGAATGAATTGTATTGATG+GGG | + | chr1_3:23400155-23400174 | Msa0110540:intron | 25.0% |
| ! | TATCCATAAATGTATGAATG+TGG | - | chr1_3:23400203-23400222 | None:intergenic | 25.0% |
| ! | CATAGTATTGGATTCTCATA+CGG | - | chr1_3:23399756-23399775 | None:intergenic | 30.0% |
| !! | GTCTGATAACTGATTTTGTT+GGG | - | chr1_3:23399841-23399860 | None:intergenic | 30.0% |
| ATGCTCTTATTGACAATGAA+AGG | - | chr1_3:23400108-23400127 | None:intergenic | 30.0% | |
| ACTCCACATTCATACATTTA+TGG | + | chr1_3:23400197-23400216 | Msa0110540:intron | 30.0% | |
| ATTTGATTCTTCACAATCGA+TGG | - | chr1_3:23400345-23400364 | None:intergenic | 30.0% | |
| ATCGATTGTGAAGAATCAAA+TGG | + | chr1_3:23400344-23400363 | Msa0110540:CDS | 30.0% | |
| AACAAAAGCTTCAAAGTCTA+TGG | + | chr1_3:23400376-23400395 | Msa0110540:CDS | 30.0% | |
| ACAAAAGCTTCAAAGTCTAT+GGG | + | chr1_3:23400377-23400396 | Msa0110540:CDS | 30.0% | |
| ! | CATAGTATTGGATTCTCATA+CGG | - | chr1_3:23399756-23399775 | None:intergenic | 30.0% |
| !! | GTCTGATAACTGATTTTGTT+GGG | - | chr1_3:23399841-23399860 | None:intergenic | 30.0% |
| ATGCTCTTATTGACAATGAA+AGG | - | chr1_3:23400108-23400127 | None:intergenic | 30.0% | |
| ACTCCACATTCATACATTTA+TGG | + | chr1_3:23400197-23400216 | Msa0110540:intron | 30.0% | |
| ATTTGATTCTTCACAATCGA+TGG | - | chr1_3:23400345-23400364 | None:intergenic | 30.0% | |
| ATCGATTGTGAAGAATCAAA+TGG | + | chr1_3:23400344-23400363 | Msa0110540:CDS | 30.0% | |
| AACAAAAGCTTCAAAGTCTA+TGG | + | chr1_3:23400376-23400395 | Msa0110540:CDS | 30.0% | |
| ACAAAAGCTTCAAAGTCTAT+GGG | + | chr1_3:23400377-23400396 | Msa0110540:CDS | 30.0% | |
| GAAACTAAACTTTGGCGTAA+AGG | + | chr1_3:23399729-23399748 | Msa0110540:CDS | 35.0% | |
| CCACAAGCATTACATAGTAT+TGG | - | chr1_3:23399768-23399787 | None:intergenic | 35.0% | |
| CCAATACTATGTAATGCTTG+TGG | + | chr1_3:23399765-23399784 | Msa0110540:CDS | 35.0% | |
| CTTCGGAAGATAGTTATCAA+GGG | - | chr1_3:23399814-23399833 | None:intergenic | 35.0% | |
| TCTTCGGAAGATAGTTATCA+AGG | - | chr1_3:23399815-23399834 | None:intergenic | 35.0% | |
| ! | TGATTTTGTTGGGATGTCTT+CGG | - | chr1_3:23399831-23399850 | None:intergenic | 35.0% |
| !! | GGTCTGATAACTGATTTTGT+TGG | - | chr1_3:23399842-23399861 | None:intergenic | 35.0% |
| AGCATTCATTTGAGTAGACA+CGG | + | chr1_3:23400123-23400142 | Msa0110540:intron | 35.0% | |
| ATTTGAGTAGACACGGTATT+AGG | + | chr1_3:23400130-23400149 | Msa0110540:intron | 35.0% | |
| ! | GAATTGTATTGATGGGGATT+AGG | + | chr1_3:23400161-23400180 | Msa0110540:intron | 35.0% |
| TCATACACGGTACATATCAA+TGG | - | chr1_3:23400281-23400300 | None:intergenic | 35.0% | |
| GATTGTGAAGAATCAAATGG+AGG | + | chr1_3:23400347-23400366 | Msa0110540:CDS | 35.0% | |
| TGTATACCAAGCAATGAGAT+AGG | + | chr1_3:23400465-23400484 | Msa0110540:CDS | 35.0% | |
| GAAACTAAACTTTGGCGTAA+AGG | + | chr1_3:23399729-23399748 | Msa0110540:CDS | 35.0% | |
| CCACAAGCATTACATAGTAT+TGG | - | chr1_3:23399768-23399787 | None:intergenic | 35.0% | |
| CCAATACTATGTAATGCTTG+TGG | + | chr1_3:23399765-23399784 | Msa0110540:CDS | 35.0% | |
| CTTCGGAAGATAGTTATCAA+GGG | - | chr1_3:23399814-23399833 | None:intergenic | 35.0% | |
| TCTTCGGAAGATAGTTATCA+AGG | - | chr1_3:23399815-23399834 | None:intergenic | 35.0% | |
| ! | TGATTTTGTTGGGATGTCTT+CGG | - | chr1_3:23399831-23399850 | None:intergenic | 35.0% |
| !! | GGTCTGATAACTGATTTTGT+TGG | - | chr1_3:23399842-23399861 | None:intergenic | 35.0% |
| AGCATTCATTTGAGTAGACA+CGG | + | chr1_3:23400123-23400142 | Msa0110540:intron | 35.0% | |
| ATTTGAGTAGACACGGTATT+AGG | + | chr1_3:23400130-23400149 | Msa0110540:intron | 35.0% | |
| ! | GAATTGTATTGATGGGGATT+AGG | + | chr1_3:23400161-23400180 | Msa0110540:intron | 35.0% |
| TCATACACGGTACATATCAA+TGG | - | chr1_3:23400281-23400300 | None:intergenic | 35.0% | |
| GATTGTGAAGAATCAAATGG+AGG | + | chr1_3:23400347-23400366 | Msa0110540:CDS | 35.0% | |
| TGTATACCAAGCAATGAGAT+AGG | + | chr1_3:23400465-23400484 | Msa0110540:CDS | 35.0% | |
| ! | TGCTTGTGGACTACATTTCA+AGG | + | chr1_3:23399779-23399798 | Msa0110540:CDS | 40.0% |
| GGACTACATTTCAAGGCTAA+AGG | + | chr1_3:23399786-23399805 | Msa0110540:CDS | 40.0% | |
| ATCAGACCTTGACGTTGAAA+AGG | + | chr1_3:23399854-23399873 | Msa0110540:CDS | 40.0% | |
| ! | CAGATTCCTTTTCAACGTCA+AGG | - | chr1_3:23399863-23399882 | None:intergenic | 40.0% |
| !! | TGGAGATTGGTTTGAAAGCT+CGG | - | chr1_3:23399895-23399914 | None:intergenic | 40.0% |
| ! | TGCTTGTGGACTACATTTCA+AGG | + | chr1_3:23399779-23399798 | Msa0110540:CDS | 40.0% |
| GGACTACATTTCAAGGCTAA+AGG | + | chr1_3:23399786-23399805 | Msa0110540:CDS | 40.0% | |
| ATCAGACCTTGACGTTGAAA+AGG | + | chr1_3:23399854-23399873 | Msa0110540:CDS | 40.0% | |
| ! | CAGATTCCTTTTCAACGTCA+AGG | - | chr1_3:23399863-23399882 | None:intergenic | 40.0% |
| !! | TGGAGATTGGTTTGAAAGCT+CGG | - | chr1_3:23399895-23399914 | None:intergenic | 40.0% |
| !! | GGAGATTGGTTTGAAAGCTC+GGG | - | chr1_3:23399894-23399913 | None:intergenic | 45.0% |
| GAGCTTTCAAACCAATCTCC+AGG | + | chr1_3:23399894-23399913 | Msa0110540:CDS | 45.0% | |
| CCAATGCCTATCTCATTGCT+TGG | - | chr1_3:23400474-23400493 | None:intergenic | 45.0% | |
| CCAAGCAATGAGATAGGCAT+TGG | + | chr1_3:23400471-23400490 | Msa0110540:CDS | 45.0% | |
| !! | GGAGATTGGTTTGAAAGCTC+GGG | - | chr1_3:23399894-23399913 | None:intergenic | 45.0% |
| GAGCTTTCAAACCAATCTCC+AGG | + | chr1_3:23399894-23399913 | Msa0110540:CDS | 45.0% | |
| CCAATGCCTATCTCATTGCT+TGG | - | chr1_3:23400474-23400493 | None:intergenic | 45.0% | |
| CCAAGCAATGAGATAGGCAT+TGG | + | chr1_3:23400471-23400490 | Msa0110540:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_3 | gene | 23399717 | 23400539 | 23399717 | ID=Msa0110540;Name=Msa0110540 |
| chr1_3 | mRNA | 23399717 | 23400539 | 23399717 | ID=Msa0110540-mRNA-1;Parent=Msa0110540;Name=Msa0110540-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0|0|0|1|1|1|2|0|134 |
| chr1_3 | exon | 23399717 | 23399915 | 23399717 | ID=Msa0110540-mRNA-1:exon:8218;Parent=Msa0110540-mRNA-1 |
| chr1_3 | exon | 23400334 | 23400539 | 23400334 | ID=Msa0110540-mRNA-1:exon:8219;Parent=Msa0110540-mRNA-1 |
| chr1_3 | CDS | 23399717 | 23399915 | 23399717 | ID=Msa0110540-mRNA-1:cds;Parent=Msa0110540-mRNA-1 |
| chr1_3 | CDS | 23400334 | 23400539 | 23400334 | ID=Msa0110540-mRNA-1:cds;Parent=Msa0110540-mRNA-1 |
| Gene Sequence |
| Protein sequence |
