AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0111120

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0111120 MtrunA17_Chr1g0169431 98.770 244 3 0 1 244 1 244 0.0 498
Msa0111120 MtrunA17_Chr5g0395391 71.489 235 52 3 1 235 12 231 9.78e-105 306
Msa0111120 MtrunA17_Chr5g0395391 76.147 109 26 0 136 244 234 342 1.01e-56 184
Msa0111120 MtrunA17_Chr8g0340401 47.340 188 84 7 63 241 114 295 3.01e-39 139
Msa0111120 MtrunA17_Chr8g0372291 72.000 75 21 0 170 244 216 290 4.03e-36 129
Msa0111120 MtrunA17_Chr2g0326771 46.584 161 54 4 99 236 129 280 1.67e-35 128
Msa0111120 MtrunA17_Chr1g0146231 43.810 210 71 7 63 241 110 303 4.66e-35 127
Msa0111120 MtrunA17_Chr3g0140331 44.554 202 69 8 63 241 141 322 6.76e-35 127
Msa0111120 MtrunA17_Chr3g0145121 36.260 262 122 9 6 241 42 284 8.94e-35 126
Msa0111120 MtrunA17_Chr4g0035891 50.000 140 44 5 124 239 141 278 2.17e-33 122
Msa0111120 MtrunA17_Chr5g0404981 71.233 73 21 0 172 244 300 372 2.53e-33 124
Msa0111120 MtrunA17_Chr6g0461591 76.471 68 16 0 171 238 191 258 5.42e-33 120
Msa0111120 MtrunA17_Chr7g0253101 74.648 71 18 0 166 236 182 252 6.59e-33 120
Msa0111120 MtrunA17_Chr1g0199671 70.130 77 21 1 160 236 232 306 2.18e-32 120
Msa0111120 MtrunA17_Chr4g0028961 74.627 67 17 0 172 238 232 298 3.01e-32 120
Msa0111120 MtrunA17_Chr1g0213161 71.642 67 19 0 170 236 150 216 1.36e-31 116
Msa0111120 MtrunA17_Chr7g0232671 55.224 67 28 1 172 238 187 251 3.82e-19 83.6
Msa0111120 MtrunA17_Chr7g0232701 47.126 87 44 2 149 233 48 134 4.18e-18 78.2
Msa0111120 MtrunA17_Chr7g0232681 46.739 92 45 3 149 238 107 196 1.90e-17 77.8
Msa0111120 MtrunA17_Chr7g0271861 54.762 42 19 0 166 207 16 57 9.49e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0111120 AT3G24050.1 80.000 75 15 0 170 244 192 266 2.47e-40 140
Msa0111120 AT4G32890.1 39.636 275 98 11 7 241 20 266 6.46e-38 135
Msa0111120 AT4G36240.1 38.340 253 104 6 6 239 12 231 6.98e-36 127
Msa0111120 AT4G34680.1 46.479 142 63 3 111 242 112 250 4.91e-35 126
Msa0111120 AT4G34680.2 46.479 142 63 3 111 242 112 250 4.91e-35 126
Msa0111120 AT5G66320.2 79.412 68 14 0 172 239 249 316 8.80e-35 127
Msa0111120 AT5G66320.1 79.412 68 14 0 172 239 249 316 8.80e-35 127
Msa0111120 AT5G25830.1 77.143 70 16 0 172 241 219 288 1.23e-33 124
Msa0111120 AT3G51080.1 72.603 73 20 0 172 244 221 293 2.74e-33 122
Msa0111120 AT3G60530.1 75.362 69 17 0 171 239 157 225 2.88e-33 120
Msa0111120 AT1G08010.2 72.464 69 19 0 170 238 218 286 2.82e-32 120
Msa0111120 AT1G08010.1 72.464 69 19 0 170 238 218 286 2.82e-32 120
Msa0111120 AT1G08010.3 72.464 69 19 0 170 238 218 286 2.82e-32 120
Msa0111120 AT2G45050.1 75.000 68 17 0 172 239 179 246 3.84e-32 118
Msa0111120 AT1G08000.2 64.634 82 29 0 157 238 203 284 7.00e-32 119
Msa0111120 AT1G08000.1 64.634 82 29 0 157 238 203 284 7.00e-32 119
Msa0111120 AT3G54810.1 75.000 68 17 0 172 239 229 296 6.57e-31 117
Msa0111120 AT3G54810.2 75.000 68 17 0 172 239 229 296 6.57e-31 117
Msa0111120 AT3G45170.1 60.294 68 27 0 172 239 115 182 2.38e-27 104
Msa0111120 AT2G28340.1 66.667 63 21 0 174 236 193 255 9.81e-26 102

Find 102 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGACAAGCAAAATGATTTA+AGG 0.141755 1_3:+24626120 None:intergenic
TCAGTCTCTCAATTCTCTTT+TGG 0.180061 1_3:-24628834 Msa0111120:five_prime_UTR
CATGCCAAAAGTTAGGATTT+TGG 0.206218 1_3:-24626255 Msa0111120:three_prime_UTR
TGCTTATCTGAACTAATTCT+AGG 0.234887 1_3:-24625981 Msa0111120:three_prime_UTR
CTTGTGCTGTTCTCGAGCTT+TGG 0.240759 1_3:+24626754 None:intergenic
ATGTACTTTGATTGAGGATT+AGG 0.241750 1_3:-24626044 Msa0111120:three_prime_UTR
TTGTGCTGTTCTCGAGCTTT+GGG 0.269770 1_3:+24626755 None:intergenic
AGTGAAGGAAAGGCTTTCTT+AGG 0.280723 1_3:+24627447 None:intergenic
GTCCAACAAAGATGCATTTC+CGG 0.285678 1_3:-24626842 Msa0111120:CDS
GACATTTCCTCCCAATGGTT+GGG 0.293078 1_3:+24626542 None:intergenic
CTATTTGCAGGACGGTATTC+AGG 0.302785 1_3:+24626415 None:intergenic
CGAAAAGTTGGGCTATTTGC+AGG 0.311001 1_3:+24626403 None:intergenic
CATGCATTACAAAGTGTCTT+TGG 0.315428 1_3:+24626469 None:intergenic
CATTTAGCATGCCAAAAGTT+AGG 0.327033 1_3:-24626262 Msa0111120:three_prime_UTR
AAAACCAAAATCCTAACTTT+TGG 0.331501 1_3:+24626251 None:intergenic
CAAAGTGTCTTTGGACCATT+TGG 0.337970 1_3:+24626478 None:intergenic
TGTACTTTGATTGAGGATTA+GGG 0.355942 1_3:-24626043 Msa0111120:three_prime_UTR
GAAGGTGGATCACTGCATTC+TGG 0.356189 1_3:+24627420 None:intergenic
GTTGTTTCCACGGGAATTGT+AGG 0.366200 1_3:+24626602 None:intergenic
CTCTCTTGCCTGCTACTAGC+TGG 0.381715 1_3:-24628779 Msa0111120:five_prime_UTR
TGGTTCTGTGGACGACCTCT+TGG 0.388206 1_3:-24627511 Msa0111120:CDS
GATGAGGAAGCAGAAGGGAA+TGG 0.399465 1_3:-24626341 Msa0111120:CDS
TTTGTAATGCATGTGGGGTT+AGG 0.415099 1_3:-24626459 Msa0111120:CDS
ACTTACAGAAAGTGAATTGA+TGG 0.418526 1_3:+24627371 None:intergenic
CCACGGGAATTGTAGGTTTG+AGG 0.425374 1_3:+24626609 None:intergenic
AAGCAGAAGGGAATGGGAAC+GGG 0.428545 1_3:-24626334 Msa0111120:CDS
TGTGATTGTAGCCACTTAAG+AGG 0.429240 1_3:+24626695 None:intergenic
AGCCGGAAATGCATCTTTGT+TGG 0.438765 1_3:+24626840 None:intergenic
TGCATGTCACTGCGAAAAGT+TGG 0.440834 1_3:+24626391 None:intergenic
TGAGTTGTCCTGCATTCAAC+CGG 0.442354 1_3:-24626803 Msa0111120:CDS
ACATTTCCTCCCAATGGTTG+GGG 0.449081 1_3:+24626543 None:intergenic
ATTTGATGCTTCAAAAGATC+CGG 0.450692 1_3:+24626784 None:intergenic
GACTTGCTCCGTGCACGGAC+AGG 0.458205 1_3:+24626664 None:intergenic
TTCGCTCAATCCATTGGCCC+TGG 0.469600 1_3:-24627400 Msa0111120:CDS
GACAAGAGCCAGCTAGTAGC+AGG 0.476760 1_3:+24628771 None:intergenic
CCAGGAGGAGGTTGCAGAAG+AGG 0.477241 1_3:-24626878 Msa0111120:intron
GTGGAGATGAGGAAGCAGAA+GGG 0.478182 1_3:-24626346 Msa0111120:CDS
TTGCAGAAGAGGAGCTTGAA+TGG 0.482772 1_3:-24626867 Msa0111120:CDS
GTCATTGATAAAATGAAAAG+TGG 0.484461 1_3:-24626305 Msa0111120:three_prime_UTR
ATGAGGAAGCAGAAGGGAAT+GGG 0.486460 1_3:-24626340 Msa0111120:CDS
GTGAAACAAACCCCAACCAT+TGG 0.493226 1_3:-24626553 Msa0111120:CDS
TTGGTACTCGGTTGTTTCCA+CGG 0.494137 1_3:+24626592 None:intergenic
AACAGCATTACCCTCTTAAG+TGG 0.500575 1_3:-24626706 Msa0111120:CDS
GCATGTCACTGCGAAAAGTT+GGG 0.502991 1_3:+24626392 None:intergenic
TGGTACTCGGTTGTTTCCAC+GGG 0.505676 1_3:+24626593 None:intergenic
CAATGGCGTGCAGGTCCAAA+TGG 0.508669 1_3:-24626493 Msa0111120:CDS
TTGGATTTCTCATCTGACAT+AGG 0.508816 1_3:-24627492 Msa0111120:CDS
AGTGGAGATGAGGAAGCAGA+AGG 0.514467 1_3:-24626347 Msa0111120:CDS
TTCTGGTTTGAGTGAAGGAA+AGG 0.515866 1_3:+24627437 None:intergenic
TGACATTTCCTCCCAATGGT+TGG 0.516568 1_3:+24626541 None:intergenic
CAAAAGATCCGGTTGAATGC+AGG 0.520121 1_3:+24626795 None:intergenic
TGTGGGGTTAGGTATAAGTC+TGG 0.520333 1_3:-24626448 Msa0111120:CDS
GGTAACATGTACTTTGATTG+AGG 0.520464 1_3:-24626050 Msa0111120:three_prime_UTR
TCCACCTTCGCTCAATCCAT+TGG 0.521499 1_3:-24627406 Msa0111120:CDS
GATGGAGGCCCTTGGTTCTG+TGG 0.522422 1_3:-24627523 Msa0111120:CDS
TGAAACAAACCCCAACCATT+GGG 0.524385 1_3:-24626552 Msa0111120:CDS
GTTCAGATAAGCATAATGAA+TGG 0.527844 1_3:+24625991 None:intergenic
AATTCTAGGTTGATGAATAG+TGG 0.527863 1_3:-24625967 Msa0111120:three_prime_UTR
GTGAATTGATGGGATCATCC+AGG 0.528433 1_3:+24627382 None:intergenic
ATGGGATCATCCAGGGCCAA+TGG 0.530010 1_3:+24627390 None:intergenic
CTGCATTCTGGTTTGAGTGA+AGG 0.532294 1_3:+24627432 None:intergenic
TCATCTCCACTACTTTCCTG+TGG 0.533667 1_3:+24626359 None:intergenic
TCTCGTAGCAAGCCTCGCCT+TGG 0.534627 1_3:-24626643 Msa0111120:CDS
AGTACACAAACGTGAAAAGC+TGG 0.538176 1_3:-24628740 Msa0111120:intron
GATACTCCACAATGGCGTGC+AGG 0.539263 1_3:-24626502 Msa0111120:CDS
TCAACAAACGTTTCAACAGC+CGG 0.539345 1_3:+24626823 None:intergenic
TTGAGGCATCAGCAAGGCCA+AGG 0.541565 1_3:+24626626 None:intergenic
GAAGCAGAAGGGAATGGGAA+CGG 0.544276 1_3:-24626335 Msa0111120:CDS
GCCAATGGATTGAGCGAAGG+TGG 0.546210 1_3:+24627405 None:intergenic
ACTTAAGAGGGTAATGCTGT+TGG 0.550014 1_3:+24626708 None:intergenic
CATCTCCACTACTTTCCTGT+GGG 0.557649 1_3:+24626360 None:intergenic
CTGAAAGAACCAACGAGAGA+TGG 0.561073 1_3:+24628853 None:intergenic
AGAACCAACGAGAGATGGTA+AGG 0.566590 1_3:+24628858 None:intergenic
TGAAGTTCCCTGTCCGTGCA+CGG 0.571256 1_3:-24626672 Msa0111120:CDS
TTTGGACCTGCACGCCATTG+TGG 0.572595 1_3:+24626496 None:intergenic
ACTTGCTCCGTGCACGGACA+GGG 0.574860 1_3:+24626665 None:intergenic
TACGAGACTTGCTCCGTGCA+CGG 0.578328 1_3:+24626659 None:intergenic
CGTGCCTTACCATCTCTCGT+TGG 0.580045 1_3:-24628862 None:intergenic
ATGATGACATTTCCTCCCAA+TGG 0.580868 1_3:+24626537 None:intergenic
ACAGGAAAGTAGTGGAGATG+AGG 0.582547 1_3:-24626357 Msa0111120:CDS
TGTGCTGTTCTCGAGCTTTG+GGG 0.589584 1_3:+24626756 None:intergenic
TGAATTGATGGGATCATCCA+GGG 0.600445 1_3:+24627383 None:intergenic
GCATCAGCAAGGCCAAGGCG+AGG 0.601336 1_3:+24626631 None:intergenic
GACACTTTGTAATGCATGTG+GGG 0.601729 1_3:-24626464 Msa0111120:CDS
AAGACACTTTGTAATGCATG+TGG 0.602236 1_3:-24626466 Msa0111120:CDS
TAATTCCCACAGGAAAGTAG+TGG 0.604924 1_3:-24626365 Msa0111120:CDS
GAGGTCGTCCACAGAACCAA+GGG 0.608143 1_3:+24627515 None:intergenic
AGACACTTTGTAATGCATGT+GGG 0.608576 1_3:-24626465 Msa0111120:CDS
CTTACAGAAAGTGAATTGAT+GGG 0.611737 1_3:+24627372 None:intergenic
GTGATTGTAGCCACTTAAGA+GGG 0.622729 1_3:+24626696 None:intergenic
ACATGCATTCTAATTCCCAC+AGG 0.630282 1_3:-24626375 Msa0111120:CDS
AACAAACCCCAACCATTGGG+AGG 0.635541 1_3:-24626549 Msa0111120:CDS
AGGGCCAATGGATTGAGCGA+AGG 0.639769 1_3:+24627402 None:intergenic
AGAGGTCGTCCACAGAACCA+AGG 0.642044 1_3:+24627514 None:intergenic
GGGAGGAAATGTCATCATTG+TGG 0.646984 1_3:-24626532 Msa0111120:CDS
CCTCAAACCTACAATTCCCG+TGG 0.649034 1_3:-24626609 Msa0111120:CDS
GACGGTATTCAGGTACAAGT+CGG 0.649278 1_3:+24626425 None:intergenic
AAGTTGGGCTATTTGCAGGA+CGG 0.658089 1_3:+24626407 None:intergenic
TTTCTCATCTGACATAGGTG+AGG 0.665571 1_3:-24627487 Msa0111120:CDS
GTAGGTTTGAGGCATCAGCA+AGG 0.716777 1_3:+24626620 None:intergenic
TGTCAGATGAGAAATCCAAG+AGG 0.747403 1_3:+24627496 None:intergenic
GAGTTGATGATACTCCACAA+TGG 0.801331 1_3:-24626510 Msa0111120:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTAATATTTGTTTGTA+CGG - chr1_3:24627209-24627228 Msa0111120:intron 10.0%
!!! TTTTATTATTATTTGTGAAG+TGG - chr1_3:24626232-24626251 Msa0111120:three_prime_UTR 15.0%
!! TTTATTATTATTTGTGAAGT+GGG - chr1_3:24626233-24626252 Msa0111120:three_prime_UTR 15.0%
!!! TTTGTTTAAGGTTTAATTTA+AGG - chr1_3:24626336-24626355 Msa0111120:CDS 15.0%
!!! TTTTCTAATCATTTTCATAT+TGG - chr1_3:24626421-24626440 Msa0111120:CDS 15.0%
!! AAAAAACATAGCAAAGATTA+AGG + chr1_3:24626112-24626131 None:intergenic 20.0%
!! ATTTGAATATGCTGAAAATT+TGG - chr1_3:24626208-24626227 Msa0111120:three_prime_UTR 20.0%
!! TATTGAAGGAATAATGAAAT+GGG - chr1_3:24626264-24626283 Msa0111120:three_prime_UTR 20.0%
!! AACAAAGAAAAACAAGAAAA+TGG + chr1_3:24626322-24626341 None:intergenic 20.0%
!!! TTCTTGTTTTTCTTTGTTTA+AGG - chr1_3:24626324-24626343 Msa0111120:exon 20.0%
!!! TGCTTATTTTTATGTTATGT+TGG - chr1_3:24626367-24626386 Msa0111120:CDS 20.0%
!! AAAGATTAAAGAACTTCATT+TGG + chr1_3:24626568-24626587 None:intergenic 20.0%
!!! GTTGTTTTTATAAGCTATAT+TGG - chr1_3:24626725-24626744 Msa0111120:CDS 20.0%
!! AAAAGTAACTCAAGTAATAA+AGG + chr1_3:24626937-24626956 None:intergenic 20.0%
!!! CTTTTTATTGTGTTTATGAA+TGG - chr1_3:24626979-24626998 Msa0111120:intron 20.0%
!! TTAAAAAATAGTGAAAAGCA+TGG + chr1_3:24627197-24627216 None:intergenic 20.0%
!! ACAGATAAAAATAACAGTAT+AGG + chr1_3:24627240-24627259 None:intergenic 20.0%
!!! AAGTATTTTGAAAAGGTTAA+TGG + chr1_3:24627461-24627480 None:intergenic 20.0%
!!! TAGATTAAAATTTTGCAGTT+AGG - chr1_3:24628651-24628670 Msa0111120:intron 20.0%
!!! AGATTAAAATTTTGCAGTTA+GGG - chr1_3:24628652-24628671 Msa0111120:intron 20.0%
! AAAAACAAGCAAGAAAAAGA+GGG + chr1_3:24626155-24626174 None:intergenic 25.0%
! TAAAAACAAGCAAGAAAAAG+AGG + chr1_3:24626156-24626175 None:intergenic 25.0%
! GTATTGAAGGAATAATGAAA+TGG - chr1_3:24626263-24626282 Msa0111120:three_prime_UTR 25.0%
! AAAGAACAGAAACAATGAAT+TGG + chr1_3:24626537-24626556 None:intergenic 25.0%
! TGTAAAATCGTATACTTTAC+TGG - chr1_3:24626871-24626890 Msa0111120:CDS 25.0%
!!! ATACTTTACTGGTTTTTGTA+TGG - chr1_3:24626882-24626901 Msa0111120:intron 25.0%
! TAATGTAAAGAACTTATTCC+AGG + chr1_3:24627017-24627036 None:intergenic 25.0%
! TTTCATGTATTGTAATCACA+AGG - chr1_3:24627094-24627113 Msa0111120:intron 25.0%
! TGAAAATTTCATAGTAGGTT+TGG + chr1_3:24627126-24627145 None:intergenic 25.0%
!! AAACCTACTATGAAATTTTC+AGG - chr1_3:24627125-24627144 Msa0111120:intron 25.0%
! AATCCTGAAAATTTCATAGT+AGG + chr1_3:24627131-24627150 None:intergenic 25.0%
!!! TGTATATTTGTTTTCAGAGA+TGG - chr1_3:24627257-24627276 Msa0111120:intron 25.0%
!!! ATCAAGCAAGTATTTTGAAA+AGG + chr1_3:24627468-24627487 None:intergenic 25.0%
! AAGGAAAACAAGTAAAAATC+TGG + chr1_3:24627626-24627645 None:intergenic 25.0%
! AACTCATAAGTATGGAAAAA+AGG + chr1_3:24627876-24627895 None:intergenic 25.0%
! GTCATTGATAAAATGAAAAG+TGG - chr1_3:24628493-24628512 Msa0111120:intron 25.0%
!! AAAACCAAAATCCTAACTTT+TGG + chr1_3:24628550-24628569 None:intergenic 25.0%
! TTGACAAGCAAAATGATTTA+AGG + chr1_3:24628681-24628700 None:intergenic 25.0%
CAGAGGAAAATTAAACATAG+TGG + chr1_3:24626184-24626203 None:intergenic 30.0%
AGCATATTCAAATAACACAG+AGG + chr1_3:24626201-24626220 None:intergenic 30.0%
! TACGATTTTACACATCGTAT+TGG + chr1_3:24626863-24626882 None:intergenic 30.0%
!!! GAATGGTGTTTTATTTTACC+TGG - chr1_3:24626996-24627015 Msa0111120:intron 30.0%
!! ATATTTGTTTTCAGAGATGG+AGG - chr1_3:24627260-24627279 Msa0111120:intron 30.0%
CTTACAGAAAGTGAATTGAT+GGG + chr1_3:24627429-24627448 None:intergenic 30.0%
ACTTACAGAAAGTGAATTGA+TGG + chr1_3:24627430-24627449 None:intergenic 30.0%
TTCACTTTCTGTAAGTTTGA+CGG - chr1_3:24627434-24627453 Msa0111120:CDS 30.0%
!!! TATCTAGTTTTGACTTGTTG+AGG - chr1_3:24627492-24627511 Msa0111120:CDS 30.0%
CACTAGCTAATATTCTACTT+TGG + chr1_3:24627600-24627619 None:intergenic 30.0%
TCCTTTAAGCATCAATGTAT+TGG - chr1_3:24627641-24627660 Msa0111120:intron 30.0%
CATGAAAACTTTGATGCAAA+CGG + chr1_3:24627717-24627736 None:intergenic 30.0%
GCACATTAAACTCATAAGTA+TGG + chr1_3:24627884-24627903 None:intergenic 30.0%
ATTTGATGCTTCAAAAGATC+CGG + chr1_3:24628017-24628036 None:intergenic 30.0%
!!! CACTTTTTCTTTGGAAGTTT+TGG + chr1_3:24628228-24628247 None:intergenic 30.0%
!!! GGTTTGTTTCACTTTTTCTT+TGG + chr1_3:24628237-24628256 None:intergenic 30.0%
!! ATTTTAGTCCATTTTTGCTG+TGG - chr1_3:24628601-24628620 Msa0111120:intron 30.0%
!! TTTTAGTCCATTTTTGCTGT+GGG - chr1_3:24628602-24628621 Msa0111120:intron 30.0%
! TTAAAATTTTGCAGTTAGGG+AGG - chr1_3:24628655-24628674 Msa0111120:intron 30.0%
ATGTACTTTGATTGAGGATT+AGG - chr1_3:24628754-24628773 Msa0111120:five_prime_UTR 30.0%
TGTACTTTGATTGAGGATTA+GGG - chr1_3:24628755-24628774 Msa0111120:five_prime_UTR 30.0%
GTTCAGATAAGCATAATGAA+TGG + chr1_3:24628810-24628829 None:intergenic 30.0%
TGCTTATCTGAACTAATTCT+AGG - chr1_3:24628817-24628836 Msa0111120:five_prime_UTR 30.0%
!! AATTCTAGGTTGATGAATAG+TGG - chr1_3:24628831-24628850 Msa0111120:five_prime_UTR 30.0%
! GTGGTGTAGTAAAATTTTTG+AGG - chr1_3:24628850-24628869 Msa0111120:five_prime_UTR 30.0%
! TCAGTCTCTCAATTCTCTTT+TGG - chr1_3:24625964-24625983 Msa0111120:three_prime_UTR 35.0%
! CAGTCTCTCAATTCTCTTTT+GGG - chr1_3:24625965-24625984 Msa0111120:three_prime_UTR 35.0%
! AGTCTCTCAATTCTCTTTTG+GGG - chr1_3:24625966-24625985 Msa0111120:three_prime_UTR 35.0%
GAAGGAATAATGAAATGGGT+TGG - chr1_3:24626268-24626287 Msa0111120:three_prime_UTR 35.0%
AATGGTGAAGAGAGTCAAAA+TGG + chr1_3:24626304-24626323 None:intergenic 35.0%
TGTCTTAGTGAGTGAAAATG+TGG - chr1_3:24626458-24626477 Msa0111120:CDS 35.0%
AATAAGCCGTTTATCCAAAC+AGG + chr1_3:24626607-24626626 None:intergenic 35.0%
! GTAAGTTTTGCGAGACTATT+AGG + chr1_3:24626817-24626836 None:intergenic 35.0%
TTGGATTTCTCATCTGACAT+AGG - chr1_3:24627306-24627325 Msa0111120:intron 35.0%
GCCAATACATTGATGCTTAA+AGG + chr1_3:24627645-24627664 None:intergenic 35.0%
! AGCATCAATGTATTGGCATT+TGG - chr1_3:24627648-24627667 Msa0111120:intron 35.0%
TACTTGTGATCACTGTTTCA+TGG - chr1_3:24627737-24627756 Msa0111120:intron 35.0%
TCCTGGAACATGAAAATAAG+CGG + chr1_3:24627906-24627925 None:intergenic 35.0%
!! TCTTTGGAAGTTTTGGTACT+CGG + chr1_3:24628221-24628240 None:intergenic 35.0%
! CATGCATTACAAAGTGTCTT+TGG + chr1_3:24628332-24628351 None:intergenic 35.0%
AAGACACTTTGTAATGCATG+TGG - chr1_3:24628332-24628351 Msa0111120:intron 35.0%
AGACACTTTGTAATGCATGT+GGG - chr1_3:24628333-24628352 Msa0111120:intron 35.0%
CATTTAGCATGCCAAAAGTT+AGG - chr1_3:24628536-24628555 Msa0111120:intron 35.0%
! CATGCCAAAAGTTAGGATTT+TGG - chr1_3:24628543-24628562 Msa0111120:intron 35.0%
AAAACATCCCACAGCAAAAA+TGG + chr1_3:24628612-24628631 None:intergenic 35.0%
! TCATTTGACTGTTTTCATGC+TGG - chr1_3:24628727-24628746 Msa0111120:intron 35.0%
GGTAACATGTACTTTGATTG+AGG - chr1_3:24628748-24628767 Msa0111120:five_prime_UTR 35.0%
AGTACACAAACGTGAAAAGC+TGG - chr1_3:24626058-24626077 Msa0111120:three_prime_UTR 40.0%
CAAGCAAGAAAAAGAGGGAA+AGG + chr1_3:24626150-24626169 None:intergenic 40.0%
! AGTGGGTGTGATAGTATTGA+AGG - chr1_3:24626250-24626269 Msa0111120:three_prime_UTR 40.0%
!! GGAATAATGAAATGGGTTGG+TGG - chr1_3:24626271-24626290 Msa0111120:three_prime_UTR 40.0%
!! TAATCTTTTCTGACTAGCCG+CGG - chr1_3:24626580-24626599 Msa0111120:CDS 40.0%
TGTCAGATGAGAAATCCAAG+AGG + chr1_3:24627305-24627324 None:intergenic 40.0%
TTTCTCATCTGACATAGGTG+AGG - chr1_3:24627311-24627330 Msa0111120:intron 40.0%
AGTGAAGGAAAGGCTTTCTT+AGG + chr1_3:24627354-24627373 None:intergenic 40.0%
!! TTCTGGTTTGAGTGAAGGAA+AGG + chr1_3:24627364-24627383 None:intergenic 40.0%
TGAATTGATGGGATCATCCA+GGG + chr1_3:24627418-24627437 None:intergenic 40.0%
TGAATGCCTGAGTTTACTTG+AGG - chr1_3:24627846-24627865 Msa0111120:intron 40.0%
GGAATTCCTCAAGTAAACTC+AGG + chr1_3:24627855-24627874 None:intergenic 40.0%
!! GCTTATTTTCATGTTCCAGG+AGG - chr1_3:24627905-24627924 Msa0111120:intron 40.0%
! TATTTTCATGTTCCAGGAGG+AGG - chr1_3:24627908-24627927 Msa0111120:intron 40.0%
GTCCAACAAAGATGCATTTC+CGG - chr1_3:24627956-24627975 Msa0111120:intron 40.0%
TCAACAAACGTTTCAACAGC+CGG + chr1_3:24627978-24627997 None:intergenic 40.0%
ACTTAAGAGGGTAATGCTGT+TGG + chr1_3:24628093-24628112 None:intergenic 40.0%
AACAGCATTACCCTCTTAAG+TGG - chr1_3:24628092-24628111 Msa0111120:intron 40.0%
GTGATTGTAGCCACTTAAGA+GGG + chr1_3:24628105-24628124 None:intergenic 40.0%
TGTGATTGTAGCCACTTAAG+AGG + chr1_3:24628106-24628125 None:intergenic 40.0%
TGAAACAAACCCCAACCATT+GGG - chr1_3:24628246-24628265 Msa0111120:intron 40.0%
ATGATGACATTTCCTCCCAA+TGG + chr1_3:24628264-24628283 None:intergenic 40.0%
!! GAGTTGATGATACTCCACAA+TGG - chr1_3:24628288-24628307 Msa0111120:intron 40.0%
! CAAAGTGTCTTTGGACCATT+TGG + chr1_3:24628323-24628342 None:intergenic 40.0%
GACACTTTGTAATGCATGTG+GGG - chr1_3:24628334-24628353 Msa0111120:intron 40.0%
TTTGTAATGCATGTGGGGTT+AGG - chr1_3:24628339-24628358 Msa0111120:intron 40.0%
ACATGCATTCTAATTCCCAC+AGG - chr1_3:24628423-24628442 Msa0111120:intron 40.0%
TAATTCCCACAGGAAAGTAG+TGG - chr1_3:24628433-24628452 Msa0111120:intron 40.0%
!! TTGCTGTGGGATGTTTTAGA+TGG - chr1_3:24628615-24628634 Msa0111120:intron 40.0%
!! TGCTGTGGGATGTTTTAGAT+GGG - chr1_3:24628616-24628635 Msa0111120:intron 40.0%
CTGAAAGAACCAACGAGAGA+TGG + chr1_3:24625948-24625967 None:intergenic 45.0%
!! AATCTTTTCTGACTAGCCGC+GGG - chr1_3:24626581-24626600 Msa0111120:CDS 45.0%
!! CTGCATTCTGGTTTGAGTGA+AGG + chr1_3:24627369-24627388 None:intergenic 45.0%
GTGAATTGATGGGATCATCC+AGG + chr1_3:24627419-24627438 None:intergenic 45.0%
GTTTCATGGTTTCCATGTCG+TGG - chr1_3:24627751-24627770 Msa0111120:intron 45.0%
!! GCCGCTTATTTTCATGTTCC+AGG - chr1_3:24627902-24627921 Msa0111120:intron 45.0%
TTGCAGAAGAGGAGCTTGAA+TGG - chr1_3:24627931-24627950 Msa0111120:intron 45.0%
AGCCGGAAATGCATCTTTGT+TGG + chr1_3:24627961-24627980 None:intergenic 45.0%
TGAGTTGTCCTGCATTCAAC+CGG - chr1_3:24627995-24628014 Msa0111120:intron 45.0%
CAAAAGATCCGGTTGAATGC+AGG + chr1_3:24628006-24628025 None:intergenic 45.0%
! TTGTGCTGTTCTCGAGCTTT+GGG + chr1_3:24628046-24628065 None:intergenic 45.0%
! GTTGTTTCCACGGGAATTGT+AGG + chr1_3:24628199-24628218 None:intergenic 45.0%
! TTGGTACTCGGTTGTTTCCA+CGG + chr1_3:24628209-24628228 None:intergenic 45.0%
GTGAAACAAACCCCAACCAT+TGG - chr1_3:24628245-24628264 Msa0111120:intron 45.0%
ACATTTCCTCCCAATGGTTG+GGG + chr1_3:24628258-24628277 None:intergenic 45.0%
GACATTTCCTCCCAATGGTT+GGG + chr1_3:24628259-24628278 None:intergenic 45.0%
TGACATTTCCTCCCAATGGT+TGG + chr1_3:24628260-24628279 None:intergenic 45.0%
GGGAGGAAATGTCATCATTG+TGG - chr1_3:24628266-24628285 Msa0111120:intron 45.0%
TGTGGGGTTAGGTATAAGTC+TGG - chr1_3:24628350-24628369 Msa0111120:intron 45.0%
GACGGTATTCAGGTACAAGT+CGG + chr1_3:24628376-24628395 None:intergenic 45.0%
CTATTTGCAGGACGGTATTC+AGG + chr1_3:24628386-24628405 None:intergenic 45.0%
AAGTTGGGCTATTTGCAGGA+CGG + chr1_3:24628394-24628413 None:intergenic 45.0%
CGAAAAGTTGGGCTATTTGC+AGG + chr1_3:24628398-24628417 None:intergenic 45.0%
GCATGTCACTGCGAAAAGTT+GGG + chr1_3:24628409-24628428 None:intergenic 45.0%
TGCATGTCACTGCGAAAAGT+TGG + chr1_3:24628410-24628429 None:intergenic 45.0%
CATCTCCACTACTTTCCTGT+GGG + chr1_3:24628441-24628460 None:intergenic 45.0%
TCATCTCCACTACTTTCCTG+TGG + chr1_3:24628442-24628461 None:intergenic 45.0%
! ACAGGAAAGTAGTGGAGATG+AGG - chr1_3:24628441-24628460 Msa0111120:intron 45.0%
ATGAGGAAGCAGAAGGGAAT+GGG - chr1_3:24628458-24628477 Msa0111120:intron 45.0%
! TTTTCAGAGATGGAGGCCCT+TGG - chr1_3:24627267-24627286 Msa0111120:intron 50.0%
GAAGGTGGATCACTGCATTC+TGG + chr1_3:24627381-24627400 None:intergenic 50.0%
TCCACCTTCGCTCAATCCAT+TGG - chr1_3:24627392-24627411 Msa0111120:CDS 50.0%
TCCGAGATTGTTAGTGCCTG+CGG - chr1_3:24627674-24627693 Msa0111120:intron 50.0%
ACCGCAGGCACTAACAATCT+CGG + chr1_3:24627678-24627697 None:intergenic 50.0%
! TGTGCTGTTCTCGAGCTTTG+GGG + chr1_3:24628045-24628064 None:intergenic 50.0%
! CTTGTGCTGTTCTCGAGCTT+TGG + chr1_3:24628047-24628066 None:intergenic 50.0%
! GTAGGTTTGAGGCATCAGCA+AGG + chr1_3:24628181-24628200 None:intergenic 50.0%
CCACGGGAATTGTAGGTTTG+AGG + chr1_3:24628192-24628211 None:intergenic 50.0%
CCTCAAACCTACAATTCCCG+TGG - chr1_3:24628189-24628208 Msa0111120:intron 50.0%
! TGGTACTCGGTTGTTTCCAC+GGG + chr1_3:24628208-24628227 None:intergenic 50.0%
AACAAACCCCAACCATTGGG+AGG - chr1_3:24628249-24628268 Msa0111120:intron 50.0%
AGTGGAGATGAGGAAGCAGA+AGG - chr1_3:24628451-24628470 Msa0111120:intron 50.0%
GTGGAGATGAGGAAGCAGAA+GGG - chr1_3:24628452-24628471 Msa0111120:intron 50.0%
GATGAGGAAGCAGAAGGGAA+TGG - chr1_3:24628457-24628476 Msa0111120:intron 50.0%
GAAGCAGAAGGGAATGGGAA+CGG - chr1_3:24628463-24628482 Msa0111120:intron 50.0%
AAGCAGAAGGGAATGGGAAC+GGG - chr1_3:24628464-24628483 Msa0111120:intron 50.0%
CTCTCTTGCCTGCTACTAGC+TGG - chr1_3:24626019-24626038 Msa0111120:three_prime_UTR 55.0%
GACAAGAGCCAGCTAGTAGC+AGG + chr1_3:24626030-24626049 None:intergenic 55.0%
CGTTTATCCAAACAGGCCCG+CGG + chr1_3:24626600-24626619 None:intergenic 55.0%
CGCGGGCCTGTTTGGATAAA+CGG - chr1_3:24626598-24626617 Msa0111120:CDS 55.0%
GAGGTCGTCCACAGAACCAA+GGG + chr1_3:24627286-24627305 None:intergenic 55.0%
AGAGGTCGTCCACAGAACCA+AGG + chr1_3:24627287-24627306 None:intergenic 55.0%
! TGGTTCTGTGGACGACCTCT+TGG - chr1_3:24627287-24627306 Msa0111120:intron 55.0%
!! GCCAATGGATTGAGCGAAGG+TGG + chr1_3:24627396-24627415 None:intergenic 55.0%
!! AGGGCCAATGGATTGAGCGA+AGG + chr1_3:24627399-24627418 None:intergenic 55.0%
TTCGCTCAATCCATTGGCCC+TGG - chr1_3:24627398-24627417 Msa0111120:CDS 55.0%
ATGGGATCATCCAGGGCCAA+TGG + chr1_3:24627411-24627430 None:intergenic 55.0%
! GAATCTGTCGTTTGCACCGC+AGG + chr1_3:24627693-24627712 None:intergenic 55.0%
AGAGATGCAGCTCCACGACA+TGG + chr1_3:24627766-24627785 None:intergenic 55.0%
TGAAGTTCCCTGTCCGTGCA+CGG - chr1_3:24628126-24628145 Msa0111120:intron 55.0%
! TACGAGACTTGCTCCGTGCA+CGG + chr1_3:24628142-24628161 None:intergenic 55.0%
! TTGAGGCATCAGCAAGGCCA+AGG + chr1_3:24628175-24628194 None:intergenic 55.0%
GATACTCCACAATGGCGTGC+AGG - chr1_3:24628296-24628315 Msa0111120:intron 55.0%
TTTGGACCTGCACGCCATTG+TGG + chr1_3:24628305-24628324 None:intergenic 55.0%
CAATGGCGTGCAGGTCCAAA+TGG - chr1_3:24628305-24628324 Msa0111120:intron 55.0%
!! GATGGAGGCCCTTGGTTCTG+TGG - chr1_3:24627275-24627294 Msa0111120:intron 60.0%
CCTCTTCTGCAACCTCCTCC+TGG + chr1_3:24627923-24627942 None:intergenic 60.0%
CCAGGAGGAGGTTGCAGAAG+AGG - chr1_3:24627920-24627939 Msa0111120:intron 60.0%
ACTTGCTCCGTGCACGGACA+GGG + chr1_3:24628136-24628155 None:intergenic 60.0%
TCTCGTAGCAAGCCTCGCCT+TGG - chr1_3:24628155-24628174 Msa0111120:intron 60.0%
! TGACTAGCCGCGGGCCTGTT+TGG - chr1_3:24626590-24626609 Msa0111120:CDS 65.0%
! GACTTGCTCCGTGCACGGAC+AGG + chr1_3:24628137-24628156 None:intergenic 65.0%
GCATCAGCAAGGCCAAGGCG+AGG + chr1_3:24628170-24628189 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 24625942 24628878 24625942 ID=Msa0111120;Name=Msa0111120
chr1_3 mRNA 24625942 24628878 24625942 ID=Msa0111120-mRNA-1;Parent=Msa0111120;Name=Msa0111120-mRNA-1;_AED=0.07;_eAED=0.07;_QI=140|1|1|1|0.5|0.66|3|388|244
chr1_3 exon 24625942 24626896 24625942 ID=Msa0111120-mRNA-1:exon:8478;Parent=Msa0111120-mRNA-1
chr1_3 exon 24627377 24627546 24627377 ID=Msa0111120-mRNA-1:exon:8477;Parent=Msa0111120-mRNA-1
chr1_3 exon 24628741 24628878 24628741 ID=Msa0111120-mRNA-1:exon:8476;Parent=Msa0111120-mRNA-1
chr1_3 five_prime_UTR 24628741 24628878 24628741 ID=Msa0111120-mRNA-1:five_prime_utr;Parent=Msa0111120-mRNA-1
chr1_3 five_prime_UTR 24627545 24627546 24627545 ID=Msa0111120-mRNA-1:five_prime_utr;Parent=Msa0111120-mRNA-1
chr1_3 CDS 24627377 24627544 24627377 ID=Msa0111120-mRNA-1:cds;Parent=Msa0111120-mRNA-1
chr1_3 CDS 24626330 24626896 24626330 ID=Msa0111120-mRNA-1:cds;Parent=Msa0111120-mRNA-1
chr1_3 three_prime_UTR 24625942 24626329 24625942 ID=Msa0111120-mRNA-1:three_prime_utr;Parent=Msa0111120-mRNA-1
Gene Sequence

>Msa0111120

ATGGAGGCCCTTGGTTCTGTGGACGACCTCTTGGATTTCTCATCTGACATAGGTGAGGATGATGATGATGACAAACCTAAGAAAGCCTTTCCTTCACTCAAACCAGAATGCAGTGATCCACCTTCGCTCAATCCATTGGCCCTGGATGATCCCATCAATTCACTTTCTGAGGAGGTTGCAGAAGAGGAGCTTGAATGGCTGTCCAACAAAGATGCATTTCCGGCTGTTGAAACGTTTGTTGAGTTGTCCTGCATTCAACCGGATCTTTTGAAGCATCAAATGACATCCCCAAAGCTCGAGAACAGCACAAGCAGCAGCAACAGCAATAACAGTTCCAACAGCATTACCCTCTTAAGTGGCTACAATCACATGAAGTTCCCTGTCCGTGCACGGAGCAAGTCTCGTAGCAAGCCTCGCCTTGGCCTTGCTGATGCCTCAAACCTACAATTCCCGTGGAAACAACCGAGTACCAAAACTTCCAAAGAAAAAGTGAAACAAACCCCAACCATTGGGAGGAAATGTCATCATTGTGGAGTTGATGATACTCCACAATGGCGTGCAGGTCCAAATGGTCCAAAGACACTTTGTAATGCATGTGGGGTTAGGTATAAGTCTGGCCGACTTGTACCTGAATACCGTCCTGCAAATAGCCCAACTTTTCGCAGTGACATGCATTCTAATTCCCACAGGAAAGTAGTGGAGATGAGGAAGCAGAAGGGAATGGGAACGGGTTGA
Protein sequence

>Msa0111120

MEALGSVDDLLDFSSDIGEDDDDDKPKKAFPSLKPECSDPPSLNPLALDDPINSLSEEVAEEELEWLSNKDAFPAVETFVELSCIQPDLLKHQMTSPKLENSTSSSNSNNSSNSITLLSGYNHMKFPVRARSKSRSKPRLGLADASNLQFPWKQPSTKTSKEKVKQTPTIGRKCHHCGVDDTPQWRAGPNGPKTLCNACGVRYKSGRLVPEYRPANSPTFRSDMHSNSHRKVVEMRKQKGMGTG*