AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0112650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0112650 MtrunA17_Chr1g0170581 78.431 204 12 2 3 174 44 247 1.89e-105 302
Msa0112650 MtrunA17_Chr1g0170571 77.114 201 14 2 3 171 36 236 3.60e-102 293
Msa0112650 MtrunA17_Chr1g0170591 73.134 201 22 2 3 171 36 236 7.95e-97 280
Msa0112650 MtrunA17_Chr7g0256671 57.778 180 42 4 22 168 64 242 6.48e-49 159
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0112650 AT3G56970.1 43.889 180 65 4 22 167 70 247 6.90e-37 128
Msa0112650 AT5G04150.2 40.741 162 68 3 19 152 78 239 3.29e-30 110
Msa0112650 AT5G04150.1 40.625 160 67 3 19 150 78 237 7.76e-30 110
Msa0112650 AT3G56980.1 43.407 182 67 4 22 169 75 254 2.61e-29 108
Msa0112650 AT2G41240.1 39.665 179 77 2 22 169 60 238 4.05e-28 105
Msa0112650 AT2G41240.2 39.106 179 77 3 22 169 60 237 1.05e-25 99.0

Find 34 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCACCTGTTTCTGTAATTC+TGG 0.155913 1_3:-27783347 None:intergenic
TGTAACTATGCTTCTTCCTT+TGG 0.251062 1_3:-27783448 None:intergenic
CTTCTATAATTTGCATTTCC+AGG 0.265122 1_3:+27783740 Msa0112650:CDS
GTAACTATGCTTCTTCCTTT+GGG 0.307422 1_3:-27783447 None:intergenic
TGAAAAGCTCTTATCAATAA+TGG 0.319674 1_3:+27784320 Msa0112650:CDS
CTTTCTTCATCTCAGACCTT+TGG 0.339976 1_3:+27783708 Msa0112650:intron
AGGACTCTTGAAATTGTTGC+TGG 0.367920 1_3:-27783314 None:intergenic
TAGAATAACCTCTTCTACTT+GGG 0.395863 1_3:-27783414 None:intergenic
TCTTCATCTCAGACCTTTGG+AGG 0.399010 1_3:+27783711 Msa0112650:intron
AGTACCATTTGATCTGAAAC+AGG 0.414053 1_3:-27783189 None:intergenic
CATACCAGAATTACAGAAAC+AGG 0.417584 1_3:+27783343 Msa0112650:CDS
TCTTCCTGTTTCAGATCAAA+TGG 0.439923 1_3:+27783185 Msa0112650:CDS
TTAGAATAACCTCTTCTACT+TGG 0.448901 1_3:-27783415 None:intergenic
CATGCCAAGGTCACTGCTTG+TGG 0.499479 1_3:-27783082 None:intergenic
ATCAATAATGGAGAAGGAAG+GGG 0.526977 1_3:+27784332 Msa0112650:CDS
ACAAATTATTGATCTTCTTG+CGG 0.529574 1_3:-27783143 None:intergenic
GCTCTTATCAATAATGGAGA+AGG 0.545227 1_3:+27784326 Msa0112650:CDS
CTTCATCTCAGACCTTTGGA+GGG 0.548412 1_3:+27783712 Msa0112650:intron
AAGAAGCATAGTTACAACTC+TGG 0.551749 1_3:+27783455 Msa0112650:CDS
TATCAATAATGGAGAAGGAA+GGG 0.552099 1_3:+27784331 Msa0112650:CDS
AGTGTAACATGAAATCTGAA+TGG 0.555088 1_3:-27783519 None:intergenic
ACTTTCTCCCCAAGTAGAAG+AGG 0.556979 1_3:+27783406 Msa0112650:CDS
TTATCAATAATGGAGAAGGA+AGG 0.562276 1_3:+27784330 Msa0112650:CDS
CAATCCACAAGCAGTGACCT+TGG 0.569748 1_3:+27783078 Msa0112650:CDS
AGAATAACCTCTTCTACTTG+GGG 0.586846 1_3:-27783413 None:intergenic
ACTAGTTAAGAGAAAAGAAG+AGG 0.589176 1_3:+27783373 Msa0112650:CDS
GTCACTGCTTGTGGATTGTG+AGG 0.591686 1_3:-27783073 None:intergenic
ATAGAAGACTCTCCCTCCAA+AGG 0.594272 1_3:-27783724 None:intergenic
CACAAGCAGTGACCTTGGCA+TGG 0.602895 1_3:+27783083 Msa0112650:CDS
TTCTAAGCAAAGAATCCCAA+AGG 0.620328 1_3:+27783432 Msa0112650:CDS
CAAGCTTCTTAACCATGCCA+AGG 0.655760 1_3:-27783095 None:intergenic
ACCAGAATTACAGAAACAGG+TGG 0.656125 1_3:+27783346 Msa0112650:CDS
GAATTACAGAAACAGGTGGA+AGG 0.659206 1_3:+27783350 Msa0112650:CDS
TCACTGCTTGTGGATTGTGA+GGG 0.698792 1_3:-27783072 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTTTTAATGTTTATAAAT+TGG - chr1_3:27782129-27782148 None:intergenic 10.0%
!!! GAAAAAAAAAAGTATATTTT+TGG - chr1_3:27782175-27782194 None:intergenic 10.0%
!! AAAAAAGGTTATATATATAA+GGG + chr1_3:27782273-27782292 Msa0112650:intron 10.0%
!! ATAAAAAATTTAAGAGTTTA+AGG + chr1_3:27782670-27782689 Msa0112650:intron 10.0%
!!! TATAAGTAAATTTTTTTTGT+AGG + chr1_3:27782730-27782749 Msa0112650:intron 10.0%
!!! ATAAGTAAATTTTTTTTGTA+GGG + chr1_3:27782731-27782750 Msa0112650:intron 10.0%
!! ACTAAAAATATAAGAAATTA+TGG + chr1_3:27784129-27784148 Msa0112650:intron 10.0%
!!! TATTAATTTGTTCAGAAAAA+AGG + chr1_3:27782258-27782277 Msa0112650:intron 15.0%
!! GAAAAAAGGTTATATATATA+AGG + chr1_3:27782272-27782291 Msa0112650:intron 15.0%
!! AATGCTATTAAAATTAAGTA+AGG - chr1_3:27782760-27782779 None:intergenic 15.0%
!! AAGCATAAATTAAACAAATT+TGG - chr1_3:27782419-27782438 None:intergenic 15.0%
!!! ATTTTTGCGATATTTTTTTT+CGG - chr1_3:27782503-27782522 None:intergenic 15.0%
!! AGATATAAGTAACAAAAATA+TGG - chr1_3:27782539-27782558 None:intergenic 15.0%
!!! TAAACAAAATTTATTTTAGC+AGG + chr1_3:27782698-27782717 Msa0112650:intron 15.0%
!!! AAACAAAATTTATTTTAGCA+GGG + chr1_3:27782699-27782718 Msa0112650:intron 15.0%
!! AATGCTATTAAAATTAAGTA+AGG + chr1_3:27782760-27782779 Msa0112650:intron 15.0%
!! ATTAACAAAATCATATAGAA+GGG - chr1_3:27783223-27783242 None:intergenic 15.0%
!! AATTAACAAAATCATATAGA+AGG - chr1_3:27783224-27783243 None:intergenic 15.0%
!! GATCATATATAATTATGATT+TGG + chr1_3:27783911-27783930 Msa0112650:intron 15.0%
!! ATACAAAATAATTGATTACT+TGG + chr1_3:27784165-27784184 Msa0112650:intron 15.0%
!! TACAAAATAATTGATTACTT+GGG + chr1_3:27784166-27784185 Msa0112650:intron 15.0%
!! ATTATGAGTATTATCTAACT+TGG - chr1_3:27780807-27780826 None:intergenic 20.0%
!! TGATATGTATGAAATGATTA+TGG + chr1_3:27781254-27781273 Msa0112650:intron 20.0%
!! TACTTACAAGTATAGATTAA+AGG + chr1_3:27781296-27781315 Msa0112650:intron 20.0%
!! TTCTAGACACTATTAAATAT+TGG + chr1_3:27781770-27781789 Msa0112650:intron 20.0%
!! TCTAATAACATTAGTCAATA+GGG - chr1_3:27781992-27782011 None:intergenic 20.0%
!! ATCTAATAACATTAGTCAAT+AGG - chr1_3:27781993-27782012 None:intergenic 20.0%
!!! ATTAGATGTTACTTATCTTT+CGG + chr1_3:27782006-27782025 Msa0112650:intron 20.0%
!! ATTAGATGTCCTTAAAATTA+AGG + chr1_3:27782101-27782120 Msa0112650:intron 20.0%
!!! AAATTGGTTCCTTAATTTTA+AGG - chr1_3:27782113-27782132 None:intergenic 20.0%
!!! AGTGAAATACTATATTTTGT+TGG + chr1_3:27782147-27782166 Msa0112650:intron 20.0%
!!! GTGAAATACTATATTTTGTT+GGG + chr1_3:27782148-27782167 Msa0112650:intron 20.0%
!! GTGTTAAGAACATTATTAAA+AGG + chr1_3:27782473-27782492 Msa0112650:intron 20.0%
!!! TATAAGGTTAGTTATGTTTT+TGG - chr1_3:27782818-27782837 None:intergenic 20.0%
!! CTAACCTTATATATAAATGT+TGG + chr1_3:27782827-27782846 Msa0112650:intron 20.0%
!! CTATCCAACATTTATATATA+AGG - chr1_3:27782834-27782853 None:intergenic 20.0%
!! ATATTTCGTCAATTATGAAT+TGG + chr1_3:27783800-27783819 Msa0112650:intron 20.0%
!! TTAATAACATGTAAGAAGTA+AGG + chr1_3:27784105-27784124 Msa0112650:intron 20.0%
! TCACTAGAAAGTCTTAAAAT+CGG - chr1_3:27781567-27781586 None:intergenic 25.0%
! AAAATAATGTAACGTATGAC+TGG + chr1_3:27781656-27781675 Msa0112650:intron 25.0%
! ATAAATACGAGCGTTATAAT+AGG - chr1_3:27781748-27781767 None:intergenic 25.0%
! TAAATGACATGAAATCTACA+CGG - chr1_3:27782055-27782074 None:intergenic 25.0%
!! CCTATATTTTATTGTTAGGA+AGG - chr1_3:27782197-27782216 None:intergenic 25.0%
! CCTTCCTAACAATAAAATAT+AGG + chr1_3:27782194-27782213 Msa0112650:intron 25.0%
!! GCATCCTATATTTTATTGTT+AGG - chr1_3:27782201-27782220 None:intergenic 25.0%
! AAACAAATTTGGATTGAGTA+AGG - chr1_3:27782408-27782427 None:intergenic 25.0%
!!! GTTACTCAACAATTTTTTAC+AGG + chr1_3:27782449-27782468 Msa0112650:intron 25.0%
! AAGTAAGGACTAAATTTACA+AGG + chr1_3:27782775-27782794 Msa0112650:intron 25.0%
!!! GAAAACAGTTTTTTTTAGAC+TGG - chr1_3:27782888-27782907 None:intergenic 25.0%
!!! TAATTTTCATGATCAAGTTG+AGG + chr1_3:27783029-27783048 Msa0112650:intron 25.0%
! ACAAATTATTGATCTTCTTG+CGG - chr1_3:27783146-27783165 None:intergenic 25.0%
!! GTAATTCTGGTATGTATTTT+AGG - chr1_3:27783337-27783356 None:intergenic 25.0%
! TTGATATGTTTGGAAAATGA+TGG + chr1_3:27783669-27783688 Msa0112650:intron 25.0%
! AAGAAAGATTAAGAAGCAAA+AGG - chr1_3:27783695-27783714 None:intergenic 25.0%
!!! ACACAAAGAGCTTTTAATTA+AGG - chr1_3:27784268-27784287 None:intergenic 25.0%
! TGAAAAGCTCTTATCAATAA+TGG + chr1_3:27784320-27784339 Msa0112650:CDS 25.0%
CACATAAATTTAGCAAGAGT+TGG + chr1_3:27780863-27780882 Msa0112650:intron 30.0%
GCATATGAATGAAATGTTGT+AGG + chr1_3:27781018-27781037 Msa0112650:intron 30.0%
TATGTATGGGATTTGTACTT+GGG + chr1_3:27781170-27781189 Msa0112650:intron 30.0%
! AATTATCCGAGTTCGTTTTA+CGG - chr1_3:27781216-27781235 None:intergenic 30.0%
TATGGCAAGCAAATAATGAA+AGG + chr1_3:27781272-27781291 Msa0112650:intron 30.0%
TACACGTATAATCAACCAAT+TGG + chr1_3:27781365-27781384 Msa0112650:intron 30.0%
ACCTGAACAAATGAAAGAAT+GGG - chr1_3:27781483-27781502 None:intergenic 30.0%
AACCTGAACAAATGAAAGAA+TGG - chr1_3:27781484-27781503 None:intergenic 30.0%
AAAGATTGTCGTATGAAACT+TGG - chr1_3:27781633-27781652 None:intergenic 30.0%
TATTCAACTACTACATTGTG+TGG - chr1_3:27781851-27781870 None:intergenic 30.0%
GTTATAACTAAACTATCCAG+CGG + chr1_3:27781879-27781898 Msa0112650:intron 30.0%
AAGTAAGGACTAAACTTACA+AGG - chr1_3:27782332-27782351 None:intergenic 30.0%
!!! TTTGCGATATTTTTTTTCGG+AGG - chr1_3:27782500-27782519 None:intergenic 30.0%
! CAAGTTTAGAACACTATGAA+AGG - chr1_3:27782636-27782655 None:intergenic 30.0%
TATCGCTAACATAATGTTGA+TGG - chr1_3:27782918-27782937 None:intergenic 30.0%
CATCCAATTGTTGAAAACAT+AGG - chr1_3:27782947-27782966 None:intergenic 30.0%
! CTATGTTTTCAACAATTGGA+TGG + chr1_3:27782945-27782964 Msa0112650:intron 30.0%
ACTAGTTAAGAGAAAAGAAG+AGG + chr1_3:27783373-27783392 Msa0112650:CDS 30.0%
TAGAATAACCTCTTCTACTT+GGG - chr1_3:27783417-27783436 None:intergenic 30.0%
TTAGAATAACCTCTTCTACT+TGG - chr1_3:27783418-27783437 None:intergenic 30.0%
! GTTTTGTAGTTTCAACAAGT+AGG + chr1_3:27783477-27783496 Msa0112650:CDS 30.0%
AGTGTAACATGAAATCTGAA+TGG - chr1_3:27783522-27783541 None:intergenic 30.0%
!! TTCTGAGATCTTGATATGTT+TGG + chr1_3:27783659-27783678 Msa0112650:intron 30.0%
! CTTCTATAATTTGCATTTCC+AGG + chr1_3:27783740-27783759 Msa0112650:CDS 30.0%
AAGATGTAGAGTCATATAAG+AGG + chr1_3:27784227-27784246 Msa0112650:intron 30.0%
! TTATCAATAATGGAGAAGGA+AGG + chr1_3:27784330-27784349 Msa0112650:CDS 30.0%
! TATCAATAATGGAGAAGGAA+GGG + chr1_3:27784331-27784350 Msa0112650:CDS 30.0%
! TACATGTTTTCCCAACCATT+AGG - chr1_3:27780957-27780976 None:intergenic 35.0%
CTATGTATGGGATTTGTACT+TGG + chr1_3:27781169-27781188 Msa0112650:intron 35.0%
ATGTATGGGATTTGTACTTG+GGG + chr1_3:27781171-27781190 Msa0112650:intron 35.0%
AACAATCCGTAAAACGAACT+CGG + chr1_3:27781207-27781226 Msa0112650:intron 35.0%
GTGTCTACATTACAACCAAT+TGG - chr1_3:27781383-27781402 None:intergenic 35.0%
!! ATAACTTTGGGGACATTTTG+TGG - chr1_3:27781413-27781432 None:intergenic 35.0%
TCCCATTCTTTCATTTGTTC+AGG + chr1_3:27781479-27781498 Msa0112650:intron 35.0%
! TTTCATTTGTTCAGGTTCAC+AGG + chr1_3:27781487-27781506 Msa0112650:intron 35.0%
! TACAAGGTCGCAATTTTGTA+GGG - chr1_3:27782791-27782810 None:intergenic 35.0%
! TTACAAGGTCGCAATTTTGT+AGG - chr1_3:27782790-27782809 None:intergenic 35.0%
!!! GAGTGCAGAATTTTTTTGCA+GGG - chr1_3:27782376-27782395 None:intergenic 35.0%
!!! TGAGTGCAGAATTTTTTTGC+AGG - chr1_3:27782377-27782396 None:intergenic 35.0%
! TTACAAGGTCGCAATTTTGT+AGG + chr1_3:27782790-27782809 Msa0112650:intron 35.0%
! TACAAGGTCGCAATTTTGTA+GGG + chr1_3:27782791-27782810 Msa0112650:intron 35.0%
CCAATTGTTGAAAACATAGG+AGG - chr1_3:27782944-27782963 None:intergenic 35.0%
! CCTCCTATGTTTTCAACAAT+TGG + chr1_3:27782941-27782960 Msa0112650:intron 35.0%
! CTTTGTAGAATGCTGGTTAT+GGG - chr1_3:27782990-27783009 None:intergenic 35.0%
! TCTTTGTAGAATGCTGGTTA+TGG - chr1_3:27782991-27783010 None:intergenic 35.0%
TCTTCCTGTTTCAGATCAAA+TGG + chr1_3:27783185-27783204 Msa0112650:CDS 35.0%
! AGTACCATTTGATCTGAAAC+AGG - chr1_3:27783192-27783211 None:intergenic 35.0%
CATACCAGAATTACAGAAAC+AGG + chr1_3:27783343-27783362 Msa0112650:CDS 35.0%
AGAATAACCTCTTCTACTTG+GGG - chr1_3:27783416-27783435 None:intergenic 35.0%
TTCTAAGCAAAGAATCCCAA+AGG + chr1_3:27783432-27783451 Msa0112650:CDS 35.0%
GTAACTATGCTTCTTCCTTT+GGG - chr1_3:27783450-27783469 None:intergenic 35.0%
TGTAACTATGCTTCTTCCTT+TGG - chr1_3:27783451-27783470 None:intergenic 35.0%
! AAGAAGCATAGTTACAACTC+TGG + chr1_3:27783455-27783474 Msa0112650:CDS 35.0%
CATATCAAGATCTCAGAAAG+TGG - chr1_3:27783657-27783676 None:intergenic 35.0%
CATAGTGAAATTAACACACC+TGG - chr1_3:27783761-27783780 None:intergenic 35.0%
! GCTCTTATCAATAATGGAGA+AGG + chr1_3:27784326-27784345 Msa0112650:CDS 35.0%
! ATCAATAATGGAGAAGGAAG+GGG + chr1_3:27784332-27784351 Msa0112650:CDS 35.0%
! ATGAAATGTTGTAGGTGTCG+CGG + chr1_3:27781026-27781045 Msa0112650:intron 40.0%
!! TTCGTTTTACGGATTGTTCC+GGG - chr1_3:27781205-27781224 None:intergenic 40.0%
!! GTTCGTTTTACGGATTGTTC+CGG - chr1_3:27781206-27781225 None:intergenic 40.0%
CTAGGCTAGTGATAACTTTG+GGG - chr1_3:27781424-27781443 None:intergenic 40.0%
CCTAGGCTAGTGATAACTTT+GGG - chr1_3:27781425-27781444 None:intergenic 40.0%
TCCTAGGCTAGTGATAACTT+TGG - chr1_3:27781426-27781445 None:intergenic 40.0%
GTGAGTTCTATCTACTTCCT+AGG - chr1_3:27781442-27781461 None:intergenic 40.0%
CAAAGCCTTCTTCTTACCTT+TGG - chr1_3:27781522-27781541 None:intergenic 40.0%
CAAAGGTAAGAAGAAGGCTT+TGG + chr1_3:27781520-27781539 Msa0112650:intron 40.0%
TTGGATTGATCGTGCTATCA+TGG + chr1_3:27781539-27781558 Msa0112650:intron 40.0%
CAATACAGAGCGACATAAGT+CGG - chr1_3:27781705-27781724 None:intergenic 40.0%
ACGAGCGTTATAATAGGTAC+TGG - chr1_3:27781742-27781761 None:intergenic 40.0%
! AGTTCTAATCCTGTTGGAAG+AGG - chr1_3:27781799-27781818 None:intergenic 40.0%
! CAAGAGAGTTCTAATCCTGT+TGG - chr1_3:27781805-27781824 None:intergenic 40.0%
AGAGCTAACAAATTAACCGC+TGG - chr1_3:27781898-27781917 None:intergenic 40.0%
ATGACATGAAATCTACACGG+CGG - chr1_3:27782052-27782071 None:intergenic 40.0%
AATATAGGATGCTTGCGTAG+AGG + chr1_3:27782209-27782228 Msa0112650:intron 40.0%
ATATAGGATGCTTGCGTAGA+GGG + chr1_3:27782210-27782229 Msa0112650:intron 40.0%
! GAATGCTGGTTATGGGTTAT+TGG - chr1_3:27782983-27783002 None:intergenic 40.0%
AGGACTCTTGAAATTGTTGC+TGG - chr1_3:27783317-27783336 None:intergenic 40.0%
ACCAGAATTACAGAAACAGG+TGG + chr1_3:27783346-27783365 Msa0112650:CDS 40.0%
!! TCCACCTGTTTCTGTAATTC+TGG - chr1_3:27783350-27783369 None:intergenic 40.0%
GAATTACAGAAACAGGTGGA+AGG + chr1_3:27783350-27783369 Msa0112650:CDS 40.0%
CTTTCTTCATCTCAGACCTT+TGG + chr1_3:27783708-27783727 Msa0112650:intron 40.0%
AGATTAGAGCCTCGACCTAA+TGG + chr1_3:27780939-27780958 Msa0112650:intron 45.0%
! TTTTCCCAACCATTAGGTCG+AGG - chr1_3:27780951-27780970 None:intergenic 45.0%
AGGGGTTTCCTTATGGGAAT+GGG + chr1_3:27781077-27781096 Msa0112650:intron 45.0%
GAGCTTATCCCATTCCCATA+AGG - chr1_3:27781088-27781107 None:intergenic 45.0%
GAATGGGATAAGCTCTGCAA+GGG + chr1_3:27781093-27781112 Msa0112650:intron 45.0%
AATGGGATAAGCTCTGCAAG+GGG + chr1_3:27781094-27781113 Msa0112650:intron 45.0%
! AGTACCTAGCTAAGGCATCT+CGG - chr1_3:27781144-27781163 None:intergenic 45.0%
ATGCCTTAGCTAGGTACTCT+CGG + chr1_3:27781146-27781165 Msa0112650:intron 45.0%
TAGGTACTCTCGGCTATGTA+TGG + chr1_3:27781156-27781175 Msa0112650:intron 45.0%
AGGTACTCTCGGCTATGTAT+GGG + chr1_3:27781157-27781176 Msa0112650:intron 45.0%
! CCCAAAGTTATCACTAGCCT+AGG + chr1_3:27781422-27781441 Msa0112650:intron 45.0%
GAGTGCCAAAGGTAAGAAGA+AGG + chr1_3:27781514-27781533 Msa0112650:intron 45.0%
TATTGGACACCTCTTCCAAC+AGG + chr1_3:27781787-27781806 Msa0112650:intron 45.0%
GGTACAAACACTACACAACG+TGG - chr1_3:27781830-27781849 None:intergenic 45.0%
AACAATTGGATGGCCCTTTG+AGG + chr1_3:27782955-27782974 Msa0112650:intron 45.0%
!! TGGGTTATTGGCTCCTCAAA+GGG - chr1_3:27782971-27782990 None:intergenic 45.0%
!! ATGGGTTATTGGCTCCTCAA+AGG - chr1_3:27782972-27782991 None:intergenic 45.0%
GCAGTGTCTTTGTAGAATGC+TGG - chr1_3:27782997-27783016 None:intergenic 45.0%
! TCACTGCTTGTGGATTGTGA+GGG - chr1_3:27783075-27783094 None:intergenic 45.0%
CAAGCTTCTTAACCATGCCA+AGG - chr1_3:27783098-27783117 None:intergenic 45.0%
ACTTTCTCCCCAAGTAGAAG+AGG + chr1_3:27783406-27783425 Msa0112650:CDS 45.0%
TCTTCATCTCAGACCTTTGG+AGG + chr1_3:27783711-27783730 Msa0112650:intron 45.0%
CTTCATCTCAGACCTTTGGA+GGG + chr1_3:27783712-27783731 Msa0112650:intron 45.0%
ATAGAAGACTCTCCCTCCAA+AGG - chr1_3:27783727-27783746 None:intergenic 45.0%
TAGAGCCTCGACCTAATGGT+TGG + chr1_3:27780943-27780962 Msa0112650:intron 50.0%
AGAGCCTCGACCTAATGGTT+GGG + chr1_3:27780944-27780963 Msa0112650:intron 50.0%
GATGCGAGGGGTTTCCTTAT+GGG + chr1_3:27781071-27781090 Msa0112650:intron 50.0%
GAGGGGTTTCCTTATGGGAA+TGG + chr1_3:27781076-27781095 Msa0112650:intron 50.0%
GGAATGGGATAAGCTCTGCA+AGG + chr1_3:27781092-27781111 Msa0112650:intron 50.0%
ATGGGATAAGCTCTGCAAGG+GGG + chr1_3:27781095-27781114 Msa0112650:intron 50.0%
!! TAGCCGAGAGTACCTAGCTA+AGG - chr1_3:27781152-27781171 None:intergenic 50.0%
!! GTTTTACGGATTGTTCCGGG+TGG - chr1_3:27781202-27781221 None:intergenic 50.0%
! TCACAGGTTTCGAGTGCCAA+AGG + chr1_3:27781503-27781522 Msa0112650:intron 50.0%
! GTAGAGGGTGCATAGCATGA+TGG + chr1_3:27782225-27782244 Msa0112650:intron 50.0%
GGGTGCATAGCATGATGGTT+AGG + chr1_3:27782230-27782249 Msa0112650:intron 50.0%
! GTCACTGCTTGTGGATTGTG+AGG - chr1_3:27783076-27783095 None:intergenic 50.0%
CAATCCACAAGCAGTGACCT+TGG + chr1_3:27783078-27783097 Msa0112650:CDS 50.0%
GGCTCTCGTCATCCACATCA+GGG + chr1_3:27781047-27781066 Msa0112650:intron 55.0%
GCTCTCGTCATCCACATCAG+GGG + chr1_3:27781048-27781067 Msa0112650:intron 55.0%
CTCTCGTCATCCACATCAGG+GGG + chr1_3:27781049-27781068 Msa0112650:intron 55.0%
GGATGCGAGGGGTTTCCTTA+TGG + chr1_3:27781070-27781089 Msa0112650:intron 55.0%
TGGGATAAGCTCTGCAAGGG+GGG + chr1_3:27781096-27781115 Msa0112650:intron 55.0%
GAGTCCGAGATGCCTTAGCT+AGG + chr1_3:27781137-27781156 Msa0112650:intron 55.0%
GTACTTGGGGTTACTCCACC+CGG + chr1_3:27781184-27781203 Msa0112650:intron 55.0%
TGCATACCTACACGACCGTG+CGG - chr1_3:27781336-27781355 None:intergenic 55.0%
CATGCCAAGGTCACTGCTTG+TGG - chr1_3:27783085-27783104 None:intergenic 55.0%
CACAAGCAGTGACCTTGGCA+TGG + chr1_3:27783083-27783102 Msa0112650:CDS 55.0%
CGGCTCTCGTCATCCACATC+AGG + chr1_3:27781046-27781065 Msa0112650:intron 60.0%
ATCCACATCAGGGGGATGCG+AGG + chr1_3:27781057-27781076 Msa0112650:intron 60.0%
TCCACATCAGGGGGATGCGA+GGG + chr1_3:27781058-27781077 Msa0112650:intron 60.0%
CATACCTACACGACCGTGCG+GGG - chr1_3:27781334-27781353 None:intergenic 60.0%
GCATACCTACACGACCGTGC+GGG - chr1_3:27781335-27781354 None:intergenic 60.0%
CCACATCAGGGGGATGCGAG+GGG + chr1_3:27781059-27781078 Msa0112650:intron 65.0%
GACTCCCTCAGTGCCGTGTG+GGG - chr1_3:27781122-27781141 None:intergenic 65.0%
GGACTCCCTCAGTGCCGTGT+GGG - chr1_3:27781123-27781142 None:intergenic 65.0%
GAGCGACCTAGTACCCCGCA+CGG + chr1_3:27781318-27781337 Msa0112650:intron 65.0%
! ACACGACCGTGCGGGGTACT+AGG - chr1_3:27781327-27781346 None:intergenic 65.0%
AGTACCCCGCACGGTCGTGT+AGG + chr1_3:27781327-27781346 Msa0112650:intron 65.0%
CCCCTCGCATCCCCCTGATG+TGG - chr1_3:27781062-27781081 None:intergenic 70.0%
TCTGCAAGGGGGGCCCCACA+CGG + chr1_3:27781106-27781125 Msa0112650:intron 70.0%
CGGACTCCCTCAGTGCCGTG+TGG - chr1_3:27781124-27781143 None:intergenic 70.0%
! GGGGCCCCACACGGCACTGA+GGG + chr1_3:27781115-27781134 Msa0112650:intron 75.0%
! GGGGGCCCCACACGGCACTG+AGG + chr1_3:27781114-27781133 Msa0112650:intron 80.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 27780801 27784371 27780801 ID=Msa0112650;Name=Msa0112650
chr1_3 mRNA 27780801 27784371 27780801 ID=Msa0112650-mRNA-1;Parent=Msa0112650;Name=Msa0112650-mRNA-1;_AED=0.26;_eAED=0.26;_QI=0|0|0|0.6|0.25|0.4|5|0|174
chr1_3 exon 27780801 27780807 27780801 ID=Msa0112650-mRNA-1:exon:9206;Parent=Msa0112650-mRNA-1
chr1_3 exon 27783046 27783206 27783046 ID=Msa0112650-mRNA-1:exon:9207;Parent=Msa0112650-mRNA-1
chr1_3 exon 27783299 27783541 27783299 ID=Msa0112650-mRNA-1:exon:9208;Parent=Msa0112650-mRNA-1
chr1_3 exon 27783723 27783761 27783723 ID=Msa0112650-mRNA-1:exon:9209;Parent=Msa0112650-mRNA-1
chr1_3 exon 27784297 27784371 27784297 ID=Msa0112650-mRNA-1:exon:9210;Parent=Msa0112650-mRNA-1
chr1_3 CDS 27780801 27780807 27780801 ID=Msa0112650-mRNA-1:cds;Parent=Msa0112650-mRNA-1
chr1_3 CDS 27783046 27783206 27783046 ID=Msa0112650-mRNA-1:cds;Parent=Msa0112650-mRNA-1
chr1_3 CDS 27783299 27783541 27783299 ID=Msa0112650-mRNA-1:cds;Parent=Msa0112650-mRNA-1
chr1_3 CDS 27783723 27783761 27783723 ID=Msa0112650-mRNA-1:cds;Parent=Msa0112650-mRNA-1
chr1_3 CDS 27784297 27784371 27784297 ID=Msa0112650-mRNA-1:cds;Parent=Msa0112650-mRNA-1
Gene Sequence

>Msa0112650

ATGCCAATTGAGGCAGAAATTAATTCAAAAGATCCCTCACAATCCACAAGCAGTGACCTTGGCATGGTTAAGAAGCTTGTTCATAATGCTAGTGAACGCGATCGCCGCAAGAAGATCAATAATTTGTATTCATCACTTCGTTCACTTCTTCCTGTTTCAGATCAAATGAAGAAATTGAGCATTCCAGCAACAATTTCAAGAGTCCTAAAATACATACCAGAATTACAGAAACAGGTGGAAGGACTAGTTAAGAGAAAAGAAGAGGTCTTATTGAGACTTTCTCCCCAAGTAGAAGAGGTTATTCTAAGCAAAGAATCCCAAAGGAAGAAGCATAGTTACAACTCTGGTTTTGTAGTTTCAACAAGTAGGCTCAATGATAGTGAAATTACCATTCAGATTTCATGTTACACTACCTTTGGAGGGAGAGTCTTCTATAATTTGCATTTCCAGAGATTAGAATCTCATATTCTAAATGAAAAGCTCTTATCAATAATGGAGAAGGAAGGGGAGTTTTTAAAACAATAA
Protein sequence

>Msa0112650

MPIEAEINSKDPSQSTSSDLGMVKKLVHNASERDRRKKINNLYSSLRSLLPVSDQMKKLSIPATISRVLKYIPELQKQVEGLVKRKEEVLLRLSPQVEEVILSKESQRKKHSYNSGFVVSTSRLNDSEITIQISCYTTFGGRVFYNLHFQRLESHILNEKLLSIMEKEGEFLKQ*