AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0113440

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0113440 MtrunA17_Chr1g0171291 90.141 284 12 2 1 284 1 268 2.45e-162 451
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0113440 AT1G42990.1 33.663 303 146 11 6 284 22 293 1.97e-14 72.4

Find 87 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTTCCCTGCTATGGTTCC+TGG 0.201475 1_3:+29441313 Msa0113440:CDS
TTCTTCCTATTTGAAATATA+TGG 0.218852 1_3:+29441764 Msa0113440:three_prime_UTR
ATCATCTTCTTCGTAATTTC+TGG 0.223093 1_3:+29439251 Msa0113440:five_prime_UTR
GAGAGATTCGAAATCGATTT+GGG 0.252626 1_3:-29439295 None:intergenic
CTAAGAAGGGTGGCTCTAAA+AGG 0.288472 1_3:+29441419 Msa0113440:CDS
CAAACATGTTGGGATGCTTT+TGG 0.296968 1_3:+29441698 Msa0113440:three_prime_UTR
TTGTGTTGTCTTAGTGATTA+AGG 0.313977 1_3:-29439141 None:intergenic
AGAGAGATTCGAAATCGATT+TGG 0.334822 1_3:-29439296 None:intergenic
CAAAAGCATCCCAACATGTT+TGG 0.338640 1_3:-29441697 None:intergenic
GGAGTCTGCTGTGCTCTTGT+TGG 0.367032 1_3:+29440075 Msa0113440:CDS
GGAACCTCTTGAGGAGTTAC+TGG 0.369486 1_3:-29439561 None:intergenic
TGATCATCCGATTGAGGGAT+TGG 0.372571 1_3:-29439504 None:intergenic
GGAACCTCTTGAGGAGTTAC+TGG 0.381189 1_3:-29439606 None:intergenic
AACTTCCATATATTTCAAAT+AGG 0.389868 1_3:-29441769 None:intergenic
TCATCTTCTTCGTAATTTCT+GGG 0.397678 1_3:+29439252 Msa0113440:five_prime_UTR
TTGCAATCGCGTGGTGGCTT+TGG 0.414223 1_3:+29440034 Msa0113440:CDS
TGCTGTTGGGTTCCCTGCTA+TGG 0.415075 1_3:+29441306 Msa0113440:CDS
TTTGCAGAATCCCTGCTGTT+GGG 0.426943 1_3:+29441293 Msa0113440:intron
GGTTCCCTGCTATGGTTCCT+GGG 0.440801 1_3:+29441314 Msa0113440:CDS
GAGTCTGCTGTGCTCTTGTT+GGG 0.447976 1_3:+29440076 Msa0113440:CDS
TCATCTTCGTTCTTGAAGCT+TGG 0.448828 1_3:-29441505 None:intergenic
TGATGCCCAGGAACCATAGC+AGG 0.462353 1_3:-29441319 None:intergenic
TCTTTACAAAAGATTGAGTC+AGG 0.468262 1_3:-29441472 None:intergenic
AACCTTGATCATCCGATTGA+GGG 0.476612 1_3:-29439509 None:intergenic
CCTCGACCCAATCCCTCAAT+CGG 0.479515 1_3:+29439497 Msa0113440:CDS
AGGTTCCTTCTGATGAAGGC+AGG 0.489060 1_3:+29439577 Msa0113440:CDS
ATATGTAAACCAAACATGTT+GGG 0.490186 1_3:+29441688 Msa0113440:three_prime_UTR
TGAAGGGGAATGCAGAAGAC+TGG 0.491226 1_3:+29439961 Msa0113440:CDS
GGGAATTGCGTCGTCATCAT+CGG 0.491346 1_3:+29439440 Msa0113440:CDS
GATCATCCGATTGAGGGATT+GGG 0.498027 1_3:-29439503 None:intergenic
AGCAGACTCCTGCATGGTCA+TGG 0.499805 1_3:-29440062 None:intergenic
CAAGAGGTTCCTTCTGATGA+AGG 0.500088 1_3:+29439573 Msa0113440:CDS
AATATGTAAACCAAACATGT+TGG 0.501452 1_3:+29441687 Msa0113440:three_prime_UTR
AGAGAAAATGCAAAGAAAGA+AGG 0.503069 1_3:-29441588 None:intergenic
ATGCTGAGAACCATGCTTTG+CGG 0.510527 1_3:+29440005 Msa0113440:CDS
AAACCTTGATCATCCGATTG+AGG 0.512112 1_3:-29439510 None:intergenic
CTGTTGTTAGCCACAGTGGA+AGG 0.514860 1_3:-29441361 None:intergenic
TGGAACACAAAGGTCTAAGA+AGG 0.519321 1_3:+29441405 Msa0113440:CDS
CCGATTGAGGGATTGGGTCG+AGG 0.520843 1_3:-29439497 None:intergenic
TGGTGCTCCCATGACCATGC+AGG 0.523079 1_3:+29440054 Msa0113440:CDS
TCTTCTCATGTCTGATGCCG+AGG 0.542387 1_3:+29439419 Msa0113440:CDS
AGGAGTTACTGGATCAACTC+TGG 0.544068 1_3:-29439550 None:intergenic
GTTGTTAGCCACAGTGGAAG+GGG 0.545341 1_3:-29441359 None:intergenic
ATCCCTCAATCGGATGATCA+AGG 0.545906 1_3:+29439507 Msa0113440:CDS
TTCGAGAAACTTATGATAGT+CGG 0.546362 1_3:-29439466 None:intergenic
TCAACTCTGGCTTCATCAGA+AGG 0.547608 1_3:-29439537 None:intergenic
ATAGCAGGGAACCCAACAGC+AGG 0.548068 1_3:-29441304 None:intergenic
TTCGTAATTTCTGGGTACCA+TGG 0.549342 1_3:+29439260 Msa0113440:exon
CTTTGTTTGCAATCGCGTGG+TGG 0.550397 1_3:+29440028 Msa0113440:CDS
TCATCAGAAGGAACCTCTTG+AGG 0.553244 1_3:-29439570 None:intergenic
TAAGAAGGGTGGCTCTAAAA+GGG 0.555483 1_3:+29441420 Msa0113440:CDS
GCAGACTCCTGCATGGTCAT+GGG 0.561161 1_3:-29440061 None:intergenic
TCAACCCTGCCTTCATCAGA+AGG 0.569263 1_3:-29439582 None:intergenic
GCAAACAAAGCCGCAAAGCA+TGG 0.573484 1_3:-29440015 None:intergenic
TGATGACGACGCAATTCCCT+CGG 0.574197 1_3:-29439436 None:intergenic
TGAGTTGGCACATGATGCCC+AGG 0.575121 1_3:-29441331 None:intergenic
TTGGGGTTCTAAATCGTCCA+TGG 0.576660 1_3:-29439277 None:intergenic
TCAATCTCTGAGAGAGGCGA+GGG 0.577892 1_3:-29439393 None:intergenic
TGTTGTTAGCCACAGTGGAA+GGG 0.582265 1_3:-29441360 None:intergenic
ATGATGATGAATGATTTGAA+TGG 0.584281 1_3:-29439233 None:intergenic
CTCAATCTCTGAGAGAGGCG+AGG 0.584965 1_3:-29439394 None:intergenic
GGTTCAGGAGGAACCTCTTG+AGG 0.589392 1_3:-29439615 None:intergenic
GTTACCTCCAAGAGAAAACA+TGG 0.596124 1_3:+29441385 Msa0113440:CDS
GGTTCCTTCTGATGAAGGCA+GGG 0.598695 1_3:+29439578 Msa0113440:CDS
CTGCTGTGAAGTCAAGAGAG+AGG 0.599823 1_3:+29439894 Msa0113440:CDS
TGATCCAGTAACTCCTCAAG+AGG 0.602779 1_3:+29439557 Msa0113440:CDS
GTGGAAGGGGTAGACTGAGT+TGG 0.606292 1_3:-29441346 None:intergenic
CGGCTTTGTTTGCAATCGCG+TGG 0.608761 1_3:+29440025 Msa0113440:CDS
GTGTCTGCGACGGAAACAGA+AGG 0.621465 1_3:-29439369 None:intergenic
CTTCTCATGTCTGATGCCGA+GGG 0.622387 1_3:+29439420 Msa0113440:CDS
AACACTGTTGTTAGCCACAG+TGG 0.628137 1_3:-29441365 None:intergenic
AAAATCTTGAGACGAAGAGT+AGG 0.630085 1_3:+29439933 Msa0113440:CDS
AGGCGAGGGAGTGTCTGCGA+CGG 0.634385 1_3:-29439379 None:intergenic
TGATCCAGTAACTCCTCAAG+AGG 0.636811 1_3:+29439602 Msa0113440:CDS
AGATTCTCAATCTCTGAGAG+AGG 0.640144 1_3:-29439399 None:intergenic
GGAACAAATAGACATCTGAA+AGG 0.644643 1_3:-29441567 None:intergenic
AGAGATTCGAAATCGATTTG+GGG 0.646761 1_3:-29439294 None:intergenic
GATTTGAATGGAATTAGTGA+AGG 0.655321 1_3:-29439221 None:intergenic
TTTCAGGCAGATGAGGAACA+GGG 0.660559 1_3:+29439868 Msa0113440:intron
GGAACACAAAGGTCTAAGAA+GGG 0.664648 1_3:+29441406 Msa0113440:CDS
GTGAGAGCGGTTATGAACAG+TGG 0.673673 1_3:-29439170 None:intergenic
TAGCAGGGAACCCAACAGCA+GGG 0.674308 1_3:-29441303 None:intergenic
ACACAAAGGTCTAAGAAGGG+TGG 0.678689 1_3:+29441409 Msa0113440:CDS
GAGCACAGCAGACTCCTGCA+TGG 0.688063 1_3:-29440068 None:intergenic
GATGCCCAGGAACCATAGCA+GGG 0.695327 1_3:-29441318 None:intergenic
AGAGAAAACATGGAACACAA+AGG 0.721160 1_3:+29441395 Msa0113440:CDS
TCAGTCTACCCCTTCCACTG+TGG 0.742334 1_3:+29441351 Msa0113440:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAATAAATATAAAAA+AGG - chr1_3:29439115-29439134 None:intergenic 0.0%
!! TAAGCAAAAAAAAAATTGTT+AGG - chr1_3:29440268-29440287 None:intergenic 15.0%
!! ATAGTTTCAATTTCATATAT+TGG + chr1_3:29440323-29440342 Msa0113440:intron 15.0%
!!! TTACATATTTTTTTGTAACT+AGG - chr1_3:29441676-29441695 None:intergenic 15.0%
!!! TAATTTGTAATTTGTATTCG+AGG + chr1_3:29439779-29439798 Msa0113440:intron 20.0%
!!! TGTCATTATCATTTTATTCT+AGG + chr1_3:29440145-29440164 Msa0113440:intron 20.0%
!! ATACATAAACTCACTAATTA+TGG - chr1_3:29440816-29440835 None:intergenic 20.0%
!! TAAATGGAAAAAAATTGTGT+GGG + chr1_3:29440930-29440949 Msa0113440:intron 20.0%
!! TACATAAAACATAAACAACA+TGG + chr1_3:29441091-29441110 Msa0113440:intron 20.0%
!! ATTCATTTAACATGCATAAA+GGG - chr1_3:29441147-29441166 None:intergenic 20.0%
!! TATTCATTTAACATGCATAA+AGG - chr1_3:29441148-29441167 None:intergenic 20.0%
!!! ATGTTATAAAACTTTTGAAG+TGG + chr1_3:29441542-29441561 Msa0113440:exon 20.0%
!!! TGTTATAAAACTTTTGAAGT+GGG + chr1_3:29441543-29441562 Msa0113440:exon 20.0%
!!! TTCTTCCTATTTGAAATATA+TGG + chr1_3:29441764-29441783 Msa0113440:three_prime_UTR 20.0%
!! AACTTCCATATATTTCAAAT+AGG - chr1_3:29441772-29441791 None:intergenic 20.0%
! AAGAAAAAAAAAAGTGAGAG+CGG - chr1_3:29439186-29439205 None:intergenic 25.0%
!! ATGATGATGAATGATTTGAA+TGG - chr1_3:29439236-29439255 None:intergenic 25.0%
!!! CTAATTTGCTTTTTTGAAAC+GGG - chr1_3:29439639-29439658 None:intergenic 25.0%
!!! CCTAATTTGCTTTTTTGAAA+CGG - chr1_3:29439640-29439659 None:intergenic 25.0%
!! CTAAAAGTATAACCATATGA+AGG + chr1_3:29440184-29440203 Msa0113440:intron 25.0%
! TGTTTCATTATTGCATTCTT+TGG + chr1_3:29440234-29440253 Msa0113440:intron 25.0%
!! TTTTTGCTTACTTTCTTGAA+TGG + chr1_3:29440278-29440297 Msa0113440:intron 25.0%
! AACTATATAGCATTACCAAA+AGG - chr1_3:29440309-29440328 None:intergenic 25.0%
! AGAGATTAAGGTTACAATTT+GGG - chr1_3:29440363-29440382 None:intergenic 25.0%
! TAGAGATTAAGGTTACAATT+TGG - chr1_3:29440364-29440383 None:intergenic 25.0%
!!! ACGTCTTTAGGTTTTTTATT+GGG + chr1_3:29440449-29440468 Msa0113440:intron 25.0%
! TTTAACAGTGTTATGAATAG+TGG + chr1_3:29440511-29440530 Msa0113440:intron 25.0%
!! AAACAGAAAACCTTAGATTT+TGG + chr1_3:29440685-29440704 Msa0113440:intron 25.0%
! CTAAATGGAAAAAAATTGTG+TGG + chr1_3:29440929-29440948 Msa0113440:intron 25.0%
!!! TCAGAGATATTTTTACTGTT+TGG - chr1_3:29441446-29441465 None:intergenic 25.0%
!!! GTTATAAAACTTTTGAAGTG+GGG + chr1_3:29441544-29441563 Msa0113440:exon 25.0%
! AATATGTAAACCAAACATGT+TGG + chr1_3:29441687-29441706 Msa0113440:three_prime_UTR 25.0%
! ATATGTAAACCAAACATGTT+GGG + chr1_3:29441688-29441707 Msa0113440:three_prime_UTR 25.0%
TTGTGTTGTCTTAGTGATTA+AGG - chr1_3:29439144-29439163 None:intergenic 30.0%
GATTTGAATGGAATTAGTGA+AGG - chr1_3:29439224-29439243 None:intergenic 30.0%
ATCATCTTCTTCGTAATTTC+TGG + chr1_3:29439251-29439270 Msa0113440:five_prime_UTR 30.0%
TCATCTTCTTCGTAATTTCT+GGG + chr1_3:29439252-29439271 Msa0113440:five_prime_UTR 30.0%
TTCGAGAAACTTATGATAGT+CGG - chr1_3:29439469-29439488 None:intergenic 30.0%
CCGTTTCAAAAAAGCAAATT+AGG + chr1_3:29439637-29439656 Msa0113440:CDS 30.0%
!!! TTTTTTTTTTTCCAACCTGC+TGG + chr1_3:29439754-29439773 Msa0113440:intron 30.0%
AAATTACAAATTACTCCAGC+AGG - chr1_3:29439772-29439791 None:intergenic 30.0%
!!! TGTTTTGGTATCTGATTTTC+AGG + chr1_3:29439852-29439871 Msa0113440:intron 30.0%
TATTGCATTCTTTGGTTGTT+TGG + chr1_3:29440242-29440261 Msa0113440:intron 30.0%
ACAGTAGTCATTAGAGATTA+AGG - chr1_3:29440375-29440394 None:intergenic 30.0%
ATAACAGCAAGAAGAAAACA+TGG - chr1_3:29440411-29440430 None:intergenic 30.0%
!!! GACGTCTTTAGGTTTTTTAT+TGG + chr1_3:29440448-29440467 Msa0113440:intron 30.0%
!! TCTAAGGTTTTCTGTTTCAT+GGG - chr1_3:29440682-29440701 None:intergenic 30.0%
!! ATCTAAGGTTTTCTGTTTCA+TGG - chr1_3:29440683-29440702 None:intergenic 30.0%
CTAAGATACTCCAAAATCTA+AGG - chr1_3:29440698-29440717 None:intergenic 30.0%
!!! CTTAGATTTTGGAGTATCTT+AGG + chr1_3:29440696-29440715 Msa0113440:intron 30.0%
ATAATATTTCACCTTCTCAC+TGG + chr1_3:29440783-29440802 Msa0113440:intron 30.0%
TAGTGAGTTTATGTATGTAC+TGG + chr1_3:29440820-29440839 Msa0113440:intron 30.0%
!! TTTAGGTTTTTGCGTCTAAA+TGG + chr1_3:29440914-29440933 Msa0113440:intron 30.0%
AAAAAAAAAAAGGCTACAGG+AGG - chr1_3:29441016-29441035 None:intergenic 30.0%
!!! TCCTGTAGCCTTTTTTTTTT+TGG + chr1_3:29441015-29441034 Msa0113440:intron 30.0%
ACCAAAAAAAAAAAGGCTAC+AGG - chr1_3:29441019-29441038 None:intergenic 30.0%
CCTTTGGACCAAAAAAAAAA+AGG - chr1_3:29441026-29441045 None:intergenic 30.0%
!!! CCTTTTTTTTTTTGGTCCAA+AGG + chr1_3:29441023-29441042 Msa0113440:intron 30.0%
TTTGCATAAGTACACAAATC+TGG - chr1_3:29441070-29441089 None:intergenic 30.0%
!!! TCTACTTTTTCTCTGAGTTT+TGG - chr1_3:29441122-29441141 None:intergenic 30.0%
! GTTTTATGAAGTTGCTCTAA+TGG + chr1_3:29441219-29441238 Msa0113440:intron 30.0%
TCTTTACAAAAGATTGAGTC+AGG - chr1_3:29441475-29441494 None:intergenic 30.0%
AGAGAAAATGCAAAGAAAGA+AGG - chr1_3:29441591-29441610 None:intergenic 30.0%
!! AGAGATTCGAAATCGATTTG+GGG - chr1_3:29439297-29439316 None:intergenic 35.0%
! GAGAGATTCGAAATCGATTT+GGG - chr1_3:29439298-29439317 None:intergenic 35.0%
AGAGAGATTCGAAATCGATT+TGG - chr1_3:29439299-29439318 None:intergenic 35.0%
!!! GGTTCTAATGAGAAGTGTTT+TGG + chr1_3:29439837-29439856 Msa0113440:intron 35.0%
AAAATCTTGAGACGAAGAGT+AGG + chr1_3:29439933-29439952 Msa0113440:CDS 35.0%
!! ACGAAGAGTAGGTATTTTGA+AGG + chr1_3:29439944-29439963 Msa0113440:CDS 35.0%
!! CGAAGAGTAGGTATTTTGAA+GGG + chr1_3:29439945-29439964 Msa0113440:CDS 35.0%
!! GAAGAGTAGGTATTTTGAAG+GGG + chr1_3:29439946-29439965 Msa0113440:CDS 35.0%
GAAACAAAATCATTCGTGCA+AGG - chr1_3:29440220-29440239 None:intergenic 35.0%
TGCTTACTTTCTTGAATGGA+TGG + chr1_3:29440282-29440301 Msa0113440:intron 35.0%
CCTAAAGACGTCTTTAACAT+CGG - chr1_3:29440440-29440459 None:intergenic 35.0%
TGTTATGAATAGTGGTGTAG+CGG + chr1_3:29440519-29440538 Msa0113440:intron 35.0%
AAAAACACCATGACAGTGTT+CGG + chr1_3:29440734-29440753 Msa0113440:intron 35.0%
TGACCTCAAAAACACTATAG+CGG - chr1_3:29440851-29440870 None:intergenic 35.0%
AGCAACTATGCTATGTTATC+TGG + chr1_3:29440870-29440889 Msa0113440:intron 35.0%
GCAACTATGCTATGTTATCT+GGG + chr1_3:29440871-29440890 Msa0113440:intron 35.0%
TAACAATCACAACCACTTGT+TGG + chr1_3:29440968-29440987 Msa0113440:intron 35.0%
CAAATCTGGATTACAGCAAA+AGG - chr1_3:29441056-29441075 None:intergenic 35.0%
! TTGTGTTCCATGTTTTCTCT+TGG - chr1_3:29441395-29441414 None:intergenic 35.0%
AGAGAAAACATGGAACACAA+AGG + chr1_3:29441395-29441414 Msa0113440:CDS 35.0%
GGAACAAATAGACATCTGAA+AGG - chr1_3:29441570-29441589 None:intergenic 35.0%
! TTCGTAATTTCTGGGTACCA+TGG + chr1_3:29439260-29439279 Msa0113440:exon 40.0%
CTCATCCTCCTGCAAAAAAT+CGG - chr1_3:29439340-29439359 None:intergenic 40.0%
! AGATTCTCAATCTCTGAGAG+AGG - chr1_3:29439402-29439421 None:intergenic 40.0%
AACCTTGATCATCCGATTGA+GGG - chr1_3:29439512-29439531 None:intergenic 40.0%
AAACCTTGATCATCCGATTG+AGG - chr1_3:29439513-29439532 None:intergenic 40.0%
!!! TTTTTTGAAACGGGTTCAGG+AGG - chr1_3:29439630-29439649 None:intergenic 40.0%
!!! TGCTTTTTTGAAACGGGTTC+AGG - chr1_3:29439633-29439652 None:intergenic 40.0%
TACAAATTACTCCAGCAGGT+TGG - chr1_3:29439768-29439787 None:intergenic 40.0%
TTCGAGGTTACCGAATTTGA+TGG + chr1_3:29439795-29439814 Msa0113440:intron 40.0%
TCGAGGTTACCGAATTTGAT+GGG + chr1_3:29439796-29439815 Msa0113440:intron 40.0%
CCATAAGACCCCATCAAATT+CGG - chr1_3:29439808-29439827 None:intergenic 40.0%
! CGAATTTGATGGGGTCTTAT+GGG + chr1_3:29439806-29439825 Msa0113440:intron 40.0%
!! ATCTGATTTTCAGGCAGATG+AGG + chr1_3:29439861-29439880 Msa0113440:intron 40.0%
GGTCACTGTAATCCTTCATA+TGG - chr1_3:29440199-29440218 None:intergenic 40.0%
! TCTTGAATGGATGGACCTTT+TGG + chr1_3:29440291-29440310 Msa0113440:intron 40.0%
CCGATGTTAAAGACGTCTTT+AGG + chr1_3:29440437-29440456 Msa0113440:intron 40.0%
ATTTCAGATCTCATCTCGTG+CGG - chr1_3:29440629-29440648 None:intergenic 40.0%
AAAACACCATGACAGTGTTC+GGG + chr1_3:29440735-29440754 Msa0113440:intron 40.0%
!! ATCCCGCTATAGTGTTTTTG+AGG + chr1_3:29440845-29440864 Msa0113440:intron 40.0%
GACCTCAAAAACACTATAGC+GGG - chr1_3:29440850-29440869 None:intergenic 40.0%
! GATACATACCACTGCTGTTT+AGG + chr1_3:29440897-29440916 Msa0113440:intron 40.0%
ATCTGGATTACAGCAAAAGG+AGG - chr1_3:29441053-29441072 None:intergenic 40.0%
TCTTCGAATGATGGCAACAT+TGG - chr1_3:29441195-29441214 None:intergenic 40.0%
AAAACCCACTCTTCGAATGA+TGG - chr1_3:29441204-29441223 None:intergenic 40.0%
GTTACCTCCAAGAGAAAACA+TGG + chr1_3:29441385-29441404 Msa0113440:CDS 40.0%
! TGTTCCATGTTTTCTCTTGG+AGG - chr1_3:29441392-29441411 None:intergenic 40.0%
TGGAACACAAAGGTCTAAGA+AGG + chr1_3:29441405-29441424 Msa0113440:CDS 40.0%
GGAACACAAAGGTCTAAGAA+GGG + chr1_3:29441406-29441425 Msa0113440:CDS 40.0%
!! TAAGAAGGGTGGCTCTAAAA+GGG + chr1_3:29441420-29441439 Msa0113440:CDS 40.0%
TCATCTTCGTTCTTGAAGCT+TGG - chr1_3:29441508-29441527 None:intergenic 40.0%
ACCAAAAAGTGAGAGAGAGA+GGG - chr1_3:29441624-29441643 None:intergenic 40.0%
AACCAAAAAGTGAGAGAGAG+AGG - chr1_3:29441625-29441644 None:intergenic 40.0%
!! CAAAAGCATCCCAACATGTT+TGG - chr1_3:29441700-29441719 None:intergenic 40.0%
! CAAACATGTTGGGATGCTTT+TGG + chr1_3:29441698-29441717 Msa0113440:three_prime_UTR 40.0%
!! TTGGGGTTCTAAATCGTCCA+TGG - chr1_3:29439280-29439299 None:intergenic 45.0%
!!! CGATTTTTTGCAGGAGGATG+AGG + chr1_3:29439338-29439357 Msa0113440:CDS 45.0%
GATCATCCGATTGAGGGATT+GGG - chr1_3:29439506-29439525 None:intergenic 45.0%
TGATCATCCGATTGAGGGAT+TGG - chr1_3:29439507-29439526 None:intergenic 45.0%
ATCCCTCAATCGGATGATCA+AGG + chr1_3:29439507-29439526 Msa0113440:CDS 45.0%
! TCAACTCTGGCTTCATCAGA+AGG - chr1_3:29439540-29439559 None:intergenic 45.0%
AGGAGTTACTGGATCAACTC+TGG - chr1_3:29439553-29439572 None:intergenic 45.0%
TGATCCAGTAACTCCTCAAG+AGG + chr1_3:29439557-29439576 Msa0113440:CDS 45.0%
TCATCAGAAGGAACCTCTTG+AGG - chr1_3:29439573-29439592 None:intergenic 45.0%
CAAGAGGTTCCTTCTGATGA+AGG + chr1_3:29439573-29439592 Msa0113440:CDS 45.0%
TGATCCAGTAACTCCTCAAG+AGG + chr1_3:29439557-29439576 Msa0113440:CDS 45.0%
CGAGGTTACCGAATTTGATG+GGG + chr1_3:29439797-29439816 Msa0113440:intron 45.0%
CCGAATTTGATGGGGTCTTA+TGG + chr1_3:29439805-29439824 Msa0113440:intron 45.0%
! GGGGTCTTATGGGATGATTA+TGG + chr1_3:29439816-29439835 Msa0113440:intron 45.0%
!! TTTTCAGGCAGATGAGGAAC+AGG + chr1_3:29439867-29439886 Msa0113440:intron 45.0%
TTTCAGGCAGATGAGGAACA+GGG + chr1_3:29439868-29439887 Msa0113440:intron 45.0%
ATGCTGAGAACCATGCTTTG+CGG + chr1_3:29440005-29440024 Msa0113440:CDS 45.0%
! AGCTTGTTTGTAGCAGCCAA+GGG - chr1_3:29440550-29440569 None:intergenic 45.0%
GGCCAATATAGCTCATGTTG+CGG + chr1_3:29440592-29440611 Msa0113440:intron 45.0%
CTTCTCATGCTGTCTCATTC+AGG + chr1_3:29440758-29440777 Msa0113440:intron 45.0%
CGCAAAAACCTAAACAGCAG+TGG - chr1_3:29440908-29440927 None:intergenic 45.0%
CTCTGACAAAGACCAACAAG+TGG - chr1_3:29440983-29441002 None:intergenic 45.0%
! AGCAAAAGGAGGTACACCTT+TGG - chr1_3:29441042-29441061 None:intergenic 45.0%
TGTTGCCATCATTCGAAGAG+TGG + chr1_3:29441196-29441215 Msa0113440:intron 45.0%
GTTGCCATCATTCGAAGAGT+GGG + chr1_3:29441197-29441216 Msa0113440:intron 45.0%
! TTTTGCAGAATCCCTGCTGT+TGG + chr1_3:29441292-29441311 Msa0113440:intron 45.0%
TTTGCAGAATCCCTGCTGTT+GGG + chr1_3:29441293-29441312 Msa0113440:intron 45.0%
TGTTGTTAGCCACAGTGGAA+GGG - chr1_3:29441363-29441382 None:intergenic 45.0%
AACACTGTTGTTAGCCACAG+TGG - chr1_3:29441368-29441387 None:intergenic 45.0%
ACACAAAGGTCTAAGAAGGG+TGG + chr1_3:29441409-29441428 Msa0113440:CDS 45.0%
!! CTAAGAAGGGTGGCTCTAAA+AGG + chr1_3:29441419-29441438 Msa0113440:CDS 45.0%
!! TCCCTCTCTCTCTCACTTTT+TGG + chr1_3:29441620-29441639 Msa0113440:three_prime_UTR 45.0%
GTGAGAGCGGTTATGAACAG+TGG - chr1_3:29439173-29439192 None:intergenic 50.0%
!!! CGAAGTCGCCGATTTTTTGC+AGG + chr1_3:29439329-29439348 Msa0113440:CDS 50.0%
!!! AGTCGCCGATTTTTTGCAGG+AGG + chr1_3:29439332-29439351 Msa0113440:CDS 50.0%
! TCAATCTCTGAGAGAGGCGA+GGG - chr1_3:29439396-29439415 None:intergenic 50.0%
TCTTCTCATGTCTGATGCCG+AGG + chr1_3:29439419-29439438 Msa0113440:CDS 50.0%
CTTCTCATGTCTGATGCCGA+GGG + chr1_3:29439420-29439439 Msa0113440:CDS 50.0%
! TGATGACGACGCAATTCCCT+CGG - chr1_3:29439439-29439458 None:intergenic 50.0%
GGGAATTGCGTCGTCATCAT+CGG + chr1_3:29439440-29439459 Msa0113440:CDS 50.0%
GGAACCTCTTGAGGAGTTAC+TGG - chr1_3:29439564-29439583 None:intergenic 50.0%
AGGTTCCTTCTGATGAAGGC+AGG + chr1_3:29439577-29439596 Msa0113440:CDS 50.0%
GGTTCCTTCTGATGAAGGCA+GGG + chr1_3:29439578-29439597 Msa0113440:CDS 50.0%
TCAACCCTGCCTTCATCAGA+AGG - chr1_3:29439585-29439604 None:intergenic 50.0%
GGAACCTCTTGAGGAGTTAC+TGG - chr1_3:29439564-29439583 None:intergenic 50.0%
CTGCTGTGAAGTCAAGAGAG+AGG + chr1_3:29439894-29439913 Msa0113440:CDS 50.0%
TGAAGGGGAATGCAGAAGAC+TGG + chr1_3:29439961-29439980 Msa0113440:CDS 50.0%
GCAAACAAAGCCGCAAAGCA+TGG - chr1_3:29440018-29440037 None:intergenic 50.0%
! CTTTGTTTGCAATCGCGTGG+TGG + chr1_3:29440028-29440047 Msa0113440:CDS 50.0%
GAGTCTGCTGTGCTCTTGTT+GGG + chr1_3:29440076-29440095 Msa0113440:CDS 50.0%
TGCTGTGCTCTTGTTGGGTA+AGG + chr1_3:29440081-29440100 Msa0113440:intron 50.0%
! TGAATAGTGGTGTAGCGGAG+TGG + chr1_3:29440524-29440543 Msa0113440:intron 50.0%
! CAGCTTGTTTGTAGCAGCCA+AGG - chr1_3:29440551-29440570 None:intergenic 50.0%
GGCCGCAACATGAGCTATAT+TGG - chr1_3:29440597-29440616 None:intergenic 50.0%
ATGGTGACTGACCAGTGAGA+AGG - chr1_3:29440797-29440816 None:intergenic 50.0%
GTTGTTAGCCACAGTGGAAG+GGG - chr1_3:29441362-29441381 None:intergenic 50.0%
CTGTTGTTAGCCACAGTGGA+AGG - chr1_3:29441364-29441383 None:intergenic 50.0%
GTGTCTGCGACGGAAACAGA+AGG - chr1_3:29439372-29439391 None:intergenic 55.0%
! CTCAATCTCTGAGAGAGGCG+AGG - chr1_3:29439397-29439416 None:intergenic 55.0%
CCTCGACCCAATCCCTCAAT+CGG + chr1_3:29439497-29439516 Msa0113440:CDS 55.0%
GGTTCAGGAGGAACCTCTTG+AGG - chr1_3:29439618-29439637 None:intergenic 55.0%
!! CGGCTTTGTTTGCAATCGCG+TGG + chr1_3:29440025-29440044 Msa0113440:CDS 55.0%
TTGCAATCGCGTGGTGGCTT+TGG + chr1_3:29440034-29440053 Msa0113440:CDS 55.0%
GCAGACTCCTGCATGGTCAT+GGG - chr1_3:29440064-29440083 None:intergenic 55.0%
AGCAGACTCCTGCATGGTCA+TGG - chr1_3:29440065-29440084 None:intergenic 55.0%
GGAGTCTGCTGTGCTCTTGT+TGG + chr1_3:29440075-29440094 Msa0113440:CDS 55.0%
TAGTGCAGCTGCTGCTAGAG+CGG + chr1_3:29440571-29440590 Msa0113440:intron 55.0%
CATCTCGTGCGGCACTTTAG+AGG - chr1_3:29440618-29440637 None:intergenic 55.0%
GAGAAGCCCGAACACTGTCA+TGG - chr1_3:29440744-29440763 None:intergenic 55.0%
TAGCAGGGAACCCAACAGCA+GGG - chr1_3:29441306-29441325 None:intergenic 55.0%
ATAGCAGGGAACCCAACAGC+AGG - chr1_3:29441307-29441326 None:intergenic 55.0%
TGCTGTTGGGTTCCCTGCTA+TGG + chr1_3:29441306-29441325 Msa0113440:CDS 55.0%
GGTTCCCTGCTATGGTTCCT+GGG + chr1_3:29441314-29441333 Msa0113440:CDS 55.0%
GATGCCCAGGAACCATAGCA+GGG - chr1_3:29441321-29441340 None:intergenic 55.0%
TGATGCCCAGGAACCATAGC+AGG - chr1_3:29441322-29441341 None:intergenic 55.0%
! TGAGTTGGCACATGATGCCC+AGG - chr1_3:29441334-29441353 None:intergenic 55.0%
GTGGAAGGGGTAGACTGAGT+TGG - chr1_3:29441349-29441368 None:intergenic 55.0%
TCAGTCTACCCCTTCCACTG+TGG + chr1_3:29441351-29441370 Msa0113440:CDS 55.0%
CCGATTGAGGGATTGGGTCG+AGG - chr1_3:29439500-29439519 None:intergenic 60.0%
TGGTGCTCCCATGACCATGC+AGG + chr1_3:29440054-29440073 Msa0113440:CDS 60.0%
GAGCACAGCAGACTCCTGCA+TGG - chr1_3:29440071-29440090 None:intergenic 60.0%
GGGTTCCCTGCTATGGTTCC+TGG + chr1_3:29441313-29441332 Msa0113440:CDS 60.0%
!! AGGCGAGGGAGTGTCTGCGA+CGG - chr1_3:29439382-29439401 None:intergenic 65.0%
! TGGTGTAGCGGAGTGGCCCT+TGG + chr1_3:29440531-29440550 Msa0113440:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 29439109 29441833 29439109 ID=Msa0113440;Name=Msa0113440
chr1_3 mRNA 29439109 29441833 29439109 ID=Msa0113440-mRNA-1;Parent=Msa0113440;Name=Msa0113440-mRNA-1;_AED=0.01;_eAED=0.01;_QI=170|1|1|1|0.5|1|3|283|284
chr1_3 exon 29439109 29439658 29439109 ID=Msa0113440-mRNA-1:exon:9694;Parent=Msa0113440-mRNA-1
chr1_3 exon 29439874 29440097 29439874 ID=Msa0113440-mRNA-1:exon:9695;Parent=Msa0113440-mRNA-1
chr1_3 exon 29441300 29441833 29441300 ID=Msa0113440-mRNA-1:exon:9696;Parent=Msa0113440-mRNA-1
chr1_3 five_prime_UTR 29439109 29439278 29439109 ID=Msa0113440-mRNA-1:five_prime_utr;Parent=Msa0113440-mRNA-1
chr1_3 CDS 29439279 29439658 29439279 ID=Msa0113440-mRNA-1:cds;Parent=Msa0113440-mRNA-1
chr1_3 CDS 29439874 29440097 29439874 ID=Msa0113440-mRNA-1:cds;Parent=Msa0113440-mRNA-1
chr1_3 CDS 29441300 29441550 29441300 ID=Msa0113440-mRNA-1:cds;Parent=Msa0113440-mRNA-1
chr1_3 three_prime_UTR 29441551 29441833 29441551 ID=Msa0113440-mRNA-1:three_prime_utr;Parent=Msa0113440-mRNA-1
Gene Sequence

>Msa0113440

ATGGACGATTTAGAACCCCAAATCGATTTCGAATCTCTCTTCGCAGAATACGAAGTCGCCGATTTTTTGCAGGAGGATGAGGATATCAATCCTTCTGTTTCCGTCGCAGACACTCCCTCGCCTCTCTCAGAGATTGAGAATCTTCTCATGTCTGATGCCGAGGGAATTGCGTCGTCATCATCGGATTCCGACTATCATAAGTTTCTCGAAGATATTCTCCTCGACCCAATCCCTCAATCGGATGATCAAGGTTTCGTTCCTTCTGATGAAGCCAGAGTTGATCCAGTAACTCCTCAAGAGGTTCCTTCTGATGAAGGCAGGGTTGATCCAGTAACTCCTCAAGAGGTTCCTCCTGAACCCGTTTCAAAAAAGCAAATTAGGCAGATGAGGAACAGGGATGCTGCTGTGAAGTCAAGAGAGAGGAAGAAGATGTACGTGAAAAATCTTGAGACGAAGAGTAGGTATTTTGAAGGGGAATGCAGAAGACTGGAGCATTTGCTTCAGTGCTGCTATGCTGAGAACCATGCTTTGCGGCTTTGTTTGCAATCGCGTGGTGGCTTTGGTGCTCCCATGACCATGCAGGAGTCTGCTGTGCTCTTGTTGGAATCCCTGCTGTTGGGTTCCCTGCTATGGTTCCTGGGCATCATGTGCCAACTCAGTCTACCCCTTCCACTGTGGCTAACAACAGTGTTACCTCCAAGAGAAAACATGGAACACAAAGGTCTAAGAAGGGTGGCTCTAAAAGGGCCAAACAGTAAAAATATCTCTGATTATTTCCTGACTCAATCTTTTGTAAAGAGTAGAAGATGCCAAGCTTCAAGAACGAAGATGAAATTTGATTTTATCATGTTATAA
Protein sequence

>Msa0113440

MDDLEPQIDFESLFAEYEVADFLQEDEDINPSVSVADTPSPLSEIENLLMSDAEGIASSSSDSDYHKFLEDILLDPIPQSDDQGFVPSDEARVDPVTPQEVPSDEGRVDPVTPQEVPPEPVSKKQIRQMRNRDAAVKSRERKKMYVKNLETKSRYFEGECRRLEHLLQCCYAENHALRLCLQSRGGFGAPMTMQESAVLLLESLLLGSLLWFLGIMCQLSLPLPLWLTTVLPPRENMEHKGLRRVALKGPNSKNISDYFLTQSFVKSRRCQASRTKMKFDFIML*