Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0114420 | AES97386.1 | 84.211 | 76 | 12 | 0 | 1 | 76 | 34 | 109 | 4.00e-38 | 132 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0114420 | G7JX45 | 84.211 | 76 | 12 | 0 | 1 | 76 | 34 | 109 | 1.91e-38 | 132 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0114420 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 8 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCCGTTTCAAGTTCTTCTA+TGG | 0.193225 | 1_3:+31286069 | Msa0114420:CDS |
| AACGGATAGTGTCTCCAATC+TGG | 0.396291 | 1_3:-31286053 | None:intergenic |
| TATTCAGCGATTCATTGCTT+TGG | 0.421950 | 1_3:-31285984 | None:intergenic |
| AACAATAATGCATTCCAGAT+TGG | 0.451495 | 1_3:+31286039 | Msa0114420:CDS |
| GTCCGTTTGTTCATGTCTAT+AGG | 0.453841 | 1_3:+31286100 | Msa0114420:CDS |
| TCCCATAGAAGAACTTGAAA+CGG | 0.465685 | 1_3:-31286071 | None:intergenic |
| TCCGTTTCAAGTTCTTCTAT+GGG | 0.483891 | 1_3:+31286070 | Msa0114420:CDS |
| TTCCTATAGACATGAACAAA+CGG | 0.529107 | 1_3:-31286102 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATTTGTGATTACAATAA+AGG | - | chr1_3:31285788-31285807 | None:intergenic | 15.0% |
| !! | ACATAATTTAGTTAGAATAA+AGG | - | chr1_3:31285815-31285834 | None:intergenic | 15.0% |
| !!! | ATACATGTTTTACAAATTAT+AGG | + | chr1_3:31285855-31285874 | Msa0114420:intron | 15.0% |
| !! | AAAACATGTATTTGAGAATT+TGG | - | chr1_3:31285846-31285865 | None:intergenic | 20.0% |
| !! | CTGAATACTAAGTTAAAAAA+CGG | + | chr1_3:31286000-31286019 | Msa0114420:CDS | 20.0% |
| ! | TATAAGAAATCATTGAAGTC+TGG | - | chr1_3:31285889-31285908 | None:intergenic | 25.0% |
| ! | AGACTTCAATGATTTCTTAT+AGG | + | chr1_3:31285888-31285907 | Msa0114420:intron | 25.0% |
| ! | ATACTAAGTTAAAAAACGGT+TGG | + | chr1_3:31286004-31286023 | Msa0114420:CDS | 25.0% |
| ! | TACTAAGTTAAAAAACGGTT+GGG | + | chr1_3:31286005-31286024 | Msa0114420:CDS | 25.0% |
| ! | CAATAAAGGAGCACATAATT+TGG | - | chr1_3:31285774-31285793 | None:intergenic | 30.0% |
| ! | GATTTTCATTGCACAGAAAA+AGG | - | chr1_3:31285930-31285949 | None:intergenic | 30.0% |
| ! | GCTGTAACAAACTTTTCATT+CGG | - | chr1_3:31285952-31285971 | None:intergenic | 30.0% |
| AACAATAATGCATTCCAGAT+TGG | + | chr1_3:31286039-31286058 | Msa0114420:CDS | 30.0% | |
| TTCCTATAGACATGAACAAA+CGG | - | chr1_3:31286105-31286124 | None:intergenic | 30.0% | |
| AAGAAATCATTGAAGTCTGG+TGG | - | chr1_3:31285886-31285905 | None:intergenic | 35.0% | |
| TATTCAGCGATTCATTGCTT+TGG | - | chr1_3:31285987-31286006 | None:intergenic | 35.0% | |
| !! | ATCCGTTTCAAGTTCTTCTA+TGG | + | chr1_3:31286069-31286088 | Msa0114420:CDS | 35.0% |
| !! | TCCGTTTCAAGTTCTTCTAT+GGG | + | chr1_3:31286070-31286089 | Msa0114420:CDS | 35.0% |
| TCCCATAGAAGAACTTGAAA+CGG | - | chr1_3:31286074-31286093 | None:intergenic | 35.0% | |
| GTCCGTTTGTTCATGTCTAT+AGG | + | chr1_3:31286100-31286119 | Msa0114420:CDS | 40.0% | |
| AACGGATAGTGTCTCCAATC+TGG | - | chr1_3:31286056-31286075 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_3 | gene | 31285740 | 31286134 | 31285740 | ID=Msa0114420;Name=Msa0114420 |
| chr1_3 | mRNA | 31285740 | 31286134 | 31285740 | ID=Msa0114420-mRNA-1;Parent=Msa0114420;Name=Msa0114420-mRNA-1;_AED=0.50;_eAED=0.50;_QI=0|0|0|0.5|1|1|2|0|76 |
| chr1_3 | exon | 31285740 | 31285745 | 31285740 | ID=Msa0114420-mRNA-1:exon:10250;Parent=Msa0114420-mRNA-1 |
| chr1_3 | exon | 31285910 | 31286134 | 31285910 | ID=Msa0114420-mRNA-1:exon:10251;Parent=Msa0114420-mRNA-1 |
| chr1_3 | CDS | 31285740 | 31285745 | 31285740 | ID=Msa0114420-mRNA-1:cds;Parent=Msa0114420-mRNA-1 |
| chr1_3 | CDS | 31285910 | 31286134 | 31285910 | ID=Msa0114420-mRNA-1:cds;Parent=Msa0114420-mRNA-1 |
| Gene Sequence |
| Protein sequence |
