AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0117780

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0117780 MtrunA17_Chr1g0174911 86.707 331 34 2 1 329 1 323 0.0 566
Msa0117780 MtrunA17_Chr1g0168571 57.593 349 102 11 1 328 1 324 5.08e-116 338
Msa0117780 MtrunA17_Chr7g0274971 60.976 205 53 6 1 183 1 200 4.35e-76 233
Msa0117780 MtrunA17_Chr7g0214071 32.143 168 91 9 11 170 19 171 4.83e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 59 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAAGAATCCTTGGGATATT+TGG 0.040443 1_3:-39436758 Msa0117780:CDS
CCACCATTTGATTCGTTTCT+TGG 0.076491 1_3:+39436101 None:intergenic
TAACTCTTCATGAAACTTAT+TGG 0.168233 1_3:+39435141 None:intergenic
GTTTCATGAAGAGTTATTAT+TGG 0.203680 1_3:-39435134 Msa0117780:CDS
AAATATCCCAAGGATTCTTT+AGG 0.262491 1_3:+39436760 None:intergenic
CCAAGAGAGACAACCATTCT+TGG 0.271875 1_3:-39436005 Msa0117780:CDS
AACTCTTCATGAAACTTATT+GGG 0.303213 1_3:+39435142 None:intergenic
TCAAAAGTAGTTCGTTCTAT+TGG 0.321604 1_3:-39436648 Msa0117780:CDS
AAAGAATCCTTGGGATATTT+GGG 0.338135 1_3:-39436757 Msa0117780:CDS
TGGGATATTTGGGAAGAGTT+TGG 0.341365 1_3:-39436747 Msa0117780:CDS
AGTTGGGCAGAGATTTGTTA+TGG 0.350279 1_3:+39436817 None:intergenic
ATTCTTGAATACGATTTGTT+TGG 0.353129 1_3:-39436786 Msa0117780:CDS
CAGGTTGAACTATTTGATTT+TGG 0.356930 1_3:+39435338 None:intergenic
GTAGTTCGTTCTATTGGAAT+TGG 0.365191 1_3:-39436642 Msa0117780:CDS
ATCTTTGATAAGAAATCCTT+TGG 0.365896 1_3:-39436472 Msa0117780:intron
TCTCAATTCTTCTTCAAGTA+AGG 0.366602 1_3:-39436895 Msa0117780:CDS
AATATCCCAAGGATTCTTTA+GGG 0.371252 1_3:+39436761 None:intergenic
TGGACACAATGGCACAAATA+TGG 0.371907 1_3:-39436081 Msa0117780:CDS
TAGTGGTAGATTTCTTCTCT+AGG 0.391403 1_3:+39436673 None:intergenic
TGTAGGACTTACAAATTTGT+TGG 0.396611 1_3:+39435971 None:intergenic
TTAAGGTAGTGAAGAAGTAT+AGG 0.409073 1_3:+39436697 None:intergenic
CCAAGAATGGTTGTCTCTCT+TGG 0.410667 1_3:+39436005 None:intergenic
AGATTTCTTCTCTAGGGTTA+AGG 0.417289 1_3:+39436680 None:intergenic
GAAAGAAAGACCATTGTTCC+TGG 0.417462 1_3:-39436304 Msa0117780:CDS
TTGGTACCCTAAAGAATCCT+TGG 0.424102 1_3:-39436767 Msa0117780:CDS
TTCAAGAATGGTGATATAGT+TGG 0.425134 1_3:+39436800 None:intergenic
TCATCATCATCTTCATTGTC+AGG 0.425138 1_3:+39435319 None:intergenic
AATCAAGTAACTTACAGATA+TGG 0.426262 1_3:+39436267 None:intergenic
TCTGCATAGAACATAATTGT+TGG 0.465415 1_3:+39436357 None:intergenic
TCAAGAATGGTGATATAGTT+GGG 0.477899 1_3:+39436801 None:intergenic
TGGTACCCTAAAGAATCCTT+GGG 0.489046 1_3:-39436766 Msa0117780:CDS
AGTGGTAGATTTCTTCTCTA+GGG 0.491270 1_3:+39436674 None:intergenic
ATGCTTGTTAGAAGAGGAAG+AGG 0.495031 1_3:-39435176 Msa0117780:CDS
GAACCAAGAAACGAATCAAA+TGG 0.503929 1_3:-39436104 Msa0117780:CDS
GTTCTATTGGAATTGGTGCA+TGG 0.508402 1_3:-39436635 Msa0117780:CDS
GAGCTGATAGAGATTAATGA+AGG 0.519458 1_3:-39435199 Msa0117780:CDS
AAACAAATCGTATTCAAGAA+TGG 0.519788 1_3:+39436788 None:intergenic
TGAAGGATGCTTGTTAGAAG+AGG 0.520085 1_3:-39435182 Msa0117780:CDS
GGCTGCTTCGAGTCCAAGAA+TGG 0.535306 1_3:+39435992 None:intergenic
ATGGTGGTTTCTGCACGTCC+AGG 0.545124 1_3:+39436286 None:intergenic
TTCTATTGGAATTGGTGCAT+GGG 0.546914 1_3:-39436634 Msa0117780:CDS
GCAAGATCCATGCACCATCA+TGG 0.566204 1_3:+39436514 None:intergenic
TTCTTATCAAAGATTTGTCG+AGG 0.569506 1_3:+39436481 None:intergenic
ATGATGATGCTGGTATGTCT+TGG 0.577942 1_3:-39435240 Msa0117780:CDS
AACTCTTCCCAAATATCCCA+AGG 0.585203 1_3:+39436750 None:intergenic
CAAGTAACTTACAGATATGG+TGG 0.592493 1_3:+39436270 None:intergenic
TTCTGCACGTCCAGGAACAA+TGG 0.595867 1_3:+39436294 None:intergenic
GAATCAAATGGTGGACACAA+TGG 0.599759 1_3:-39436092 Msa0117780:CDS
GACAATGAAGATGATGATGA+TGG 0.618553 1_3:-39435316 Msa0117780:CDS
AGAAAGAATATTCATGCACA+AGG 0.627598 1_3:-39436331 Msa0117780:CDS
ATTGGAATTGGTGCATGGGA+AGG 0.630255 1_3:-39436630 Msa0117780:CDS
GCACTTACCATGATGGTGCA+TGG 0.633065 1_3:-39436521 Msa0117780:CDS
GACGAAGAAGATGATGATGC+TGG 0.640357 1_3:-39435250 Msa0117780:CDS
AATAGTGGCACTTACCATGA+TGG 0.645072 1_3:-39436528 Msa0117780:CDS
AAATGATGGTGAAAGCATTG+TGG 0.661701 1_3:-39435272 Msa0117780:CDS
TGGGAAGGTGAAGAAGACAC+TGG 0.669846 1_3:-39436615 Msa0117780:CDS
CCAAGAAACGAATCAAATGG+TGG 0.672770 1_3:-39436101 Msa0117780:CDS
TGATGATGGTGAATGCATTG+TGG 0.673578 1_3:-39435302 Msa0117780:CDS
ATTGTGGACAAAGAAAATGA+TGG 0.682219 1_3:-39435286 Msa0117780:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTATCTTTTTTTTTTTTGT+AGG - chr1_3:39435634-39435653 Msa0117780:intron 15.0%
!! AAAAAGATGTTAGAAATATT+TGG - chr1_3:39435986-39436005 Msa0117780:CDS 15.0%
!!! CATTTTTAAGTATTCAATAA+TGG - chr1_3:39436243-39436262 Msa0117780:intron 15.0%
!!! TCCTATTTTTATCTTATTTT+TGG - chr1_3:39436270-39436289 Msa0117780:intron 15.0%
!!! CCTATTTTTATCTTATTTTT+GGG - chr1_3:39436271-39436290 Msa0117780:intron 15.0%
!!! TCAAATGTTTTTCATAAAAT+AGG + chr1_3:39436455-39436474 None:intergenic 15.0%
!!! TGTATCTTTTTTTTTTTTGT+AGG - chr1_3:39435634-39435653 Msa0117780:intron 15.0%
!! AAAAAGATGTTAGAAATATT+TGG - chr1_3:39435986-39436005 Msa0117780:CDS 15.0%
!!! CATTTTTAAGTATTCAATAA+TGG - chr1_3:39436243-39436262 Msa0117780:intron 15.0%
!!! TCCTATTTTTATCTTATTTT+TGG - chr1_3:39436270-39436289 Msa0117780:intron 15.0%
!!! CCTATTTTTATCTTATTTTT+GGG - chr1_3:39436271-39436290 Msa0117780:intron 15.0%
!!! TCAAATGTTTTTCATAAAAT+AGG + chr1_3:39436455-39436474 None:intergenic 15.0%
!! CTTGATTCAAGATTTAATAA+TGG - chr1_3:39435780-39435799 Msa0117780:intron 20.0%
!! AGAAAAACAACAATAAATGT+AGG + chr1_3:39436063-39436082 None:intergenic 20.0%
!! AACATATTAACACAATTATC+TGG - chr1_3:39436151-39436170 Msa0117780:intron 20.0%
!! CCCAAAAATAAGATAAAAAT+AGG + chr1_3:39436274-39436293 None:intergenic 20.0%
!!! TGTTTTCATCTGAATAAAAA+TGG + chr1_3:39436425-39436444 None:intergenic 20.0%
!! AATTAGAATACAAAATGAGT+TGG + chr1_3:39436597-39436616 None:intergenic 20.0%
!! CTTGATTCAAGATTTAATAA+TGG - chr1_3:39435780-39435799 Msa0117780:intron 20.0%
!! AGAAAAACAACAATAAATGT+AGG + chr1_3:39436063-39436082 None:intergenic 20.0%
!! AACATATTAACACAATTATC+TGG - chr1_3:39436151-39436170 Msa0117780:intron 20.0%
!! CCCAAAAATAAGATAAAAAT+AGG + chr1_3:39436274-39436293 None:intergenic 20.0%
!!! TGTTTTCATCTGAATAAAAA+TGG + chr1_3:39436425-39436444 None:intergenic 20.0%
!! AATTAGAATACAAAATGAGT+TGG + chr1_3:39436597-39436616 None:intergenic 20.0%
! AAACAAATCGTATTCAAGAA+TGG + chr1_3:39435229-39435248 None:intergenic 25.0%
!! ATTCTTGAATACGATTTGTT+TGG - chr1_3:39435228-39435247 Msa0117780:CDS 25.0%
! ATCTTTGATAAGAAATCCTT+TGG - chr1_3:39435542-39435561 Msa0117780:intron 25.0%
! AATCAAGTAACTTACAGATA+TGG + chr1_3:39435750-39435769 None:intergenic 25.0%
! ATGTTAAGTAGAAAAACAAC+CGG + chr1_3:39435820-39435839 None:intergenic 25.0%
!! CATTCTTGATTTGATATGTA+GGG - chr1_3:39435850-39435869 Msa0117780:intron 25.0%
! AATGATAGTAACATACTAAC+TGG + chr1_3:39436227-39436246 None:intergenic 25.0%
! TGAACATGAAAAAAAGGTTA+AGG + chr1_3:39436480-39436499 None:intergenic 25.0%
!!! ACCTTTTTTTCATGTTCAAT+AGG - chr1_3:39436482-39436501 Msa0117780:CDS 25.0%
! ACCTATTGAACATGAAAAAA+AGG + chr1_3:39436486-39436505 None:intergenic 25.0%
! AACTCTTCATGAAACTTATT+GGG + chr1_3:39436875-39436894 None:intergenic 25.0%
! TAACTCTTCATGAAACTTAT+TGG + chr1_3:39436876-39436895 None:intergenic 25.0%
! GTTTCATGAAGAGTTATTAT+TGG - chr1_3:39436880-39436899 Msa0117780:CDS 25.0%
! AAACAAATCGTATTCAAGAA+TGG + chr1_3:39435229-39435248 None:intergenic 25.0%
!! ATTCTTGAATACGATTTGTT+TGG - chr1_3:39435228-39435247 Msa0117780:CDS 25.0%
! ATCTTTGATAAGAAATCCTT+TGG - chr1_3:39435542-39435561 Msa0117780:intron 25.0%
! AATCAAGTAACTTACAGATA+TGG + chr1_3:39435750-39435769 None:intergenic 25.0%
! ATGTTAAGTAGAAAAACAAC+CGG + chr1_3:39435820-39435839 None:intergenic 25.0%
!! CATTCTTGATTTGATATGTA+GGG - chr1_3:39435850-39435869 Msa0117780:intron 25.0%
! AATGATAGTAACATACTAAC+TGG + chr1_3:39436227-39436246 None:intergenic 25.0%
! TGAACATGAAAAAAAGGTTA+AGG + chr1_3:39436480-39436499 None:intergenic 25.0%
!!! ACCTTTTTTTCATGTTCAAT+AGG - chr1_3:39436482-39436501 Msa0117780:CDS 25.0%
! ACCTATTGAACATGAAAAAA+AGG + chr1_3:39436486-39436505 None:intergenic 25.0%
! AACTCTTCATGAAACTTATT+GGG + chr1_3:39436875-39436894 None:intergenic 25.0%
! TAACTCTTCATGAAACTTAT+TGG + chr1_3:39436876-39436895 None:intergenic 25.0%
! GTTTCATGAAGAGTTATTAT+TGG - chr1_3:39436880-39436899 Msa0117780:CDS 25.0%
TCTCAATTCTTCTTCAAGTA+AGG - chr1_3:39435119-39435138 Msa0117780:CDS 30.0%
!! TGTTATGGATTTTGTTGTAG+AGG + chr1_3:39435185-39435204 None:intergenic 30.0%
TCAAGAATGGTGATATAGTT+GGG + chr1_3:39435216-39435235 None:intergenic 30.0%
TTCAAGAATGGTGATATAGT+TGG + chr1_3:39435217-39435236 None:intergenic 30.0%
AATATCCCAAGGATTCTTTA+GGG + chr1_3:39435256-39435275 None:intergenic 30.0%
AAATATCCCAAGGATTCTTT+AGG + chr1_3:39435257-39435276 None:intergenic 30.0%
TAAAGAATCCTTGGGATATT+TGG - chr1_3:39435256-39435275 Msa0117780:CDS 30.0%
AAAGAATCCTTGGGATATTT+GGG - chr1_3:39435257-39435276 Msa0117780:CDS 30.0%
! TTAAGGTAGTGAAGAAGTAT+AGG + chr1_3:39435320-39435339 None:intergenic 30.0%
TCAAAAGTAGTTCGTTCTAT+TGG - chr1_3:39435366-39435385 Msa0117780:intron 30.0%
!!! GAAAACATTTTTTCACACCA+AGG + chr1_3:39435458-39435477 None:intergenic 30.0%
!! TGTTTTCGATTTGAGAATAG+TGG - chr1_3:39435471-39435490 Msa0117780:intron 30.0%
TTCTTATCAAAGATTTGTCG+AGG + chr1_3:39435536-39435555 None:intergenic 30.0%
TCTGCATAGAACATAATTGT+TGG + chr1_3:39435660-39435679 None:intergenic 30.0%
AGAAAGAATATTCATGCACA+AGG - chr1_3:39435683-39435702 Msa0117780:intron 30.0%
AATGGATACATTTCAAGATC+CGG - chr1_3:39435798-39435817 Msa0117780:intron 30.0%
! GCATTCTTGATTTGATATGT+AGG - chr1_3:39435849-39435868 Msa0117780:intron 30.0%
TGTAGGACTTACAAATTTGT+TGG + chr1_3:39436046-39436065 None:intergenic 30.0%
!!! AACGAGTCTAGTTTTTTGAT+GGG + chr1_3:39436338-39436357 None:intergenic 30.0%
!!! TAACGAGTCTAGTTTTTTGA+TGG + chr1_3:39436339-39436358 None:intergenic 30.0%
TGATGAAGTTCGACTATATT+TGG + chr1_3:39436515-39436534 None:intergenic 30.0%
!! CAGGTTGAACTATTTGATTT+TGG + chr1_3:39436679-39436698 None:intergenic 30.0%
ATTGTGGACAAAGAAAATGA+TGG - chr1_3:39436728-39436747 Msa0117780:CDS 30.0%
TCTCAATTCTTCTTCAAGTA+AGG - chr1_3:39435119-39435138 Msa0117780:CDS 30.0%
!! TGTTATGGATTTTGTTGTAG+AGG + chr1_3:39435185-39435204 None:intergenic 30.0%
TCAAGAATGGTGATATAGTT+GGG + chr1_3:39435216-39435235 None:intergenic 30.0%
TTCAAGAATGGTGATATAGT+TGG + chr1_3:39435217-39435236 None:intergenic 30.0%
AATATCCCAAGGATTCTTTA+GGG + chr1_3:39435256-39435275 None:intergenic 30.0%
AAATATCCCAAGGATTCTTT+AGG + chr1_3:39435257-39435276 None:intergenic 30.0%
TAAAGAATCCTTGGGATATT+TGG - chr1_3:39435256-39435275 Msa0117780:CDS 30.0%
AAAGAATCCTTGGGATATTT+GGG - chr1_3:39435257-39435276 Msa0117780:CDS 30.0%
! TTAAGGTAGTGAAGAAGTAT+AGG + chr1_3:39435320-39435339 None:intergenic 30.0%
TCAAAAGTAGTTCGTTCTAT+TGG - chr1_3:39435366-39435385 Msa0117780:intron 30.0%
!!! GAAAACATTTTTTCACACCA+AGG + chr1_3:39435458-39435477 None:intergenic 30.0%
!! TGTTTTCGATTTGAGAATAG+TGG - chr1_3:39435471-39435490 Msa0117780:intron 30.0%
TTCTTATCAAAGATTTGTCG+AGG + chr1_3:39435536-39435555 None:intergenic 30.0%
TCTGCATAGAACATAATTGT+TGG + chr1_3:39435660-39435679 None:intergenic 30.0%
AGAAAGAATATTCATGCACA+AGG - chr1_3:39435683-39435702 Msa0117780:intron 30.0%
AATGGATACATTTCAAGATC+CGG - chr1_3:39435798-39435817 Msa0117780:intron 30.0%
! GCATTCTTGATTTGATATGT+AGG - chr1_3:39435849-39435868 Msa0117780:intron 30.0%
TGTAGGACTTACAAATTTGT+TGG + chr1_3:39436046-39436065 None:intergenic 30.0%
!!! AACGAGTCTAGTTTTTTGAT+GGG + chr1_3:39436338-39436357 None:intergenic 30.0%
!!! TAACGAGTCTAGTTTTTTGA+TGG + chr1_3:39436339-39436358 None:intergenic 30.0%
TGATGAAGTTCGACTATATT+TGG + chr1_3:39436515-39436534 None:intergenic 30.0%
!! CAGGTTGAACTATTTGATTT+TGG + chr1_3:39436679-39436698 None:intergenic 30.0%
ATTGTGGACAAAGAAAATGA+TGG - chr1_3:39436728-39436747 Msa0117780:CDS 30.0%
!! AGATTTCTTCTCTAGGGTTA+AGG + chr1_3:39435337-39435356 None:intergenic 35.0%
AGTGGTAGATTTCTTCTCTA+GGG + chr1_3:39435343-39435362 None:intergenic 35.0%
TAGTGGTAGATTTCTTCTCT+AGG + chr1_3:39435344-39435363 None:intergenic 35.0%
GTAGTTCGTTCTATTGGAAT+TGG - chr1_3:39435372-39435391 Msa0117780:intron 35.0%
TTCTATTGGAATTGGTGCAT+GGG - chr1_3:39435380-39435399 Msa0117780:intron 35.0%
AAAAACAAGAACCAACTCCT+TGG - chr1_3:39435438-39435457 Msa0117780:intron 35.0%
!!! ATTTTTTCACACCAAGGAGT+TGG + chr1_3:39435452-39435471 None:intergenic 35.0%
TGTATCACATTGCTTACCAA+AGG + chr1_3:39435561-39435580 None:intergenic 35.0%
CAAGTAACTTACAGATATGG+TGG + chr1_3:39435747-39435766 None:intergenic 35.0%
GAACCAAGAAACGAATCAAA+TGG - chr1_3:39435910-39435929 Msa0117780:intron 35.0%
CATAGTGTAATCCTTTGACA+TGG + chr1_3:39436541-39436560 None:intergenic 35.0%
! AGAATGCAAGAGCACAATAA+GGG + chr1_3:39436636-39436655 None:intergenic 35.0%
!! GCTCTTGCATTCTCATTTTT+AGG - chr1_3:39436643-39436662 Msa0117780:CDS 35.0%
TCATCATCATCTTCATTGTC+AGG + chr1_3:39436698-39436717 None:intergenic 35.0%
! GACAATGAAGATGATGATGA+TGG - chr1_3:39436698-39436717 Msa0117780:CDS 35.0%
!! AAATGATGGTGAAAGCATTG+TGG - chr1_3:39436742-39436761 Msa0117780:CDS 35.0%
GAGCTGATAGAGATTAATGA+AGG - chr1_3:39436815-39436834 Msa0117780:CDS 35.0%
!! AGATTTCTTCTCTAGGGTTA+AGG + chr1_3:39435337-39435356 None:intergenic 35.0%
AGTGGTAGATTTCTTCTCTA+GGG + chr1_3:39435343-39435362 None:intergenic 35.0%
TAGTGGTAGATTTCTTCTCT+AGG + chr1_3:39435344-39435363 None:intergenic 35.0%
GTAGTTCGTTCTATTGGAAT+TGG - chr1_3:39435372-39435391 Msa0117780:intron 35.0%
TTCTATTGGAATTGGTGCAT+GGG - chr1_3:39435380-39435399 Msa0117780:intron 35.0%
AAAAACAAGAACCAACTCCT+TGG - chr1_3:39435438-39435457 Msa0117780:intron 35.0%
!!! ATTTTTTCACACCAAGGAGT+TGG + chr1_3:39435452-39435471 None:intergenic 35.0%
TGTATCACATTGCTTACCAA+AGG + chr1_3:39435561-39435580 None:intergenic 35.0%
CAAGTAACTTACAGATATGG+TGG + chr1_3:39435747-39435766 None:intergenic 35.0%
GAACCAAGAAACGAATCAAA+TGG - chr1_3:39435910-39435929 Msa0117780:intron 35.0%
CATAGTGTAATCCTTTGACA+TGG + chr1_3:39436541-39436560 None:intergenic 35.0%
! AGAATGCAAGAGCACAATAA+GGG + chr1_3:39436636-39436655 None:intergenic 35.0%
!! GCTCTTGCATTCTCATTTTT+AGG - chr1_3:39436643-39436662 Msa0117780:CDS 35.0%
TCATCATCATCTTCATTGTC+AGG + chr1_3:39436698-39436717 None:intergenic 35.0%
! GACAATGAAGATGATGATGA+TGG - chr1_3:39436698-39436717 Msa0117780:CDS 35.0%
!! AAATGATGGTGAAAGCATTG+TGG - chr1_3:39436742-39436761 Msa0117780:CDS 35.0%
GAGCTGATAGAGATTAATGA+AGG - chr1_3:39436815-39436834 Msa0117780:CDS 35.0%
!! AGTTGGGCAGAGATTTGTTA+TGG + chr1_3:39435200-39435219 None:intergenic 40.0%
TTGGTACCCTAAAGAATCCT+TGG - chr1_3:39435247-39435266 Msa0117780:CDS 40.0%
TGGTACCCTAAAGAATCCTT+GGG - chr1_3:39435248-39435267 Msa0117780:CDS 40.0%
AACTCTTCCCAAATATCCCA+AGG + chr1_3:39435267-39435286 None:intergenic 40.0%
TGGGATATTTGGGAAGAGTT+TGG - chr1_3:39435267-39435286 Msa0117780:CDS 40.0%
!! ACGAACTACTTTTGAGCTAG+TGG + chr1_3:39435361-39435380 None:intergenic 40.0%
GTTCTATTGGAATTGGTGCA+TGG - chr1_3:39435379-39435398 Msa0117780:intron 40.0%
AATAGTGGCACTTACCATGA+TGG - chr1_3:39435486-39435505 Msa0117780:intron 40.0%
GAAAGAAAGACCATTGTTCC+TGG - chr1_3:39435710-39435729 Msa0117780:intron 40.0%
! CCACCATTTGATTCGTTTCT+TGG + chr1_3:39435916-39435935 None:intergenic 40.0%
CCAAGAAACGAATCAAATGG+TGG - chr1_3:39435913-39435932 Msa0117780:intron 40.0%
!! GAATCAAATGGTGGACACAA+TGG - chr1_3:39435922-39435941 Msa0117780:intron 40.0%
TGGACACAATGGCACAAATA+TGG - chr1_3:39435933-39435952 Msa0117780:intron 40.0%
CTTCATCACATCCATGTCAA+AGG - chr1_3:39436527-39436546 Msa0117780:CDS 40.0%
! GAGAATGCAAGAGCACAATA+AGG + chr1_3:39436637-39436656 None:intergenic 40.0%
!! TGATGATGGTGAATGCATTG+TGG - chr1_3:39436712-39436731 Msa0117780:CDS 40.0%
! ATGATGATGCTGGTATGTCT+TGG - chr1_3:39436774-39436793 Msa0117780:CDS 40.0%
! TTGGCCTGAGATTTTTGCAA+AGG - chr1_3:39436793-39436812 Msa0117780:CDS 40.0%
AGCTCCTTTGCAAAAATCTC+AGG + chr1_3:39436800-39436819 None:intergenic 40.0%
! TGAAGGATGCTTGTTAGAAG+AGG - chr1_3:39436832-39436851 Msa0117780:CDS 40.0%
! ATGCTTGTTAGAAGAGGAAG+AGG - chr1_3:39436838-39436857 Msa0117780:CDS 40.0%
!! AGTTGGGCAGAGATTTGTTA+TGG + chr1_3:39435200-39435219 None:intergenic 40.0%
TTGGTACCCTAAAGAATCCT+TGG - chr1_3:39435247-39435266 Msa0117780:CDS 40.0%
TGGTACCCTAAAGAATCCTT+GGG - chr1_3:39435248-39435267 Msa0117780:CDS 40.0%
AACTCTTCCCAAATATCCCA+AGG + chr1_3:39435267-39435286 None:intergenic 40.0%
TGGGATATTTGGGAAGAGTT+TGG - chr1_3:39435267-39435286 Msa0117780:CDS 40.0%
!! ACGAACTACTTTTGAGCTAG+TGG + chr1_3:39435361-39435380 None:intergenic 40.0%
GTTCTATTGGAATTGGTGCA+TGG - chr1_3:39435379-39435398 Msa0117780:intron 40.0%
AATAGTGGCACTTACCATGA+TGG - chr1_3:39435486-39435505 Msa0117780:intron 40.0%
GAAAGAAAGACCATTGTTCC+TGG - chr1_3:39435710-39435729 Msa0117780:intron 40.0%
! CCACCATTTGATTCGTTTCT+TGG + chr1_3:39435916-39435935 None:intergenic 40.0%
CCAAGAAACGAATCAAATGG+TGG - chr1_3:39435913-39435932 Msa0117780:intron 40.0%
!! GAATCAAATGGTGGACACAA+TGG - chr1_3:39435922-39435941 Msa0117780:intron 40.0%
TGGACACAATGGCACAAATA+TGG - chr1_3:39435933-39435952 Msa0117780:intron 40.0%
CTTCATCACATCCATGTCAA+AGG - chr1_3:39436527-39436546 Msa0117780:CDS 40.0%
! GAGAATGCAAGAGCACAATA+AGG + chr1_3:39436637-39436656 None:intergenic 40.0%
!! TGATGATGGTGAATGCATTG+TGG - chr1_3:39436712-39436731 Msa0117780:CDS 40.0%
! ATGATGATGCTGGTATGTCT+TGG - chr1_3:39436774-39436793 Msa0117780:CDS 40.0%
! TTGGCCTGAGATTTTTGCAA+AGG - chr1_3:39436793-39436812 Msa0117780:CDS 40.0%
AGCTCCTTTGCAAAAATCTC+AGG + chr1_3:39436800-39436819 None:intergenic 40.0%
! TGAAGGATGCTTGTTAGAAG+AGG - chr1_3:39436832-39436851 Msa0117780:CDS 40.0%
! ATGCTTGTTAGAAGAGGAAG+AGG - chr1_3:39436838-39436857 Msa0117780:CDS 40.0%
ATTGGAATTGGTGCATGGGA+AGG - chr1_3:39435384-39435403 Msa0117780:intron 45.0%
CCAAGAATGGTTGTCTCTCT+TGG + chr1_3:39436012-39436031 None:intergenic 45.0%
CCAAGAGAGACAACCATTCT+TGG - chr1_3:39436009-39436028 Msa0117780:CDS 45.0%
! GACGAAGAAGATGATGATGC+TGG - chr1_3:39436764-39436783 Msa0117780:CDS 45.0%
ATTGGAATTGGTGCATGGGA+AGG - chr1_3:39435384-39435403 Msa0117780:intron 45.0%
CCAAGAATGGTTGTCTCTCT+TGG + chr1_3:39436012-39436031 None:intergenic 45.0%
CCAAGAGAGACAACCATTCT+TGG - chr1_3:39436009-39436028 Msa0117780:CDS 45.0%
! GACGAAGAAGATGATGATGC+TGG - chr1_3:39436764-39436783 Msa0117780:CDS 45.0%
TGGGAAGGTGAAGAAGACAC+TGG - chr1_3:39435399-39435418 Msa0117780:intron 50.0%
! GCACTTACCATGATGGTGCA+TGG - chr1_3:39435493-39435512 Msa0117780:intron 50.0%
GCAAGATCCATGCACCATCA+TGG + chr1_3:39435503-39435522 None:intergenic 50.0%
TTCTGCACGTCCAGGAACAA+TGG + chr1_3:39435723-39435742 None:intergenic 50.0%
TGGGAAGGTGAAGAAGACAC+TGG - chr1_3:39435399-39435418 Msa0117780:intron 50.0%
! GCACTTACCATGATGGTGCA+TGG - chr1_3:39435493-39435512 Msa0117780:intron 50.0%
GCAAGATCCATGCACCATCA+TGG + chr1_3:39435503-39435522 None:intergenic 50.0%
TTCTGCACGTCCAGGAACAA+TGG + chr1_3:39435723-39435742 None:intergenic 50.0%
ATGGTGGTTTCTGCACGTCC+AGG + chr1_3:39435731-39435750 None:intergenic 55.0%
GGCTGCTTCGAGTCCAAGAA+TGG + chr1_3:39436025-39436044 None:intergenic 55.0%
ATGGTGGTTTCTGCACGTCC+AGG + chr1_3:39435731-39435750 None:intergenic 55.0%
GGCTGCTTCGAGTCCAAGAA+TGG + chr1_3:39436025-39436044 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 39435114 39436922 39435114 ID=Msa0117780;Name=Msa0117780
chr1_3 mRNA 39435114 39436922 39435114 ID=Msa0117780-mRNA-1;Parent=Msa0117780;Name=Msa0117780-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|4|0|329
chr1_3 exon 39436473 39436922 39436473 ID=Msa0117780-mRNA-1:exon:12188;Parent=Msa0117780-mRNA-1
chr1_3 exon 39436282 39436380 39436282 ID=Msa0117780-mRNA-1:exon:12187;Parent=Msa0117780-mRNA-1
chr1_3 exon 39435983 39436165 39435983 ID=Msa0117780-mRNA-1:exon:12186;Parent=Msa0117780-mRNA-1
chr1_3 exon 39435114 39435371 39435114 ID=Msa0117780-mRNA-1:exon:12185;Parent=Msa0117780-mRNA-1
chr1_3 CDS 39436473 39436922 39436473 ID=Msa0117780-mRNA-1:cds;Parent=Msa0117780-mRNA-1
chr1_3 CDS 39436282 39436380 39436282 ID=Msa0117780-mRNA-1:cds;Parent=Msa0117780-mRNA-1
chr1_3 CDS 39435983 39436165 39435983 ID=Msa0117780-mRNA-1:cds;Parent=Msa0117780-mRNA-1
chr1_3 CDS 39435114 39435371 39435114 ID=Msa0117780-mRNA-1:cds;Parent=Msa0117780-mRNA-1
Gene Sequence

>Msa0117780

ATGGCTCTCAATTCTTCTTCAAGTAAGGATTTCAAATTCACACTCAGAGATGATGAACTTCTACGTTTCCTCTACAACAAAATCCATAACAAATCTCTGCCCAACTATATCACCATTCTTGAATACGATTTGTTTGGTACCCTAAAGAATCCTTGGGATATTTGGGAAGAGTTTGGAGCTTCTCGCTCATATTGTGAAAAAGACCTATACTTCTTCACTACCTTAACCCTAGAGAAGAAATCTACCACTAGCTCAAAAGTAGTTCGTTCTATTGGAATTGGTGCATGGGAAGGTGAAGAAGACACTGGAAAAATTATAGTTGCAAAAAACAAGAACCAACTCCTTGGTGTGAAAAAATGTTTTCGATTTGAGAATAGTGGCACTTACCATGATGGTGCATGGATCTTGCATGAATATAGCCTCGACAAATCTTTGATAAGAAATCCTTTGGCCAACAATTATGTTCTATGCAGATTTAGAAAGAATATTCATGCACAAGGTAAAGAAAGAAAGACCATTGTTCCTGGACGTGCAGAAACCACCATATCTGGAAATAAAGATTCGTTGACAAAATATATAATTTATAAAGAACCAAGAAACGAATCAAATGGTGGACACAATGGCACAAATATGGTTAATACAGAGTCTCATTATAAGAAAGAAGAAAAAGATGTTAGAAATATTTGGCCAAGAGAGACAACCATTCTTGGACTCGAAGCAGCCAACAAATTTGAGAATATCGACCAAAATCAAATAGTTCAACCTGACAATGAAGATGATGATGATGGTGAATGCATTGTGGACAAAGAAAATGATGGTGAAAGCATTGTGGACGAAGAAGATGATGATGCTGGTATGTCTTGGCCTGAGATTTTTGCAAAGGAGCTGATAGAGATTAATGAAGGATGCTTGTTAGAAGAGGAAGAGGTTCAAATGATTCCCAATAAGTTTCATGAAGAGTTATTATTGGATAATACTAACATAAAATAA
Protein sequence

>Msa0117780

MALNSSSSKDFKFTLRDDELLRFLYNKIHNKSLPNYITILEYDLFGTLKNPWDIWEEFGASRSYCEKDLYFFTTLTLEKKSTTSSKVVRSIGIGAWEGEEDTGKIIVAKNKNQLLGVKKCFRFENSGTYHDGAWILHEYSLDKSLIRNPLANNYVLCRFRKNIHAQGKERKTIVPGRAETTISGNKDSLTKYIIYKEPRNESNGGHNGTNMVNTESHYKKEEKDVRNIWPRETTILGLEAANKFENIDQNQIVQPDNEDDDDGECIVDKENDGESIVDEEDDDAGMSWPEIFAKELIEINEGCLLEEEEVQMIPNKFHEELLLDNTNIK*