AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0117930

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0117930 MtrunA17_Chr1g0175071 93.125 320 20 1 1 320 1 318 0.0 603
Msa0117930 MtrunA17_Chr7g0258531 45.126 277 120 9 53 315 90 348 3.16e-54 181
Msa0117930 MtrunA17_Chr1g0175091 51.852 135 64 1 175 309 254 387 4.14e-35 132
Msa0117930 MtrunA17_Chr7g0258551 51.145 131 64 0 179 309 212 342 5.10e-34 128
Msa0117930 MtrunA17_Chr1g0190301 37.727 220 129 4 91 309 94 306 2.39e-33 126
Msa0117930 MtrunA17_Chr7g0261911 44.667 150 75 3 160 307 248 391 2.04e-32 125
Msa0117930 MtrunA17_Chr2g0291021 45.588 136 67 2 179 309 49 182 9.05e-29 113
Msa0117930 MtrunA17_Chr7g0274471 43.939 132 73 1 179 309 218 349 3.67e-28 113
Msa0117930 MtrunA17_Chr7g0254981 39.855 138 79 1 176 309 47 184 1.45e-26 105
Msa0117930 MtrunA17_Chr8g0356501 38.608 158 85 2 168 317 26 179 1.10e-24 102
Msa0117930 MtrunA17_Chr2g0310011 40.845 142 72 2 168 301 24 161 6.01e-24 100
Msa0117930 MtrunA17_Chr2g0321461 41.615 161 73 6 167 306 33 193 9.41e-24 100
Msa0117930 MtrunA17_Chr6g0461641 38.562 153 77 3 174 309 37 189 1.00e-23 100
Msa0117930 MtrunA17_Chr5g0417521 37.584 149 74 3 172 304 8 153 2.07e-20 90.5
Msa0117930 MtrunA17_Chr5g0409731 36.145 166 79 4 174 319 20 178 5.80e-20 88.2
Msa0117930 MtrunA17_Chr4g0042451 37.931 145 74 3 174 304 12 154 7.08e-20 88.6
Msa0117930 MtrunA17_Chr7g0242521 37.857 140 84 1 175 311 9 148 2.79e-18 82.4
Msa0117930 MtrunA17_Chr2g0290991 34.266 143 81 2 174 303 34 176 8.87e-18 82.4
Msa0117930 MtrunA17_Chr1g0197171 33.103 145 94 3 166 309 31 173 2.57e-16 77.8
Msa0117930 MtrunA17_Chr8g0379121 51.429 70 26 1 181 250 113 174 1.68e-14 73.9
Msa0117930 MtrunA17_Chr1g0162931 62.264 53 20 0 181 233 20 72 2.04e-14 72.4
Msa0117930 MtrunA17_Chr1g0175081 59.615 52 21 0 181 232 24 75 2.86e-14 67.8
Msa0117930 MtrunA17_Chr2g0320231 51.429 70 26 1 181 250 99 160 4.61e-14 72.0
Msa0117930 MtrunA17_Chr3g0095611 59.259 54 22 0 181 234 21 74 6.75e-14 69.7
Msa0117930 MtrunA17_Chr1g0210911 60.377 53 21 0 181 233 24 76 8.19e-14 71.2
Msa0117930 MtrunA17_Chr8g0366281 45.783 83 40 1 157 234 146 228 1.04e-13 70.9
Msa0117930 MtrunA17_Chr7g0243081 56.604 53 23 0 181 233 73 125 4.81e-13 68.9
Msa0117930 MtrunA17_Chr6g0479431 50.847 59 29 0 181 239 204 262 6.26e-13 68.9
Msa0117930 MtrunA17_Chr1g0154261 47.368 76 36 2 181 255 128 200 1.11e-12 67.4
Msa0117930 MtrunA17_Chr3g0109981 38.532 109 51 3 181 289 276 368 1.18e-12 68.2
Msa0117930 MtrunA17_Chr5g0410741 39.535 86 47 1 181 261 221 306 1.49e-12 67.8
Msa0117930 MtrunA17_Chr2g0283951 50.000 74 25 2 181 250 91 156 2.49e-12 67.0
Msa0117930 MtrunA17_Chr2g0288601 57.143 49 21 0 181 229 73 121 5.33e-12 62.4
Msa0117930 MtrunA17_Chr7g0275401 34.127 126 54 2 138 235 123 247 2.55e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0117930 AT5G06800.1 42.941 170 94 1 140 309 156 322 5.80e-35 130
Msa0117930 AT5G06800.2 41.714 175 94 2 140 309 156 327 3.66e-33 126
Msa0117930 AT4G28610.1 46.528 144 72 2 166 307 215 355 1.30e-31 122
Msa0117930 AT2G01060.1 48.175 137 61 2 179 309 15 147 4.01e-30 116
Msa0117930 AT5G29000.2 43.165 139 75 2 175 309 227 365 5.50e-29 115
Msa0117930 AT5G29000.1 43.165 139 75 2 175 309 184 322 5.56e-29 114
Msa0117930 AT5G29000.3 43.165 139 75 2 175 309 184 322 5.56e-29 114
Msa0117930 AT3G04450.4 30.800 250 141 5 88 315 99 338 7.90e-28 112
Msa0117930 AT3G04450.2 30.800 250 141 5 88 315 99 338 7.90e-28 112
Msa0117930 AT3G04450.1 40.426 141 80 1 179 315 238 378 1.06e-27 112
Msa0117930 AT3G13040.3 43.262 141 74 2 175 309 237 377 1.83e-24 103
Msa0117930 AT3G13040.1 43.262 141 74 2 175 309 237 377 1.83e-24 103
Msa0117930 AT3G13040.2 43.262 141 74 2 175 309 237 377 1.83e-24 103
Msa0117930 AT4G13640.1 38.217 157 84 3 174 317 32 188 7.24e-24 99.4
Msa0117930 AT1G79430.2 38.994 159 81 3 168 316 23 175 2.03e-23 99.4
Msa0117930 AT2G20400.2 41.985 131 74 1 179 307 231 361 5.98e-23 98.6
Msa0117930 AT3G24120.1 39.490 157 82 2 174 317 36 192 6.30e-23 97.1
Msa0117930 AT5G18240.1 37.821 156 81 2 167 306 33 188 1.47e-22 97.4
Msa0117930 AT5G18240.4 37.821 156 81 2 167 306 33 188 1.47e-22 97.4
Msa0117930 AT3G04030.3 36.145 166 72 3 166 306 32 188 1.52e-22 97.4
Msa0117930 AT2G20400.1 42.748 131 72 2 179 307 231 360 1.66e-22 97.4
Msa0117930 AT4G13640.2 37.500 160 84 4 174 317 32 191 7.27e-22 94.0
Msa0117930 AT3G24120.2 38.750 160 82 3 174 317 36 195 5.58e-21 91.7
Msa0117930 AT5G18240.5 37.179 156 80 3 167 306 33 186 5.15e-20 90.1
Msa0117930 AT3G04030.2 34.940 166 73 4 166 306 32 187 1.19e-19 89.0
Msa0117930 AT1G69580.1 36.538 156 67 3 174 304 29 177 3.03e-19 87.4
Msa0117930 AT3G12730.1 34.459 148 88 2 168 306 12 159 1.03e-18 84.0
Msa0117930 AT1G69580.2 35.065 154 73 2 174 304 29 178 1.74e-18 85.1
Msa0117930 AT5G18240.2 34.615 156 80 3 167 306 33 182 9.27e-18 83.6
Msa0117930 AT5G18240.3 34.615 156 80 3 167 306 33 182 9.27e-18 83.6
Msa0117930 AT3G04030.1 31.928 166 73 3 166 306 32 182 9.70e-18 83.6
Msa0117930 AT5G29000.4 58.333 60 25 0 175 234 227 286 5.79e-17 80.5
Msa0117930 AT5G45580.2 31.515 165 84 3 169 309 13 172 1.89e-16 78.6
Msa0117930 AT5G06800.3 45.161 93 48 1 140 232 156 245 2.82e-16 78.2
Msa0117930 AT5G45580.1 31.875 160 80 3 174 309 2 156 3.43e-16 77.4
Msa0117930 AT3G04450.3 55.172 58 26 0 179 236 238 295 4.91e-16 77.8
Msa0117930 AT2G42660.1 58.491 53 22 0 181 233 52 104 1.39e-14 72.8
Msa0117930 AT5G42630.2 43.956 91 47 2 161 250 86 173 1.71e-14 72.0
Msa0117930 AT5G42630.1 43.956 91 47 2 161 250 86 173 1.97e-14 72.8
Msa0117930 AT4G04605.1 60.377 53 21 0 181 233 16 68 2.00e-14 71.6
Msa0117930 AT2G40260.1 53.623 69 30 1 181 247 84 152 2.56e-14 73.6
Msa0117930 AT4G04580.1 58.491 53 22 0 181 233 16 68 5.41e-14 69.3
Msa0117930 AT1G14600.1 61.111 54 21 0 181 234 25 78 5.67e-14 71.2
Msa0117930 AT5G16560.1 41.111 90 53 0 181 270 221 310 6.09e-14 72.4
Msa0117930 AT2G01060.2 45.098 102 46 2 214 309 1 98 1.85e-13 69.3
Msa0117930 AT2G38300.1 46.575 73 31 1 181 253 56 120 3.39e-13 69.7
Msa0117930 AT1G32240.2 38.095 105 59 2 181 279 215 319 3.93e-13 69.7
Msa0117930 AT1G32240.1 38.095 105 59 2 181 279 215 319 4.85e-13 69.7
Msa0117930 AT2G02060.2 58.491 53 22 0 181 233 31 83 8.68e-13 67.8
Msa0117930 AT2G02060.1 58.491 53 22 0 181 233 31 83 8.68e-13 67.8
Msa0117930 AT4G17695.1 50.000 70 32 2 173 239 155 224 1.16e-12 68.2
Msa0117930 AT2G06020.1 48.276 58 30 0 175 232 82 139 1.72e-12 67.4
Msa0117930 AT4G04555.1 58.824 51 21 0 183 233 18 68 3.99e-12 62.4
Msa0117930 AT4G37180.1 45.333 75 40 1 164 238 196 269 2.12e-11 64.7
Msa0117930 AT4G37180.2 45.333 75 40 1 164 238 203 276 2.39e-11 64.3

Find 56 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAACAACCTGCTTCAAAT+TGG 0.228288 1_3:+40015572 Msa0117930:CDS
AAAGGTGAATCTGTTGTTAT+TGG 0.269136 1_3:-40015492 None:intergenic
CAGCCTTGACAATACATAAA+AGG 0.269493 1_3:-40015510 None:intergenic
ATGAGTTTGACATACCTATT+TGG 0.292306 1_3:+40015451 Msa0117930:CDS
TATCTTAAAATCAGAGTTGC+TGG 0.350060 1_3:-40016692 None:intergenic
TCATGTCAAAAGTCATTTGC+AGG 0.353917 1_3:+40016951 Msa0117930:CDS
GTGATTCTTCTATTTGCATT+TGG 0.358926 1_3:-40017999 None:intergenic
CTAAAATGTACATAAATCAT+TGG 0.360570 1_3:+40015301 None:intergenic
TTCCCTCTTGTTCTGATAAG+AGG 0.378026 1_3:+40015658 Msa0117930:CDS
CCCAATTTGAAGCAGGTTGT+TGG 0.384228 1_3:-40015573 None:intergenic
TCTACATTACACACGCATAA+AGG 0.388323 1_3:+40017095 Msa0117930:CDS
GTAAATTCATTGCTCCAAAT+AGG 0.396813 1_3:-40015465 None:intergenic
TAAAATGTACATAAATCATT+GGG 0.405097 1_3:+40015302 None:intergenic
CCAACAACCTGCTTCAAATT+GGG 0.416004 1_3:+40015573 Msa0117930:CDS
ATTTCATGGGACCTATGATC+TGG 0.435142 1_3:-40015702 None:intergenic
GACATATCAGCAATAATTCA+TGG 0.436755 1_3:+40015375 Msa0117930:CDS
ATTATATAAACTTACTTTCA+GGG 0.437925 1_3:-40016795 None:intergenic
ACAAAAGAAAAGAACCAAAC+AGG 0.442809 1_3:+40018392 Msa0117930:CDS
ATCATTTGTAGTATAGCCTT+AGG 0.450838 1_3:-40016904 None:intergenic
TAAGGCTATACTACAAATGA+TGG 0.452684 1_3:+40016906 Msa0117930:CDS
ACAAACAATCTTCACCTTGT+GGG 0.458647 1_3:+40016653 Msa0117930:CDS
ATTATGATTGTTAACCAGCT+TGG 0.463406 1_3:+40016769 Msa0117930:CDS
TTGAGCTTGAAAATTCATAT+AGG 0.463644 1_3:-40015415 None:intergenic
TCCCTCTTGTTCTGATAAGA+GGG 0.481140 1_3:+40015659 Msa0117930:CDS
TTCAGCACCCAATTTGAAGC+AGG 0.483419 1_3:-40015580 None:intergenic
TACTTTCAGGGCCACCAAGC+TGG 0.490074 1_3:-40016783 None:intergenic
ACACACGCATAAAGGTTTGA+AGG 0.499011 1_3:+40017103 Msa0117930:CDS
TTGTTCTGATAAGAGGGTGC+TGG 0.508794 1_3:+40015665 Msa0117930:CDS
TTTCATGGGACCTATGATCT+GGG 0.511209 1_3:-40015701 None:intergenic
GATGCAGCGAAATTTGCAAC+TGG 0.519384 1_3:+40018326 Msa0117930:intron
ATGATTGTTAACCAGCTTGG+TGG 0.519893 1_3:+40016772 Msa0117930:CDS
TTTCTGAAAGGAAATTTCAT+GGG 0.532722 1_3:-40015715 None:intergenic
AGGGTAAAAGAAGATTAAGA+TGG 0.538823 1_3:+40016722 Msa0117930:CDS
AGAGGACTAGCTATGCTACA+AGG 0.548072 1_3:-40015348 None:intergenic
GAGGAAGGACAAATAACAGA+TGG 0.548655 1_3:+40017813 Msa0117930:CDS
AATCACAGAGGCAAAGCCTA+AGG 0.564212 1_3:+40016888 Msa0117930:intron
GACAAACAATCTTCACCTTG+TGG 0.566856 1_3:+40016652 Msa0117930:CDS
GAGCAATCAACGACAATTCG+AGG 0.567446 1_3:+40018047 Msa0117930:CDS
AACTACAACTTGAGGTTGAG+AGG 0.574473 1_3:+40018025 Msa0117930:CDS
CACCCTCTTATCAGAACAAG+AGG 0.577698 1_3:-40015661 None:intergenic
CACACGCATAAAGGTTTGAA+GGG 0.581415 1_3:+40017104 Msa0117930:CDS
AATTTCCTTTCAGAAAACTC+AGG 0.594791 1_3:+40015722 Msa0117930:CDS
CATTGTTGCAGGAGTATCTG+AGG 0.605886 1_3:+40017794 Msa0117930:intron
AAGGCTATACTACAAATGAT+GGG 0.610409 1_3:+40016907 Msa0117930:CDS
ATTGCTGATATGTCCTCTAG+AGG 0.610808 1_3:-40015366 None:intergenic
AGGAAGGACAAATAACAGAT+GGG 0.613736 1_3:+40017814 Msa0117930:CDS
TGAATCTGTTGTTATTGGCA+TGG 0.626242 1_3:-40015487 None:intergenic
GAGGACTAGCTATGCTACAA+GGG 0.630241 1_3:-40015347 None:intergenic
TAGCATAGCTAGTCCTCTAG+AGG 0.637217 1_3:+40015353 Msa0117930:CDS
ATCACGACAACTACAACTTG+AGG 0.638258 1_3:+40018017 Msa0117930:CDS
GTTGCAGGAGTATCTGAGGA+AGG 0.642276 1_3:+40017798 Msa0117930:intron
TTCATGGGACCTATGATCTG+GGG 0.644180 1_3:-40015700 None:intergenic
TATACTACAAATGATGGGTG+TGG 0.647886 1_3:+40016912 Msa0117930:CDS
GTATGTCAAACTCATGCTGA+TGG 0.650055 1_3:-40015443 None:intergenic
ACCCTCTTATCAGAACAAGA+GGG 0.715933 1_3:-40015660 None:intergenic
GTTGCAAAATCAAACCCACA+AGG 0.725049 1_3:-40016667 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAATGAAACAATTAT+TGG - chr1_3:40016033-40016052 None:intergenic 10.0%
!! AATAAAAATAAAAATAGAGT+AGG + chr1_3:40017375-40017394 Msa0117930:intron 10.0%
!! ATAAAAATAAAAATAGAGTA+GGG + chr1_3:40017376-40017395 Msa0117930:intron 10.0%
!! ATAATTAACAAAAAATAAGA+AGG - chr1_3:40017495-40017514 None:intergenic 10.0%
!! AATATTTGACAAAAAAAAAT+GGG - chr1_3:40017566-40017585 None:intergenic 10.0%
!! AAATATTTGACAAAAAAAAA+TGG - chr1_3:40017567-40017586 None:intergenic 10.0%
!!! TTTTTATATACAAGTAAATA+TGG - chr1_3:40017721-40017740 None:intergenic 10.0%
!!! ATATTTTATATCAAGAATTA+AGG + chr1_3:40018143-40018162 Msa0117930:intron 10.0%
!! AAAGAGTCATATAAAAATTA+AGG - chr1_3:40016141-40016160 None:intergenic 15.0%
!!! ATTATTGATTACAATGAATT+TGG + chr1_3:40016189-40016208 Msa0117930:intron 15.0%
!!! TTATTGATTACAATGAATTT+GGG + chr1_3:40016190-40016209 Msa0117930:intron 15.0%
!! ATTATATAAACTTACTTTCA+GGG - chr1_3:40016798-40016817 None:intergenic 15.0%
!! AATTATATAAACTTACTTTC+AGG - chr1_3:40016799-40016818 None:intergenic 15.0%
!! ATTACTATTAGTATTATTGT+TGG - chr1_3:40017169-40017188 None:intergenic 15.0%
!!! TTTTTTTGTCAAATATTTAG+TGG + chr1_3:40017569-40017588 Msa0117930:intron 15.0%
!! TTAGAAATTCATGAATAAAT+GGG + chr1_3:40017592-40017611 Msa0117930:intron 15.0%
!! TAACTTCTTCAAAAATATTA+AGG + chr1_3:40018261-40018280 Msa0117930:intron 15.0%
!! AATTTGAAGAGTAATAAGAA+AGG - chr1_3:40015769-40015788 None:intergenic 20.0%
!!! ATTACTCTTCAAATTTTTGT+TGG + chr1_3:40015774-40015793 Msa0117930:intron 20.0%
!!! TAATAAAATGATACCCTTTT+AGG - chr1_3:40016244-40016263 None:intergenic 20.0%
!!! TCATCATACATATTTAAGAT+TGG - chr1_3:40016417-40016436 None:intergenic 20.0%
!! ATCTTAAATATGTATGATGA+CGG + chr1_3:40016417-40016436 Msa0117930:intron 20.0%
!! AATTATAAATAAGACCATAC+AGG - chr1_3:40017143-40017162 None:intergenic 20.0%
!! TCAAAACTGAAAACTTAATT+TGG - chr1_3:40017222-40017241 None:intergenic 20.0%
!! AGTAAAATATATTGAAGTGT+AGG - chr1_3:40017524-40017543 None:intergenic 20.0%
!! GTTAGAAATTCATGAATAAA+TGG + chr1_3:40017591-40017610 Msa0117930:intron 20.0%
!! TAGAAATTCATGAATAAATG+GGG + chr1_3:40017593-40017612 Msa0117930:intron 20.0%
!!! ATAATGCATTTTAATATGCA+GGG - chr1_3:40017640-40017659 None:intergenic 20.0%
!!! AATAATGCATTTTAATATGC+AGG - chr1_3:40017641-40017660 None:intergenic 20.0%
!! TAAATTGACATCAAGAATTT+AGG + chr1_3:40018089-40018108 Msa0117930:intron 20.0%
!! GAATTAAGGAAATATTATGT+TGG + chr1_3:40018157-40018176 Msa0117930:intron 20.0%
! TTGAGCTTGAAAATTCATAT+AGG - chr1_3:40015418-40015437 None:intergenic 25.0%
!! TTTCTGAAAGGAAATTTCAT+GGG - chr1_3:40015718-40015737 None:intergenic 25.0%
!!! TTTTCTGAAAGGAAATTTCA+TGG - chr1_3:40015719-40015738 None:intergenic 25.0%
! AACCATTGTTAATTAGTCAA+TGG + chr1_3:40015801-40015820 Msa0117930:intron 25.0%
!! ATCCATTGACTAATTAACAA+TGG - chr1_3:40015806-40015825 None:intergenic 25.0%
! ATTAGTCAATGGATGAATTT+AGG + chr1_3:40015812-40015831 Msa0117930:intron 25.0%
! TAATTGACTACAGTTTCAAT+AGG + chr1_3:40016165-40016184 Msa0117930:intron 25.0%
! TTAGGTATACATATTGCTTT+GGG - chr1_3:40016226-40016245 None:intergenic 25.0%
! TTTAGGTATACATATTGCTT+TGG - chr1_3:40016227-40016246 None:intergenic 25.0%
! AAGCAATATGTATACCTAAA+AGG + chr1_3:40016227-40016246 Msa0117930:intron 25.0%
! AGCAATATGTATACCTAAAA+GGG + chr1_3:40016228-40016247 Msa0117930:intron 25.0%
!!! CGGTTCAATTAATTTTAAGT+CGG + chr1_3:40016386-40016405 Msa0117930:intron 25.0%
!!! GTAGTTTTTGAAGGAATAAA+AGG + chr1_3:40016515-40016534 Msa0117930:intron 25.0%
! AAACTTCTAAATGGATCATT+GGG - chr1_3:40016551-40016570 None:intergenic 25.0%
! GTTACAAAGAAACTTCTAAA+TGG - chr1_3:40016560-40016579 None:intergenic 25.0%
! ACTATGATAAGACATTTATG+AGG - chr1_3:40016612-40016631 None:intergenic 25.0%
! TCATAAATGTCTTATCATAG+TGG + chr1_3:40016611-40016630 Msa0117930:intron 25.0%
! CATAAATGTCTTATCATAGT+GGG + chr1_3:40016612-40016631 Msa0117930:intron 25.0%
!!! TGATTTTAAGATAACTGCAA+AGG + chr1_3:40016702-40016721 Msa0117930:CDS 25.0%
!!! GATTTTAAGATAACTGCAAA+GGG + chr1_3:40016703-40016722 Msa0117930:CDS 25.0%
!! ATATAGTTTGCACTCAAAAT+AGG - chr1_3:40017019-40017038 None:intergenic 25.0%
! GGTTAAAATGCATTTCATAA+AGG - chr1_3:40017195-40017214 None:intergenic 25.0%
!! CTGAAAACTTAATTTGGTAT+TGG - chr1_3:40017216-40017235 None:intergenic 25.0%
! ACGATAATGTATAAGATTCT+TGG - chr1_3:40017459-40017478 None:intergenic 25.0%
! GTAATTTAAATCATTGTTGC+AGG + chr1_3:40017783-40017802 Msa0117930:intron 25.0%
!! TTGATAACCTTAAAATTGTG+TGG + chr1_3:40017876-40017895 Msa0117930:intron 25.0%
!! ATCTTAACCACACAATTTTA+AGG - chr1_3:40017886-40017905 None:intergenic 25.0%
! TGAATGTGAACAATTAAAGA+AGG - chr1_3:40017909-40017928 None:intergenic 25.0%
! CTTTAATTGTTCACATTCAT+TGG + chr1_3:40017910-40017929 Msa0117930:intron 25.0%
! TATTTCAGTATCAATTTGAG+TGG + chr1_3:40018231-40018250 Msa0117930:intron 25.0%
GACATATCAGCAATAATTCA+TGG + chr1_3:40015375-40015394 Msa0117930:CDS 30.0%
ATGAGTTTGACATACCTATT+TGG + chr1_3:40015451-40015470 Msa0117930:CDS 30.0%
GTAAATTCATTGCTCCAAAT+AGG - chr1_3:40015468-40015487 None:intergenic 30.0%
AAAGGTGAATCTGTTGTTAT+TGG - chr1_3:40015495-40015514 None:intergenic 30.0%
! TCACCTTTTATGTATTGTCA+AGG + chr1_3:40015507-40015526 Msa0117930:CDS 30.0%
AATTTCCTTTCAGAAAACTC+AGG + chr1_3:40015722-40015741 Msa0117930:CDS 30.0%
ATTTAGGAACACATTTGTCA+CGG + chr1_3:40015828-40015847 Msa0117930:intron 30.0%
TATCATATATGTTCCGCATT+GGG - chr1_3:40015865-40015884 None:intergenic 30.0%
ATATCATATATGTTCCGCAT+TGG - chr1_3:40015866-40015885 None:intergenic 30.0%
!!! TTTTGTTTCCTTAACTTGTG+TGG + chr1_3:40015897-40015916 Msa0117930:intron 30.0%
! GAAACAATTATTGGTTAGAG+AGG - chr1_3:40016024-40016043 None:intergenic 30.0%
! TTTGATTTCATTCGATAAGG+AGG - chr1_3:40016081-40016100 None:intergenic 30.0%
! GAGTTTGATTTCATTCGATA+AGG - chr1_3:40016084-40016103 None:intergenic 30.0%
!! TTGTTGGACATCTTTTAAAG+CGG + chr1_3:40016366-40016385 Msa0117930:intron 30.0%
!!! CTATCGTTTGATTTTTTCAG+CGG - chr1_3:40016450-40016469 None:intergenic 30.0%
GAAACTTCTAAATGGATCAT+TGG - chr1_3:40016552-40016571 None:intergenic 30.0%
TATCTTAAAATCAGAGTTGC+TGG - chr1_3:40016695-40016714 None:intergenic 30.0%
AGGGTAAAAGAAGATTAAGA+TGG + chr1_3:40016722-40016741 Msa0117930:CDS 30.0%
ATTATGATTGTTAACCAGCT+TGG + chr1_3:40016769-40016788 Msa0117930:CDS 30.0%
GATTAAATTAAGCACTAGCT+AGG - chr1_3:40016861-40016880 None:intergenic 30.0%
TAATCATTGATCAATCACAG+AGG + chr1_3:40016876-40016895 Msa0117930:intron 30.0%
ATCATTTGTAGTATAGCCTT+AGG - chr1_3:40016907-40016926 None:intergenic 30.0%
TAAGGCTATACTACAAATGA+TGG + chr1_3:40016906-40016925 Msa0117930:CDS 30.0%
AAGGCTATACTACAAATGAT+GGG + chr1_3:40016907-40016926 Msa0117930:CDS 30.0%
! ATGACTTTTGACATGAGAAA+TGG - chr1_3:40016947-40016966 None:intergenic 30.0%
!! AGTTTATTTTCATGAGACAG+TGG + chr1_3:40016976-40016995 Msa0117930:intron 30.0%
GTAATTTACTTCAACCTGTA+TGG + chr1_3:40017126-40017145 Msa0117930:intron 30.0%
CTCTATTTACTTTATCACAC+TGG + chr1_3:40017320-40017339 Msa0117930:intron 30.0%
TAGAGTAGGGAAAATTTGAA+AGG + chr1_3:40017389-40017408 Msa0117930:intron 30.0%
AAATAAGAAGGTAAAACTCG+TGG - chr1_3:40017483-40017502 None:intergenic 30.0%
AAAGGATTGAGTAGGAAATT+TGG - chr1_3:40017975-40017994 None:intergenic 30.0%
GTGATTCTTCTATTTGCATT+TGG - chr1_3:40018002-40018021 None:intergenic 30.0%
ACAAAAGAAAAGAACCAAAC+AGG + chr1_3:40018392-40018411 Msa0117930:CDS 30.0%
! TGAATCTGTTGTTATTGGCA+TGG - chr1_3:40015490-40015509 None:intergenic 35.0%
CAGCCTTGACAATACATAAA+AGG - chr1_3:40015513-40015532 None:intergenic 35.0%
! ATGATTTTTCCCCAGATCAT+AGG + chr1_3:40015691-40015710 Msa0117930:CDS 35.0%
! AATCACCTGAGTTTTCTGAA+AGG - chr1_3:40015730-40015749 None:intergenic 35.0%
AACTTCTACCACACAAGTTA+AGG - chr1_3:40015908-40015927 None:intergenic 35.0%
! AGCTCTGATGTTAGATATGT+CGG + chr1_3:40015984-40016003 Msa0117930:intron 35.0%
! CATATTGCTTTGGGTACAAA+TGG - chr1_3:40016217-40016236 None:intergenic 35.0%
! TTTAGAGCAATTTCAGCAGT+GGG - chr1_3:40016303-40016322 None:intergenic 35.0%
!!! TTTTAGAGCAATTTCAGCAG+TGG - chr1_3:40016304-40016323 None:intergenic 35.0%
AAACTATTCCAGCAATCGTT+CGG + chr1_3:40016321-40016340 Msa0117930:intron 35.0%
! GTGAGAAGAGTAGTTTTTGA+AGG + chr1_3:40016506-40016525 Msa0117930:intron 35.0%
ACAAACAATCTTCACCTTGT+GGG + chr1_3:40016653-40016672 Msa0117930:CDS 35.0%
!! TATACTACAAATGATGGGTG+TGG + chr1_3:40016912-40016931 Msa0117930:CDS 35.0%
TCATGTCAAAAGTCATTTGC+AGG + chr1_3:40016951-40016970 Msa0117930:CDS 35.0%
TCTACATTACACACGCATAA+AGG + chr1_3:40017095-40017114 Msa0117930:CDS 35.0%
GTAGGGAAAATTTGAAAGGA+AGG + chr1_3:40017393-40017412 Msa0117930:intron 35.0%
AAGGTTTGCAAAGTAGAGAA+GGG + chr1_3:40017412-40017431 Msa0117930:intron 35.0%
!! TGCATTTTAATATGCAGGGT+GGG - chr1_3:40017636-40017655 None:intergenic 35.0%
!! ATGCATTTTAATATGCAGGG+TGG - chr1_3:40017637-40017656 None:intergenic 35.0%
CTAATTGAACTATACTCACG+AGG + chr1_3:40017690-40017709 Msa0117930:intron 35.0%
AGGAAGGACAAATAACAGAT+GGG + chr1_3:40017814-40017833 Msa0117930:CDS 35.0%
CTATTTGCATTTGGCTGAAA+AGG - chr1_3:40017993-40018012 None:intergenic 35.0%
! GAAATATTATGTTGGCCTGA+TGG + chr1_3:40018165-40018184 Msa0117930:intron 35.0%
TACTGAAATATGAGACCTTG+AGG - chr1_3:40018221-40018240 None:intergenic 35.0%
ATTGCTGATATGTCCTCTAG+AGG - chr1_3:40015369-40015388 None:intergenic 40.0%
GTATGTCAAACTCATGCTGA+TGG - chr1_3:40015446-40015465 None:intergenic 40.0%
ACCAACAACCTGCTTCAAAT+TGG + chr1_3:40015572-40015591 Msa0117930:CDS 40.0%
CCAACAACCTGCTTCAAATT+GGG + chr1_3:40015573-40015592 Msa0117930:CDS 40.0%
TTCCCTCTTGTTCTGATAAG+AGG + chr1_3:40015658-40015677 Msa0117930:CDS 40.0%
TCCCTCTTGTTCTGATAAGA+GGG + chr1_3:40015659-40015678 Msa0117930:CDS 40.0%
ACCCTCTTATCAGAACAAGA+GGG - chr1_3:40015663-40015682 None:intergenic 40.0%
TTTCATGGGACCTATGATCT+GGG - chr1_3:40015704-40015723 None:intergenic 40.0%
ATTTCATGGGACCTATGATC+TGG - chr1_3:40015705-40015724 None:intergenic 40.0%
GGTATAGATGTATCCCAATG+CGG + chr1_3:40015849-40015868 Msa0117930:intron 40.0%
TTCATTCGATAAGGAGGTTG+AGG - chr1_3:40016075-40016094 None:intergenic 40.0%
! TTCTTTGTTTGTCGAAGTCG+CGG + chr1_3:40016107-40016126 Msa0117930:intron 40.0%
TAGCGGGAGTTAATTGTTGT+TGG + chr1_3:40016350-40016369 Msa0117930:intron 40.0%
GACAAACAATCTTCACCTTG+TGG + chr1_3:40016652-40016671 Msa0117930:CDS 40.0%
GTTGCAAAATCAAACCCACA+AGG - chr1_3:40016670-40016689 None:intergenic 40.0%
ATGATTGTTAACCAGCTTGG+TGG + chr1_3:40016772-40016791 Msa0117930:CDS 40.0%
ACACACGCATAAAGGTTTGA+AGG + chr1_3:40017103-40017122 Msa0117930:CDS 40.0%
CACACGCATAAAGGTTTGAA+GGG + chr1_3:40017104-40017123 Msa0117930:CDS 40.0%
GAAGGTTTGCAAAGTAGAGA+AGG + chr1_3:40017411-40017430 Msa0117930:intron 40.0%
AGGTTTGCAAAGTAGAGAAG+GGG + chr1_3:40017413-40017432 Msa0117930:intron 40.0%
!! CATTTTAATATGCAGGGTGG+GGG - chr1_3:40017634-40017653 None:intergenic 40.0%
!! GCATTTTAATATGCAGGGTG+GGG - chr1_3:40017635-40017654 None:intergenic 40.0%
GAGGAAGGACAAATAACAGA+TGG + chr1_3:40017813-40017832 Msa0117930:CDS 40.0%
TTGGCTGAAAAGGATTGAGT+AGG - chr1_3:40017983-40018002 None:intergenic 40.0%
ATCACGACAACTACAACTTG+AGG + chr1_3:40018017-40018036 Msa0117930:CDS 40.0%
AACTACAACTTGAGGTTGAG+AGG + chr1_3:40018025-40018044 Msa0117930:CDS 40.0%
ATCAACGACAATTCGAGGTA+CGG + chr1_3:40018052-40018071 Msa0117930:intron 40.0%
! TTATGTTGGCCTGATGGTAT+TGG + chr1_3:40018171-40018190 Msa0117930:intron 40.0%
TGAAATATGAGACCTTGAGG+AGG - chr1_3:40018218-40018237 None:intergenic 40.0%
!! ATCGGTCATTTTCTCCTGTT+TGG - chr1_3:40018409-40018428 None:intergenic 40.0%
!! GAGGACTAGCTATGCTACAA+GGG - chr1_3:40015350-40015369 None:intergenic 45.0%
!! AGAGGACTAGCTATGCTACA+AGG - chr1_3:40015351-40015370 None:intergenic 45.0%
TAGCATAGCTAGTCCTCTAG+AGG + chr1_3:40015353-40015372 Msa0117930:CDS 45.0%
! CCCAATTTGAAGCAGGTTGT+TGG - chr1_3:40015576-40015595 None:intergenic 45.0%
! TTCAGCACCCAATTTGAAGC+AGG - chr1_3:40015583-40015602 None:intergenic 45.0%
CACCCTCTTATCAGAACAAG+AGG - chr1_3:40015664-40015683 None:intergenic 45.0%
! TTGTTCTGATAAGAGGGTGC+TGG + chr1_3:40015665-40015684 Msa0117930:CDS 45.0%
TTCATGGGACCTATGATCTG+GGG - chr1_3:40015703-40015722 None:intergenic 45.0%
TAGAAAAGCCGAACGATTGC+TGG - chr1_3:40016332-40016351 None:intergenic 45.0%
! CAATCGTTCGGCTTTTCTAG+CGG + chr1_3:40016333-40016352 Msa0117930:intron 45.0%
!! AATCGTTCGGCTTTTCTAGC+GGG + chr1_3:40016334-40016353 Msa0117930:intron 45.0%
AATCACAGAGGCAAAGCCTA+AGG + chr1_3:40016888-40016907 Msa0117930:intron 45.0%
!! CATTGTTGCAGGAGTATCTG+AGG + chr1_3:40017794-40017813 Msa0117930:intron 45.0%
GAGCAATCAACGACAATTCG+AGG + chr1_3:40018047-40018066 Msa0117930:CDS 45.0%
GATGCAGCGAAATTTGCAAC+TGG + chr1_3:40018326-40018345 Msa0117930:intron 45.0%
!! TATTATATCATAAAAATAAA+TGG + chr1_3:40015958-40015977 Msa0117930:intron 5.0%
TTTGTTTGTCGAAGTCGCGG+TGG + chr1_3:40016110-40016129 Msa0117930:intron 50.0%
TTGTTTGTCGAAGTCGCGGT+GGG + chr1_3:40016111-40016130 Msa0117930:intron 50.0%
!! GTTGCAGGAGTATCTGAGGA+AGG + chr1_3:40017798-40017817 Msa0117930:intron 50.0%
!! TTGGCCTGATGGTATTGGCT+TGG + chr1_3:40018176-40018195 Msa0117930:intron 50.0%
GGTTCCAAGCCAATACCATC+AGG - chr1_3:40018183-40018202 None:intergenic 50.0%
TACTTTCAGGGCCACCAAGC+TGG - chr1_3:40016786-40016805 None:intergenic 55.0%
! TTGAGGAGGAGCACACACTC+AGG - chr1_3:40018204-40018223 None:intergenic 55.0%
TGAGTGTGTGCTCCTCCTCA+AGG + chr1_3:40018203-40018222 Msa0117930:intron 55.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 40015306 40018437 40015306 ID=Msa0117930;Name=Msa0117930
chr1_3 mRNA 40015306 40018437 40015306 ID=Msa0117930-mRNA-1;Parent=Msa0117930;Name=Msa0117930-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0|0|0|1|1|1|7|0|320
chr1_3 exon 40015306 40015743 40015306 ID=Msa0117930-mRNA-1:exon:12240;Parent=Msa0117930-mRNA-1
chr1_3 exon 40016631 40016802 40016631 ID=Msa0117930-mRNA-1:exon:12241;Parent=Msa0117930-mRNA-1
chr1_3 exon 40016896 40016972 40016896 ID=Msa0117930-mRNA-1:exon:12242;Parent=Msa0117930-mRNA-1
chr1_3 exon 40017086 40017125 40017086 ID=Msa0117930-mRNA-1:exon:12243;Parent=Msa0117930-mRNA-1
chr1_3 exon 40017805 40017859 40017805 ID=Msa0117930-mRNA-1:exon:12244;Parent=Msa0117930-mRNA-1
chr1_3 exon 40017999 40018068 40017999 ID=Msa0117930-mRNA-1:exon:12245;Parent=Msa0117930-mRNA-1
chr1_3 exon 40018327 40018437 40018327 ID=Msa0117930-mRNA-1:exon:12246;Parent=Msa0117930-mRNA-1
chr1_3 CDS 40015306 40015743 40015306 ID=Msa0117930-mRNA-1:cds;Parent=Msa0117930-mRNA-1
chr1_3 CDS 40016631 40016802 40016631 ID=Msa0117930-mRNA-1:cds;Parent=Msa0117930-mRNA-1
chr1_3 CDS 40016896 40016972 40016896 ID=Msa0117930-mRNA-1:cds;Parent=Msa0117930-mRNA-1
chr1_3 CDS 40017086 40017125 40017086 ID=Msa0117930-mRNA-1:cds;Parent=Msa0117930-mRNA-1
chr1_3 CDS 40017805 40017859 40017805 ID=Msa0117930-mRNA-1:cds;Parent=Msa0117930-mRNA-1
chr1_3 CDS 40017999 40018068 40017999 ID=Msa0117930-mRNA-1:cds;Parent=Msa0117930-mRNA-1
chr1_3 CDS 40018327 40018437 40018327 ID=Msa0117930-mRNA-1:cds;Parent=Msa0117930-mRNA-1
Gene Sequence

>Msa0117930

ATGTACATAAATCATTGGGATTATGAACATCAAAGCTATGACCCTTGTAGCATAGCTAGTCCTCTAGAGGACATATCAGCAATAATTCATGGATCAAATGTAAATGAAACCTATATGAATTTTCAAGCTCAACAACACCATCAGCATGAGTTTGACATACCTATTTGGAGCAATGAATTTACCATGCCAATAACAACAGATTCACCTTTTATGTATTGTCAAGGCTGTAAAGATAACTACTTAGAAAATATCACAAAACAAGATCAACCAACAACCTGCTTCAAATTGGGTGCTGAATCTTTAATGTCTAGCAGTGAAGATAGTATTTCTTCTTGTGAAAAGTGTAGTGAGTTTCCCTCTTGTTCTGATAAGAGGGTGCTGGAGAATGATTTTTCCCCAGATCATAGGTCCCATGAAATTTCCTTTCAGAAAACTCAGTGGGAATCATGTACAAAGCAAGACAAACAATCTTCACCTTGTGGGTTTGATTTTGCAACATCCAGCAACTCTGATTTTAAGATAACTGCAAAGGGTAAAAGAAGATTAAGATGGACAAAAGAATTGAATGAGTCATTTATTATGATTGTTAACCAGCTTGGTGGCCCTGAAAAGGCAAAGCCTAAGGCTATACTACAAATGATGGGTGTGGATGTGTTGACCATTTCTCATGTCAAAAGTCATTTGCAGAAATATCGTTCTACATTACACACGCATAAAGGTTTGAAGGGAGTATCTGAGGAAGGACAAATAACAGATGGGATTAATGAGCTTCAAGTTAAAATCCAAATGCAAATAGAAGAATCACGACAACTACAACTTGAGGTTGAGAGGAGCAATCAACGACAATTCGAGATGCAGCGAAATTTGCAACTGGTGATTGAACAGCAGAAGAAGCAGCTCAAGTTAATGTTAGATCAACAAAAGAAAAGAACCAAACAGGAGAAAATGACCGATTTAAGATGA
Protein sequence

>Msa0117930

MYINHWDYEHQSYDPCSIASPLEDISAIIHGSNVNETYMNFQAQQHHQHEFDIPIWSNEFTMPITTDSPFMYCQGCKDNYLENITKQDQPTTCFKLGAESLMSSSEDSISSCEKCSEFPSCSDKRVLENDFSPDHRSHEISFQKTQWESCTKQDKQSSPCGFDFATSSNSDFKITAKGKRRLRWTKELNESFIMIVNQLGGPEKAKPKAILQMMGVDVLTISHVKSHLQKYRSTLHTHKGLKGVSEEGQITDGINELQVKIQMQIEESRQLQLEVERSNQRQFEMQRNLQLVIEQQKKQLKLMLDQQKKRTKQEKMTDLR*