AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0119400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0119400 MtrunA17_Chr1g0177091 96.687 332 11 0 1 332 1 332 0.0 672
Msa0119400 MtrunA17_Chr7g0267581 70.606 330 88 6 1 325 1 326 3.61e-162 456
Msa0119400 MtrunA17_Chr8g0352251 50.400 125 49 4 128 241 109 231 1.25e-29 114
Msa0119400 MtrunA17_Chr2g0300261 49.624 133 49 4 116 231 14 145 1.71e-29 113
Msa0119400 MtrunA17_Chr2g0292411 49.533 107 52 1 133 237 80 186 3.20e-27 106
Msa0119400 MtrunA17_Chr3g0103391 50.000 116 44 4 127 231 125 237 5.18e-27 108
Msa0119400 MtrunA17_Chr8g0343451 47.414 116 46 4 120 222 110 223 5.66e-26 105
Msa0119400 MtrunA17_Chr2g0329811 36.723 177 100 4 95 260 108 283 5.83e-25 103
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0119400 AT5G06510.1 44.518 301 109 13 29 325 13 259 2.03e-61 197
Msa0119400 AT5G06510.5 44.518 301 109 13 29 325 13 259 2.03e-61 197
Msa0119400 AT5G06510.2 44.518 301 109 13 29 325 13 259 2.03e-61 197
Msa0119400 AT5G06510.6 44.518 301 109 13 29 325 13 259 2.03e-61 197
Msa0119400 AT3G05690.1 43.385 325 117 17 20 326 9 284 4.38e-56 184
Msa0119400 AT5G06510.3 48.511 235 76 9 93 325 19 210 1.16e-55 181
Msa0119400 AT3G05690.2 43.385 325 117 17 20 326 46 321 3.99e-55 183
Msa0119400 AT5G06510.4 51.269 197 58 8 131 325 4 164 8.15e-49 162
Msa0119400 AT5G06510.7 51.269 197 58 8 131 325 4 164 8.15e-49 162
Msa0119400 AT2G34720.1 40.462 173 86 5 97 256 19 187 1.47e-32 120
Msa0119400 AT1G30500.1 50.420 119 53 1 127 239 53 171 5.66e-32 118
Msa0119400 AT1G30500.2 48.438 128 56 1 122 239 48 175 3.02e-31 116
Msa0119400 AT2G34720.2 46.377 138 58 4 132 256 18 152 1.25e-30 114
Msa0119400 AT2G34720.3 46.377 138 58 4 132 256 18 152 1.25e-30 114
Msa0119400 AT1G72830.1 41.808 177 77 7 132 293 135 300 8.62e-30 116
Msa0119400 AT5G12840.4 34.843 287 147 13 1 266 1 268 1.24e-29 114
Msa0119400 AT5G12840.2 34.843 287 147 13 1 266 1 268 1.24e-29 114
Msa0119400 AT5G12840.5 46.053 152 62 6 127 266 126 269 6.15e-29 112
Msa0119400 AT5G12840.3 46.053 152 62 6 127 266 126 269 6.15e-29 112
Msa0119400 AT5G12840.1 46.053 152 62 6 127 266 126 269 6.15e-29 112
Msa0119400 AT1G72830.2 41.573 178 77 8 132 293 135 301 3.78e-28 112
Msa0119400 AT1G17590.3 59.259 81 31 2 143 223 153 231 6.73e-26 105
Msa0119400 AT1G17590.2 59.259 81 31 2 143 223 153 231 6.73e-26 105
Msa0119400 AT1G17590.4 59.259 81 31 2 143 223 153 231 6.73e-26 105
Msa0119400 AT1G17590.1 59.259 81 31 2 143 223 153 231 6.73e-26 105
Msa0119400 AT3G20910.1 47.059 136 49 6 133 254 126 252 5.25e-25 102
Msa0119400 AT1G54160.1 64.000 75 26 1 149 223 164 237 5.71e-25 102
Msa0119400 AT3G14020.2 51.961 102 40 3 132 229 140 236 1.32e-23 99.4
Msa0119400 AT3G14020.1 51.961 102 40 3 132 229 140 236 1.32e-23 99.4
Msa0119400 AT1G72830.3 37.952 166 74 6 132 293 135 275 2.18e-21 93.2

Find 76 sgRNAs with CRISPR-Local

Find 228 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGGATCTCAACCTGTTTA+TGG 0.217575 1_3:+43362523 Msa0119400:CDS
TACGGGCTCTTCTCGACTTA+TGG 0.224539 1_3:+43363133 Msa0119400:CDS
AACACAAAAGTTTCTGCTAA+TGG 0.270945 1_3:+43363971 Msa0119400:CDS
CGGATCTACTGTCCACTCTT+TGG 0.272014 1_3:+43364204 Msa0119400:CDS
GTTGAACAATTGTTTGATAA+AGG 0.289283 1_3:+43362637 Msa0119400:CDS
TTGGAAAGCTCTAGTGAAAA+AGG 0.317460 1_3:-43362571 None:intergenic
TGACGCGATTCGTGCATATA+TGG 0.329752 1_3:-43363902 None:intergenic
CTGTGCAAAATATCCTTATA+TGG 0.336858 1_3:+43363102 Msa0119400:CDS
GTATTGTCATGCCAACAAAA+TGG 0.358807 1_3:+43364251 Msa0119400:CDS
CCGTAAAATTGATCCATATA+AGG 0.372097 1_3:-43363115 None:intergenic
AACCAATTCACCATCTTTCC+AGG 0.379219 1_3:+43362667 Msa0119400:CDS
TCATGTTCTTTAAGATAAAC+AGG 0.380584 1_3:-43362433 None:intergenic
ACCTCTGACCCCGAGACGTT+TGG 0.381733 1_3:-43364130 None:intergenic
AGTTCTTACTCGGAGCAATT+TGG 0.382158 1_3:-43362603 None:intergenic
GGATCTACTGTCCACTCTTT+GGG 0.389114 1_3:+43364205 Msa0119400:CDS
AGCAATTTGGTCTATGTAGT+TGG 0.394015 1_3:-43362590 None:intergenic
TTGGGAGACATGATCGATAC+TGG 0.406979 1_3:+43364223 Msa0119400:CDS
TACCTGGAAAGATGGTGAAT+TGG 0.421188 1_3:-43362669 None:intergenic
TGCTTAGCATTCACGTATGT+TGG 0.430698 1_3:-43363694 None:intergenic
AGCTTTACTGTCAATCATAT+CGG 0.452099 1_3:+43364184 Msa0119400:CDS
CAAGACTCTCCTCCATAAAC+AGG 0.453464 1_3:-43362535 None:intergenic
GGAGGCAGTCCAAACGTCTC+GGG 0.456102 1_3:+43364121 Msa0119400:CDS
TTTATTCTGAAGCACAGCTT+TGG 0.466385 1_3:-43363752 None:intergenic
GATAAGGTTGCTTGATGATT+CGG 0.479300 1_3:-43362909 None:intergenic
TGTTAAGATTTACCATAGGC+TGG 0.484839 1_3:-43362986 None:intergenic
ACAAAATGGTTTGCAGCAGC+TGG 0.492247 1_3:+43364265 Msa0119400:CDS
AAACGACCACCGCATCCTCT+TGG 0.498137 1_3:-43363944 None:intergenic
AAAGTTTCTGCTAATGGAAA+CGG 0.509349 1_3:+43363977 Msa0119400:CDS
TGGAACTTTAAATTCGTCAA+AGG 0.512980 1_3:+43364090 Msa0119400:CDS
AGCAGCTGGTAGACAGCTGC+TGG 0.514662 1_3:+43364279 Msa0119400:CDS
CCTAGCTCAAAACGGTTAAG+TGG 0.514977 1_3:-43362957 None:intergenic
CCATGGTGGAGTAATGCCTA+TGG 0.516782 1_3:+43362505 Msa0119400:CDS
AATTCGTCAAAGGAGACAAA+CGG 0.518779 1_3:+43364100 Msa0119400:CDS
TTCGGAACTCTGAGAACCAC+TGG 0.518971 1_3:-43364050 None:intergenic
CCGAGTAAGAACTTAGTTCG+AGG 0.522823 1_3:+43362613 Msa0119400:CDS
CGGAGGCAGTCCAAACGTCT+CGG 0.528352 1_3:+43364120 Msa0119400:CDS
GGAAATGTCCACAATTCTGT+TGG 0.536104 1_3:+43362457 Msa0119400:CDS
GGTGACTAGCATGTATTCAC+AGG 0.537442 1_3:+43364150 Msa0119400:CDS
GACTTATGGACCTCAAATCT+CGG 0.538137 1_3:+43363147 Msa0119400:CDS
CCTCGAACTAAGTTCTTACT+CGG 0.539423 1_3:-43362613 None:intergenic
ATAACAAGTACCTGGAAAGA+TGG 0.540599 1_3:-43362677 None:intergenic
GGTTAAAACCTAGCTCAAAA+CGG 0.541551 1_3:-43362965 None:intergenic
GGCCTACAGCTGCAGTCCAG+TGG 0.549836 1_3:+43364034 Msa0119400:CDS
ATAAACAGGTTGAGATCCAT+AGG 0.553524 1_3:-43362521 None:intergenic
AACCTCAGATTGTAAGACTT+CGG 0.557052 1_3:-43364068 None:intergenic
TTCCGAAGTCTTACAATCTG+AGG 0.557924 1_3:+43364066 Msa0119400:CDS
AAATGATGATTGCAGTGATA+AGG 0.560313 1_3:-43362925 None:intergenic
TCCAAACGTCTCGGGGTCAG+AGG 0.561807 1_3:+43364129 Msa0119400:CDS
AAAACTGTCCAACAGAATTG+TGG 0.562402 1_3:-43362465 None:intergenic
GTTTATCTTAAAGAACATGA+AGG 0.564171 1_3:+43362436 Msa0119400:CDS
TCGTCAGATGTCATGCTAAG+TGG 0.564515 1_3:-43363667 None:intergenic
CTAATGGAAACGGTAAAAGC+AGG 0.568627 1_3:+43363987 Msa0119400:CDS
TAAAGACAAACCGAGATTTG+AGG 0.581306 1_3:-43363157 None:intergenic
GAAGTCTTACAATCTGAGGT+TGG 0.582591 1_3:+43364070 Msa0119400:CDS
ATGCGTAGACCAAGAGGATG+CGG 0.604666 1_3:+43363935 Msa0119400:CDS
CGATCATGTCTCCCAAAGAG+TGG 0.606030 1_3:-43364216 None:intergenic
GTTTATGGAGGAGAGTCTTG+TGG 0.624514 1_3:+43362538 Msa0119400:CDS
GAGGCAGTCCAAACGTCTCG+GGG 0.630984 1_3:+43364122 Msa0119400:CDS
TAAATTGATAAAGCGTAACA+AGG 0.648504 1_3:+43363771 Msa0119400:CDS
GGATCTCAACCTGTTTATGG+AGG 0.650456 1_3:+43362526 Msa0119400:CDS
GACATGATCGATACTGGACA+CGG 0.660565 1_3:+43364229 Msa0119400:CDS
CATGCAATGCGTAGACCAAG+AGG 0.668981 1_3:+43363929 Msa0119400:CDS
CGTAGACCAAGAGGATGCGG+TGG 0.670871 1_3:+43363938 Msa0119400:CDS
CATCTGTGACTACAGCACCA+TGG 0.674450 1_3:+43362488 Msa0119400:CDS
GTGAATGCTAAGCAATACCA+TGG 0.675463 1_3:+43363703 Msa0119400:CDS
GTGACTAGCATGTATTCACA+GGG 0.683616 1_3:+43364151 Msa0119400:CDS
AACCACTGGACTGCAGCTGT+AGG 0.688049 1_3:-43364036 None:intergenic
ACCATGGTATCATCAGACGC+AGG 0.688525 1_3:+43363719 Msa0119400:CDS
AGTGAAGAGAACATAAACAC+TGG 0.692663 1_3:+43364010 Msa0119400:CDS
GCCTGCGTCTGATGATACCA+TGG 0.693395 1_3:-43363720 None:intergenic
CCATAGGCATTACTCCACCA+TGG 0.694421 1_3:-43362505 None:intergenic
GAAGAGAACATAAACACTGG+TGG 0.703232 1_3:+43364013 Msa0119400:CDS
CTGTGACTACAGCACCATGG+TGG 0.710455 1_3:+43362491 Msa0119400:CDS
CTTAGCATGACATCTGACGA+CGG 0.732604 1_3:+43363670 Msa0119400:CDS
ACTAGCATGTATTCACAGGG+AGG 0.747620 1_3:+43364154 Msa0119400:CDS
TCGTCAAAGGAGACAAACGG+AGG 0.770276 1_3:+43364103 Msa0119400:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAATTATGGTTA+AGG + chr1_3:43363413-43363432 Msa0119400:intron 10.0%
!!! ATCATAATAATTCAATTTTA+AGG - chr1_3:43363513-43363532 None:intergenic 10.0%
!! GTATCTATATTTAATAAATT+AGG + chr1_3:43363630-43363649 Msa0119400:intron 10.0%
!! AAAAAAAAAAATTATGGTTA+AGG + chr1_3:43363413-43363432 Msa0119400:intron 10.0%
!!! ATCATAATAATTCAATTTTA+AGG - chr1_3:43363513-43363532 None:intergenic 10.0%
!! GTATCTATATTTAATAAATT+AGG + chr1_3:43363630-43363649 Msa0119400:intron 10.0%
!! AAAATCAATTCATTCAAAAC+CGG - chr1_3:43362714-43362733 None:intergenic 20.0%
!! TATGTAACATGATTTATGTT+CGG + chr1_3:43362769-43362788 Msa0119400:intron 20.0%
!! AATACTAAAACATGATCAAA+GGG - chr1_3:43363019-43363038 None:intergenic 20.0%
!! AAATACTAAAACATGATCAA+AGG - chr1_3:43363020-43363039 None:intergenic 20.0%
!! TGAATTTATGTACTAAAACA+CGG - chr1_3:43363051-43363070 None:intergenic 20.0%
!!! ATTTGTTGTTAATGAGTTTT+AGG + chr1_3:43363356-43363375 Msa0119400:intron 20.0%
!! GATTTAAATGAGAAGAAAAT+TGG + chr1_3:43363469-43363488 Msa0119400:intron 20.0%
!! CCTTAATACATGTATAAAAA+GGG - chr1_3:43363863-43363882 None:intergenic 20.0%
!! ACCTTAATACATGTATAAAA+AGG - chr1_3:43363864-43363883 None:intergenic 20.0%
!! AAAATCAATTCATTCAAAAC+CGG - chr1_3:43362714-43362733 None:intergenic 20.0%
!! TATGTAACATGATTTATGTT+CGG + chr1_3:43362769-43362788 Msa0119400:intron 20.0%
!! AATACTAAAACATGATCAAA+GGG - chr1_3:43363019-43363038 None:intergenic 20.0%
!! AAATACTAAAACATGATCAA+AGG - chr1_3:43363020-43363039 None:intergenic 20.0%
!! TGAATTTATGTACTAAAACA+CGG - chr1_3:43363051-43363070 None:intergenic 20.0%
!!! ATTTGTTGTTAATGAGTTTT+AGG + chr1_3:43363356-43363375 Msa0119400:intron 20.0%
!! GATTTAAATGAGAAGAAAAT+TGG + chr1_3:43363469-43363488 Msa0119400:intron 20.0%
!! CCTTAATACATGTATAAAAA+GGG - chr1_3:43363863-43363882 None:intergenic 20.0%
!! ACCTTAATACATGTATAAAA+AGG - chr1_3:43363864-43363883 None:intergenic 20.0%
!!! TCATGTTCTTTAAGATAAAC+AGG - chr1_3:43362436-43362455 None:intergenic 25.0%
! GTTTATCTTAAAGAACATGA+AGG + chr1_3:43362436-43362455 Msa0119400:CDS 25.0%
! GTTGAACAATTGTTTGATAA+AGG + chr1_3:43362637-43362656 Msa0119400:CDS 25.0%
!!! GATTTTTGTTAAGTTGATTC+TGG + chr1_3:43362728-43362747 Msa0119400:intron 25.0%
! TGTAGAAGAAAATGTTTGAA+TGG - chr1_3:43362862-43362881 None:intergenic 25.0%
!!! GTTTTGTTAAGATTTACCAT+AGG - chr1_3:43362993-43363012 None:intergenic 25.0%
!! CCTTATATGGATCAATTTTA+CGG + chr1_3:43363115-43363134 Msa0119400:CDS 25.0%
!! CTTATATGGATCAATTTTAC+GGG + chr1_3:43363116-43363135 Msa0119400:CDS 25.0%
! TAAATTGATAAAGCGTAACA+AGG + chr1_3:43363771-43363790 Msa0119400:CDS 25.0%
!! CCCTTTTTATACATGTATTA+AGG + chr1_3:43363860-43363879 Msa0119400:intron 25.0%
!!! TCATGTTCTTTAAGATAAAC+AGG - chr1_3:43362436-43362455 None:intergenic 25.0%
! GTTTATCTTAAAGAACATGA+AGG + chr1_3:43362436-43362455 Msa0119400:CDS 25.0%
! GTTGAACAATTGTTTGATAA+AGG + chr1_3:43362637-43362656 Msa0119400:CDS 25.0%
!!! GATTTTTGTTAAGTTGATTC+TGG + chr1_3:43362728-43362747 Msa0119400:intron 25.0%
! TGTAGAAGAAAATGTTTGAA+TGG - chr1_3:43362862-43362881 None:intergenic 25.0%
!!! GTTTTGTTAAGATTTACCAT+AGG - chr1_3:43362993-43363012 None:intergenic 25.0%
!! CCTTATATGGATCAATTTTA+CGG + chr1_3:43363115-43363134 Msa0119400:CDS 25.0%
!! CTTATATGGATCAATTTTAC+GGG + chr1_3:43363116-43363135 Msa0119400:CDS 25.0%
! TAAATTGATAAAGCGTAACA+AGG + chr1_3:43363771-43363790 Msa0119400:CDS 25.0%
!! CCCTTTTTATACATGTATTA+AGG + chr1_3:43363860-43363879 Msa0119400:intron 25.0%
CTAGTGAAAAAGGTTTCATT+TGG - chr1_3:43362564-43362583 None:intergenic 30.0%
TATATCGAATAACAAGTACC+TGG - chr1_3:43362688-43362707 None:intergenic 30.0%
!! CTTGTTATTCGATATAACTC+CGG + chr1_3:43362692-43362711 Msa0119400:intron 30.0%
AAATGATGATTGCAGTGATA+AGG - chr1_3:43362928-43362947 None:intergenic 30.0%
TTATGTACTAAAACACGGAT+TGG - chr1_3:43363046-43363065 None:intergenic 30.0%
CTGTGCAAAATATCCTTATA+TGG + chr1_3:43363102-43363121 Msa0119400:CDS 30.0%
CCGTAAAATTGATCCATATA+AGG - chr1_3:43363118-43363137 None:intergenic 30.0%
GATAGTTGTAAACAGATTTC+TGG - chr1_3:43363287-43363306 None:intergenic 30.0%
! AGTTTTCAAACATTGTTGTG+AGG - chr1_3:43363816-43363835 None:intergenic 30.0%
AACACAAAAGTTTCTGCTAA+TGG + chr1_3:43363971-43363990 Msa0119400:CDS 30.0%
AAAGTTTCTGCTAATGGAAA+CGG + chr1_3:43363977-43363996 Msa0119400:CDS 30.0%
TGGAACTTTAAATTCGTCAA+AGG + chr1_3:43364090-43364109 Msa0119400:CDS 30.0%
AGCTTTACTGTCAATCATAT+CGG + chr1_3:43364184-43364203 Msa0119400:CDS 30.0%
CTAGTGAAAAAGGTTTCATT+TGG - chr1_3:43362564-43362583 None:intergenic 30.0%
TATATCGAATAACAAGTACC+TGG - chr1_3:43362688-43362707 None:intergenic 30.0%
!! CTTGTTATTCGATATAACTC+CGG + chr1_3:43362692-43362711 Msa0119400:intron 30.0%
AAATGATGATTGCAGTGATA+AGG - chr1_3:43362928-43362947 None:intergenic 30.0%
TTATGTACTAAAACACGGAT+TGG - chr1_3:43363046-43363065 None:intergenic 30.0%
CTGTGCAAAATATCCTTATA+TGG + chr1_3:43363102-43363121 Msa0119400:CDS 30.0%
CCGTAAAATTGATCCATATA+AGG - chr1_3:43363118-43363137 None:intergenic 30.0%
GATAGTTGTAAACAGATTTC+TGG - chr1_3:43363287-43363306 None:intergenic 30.0%
! AGTTTTCAAACATTGTTGTG+AGG - chr1_3:43363816-43363835 None:intergenic 30.0%
AACACAAAAGTTTCTGCTAA+TGG + chr1_3:43363971-43363990 Msa0119400:CDS 30.0%
AAAGTTTCTGCTAATGGAAA+CGG + chr1_3:43363977-43363996 Msa0119400:CDS 30.0%
TGGAACTTTAAATTCGTCAA+AGG + chr1_3:43364090-43364109 Msa0119400:CDS 30.0%
AGCTTTACTGTCAATCATAT+CGG + chr1_3:43364184-43364203 Msa0119400:CDS 30.0%
AAAACTGTCCAACAGAATTG+TGG - chr1_3:43362468-43362487 None:intergenic 35.0%
! ATAAACAGGTTGAGATCCAT+AGG - chr1_3:43362524-43362543 None:intergenic 35.0%
TATGGATCTCAACCTGTTTA+TGG + chr1_3:43362523-43362542 Msa0119400:CDS 35.0%
! TTGGAAAGCTCTAGTGAAAA+AGG - chr1_3:43362574-43362593 None:intergenic 35.0%
AGCAATTTGGTCTATGTAGT+TGG - chr1_3:43362593-43362612 None:intergenic 35.0%
ATAACAAGTACCTGGAAAGA+TGG - chr1_3:43362680-43362699 None:intergenic 35.0%
! AAGTTGATTCTGGCTAAACA+TGG + chr1_3:43362738-43362757 Msa0119400:intron 35.0%
AAGAAAATGTTTGAATGGCC+AGG - chr1_3:43362857-43362876 None:intergenic 35.0%
! GATAAGGTTGCTTGATGATT+CGG - chr1_3:43362912-43362931 None:intergenic 35.0%
GGTTAAAACCTAGCTCAAAA+CGG - chr1_3:43362968-43362987 None:intergenic 35.0%
TGTTAAGATTTACCATAGGC+TGG - chr1_3:43362989-43363008 None:intergenic 35.0%
! TATTTTGCACAGATCTAGCA+TGG - chr1_3:43363094-43363113 None:intergenic 35.0%
TAAAGACAAACCGAGATTTG+AGG - chr1_3:43363160-43363179 None:intergenic 35.0%
AATGATTAAGAAAAGAGCCG+AGG - chr1_3:43363183-43363202 None:intergenic 35.0%
ATCTGCAGCAGATAAACAAA+TGG - chr1_3:43363585-43363604 None:intergenic 35.0%
!! TTTATTCTGAAGCACAGCTT+TGG - chr1_3:43363755-43363774 None:intergenic 35.0%
AGTGAAGAGAACATAAACAC+TGG + chr1_3:43364010-43364029 Msa0119400:CDS 35.0%
AACCTCAGATTGTAAGACTT+CGG - chr1_3:43364071-43364090 None:intergenic 35.0%
AATTCGTCAAAGGAGACAAA+CGG + chr1_3:43364100-43364119 Msa0119400:CDS 35.0%
GTATTGTCATGCCAACAAAA+TGG + chr1_3:43364251-43364270 Msa0119400:CDS 35.0%
AAAACTGTCCAACAGAATTG+TGG - chr1_3:43362468-43362487 None:intergenic 35.0%
! ATAAACAGGTTGAGATCCAT+AGG - chr1_3:43362524-43362543 None:intergenic 35.0%
TATGGATCTCAACCTGTTTA+TGG + chr1_3:43362523-43362542 Msa0119400:CDS 35.0%
! TTGGAAAGCTCTAGTGAAAA+AGG - chr1_3:43362574-43362593 None:intergenic 35.0%
AGCAATTTGGTCTATGTAGT+TGG - chr1_3:43362593-43362612 None:intergenic 35.0%
ATAACAAGTACCTGGAAAGA+TGG - chr1_3:43362680-43362699 None:intergenic 35.0%
! AAGTTGATTCTGGCTAAACA+TGG + chr1_3:43362738-43362757 Msa0119400:intron 35.0%
AAGAAAATGTTTGAATGGCC+AGG - chr1_3:43362857-43362876 None:intergenic 35.0%
! GATAAGGTTGCTTGATGATT+CGG - chr1_3:43362912-43362931 None:intergenic 35.0%
GGTTAAAACCTAGCTCAAAA+CGG - chr1_3:43362968-43362987 None:intergenic 35.0%
TGTTAAGATTTACCATAGGC+TGG - chr1_3:43362989-43363008 None:intergenic 35.0%
! TATTTTGCACAGATCTAGCA+TGG - chr1_3:43363094-43363113 None:intergenic 35.0%
TAAAGACAAACCGAGATTTG+AGG - chr1_3:43363160-43363179 None:intergenic 35.0%
AATGATTAAGAAAAGAGCCG+AGG - chr1_3:43363183-43363202 None:intergenic 35.0%
ATCTGCAGCAGATAAACAAA+TGG - chr1_3:43363585-43363604 None:intergenic 35.0%
!! TTTATTCTGAAGCACAGCTT+TGG - chr1_3:43363755-43363774 None:intergenic 35.0%
AGTGAAGAGAACATAAACAC+TGG + chr1_3:43364010-43364029 Msa0119400:CDS 35.0%
AACCTCAGATTGTAAGACTT+CGG - chr1_3:43364071-43364090 None:intergenic 35.0%
AATTCGTCAAAGGAGACAAA+CGG + chr1_3:43364100-43364119 Msa0119400:CDS 35.0%
GTATTGTCATGCCAACAAAA+TGG + chr1_3:43364251-43364270 Msa0119400:CDS 35.0%
GGAAATGTCCACAATTCTGT+TGG + chr1_3:43362457-43362476 Msa0119400:CDS 40.0%
AGTTCTTACTCGGAGCAATT+TGG - chr1_3:43362606-43362625 None:intergenic 40.0%
CCTCGAACTAAGTTCTTACT+CGG - chr1_3:43362616-43362635 None:intergenic 40.0%
AACCAATTCACCATCTTTCC+AGG + chr1_3:43362667-43362686 Msa0119400:CDS 40.0%
TACCTGGAAAGATGGTGAAT+TGG - chr1_3:43362672-43362691 None:intergenic 40.0%
! CTTTTGAACTTTCCTATGCC+TGG + chr1_3:43362836-43362855 Msa0119400:intron 40.0%
GACTTATGGACCTCAAATCT+CGG + chr1_3:43363147-43363166 Msa0119400:CDS 40.0%
! ATCTCGGTTTGTCTTTACCT+CGG + chr1_3:43363163-43363182 Msa0119400:intron 40.0%
TGCTTAGCATTCACGTATGT+TGG - chr1_3:43363697-43363716 None:intergenic 40.0%
GTGAATGCTAAGCAATACCA+TGG + chr1_3:43363703-43363722 Msa0119400:CDS 40.0%
CTAATGGAAACGGTAAAAGC+AGG + chr1_3:43363987-43364006 Msa0119400:CDS 40.0%
GAAGAGAACATAAACACTGG+TGG + chr1_3:43364013-43364032 Msa0119400:CDS 40.0%
TTCCGAAGTCTTACAATCTG+AGG + chr1_3:43364066-43364085 Msa0119400:CDS 40.0%
! GAAGTCTTACAATCTGAGGT+TGG + chr1_3:43364070-43364089 Msa0119400:CDS 40.0%
GTGACTAGCATGTATTCACA+GGG + chr1_3:43364151-43364170 Msa0119400:CDS 40.0%
GGAAATGTCCACAATTCTGT+TGG + chr1_3:43362457-43362476 Msa0119400:CDS 40.0%
AGTTCTTACTCGGAGCAATT+TGG - chr1_3:43362606-43362625 None:intergenic 40.0%
CCTCGAACTAAGTTCTTACT+CGG - chr1_3:43362616-43362635 None:intergenic 40.0%
AACCAATTCACCATCTTTCC+AGG + chr1_3:43362667-43362686 Msa0119400:CDS 40.0%
TACCTGGAAAGATGGTGAAT+TGG - chr1_3:43362672-43362691 None:intergenic 40.0%
! CTTTTGAACTTTCCTATGCC+TGG + chr1_3:43362836-43362855 Msa0119400:intron 40.0%
GACTTATGGACCTCAAATCT+CGG + chr1_3:43363147-43363166 Msa0119400:CDS 40.0%
! ATCTCGGTTTGTCTTTACCT+CGG + chr1_3:43363163-43363182 Msa0119400:intron 40.0%
TGCTTAGCATTCACGTATGT+TGG - chr1_3:43363697-43363716 None:intergenic 40.0%
GTGAATGCTAAGCAATACCA+TGG + chr1_3:43363703-43363722 Msa0119400:CDS 40.0%
CTAATGGAAACGGTAAAAGC+AGG + chr1_3:43363987-43364006 Msa0119400:CDS 40.0%
GAAGAGAACATAAACACTGG+TGG + chr1_3:43364013-43364032 Msa0119400:CDS 40.0%
TTCCGAAGTCTTACAATCTG+AGG + chr1_3:43364066-43364085 Msa0119400:CDS 40.0%
! GAAGTCTTACAATCTGAGGT+TGG + chr1_3:43364070-43364089 Msa0119400:CDS 40.0%
GTGACTAGCATGTATTCACA+GGG + chr1_3:43364151-43364170 Msa0119400:CDS 40.0%
GGATCTCAACCTGTTTATGG+AGG + chr1_3:43362526-43362545 Msa0119400:CDS 45.0%
CAAGACTCTCCTCCATAAAC+AGG - chr1_3:43362538-43362557 None:intergenic 45.0%
GTTTATGGAGGAGAGTCTTG+TGG + chr1_3:43362538-43362557 Msa0119400:CDS 45.0%
CCGAGTAAGAACTTAGTTCG+AGG + chr1_3:43362613-43362632 Msa0119400:CDS 45.0%
ATGTTTGAATGGCCAGGCAT+AGG - chr1_3:43362851-43362870 None:intergenic 45.0%
CCTAGCTCAAAACGGTTAAG+TGG - chr1_3:43362960-43362979 None:intergenic 45.0%
!! CCACTTAACCGTTTTGAGCT+AGG + chr1_3:43362957-43362976 Msa0119400:CDS 45.0%
!! GCTAGGTTTTAACCAGCCTA+TGG + chr1_3:43362974-43362993 Msa0119400:CDS 45.0%
TGCACAGATCTAGCATGGAA+AGG - chr1_3:43363089-43363108 None:intergenic 45.0%
TCGTCAGATGTCATGCTAAG+TGG - chr1_3:43363670-43363689 None:intergenic 45.0%
CTTAGCATGACATCTGACGA+CGG + chr1_3:43363670-43363689 Msa0119400:CDS 45.0%
TGACGCGATTCGTGCATATA+TGG - chr1_3:43363905-43363924 None:intergenic 45.0%
GGTGACTAGCATGTATTCAC+AGG + chr1_3:43364150-43364169 Msa0119400:CDS 45.0%
ACTAGCATGTATTCACAGGG+AGG + chr1_3:43364154-43364173 Msa0119400:CDS 45.0%
GGATCTACTGTCCACTCTTT+GGG + chr1_3:43364205-43364224 Msa0119400:CDS 45.0%
TTGGGAGACATGATCGATAC+TGG + chr1_3:43364223-43364242 Msa0119400:CDS 45.0%
GACATGATCGATACTGGACA+CGG + chr1_3:43364229-43364248 Msa0119400:CDS 45.0%
! GCTGCTGCAAACCATTTTGT+TGG - chr1_3:43364265-43364284 None:intergenic 45.0%
! ACAAAATGGTTTGCAGCAGC+TGG + chr1_3:43364265-43364284 Msa0119400:CDS 45.0%
GGATCTCAACCTGTTTATGG+AGG + chr1_3:43362526-43362545 Msa0119400:CDS 45.0%
CAAGACTCTCCTCCATAAAC+AGG - chr1_3:43362538-43362557 None:intergenic 45.0%
GTTTATGGAGGAGAGTCTTG+TGG + chr1_3:43362538-43362557 Msa0119400:CDS 45.0%
CCGAGTAAGAACTTAGTTCG+AGG + chr1_3:43362613-43362632 Msa0119400:CDS 45.0%
ATGTTTGAATGGCCAGGCAT+AGG - chr1_3:43362851-43362870 None:intergenic 45.0%
CCTAGCTCAAAACGGTTAAG+TGG - chr1_3:43362960-43362979 None:intergenic 45.0%
!! CCACTTAACCGTTTTGAGCT+AGG + chr1_3:43362957-43362976 Msa0119400:CDS 45.0%
!! GCTAGGTTTTAACCAGCCTA+TGG + chr1_3:43362974-43362993 Msa0119400:CDS 45.0%
TGCACAGATCTAGCATGGAA+AGG - chr1_3:43363089-43363108 None:intergenic 45.0%
TCGTCAGATGTCATGCTAAG+TGG - chr1_3:43363670-43363689 None:intergenic 45.0%
CTTAGCATGACATCTGACGA+CGG + chr1_3:43363670-43363689 Msa0119400:CDS 45.0%
TGACGCGATTCGTGCATATA+TGG - chr1_3:43363905-43363924 None:intergenic 45.0%
GGTGACTAGCATGTATTCAC+AGG + chr1_3:43364150-43364169 Msa0119400:CDS 45.0%
ACTAGCATGTATTCACAGGG+AGG + chr1_3:43364154-43364173 Msa0119400:CDS 45.0%
GGATCTACTGTCCACTCTTT+GGG + chr1_3:43364205-43364224 Msa0119400:CDS 45.0%
TTGGGAGACATGATCGATAC+TGG + chr1_3:43364223-43364242 Msa0119400:CDS 45.0%
GACATGATCGATACTGGACA+CGG + chr1_3:43364229-43364248 Msa0119400:CDS 45.0%
! GCTGCTGCAAACCATTTTGT+TGG - chr1_3:43364265-43364284 None:intergenic 45.0%
! ACAAAATGGTTTGCAGCAGC+TGG + chr1_3:43364265-43364284 Msa0119400:CDS 45.0%
!! TGAAATAAAAAAAAAAATTA+TGG + chr1_3:43363407-43363426 Msa0119400:intron 5.0%
!! TATCTATATTTAATAAATTA+GGG + chr1_3:43363631-43363650 Msa0119400:intron 5.0%
!! TGAAATAAAAAAAAAAATTA+TGG + chr1_3:43363407-43363426 Msa0119400:intron 5.0%
!! TATCTATATTTAATAAATTA+GGG + chr1_3:43363631-43363650 Msa0119400:intron 5.0%
CATCTGTGACTACAGCACCA+TGG + chr1_3:43362488-43362507 Msa0119400:CDS 50.0%
CCATAGGCATTACTCCACCA+TGG - chr1_3:43362508-43362527 None:intergenic 50.0%
! CCATGGTGGAGTAATGCCTA+TGG + chr1_3:43362505-43362524 Msa0119400:CDS 50.0%
TACGGGCTCTTCTCGACTTA+TGG + chr1_3:43363133-43363152 Msa0119400:CDS 50.0%
ACCATGGTATCATCAGACGC+AGG + chr1_3:43363719-43363738 Msa0119400:CDS 50.0%
CATGCAATGCGTAGACCAAG+AGG + chr1_3:43363929-43363948 Msa0119400:CDS 50.0%
ATGCGTAGACCAAGAGGATG+CGG + chr1_3:43363935-43363954 Msa0119400:CDS 50.0%
! TTCGGAACTCTGAGAACCAC+TGG - chr1_3:43364053-43364072 None:intergenic 50.0%
TCGTCAAAGGAGACAAACGG+AGG + chr1_3:43364103-43364122 Msa0119400:CDS 50.0%
CGGATCTACTGTCCACTCTT+TGG + chr1_3:43364204-43364223 Msa0119400:CDS 50.0%
CGATCATGTCTCCCAAAGAG+TGG - chr1_3:43364219-43364238 None:intergenic 50.0%
CATCTGTGACTACAGCACCA+TGG + chr1_3:43362488-43362507 Msa0119400:CDS 50.0%
CCATAGGCATTACTCCACCA+TGG - chr1_3:43362508-43362527 None:intergenic 50.0%
! CCATGGTGGAGTAATGCCTA+TGG + chr1_3:43362505-43362524 Msa0119400:CDS 50.0%
TACGGGCTCTTCTCGACTTA+TGG + chr1_3:43363133-43363152 Msa0119400:CDS 50.0%
ACCATGGTATCATCAGACGC+AGG + chr1_3:43363719-43363738 Msa0119400:CDS 50.0%
CATGCAATGCGTAGACCAAG+AGG + chr1_3:43363929-43363948 Msa0119400:CDS 50.0%
ATGCGTAGACCAAGAGGATG+CGG + chr1_3:43363935-43363954 Msa0119400:CDS 50.0%
! TTCGGAACTCTGAGAACCAC+TGG - chr1_3:43364053-43364072 None:intergenic 50.0%
TCGTCAAAGGAGACAAACGG+AGG + chr1_3:43364103-43364122 Msa0119400:CDS 50.0%
CGGATCTACTGTCCACTCTT+TGG + chr1_3:43364204-43364223 Msa0119400:CDS 50.0%
CGATCATGTCTCCCAAAGAG+TGG - chr1_3:43364219-43364238 None:intergenic 50.0%
CTGTGACTACAGCACCATGG+TGG + chr1_3:43362491-43362510 Msa0119400:CDS 55.0%
GCCTGCGTCTGATGATACCA+TGG - chr1_3:43363723-43363742 None:intergenic 55.0%
AAACGACCACCGCATCCTCT+TGG - chr1_3:43363947-43363966 None:intergenic 55.0%
AACCACTGGACTGCAGCTGT+AGG - chr1_3:43364039-43364058 None:intergenic 55.0%
CTGTGACTACAGCACCATGG+TGG + chr1_3:43362491-43362510 Msa0119400:CDS 55.0%
GCCTGCGTCTGATGATACCA+TGG - chr1_3:43363723-43363742 None:intergenic 55.0%
AAACGACCACCGCATCCTCT+TGG - chr1_3:43363947-43363966 None:intergenic 55.0%
AACCACTGGACTGCAGCTGT+AGG - chr1_3:43364039-43364058 None:intergenic 55.0%
CGTAGACCAAGAGGATGCGG+TGG + chr1_3:43363938-43363957 Msa0119400:CDS 60.0%
CGGAGGCAGTCCAAACGTCT+CGG + chr1_3:43364120-43364139 Msa0119400:CDS 60.0%
GGAGGCAGTCCAAACGTCTC+GGG + chr1_3:43364121-43364140 Msa0119400:CDS 60.0%
GAGGCAGTCCAAACGTCTCG+GGG + chr1_3:43364122-43364141 Msa0119400:CDS 60.0%
TCCAAACGTCTCGGGGTCAG+AGG + chr1_3:43364129-43364148 Msa0119400:CDS 60.0%
ACCTCTGACCCCGAGACGTT+TGG - chr1_3:43364133-43364152 None:intergenic 60.0%
AGCAGCTGGTAGACAGCTGC+TGG + chr1_3:43364279-43364298 Msa0119400:CDS 60.0%
CGTAGACCAAGAGGATGCGG+TGG + chr1_3:43363938-43363957 Msa0119400:CDS 60.0%
CGGAGGCAGTCCAAACGTCT+CGG + chr1_3:43364120-43364139 Msa0119400:CDS 60.0%
GGAGGCAGTCCAAACGTCTC+GGG + chr1_3:43364121-43364140 Msa0119400:CDS 60.0%
GAGGCAGTCCAAACGTCTCG+GGG + chr1_3:43364122-43364141 Msa0119400:CDS 60.0%
TCCAAACGTCTCGGGGTCAG+AGG + chr1_3:43364129-43364148 Msa0119400:CDS 60.0%
ACCTCTGACCCCGAGACGTT+TGG - chr1_3:43364133-43364152 None:intergenic 60.0%
AGCAGCTGGTAGACAGCTGC+TGG + chr1_3:43364279-43364298 Msa0119400:CDS 60.0%
GGCCTACAGCTGCAGTCCAG+TGG + chr1_3:43364034-43364053 Msa0119400:CDS 65.0%
GGCCTACAGCTGCAGTCCAG+TGG + chr1_3:43364034-43364053 Msa0119400:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 43362421 43364309 43362421 ID=Msa0119400;Name=Msa0119400
chr1_3 mRNA 43362421 43364309 43362421 ID=Msa0119400-mRNA-1;Parent=Msa0119400;Name=Msa0119400-mRNA-1;_AED=0.05;_eAED=0.06;_QI=0|0|0|1|1|1|5|0|332
chr1_3 exon 43362421 43362688 43362421 ID=Msa0119400-mRNA-1:exon:12965;Parent=Msa0119400-mRNA-1
chr1_3 exon 43362883 43362995 43362883 ID=Msa0119400-mRNA-1:exon:12966;Parent=Msa0119400-mRNA-1
chr1_3 exon 43363100 43363168 43363100 ID=Msa0119400-mRNA-1:exon:12967;Parent=Msa0119400-mRNA-1
chr1_3 exon 43363652 43363792 43363652 ID=Msa0119400-mRNA-1:exon:12968;Parent=Msa0119400-mRNA-1
chr1_3 exon 43363902 43364309 43363902 ID=Msa0119400-mRNA-1:exon:12969;Parent=Msa0119400-mRNA-1
chr1_3 CDS 43362421 43362688 43362421 ID=Msa0119400-mRNA-1:cds;Parent=Msa0119400-mRNA-1
chr1_3 CDS 43362883 43362995 43362883 ID=Msa0119400-mRNA-1:cds;Parent=Msa0119400-mRNA-1
chr1_3 CDS 43363100 43363168 43363100 ID=Msa0119400-mRNA-1:cds;Parent=Msa0119400-mRNA-1
chr1_3 CDS 43363652 43363792 43363652 ID=Msa0119400-mRNA-1:cds;Parent=Msa0119400-mRNA-1
chr1_3 CDS 43363902 43364309 43363902 ID=Msa0119400-mRNA-1:cds;Parent=Msa0119400-mRNA-1
Gene Sequence

>Msa0119400

ATGGCTATGCAACCTGTTTATCTTAAAGAACATGAAGGAAATGTCCACAATTCTGTTGGACAGTTTTCATCTGTGACTACAGCACCATGGTGGAGTAATGCCTATGGATCTCAACCTGTTTATGGAGGAGAGTCTTGTGGCCAAATGAAACCTTTTTCACTAGAGCTTTCCAACTACATAGACCAAATTGCTCCGAGTAAGAACTTAGTTCGAGGAGTTGAACAATTGTTTGATAAAGGACATACAAACCAATTCACCATCTTTCCAGATGATTGTAAGATGTCAGTTGATACACCGAATCATCAAGCAACCTTATCACTGCAATCATCATTTGCTGCTGAGCCACTTAACCGTTTTGAGCTAGGTTTTAACCAGCCTATGATCTGTGCAAAATATCCTTATATGGATCAATTTTACGGGCTCTTCTCGACTTATGGACCTCAAATCTCGGGACGTGTTATGCTTCCACTTAGCATGACATCTGACGACGGACCAACATACGTGAATGCTAAGCAATACCATGGTATCATCAGACGCAGGCATTCTCGTGCCAAAGCTGTGCTTCAGAATAAATTGATAAAGCGTAACAAGCCATATATGCACGAATCGCGTCATCTACATGCAATGCGTAGACCAAGAGGATGCGGTGGTCGTTTCTTGAACACAAAAGTTTCTGCTAATGGAAACGGTAAAAGCAGGAGTGAAGAGAACATAAACACTGGTGGCCTACAGCTGCAGTCCAGTGGTTCTCAGAGTTCCGAAGTCTTACAATCTGAGGTTGGAACTTTAAATTCGTCAAAGGAGACAAACGGAGGCAGTCCAAACGTCTCGGGGTCAGAGGTGACTAGCATGTATTCACAGGGAGGTCTTGATAGCTTTACTGTCAATCATATCGGATCTACTGTCCACTCTTTGGGAGACATGATCGATACTGGACACGGTATTGTCATGCCAACAAAATGGTTTGCAGCAGCTGGTAGACAGCTGCTGGAACCATAA
Protein sequence

>Msa0119400

MAMQPVYLKEHEGNVHNSVGQFSSVTTAPWWSNAYGSQPVYGGESCGQMKPFSLELSNYIDQIAPSKNLVRGVEQLFDKGHTNQFTIFPDDCKMSVDTPNHQATLSLQSSFAAEPLNRFELGFNQPMICAKYPYMDQFYGLFSTYGPQISGRVMLPLSMTSDDGPTYVNAKQYHGIIRRRHSRAKAVLQNKLIKRNKPYMHESRHLHAMRRPRGCGGRFLNTKVSANGNGKSRSEENINTGGLQLQSSGSQSSEVLQSEVGTLNSSKETNGGSPNVSGSEVTSMYSQGGLDSFTVNHIGSTVHSLGDMIDTGHGIVMPTKWFAAAGRQLLEP*