AlfalfaGEDB | Msa0119640

M. sativa cultivar zhongmu-4 / Msa0119640

KEGG: -
GO: -
PFAM: PF09133.13

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0119640	sp\|F4KCE9\|KNL2_ARATH	37.931	116	59	4	76	179	11	125	1.55e-13	77.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0119640	A0A072VLC9	78.926	503	63	6	54	520	1	496	0.0	741

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TFs/TRs:

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Gene ID	Type	Classification
Msa0119640	TR	Others

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0119640	MtrunA17_Chr1g0182621	78.926	503	63	6	54	520	1	496	0.0	741
Msa0119640	MtrunA17_Chr1g0182611	49.136	405	156	8	54	413	1	400	3.75e-107	328
Msa0119640	MtrunA17_Chr1g0182611	57.414	263	85	2	285	520	131	393	7.62e-82	263

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0119640	AT5G02520.2	37.931	116	59	4	76	179	11	125	1.49e-14	77.0
Msa0119640	AT5G02520.1	37.931	116	59	4	76	179	11	125	1.57e-14	77.0
Msa0119640	AT5G02520.3	37.931	116	59	4	76	179	11	125	2.09e-14	76.3

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Find 157 sgRNAs with CRISPR-Local

Find 255 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAGTTGGGTTATCGTT+GGG	0.448632	1_3:+43774994	None:intergenic
AAAGGGGTTCTATACAATGG+CGG	0.709873	1_3:+43774806	None:intergenic
AAAGTCCATTCCTACTTCGT+CGG	0.613142	1_3:-43773717	Msa0119640:CDS
AAATACCGACGAAGTAGGAA+TGG	0.585417	1_3:+43773712	None:intergenic
AACCACTGTTTAGCCATCTC+CGG	0.530631	1_3:-43774392	Msa0119640:CDS
AAGACTTTACATTCATCTCC+GGG	0.513597	1_3:+43773653	None:intergenic
AAGCAGCATCCTGAGTGTAG+AGG	0.635067	1_3:-43773536	Msa0119640:CDS
AATATTGTTCATGGTAGTAG+CGG	0.636935	1_3:-43772715	Msa0119640:CDS
AATGGCGGATAGAGAAATAG+AGG	0.528768	1_3:+43774821	None:intergenic
AATTGCAAGACTCCATTCCC+CGG	0.573734	1_3:-43773671	Msa0119640:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATAGTTGGGTTATCGTT+GGG	+	chr1_3:43771644-43771663	None:intergenic	30.0%
AAGCCGTTAAAAAAAATTGC+AGG	+	chr1_3:43771877-43771896	None:intergenic	30.0%
ACAAAGATCAACATTGGTAA+AGG	-	chr1_3:43771983-43772002	Msa0119640:intron	30.0%
AAGAAGAAGAAGAAGTTGTA+GGG	+	chr1_3:43772146-43772165	None:intergenic	30.0%
GAACGGTTACATTATACTAT+TGG	-	chr1_3:43772193-43772212	Msa0119640:intron	30.0%
TGAATCAAGCATAAACTGAA+GGG	+	chr1_3:43772305-43772324	None:intergenic	30.0%
TTGAATCAAGCATAAACTGA+AGG	+	chr1_3:43772306-43772325	None:intergenic	30.0%
CACATTTAATGTTGTGATTC+AGG	-	chr1_3:43772351-43772370	Msa0119640:three_prime_UTR	30.0%
TAAGACTTTACATTCATCTC+CGG	+	chr1_3:43772986-43773005	None:intergenic	30.0%
GTAAAGTCTTAAAGATGAGT+AGG	-	chr1_3:43772995-43773014	Msa0119640:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	43771624	43775033	43771624	ID=Msa0119640;Name=Msa0119640
chr1_3	mRNA	43771624	43775033	43771624	ID=Msa0119640-mRNA-1;Parent=Msa0119640;Name=Msa0119640-mRNA-1;_AED=0.07;_eAED=0.07;_QI=100\|0.6\|0.45\|1\|0.6\|0.45\|11\|399\|534
chr1_3	exon	43771624	43771840	43771624	ID=Msa0119640-mRNA-1:exon:13121;Parent=Msa0119640-mRNA-1
chr1_3	exon	43772021	43772079	43772021	ID=Msa0119640-mRNA-1:exon:13120;Parent=Msa0119640-mRNA-1
chr1_3	exon	43772279	43772850	43772279	ID=Msa0119640-mRNA-1:exon:13119;Parent=Msa0119640-mRNA-1
chr1_3	exon	43772975	43773217	43772975	ID=Msa0119640-mRNA-1:exon:13118;Parent=Msa0119640-mRNA-1
chr1_3	exon	43773417	43773567	43773417	ID=Msa0119640-mRNA-1:exon:13117;Parent=Msa0119640-mRNA-1
chr1_3	exon	43773580	43773746	43773580	ID=Msa0119640-mRNA-1:exon:13116;Parent=Msa0119640-mRNA-1
chr1_3	exon	43773862	43774009	43773862	ID=Msa0119640-mRNA-1:exon:13115;Parent=Msa0119640-mRNA-1
chr1_3	exon	43774150	43774187	43774150	ID=Msa0119640-mRNA-1:exon:13114;Parent=Msa0119640-mRNA-1
chr1_3	exon	43774208	43774284	43774208	ID=Msa0119640-mRNA-1:exon:13113;Parent=Msa0119640-mRNA-1
chr1_3	exon	43774377	43774595	43774377	ID=Msa0119640-mRNA-1:exon:13112;Parent=Msa0119640-mRNA-1
chr1_3	exon	43774821	43775033	43774821	ID=Msa0119640-mRNA-1:exon:13111;Parent=Msa0119640-mRNA-1
chr1_3	five_prime_UTR	43774934	43775033	43774934	ID=Msa0119640-mRNA-1:five_prime_utr;Parent=Msa0119640-mRNA-1
chr1_3	CDS	43774821	43774933	43774821	ID=Msa0119640-mRNA-1:cds;Parent=Msa0119640-mRNA-1
chr1_3	CDS	43774377	43774595	43774377	ID=Msa0119640-mRNA-1:cds;Parent=Msa0119640-mRNA-1
chr1_3	CDS	43774208	43774284	43774208	ID=Msa0119640-mRNA-1:cds;Parent=Msa0119640-mRNA-1
chr1_3	CDS	43774150	43774187	43774150	ID=Msa0119640-mRNA-1:cds;Parent=Msa0119640-mRNA-1
chr1_3	CDS	43773862	43774009	43773862	ID=Msa0119640-mRNA-1:cds;Parent=Msa0119640-mRNA-1
chr1_3	CDS	43773580	43773746	43773580	ID=Msa0119640-mRNA-1:cds;Parent=Msa0119640-mRNA-1
chr1_3	CDS	43773417	43773567	43773417	ID=Msa0119640-mRNA-1:cds;Parent=Msa0119640-mRNA-1
chr1_3	CDS	43772975	43773217	43772975	ID=Msa0119640-mRNA-1:cds;Parent=Msa0119640-mRNA-1
chr1_3	CDS	43772402	43772850	43772402	ID=Msa0119640-mRNA-1:cds;Parent=Msa0119640-mRNA-1
chr1_3	three_prime_UTR	43772279	43772401	43772279	ID=Msa0119640-mRNA-1:three_prime_utr;Parent=Msa0119640-mRNA-1
chr1_3	three_prime_UTR	43772021	43772079	43772021	ID=Msa0119640-mRNA-1:three_prime_utr;Parent=Msa0119640-mRNA-1
chr1_3	three_prime_UTR	43771624	43771840	43771624	ID=Msa0119640-mRNA-1:three_prime_utr;Parent=Msa0119640-mRNA-1

Gene Sequence

>Msa0119640

ATGTTCTCTTCTCTCAAGTTCCAAAGTCGAAACCCATACGAATCCGCACCATCTGCCGGCAGCTGCGCCACCACCACACTCCATCGGTCTCCTCTATTTCTCTATCCGCCATTTGTGAGAGAGTCTCCTCTCATCTCACTTATCTCTCTTCAACCAAACATGGCTGACTTCACTCCTACTTCTTCTAATTGCACCCCTACAACTTCTTCTTCTTCTTCTTCTTCTTCTTCATCTTGCTTCCAACGAACGGTTACATTATACTATTGGTGGCTCGTTAAATCATCCCAAGGAAACAACCACTGTTTAGCCATCTCCGGCATCTCTTCCACAAAGGAAGAAGCGGTTCGGGTATTTAATTCCGCTCCTATTATCAAAAGATATGATGAGGTTTCCCTTGAGACTGTCGATGATAAACAACGCACGCTGGATAATGCTTTTACTCCTCAGATTTTCAAGAGTTTTTTATTTGGCTTTCCTGAAAACTGGGAAACCTCTGTTCTAGCTTGCTTTAGAGAAGAATCAAAAATCGGTACTGATTCGGGCAATGCTGTTTTGGATAATGTATCTGCATTTTATCAAGAAATTTTCTCTAACGGTGTGGAAAAGTCCATTCCTACTTCGTCGGTATTTCCTGAAGAGGCCCTAGAGAATTGCAAGACTCCATTCCCCGGAGATGAATGTAAAGTCTTAAAGATGAGTAGGGTTGATGTTGCTTGTGGTAGTGGTAAAAATAGACGTAGTACCAGGTTGCATAATATTAAAAAGAAGCAGAAGCAGCATCCTGAGTGTAGAGGTCCTTTCAAGCATCCAGATGGAGAGCCGAGCTCTACTTCAAAGGCCATGGAAAATCATGATTCGGACACTGTTGTTCCGGATAATGTACCAGCAAGTCTTCCAGAAATATCATTTGATGTTGAACAAGATATGTCAATTAAGATGAGTAAGGTCAATGATGTTCATGGTAGTGGCCGGACTAGACGTAGTGGCCGGTTGCATAATGCTAAGGACTGCCAGAAGAAGCAGCCTGCGTCTGGAGATCCTCTCAAGAATCCAGAGAAAGAGCAGAGCTCTACCTTGAAGGTTGTGGAAGATCATGATTTGGATACTGCTGTTCCTGATAATGTATCAACAAATCTTCTAGAAATATTATCTGAAGCTGTGGAGAAGTCTTTTCCTACTTCTTTGGTATCACCGGATAAGACCACGAGAGATCGCAACAAGGCACTTCTTGAAGATGAACGGGACATGTCAATTAAGACGTGTAGGGTCAATATTGTTCATGGTAGTAGCGGGAATAGACGTAGTGCCCGGATGCATAATGTTATACCCTACCAGAGGAAGCAACCTGCTACTGGAGATCCTGCAACACATCCAGATAAAGACCAGATCTCTGCCTTAGCAGCCTTGGAATTGAGTGATGGAGGACTGGAAAGTCTATCGACACCGGTTCAGTCAAAAAAGGGAAGAGTGAACACAATGTCTGGGCAGGTCACTAATAAATTGAGTTCCAGAAATTCCAAAACTTTTTCAGCAAAAACTGAAGGATGTTATAAGAAAAAAGGGCGATGGTTGAAACAGAAGCGGTCACACCTAAGAGAAAAATGA

Protein sequence

>Msa0119640

MFSSLKFQSRNPYESAPSAGSCATTTLHRSPLFLYPPFVRESPLISLISLQPNMADFTPTSSNCTPTTSSSSSSSSSSCFQRTVTLYYWWLVKSSQGNNHCLAISGISSTKEEAVRVFNSAPIIKRYDEVSLETVDDKQRTLDNAFTPQIFKSFLFGFPENWETSVLACFREESKIGTDSGNAVLDNVSAFYQEIFSNGVEKSIPTSSVFPEEALENCKTPFPGDECKVLKMSRVDVACGSGKNRRSTRLHNIKKKQKQHPECRGPFKHPDGEPSSTSKAMENHDSDTVVPDNVPASLPEISFDVEQDMSIKMSKVNDVHGSGRTRRSGRLHNAKDCQKKQPASGDPLKNPEKEQSSTLKVVEDHDLDTAVPDNVSTNLLEILSEAVEKSFPTSLVSPDKTTRDRNKALLEDERDMSIKTCRVNIVHGSSGNRRSARMHNVIPYQRKQPATGDPATHPDKDQISALAALELSDGGLESLSTPVQSKKGRVNTMSGQVTNKLSSRNSKTFSAKTEGCYKKKGRWLKQKRSHLREK*