Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0124480 | sp|Q9SY66|FRS11_ARATH | 40.336 | 238 | 116 | 7 | 45 | 262 | 21 | 252 | 1.35e-41 | 162 |
| Msa0124480 | sp|Q9SY66|FRS11_ARATH | 40.909 | 176 | 100 | 2 | 266 | 439 | 429 | 602 | 3.16e-36 | 146 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0124480 | A0A445GCJ8 | 64.833 | 600 | 138 | 12 | 9 | 545 | 12 | 601 | 0.0 | 735 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0124480 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0124480 | MtrunA17_Chr1g0198761 | 34.921 | 315 | 149 | 7 | 266 | 531 | 433 | 740 | 8.46e-50 | 183 |
| Msa0124480 | MtrunA17_Chr1g0198761 | 33.200 | 250 | 137 | 8 | 73 | 294 | 46 | 293 | 5.92e-28 | 118 |
| Msa0124480 | MtrunA17_Chr2g0276841 | 41.981 | 212 | 103 | 5 | 73 | 266 | 69 | 278 | 4.57e-42 | 160 |
| Msa0124480 | MtrunA17_Chr2g0276841 | 43.503 | 177 | 94 | 4 | 266 | 439 | 451 | 624 | 1.13e-38 | 150 |
| Msa0124480 | MtrunA17_Chr2g0294841 | 38.281 | 256 | 122 | 7 | 52 | 273 | 11 | 264 | 2.16e-37 | 147 |
| Msa0124480 | MtrunA17_Chr2g0294841 | 36.932 | 176 | 108 | 2 | 266 | 439 | 429 | 603 | 7.51e-31 | 127 |
| Msa0124480 | MtrunA17_Chr2g0290391 | 35.918 | 245 | 119 | 9 | 51 | 266 | 32 | 267 | 2.61e-30 | 125 |
| Msa0124480 | MtrunA17_Chr2g0290391 | 35.227 | 176 | 105 | 3 | 266 | 439 | 440 | 608 | 1.78e-28 | 120 |
| Msa0124480 | MtrunA17_Chr5g0431001 | 35.200 | 250 | 132 | 8 | 73 | 294 | 60 | 307 | 7.67e-30 | 122 |
| Msa0124480 | MtrunA17_Chr5g0431001 | 70.270 | 37 | 11 | 0 | 266 | 302 | 447 | 483 | 3.63e-11 | 65.5 |
| Msa0124480 | MtrunA17_Chr1g0202341 | 35.519 | 183 | 113 | 1 | 266 | 443 | 426 | 608 | 2.18e-25 | 110 |
| Msa0124480 | MtrunA17_Chr5g0448761 | 32.609 | 184 | 111 | 4 | 269 | 443 | 445 | 624 | 2.23e-22 | 101 |
| Msa0124480 | MtrunA17_Chr5g0448511 | 28.807 | 243 | 150 | 6 | 247 | 475 | 186 | 419 | 6.76e-22 | 99.4 |
| Msa0124480 | MtrunA17_Chr5g0440451 | 30.601 | 183 | 120 | 4 | 266 | 443 | 423 | 603 | 1.14e-20 | 96.3 |
| Msa0124480 | MtrunA17_Chr5g0441581 | 35.829 | 187 | 108 | 6 | 264 | 439 | 393 | 578 | 1.62e-19 | 92.4 |
| Msa0124480 | MtrunA17_Chr3g0080351 | 29.843 | 191 | 116 | 3 | 264 | 443 | 439 | 622 | 1.73e-19 | 92.4 |
| Msa0124480 | MtrunA17_Chr1g0207551 | 30.890 | 191 | 127 | 3 | 257 | 443 | 399 | 588 | 4.74e-19 | 91.3 |
| Msa0124480 | MtrunA17_Chr2g0296781 | 35.106 | 188 | 105 | 8 | 266 | 439 | 367 | 551 | 1.53e-18 | 89.4 |
| Msa0124480 | MtrunA17_Chr3g0118611 | 30.435 | 184 | 119 | 4 | 266 | 443 | 320 | 500 | 1.62e-18 | 89.4 |
| Msa0124480 | MtrunA17_Chr6g0477261 | 34.043 | 188 | 107 | 8 | 266 | 439 | 256 | 440 | 9.95e-18 | 86.7 |
| Msa0124480 | MtrunA17_Chr2g0329031 | 28.249 | 177 | 124 | 2 | 266 | 439 | 380 | 556 | 7.30e-16 | 80.9 |
| Msa0124480 | MtrunA17_Chr2g0291581 | 32.642 | 193 | 108 | 7 | 264 | 439 | 429 | 616 | 4.21e-15 | 78.6 |
| Msa0124480 | MtrunA17_Chr2g0324011 | 27.807 | 187 | 130 | 3 | 264 | 445 | 383 | 569 | 5.38e-14 | 74.7 |
| Msa0124480 | MtrunA17_Chr2g0308841 | 26.923 | 182 | 127 | 3 | 264 | 439 | 41 | 222 | 8.50e-14 | 71.6 |
| Msa0124480 | MtrunA17_Chr1g0196541 | 30.303 | 198 | 113 | 7 | 259 | 439 | 362 | 551 | 1.63e-13 | 73.6 |
| Msa0124480 | MtrunA17_Chr2g0287651 | 25.134 | 187 | 128 | 4 | 266 | 443 | 448 | 631 | 4.63e-12 | 68.9 |
| Msa0124480 | MtrunA17_Chr3g0082121 | 30.435 | 184 | 121 | 4 | 269 | 448 | 414 | 594 | 2.62e-11 | 66.2 |
| Msa0124480 | MtrunA17_Chr2g0287061 | 25.806 | 186 | 129 | 4 | 264 | 443 | 518 | 700 | 7.28e-11 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0124480 | AT1G10240.2 | 40.336 | 238 | 116 | 7 | 45 | 262 | 21 | 252 | 1.37e-42 | 162 |
| Msa0124480 | AT1G10240.2 | 40.909 | 176 | 100 | 2 | 266 | 439 | 429 | 602 | 3.21e-37 | 146 |
| Msa0124480 | AT1G10240.1 | 40.336 | 238 | 116 | 7 | 45 | 262 | 21 | 252 | 1.37e-42 | 162 |
| Msa0124480 | AT1G10240.1 | 40.909 | 176 | 100 | 2 | 266 | 439 | 429 | 602 | 3.21e-37 | 146 |
| Msa0124480 | AT5G28530.1 | 35.565 | 239 | 124 | 7 | 55 | 266 | 36 | 271 | 3.49e-30 | 125 |
| Msa0124480 | AT5G28530.1 | 29.444 | 180 | 114 | 4 | 266 | 439 | 444 | 616 | 4.11e-18 | 88.6 |
| Msa0124480 | AT5G28530.3 | 35.565 | 239 | 124 | 7 | 55 | 266 | 36 | 271 | 3.64e-30 | 125 |
| Msa0124480 | AT5G28530.3 | 29.545 | 176 | 115 | 3 | 266 | 439 | 444 | 612 | 1.99e-19 | 92.4 |
| Msa0124480 | AT5G28530.2 | 35.565 | 239 | 124 | 7 | 55 | 266 | 36 | 271 | 3.64e-30 | 125 |
| Msa0124480 | AT5G28530.2 | 29.545 | 176 | 115 | 3 | 266 | 439 | 444 | 612 | 1.99e-19 | 92.4 |
| Msa0124480 | AT5G28530.4 | 34.252 | 254 | 136 | 8 | 55 | 280 | 36 | 286 | 3.83e-30 | 125 |
| Msa0124480 | AT5G28530.4 | 29.444 | 180 | 114 | 4 | 266 | 439 | 381 | 553 | 2.20e-18 | 89.0 |
| Msa0124480 | AT5G28530.5 | 34.252 | 254 | 136 | 8 | 55 | 280 | 36 | 286 | 3.93e-30 | 125 |
| Msa0124480 | AT5G28530.5 | 29.545 | 176 | 115 | 3 | 266 | 439 | 381 | 549 | 9.20e-20 | 93.6 |
| Msa0124480 | AT4G38170.1 | 31.731 | 208 | 135 | 3 | 266 | 467 | 216 | 422 | 2.60e-25 | 110 |
| Msa0124480 | AT4G38180.1 | 32.843 | 204 | 122 | 5 | 266 | 457 | 451 | 651 | 4.07e-23 | 104 |
| Msa0124480 | AT3G22170.2 | 32.474 | 194 | 122 | 5 | 256 | 443 | 427 | 617 | 1.52e-19 | 93.2 |
| Msa0124480 | AT3G22170.1 | 32.474 | 194 | 122 | 5 | 256 | 443 | 427 | 617 | 1.52e-19 | 93.2 |
| Msa0124480 | AT3G06250.2 | 32.663 | 199 | 123 | 6 | 264 | 455 | 522 | 716 | 1.33e-17 | 87.0 |
| Msa0124480 | AT3G06250.1 | 32.663 | 199 | 123 | 6 | 264 | 455 | 522 | 716 | 1.33e-17 | 87.0 |
| Msa0124480 | AT3G06250.3 | 32.663 | 199 | 123 | 6 | 264 | 455 | 522 | 716 | 1.33e-17 | 87.0 |
| Msa0124480 | AT4G19990.3 | 30.319 | 188 | 121 | 3 | 264 | 443 | 373 | 558 | 8.06e-17 | 84.3 |
| Msa0124480 | AT4G19990.4 | 30.319 | 188 | 121 | 3 | 264 | 443 | 373 | 558 | 8.06e-17 | 84.3 |
| Msa0124480 | AT4G19990.7 | 30.319 | 188 | 121 | 3 | 264 | 443 | 387 | 572 | 9.15e-17 | 84.3 |
| Msa0124480 | AT4G19990.1 | 30.319 | 188 | 121 | 3 | 264 | 443 | 316 | 501 | 9.58e-17 | 84.0 |
| Msa0124480 | AT4G19990.5 | 30.319 | 188 | 121 | 3 | 264 | 443 | 373 | 558 | 1.07e-16 | 84.0 |
| Msa0124480 | AT4G19990.6 | 30.319 | 188 | 121 | 3 | 264 | 443 | 373 | 558 | 1.09e-16 | 84.0 |
| Msa0124480 | AT4G19990.2 | 30.319 | 188 | 121 | 3 | 264 | 443 | 373 | 558 | 1.09e-16 | 84.0 |
| Msa0124480 | AT1G76320.1 | 27.778 | 180 | 124 | 3 | 264 | 439 | 362 | 539 | 1.88e-16 | 83.2 |
| Msa0124480 | AT1G76320.4 | 27.778 | 180 | 124 | 3 | 264 | 439 | 362 | 539 | 1.88e-16 | 83.2 |
| Msa0124480 | AT1G76320.2 | 27.778 | 180 | 124 | 3 | 264 | 439 | 362 | 539 | 1.99e-16 | 83.2 |
| Msa0124480 | AT1G76320.3 | 27.778 | 180 | 124 | 3 | 264 | 439 | 362 | 539 | 2.12e-16 | 83.2 |
| Msa0124480 | AT1G52520.1 | 28.421 | 190 | 121 | 5 | 264 | 439 | 445 | 633 | 7.78e-16 | 81.3 |
| Msa0124480 | AT4G15090.1 | 29.282 | 181 | 122 | 4 | 264 | 439 | 425 | 604 | 8.79e-16 | 81.3 |
| Msa0124480 | AT4G15090.5 | 29.282 | 181 | 122 | 4 | 264 | 439 | 425 | 604 | 8.79e-16 | 81.3 |
| Msa0124480 | AT4G15090.3 | 29.282 | 181 | 122 | 4 | 264 | 439 | 425 | 604 | 8.79e-16 | 81.3 |
| Msa0124480 | AT4G15090.2 | 29.282 | 181 | 122 | 4 | 264 | 439 | 425 | 604 | 8.79e-16 | 81.3 |
| Msa0124480 | AT4G15090.4 | 29.282 | 181 | 122 | 4 | 264 | 439 | 425 | 604 | 8.79e-16 | 81.3 |
| Msa0124480 | AT5G18960.2 | 30.319 | 188 | 121 | 5 | 264 | 445 | 546 | 729 | 7.29e-15 | 78.2 |
| Msa0124480 | AT5G18960.1 | 30.319 | 188 | 121 | 5 | 264 | 445 | 546 | 729 | 7.29e-15 | 78.2 |
| Msa0124480 | AT2G27110.2 | 29.348 | 184 | 121 | 4 | 266 | 443 | 398 | 578 | 1.37e-14 | 77.4 |
| Msa0124480 | AT2G27110.1 | 29.348 | 184 | 121 | 4 | 266 | 443 | 398 | 578 | 1.37e-14 | 77.4 |
| Msa0124480 | AT2G27110.3 | 29.348 | 184 | 121 | 4 | 266 | 443 | 253 | 433 | 1.59e-14 | 77.0 |
| Msa0124480 | AT2G32250.6 | 23.166 | 259 | 172 | 7 | 197 | 439 | 301 | 548 | 3.86e-11 | 66.2 |
| Msa0124480 | AT2G32250.1 | 23.166 | 259 | 172 | 7 | 197 | 439 | 301 | 548 | 3.86e-11 | 66.2 |
| Msa0124480 | AT2G32250.3 | 23.166 | 259 | 172 | 7 | 197 | 439 | 301 | 548 | 3.86e-11 | 66.2 |
| Msa0124480 | AT2G32250.5 | 23.166 | 259 | 172 | 7 | 197 | 439 | 301 | 548 | 4.02e-11 | 66.2 |
| Msa0124480 | AT2G32250.2 | 23.166 | 259 | 172 | 7 | 197 | 439 | 301 | 548 | 4.20e-11 | 66.2 |
| Msa0124480 | AT2G32250.4 | 23.166 | 259 | 172 | 7 | 197 | 439 | 301 | 548 | 4.20e-11 | 66.2 |
| Msa0124480 | AT2G32250.8 | 23.166 | 259 | 172 | 7 | 197 | 439 | 260 | 507 | 4.75e-11 | 66.2 |
| Msa0124480 | AT2G32250.7 | 23.166 | 259 | 172 | 7 | 197 | 439 | 260 | 507 | 5.32e-11 | 65.9 |
Find 75 sgRNAs with CRISPR-Local
Find 143 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAATTGTGTTCCCTTTAT+TGG | 0.123228 | 1_3:+52266812 | Msa0124480:CDS |
| CGAATGTCCCTTTCAATAAA+TGG | 0.156974 | 1_3:-52267295 | None:intergenic |
| TATCTTCGTGATTATTTCTT+TGG | 0.163692 | 1_3:+52268103 | Msa0124480:CDS |
| CGACCTTTAGGTTTGGATTT+TGG | 0.167927 | 1_3:-52269007 | None:intergenic |
| GAAGATATTGCTTTAGAAAA+TGG | 0.185026 | 1_3:+52266644 | Msa0124480:CDS |
| CCTAAACGACGGCGACTTAA+AGG | 0.199216 | 1_3:+52269031 | Msa0124480:CDS |
| GTTGTAAGCTCTTTGAATTT+TGG | 0.244484 | 1_3:+52268783 | Msa0124480:CDS |
| CCTTTAAGTCGCCGTCGTTT+AGG | 0.263372 | 1_3:-52269031 | None:intergenic |
| GCAGAAAACATGCAGTGTTT+TGG | 0.283471 | 1_3:+52268738 | Msa0124480:CDS |
| TATTCAATTGATCATGAAAA+TGG | 0.285695 | 1_3:+52268677 | Msa0124480:CDS |
| TAAATGTGTACCTAAACTTT+TGG | 0.293742 | 1_3:-52269199 | None:intergenic |
| CGTCGTTTAGGACGACCTTT+AGG | 0.309582 | 1_3:-52269019 | None:intergenic |
| AATTCTTCTTCCAAAAGTTT+AGG | 0.313803 | 1_3:+52269189 | Msa0124480:CDS |
| AAATGGTTGTTCAGAAATAA+TGG | 0.340072 | 1_3:+52266661 | Msa0124480:CDS |
| TTTAACCGACTTTGTAAAAC+AGG | 0.371626 | 1_3:+52268198 | Msa0124480:CDS |
| CGTGATGGCAAGTAGTTAGA+AGG | 0.372743 | 1_3:-52268863 | None:intergenic |
| ATTTGAGAGAAGTGATGTTA+TGG | 0.378695 | 1_3:+52267345 | Msa0124480:CDS |
| TAGAGCCTCCTTGAAATTCA+TGG | 0.405110 | 1_3:+52266976 | Msa0124480:CDS |
| GTATGAAATCAGAAACTATT+GGG | 0.418531 | 1_3:+52268072 | Msa0124480:intron |
| GTAGATTCACAAACAAGAAT+GGG | 0.422387 | 1_3:+52266915 | Msa0124480:CDS |
| TGTATGAAATCAGAAACTAT+TGG | 0.424688 | 1_3:+52268071 | Msa0124480:intron |
| TTTAGGACGACCTTTAGGTT+TGG | 0.425449 | 1_3:-52269014 | None:intergenic |
| CTTCGTGATTATTTCTTTGG+TGG | 0.427885 | 1_3:+52268106 | Msa0124480:CDS |
| TTTGCCTTCTTTCCCTTTAG+CGG | 0.431590 | 1_3:-52269127 | None:intergenic |
| TGATGGTTCCATGAATTTCA+AGG | 0.441442 | 1_3:-52266984 | None:intergenic |
| CATTGTCACATGTCCGCTAA+AGG | 0.446736 | 1_3:+52269114 | Msa0124480:CDS |
| TTCGTGATTATTTCTTTGGT+GGG | 0.450960 | 1_3:+52268107 | Msa0124480:CDS |
| CGGTATTATAAAGAGGTTAA+CGG | 0.457669 | 1_3:+52268716 | Msa0124480:CDS |
| ATTAATACTCTCCGATCTTC+CGG | 0.458636 | 1_3:-52268140 | None:intergenic |
| GGTAGATTCACAAACAAGAA+TGG | 0.461401 | 1_3:+52266914 | Msa0124480:CDS |
| ATTGTCACATGTCCGCTAAA+GGG | 0.468125 | 1_3:+52269115 | Msa0124480:CDS |
| AATGAAGAAGGTGATAAAGA+TGG | 0.470337 | 1_3:+52266776 | Msa0124480:intron |
| TAACCTTCCATTTATTGAAA+GGG | 0.475583 | 1_3:+52267288 | Msa0124480:CDS |
| ATGGTAATGTGTTTGTGCTT+CGG | 0.482748 | 1_3:+52268696 | Msa0124480:CDS |
| GTAACCTTCCATTTATTGAA+AGG | 0.500947 | 1_3:+52267287 | Msa0124480:CDS |
| TTTAGTGAAGGACTTGTTAG+TGG | 0.507871 | 1_3:+52266694 | Msa0124480:CDS |
| GAAGGTGATAAAGATGGGTC+TGG | 0.517282 | 1_3:+52266782 | Msa0124480:intron |
| GTTGTAACTCGCCACTCAGT+TGG | 0.517634 | 1_3:-52267091 | None:intergenic |
| ATGTCCGCTAAAGGGAAAGA+AGG | 0.522488 | 1_3:+52269123 | Msa0124480:CDS |
| TGTGCTTCGGTATTATAAAG+AGG | 0.524446 | 1_3:+52268709 | Msa0124480:CDS |
| AGAGCATAGAAATAGAGAAT+CGG | 0.534571 | 1_3:+52267009 | Msa0124480:CDS |
| TTGTTAGTGGTTGATAACAT+AGG | 0.538715 | 1_3:+52266707 | Msa0124480:CDS |
| CCTCCAAAATCCAAACCTAA+AGG | 0.542127 | 1_3:+52269004 | Msa0124480:CDS |
| ACAAGAATGGGGTTATGAGC+AGG | 0.542872 | 1_3:+52266927 | Msa0124480:CDS |
| ATGAAGAAGGTGATAAAGAT+GGG | 0.545139 | 1_3:+52266777 | Msa0124480:intron |
| GATGACAACCACCGGAAGAT+CGG | 0.546284 | 1_3:+52268129 | Msa0124480:CDS |
| AGTTTCAAGAGGAGTTTGAA+AGG | 0.562176 | 1_3:+52268645 | Msa0124480:CDS |
| TCAGAGATAGTTCGATAAGC+CGG | 0.569841 | 1_3:-52267169 | None:intergenic |
| TCACATAACATGAACACTTG+TGG | 0.569949 | 1_3:+52269067 | Msa0124480:CDS |
| CTGATTGGTTTAGCAAACCA+TGG | 0.574779 | 1_3:-52267132 | None:intergenic |
| CTTCTAACTACTTGCCATCA+CGG | 0.575563 | 1_3:+52268864 | Msa0124480:CDS |
| TGTCCCTTTCAATAAATGGA+AGG | 0.578784 | 1_3:-52267291 | None:intergenic |
| ATACACTTGATGAGGAAAGA+AGG | 0.579288 | 1_3:+52267425 | Msa0124480:CDS |
| TTTGGTGGGATGACAACCAC+CGG | 0.581206 | 1_3:+52268121 | Msa0124480:CDS |
| AAGAGATATGCTTATCAACA+AGG | 0.596535 | 1_3:+52266875 | Msa0124480:CDS |
| AAACGACGGCGACTTAAAGG+TGG | 0.596721 | 1_3:+52269034 | Msa0124480:CDS |
| TTTGTTGTTGAACATAACCA+TGG | 0.597979 | 1_3:+52267115 | Msa0124480:CDS |
| ATGATCAATTGAATATTGAG+CGG | 0.600490 | 1_3:-52268669 | None:intergenic |
| AATACTCTCCGATCTTCCGG+TGG | 0.600856 | 1_3:-52268137 | None:intergenic |
| GGTTGAGCATGATTTAGTGA+AGG | 0.607141 | 1_3:+52266682 | Msa0124480:CDS |
| CTATGAGCTTGCTCTTGTAG+AGG | 0.612240 | 1_3:-52268596 | None:intergenic |
| ATATGCATATACACTTGATG+AGG | 0.621673 | 1_3:+52267417 | Msa0124480:CDS |
| GTGATGGCAAGTAGTTAGAA+GGG | 0.633198 | 1_3:-52268862 | None:intergenic |
| TGTGGATTATGCAAAGGTGA+AGG | 0.636366 | 1_3:+52269085 | Msa0124480:CDS |
| GGATCTTCTGAAGTATTGCG+AGG | 0.640419 | 1_3:+52267366 | Msa0124480:CDS |
| AGAGATATGCTTATCAACAA+GGG | 0.641950 | 1_3:+52266876 | Msa0124480:CDS |
| ATGATATATTTCCAACTGAG+TGG | 0.648662 | 1_3:+52267080 | Msa0124480:CDS |
| GCTTGAAGCAACCACCGTGA+TGG | 0.657308 | 1_3:-52268878 | None:intergenic |
| ATAATGTTAGAGAAGTGCAA+AGG | 0.660770 | 1_3:+52268551 | Msa0124480:CDS |
| TGGTTTGCTAAACCAATCAG+AGG | 0.671864 | 1_3:+52267135 | Msa0124480:CDS |
| TAGATTCACAAACAAGAATG+GGG | 0.673558 | 1_3:+52266916 | Msa0124480:CDS |
| AACACTTGTGGATTATGCAA+AGG | 0.684041 | 1_3:+52269079 | Msa0124480:CDS |
| CTAAAGGTCGTCCTAAACGA+CGG | 0.691619 | 1_3:+52269020 | Msa0124480:CDS |
| TGGTTCCATGAATTTCAAGG+AGG | 0.710462 | 1_3:-52266981 | None:intergenic |
| CTAACTACTTGCCATCACGG+TGG | 0.742949 | 1_3:+52268867 | Msa0124480:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CGTATTTTTTTATTAAAAAA+AGG | + | chr1_3:52267205-52267224 | Msa0124480:intron | 10.0% |
| !!! | ATTTTTTTATTAAAAAAAGG+TGG | + | chr1_3:52267208-52267227 | Msa0124480:intron | 10.0% |
| !!! | TTTAATTTTAAAAGTTAATC+CGG | + | chr1_3:52268421-52268440 | Msa0124480:intron | 10.0% |
| !!! | ATCAAAACTTATTTTATTGT+TGG | - | chr1_3:52266606-52266625 | None:intergenic | 15.0% |
| !! | TTAAAATAATTCTGTTTCAT+GGG | - | chr1_3:52268400-52268419 | None:intergenic | 15.0% |
| !! | ATTAAAATAATTCTGTTTCA+TGG | - | chr1_3:52268401-52268420 | None:intergenic | 15.0% |
| !! | AATATTGTAACAAATGAAGA+AGG | + | chr1_3:52266764-52266783 | Msa0124480:intron | 20.0% |
| !! | AGAAAAATTTGACCAATAAA+GGG | - | chr1_3:52266827-52266846 | None:intergenic | 20.0% |
| !! | AAGAAAAATTTGACCAATAA+AGG | - | chr1_3:52266828-52266847 | None:intergenic | 20.0% |
| !! | TTAATAAAAAAATACGCTCA+CGG | - | chr1_3:52267201-52267220 | None:intergenic | 20.0% |
| !! | ACGCATATGTATATAAAAAA+AGG | - | chr1_3:52267714-52267733 | None:intergenic | 20.0% |
| !!! | TTTTTATGAGTTGTATAAGT+TGG | + | chr1_3:52267967-52267986 | Msa0124480:intron | 20.0% |
| !!! | ATTTCTGTAGACAATTTTTT+CGG | + | chr1_3:52268364-52268383 | Msa0124480:intron | 20.0% |
| !! | TATTCAATTGATCATGAAAA+TGG | + | chr1_3:52268677-52268696 | Msa0124480:CDS | 20.0% |
| !!! | GAAGATATTGCTTTAGAAAA+TGG | + | chr1_3:52266644-52266663 | Msa0124480:CDS | 25.0% |
| ! | AAATGGTTGTTCAGAAATAA+TGG | + | chr1_3:52266661-52266680 | Msa0124480:CDS | 25.0% |
| ! | ATTAAAAAAAGGTGGTCTTT+CGG | + | chr1_3:52267216-52267235 | Msa0124480:intron | 25.0% |
| ! | TAACCTTCCATTTATTGAAA+GGG | + | chr1_3:52267288-52267307 | Msa0124480:CDS | 25.0% |
| ! | TTTGATTGGTACCAAAAATA+TGG | + | chr1_3:52267481-52267500 | Msa0124480:intron | 25.0% |
| ! | TGTATTTGATACTACATACA+AGG | + | chr1_3:52267513-52267532 | Msa0124480:intron | 25.0% |
| ! | AATTCTTATGAAATGCCATT+TGG | + | chr1_3:52267538-52267557 | Msa0124480:intron | 25.0% |
| !!! | TAACTTTCAAGTTTAGTTCT+TGG | + | chr1_3:52267900-52267919 | Msa0124480:intron | 25.0% |
| ! | TTGTATTTAGCAACAACATT+TGG | - | chr1_3:52268022-52268041 | None:intergenic | 25.0% |
| ! | TGTATGAAATCAGAAACTAT+TGG | + | chr1_3:52268071-52268090 | Msa0124480:intron | 25.0% |
| ! | GTATGAAATCAGAAACTATT+GGG | + | chr1_3:52268072-52268091 | Msa0124480:intron | 25.0% |
| !! | TATCTTCGTGATTATTTCTT+TGG | + | chr1_3:52268103-52268122 | Msa0124480:CDS | 25.0% |
| !! | TTCTTTTCAAAAGTTTCAAG+AGG | + | chr1_3:52268634-52268653 | Msa0124480:CDS | 25.0% |
| ! | ATGATCAATTGAATATTGAG+CGG | - | chr1_3:52268672-52268691 | None:intergenic | 25.0% |
| !! | TTGTAAGCTCTTTGAATTTT+GGG | + | chr1_3:52268784-52268803 | Msa0124480:CDS | 25.0% |
| !!! | TAAACTTTTGGAAGAAGAAT+TGG | - | chr1_3:52269190-52269209 | None:intergenic | 25.0% |
| !! | TTGTTAGTGGTTGATAACAT+AGG | + | chr1_3:52266707-52266726 | Msa0124480:CDS | 30.0% |
| AATGAAGAAGGTGATAAAGA+TGG | + | chr1_3:52266776-52266795 | Msa0124480:intron | 30.0% | |
| ATGAAGAAGGTGATAAAGAT+GGG | + | chr1_3:52266777-52266796 | Msa0124480:intron | 30.0% | |
| ACAAATTGTGTTCCCTTTAT+TGG | + | chr1_3:52266812-52266831 | Msa0124480:CDS | 30.0% | |
| AAGAGATATGCTTATCAACA+AGG | + | chr1_3:52266875-52266894 | Msa0124480:CDS | 30.0% | |
| AGAGATATGCTTATCAACAA+GGG | + | chr1_3:52266876-52266895 | Msa0124480:CDS | 30.0% | |
| CAAGGGTTCTCAATAAAAAA+AGG | + | chr1_3:52266893-52266912 | Msa0124480:CDS | 30.0% | |
| GTAGATTCACAAACAAGAAT+GGG | + | chr1_3:52266915-52266934 | Msa0124480:CDS | 30.0% | |
| TAGATTCACAAACAAGAATG+GGG | + | chr1_3:52266916-52266935 | Msa0124480:CDS | 30.0% | |
| !!! | CTATTTCTATGCTCTTTTGA+TGG | - | chr1_3:52267004-52267023 | None:intergenic | 30.0% |
| AGAGCATAGAAATAGAGAAT+CGG | + | chr1_3:52267009-52267028 | Msa0124480:CDS | 30.0% | |
| ATGATATATTTCCAACTGAG+TGG | + | chr1_3:52267080-52267099 | Msa0124480:CDS | 30.0% | |
| !! | TTTGTTGTTGAACATAACCA+TGG | + | chr1_3:52267115-52267134 | Msa0124480:CDS | 30.0% |
| GTAACCTTCCATTTATTGAA+AGG | + | chr1_3:52267287-52267306 | Msa0124480:CDS | 30.0% | |
| ATTTGAGAGAAGTGATGTTA+TGG | + | chr1_3:52267345-52267364 | Msa0124480:CDS | 30.0% | |
| ATATGCATATACACTTGATG+AGG | + | chr1_3:52267417-52267436 | Msa0124480:CDS | 30.0% | |
| !! | AATGCCATTTGGAATTTTTG+TGG | + | chr1_3:52267549-52267568 | Msa0124480:intron | 30.0% |
| !! | ATGCCATTTGGAATTTTTGT+GGG | + | chr1_3:52267550-52267569 | Msa0124480:intron | 30.0% |
| ATACCCACAAAAATTCCAAA+TGG | - | chr1_3:52267556-52267575 | None:intergenic | 30.0% | |
| TTTGTGGGTATGAATAATCA+TGG | + | chr1_3:52267565-52267584 | Msa0124480:intron | 30.0% | |
| TGATCGGTTAATATAGTCTT+TGG | - | chr1_3:52267809-52267828 | None:intergenic | 30.0% | |
| TATTAACCGATCAAGATCTA+TGG | + | chr1_3:52267816-52267835 | Msa0124480:intron | 30.0% | |
| TTTCATCCATAGATCTTGAT+CGG | - | chr1_3:52267825-52267844 | None:intergenic | 30.0% | |
| TTCGAGACAAATATGCAAAA+TGG | + | chr1_3:52267936-52267955 | Msa0124480:intron | 30.0% | |
| CAGTCAAATAAACATGTGAA+AGG | + | chr1_3:52268046-52268065 | Msa0124480:intron | 30.0% | |
| ! | TTCGTGATTATTTCTTTGGT+GGG | + | chr1_3:52268107-52268126 | Msa0124480:CDS | 30.0% |
| ! | TTTAACCGACTTTGTAAAAC+AGG | + | chr1_3:52268198-52268217 | Msa0124480:CDS | 30.0% |
| ! | TCATACCTGTTTTACAAAGT+CGG | - | chr1_3:52268206-52268225 | None:intergenic | 30.0% |
| TAGTGTATTCTTGAAAATGC+TGG | - | chr1_3:52268337-52268356 | None:intergenic | 30.0% | |
| ! | ATAATGTTAGAGAAGTGCAA+AGG | + | chr1_3:52268551-52268570 | Msa0124480:CDS | 30.0% |
| CGGTATTATAAAGAGGTTAA+CGG | + | chr1_3:52268716-52268735 | Msa0124480:CDS | 30.0% | |
| ! | GTTGTAAGCTCTTTGAATTT+TGG | + | chr1_3:52268783-52268802 | Msa0124480:CDS | 30.0% |
| ! | TGTAAGCTCTTTGAATTTTG+GGG | + | chr1_3:52268785-52268804 | Msa0124480:CDS | 30.0% |
| !! | TTTAGTGAAGGACTTGTTAG+TGG | + | chr1_3:52266694-52266713 | Msa0124480:CDS | 35.0% |
| GGTAGATTCACAAACAAGAA+TGG | + | chr1_3:52266914-52266933 | Msa0124480:CDS | 35.0% | |
| !!! | GATTTTTTTTGCCATCGTGA+AGG | + | chr1_3:52266953-52266972 | Msa0124480:intron | 35.0% |
| TGATGGTTCCATGAATTTCA+AGG | - | chr1_3:52266987-52267006 | None:intergenic | 35.0% | |
| CTAGAGAAAAATGTGAAGCA+TGG | + | chr1_3:52267265-52267284 | Msa0124480:CDS | 35.0% | |
| TGTCCCTTTCAATAAATGGA+AGG | - | chr1_3:52267294-52267313 | None:intergenic | 35.0% | |
| CGAATGTCCCTTTCAATAAA+TGG | - | chr1_3:52267298-52267317 | None:intergenic | 35.0% | |
| ATACACTTGATGAGGAAAGA+AGG | + | chr1_3:52267425-52267444 | Msa0124480:CDS | 35.0% | |
| !!! | GAAGGTTGGAGCATATTTTT+TGG | + | chr1_3:52267443-52267462 | Msa0124480:intron | 35.0% |
| !!! | GGAGCATATTTTTTGGTCAT+CGG | + | chr1_3:52267450-52267469 | Msa0124480:intron | 35.0% |
| ! | CAACAACATCGCCATATTTT+TGG | - | chr1_3:52267495-52267514 | None:intergenic | 35.0% |
| ! | TTTTTGGATGTGCACTCTTA+CGG | + | chr1_3:52267599-52267618 | Msa0124480:intron | 35.0% |
| TCGGTTAATATAGTCTTTGG+TGG | - | chr1_3:52267806-52267825 | None:intergenic | 35.0% | |
| CAAAACACAGTTTCTGCATA+TGG | + | chr1_3:52267873-52267892 | Msa0124480:intron | 35.0% | |
| TGCATATTTGTCTCGAAGTA+TGG | - | chr1_3:52267933-52267952 | None:intergenic | 35.0% | |
| GTGAAGAATTTGAGCATCAA+TGG | + | chr1_3:52267996-52268015 | Msa0124480:intron | 35.0% | |
| ! | CTTCGTGATTATTTCTTTGG+TGG | + | chr1_3:52268106-52268125 | Msa0124480:CDS | 35.0% |
| ATTAATACTCTCCGATCTTC+CGG | - | chr1_3:52268143-52268162 | None:intergenic | 35.0% | |
| GACATGTACAAAGTTTCATG+GGG | - | chr1_3:52268294-52268313 | None:intergenic | 35.0% | |
| GGACATGTACAAAGTTTCAT+GGG | - | chr1_3:52268295-52268314 | None:intergenic | 35.0% | |
| GGAGGAAGAAAATGAGAAAT+GGG | - | chr1_3:52268316-52268335 | None:intergenic | 35.0% | |
| TGGAGGAAGAAAATGAGAAA+TGG | - | chr1_3:52268317-52268336 | None:intergenic | 35.0% | |
| TGTATTCTTGAAAATGCTGG+AGG | - | chr1_3:52268334-52268353 | None:intergenic | 35.0% | |
| GTCTACAGAAATAGTAACAC+CGG | - | chr1_3:52268443-52268462 | None:intergenic | 35.0% | |
| AGTTTCAAGAGGAGTTTGAA+AGG | + | chr1_3:52268645-52268664 | Msa0124480:CDS | 35.0% | |
| ! | ATGGTAATGTGTTTGTGCTT+CGG | + | chr1_3:52268696-52268715 | Msa0124480:CDS | 35.0% |
| TGTGCTTCGGTATTATAAAG+AGG | + | chr1_3:52268709-52268728 | Msa0124480:CDS | 35.0% | |
| ! | CAGAAAACATGCAGTGTTTT+GGG | + | chr1_3:52268739-52268758 | Msa0124480:CDS | 35.0% |
| ! | GTAAGCTCTTTGAATTTTGG+GGG | + | chr1_3:52268786-52268805 | Msa0124480:CDS | 35.0% |
| TCACATAACATGAACACTTG+TGG | + | chr1_3:52269067-52269086 | Msa0124480:CDS | 35.0% | |
| ! | AACACTTGTGGATTATGCAA+AGG | + | chr1_3:52269079-52269098 | Msa0124480:CDS | 35.0% |
| GGTTGAGCATGATTTAGTGA+AGG | + | chr1_3:52266682-52266701 | Msa0124480:CDS | 40.0% | |
| TAGAGCCTCCTTGAAATTCA+TGG | + | chr1_3:52266976-52266995 | Msa0124480:CDS | 40.0% | |
| TGGTTCCATGAATTTCAAGG+AGG | - | chr1_3:52266984-52267003 | None:intergenic | 40.0% | |
| !! | CTGATTGGTTTAGCAAACCA+TGG | - | chr1_3:52267135-52267154 | None:intergenic | 40.0% |
| !! | TGGTTTGCTAAACCAATCAG+AGG | + | chr1_3:52267135-52267154 | Msa0124480:CDS | 40.0% |
| GTAAAAAACGCACCTCTGAT+TGG | - | chr1_3:52267150-52267169 | None:intergenic | 40.0% | |
| !!! | ATCAGAGGTGCGTTTTTTAC+CGG | + | chr1_3:52267150-52267169 | Msa0124480:CDS | 40.0% |
| TCAGAGATAGTTCGATAAGC+CGG | - | chr1_3:52267172-52267191 | None:intergenic | 40.0% | |
| ACTTGATGAGGAAAGAAGGT+TGG | + | chr1_3:52267429-52267448 | Msa0124480:intron | 40.0% | |
| !!! | CATCGGCTTCTTGTTTTGAT+TGG | + | chr1_3:52267467-52267486 | Msa0124480:intron | 40.0% |
| ! | CATGGAAAGACCGTACTTTT+TGG | + | chr1_3:52267583-52267602 | Msa0124480:intron | 40.0% |
| GAGTGCACATCCAAAAAGTA+CGG | - | chr1_3:52267596-52267615 | None:intergenic | 40.0% | |
| AAACTGTGTCTGCATTTCGT+TGG | + | chr1_3:52267626-52267645 | Msa0124480:intron | 40.0% | |
| ! | TGCATTTCGTTGGCTAATGA+AGG | + | chr1_3:52267636-52267655 | Msa0124480:intron | 40.0% |
| GGAGAGTTTCTTAAGGATGA+TGG | - | chr1_3:52268273-52268292 | None:intergenic | 40.0% | |
| TTCATGGGGAGAGTTTCTTA+AGG | - | chr1_3:52268280-52268299 | None:intergenic | 40.0% | |
| GGGACATGTACAAAGTTTCA+TGG | - | chr1_3:52268296-52268315 | None:intergenic | 40.0% | |
| ! | GCAGAAAACATGCAGTGTTT+TGG | + | chr1_3:52268738-52268757 | Msa0124480:CDS | 40.0% |
| !!! | AAACATGCAGTGTTTTGGGA+TGG | + | chr1_3:52268743-52268762 | Msa0124480:CDS | 40.0% |
| GTGATGGCAAGTAGTTAGAA+GGG | - | chr1_3:52268865-52268884 | None:intergenic | 40.0% | |
| CTTCTAACTACTTGCCATCA+CGG | + | chr1_3:52268864-52268883 | Msa0124480:CDS | 40.0% | |
| !! | CCTTTAGGTTTGGATTTTGG+AGG | - | chr1_3:52269007-52269026 | None:intergenic | 40.0% |
| CCTCCAAAATCCAAACCTAA+AGG | + | chr1_3:52269004-52269023 | Msa0124480:CDS | 40.0% | |
| !! | CGACCTTTAGGTTTGGATTT+TGG | - | chr1_3:52269010-52269029 | None:intergenic | 40.0% |
| ! | TTTAGGACGACCTTTAGGTT+TGG | - | chr1_3:52269017-52269036 | None:intergenic | 40.0% |
| ! | TGTGGATTATGCAAAGGTGA+AGG | + | chr1_3:52269085-52269104 | Msa0124480:CDS | 40.0% |
| ATTGTCACATGTCCGCTAAA+GGG | + | chr1_3:52269115-52269134 | Msa0124480:CDS | 40.0% | |
| TTTGCCTTCTTTCCCTTTAG+CGG | - | chr1_3:52269130-52269149 | None:intergenic | 40.0% | |
| GAAGGTGATAAAGATGGGTC+TGG | + | chr1_3:52266782-52266801 | Msa0124480:intron | 45.0% | |
| ACAAGAATGGGGTTATGAGC+AGG | + | chr1_3:52266927-52266946 | Msa0124480:CDS | 45.0% | |
| !! | GGATCTTCTGAAGTATTGCG+AGG | + | chr1_3:52267366-52267385 | Msa0124480:CDS | 45.0% |
| ! | CTATGAGCTTGCTCTTGTAG+AGG | - | chr1_3:52268599-52268618 | None:intergenic | 45.0% |
| !!! | TGCAGTGTTTTGGGATGGTA+AGG | + | chr1_3:52268748-52268767 | Msa0124480:CDS | 45.0% |
| CGTGATGGCAAGTAGTTAGA+AGG | - | chr1_3:52268866-52268885 | None:intergenic | 45.0% | |
| CTAAAGGTCGTCCTAAACGA+CGG | + | chr1_3:52269020-52269039 | Msa0124480:CDS | 45.0% | |
| CATTGTCACATGTCCGCTAA+AGG | + | chr1_3:52269114-52269133 | Msa0124480:CDS | 45.0% | |
| ATGTCCGCTAAAGGGAAAGA+AGG | + | chr1_3:52269123-52269142 | Msa0124480:CDS | 45.0% | |
| !!! | AATTATTTTTCATTAATTTT+AGG | + | chr1_3:52268484-52268503 | Msa0124480:intron | 5.0% |
| GTTGTAACTCGCCACTCAGT+TGG | - | chr1_3:52267094-52267113 | None:intergenic | 50.0% | |
| TTTGGTGGGATGACAACCAC+CGG | + | chr1_3:52268121-52268140 | Msa0124480:CDS | 50.0% | |
| GATGACAACCACCGGAAGAT+CGG | + | chr1_3:52268129-52268148 | Msa0124480:CDS | 50.0% | |
| AATACTCTCCGATCTTCCGG+TGG | - | chr1_3:52268140-52268159 | None:intergenic | 50.0% | |
| CTAACTACTTGCCATCACGG+TGG | + | chr1_3:52268867-52268886 | Msa0124480:CDS | 50.0% | |
| ! | CGTCGTTTAGGACGACCTTT+AGG | - | chr1_3:52269022-52269041 | None:intergenic | 50.0% |
| CCTTTAAGTCGCCGTCGTTT+AGG | - | chr1_3:52269034-52269053 | None:intergenic | 50.0% | |
| CCTAAACGACGGCGACTTAA+AGG | + | chr1_3:52269031-52269050 | Msa0124480:CDS | 50.0% | |
| AAACGACGGCGACTTAAAGG+TGG | + | chr1_3:52269034-52269053 | Msa0124480:CDS | 50.0% | |
| AGGAGGCTCTACCTTCACGA+TGG | - | chr1_3:52266967-52266986 | None:intergenic | 55.0% | |
| GCTTGAAGCAACCACCGTGA+TGG | - | chr1_3:52268881-52268900 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_3 | gene | 52266578 | 52269210 | 52266578 | ID=Msa0124480;Name=Msa0124480 |
| chr1_3 | mRNA | 52266578 | 52269210 | 52266578 | ID=Msa0124480-mRNA-1;Parent=Msa0124480;Name=Msa0124480-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0|0|1|0.2|0.16|6|0|545 |
| chr1_3 | exon | 52266578 | 52266758 | 52266578 | ID=Msa0124480-mRNA-1:exon:15994;Parent=Msa0124480-mRNA-1 |
| chr1_3 | exon | 52266786 | 52266950 | 52266786 | ID=Msa0124480-mRNA-1:exon:15995;Parent=Msa0124480-mRNA-1 |
| chr1_3 | exon | 52266975 | 52267205 | 52266975 | ID=Msa0124480-mRNA-1:exon:15996;Parent=Msa0124480-mRNA-1 |
| chr1_3 | exon | 52267227 | 52267446 | 52267227 | ID=Msa0124480-mRNA-1:exon:15997;Parent=Msa0124480-mRNA-1 |
| chr1_3 | exon | 52268084 | 52268219 | 52268084 | ID=Msa0124480-mRNA-1:exon:15998;Parent=Msa0124480-mRNA-1 |
| chr1_3 | exon | 52268506 | 52269210 | 52268506 | ID=Msa0124480-mRNA-1:exon:15999;Parent=Msa0124480-mRNA-1 |
| chr1_3 | CDS | 52266578 | 52266758 | 52266578 | ID=Msa0124480-mRNA-1:cds;Parent=Msa0124480-mRNA-1 |
| chr1_3 | CDS | 52266786 | 52266950 | 52266786 | ID=Msa0124480-mRNA-1:cds;Parent=Msa0124480-mRNA-1 |
| chr1_3 | CDS | 52266975 | 52267205 | 52266975 | ID=Msa0124480-mRNA-1:cds;Parent=Msa0124480-mRNA-1 |
| chr1_3 | CDS | 52267227 | 52267446 | 52267227 | ID=Msa0124480-mRNA-1:cds;Parent=Msa0124480-mRNA-1 |
| chr1_3 | CDS | 52268084 | 52268219 | 52268084 | ID=Msa0124480-mRNA-1:cds;Parent=Msa0124480-mRNA-1 |
| chr1_3 | CDS | 52268506 | 52269210 | 52268506 | ID=Msa0124480-mRNA-1:cds;Parent=Msa0124480-mRNA-1 |
| Gene Sequence |
| Protein sequence |
