AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0125190


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0125190 MtrunA17_Chr5g0399781 52.830 53 24 1 4 56 13 64 1.51e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 21 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCTTGTGGTTTACATTTCA+AGG 0.339580 1_3:+53563085 Msa0125190:CDS
ATTGGTGTCGGCGTCTCTAT+TGG 0.349108 1_3:-53564072 None:intergenic
ATTGGTGTCGTCATCTCTAT+TGG 0.371711 1_3:-53564054 None:intergenic
CCACAAGCGTTACACAATAT+TGG 0.380139 1_3:-53563071 None:intergenic
TTTCAACAGAAACTCAAGTT+TGG 0.389578 1_3:+53563027 Msa0125190:CDS
AATCTTTCAAACCAAACTTC+AGG 0.411413 1_3:+53563200 Msa0125190:CDS
CCAAGCAATGAGATAGGTCT+TGG 0.425187 1_3:+53564201 Msa0125190:CDS
CACAATATTGGCGTCTCGTA+TGG 0.440494 1_3:-53563059 None:intergenic
TCAAACAACAAAATATCGTC+AGG 0.502407 1_3:-53564159 None:intergenic
TCTTGGATGCATTCGTCTAA+AGG 0.502885 1_3:+53564218 Msa0125190:CDS
TTTATACCAAGCAATGAGAT+AGG 0.511374 1_3:+53564195 Msa0125190:CDS
CCAATATTGTGTAACGCTTG+TGG 0.532300 1_3:+53563071 Msa0125190:CDS
GAAACTCAAGTTTGGCGCAA+AGG 0.544030 1_3:+53563035 Msa0125190:CDS
CCAAGACCTATCTCATTGCT+TGG 0.545705 1_3:-53564201 None:intergenic
GGTTTACATTTCAAGGCGAA+AGG 0.567422 1_3:+53563092 Msa0125190:CDS
AATAGAGACGCCGACACCAA+TGG 0.575440 1_3:+53564074 Msa0125190:CDS
TCTTCGGGAGATAGTTAGCA+AGG 0.579240 1_3:-53563118 None:intergenic
GGAAACTCATCCATTGGTGT+CGG 0.599667 1_3:-53564084 None:intergenic
AGCCCTGCAAAATCAGTCAT+CGG 0.602139 1_3:+53563142 Msa0125190:CDS
GATACTTCTACAGATCAAGA+TGG 0.615323 1_3:+53564246 Msa0125190:CDS
CTTCGGGAGATAGTTAGCAA+GGG 0.642252 1_3:-53563117 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAGATGATATAAATTGAA+GGG - chr1_3:53563301-53563320 None:intergenic 15.0%
!! AAATAGATGATATAAATTGA+AGG - chr1_3:53563302-53563321 None:intergenic 15.0%
!!! ACATTGTAAGAATATTTTTA+AGG + chr1_3:53563487-53563506 Msa0125190:intron 15.0%
!!! CATTGTAAGAATATTTTTAA+GGG + chr1_3:53563488-53563507 Msa0125190:intron 15.0%
!!! ATTGTAAGAATATTTTTAAG+GGG + chr1_3:53563489-53563508 Msa0125190:intron 15.0%
!! TTAAAATATGAAACTTCATT+TGG - chr1_3:53563676-53563695 None:intergenic 15.0%
!! AATAGATGATATAAATTGAA+GGG - chr1_3:53563301-53563320 None:intergenic 15.0%
!! AAATAGATGATATAAATTGA+AGG - chr1_3:53563302-53563321 None:intergenic 15.0%
!!! ACATTGTAAGAATATTTTTA+AGG + chr1_3:53563487-53563506 Msa0125190:intron 15.0%
!!! CATTGTAAGAATATTTTTAA+GGG + chr1_3:53563488-53563507 Msa0125190:intron 15.0%
!!! ATTGTAAGAATATTTTTAAG+GGG + chr1_3:53563489-53563508 Msa0125190:intron 15.0%
!! TTAAAATATGAAACTTCATT+TGG - chr1_3:53563676-53563695 None:intergenic 15.0%
!!! AATTTTTATTACCTGAAGTT+TGG - chr1_3:53563214-53563233 None:intergenic 20.0%
!!! AATTTTTATTACCTGAAGTT+TGG - chr1_3:53563214-53563233 None:intergenic 20.0%
!! TTTACATTTGGTGAAATATG+GGG - chr1_3:53563260-53563279 None:intergenic 25.0%
!! CTTTACATTTGGTGAAATAT+GGG - chr1_3:53563261-53563280 None:intergenic 25.0%
!! AATAACTTGTGCTTTACATT+TGG - chr1_3:53563272-53563291 None:intergenic 25.0%
! TCGATTGAAAATGAAAATGT+TGG + chr1_3:53563406-53563425 Msa0125190:intron 25.0%
! CGATTGAAAATGAAAATGTT+GGG + chr1_3:53563407-53563426 Msa0125190:intron 25.0%
!! ATGGGTATTGTTATAATTCA+GGG + chr1_3:53563520-53563539 Msa0125190:intron 25.0%
! ACAAATATCTCCATAAATGT+AGG - chr1_3:53563770-53563789 None:intergenic 25.0%
! GTGGGAAAAATACAAATTTA+TGG + chr1_3:53563965-53563984 Msa0125190:intron 25.0%
!! TTTACATTTGGTGAAATATG+GGG - chr1_3:53563260-53563279 None:intergenic 25.0%
!! CTTTACATTTGGTGAAATAT+GGG - chr1_3:53563261-53563280 None:intergenic 25.0%
!! AATAACTTGTGCTTTACATT+TGG - chr1_3:53563272-53563291 None:intergenic 25.0%
! TCGATTGAAAATGAAAATGT+TGG + chr1_3:53563406-53563425 Msa0125190:intron 25.0%
! CGATTGAAAATGAAAATGTT+GGG + chr1_3:53563407-53563426 Msa0125190:intron 25.0%
!! ATGGGTATTGTTATAATTCA+GGG + chr1_3:53563520-53563539 Msa0125190:intron 25.0%
! ACAAATATCTCCATAAATGT+AGG - chr1_3:53563770-53563789 None:intergenic 25.0%
! GTGGGAAAAATACAAATTTA+TGG + chr1_3:53563965-53563984 Msa0125190:intron 25.0%
TTTCAACAGAAACTCAAGTT+TGG + chr1_3:53563027-53563046 Msa0125190:CDS 30.0%
!!! CAGATGTTTTTTCAATGTCA+AGG - chr1_3:53563169-53563188 None:intergenic 30.0%
AATCTTTCAAACCAAACTTC+AGG + chr1_3:53563200-53563219 Msa0125190:CDS 30.0%
! GCTTTACATTTGGTGAAATA+TGG - chr1_3:53563262-53563281 None:intergenic 30.0%
!! ATTTTTCCTTTTCTAGTGCA+TGG + chr1_3:53563318-53563337 Msa0125190:intron 30.0%
!! TTTTTCCTTTTCTAGTGCAT+GGG + chr1_3:53563319-53563338 Msa0125190:intron 30.0%
ACTCATAAGTAGTGTAGAAT+GGG + chr1_3:53563434-53563453 Msa0125190:intron 30.0%
!! TTTTTAAGGGGTACAATTCA+TGG + chr1_3:53563501-53563520 Msa0125190:intron 30.0%
!! TTTTAAGGGGTACAATTCAT+GGG + chr1_3:53563502-53563521 Msa0125190:intron 30.0%
! CATGGGTATTGTTATAATTC+AGG + chr1_3:53563519-53563538 Msa0125190:intron 30.0%
! TGGGTATTGTTATAATTCAG+GGG + chr1_3:53563521-53563540 Msa0125190:intron 30.0%
!! ATTTTAAGTAGACACAGCAT+TGG + chr1_3:53563689-53563708 Msa0125190:intron 30.0%
ACTCTATATGCCTACATTTA+TGG + chr1_3:53563757-53563776 Msa0125190:intron 30.0%
AATGACAACTAAAGAAGAGT+TGG + chr1_3:53563798-53563817 Msa0125190:intron 30.0%
CTAGCTAATGTGTAACTATA+AGG - chr1_3:53563849-53563868 None:intergenic 30.0%
CACATTAGCTAGTATTCTTA+TGG + chr1_3:53563857-53563876 Msa0125190:intron 30.0%
!!! CATTAGTACCTTATTTTCTC+AGG + chr1_3:53563943-53563962 Msa0125190:intron 30.0%
!!! ACAAATGAACGCTTTTTTTG+AGG - chr1_3:53564003-53564022 None:intergenic 30.0%
TCAAACAACAAAATATCGTC+AGG - chr1_3:53564162-53564181 None:intergenic 30.0%
TTTATACCAAGCAATGAGAT+AGG + chr1_3:53564195-53564214 Msa0125190:CDS 30.0%
TTTCAACAGAAACTCAAGTT+TGG + chr1_3:53563027-53563046 Msa0125190:CDS 30.0%
!!! CAGATGTTTTTTCAATGTCA+AGG - chr1_3:53563169-53563188 None:intergenic 30.0%
AATCTTTCAAACCAAACTTC+AGG + chr1_3:53563200-53563219 Msa0125190:CDS 30.0%
! GCTTTACATTTGGTGAAATA+TGG - chr1_3:53563262-53563281 None:intergenic 30.0%
!! ATTTTTCCTTTTCTAGTGCA+TGG + chr1_3:53563318-53563337 Msa0125190:intron 30.0%
!! TTTTTCCTTTTCTAGTGCAT+GGG + chr1_3:53563319-53563338 Msa0125190:intron 30.0%
ACTCATAAGTAGTGTAGAAT+GGG + chr1_3:53563434-53563453 Msa0125190:intron 30.0%
!! TTTTTAAGGGGTACAATTCA+TGG + chr1_3:53563501-53563520 Msa0125190:intron 30.0%
!! TTTTAAGGGGTACAATTCAT+GGG + chr1_3:53563502-53563521 Msa0125190:intron 30.0%
! CATGGGTATTGTTATAATTC+AGG + chr1_3:53563519-53563538 Msa0125190:intron 30.0%
! TGGGTATTGTTATAATTCAG+GGG + chr1_3:53563521-53563540 Msa0125190:intron 30.0%
!! ATTTTAAGTAGACACAGCAT+TGG + chr1_3:53563689-53563708 Msa0125190:intron 30.0%
ACTCTATATGCCTACATTTA+TGG + chr1_3:53563757-53563776 Msa0125190:intron 30.0%
AATGACAACTAAAGAAGAGT+TGG + chr1_3:53563798-53563817 Msa0125190:intron 30.0%
CTAGCTAATGTGTAACTATA+AGG - chr1_3:53563849-53563868 None:intergenic 30.0%
CACATTAGCTAGTATTCTTA+TGG + chr1_3:53563857-53563876 Msa0125190:intron 30.0%
!!! CATTAGTACCTTATTTTCTC+AGG + chr1_3:53563943-53563962 Msa0125190:intron 30.0%
!!! ACAAATGAACGCTTTTTTTG+AGG - chr1_3:53564003-53564022 None:intergenic 30.0%
TCAAACAACAAAATATCGTC+AGG - chr1_3:53564162-53564181 None:intergenic 30.0%
TTTATACCAAGCAATGAGAT+AGG + chr1_3:53564195-53564214 Msa0125190:CDS 30.0%
TAAAACCCATGCACTAGAAA+AGG - chr1_3:53563327-53563346 None:intergenic 35.0%
GACTCATAAGTAGTGTAGAA+TGG + chr1_3:53563433-53563452 Msa0125190:intron 35.0%
! TCGTTGCAATGTGGATTAAA+GGG + chr1_3:53563639-53563658 Msa0125190:intron 35.0%
!!! TAGTACCTTATTTTCTCAGG+TGG + chr1_3:53563946-53563965 Msa0125190:intron 35.0%
!!! AGTACCTTATTTTCTCAGGT+GGG + chr1_3:53563947-53563966 Msa0125190:intron 35.0%
! TTTTCCCACCTGAGAAAATA+AGG - chr1_3:53563954-53563973 None:intergenic 35.0%
!! AAAAGCGTTCATTTGTAGAG+AGG + chr1_3:53564007-53564026 Msa0125190:intron 35.0%
! TTTTGTGGAAACTCATCCAT+TGG - chr1_3:53564093-53564112 None:intergenic 35.0%
! TATAGACTGAGAAGCTTTTG+TGG - chr1_3:53564108-53564127 None:intergenic 35.0%
GATACTTCTACAGATCAAGA+TGG + chr1_3:53564246-53564265 Msa0125190:CDS 35.0%
TAAAACCCATGCACTAGAAA+AGG - chr1_3:53563327-53563346 None:intergenic 35.0%
GACTCATAAGTAGTGTAGAA+TGG + chr1_3:53563433-53563452 Msa0125190:intron 35.0%
! TCGTTGCAATGTGGATTAAA+GGG + chr1_3:53563639-53563658 Msa0125190:intron 35.0%
!!! TAGTACCTTATTTTCTCAGG+TGG + chr1_3:53563946-53563965 Msa0125190:intron 35.0%
!!! AGTACCTTATTTTCTCAGGT+GGG + chr1_3:53563947-53563966 Msa0125190:intron 35.0%
! TTTTCCCACCTGAGAAAATA+AGG - chr1_3:53563954-53563973 None:intergenic 35.0%
!! AAAAGCGTTCATTTGTAGAG+AGG + chr1_3:53564007-53564026 Msa0125190:intron 35.0%
! TTTTGTGGAAACTCATCCAT+TGG - chr1_3:53564093-53564112 None:intergenic 35.0%
! TATAGACTGAGAAGCTTTTG+TGG - chr1_3:53564108-53564127 None:intergenic 35.0%
GATACTTCTACAGATCAAGA+TGG + chr1_3:53564246-53564265 Msa0125190:CDS 35.0%
CCACAAGCGTTACACAATAT+TGG - chr1_3:53563074-53563093 None:intergenic 40.0%
CCAATATTGTGTAACGCTTG+TGG + chr1_3:53563071-53563090 Msa0125190:CDS 40.0%
CGCTTGTGGTTTACATTTCA+AGG + chr1_3:53563085-53563104 Msa0125190:CDS 40.0%
GGTTTACATTTCAAGGCGAA+AGG + chr1_3:53563092-53563111 Msa0125190:CDS 40.0%
!! GTGCATGGGTTTTAATGTAG+CGG + chr1_3:53563333-53563352 Msa0125190:intron 40.0%
ATAAGTAGTGTAGAATGGGC+AGG + chr1_3:53563438-53563457 Msa0125190:intron 40.0%
! ACGCTGCTAAAGTTTTCTAC+GGG - chr1_3:53563566-53563585 None:intergenic 40.0%
! CACGCTGCTAAAGTTTTCTA+CGG - chr1_3:53563567-53563586 None:intergenic 40.0%
! GTCGTTGCAATGTGGATTAA+AGG + chr1_3:53563638-53563657 Msa0125190:intron 40.0%
!! TGTGGATTAAAGGGTGTTTG+AGG + chr1_3:53563648-53563667 Msa0125190:intron 40.0%
!! GTGGATTAAAGGGTGTTTGA+GGG + chr1_3:53563649-53563668 Msa0125190:intron 40.0%
!! ATTGGTGTCGTCATCTCTAT+TGG - chr1_3:53564057-53564076 None:intergenic 40.0%
TCTTGGATGCATTCGTCTAA+AGG + chr1_3:53564218-53564237 Msa0125190:CDS 40.0%
CCACAAGCGTTACACAATAT+TGG - chr1_3:53563074-53563093 None:intergenic 40.0%
CCAATATTGTGTAACGCTTG+TGG + chr1_3:53563071-53563090 Msa0125190:CDS 40.0%
CGCTTGTGGTTTACATTTCA+AGG + chr1_3:53563085-53563104 Msa0125190:CDS 40.0%
GGTTTACATTTCAAGGCGAA+AGG + chr1_3:53563092-53563111 Msa0125190:CDS 40.0%
!! GTGCATGGGTTTTAATGTAG+CGG + chr1_3:53563333-53563352 Msa0125190:intron 40.0%
ATAAGTAGTGTAGAATGGGC+AGG + chr1_3:53563438-53563457 Msa0125190:intron 40.0%
! ACGCTGCTAAAGTTTTCTAC+GGG - chr1_3:53563566-53563585 None:intergenic 40.0%
! CACGCTGCTAAAGTTTTCTA+CGG - chr1_3:53563567-53563586 None:intergenic 40.0%
! GTCGTTGCAATGTGGATTAA+AGG + chr1_3:53563638-53563657 Msa0125190:intron 40.0%
!! TGTGGATTAAAGGGTGTTTG+AGG + chr1_3:53563648-53563667 Msa0125190:intron 40.0%
!! GTGGATTAAAGGGTGTTTGA+GGG + chr1_3:53563649-53563668 Msa0125190:intron 40.0%
!! ATTGGTGTCGTCATCTCTAT+TGG - chr1_3:53564057-53564076 None:intergenic 40.0%
TCTTGGATGCATTCGTCTAA+AGG + chr1_3:53564218-53564237 Msa0125190:CDS 40.0%
GAAACTCAAGTTTGGCGCAA+AGG + chr1_3:53563035-53563054 Msa0125190:CDS 45.0%
CACAATATTGGCGTCTCGTA+TGG - chr1_3:53563062-53563081 None:intergenic 45.0%
CTTCGGGAGATAGTTAGCAA+GGG - chr1_3:53563120-53563139 None:intergenic 45.0%
TCTTCGGGAGATAGTTAGCA+AGG - chr1_3:53563121-53563140 None:intergenic 45.0%
! TGATTTTGCAGGGCTCTCTT+CGG - chr1_3:53563137-53563156 None:intergenic 45.0%
AGCCCTGCAAAATCAGTCAT+CGG + chr1_3:53563142-53563161 Msa0125190:CDS 45.0%
!! GTCCGATGACTGATTTTGCA+GGG - chr1_3:53563147-53563166 None:intergenic 45.0%
! CGCTGCTAAAGTTTTCTACG+GGG - chr1_3:53563565-53563584 None:intergenic 45.0%
TGAGAGTTGTCGTTGCAATG+TGG + chr1_3:53563630-53563649 Msa0125190:intron 45.0%
GGAAACTCATCCATTGGTGT+CGG - chr1_3:53564087-53564106 None:intergenic 45.0%
CCAAGACCTATCTCATTGCT+TGG - chr1_3:53564204-53564223 None:intergenic 45.0%
CCAAGCAATGAGATAGGTCT+TGG + chr1_3:53564201-53564220 Msa0125190:CDS 45.0%
GAAACTCAAGTTTGGCGCAA+AGG + chr1_3:53563035-53563054 Msa0125190:CDS 45.0%
CACAATATTGGCGTCTCGTA+TGG - chr1_3:53563062-53563081 None:intergenic 45.0%
CTTCGGGAGATAGTTAGCAA+GGG - chr1_3:53563120-53563139 None:intergenic 45.0%
TCTTCGGGAGATAGTTAGCA+AGG - chr1_3:53563121-53563140 None:intergenic 45.0%
! TGATTTTGCAGGGCTCTCTT+CGG - chr1_3:53563137-53563156 None:intergenic 45.0%
AGCCCTGCAAAATCAGTCAT+CGG + chr1_3:53563142-53563161 Msa0125190:CDS 45.0%
!! GTCCGATGACTGATTTTGCA+GGG - chr1_3:53563147-53563166 None:intergenic 45.0%
! CGCTGCTAAAGTTTTCTACG+GGG - chr1_3:53563565-53563584 None:intergenic 45.0%
TGAGAGTTGTCGTTGCAATG+TGG + chr1_3:53563630-53563649 Msa0125190:intron 45.0%
GGAAACTCATCCATTGGTGT+CGG - chr1_3:53564087-53564106 None:intergenic 45.0%
CCAAGACCTATCTCATTGCT+TGG - chr1_3:53564204-53564223 None:intergenic 45.0%
CCAAGCAATGAGATAGGTCT+TGG + chr1_3:53564201-53564220 Msa0125190:CDS 45.0%
! GATTTTGCAGGGCTCTCTTC+GGG - chr1_3:53563136-53563155 None:intergenic 50.0%
!! GGTCCGATGACTGATTTTGC+AGG - chr1_3:53563148-53563167 None:intergenic 50.0%
!! ATTGGTGTCGGCGTCTCTAT+TGG - chr1_3:53564075-53564094 None:intergenic 50.0%
AATAGAGACGCCGACACCAA+TGG + chr1_3:53564074-53564093 Msa0125190:CDS 50.0%
! GATTTTGCAGGGCTCTCTTC+GGG - chr1_3:53563136-53563155 None:intergenic 50.0%
!! GGTCCGATGACTGATTTTGC+AGG - chr1_3:53563148-53563167 None:intergenic 50.0%
!! ATTGGTGTCGGCGTCTCTAT+TGG - chr1_3:53564075-53564094 None:intergenic 50.0%
AATAGAGACGCCGACACCAA+TGG + chr1_3:53564074-53564093 Msa0125190:CDS 50.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 53563023 53564272 53563023 ID=Msa0125190;Name=Msa0125190
chr1_3 mRNA 53563023 53564272 53563023 ID=Msa0125190-mRNA-1;Parent=Msa0125190;Name=Msa0125190-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0|0|1|0|0.5|2|0|148
chr1_3 exon 53563023 53563221 53563023 ID=Msa0125190-mRNA-1:exon:16382;Parent=Msa0125190-mRNA-1
chr1_3 exon 53564025 53564272 53564025 ID=Msa0125190-mRNA-1:exon:16383;Parent=Msa0125190-mRNA-1
chr1_3 CDS 53563023 53563221 53563023 ID=Msa0125190-mRNA-1:cds;Parent=Msa0125190-mRNA-1
chr1_3 CDS 53564025 53564272 53564025 ID=Msa0125190-mRNA-1:cds;Parent=Msa0125190-mRNA-1
Gene Sequence

>Msa0125190

ATGGTTTCAACAGAAACTCAAGTTTGGCGCAAAGGACCATACGAGACGCCAATATTGTGTAACGCTTGTGGTTTACATTTCAAGGCGAAAGGAACCCTTGCTAACTATCTCCCGAAGAGAGCCCTGCAAAATCAGTCATCGGACCTTGACATTGAAAAAACATCTGAAAGTAATGCAAATCTTTCAAACCAAACTTCAGAGAGGCATTCATCGACTGTGATGCAGACACCAATAGAGATGACGACACCAATAGAGACGCCGACACCAATGGATGAGTTTCCACAAAAGCTTCTCAGTCTATATGAATCTGAGAAAGAAGAAAATGAATCATCTCCTGACGATATTTTGTTGTTTGAGAAAATGAACAACTTTATACCAAGCAATGAGATAGGTCTTGGATGCATTCGTCTAAAGGCAGATGATACTTCTACAGATCAAGATGGTTAA

Protein sequence

>Msa0125190

MVSTETQVWRKGPYETPILCNACGLHFKAKGTLANYLPKRALQNQSSDLDIEKTSESNANLSNQTSERHSSTVMQTPIEMTTPIETPTPMDEFPQKLLSLYESEKEENESSPDDILLFEKMNNFIPSNEIGLGCIRLKADDTSTDQDG*