AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0127550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0127550 MtrunA17_Chr1g0191141 85.586 1110 141 5 28 1131 7 1103 0.0 1942
Msa0127550 MtrunA17_Chr7g0224051 66.548 1127 315 16 55 1130 1 1116 0.0 1449
Msa0127550 MtrunA17_Chr3g0114161 72.196 856 210 10 300 1130 395 1247 0.0 1208
Msa0127550 MtrunA17_Chr3g0114161 64.746 295 90 4 28 308 24 318 6.25e-111 375
Msa0127550 MtrunA17_Chr5g0437621 59.719 854 309 17 305 1130 457 1303 0.0 961
Msa0127550 MtrunA17_Chr5g0437621 43.421 304 135 7 29 307 19 310 2.34e-55 211
Msa0127550 MtrunA17_Chr7g0225791 34.813 721 426 16 406 1111 577 1268 2.20e-115 388
Msa0127550 MtrunA17_Chr7g0233031 34.345 725 404 16 424 1117 549 1232 1.57e-114 385
Msa0127550 MtrunA17_Chr7g0233021 35.784 721 386 18 424 1111 537 1213 5.80e-114 383
Msa0127550 MtrunA17_Chr4g0047081 34.722 720 409 17 424 1121 548 1228 1.66e-112 379
Msa0127550 MtrunA17_Chr7g0232971 35.085 761 403 22 406 1126 584 1293 2.82e-112 380
Msa0127550 MtrunA17_Chr7g0224031 68.493 219 68 1 693 910 24 242 2.27e-96 306
Msa0127550 MtrunA17_Chr2g0296421 31.664 679 378 16 419 1086 398 1001 4.40e-91 315
Msa0127550 MtrunA17_Chr7g0232991 33.280 628 342 16 532 1117 54 646 6.38e-89 301
Msa0127550 MtrunA17_Chr8g0352361 31.143 700 387 19 402 1086 233 852 1.20e-88 306
Msa0127550 MtrunA17_Chr7g0233001 33.764 542 307 13 601 1121 3 513 8.27e-75 257
Msa0127550 MtrunA17_Chr7g0224021 70.536 112 31 1 521 632 2 111 1.56e-47 165
Msa0127550 MtrunA17_Chr2g0320781 37.247 247 139 7 839 1079 4 240 4.97e-39 145
Msa0127550 MtrunA17_Chr8g0384531 25.087 578 327 21 538 1078 372 880 1.44e-29 127
Msa0127550 MtrunA17_Chr5g0393461 24.132 634 371 23 513 1116 163 716 2.52e-29 126
Msa0127550 MtrunA17_Chr5g0403921 24.821 560 329 19 585 1129 206 688 1.47e-23 107
Msa0127550 MtrunA17_Chr1g0190391 27.731 238 144 6 544 777 576 789 6.41e-22 102
Msa0127550 MtrunA17_Chr3g0137091 25.050 499 296 16 584 1069 310 743 3.37e-20 97.4
Msa0127550 MtrunA17_Chr1g0190381 35.526 152 88 3 949 1091 205 355 1.04e-18 91.3
Msa0127550 MtrunA17_Chr5g0394201 23.346 544 329 20 585 1118 385 850 1.28e-15 82.4
Msa0127550 MtrunA17_Chr5g0442141 22.374 514 309 18 577 1075 633 1071 8.35e-15 80.1
Msa0127550 MtrunA17_Chr3g0095091 24.376 521 296 21 577 1078 587 1028 1.20e-14 79.3
Msa0127550 MtrunA17_Chr4g0066741 23.017 517 299 21 585 1088 210 640 4.29e-14 77.4
Msa0127550 MtrunA17_Chr4g0040301 31.138 167 102 4 941 1100 389 549 7.42e-14 76.3
Msa0127550 MtrunA17_Chr2g0286291 23.183 509 308 18 585 1088 213 643 1.59e-13 75.5
Msa0127550 MtrunA17_Chr3g0114111 88.235 34 4 0 1084 1117 1 34 1.75e-12 63.9
Msa0127550 MtrunA17_Chr7g0257101 29.545 176 116 5 913 1087 1173 1341 6.24e-12 70.5
Msa0127550 MtrunA17_Chr7g0255821 39.362 94 56 1 977 1069 690 783 3.67e-11 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0127550 AT3G42670.2 57.716 797 299 12 360 1131 472 1255 0.0 896
Msa0127550 AT3G42670.2 37.102 283 150 7 29 298 18 285 2.87e-42 169
Msa0127550 AT3G42670.3 57.716 797 299 12 360 1131 472 1255 0.0 896
Msa0127550 AT3G42670.3 37.102 283 150 7 29 298 18 285 2.87e-42 169
Msa0127550 AT3G42670.4 57.716 797 301 12 360 1131 472 1257 0.0 896
Msa0127550 AT3G42670.4 37.102 283 150 7 29 298 18 285 2.90e-42 169
Msa0127550 AT3G42670.1 57.716 797 299 12 360 1131 472 1255 0.0 896
Msa0127550 AT3G42670.1 37.102 283 150 7 29 298 18 285 2.87e-42 169
Msa0127550 AT5G20420.1 55.542 821 329 10 339 1131 449 1261 0.0 882
Msa0127550 AT5G20420.1 34.448 299 149 6 29 302 18 294 3.26e-42 169
Msa0127550 AT1G05490.1 32.964 722 422 18 424 1115 695 1384 7.33e-105 360
Msa0127550 AT3G24340.1 32.222 720 421 15 413 1119 449 1114 5.20e-102 348
Msa0127550 AT2G16390.2 31.909 702 384 19 402 1086 226 850 1.01e-87 303
Msa0127550 AT2G16390.1 31.909 702 384 19 402 1086 223 847 1.18e-87 303
Msa0127550 AT2G21450.3 32.209 652 357 20 459 1086 92 682 1.56e-84 290
Msa0127550 AT2G21450.2 31.748 652 360 19 459 1086 190 780 3.43e-84 292
Msa0127550 AT2G21450.1 31.748 652 360 19 459 1086 183 773 5.47e-84 291
Msa0127550 AT3G19210.2 23.729 590 356 21 550 1110 180 704 3.61e-27 120
Msa0127550 AT3G19210.1 24.407 590 354 22 550 1110 180 706 5.65e-27 119
Msa0127550 AT3G19210.3 24.407 590 354 22 550 1110 180 706 5.89e-27 119
Msa0127550 AT5G63950.2 24.417 557 315 23 550 1078 377 855 1.18e-24 112
Msa0127550 AT5G63950.1 24.643 560 327 23 550 1078 377 872 1.24e-24 112
Msa0127550 AT1G08600.4 26.866 335 189 12 550 875 719 1006 8.40e-24 109
Msa0127550 AT1G08600.4 34.969 163 94 4 938 1091 1123 1282 1.23e-17 89.7
Msa0127550 AT1G08600.3 26.866 335 189 12 550 875 719 1006 8.40e-24 109
Msa0127550 AT1G08600.3 34.969 163 94 4 938 1091 1123 1282 1.23e-17 89.7
Msa0127550 AT1G08600.2 26.866 335 189 12 550 875 719 1006 8.40e-24 109
Msa0127550 AT1G08600.2 34.969 163 94 4 938 1091 1123 1282 1.23e-17 89.7
Msa0127550 AT5G44800.1 25.992 504 285 21 581 1069 707 1137 2.78e-21 101
Msa0127550 AT4G31900.3 23.801 542 302 19 540 1069 214 656 5.36e-20 97.1
Msa0127550 AT4G31900.1 23.801 542 302 19 540 1069 214 656 7.25e-20 96.7
Msa0127550 AT4G31900.2 23.801 542 302 19 540 1069 173 615 7.75e-20 96.7
Msa0127550 AT1G08600.1 34.969 163 94 4 938 1091 1102 1261 1.20e-17 89.7
Msa0127550 AT1G08600.1 24.024 333 180 9 550 875 719 985 6.58e-17 87.0
Msa0127550 AT2G13370.1 23.092 511 301 18 584 1078 646 1080 3.40e-15 81.6
Msa0127550 AT2G13370.2 23.092 511 301 18 584 1078 646 1080 3.40e-15 81.6
Msa0127550 AT2G13370.3 23.092 511 301 18 584 1078 646 1080 3.40e-15 81.6
Msa0127550 AT1G03750.1 30.682 176 108 5 933 1100 528 697 7.17e-15 80.1
Msa0127550 AT2G44980.1 25.097 518 298 22 591 1092 80 523 5.74e-13 73.9
Msa0127550 AT3G57300.1 30.114 176 115 5 913 1087 1187 1355 7.78e-13 73.9
Msa0127550 AT3G57300.4 30.114 176 115 5 913 1087 1187 1355 7.78e-13 73.9
Msa0127550 AT3G57300.2 30.114 176 115 5 913 1087 1220 1388 9.50e-13 73.6
Msa0127550 AT2G18760.4 31.387 137 88 3 950 1086 745 875 1.00e-11 70.1
Msa0127550 AT2G18760.1 31.387 137 88 3 950 1086 745 875 1.00e-11 70.1
Msa0127550 AT2G18760.2 31.387 137 88 3 950 1086 745 875 1.00e-11 70.1
Msa0127550 AT2G18760.7 31.387 137 88 3 950 1086 745 875 1.00e-11 70.1
Msa0127550 AT2G18760.5 31.387 137 88 3 950 1086 745 875 1.00e-11 70.1
Msa0127550 AT2G18760.6 31.387 137 88 3 950 1086 745 875 1.00e-11 70.1
Msa0127550 AT2G18760.3 31.387 137 88 3 950 1086 804 934 1.07e-11 70.1
Msa0127550 AT5G66750.1 29.940 167 112 2 950 1116 542 703 3.93e-11 67.8

Find 168 sgRNAs with CRISPR-Local

Find 254 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTTGTTTCAAAACAATTT+TGG 0.098097 1_3:+56958475 Msa0127550:CDS
TAAGAGAAGGTGGTTGTTTA+AGG 0.177258 1_3:+56956568 Msa0127550:CDS
CCAGGCAGAACCATTGATTT+TGG 0.203037 1_3:-56958169 None:intergenic
AGTCCTCCAATTTCGCTTCT+TGG 0.213233 1_3:-56956425 None:intergenic
AGAACTTCGTGAATAAATTT+TGG 0.216798 1_3:-56958529 None:intergenic
GATGCTTACATGGATAATTT+TGG 0.217962 1_3:+56956737 Msa0127550:CDS
TGCTTGACACCATTAGAATT+TGG 0.218550 1_3:-56958196 None:intergenic
AACTTTGATGTGGTACTTCT+AGG 0.221583 1_3:-56958400 None:intergenic
CTTATCTTCTGTTCTCATTA+AGG 0.232667 1_3:-56958299 None:intergenic
TATCGATTGGATGAACAAAT+TGG 0.242680 1_3:+56957311 Msa0127550:CDS
ATATGGATTTGATAGCTTTA+TGG 0.245952 1_3:+56956594 Msa0127550:CDS
TCGACCTGGTCAAGAGAAAA+TGG 0.262902 1_3:+56959353 Msa0127550:CDS
ACATCAAGTTATCTTCTTGA+TGG 0.278990 1_3:+56956896 Msa0127550:CDS
TCACTGATAGTTGGATCTTC+TGG 0.280474 1_3:-56956767 None:intergenic
AGTTATTTCGTTGAAGTCTT+TGG 0.292757 1_3:-56956091 None:intergenic
GGTAGGTGACAAGGCTAAAA+TGG 0.298106 1_3:+56957681 Msa0127550:intron
TAATGGTGTCAAGCATGATT+TGG 0.304673 1_3:+56958204 Msa0127550:CDS
TATGGATTTGATAGCTTTAT+GGG 0.316460 1_3:+56956595 Msa0127550:CDS
CCAATGCCAAGAAGCGAAAT+TGG 0.318140 1_3:+56956419 Msa0127550:CDS
CCAAAGAAAATGATAGTGTT+TGG 0.318198 1_3:+56957807 Msa0127550:CDS
TTGAAGTTCAAAACATTCAT+AGG 0.322383 1_3:-56956344 None:intergenic
CAAGCTTGTGCATCAAGTTA+TGG 0.328101 1_3:+56956298 Msa0127550:CDS
CCAATTTCGCTTCTTGGCAT+TGG 0.336402 1_3:-56956419 None:intergenic
AACAAATTTGCTTTGAGAAA+AGG 0.336871 1_3:+56958982 Msa0127550:CDS
TTGGTGTCGTTGAATGTATC+TGG 0.339974 1_3:-56958150 None:intergenic
ATACGATAATAGCGCTATAA+TGG 0.343852 1_3:+56955226 Msa0127550:CDS
AGTTCTTCTGATACTCTTCC+TGG 0.348560 1_3:+56958748 Msa0127550:CDS
AAAGTTGAGGAAGTGCTTAA+TGG 0.350370 1_3:+56958417 Msa0127550:CDS
GCAGAAACTGCAAAAGAAAA+TGG 0.357505 1_3:+56958825 Msa0127550:CDS
GCTCTTCATTGCCACAATCT+AGG 0.360216 1_3:+56956183 Msa0127550:CDS
AAAATTTATTCACGAAGTTC+TGG 0.379765 1_3:+56958531 Msa0127550:CDS
TTCATAGGACTACTAATATT+AGG 0.382189 1_3:-56956329 None:intergenic
GTGGTTGTTTAAGGAGCATA+TGG 0.383291 1_3:+56956577 Msa0127550:CDS
TGTCTTTCTTCACTGATAGT+TGG 0.384151 1_3:-56956776 None:intergenic
ATATGTCAATTCCAGGTCTT+AGG 0.387141 1_3:-56955725 None:intergenic
AAGCTTGTGCATCAAGTTAT+GGG 0.390339 1_3:+56956299 Msa0127550:CDS
AGGTGGTGATAATGTTTCTT+CGG 0.390951 1_3:+56956514 Msa0127550:CDS
CATGTGTAGAGTGTGGTCTT+TGG 0.395080 1_3:-56958064 None:intergenic
GCTCAAAACAAATCTTACTT+TGG 0.400942 1_3:-56959007 None:intergenic
GGTGGTGATAATGTTTCTTC+GGG 0.402375 1_3:+56956515 Msa0127550:CDS
AATTCCAGGTCTTAGGAAAC+TGG 0.403179 1_3:-56955718 None:intergenic
CCTGGAGTTCCAAATTCTAA+TGG 0.403216 1_3:+56958187 Msa0127550:CDS
AAGAAAATGGCGGGCTGTAC+CGG 0.405933 1_3:+56958838 Msa0127550:CDS
CAAAGAAAATGATAGTGTTT+GGG 0.407919 1_3:+56957808 Msa0127550:CDS
GGTAGAAAATCCTGATGATT+TGG 0.409165 1_3:+56956841 Msa0127550:CDS
GTCTTTGGAGCTAGGACTAA+TGG 0.409580 1_3:-56958049 None:intergenic
AAAGAGATTCTGCAACTAAC+TGG 0.420585 1_3:+56959141 Msa0127550:CDS
CTCAACCTCAAGAGAGTAAC+CGG 0.423138 1_3:-56958857 None:intergenic
ATCATGTTCACATGTTTCCT+TGG 0.426716 1_3:-56957288 None:intergenic
CACCCTAGTGTTCTTGTCAT+GGG 0.427375 1_3:+56958259 Msa0127550:CDS
TCAACTGATGCTACTGATCT+TGG 0.430086 1_3:+56957773 Msa0127550:CDS
ACGCGCGAGAAAATTCTTCT+TGG 0.431056 1_3:+56958618 Msa0127550:CDS
AAGGAAGAGGATAATGTGAT+TGG 0.437162 1_3:+56956047 Msa0127550:CDS
TACGTAATGTTCAAGTTGTA+TGG 0.439233 1_3:-56956468 None:intergenic
AAGAAAAGAGATGATGAGAA+AGG 0.450749 1_3:-56957993 None:intergenic
ACACCCTAGTGTTCTTGTCA+TGG 0.450907 1_3:+56958258 Msa0127550:CDS
TCTATATTTATCTTCCTCCA+TGG 0.451446 1_3:-56959406 None:intergenic
TTGAACTTCAAATCAGAAAA+CGG 0.451497 1_3:+56956356 Msa0127550:CDS
CTGATGATTTGGCTGGCATA+TGG 0.454793 1_3:+56956852 Msa0127550:CDS
AGCACAGATATGTCAATTCC+AGG 0.458031 1_3:-56955732 None:intergenic
ATATCTCATGCTCCTGGAGC+TGG 0.462480 1_3:+56957965 Msa0127550:CDS
TACTATGAGTTTGTTGTGCA+TGG 0.462547 1_3:-56955641 None:intergenic
TCAATCAATGCTTGTTCTGA+AGG 0.465693 1_3:+56959243 Msa0127550:CDS
TATCGATGATCATGAAGAAC+AGG 0.466478 1_3:+56956664 Msa0127550:CDS
GCATTTGAAGCAGTATTGAA+TGG 0.467351 1_3:+56955558 Msa0127550:intron
AGTAAAATTCTCAATGCTGC+TGG 0.475556 1_3:-56958445 None:intergenic
GTATATCACTTGTTGATGAC+TGG 0.488667 1_3:+56959381 Msa0127550:CDS
AGAGTGTGGTCTTTGGAGCT+AGG 0.489103 1_3:-56958057 None:intergenic
CACAATCTCGTGTTGTTCAT+CGG 0.491623 1_3:-56958803 None:intergenic
CCATTAGAATTTGGAACTCC+AGG 0.495262 1_3:-56958187 None:intergenic
TACCGCGTCATCTTTAAAGA+AGG 0.495527 1_3:+56956247 Msa0127550:CDS
CATATGCCAGCCAAATCATC+AGG 0.497067 1_3:-56956851 None:intergenic
ACGTGTGATGAGCAAGGAAA+AGG 0.497284 1_3:+56959038 Msa0127550:CDS
AATCTTCTCAGCTTGCCATT+CGG 0.505551 1_3:-56959493 None:intergenic
TATTTCGTTGAAGTCTTTGG+AGG 0.508106 1_3:-56956088 None:intergenic
AGATTGTAATGATGAAAAGT+TGG 0.511319 1_3:+56956490 Msa0127550:CDS
GTAATTTGTCATCAGACATC+CGG 0.513031 1_3:+56955661 Msa0127550:CDS
GCGCATGGCTAAAGCATTGA+GGG 0.513248 1_3:+56958339 Msa0127550:CDS
AGCGCTATAATGGATATTCA+TGG 0.513605 1_3:+56955236 Msa0127550:CDS
TGCTCCAGTTTCCTAAGACC+TGG 0.515668 1_3:+56955714 Msa0127550:CDS
CATGAAATCGACATGAACAT+TGG 0.516088 1_3:-56955974 None:intergenic
CTTGTTGATGACTGGATCCA+TGG 0.517661 1_3:+56959389 Msa0127550:CDS
GAAGAACTACTTGGAAAGAG+TGG 0.517977 1_3:+56959427 Msa0127550:CDS
AAGCAGTATTGAATGGCTCA+TGG 0.520661 1_3:+56955565 Msa0127550:CDS
GAAACAATGCAGTGAAGATA+CGG 0.522442 1_3:+56957715 Msa0127550:CDS
CCAAAATCAATGGTTCTGCC+TGG 0.525654 1_3:+56958169 Msa0127550:CDS
TGTGTTATATCTCATGCTCC+TGG 0.525908 1_3:+56957959 Msa0127550:CDS
TTATCTTCTTGATGGAAATG+AGG 0.528274 1_3:+56956904 Msa0127550:CDS
TGGATAAGATACACCGGCAC+GGG 0.534854 1_3:-56958115 None:intergenic
GGTTGCATTTGTCATAAGAT+CGG 0.534985 1_3:+56956268 Msa0127550:CDS
TGAAGAAAGACAGTTTGAGC+AGG 0.538289 1_3:+56956787 Msa0127550:CDS
TATGATGATCAAGGTTCACT+TGG 0.538490 1_3:+56956005 Msa0127550:CDS
TTCAACGACACCAAAATCAA+TGG 0.539455 1_3:+56958159 Msa0127550:CDS
CTGTACCGGTTACTCTCTTG+AGG 0.542553 1_3:+56958852 Msa0127550:CDS
TGATGATTTGGCTGGCATAT+GGG 0.542896 1_3:+56956853 Msa0127550:CDS
TTCTCAGCTTGCCATTCGGA+AGG 0.544538 1_3:-56959489 None:intergenic
TTGTGCATGGAGGAAATGTA+TGG 0.544715 1_3:-56955628 None:intergenic
CGTATCGAAAAGCGGTGATG+AGG 0.549235 1_3:+56956820 Msa0127550:CDS
GAAAATCCTGATGATTTGGC+TGG 0.557990 1_3:+56956845 Msa0127550:CDS
GTCTAGAAAAGATCAAAAGC+TGG 0.560297 1_3:+56958230 Msa0127550:CDS
ATAAATATAGAAGAACTACT+TGG 0.563098 1_3:+56959418 Msa0127550:CDS
TTAGATTCTAAGTACAGAAG+AGG 0.564016 1_3:+56958559 Msa0127550:CDS
AGATTGAAGATGAAATACTC+AGG 0.564407 1_3:+56959511 Msa0127550:CDS
TGACACAAATAAAGAATCCA+AGG 0.564564 1_3:+56957271 Msa0127550:CDS
AATGTATAGATTTGCAAACC+AGG 0.566512 1_3:-56958766 None:intergenic
AAGAACTACTTGGAAAGAGT+GGG 0.567137 1_3:+56959428 Msa0127550:CDS
GCGCGTGCCTCTAGTAAATG+AGG 0.568077 1_3:-56958601 None:intergenic
TCATCAAGTATCAAGAGTCC+AGG 0.569191 1_3:-56958367 None:intergenic
TAACCCATGACAAGAACACT+AGG 0.569323 1_3:-56958262 None:intergenic
AGCGCATGGCTAAAGCATTG+AGG 0.574680 1_3:+56958338 Msa0127550:CDS
TGCTCATCACACGTGAGATT+AGG 0.575173 1_3:-56959029 None:intergenic
GAACATTGGTTATATTTGTG+AGG 0.579746 1_3:-56955960 None:intergenic
GGAAGTAATCGATGCTTACA+TGG 0.582633 1_3:+56956727 Msa0127550:CDS
AAACTGCAAAAGAAAATGGC+GGG 0.587027 1_3:+56958829 Msa0127550:CDS
ATGCCAAGAAGCGAAATTGG+AGG 0.587187 1_3:+56956422 Msa0127550:CDS
GTATCTGGCTTCGACAGCTG+TGG 0.588022 1_3:-56958135 None:intergenic
ATATCCAATGGTAGGTGACA+AGG 0.588447 1_3:+56957672 Msa0127550:intron
AGCAGTATTGAATGGCTCAT+GGG 0.590169 1_3:+56955566 Msa0127550:CDS
AAAGCATTGAGGGAAAGTCC+TGG 0.590693 1_3:+56958349 Msa0127550:CDS
GCACTTCCTCAACTTTGATG+TGG 0.590805 1_3:-56958410 None:intergenic
CCGAAAAGTAACCTAGATTG+TGG 0.592635 1_3:-56956194 None:intergenic
AGATGAAATACTCAGGGAGA+TGG 0.595348 1_3:+56959518 Msa0127550:CDS
CTTCAACGAAATAACTGTGA+AGG 0.595665 1_3:+56956098 Msa0127550:CDS
AGCATCGATTACTTCCTCAC+AGG 0.596566 1_3:-56956720 None:intergenic
CAAAGAAGCTTAAGAGAAGG+TGG 0.596830 1_3:+56956558 Msa0127550:CDS
AAAATGGAATATTCCCGTGC+CGG 0.598414 1_3:+56958102 Msa0127550:CDS
GATAAATTTGAAGATCGTTG+TGG 0.605781 1_3:+56959198 Msa0127550:CDS
TGATAGTTGGATCTTCTGGT+AGG 0.608458 1_3:-56956763 None:intergenic
TGGACTCTTGATACTTGATG+AGG 0.608858 1_3:+56958369 Msa0127550:CDS
GGAATATACTGCCGTACATG+CGG 0.610029 1_3:+56957332 Msa0127550:CDS
ACTCAGGGAGATGGTTGAAG+AGG 0.610175 1_3:+56959527 Msa0127550:CDS
AGATGACGCGGTAACAAACC+AGG 0.611437 1_3:-56956237 None:intergenic
GCAGGGTGCAAATGCTGATG+AGG 0.611917 1_3:+56957241 Msa0127550:intron
CAAAGACCACACTCTACACA+TGG 0.613075 1_3:+56958065 Msa0127550:CDS
CACTCGGGTCAATACACCCA+TGG 0.615844 1_3:+56958890 Msa0127550:CDS
CAGAAGAGGCAAACTAGAGG+AGG 0.618526 1_3:+56958573 Msa0127550:CDS
AAATTCACACAGAAAGCGCA+TGG 0.619203 1_3:+56958324 Msa0127550:CDS
GAGCATGATTATGACGGAGA+AGG 0.621402 1_3:+56956386 Msa0127550:CDS
AATCTCACGTGTGATGAGCA+AGG 0.622148 1_3:+56959032 Msa0127550:CDS
TCACTTGAGCATGATTATGA+CGG 0.623465 1_3:+56956380 Msa0127550:CDS
GATTGAAGATGAAATACTCA+GGG 0.623647 1_3:+56959512 Msa0127550:CDS
CATGAAGAACAGGATCATAG+AGG 0.626014 1_3:+56956674 Msa0127550:CDS
GAAGCGATACAATCCAAAGA+AGG 0.629502 1_3:+56956617 Msa0127550:CDS
AATGAACTCAAATATTGATG+CGG 0.633014 1_3:+56958657 Msa0127550:CDS
ATGTGAACATGATTATCGAT+TGG 0.633134 1_3:+56957298 Msa0127550:CDS
GAAACTGCAAAAGAAAATGG+CGG 0.634136 1_3:+56958828 Msa0127550:CDS
GTACAGAAGAGGCAAACTAG+AGG 0.636952 1_3:+56958570 Msa0127550:CDS
GGACTCTTGATACTTGATGA+GGG 0.637751 1_3:+56958370 Msa0127550:CDS
GCACAAAGAAGCTTAAGAGA+AGG 0.639100 1_3:+56956555 Msa0127550:CDS
GAATGGCTCATGGGAACCTG+TGG 0.639123 1_3:+56955575 Msa0127550:CDS
AAAGAGAAATCTGACACAAG+TGG 0.641751 1_3:+56957932 Msa0127550:CDS
GTCACAACCTTGCACCAGTG+AGG 0.646068 1_3:+56959073 Msa0127550:CDS
TGTAATGATGAAAAGTTGGA+AGG 0.659731 1_3:+56956494 Msa0127550:CDS
GAAGTACCACATCAAAGTTG+AGG 0.659873 1_3:+56958404 Msa0127550:CDS
GTGGATAAGATACACCGGCA+CGG 0.666067 1_3:-56958116 None:intergenic
TTGTTGAAGATCCTTCCGAA+TGG 0.668864 1_3:+56959478 Msa0127550:CDS
AACCTTCTTTAAAGATGACG+CGG 0.674091 1_3:-56956249 None:intergenic
AAAGAAAACGTATCGAAAAG+CGG 0.683079 1_3:+56956812 Msa0127550:CDS
GACTACGACGCTCAAAACGT+AGG 0.683290 1_3:+56956444 Msa0127550:CDS
CAGCTGTGGATAAGATACAC+CGG 0.684401 1_3:-56958121 None:intergenic
GAGAAATCTGACACAAGTGG+TGG 0.685073 1_3:+56957935 Msa0127550:CDS
AACCCATGACAAGAACACTA+GGG 0.686530 1_3:-56958261 None:intergenic
AATGATGAAAAGTTGGAAGG+TGG 0.696462 1_3:+56956497 Msa0127550:CDS
GGAAGCGCCTCATTTACTAG+AGG 0.697989 1_3:+56958594 Msa0127550:CDS
TATGAGTTTGTTGTGCATGG+AGG 0.703519 1_3:-56955638 None:intergenic
GTTAAATGCTGATACCTGTG+AGG 0.736157 1_3:+56956706 Msa0127550:CDS
ATGACGGAGAAGGTGCAATG+CGG 0.752637 1_3:+56956396 Msa0127550:CDS
GTTGATGACTGGATCCATGG+AGG 0.768053 1_3:+56959392 Msa0127550:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATAAATGTATACAGA+AGG - chr1_3:56955860-56955879 None:intergenic 15.0%
!!! GTAAAACATTTTATACATAT+AGG - chr1_3:56957010-56957029 None:intergenic 15.0%
!! ATATGACAATTATGATTTAT+AGG + chr1_3:56957105-56957124 Msa0127550:intron 15.0%
!!! GATTTAAATACGTTATTTTT+TGG + chr1_3:56957162-56957181 Msa0127550:intron 15.0%
!! AGTTTATGTTAATGAATTAC+CGG - chr1_3:56955331-56955350 None:intergenic 20.0%
!! TGTTTAGAAATAAGAATTAG+AGG - chr1_3:56955538-56955557 None:intergenic 20.0%
!!! AACTCATAAAAATTGATTCT+GGG + chr1_3:56955598-56955617 Msa0127550:CDS 20.0%
!!! TGAAGTTAATTCTTTTAGAA+GGG + chr1_3:56956145-56956164 Msa0127550:CDS 20.0%
!!! TTTTTTACTTGACTTACAAT+GGG - chr1_3:56957392-56957411 None:intergenic 20.0%
!!! TAATTACAAGTTTGAAATCA+CGG - chr1_3:56957548-56957567 None:intergenic 20.0%
!! TGTAAATTAAGTTCCTTATT+TGG + chr1_3:56957572-56957591 Msa0127550:intron 20.0%
!!! TTGTTACATATTTTCTCTAT+TGG + chr1_3:56957638-56957657 Msa0127550:intron 20.0%
!!! ACTTTGTTTCAAAACAATTT+TGG + chr1_3:56958475-56958494 Msa0127550:CDS 20.0%
!!! CTTTGTTTCAAAACAATTTT+GGG + chr1_3:56958476-56958495 Msa0127550:CDS 20.0%
!!! TTTGTTTCAAAACAATTTTG+GGG + chr1_3:56958477-56958496 Msa0127550:CDS 20.0%
!! ATAAATATAGAAGAACTACT+TGG + chr1_3:56959418-56959437 Msa0127550:CDS 20.0%
!! GTATGATATATTTAAGATGC+CGG + chr1_3:56955309-56955328 Msa0127550:intron 25.0%
! AATGTAATTGACAATCTTGT+AGG + chr1_3:56955439-56955458 Msa0127550:intron 25.0%
!! GAACTCATAAAAATTGATTC+TGG + chr1_3:56955597-56955616 Msa0127550:CDS 25.0%
!! GTAAACTTTTATGATGATCA+AGG + chr1_3:56955996-56956015 Msa0127550:CDS 25.0%
!! CTGAAGTTAATTCTTTTAGA+AGG + chr1_3:56956144-56956163 Msa0127550:CDS 25.0%
! TTCATAGGACTACTAATATT+AGG - chr1_3:56956332-56956351 None:intergenic 25.0%
! TTGAAGTTCAAAACATTCAT+AGG - chr1_3:56956347-56956366 None:intergenic 25.0%
! TTGAACTTCAAATCAGAAAA+CGG + chr1_3:56956356-56956375 Msa0127550:CDS 25.0%
! AGATTGTAATGATGAAAAGT+TGG + chr1_3:56956490-56956509 Msa0127550:CDS 25.0%
!! ATATGGATTTGATAGCTTTA+TGG + chr1_3:56956594-56956613 Msa0127550:CDS 25.0%
!!! TATGGATTTGATAGCTTTAT+GGG + chr1_3:56956595-56956614 Msa0127550:CDS 25.0%
!! TATTTGACTTCAACCATAAA+TGG - chr1_3:56957076-56957095 None:intergenic 25.0%
! TGACAATTATGATTTATAGG+CGG + chr1_3:56957108-56957127 Msa0127550:intron 25.0%
!!! TGGTTTTATCGTCTATTTTA+AGG + chr1_3:56957182-56957201 Msa0127550:intron 25.0%
! AACTATTAAATACCATAGCA+GGG + chr1_3:56957224-56957243 Msa0127550:intron 25.0%
!!! GTTTTTTACTTGACTTACAA+TGG - chr1_3:56957393-56957412 None:intergenic 25.0%
!! AATTAAGTTCCTTATTTGGT+TGG + chr1_3:56957576-56957595 Msa0127550:intron 25.0%
! AAAACATATCCAACCAAATA+AGG - chr1_3:56957588-56957607 None:intergenic 25.0%
! GTTGTCTAATTTATATCCAA+TGG + chr1_3:56957660-56957679 Msa0127550:intron 25.0%
! TCTAATTTATATCCAATGGT+AGG + chr1_3:56957664-56957683 Msa0127550:intron 25.0%
!! CTATCATTTTCTTTGGATAT+AGG - chr1_3:56957803-56957822 None:intergenic 25.0%
!! CAAAGAAAATGATAGTGTTT+GGG + chr1_3:56957808-56957827 Msa0127550:CDS 25.0%
!! AGAACTTCGTGAATAAATTT+TGG - chr1_3:56958532-56958551 None:intergenic 25.0%
! AAAATTTATTCACGAAGTTC+TGG + chr1_3:56958531-56958550 Msa0127550:CDS 25.0%
! AATGAACTCAAATATTGATG+CGG + chr1_3:56958657-56958676 Msa0127550:CDS 25.0%
!!! AACAAATTTGCTTTGAGAAA+AGG + chr1_3:56958982-56959001 Msa0127550:CDS 25.0%
!! TATTTGAGAATCATTTTCAG+TGG + chr1_3:56959109-56959128 Msa0127550:CDS 25.0%
!! AATCATTTTCAGTGGAAAAA+TGG + chr1_3:56959117-56959136 Msa0127550:CDS 25.0%
ATACGATAATAGCGCTATAA+TGG + chr1_3:56955226-56955245 Msa0127550:CDS 30.0%
! TCAATTTTTATGAGTTCCAC+AGG - chr1_3:56955594-56955613 None:intergenic 30.0%
! TAGCTTTTCTTGATCTTATC+CGG - chr1_3:56955683-56955702 None:intergenic 30.0%
GTAAGAAGATTGAACTTACT+GGG - chr1_3:56955797-56955816 None:intergenic 30.0%
AGTAAGAAGATTGAACTTAC+TGG - chr1_3:56955798-56955817 None:intergenic 30.0%
!!! TTTGTGAGGTTTTTTGTGTA+TGG - chr1_3:56955949-56955968 None:intergenic 30.0%
! GAACATTGGTTATATTTGTG+AGG - chr1_3:56955963-56955982 None:intergenic 30.0%
CACTGAAATAAAAACTCTGA+AGG + chr1_3:56956028-56956047 Msa0127550:CDS 30.0%
! AATAAAAACTCTGAAGGAAG+AGG + chr1_3:56956034-56956053 Msa0127550:CDS 30.0%
! AGTTATTTCGTTGAAGTCTT+TGG - chr1_3:56956094-56956113 None:intergenic 30.0%
!! TACGTAATGTTCAAGTTGTA+TGG - chr1_3:56956471-56956490 None:intergenic 30.0%
TGTAATGATGAAAAGTTGGA+AGG + chr1_3:56956494-56956513 Msa0127550:CDS 30.0%
! TTTCTTTTGAACACCTTCTT+TGG - chr1_3:56956633-56956652 None:intergenic 30.0%
! GATGCTTACATGGATAATTT+TGG + chr1_3:56956737-56956756 Msa0127550:CDS 30.0%
AAAGAAAACGTATCGAAAAG+CGG + chr1_3:56956812-56956831 Msa0127550:CDS 30.0%
ACATCAAGTTATCTTCTTGA+TGG + chr1_3:56956896-56956915 Msa0127550:CDS 30.0%
TTATCTTCTTGATGGAAATG+AGG + chr1_3:56956904-56956923 Msa0127550:CDS 30.0%
TGAAAAGTAAAAGACGAGAT+GGG - chr1_3:56956951-56956970 None:intergenic 30.0%
ATGAAAAGTAAAAGACGAGA+TGG - chr1_3:56956952-56956971 None:intergenic 30.0%
ATTGACTGTTGAACCATTTA+TGG + chr1_3:56957060-56957079 Msa0127550:intron 30.0%
!!! ATCGTCTATTTTAAGGCTAA+AGG + chr1_3:56957189-56957208 Msa0127550:intron 30.0%
CAACTATTAAATACCATAGC+AGG + chr1_3:56957223-56957242 Msa0127550:intron 30.0%
TGACACAAATAAAGAATCCA+AGG + chr1_3:56957271-56957290 Msa0127550:CDS 30.0%
ATGTGAACATGATTATCGAT+TGG + chr1_3:56957298-56957317 Msa0127550:CDS 30.0%
! TATCGATTGGATGAACAAAT+TGG + chr1_3:56957311-56957330 Msa0127550:CDS 30.0%
!!! GTTTTAATTAGCAACGAGTT+TGG + chr1_3:56957432-56957451 Msa0127550:intron 30.0%
TAACTTTCAGAAAATCACAG+TGG - chr1_3:56957473-56957492 None:intergenic 30.0%
! CCAAACACTATCATTTTCTT+TGG - chr1_3:56957810-56957829 None:intergenic 30.0%
! CCAAAGAAAATGATAGTGTT+TGG + chr1_3:56957807-56957826 Msa0127550:CDS 30.0%
!! AAAAAGCTTTCGAGTTTCTT+TGG + chr1_3:56957873-56957892 Msa0127550:CDS 30.0%
! TTTCTTTGGAAAAACATTGC+AGG + chr1_3:56957887-56957906 Msa0127550:CDS 30.0%
! TTCTTTGGAAAAACATTGCA+GGG + chr1_3:56957888-56957907 Msa0127550:CDS 30.0%
AAGAAAAGAGATGATGAGAA+AGG - chr1_3:56957996-56958015 None:intergenic 30.0%
CTTATCTTCTGTTCTCATTA+AGG - chr1_3:56958302-56958321 None:intergenic 30.0%
! GCATTGAGAATTTTACTTTC+TGG + chr1_3:56958451-56958470 Msa0127550:CDS 30.0%
!! TTCGTGAATAAATTTTGGTC+TGG - chr1_3:56958527-56958546 None:intergenic 30.0%
TTAGATTCTAAGTACAGAAG+AGG + chr1_3:56958559-56958578 Msa0127550:CDS 30.0%
TATTGATGCGGAAAAAATGA+AGG + chr1_3:56958669-56958688 Msa0127550:CDS 30.0%
ATTGATGCGGAAAAAATGAA+GGG + chr1_3:56958670-56958689 Msa0127550:CDS 30.0%
AATGTATAGATTTGCAAACC+AGG - chr1_3:56958769-56958788 None:intergenic 30.0%
!!! TAGTTTTTTCAACTCTTCAC+CGG - chr1_3:56958956-56958975 None:intergenic 30.0%
GCTCAAAACAAATCTTACTT+TGG - chr1_3:56959010-56959029 None:intergenic 30.0%
AACTCTATTAAAAACCTCAC+TGG - chr1_3:56959090-56959109 None:intergenic 30.0%
GATAAATTTGAAGATCGTTG+TGG + chr1_3:56959198-56959217 Msa0127550:CDS 30.0%
!!! ATAGCTTGCTTTGTTTTTGA+AGG - chr1_3:56959321-56959340 None:intergenic 30.0%
TCTATATTTATCTTCCTCCA+TGG - chr1_3:56959409-56959428 None:intergenic 30.0%
! TTCTTGTATGATTTTCAGTG+AGG + chr1_3:56959452-56959471 Msa0127550:CDS 30.0%
AGATTGAAGATGAAATACTC+AGG + chr1_3:56959511-56959530 Msa0127550:CDS 30.0%
GATTGAAGATGAAATACTCA+GGG + chr1_3:56959512-56959531 Msa0127550:CDS 30.0%
!! AGCGCTATAATGGATATTCA+TGG + chr1_3:56955236-56955255 Msa0127550:CDS 35.0%
TAACTAGATGTGCTATGCAT+TGG + chr1_3:56955389-56955408 Msa0127550:intron 35.0%
! GCATTTGAAGCAGTATTGAA+TGG + chr1_3:56955558-56955577 Msa0127550:intron 35.0%
!! TACTATGAGTTTGTTGTGCA+TGG - chr1_3:56955644-56955663 None:intergenic 35.0%
! GTAATTTGTCATCAGACATC+CGG + chr1_3:56955661-56955680 Msa0127550:CDS 35.0%
ATATGTCAATTCCAGGTCTT+AGG - chr1_3:56955728-56955747 None:intergenic 35.0%
CATGAAATCGACATGAACAT+TGG - chr1_3:56955977-56955996 None:intergenic 35.0%
TATGATGATCAAGGTTCACT+TGG + chr1_3:56956005-56956024 Msa0127550:CDS 35.0%
AAGGAAGAGGATAATGTGAT+TGG + chr1_3:56956047-56956066 Msa0127550:CDS 35.0%
TATTTCGTTGAAGTCTTTGG+AGG - chr1_3:56956091-56956110 None:intergenic 35.0%
CTTCAACGAAATAACTGTGA+AGG + chr1_3:56956098-56956117 Msa0127550:CDS 35.0%
!!! CCACAATCTAGGTTACTTTT+CGG + chr1_3:56956194-56956213 Msa0127550:CDS 35.0%
!!! CACAATCTAGGTTACTTTTC+GGG + chr1_3:56956195-56956214 Msa0127550:CDS 35.0%
! AGTTTTTGTCTGATCTTTCC+TGG + chr1_3:56956219-56956238 Msa0127550:CDS 35.0%
! AACCTTCTTTAAAGATGACG+CGG - chr1_3:56956252-56956271 None:intergenic 35.0%
GGTTGCATTTGTCATAAGAT+CGG + chr1_3:56956268-56956287 Msa0127550:CDS 35.0%
AAGCTTGTGCATCAAGTTAT+GGG + chr1_3:56956299-56956318 Msa0127550:CDS 35.0%
TCACTTGAGCATGATTATGA+CGG + chr1_3:56956380-56956399 Msa0127550:CDS 35.0%
AATGATGAAAAGTTGGAAGG+TGG + chr1_3:56956497-56956516 Msa0127550:CDS 35.0%
! AGGTGGTGATAATGTTTCTT+CGG + chr1_3:56956514-56956533 Msa0127550:CDS 35.0%
TAAGAGAAGGTGGTTGTTTA+AGG + chr1_3:56956568-56956587 Msa0127550:CDS 35.0%
TATCGATGATCATGAAGAAC+AGG + chr1_3:56956664-56956683 Msa0127550:CDS 35.0%
TGTCTTTCTTCACTGATAGT+TGG - chr1_3:56956779-56956798 None:intergenic 35.0%
GGTAGAAAATCCTGATGATT+TGG + chr1_3:56956841-56956860 Msa0127550:CDS 35.0%
ATCATGTTCACATGTTTCCT+TGG - chr1_3:56957291-56957310 None:intergenic 35.0%
! TTACAAGTTTGAAATCACGG+TGG - chr1_3:56957545-56957564 None:intergenic 35.0%
GAAACAATGCAGTGAAGATA+CGG + chr1_3:56957715-56957734 Msa0127550:CDS 35.0%
!! TGCATGTTTTCTTTGAGTTC+AGG - chr1_3:56957842-56957861 None:intergenic 35.0%
!!! GAAACTCGAAAGCTTTTTTC+TGG - chr1_3:56957871-56957890 None:intergenic 35.0%
AAAGAGAAATCTGACACAAG+TGG + chr1_3:56957932-56957951 Msa0127550:CDS 35.0%
GGCAAAAAGAGTTCGAAAAA+TGG + chr1_3:56958086-56958105 Msa0127550:CDS 35.0%
TTCAACGACACCAAAATCAA+TGG + chr1_3:56958159-56958178 Msa0127550:CDS 35.0%
TGCTTGACACCATTAGAATT+TGG - chr1_3:56958199-56958218 None:intergenic 35.0%
! TAATGGTGTCAAGCATGATT+TGG + chr1_3:56958204-56958223 Msa0127550:CDS 35.0%
GTCTAGAAAAGATCAAAAGC+TGG + chr1_3:56958230-56958249 Msa0127550:CDS 35.0%
AACTTTGATGTGGTACTTCT+AGG - chr1_3:56958403-56958422 None:intergenic 35.0%
!! AAAGTTGAGGAAGTGCTTAA+TGG + chr1_3:56958417-56958436 Msa0127550:CDS 35.0%
AGTAAAATTCTCAATGCTGC+TGG - chr1_3:56958448-56958467 None:intergenic 35.0%
GTGCTTCGAAAAATCACAAA+TGG + chr1_3:56958700-56958719 Msa0127550:CDS 35.0%
GCAGAAACTGCAAAAGAAAA+TGG + chr1_3:56958825-56958844 Msa0127550:CDS 35.0%
GAAACTGCAAAAGAAAATGG+CGG + chr1_3:56958828-56958847 Msa0127550:CDS 35.0%
AAACTGCAAAAGAAAATGGC+GGG + chr1_3:56958829-56958848 Msa0127550:CDS 35.0%
!! GTTGAGCTTTTGATAACACT+CGG + chr1_3:56958874-56958893 Msa0127550:CDS 35.0%
!! TTGAGCTTTTGATAACACTC+GGG + chr1_3:56958875-56958894 Msa0127550:CDS 35.0%
AAAGAGATTCTGCAACTAAC+TGG + chr1_3:56959141-56959160 Msa0127550:CDS 35.0%
TCAATCAATGCTTGTTCTGA+AGG + chr1_3:56959243-56959262 Msa0127550:CDS 35.0%
! AGCTTCAAGAGTGATTTTCT+TGG + chr1_3:56959281-56959300 Msa0127550:CDS 35.0%
! TGATTTTCTTGGATTCTGAG+TGG + chr1_3:56959292-56959311 Msa0127550:CDS 35.0%
! TAAACCATTTTCTCTTGACC+AGG - chr1_3:56959360-56959379 None:intergenic 35.0%
!! GTATATCACTTGTTGATGAC+TGG + chr1_3:56959381-56959400 Msa0127550:CDS 35.0%
AAGAACTACTTGGAAAGAGT+GGG + chr1_3:56959428-56959447 Msa0127550:CDS 35.0%
GATACATGTGTCTAACTCGT+TGG + chr1_3:56955271-56955290 Msa0127550:intron 40.0%
! AAGCAGTATTGAATGGCTCA+TGG + chr1_3:56955565-56955584 Msa0127550:CDS 40.0%
! AGCAGTATTGAATGGCTCAT+GGG + chr1_3:56955566-56955585 Msa0127550:CDS 40.0%
TTGTGCATGGAGGAAATGTA+TGG - chr1_3:56955631-56955650 None:intergenic 40.0%
!! TATGAGTTTGTTGTGCATGG+AGG - chr1_3:56955641-56955660 None:intergenic 40.0%
AATTCCAGGTCTTAGGAAAC+TGG - chr1_3:56955721-56955740 None:intergenic 40.0%
AGCACAGATATGTCAATTCC+AGG - chr1_3:56955735-56955754 None:intergenic 40.0%
CCGAAAAGTAACCTAGATTG+TGG - chr1_3:56956197-56956216 None:intergenic 40.0%
! TACCGCGTCATCTTTAAAGA+AGG + chr1_3:56956247-56956266 Msa0127550:CDS 40.0%
CAAGCTTGTGCATCAAGTTA+TGG + chr1_3:56956298-56956317 Msa0127550:CDS 40.0%
! GGTGGTGATAATGTTTCTTC+GGG + chr1_3:56956515-56956534 Msa0127550:CDS 40.0%
GCACAAAGAAGCTTAAGAGA+AGG + chr1_3:56956555-56956574 Msa0127550:CDS 40.0%
CAAAGAAGCTTAAGAGAAGG+TGG + chr1_3:56956558-56956577 Msa0127550:CDS 40.0%
! GTGGTTGTTTAAGGAGCATA+TGG + chr1_3:56956577-56956596 Msa0127550:CDS 40.0%
GAAGCGATACAATCCAAAGA+AGG + chr1_3:56956617-56956636 Msa0127550:CDS 40.0%
CATGAAGAACAGGATCATAG+AGG + chr1_3:56956674-56956693 Msa0127550:CDS 40.0%
GTTAAATGCTGATACCTGTG+AGG + chr1_3:56956706-56956725 Msa0127550:CDS 40.0%
GGAAGTAATCGATGCTTACA+TGG + chr1_3:56956727-56956746 Msa0127550:CDS 40.0%
TGATAGTTGGATCTTCTGGT+AGG - chr1_3:56956766-56956785 None:intergenic 40.0%
TCACTGATAGTTGGATCTTC+TGG - chr1_3:56956770-56956789 None:intergenic 40.0%
TGAAGAAAGACAGTTTGAGC+AGG + chr1_3:56956787-56956806 Msa0127550:CDS 40.0%
! GAAAATCCTGATGATTTGGC+TGG + chr1_3:56956845-56956864 Msa0127550:CDS 40.0%
! TGATGATTTGGCTGGCATAT+GGG + chr1_3:56956853-56956872 Msa0127550:CDS 40.0%
! TTTTCACAAAGCCGCATGTA+CGG - chr1_3:56957346-56957365 None:intergenic 40.0%
ATATCCAATGGTAGGTGACA+AGG + chr1_3:56957672-56957691 Msa0127550:intron 40.0%
TCAACTGATGCTACTGATCT+TGG + chr1_3:56957773-56957792 Msa0127550:CDS 40.0%
GAAAAACATTGCAGGGTCTA+TGG + chr1_3:56957895-56957914 Msa0127550:CDS 40.0%
TGTGTTATATCTCATGCTCC+TGG + chr1_3:56957959-56957978 Msa0127550:CDS 40.0%
! AGGACTAATGGTCTTTTCTG+TGG - chr1_3:56958040-56958059 None:intergenic 40.0%
! TTTTTGCCATGTGTAGAGTG+TGG - chr1_3:56958074-56958093 None:intergenic 40.0%
AAAATGGAATATTCCCGTGC+CGG + chr1_3:56958102-56958121 Msa0127550:CDS 40.0%
!! TTGGTGTCGTTGAATGTATC+TGG - chr1_3:56958153-56958172 None:intergenic 40.0%
! CCATTAGAATTTGGAACTCC+AGG - chr1_3:56958190-56958209 None:intergenic 40.0%
CCTGGAGTTCCAAATTCTAA+TGG + chr1_3:56958187-56958206 Msa0127550:CDS 40.0%
AACCCATGACAAGAACACTA+GGG - chr1_3:56958264-56958283 None:intergenic 40.0%
TAACCCATGACAAGAACACT+AGG - chr1_3:56958265-56958284 None:intergenic 40.0%
! AAATTCACACAGAAAGCGCA+TGG + chr1_3:56958324-56958343 Msa0127550:CDS 40.0%
TCATCAAGTATCAAGAGTCC+AGG - chr1_3:56958370-56958389 None:intergenic 40.0%
TGGACTCTTGATACTTGATG+AGG + chr1_3:56958369-56958388 Msa0127550:CDS 40.0%
GGACTCTTGATACTTGATGA+GGG + chr1_3:56958370-56958389 Msa0127550:CDS 40.0%
! GAAGTACCACATCAAAGTTG+AGG + chr1_3:56958404-56958423 Msa0127550:CDS 40.0%
AGTTCTTCTGATACTCTTCC+TGG + chr1_3:56958748-56958767 Msa0127550:CDS 40.0%
! CACAATCTCGTGTTGTTCAT+CGG - chr1_3:56958806-56958825 None:intergenic 40.0%
!!! CTGCTGTTTTGATCAACCAT+GGG - chr1_3:56958909-56958928 None:intergenic 40.0%
!!! TCTGCTGTTTTGATCAACCA+TGG - chr1_3:56958910-56958929 None:intergenic 40.0%
! TGTGCAGCTAAGTTTTTCTC+CGG + chr1_3:56958934-56958953 Msa0127550:CDS 40.0%
TTAAAAACCTCACTGGTGCA+AGG - chr1_3:56959083-56959102 None:intergenic 40.0%
GAAGAACTACTTGGAAAGAG+TGG + chr1_3:56959427-56959446 Msa0127550:CDS 40.0%
TTGTTGAAGATCCTTCCGAA+TGG + chr1_3:56959478-56959497 Msa0127550:CDS 40.0%
AATCTTCTCAGCTTGCCATT+CGG - chr1_3:56959496-56959515 None:intergenic 40.0%
AGATGAAATACTCAGGGAGA+TGG + chr1_3:56959518-56959537 Msa0127550:CDS 40.0%
GCTCTTCATTGCCACAATCT+AGG + chr1_3:56956183-56956202 Msa0127550:CDS 45.0%
! GAGCATGATTATGACGGAGA+AGG + chr1_3:56956386-56956405 Msa0127550:CDS 45.0%
CCAATTTCGCTTCTTGGCAT+TGG - chr1_3:56956422-56956441 None:intergenic 45.0%
CCAATGCCAAGAAGCGAAAT+TGG + chr1_3:56956419-56956438 Msa0127550:CDS 45.0%
ATGCCAAGAAGCGAAATTGG+AGG + chr1_3:56956422-56956441 Msa0127550:CDS 45.0%
AGTCCTCCAATTTCGCTTCT+TGG - chr1_3:56956428-56956447 None:intergenic 45.0%
!! AGCATCGATTACTTCCTCAC+AGG - chr1_3:56956723-56956742 None:intergenic 45.0%
CATATGCCAGCCAAATCATC+AGG - chr1_3:56956854-56956873 None:intergenic 45.0%
! CTGATGATTTGGCTGGCATA+TGG + chr1_3:56956852-56956871 Msa0127550:CDS 45.0%
GGAATATACTGCCGTACATG+CGG + chr1_3:56957332-56957351 Msa0127550:CDS 45.0%
! GTTTGAAATCACGGTGGATC+TGG - chr1_3:56957539-56957558 None:intergenic 45.0%
!! TTAGCCTTGTCACCTACCAT+TGG - chr1_3:56957679-56957698 None:intergenic 45.0%
GGTAGGTGACAAGGCTAAAA+TGG + chr1_3:56957681-56957700 Msa0127550:intron 45.0%
GAGAAATCTGACACAAGTGG+TGG + chr1_3:56957935-56957954 Msa0127550:CDS 45.0%
! AGAAAGGTTTTTCCAGCTCC+AGG - chr1_3:56957980-56957999 None:intergenic 45.0%
!! GTCTTTGGAGCTAGGACTAA+TGG - chr1_3:56958052-56958071 None:intergenic 45.0%
CATGTGTAGAGTGTGGTCTT+TGG - chr1_3:56958067-56958086 None:intergenic 45.0%
CAAAGACCACACTCTACACA+TGG + chr1_3:56958065-56958084 Msa0127550:CDS 45.0%
CAGCTGTGGATAAGATACAC+CGG - chr1_3:56958124-56958143 None:intergenic 45.0%
!!! CCAGGCAGAACCATTGATTT+TGG - chr1_3:56958172-56958191 None:intergenic 45.0%
! CCAAAATCAATGGTTCTGCC+TGG + chr1_3:56958169-56958188 Msa0127550:CDS 45.0%
ACACCCTAGTGTTCTTGTCA+TGG + chr1_3:56958258-56958277 Msa0127550:CDS 45.0%
CACCCTAGTGTTCTTGTCAT+GGG + chr1_3:56958259-56958278 Msa0127550:CDS 45.0%
!! AAAGCATTGAGGGAAAGTCC+TGG + chr1_3:56958349-56958368 Msa0127550:CDS 45.0%
GCACTTCCTCAACTTTGATG+TGG - chr1_3:56958413-56958432 None:intergenic 45.0%
GTACAGAAGAGGCAAACTAG+AGG + chr1_3:56958570-56958589 Msa0127550:CDS 45.0%
ACGCGCGAGAAAATTCTTCT+TGG + chr1_3:56958618-56958637 Msa0127550:CDS 45.0%
CTCAACCTCAAGAGAGTAAC+CGG - chr1_3:56958860-56958879 None:intergenic 45.0%
TGCTCATCACACGTGAGATT+AGG - chr1_3:56959032-56959051 None:intergenic 45.0%
AATCTCACGTGTGATGAGCA+AGG + chr1_3:56959032-56959051 Msa0127550:CDS 45.0%
ACGTGTGATGAGCAAGGAAA+AGG + chr1_3:56959038-56959057 Msa0127550:CDS 45.0%
TCGACCTGGTCAAGAGAAAA+TGG + chr1_3:56959353-56959372 Msa0127550:CDS 45.0%
!! CTTGTTGATGACTGGATCCA+TGG + chr1_3:56959389-56959408 Msa0127550:CDS 45.0%
TGCTCCAGTTTCCTAAGACC+TGG + chr1_3:56955714-56955733 Msa0127550:CDS 50.0%
AGATGACGCGGTAACAAACC+AGG - chr1_3:56956240-56956259 None:intergenic 50.0%
! ATGACGGAGAAGGTGCAATG+CGG + chr1_3:56956396-56956415 Msa0127550:CDS 50.0%
GACTACGACGCTCAAAACGT+AGG + chr1_3:56956444-56956463 Msa0127550:CDS 50.0%
CGTATCGAAAAGCGGTGATG+AGG + chr1_3:56956820-56956839 Msa0127550:CDS 50.0%
TCAGCATTTGCACCCTGCTA+TGG - chr1_3:56957239-56957258 None:intergenic 50.0%
ATATCTCATGCTCCTGGAGC+TGG + chr1_3:56957965-56957984 Msa0127550:CDS 50.0%
!! AGAGTGTGGTCTTTGGAGCT+AGG - chr1_3:56958060-56958079 None:intergenic 50.0%
TGGATAAGATACACCGGCAC+GGG - chr1_3:56958118-56958137 None:intergenic 50.0%
GTGGATAAGATACACCGGCA+CGG - chr1_3:56958119-56958138 None:intergenic 50.0%
!! AGCGCATGGCTAAAGCATTG+AGG + chr1_3:56958338-56958357 Msa0127550:CDS 50.0%
!! GCGCATGGCTAAAGCATTGA+GGG + chr1_3:56958339-56958358 Msa0127550:CDS 50.0%
CAGAAGAGGCAAACTAGAGG+AGG + chr1_3:56958573-56958592 Msa0127550:CDS 50.0%
!! GGAAGCGCCTCATTTACTAG+AGG + chr1_3:56958594-56958613 Msa0127550:CDS 50.0%
!! AAGAAAATGGCGGGCTGTAC+CGG + chr1_3:56958838-56958857 Msa0127550:CDS 50.0%
!! CTGTACCGGTTACTCTCTTG+AGG + chr1_3:56958852-56958871 Msa0127550:CDS 50.0%
!! GTTGATGACTGGATCCATGG+AGG + chr1_3:56959392-56959411 Msa0127550:CDS 50.0%
TTCTCAGCTTGCCATTCGGA+AGG - chr1_3:56959492-56959511 None:intergenic 50.0%
ACTCAGGGAGATGGTTGAAG+AGG + chr1_3:56959527-56959546 Msa0127550:CDS 50.0%
GAATGGCTCATGGGAACCTG+TGG + chr1_3:56955575-56955594 Msa0127550:CDS 55.0%
GCAGGGTGCAAATGCTGATG+AGG + chr1_3:56957241-56957260 Msa0127550:intron 55.0%
! GTATCTGGCTTCGACAGCTG+TGG - chr1_3:56958138-56958157 None:intergenic 55.0%
GCGCGTGCCTCTAGTAAATG+AGG - chr1_3:56958604-56958623 None:intergenic 55.0%
CACTCGGGTCAATACACCCA+TGG + chr1_3:56958890-56958909 Msa0127550:CDS 55.0%
GTCACAACCTTGCACCAGTG+AGG + chr1_3:56959073-56959092 Msa0127550:CDS 55.0%
! ATTGCACGCGCTTTTCGACC+TGG + chr1_3:56959339-56959358 Msa0127550:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 56955185 56959605 56955185 ID=Msa0127550;Name=Msa0127550
chr1_3 mRNA 56955185 56959605 56955185 ID=Msa0127550-mRNA-1;Parent=Msa0127550;Name=Msa0127550-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0|0|0.8|1|1|5|0|1131
chr1_3 exon 56955185 56955266 56955185 ID=Msa0127550-mRNA-1:exon:18008;Parent=Msa0127550-mRNA-1
chr1_3 exon 56955559 56955797 56955559 ID=Msa0127550-mRNA-1:exon:18009;Parent=Msa0127550-mRNA-1
chr1_3 exon 56955921 56956925 56955921 ID=Msa0127550-mRNA-1:exon:18010;Parent=Msa0127550-mRNA-1
chr1_3 exon 56957245 56957394 56957245 ID=Msa0127550-mRNA-1:exon:18011;Parent=Msa0127550-mRNA-1
chr1_3 exon 56957686 56959605 56957686 ID=Msa0127550-mRNA-1:exon:18012;Parent=Msa0127550-mRNA-1
chr1_3 CDS 56955185 56955266 56955185 ID=Msa0127550-mRNA-1:cds;Parent=Msa0127550-mRNA-1
chr1_3 CDS 56955559 56955797 56955559 ID=Msa0127550-mRNA-1:cds;Parent=Msa0127550-mRNA-1
chr1_3 CDS 56955921 56956925 56955921 ID=Msa0127550-mRNA-1:cds;Parent=Msa0127550-mRNA-1
chr1_3 CDS 56957245 56957394 56957245 ID=Msa0127550-mRNA-1:cds;Parent=Msa0127550-mRNA-1
chr1_3 CDS 56957686 56959605 56957686 ID=Msa0127550-mRNA-1:cds;Parent=Msa0127550-mRNA-1
Gene Sequence

>Msa0127550

ATGGATTACAACATTGACTTTGTGATATTGAAAAGATTGTCATACGATAATAGCGCTATAATGGATATTCATGGTGCACATACATTTGAAGCAGTATTGAATGGCTCATGGGAACCTGTGGAACTCATAAAAATTGATTCTGGGAAAACTACCATACATTTCCTCCATGCACAACAAACTCATAGTAATTTGTCATCAGACATCCGGATAAGATCAAGAAAAGCTACTTCGACTGATTGCTCCAGTTTCCTAAGACCTGGAATTGACATATCTGTGCTATCAAGCTTTCGATACGATAAAAACTCACGTCAATCTAGCCCAATGTCGACTGATGCTAGAGTGAATTCCATACACAAAAAACCTCACAAATATAACCAATGTTCATGTCGATTTCATGTAAACTTTTATGATGATCAAGGTTCACTTGGCACTGAAATAAAAACTCTGAAGGAAGAGGATAATGTGATTGGAATTGAGAAAATTTTCATCCTCCAAAGACTTCAACGAAATAACTGTGAAGGATCGTTAAGAGAAAATGATTGTTCTGAAGTTAATTCTTTTAGAAGGGATTCATCAGAAGACTGCTCTTCATTGCCACAATCTAGGTTACTTTTCGGGAAGTTTTTGTCTGATCTTTCCTGGTTTGTTACCGCGTCATCTTTAAAGAAGGTTGCATTTGTCATAAGATCGGTTCAAAACAAGCTTGTGCATCAAGTTATGGGAAATGATCCTAATATTAGTAGTCCTATGAATGTTTTGAACTTCAAATCAGAAAACGGATCACTTGAGCATGATTATGACGGAGAAGGTGCAATGCGGCCAATGCCAAGAAGCGAAATTGGAGGACTACGACGCTCAAAACGTAGGACCATACAACTTGAACATTACGTAGATTGTAATGATGAAAAGTTGGAAGGTGGTGATAATGTTTCTTCGGGACATGTTAATAATTTCTGCACAAAGAAGCTTAAGAGAAGGTGGTTGTTTAAGGAGCATATGGATTTGATAGCTTTATGGGAAGCGATACAATCCAAAGAAGGTGTTCAAAAGAAAACGACAAACAGTATCGATGATCATGAAGAACAGGATCATAGAGGCAGTAGAACGTTAAATGCTGATACCTGTGAGGAAGTAATCGATGCTTACATGGATAATTTTGGTAGCCTACCAGAAGATCCAACTATCAGTGAAGAAAGACAGTTTGAGCAGGAGAAAGAAAACGTATCGAAAAGCGGTGATGAGGTAGAAAATCCTGATGATTTGGCTGGCATATGGGAAGAAATGAATACAGCTTTGACATCAAGTTATCTTCTTGATGGAAATGAGGGTGCAAATGCTGATGAGGTTTTAGCTGACACAAATAAAGAATCCAAGGAAACATGTGAACATGATTATCGATTGGATGAACAAATTGGAATATACTGCCGTACATGCGGCTTTGTGAAAACTGAGATACGATACATTTCTGAACCCATTGTGACAAGGCTAAAATGGTACGAACAGAAGAAACAATGCAGTGAAGATACGGAACAAGTTGATGAAGATGATAATAATGATACGTTCTCAACTGATGCTACTGATCTTGGTGAACCTATATCCAAAGAAAATGATAGTGTTTGGGAATTGATTCCTGAACTCAAAGAAAACATGCATGCACACCAGAAAAAAGCTTTCGAGTTTCTTTGGAAAAACATTGCAGGGTCTATGGATCAATCACTCATGAAAGAGAAATCTGACACAAGTGGTGGCTGTGTTATATCTCATGCTCCTGGAGCTGGAAAAACCTTTCTCATCATCTCTTTTCTTGTAAGCTATTTGAAATTATTTCCACAGAAAAGACCATTAGTCCTAGCTCCAAAGACCACACTCTACACATGGCAAAAAGAGTTCGAAAAATGGAATATTCCCGTGCCGGTGTATCTTATCCACAGCTGTCGAAGCCAGATACATTCAACGACACCAAAATCAATGGTTCTGCCTGGAGTTCCAAATTCTAATGGTGTCAAGCATGATTTGGATTGTCTAGAAAAGATCAAAAGCTGGAATTCACACCCTAGTGTTCTTGTCATGGGTTATTCTTCATTTTTAGCCTTAATGAGAACAGAAGATAAGAAAAATTCACACAGAAAGCGCATGGCTAAAGCATTGAGGGAAAGTCCTGGACTCTTGATACTTGATGAGGGACACAATCCTAGAAGTACCACATCAAAGTTGAGGAAGTGCTTAATGGATCTACCAGCAGCATTGAGAATTTTACTTTCTGGCACTTTGTTTCAAAACAATTTTGGGGAATACTTCAACACTCTCTGTTTAGCCAGACCAAAATTTATTCACGAAGTTCTGGAAGAGTTAGATTCTAAGTACAGAAGAGGCAAACTAGAGGAGGAAGCGCCTCATTTACTAGAGGCACGCGCGAGAAAATTCTTCTTGGAGAACATTGAAAAGAAAATGAACTCAAATATTGATGCGGAAAAAATGAAGGGTGTAGATGTGCTTCGAAAAATCACAAATGGTTTTATAGATGTTTATGACAGTGCAAGTTCTTCTGATACTCTTCCTGGTTTGCAAATCTATACATTGCTCGTGAATGCATCCGATGAACAACACGAGATTGTGCAGAAACTGCAAAAGAAAATGGCGGGCTGTACCGGTTACTCTCTTGAGGTTGAGCTTTTGATAACACTCGGGTCAATACACCCATGGTTGATCAAAACAGCAGAAACATGTGCAGCTAAGTTTTTCTCCGGTGAAGAGTTGAAAAAACTAGACGAAAACAAATTTGCTTTGAGAAAAGGTTCCAAAGTAAGATTTGTTTTGAGCCTAATCTCACGTGTGATGAGCAAGGAAAAGGTTCTTATCTTCTGTCACAACCTTGCACCAGTGAGGTTTTTAATAGAGTTATTTGAGAATCATTTTCAGTGGAAAAATGGTAAAGAGATTCTGCAACTAACTGGTGAACAAGACTTCTTAGAAAGAACGAATGTGATAGATAAATTTGAAGATCGTTGTGGCGATTCAAAGATACTTCTTGCTTCAATCAATGCTTGTTCTGAAGGTATCAGTTTAACATCAGCTTCAAGAGTGATTTTCTTGGATTCTGAGTGGAATCCTTCAAAAACAAAGCAAGCTATTGCACGCGCTTTTCGACCTGGTCAAGAGAAAATGGTTTATGTATATCACTTGTTGATGACTGGATCCATGGAGGAAGATAAATATAGAAGAACTACTTGGAAAGAGTGGGTTTCTTGTATGATTTTCAGTGAGGAGCTTGTTGAAGATCCTTCCGAATGGCAAGCTGAGAAGATTGAAGATGAAATACTCAGGGAGATGGTTGAAGAGGACAAGTCTAAACTCATTCATATGATTATGAAGAATGAAAAGACTTCAACAAGCTAA
Protein sequence

>Msa0127550

MDYNIDFVILKRLSYDNSAIMDIHGAHTFEAVLNGSWEPVELIKIDSGKTTIHFLHAQQTHSNLSSDIRIRSRKATSTDCSSFLRPGIDISVLSSFRYDKNSRQSSPMSTDARVNSIHKKPHKYNQCSCRFHVNFYDDQGSLGTEIKTLKEEDNVIGIEKIFILQRLQRNNCEGSLRENDCSEVNSFRRDSSEDCSSLPQSRLLFGKFLSDLSWFVTASSLKKVAFVIRSVQNKLVHQVMGNDPNISSPMNVLNFKSENGSLEHDYDGEGAMRPMPRSEIGGLRRSKRRTIQLEHYVDCNDEKLEGGDNVSSGHVNNFCTKKLKRRWLFKEHMDLIALWEAIQSKEGVQKKTTNSIDDHEEQDHRGSRTLNADTCEEVIDAYMDNFGSLPEDPTISEERQFEQEKENVSKSGDEVENPDDLAGIWEEMNTALTSSYLLDGNEGANADEVLADTNKESKETCEHDYRLDEQIGIYCRTCGFVKTEIRYISEPIVTRLKWYEQKKQCSEDTEQVDEDDNNDTFSTDATDLGEPISKENDSVWELIPELKENMHAHQKKAFEFLWKNIAGSMDQSLMKEKSDTSGGCVISHAPGAGKTFLIISFLVSYLKLFPQKRPLVLAPKTTLYTWQKEFEKWNIPVPVYLIHSCRSQIHSTTPKSMVLPGVPNSNGVKHDLDCLEKIKSWNSHPSVLVMGYSSFLALMRTEDKKNSHRKRMAKALRESPGLLILDEGHNPRSTTSKLRKCLMDLPAALRILLSGTLFQNNFGEYFNTLCLARPKFIHEVLEELDSKYRRGKLEEEAPHLLEARARKFFLENIEKKMNSNIDAEKMKGVDVLRKITNGFIDVYDSASSSDTLPGLQIYTLLVNASDEQHEIVQKLQKKMAGCTGYSLEVELLITLGSIHPWLIKTAETCAAKFFSGEELKKLDENKFALRKGSKVRFVLSLISRVMSKEKVLIFCHNLAPVRFLIELFENHFQWKNGKEILQLTGEQDFLERTNVIDKFEDRCGDSKILLASINACSEGISLTSASRVIFLDSEWNPSKTKQAIARAFRPGQEKMVYVYHLLMTGSMEEDKYRRTTWKEWVSCMIFSEELVEDPSEWQAEKIEDEILREMVEEDKSKLIHMIMKNEKTSTS*