AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0130450

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0130450 MtrunA17_Chr1g0198651 97.938 291 6 0 1 291 1 291 0.0 600
Msa0130450 MtrunA17_Chr5g0422571 62.633 281 90 5 1 275 1 272 1.59e-113 329
Msa0130450 MtrunA17_Chr1g0173841 65.094 212 60 2 75 286 188 385 2.64e-99 296
Msa0130450 MtrunA17_Chr7g0258011 71.809 188 39 2 99 286 173 346 2.75e-99 295
Msa0130450 MtrunA17_Chr2g0328921 71.429 189 40 3 99 286 135 310 1.68e-96 286
Msa0130450 MtrunA17_Chr8g0352011 69.189 185 43 2 99 283 163 333 4.38e-96 286
Msa0130450 MtrunA17_Chr4g0026521 68.254 189 45 4 99 284 115 291 1.96e-95 283
Msa0130450 MtrunA17_Chr2g0321291 69.312 189 42 4 99 284 130 305 3.30e-95 283
Msa0130450 MtrunA17_Chr4g0053341 68.391 174 50 2 87 260 61 229 1.20e-86 259
Msa0130450 MtrunA17_Chr1g0200421 41.447 152 83 6 107 255 244 392 6.36e-27 109
Msa0130450 MtrunA17_Chr2g0320341 38.415 164 92 8 105 263 235 394 2.24e-23 98.6
Msa0130450 MtrunA17_Chr1g0181381 31.111 225 137 7 60 279 8 219 1.33e-21 94.4
Msa0130450 MtrunA17_Chr7g0263941 35.862 145 78 5 107 249 55 186 4.67e-21 92.4
Msa0130450 MtrunA17_Chr3g0123941 35.000 180 97 8 117 283 139 311 7.57e-21 90.9
Msa0130450 MtrunA17_Chr1g0163001 31.188 202 124 5 50 249 7 195 1.09e-20 92.0
Msa0130450 MtrunA17_Chr1g0210821 35.862 145 78 5 107 249 65 196 1.54e-20 91.7
Msa0130450 MtrunA17_Chr1g0185121 34.667 150 84 5 107 254 61 198 1.78e-20 90.9
Msa0130450 MtrunA17_Chr4g0029521 34.507 142 81 4 109 249 61 191 1.83e-20 91.3
Msa0130450 MtrunA17_Chr8g0382121 37.324 142 83 5 117 255 127 265 2.55e-20 89.0
Msa0130450 MtrunA17_Chr1g0199101 35.172 145 79 5 107 249 68 199 3.52e-20 90.5
Msa0130450 MtrunA17_Chr8g0342421 33.333 156 89 6 109 262 85 227 5.01e-20 89.7
Msa0130450 MtrunA17_Chr1g0162971 37.063 143 75 5 109 249 64 193 5.49e-20 89.4
Msa0130450 MtrunA17_Chr1g0151351 34.161 161 91 6 91 249 47 194 6.18e-20 89.4
Msa0130450 MtrunA17_Chr7g0268961 35.664 143 77 6 109 249 45 174 8.81e-20 89.0
Msa0130450 MtrunA17_Chr2g0326381 35.211 142 80 4 109 249 55 185 1.12e-19 88.2
Msa0130450 MtrunA17_Chr1g0191991 33.520 179 90 8 103 273 74 231 1.32e-19 88.6
Msa0130450 MtrunA17_Chr2g0330171 35.172 145 79 6 107 249 67 198 3.97e-19 87.0
Msa0130450 MtrunA17_Chr8g0373541 32.240 183 102 7 85 260 29 196 9.61e-19 86.3
Msa0130450 MtrunA17_Chr4g0040571 33.553 152 88 5 107 257 60 199 3.55e-18 84.3
Msa0130450 MtrunA17_Chr3g0141431 30.405 148 89 5 107 252 43 178 7.98e-17 80.1
Msa0130450 MtrunA17_Chr2g0324991 35.461 141 76 7 107 244 13 141 1.97e-16 74.7
Msa0130450 MtrunA17_Chr8g0375521 27.083 192 124 6 61 248 2 181 5.19e-13 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0130450 AT1G13290.1 65.854 246 60 5 23 258 12 243 1.66e-108 317
Msa0130450 AT3G57670.1 71.429 196 40 4 99 292 201 382 7.42e-98 293
Msa0130450 AT1G51220.1 75.152 165 38 1 100 264 163 324 8.43e-95 283
Msa0130450 AT3G20880.1 77.987 159 32 1 97 255 238 393 8.64e-94 283
Msa0130450 AT1G08290.1 73.810 168 38 3 99 265 164 326 5.80e-93 279
Msa0130450 AT1G34790.1 75.159 157 36 1 100 256 130 283 4.48e-88 265
Msa0130450 AT1G34370.3 41.176 153 84 5 107 255 27 177 3.51e-26 105
Msa0130450 AT1G34370.2 41.176 153 84 5 107 255 237 387 1.40e-25 106
Msa0130450 AT1G34370.1 41.176 153 84 5 107 255 237 387 1.40e-25 106
Msa0130450 AT5G22890.1 40.645 155 84 7 106 255 209 360 1.86e-24 101
Msa0130450 AT5G03150.1 35.211 142 80 4 109 249 76 206 5.82e-21 92.8
Msa0130450 AT1G55110.3 33.758 157 90 5 107 261 84 228 7.57e-21 92.4
Msa0130450 AT1G55110.1 33.758 157 90 5 107 261 84 228 7.57e-21 92.4
Msa0130450 AT1G55110.2 33.758 157 90 5 107 261 84 228 7.57e-21 92.4
Msa0130450 AT5G44160.1 32.178 202 106 8 63 257 20 197 2.44e-20 90.9
Msa0130450 AT2G02070.2 35.443 158 87 5 94 249 60 204 3.44e-20 90.9
Msa0130450 AT2G02070.1 35.443 158 87 5 94 249 60 204 3.44e-20 90.9
Msa0130450 AT2G02080.1 36.552 145 77 5 107 249 75 206 4.06e-20 90.5
Msa0130450 AT2G02080.4 36.552 145 77 5 107 249 75 206 4.06e-20 90.5
Msa0130450 AT2G02080.5 36.552 145 77 5 107 249 75 206 4.06e-20 90.5
Msa0130450 AT2G02080.3 36.552 145 77 5 107 249 75 206 4.06e-20 90.5
Msa0130450 AT3G45260.2 34.615 156 90 4 109 263 62 206 4.67e-20 90.1
Msa0130450 AT3G45260.1 34.615 156 90 4 109 263 62 206 4.67e-20 90.1
Msa0130450 AT5G60470.1 35.211 142 80 4 109 249 58 188 6.53e-20 89.7
Msa0130450 AT5G60470.3 35.211 142 80 4 109 249 63 193 7.70e-20 89.4
Msa0130450 AT5G60470.2 35.211 142 80 4 109 249 67 197 7.75e-20 89.4
Msa0130450 AT5G66730.1 34.000 150 84 5 107 254 53 189 1.11e-19 89.4
Msa0130450 AT1G14580.1 37.241 145 76 6 107 249 74 205 1.42e-19 89.0
Msa0130450 AT1G14580.2 37.241 145 76 6 107 249 74 205 1.42e-19 89.0
Msa0130450 AT1G14580.3 37.241 145 76 6 107 249 88 219 1.62e-19 88.6
Msa0130450 AT1G03840.2 31.638 177 107 6 83 257 35 199 3.01e-19 87.8
Msa0130450 AT2G02080.6 36.620 142 75 5 110 249 1 129 3.57e-19 87.4
Msa0130450 AT2G02080.2 36.620 142 75 5 110 249 1 129 3.57e-19 87.4
Msa0130450 AT3G50700.1 35.172 145 79 5 107 249 55 186 5.69e-19 87.0
Msa0130450 AT5G44160.2 34.667 150 85 5 109 257 21 158 1.17e-18 85.9
Msa0130450 AT1G03840.1 31.844 179 106 6 83 257 35 201 1.78e-18 85.9
Msa0130450 AT3G13810.4 32.051 156 94 4 107 261 91 235 2.56e-18 85.1
Msa0130450 AT3G13810.1 32.051 156 94 4 107 261 91 235 2.56e-18 85.1
Msa0130450 AT3G13810.5 32.051 156 94 4 107 261 86 230 2.60e-18 85.1
Msa0130450 AT3G13810.2 32.895 152 90 4 107 257 92 232 2.76e-18 85.1
Msa0130450 AT3G13810.6 32.051 156 94 4 107 261 72 216 2.83e-18 85.1
Msa0130450 AT3G13810.3 32.895 152 90 4 107 257 78 218 2.89e-18 85.1
Msa0130450 AT4G02670.1 34.028 144 83 4 107 249 74 206 3.81e-18 84.3
Msa0130450 AT4G02670.2 34.028 144 83 4 107 249 76 208 3.82e-18 84.3

Find 79 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCGAGTTTGGGAGGTATT+TGG 0.247423 1_3:-60634493 None:intergenic
CTCCCATTATTCCTCAATAT+TGG 0.256317 1_3:+60634666 Msa0130450:CDS
AAAGATCACGTTCGTGCTTT+TGG 0.260937 1_3:+60635370 Msa0130450:CDS
AGTGAAAGTTGGATTAATTA+AGG 0.285981 1_3:-60634627 None:intergenic
GATCCAATATTGAGGAATAA+TGG 0.295991 1_3:-60634669 None:intergenic
AAAGGACCGGAGTCTCTAAA+AGG 0.306782 1_3:+60635070 Msa0130450:CDS
GATGAAGATGATGCTGGTTT+TGG 0.319383 1_3:-60635094 None:intergenic
TCTCCTGCTCAAATCCTTGT+TGG 0.331954 1_3:+60634695 Msa0130450:CDS
TTCCTCTCTCTTGCTCCTCC+TGG 0.347551 1_3:+60634467 Msa0130450:CDS
AGAAGAATTGTGGGAAGCTT+TGG 0.351421 1_3:+60635305 Msa0130450:CDS
AATGGGAGTGTGAGATGGTT+TGG 0.355414 1_3:-60634651 None:intergenic
TGCAGACAGTGCAAGAAAAC+TGG 0.374039 1_3:-60634724 None:intergenic
AGGGATAGTAGTAGTAATAG+TGG 0.376112 1_3:-60634525 None:intergenic
AGAGAGGTAGTAGTAGAAAT+AGG 0.389319 1_3:-60634563 None:intergenic
ATCCAATATTGAGGAATAAT+GGG 0.396877 1_3:-60634668 None:intergenic
CTACATTTACAGCACAAGTA+TGG 0.418258 1_3:-60634595 None:intergenic
GATGGTTTGGAAGTGAAAGT+TGG 0.427167 1_3:-60634638 None:intergenic
TCTTTCAGTGGTCTTGCCCT+TGG 0.430630 1_3:-60635175 None:intergenic
CATTTGCTGTGAGAGGAGAT+TGG 0.436076 1_3:+60635272 Msa0130450:CDS
TAGTAGTAATAGTGGGATGA+GGG 0.441652 1_3:-60634517 None:intergenic
TTGGGAGGTATTTGGTGGCC+AGG 0.460715 1_3:-60634485 None:intergenic
GGGGACATGGATCTCAATAT+AGG 0.462797 1_3:+60635047 Msa0130450:CDS
GTAGTAGTAATAGTGGGATG+AGG 0.468089 1_3:-60634518 None:intergenic
GAAATAGGTATTTGATGCAA+GGG 0.489738 1_3:-60634548 None:intergenic
CATGTTGTTGAAACGGTTGA+AGG 0.493614 1_3:-60634753 None:intergenic
AGGAGCAAGAGAGAGGAAGA+TGG 0.499455 1_3:-60634462 None:intergenic
GGGATAGTAGTAGTAATAGT+GGG 0.503268 1_3:-60634524 None:intergenic
CATTCGAGTGTAGGAAGTGT+AGG 0.503643 1_3:+60635245 Msa0130450:CDS
CCATGTTATTGTTGTGCTGA+GGG 0.507339 1_3:+60635130 Msa0130450:CDS
CGAGTTTGGGAGGTATTTGG+TGG 0.508108 1_3:-60634490 None:intergenic
AGAAATAGGTATTTGATGCA+AGG 0.514616 1_3:-60634549 None:intergenic
GGACACATGAGAAGAATTGT+GGG 0.524360 1_3:+60635296 Msa0130450:CDS
ACAAGGATTTGAGCAGGAGA+AGG 0.531708 1_3:-60634692 None:intergenic
CAACCGTTTCAACAACATGC+AGG 0.536317 1_3:+60634757 Msa0130450:CDS
GAAACAACATAAACTATCCA+AGG 0.546681 1_3:+60635158 Msa0130450:CDS
GAACCTGCATGTTGTTGAAA+CGG 0.548476 1_3:-60634760 None:intergenic
AGGACACATGAGAAGAATTG+TGG 0.552345 1_3:+60635295 Msa0130450:CDS
AGCATTGATGAAGATGATGC+TGG 0.557005 1_3:-60635100 None:intergenic
AAGGACCGGAGTCTCTAAAA+GGG 0.557565 1_3:+60635071 Msa0130450:CDS
ATCTAAGCTGCTGCTGCAAG+AGG 0.562751 1_3:-60634429 None:intergenic
TTGTATGGTAGATGCATATG+TGG 0.564484 1_3:+60635026 Msa0130450:intron
TGGCCATGCTCCACACACAT+TGG 0.565061 1_3:+60635396 Msa0130450:CDS
GAGGGAAATCATTCGAGTGT+AGG 0.566094 1_3:+60635236 Msa0130450:CDS
GGAGGTATTTGGTGGCCAGG+AGG 0.567766 1_3:-60634482 None:intergenic
TACATGGAAGAAGAAGAAAG+TGG 0.570369 1_3:+60635457 Msa0130450:CDS
ATAGGTATTTGATGCAAGGG+AGG 0.571468 1_3:-60634545 None:intergenic
GGACCAACAAGGATTTGAGC+AGG 0.575694 1_3:-60634698 None:intergenic
ACACACTACAAAAGAAAGCA+TGG 0.577637 1_3:+60635214 Msa0130450:CDS
CAAGGATTTGAGCAGGAGAA+GGG 0.578543 1_3:-60634691 None:intergenic
TAAAGTAAATGAAAATTACA+TGG 0.583660 1_3:+60635441 Msa0130450:CDS
AGTAGTAATAGTGGGATGAG+GGG 0.585249 1_3:-60634516 None:intergenic
CTCAAAGATTCCAATGTGTG+TGG 0.591809 1_3:-60635406 None:intergenic
GGCCAGGAGGAGCAAGAGAG+AGG 0.593784 1_3:-60634469 None:intergenic
ACCATGTTATTGTTGTGCTG+AGG 0.593810 1_3:+60635129 Msa0130450:CDS
GGAATAATGGGAGTGTGAGA+TGG 0.594723 1_3:-60634656 None:intergenic
TTGCTGTGAGAGGAGATTGG+AGG 0.596970 1_3:+60635275 Msa0130450:CDS
ATCTCAATATAGGAAAGGAC+CGG 0.597133 1_3:+60635057 Msa0130450:CDS
AGGGTTCTGAAATCTTTCAG+TGG 0.606548 1_3:-60635187 None:intergenic
GGAGAAGGGATCCAATATTG+AGG 0.614687 1_3:-60634677 None:intergenic
AGGAAGACATTTGCTGTGAG+AGG 0.616285 1_3:+60635265 Msa0130450:CDS
CATGGATCTCAATATAGGAA+AGG 0.616598 1_3:+60635052 Msa0130450:CDS
TGTATGGTAGATGCATATGT+GGG 0.620648 1_3:+60635027 Msa0130450:intron
CCCTCAGCACAACAATAACA+TGG 0.625348 1_3:-60635130 None:intergenic
GTAGTAATAGTGGGATGAGG+GGG 0.632310 1_3:-60634515 None:intergenic
TAGATGCATATGTGGGGACA+TGG 0.632523 1_3:+60635034 Msa0130450:intron
TCTAAGCTGCTGCTGCAAGA+GGG 0.633302 1_3:-60634428 None:intergenic
AGTATGGTCATGGTGAAGAG+AGG 0.640967 1_3:-60634579 None:intergenic
TAGGTATTTGATGCAAGGGA+GGG 0.641902 1_3:-60634544 None:intergenic
GCAGACAGTGCAAGAAAACT+GGG 0.643201 1_3:-60634723 None:intergenic
ACTACAAAAGAAAGCATGGA+GGG 0.646182 1_3:+60635218 Msa0130450:CDS
AAACAACATAAACTATCCAA+GGG 0.649476 1_3:+60635159 Msa0130450:CDS
TTACAGCACAAGTATGGTCA+TGG 0.652632 1_3:-60634589 None:intergenic
ATTCCAATGTGTGTGGAGCA+TGG 0.657543 1_3:-60635399 None:intergenic
ACAGTGCAAGAAAACTGGGT+GGG 0.660438 1_3:-60634719 None:intergenic
AAAACTGGGTGGGACCAACA+AGG 0.661623 1_3:-60634709 None:intergenic
GTACTGATGATGAGTTTGCA+TGG 0.690956 1_3:-60634384 None:intergenic
GACAGTGCAAGAAAACTGGG+TGG 0.707905 1_3:-60634720 None:intergenic
CACTACAAAAGAAAGCATGG+AGG 0.735997 1_3:+60635217 Msa0130450:CDS
GTATGGTAGATGCATATGTG+GGG 0.763722 1_3:+60635028 Msa0130450:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAATGAATGATTAATTTAA+AGG - chr1_3:60634883-60634902 None:intergenic 15.0%
!! AAACATGATTCATATATATA+TGG + chr1_3:60634981-60635000 Msa0130450:intron 15.0%
!! TATGATATTATTGTATTGTA+TGG + chr1_3:60635011-60635030 Msa0130450:intron 15.0%
!! TAAAGTAAATGAAAATTACA+TGG + chr1_3:60635441-60635460 Msa0130450:CDS 15.0%
!! CTAATGAATGATTAATTTAA+AGG - chr1_3:60634883-60634902 None:intergenic 15.0%
!! AAACATGATTCATATATATA+TGG + chr1_3:60634981-60635000 Msa0130450:intron 15.0%
!! TATGATATTATTGTATTGTA+TGG + chr1_3:60635011-60635030 Msa0130450:intron 15.0%
!! TAAAGTAAATGAAAATTACA+TGG + chr1_3:60635441-60635460 Msa0130450:CDS 15.0%
! AGTGAAAGTTGGATTAATTA+AGG - chr1_3:60634630-60634649 None:intergenic 25.0%
! ATCCAATATTGAGGAATAAT+GGG - chr1_3:60634671-60634690 None:intergenic 25.0%
! GAATGATTAATTTAAAGGCA+AGG - chr1_3:60634878-60634897 None:intergenic 25.0%
! AACTCAATATGATTCTTTCA+TGG - chr1_3:60634945-60634964 None:intergenic 25.0%
! AAACAACATAAACTATCCAA+GGG + chr1_3:60635159-60635178 Msa0130450:CDS 25.0%
! AGTGAAAGTTGGATTAATTA+AGG - chr1_3:60634630-60634649 None:intergenic 25.0%
! ATCCAATATTGAGGAATAAT+GGG - chr1_3:60634671-60634690 None:intergenic 25.0%
! GAATGATTAATTTAAAGGCA+AGG - chr1_3:60634878-60634897 None:intergenic 25.0%
! AACTCAATATGATTCTTTCA+TGG - chr1_3:60634945-60634964 None:intergenic 25.0%
! AAACAACATAAACTATCCAA+GGG + chr1_3:60635159-60635178 Msa0130450:CDS 25.0%
GAAATAGGTATTTGATGCAA+GGG - chr1_3:60634551-60634570 None:intergenic 30.0%
AGAAATAGGTATTTGATGCA+AGG - chr1_3:60634552-60634571 None:intergenic 30.0%
GATCCAATATTGAGGAATAA+TGG - chr1_3:60634672-60634691 None:intergenic 30.0%
TCTTTCATGGAAGAAAAGTA+AGG - chr1_3:60634932-60634951 None:intergenic 30.0%
GAAACAACATAAACTATCCA+AGG + chr1_3:60635158-60635177 Msa0130450:CDS 30.0%
! TCTTTTGTAGTGTGTTTGAA+GGG - chr1_3:60635209-60635228 None:intergenic 30.0%
! TTCTTTTGTAGTGTGTTTGA+AGG - chr1_3:60635210-60635229 None:intergenic 30.0%
!! TTGGAATCTTTGAGTTTTGA+TGG + chr1_3:60635415-60635434 Msa0130450:CDS 30.0%
GAAATAGGTATTTGATGCAA+GGG - chr1_3:60634551-60634570 None:intergenic 30.0%
AGAAATAGGTATTTGATGCA+AGG - chr1_3:60634552-60634571 None:intergenic 30.0%
GATCCAATATTGAGGAATAA+TGG - chr1_3:60634672-60634691 None:intergenic 30.0%
TCTTTCATGGAAGAAAAGTA+AGG - chr1_3:60634932-60634951 None:intergenic 30.0%
GAAACAACATAAACTATCCA+AGG + chr1_3:60635158-60635177 Msa0130450:CDS 30.0%
! TCTTTTGTAGTGTGTTTGAA+GGG - chr1_3:60635209-60635228 None:intergenic 30.0%
! TTCTTTTGTAGTGTGTTTGA+AGG - chr1_3:60635210-60635229 None:intergenic 30.0%
!! TTGGAATCTTTGAGTTTTGA+TGG + chr1_3:60635415-60635434 Msa0130450:CDS 30.0%
! TAGTAGTAATAGTGGGATGA+GGG - chr1_3:60634520-60634539 None:intergenic 35.0%
GGGATAGTAGTAGTAATAGT+GGG - chr1_3:60634527-60634546 None:intergenic 35.0%
AGGGATAGTAGTAGTAATAG+TGG - chr1_3:60634528-60634547 None:intergenic 35.0%
AGAGAGGTAGTAGTAGAAAT+AGG - chr1_3:60634566-60634585 None:intergenic 35.0%
CTACATTTACAGCACAAGTA+TGG - chr1_3:60634598-60634617 None:intergenic 35.0%
CTCCCATTATTCCTCAATAT+TGG + chr1_3:60634666-60634685 Msa0130450:CDS 35.0%
TTGTATGGTAGATGCATATG+TGG + chr1_3:60635026-60635045 Msa0130450:intron 35.0%
TGTATGGTAGATGCATATGT+GGG + chr1_3:60635027-60635046 Msa0130450:intron 35.0%
CATGGATCTCAATATAGGAA+AGG + chr1_3:60635052-60635071 Msa0130450:CDS 35.0%
ATCTCAATATAGGAAAGGAC+CGG + chr1_3:60635057-60635076 Msa0130450:CDS 35.0%
ACACACTACAAAAGAAAGCA+TGG + chr1_3:60635214-60635233 Msa0130450:CDS 35.0%
! ACTACAAAAGAAAGCATGGA+GGG + chr1_3:60635218-60635237 Msa0130450:CDS 35.0%
!!! AAGCTTTGGTTTTGTATCTG+TGG + chr1_3:60635319-60635338 Msa0130450:CDS 35.0%
TACATGGAAGAAGAAGAAAG+TGG + chr1_3:60635457-60635476 Msa0130450:CDS 35.0%
! TAGTAGTAATAGTGGGATGA+GGG - chr1_3:60634520-60634539 None:intergenic 35.0%
GGGATAGTAGTAGTAATAGT+GGG - chr1_3:60634527-60634546 None:intergenic 35.0%
AGGGATAGTAGTAGTAATAG+TGG - chr1_3:60634528-60634547 None:intergenic 35.0%
AGAGAGGTAGTAGTAGAAAT+AGG - chr1_3:60634566-60634585 None:intergenic 35.0%
CTACATTTACAGCACAAGTA+TGG - chr1_3:60634598-60634617 None:intergenic 35.0%
CTCCCATTATTCCTCAATAT+TGG + chr1_3:60634666-60634685 Msa0130450:CDS 35.0%
TTGTATGGTAGATGCATATG+TGG + chr1_3:60635026-60635045 Msa0130450:intron 35.0%
TGTATGGTAGATGCATATGT+GGG + chr1_3:60635027-60635046 Msa0130450:intron 35.0%
CATGGATCTCAATATAGGAA+AGG + chr1_3:60635052-60635071 Msa0130450:CDS 35.0%
ATCTCAATATAGGAAAGGAC+CGG + chr1_3:60635057-60635076 Msa0130450:CDS 35.0%
ACACACTACAAAAGAAAGCA+TGG + chr1_3:60635214-60635233 Msa0130450:CDS 35.0%
! ACTACAAAAGAAAGCATGGA+GGG + chr1_3:60635218-60635237 Msa0130450:CDS 35.0%
!!! AAGCTTTGGTTTTGTATCTG+TGG + chr1_3:60635319-60635338 Msa0130450:CDS 35.0%
TACATGGAAGAAGAAGAAAG+TGG + chr1_3:60635457-60635476 Msa0130450:CDS 35.0%
! AGTAGTAATAGTGGGATGAG+GGG - chr1_3:60634519-60634538 None:intergenic 40.0%
! GTAGTAGTAATAGTGGGATG+AGG - chr1_3:60634521-60634540 None:intergenic 40.0%
TAGGTATTTGATGCAAGGGA+GGG - chr1_3:60634547-60634566 None:intergenic 40.0%
ATAGGTATTTGATGCAAGGG+AGG - chr1_3:60634548-60634567 None:intergenic 40.0%
TTACAGCACAAGTATGGTCA+TGG - chr1_3:60634592-60634611 None:intergenic 40.0%
! GATGGTTTGGAAGTGAAAGT+TGG - chr1_3:60634641-60634660 None:intergenic 40.0%
CATGTTGTTGAAACGGTTGA+AGG - chr1_3:60634756-60634775 None:intergenic 40.0%
GAACCTGCATGTTGTTGAAA+CGG - chr1_3:60634763-60634782 None:intergenic 40.0%
GTATGGTAGATGCATATGTG+GGG + chr1_3:60635028-60635047 Msa0130450:intron 40.0%
!!! GATGAAGATGATGCTGGTTT+TGG - chr1_3:60635097-60635116 None:intergenic 40.0%
!! AGCATTGATGAAGATGATGC+TGG - chr1_3:60635103-60635122 None:intergenic 40.0%
ACCATGTTATTGTTGTGCTG+AGG + chr1_3:60635129-60635148 Msa0130450:CDS 40.0%
CCATGTTATTGTTGTGCTGA+GGG + chr1_3:60635130-60635149 Msa0130450:CDS 40.0%
!! AGGGTTCTGAAATCTTTCAG+TGG - chr1_3:60635190-60635209 None:intergenic 40.0%
! CACTACAAAAGAAAGCATGG+AGG + chr1_3:60635217-60635236 Msa0130450:CDS 40.0%
AGGACACATGAGAAGAATTG+TGG + chr1_3:60635295-60635314 Msa0130450:CDS 40.0%
GGACACATGAGAAGAATTGT+GGG + chr1_3:60635296-60635315 Msa0130450:CDS 40.0%
AGAAGAATTGTGGGAAGCTT+TGG + chr1_3:60635305-60635324 Msa0130450:CDS 40.0%
! AAAGATCACGTTCGTGCTTT+TGG + chr1_3:60635370-60635389 Msa0130450:CDS 40.0%
CTCAAAGATTCCAATGTGTG+TGG - chr1_3:60635409-60635428 None:intergenic 40.0%
! AGTAGTAATAGTGGGATGAG+GGG - chr1_3:60634519-60634538 None:intergenic 40.0%
! GTAGTAGTAATAGTGGGATG+AGG - chr1_3:60634521-60634540 None:intergenic 40.0%
TAGGTATTTGATGCAAGGGA+GGG - chr1_3:60634547-60634566 None:intergenic 40.0%
ATAGGTATTTGATGCAAGGG+AGG - chr1_3:60634548-60634567 None:intergenic 40.0%
TTACAGCACAAGTATGGTCA+TGG - chr1_3:60634592-60634611 None:intergenic 40.0%
! GATGGTTTGGAAGTGAAAGT+TGG - chr1_3:60634641-60634660 None:intergenic 40.0%
CATGTTGTTGAAACGGTTGA+AGG - chr1_3:60634756-60634775 None:intergenic 40.0%
GAACCTGCATGTTGTTGAAA+CGG - chr1_3:60634763-60634782 None:intergenic 40.0%
GTATGGTAGATGCATATGTG+GGG + chr1_3:60635028-60635047 Msa0130450:intron 40.0%
!!! GATGAAGATGATGCTGGTTT+TGG - chr1_3:60635097-60635116 None:intergenic 40.0%
!! AGCATTGATGAAGATGATGC+TGG - chr1_3:60635103-60635122 None:intergenic 40.0%
ACCATGTTATTGTTGTGCTG+AGG + chr1_3:60635129-60635148 Msa0130450:CDS 40.0%
CCATGTTATTGTTGTGCTGA+GGG + chr1_3:60635130-60635149 Msa0130450:CDS 40.0%
!! AGGGTTCTGAAATCTTTCAG+TGG - chr1_3:60635190-60635209 None:intergenic 40.0%
! CACTACAAAAGAAAGCATGG+AGG + chr1_3:60635217-60635236 Msa0130450:CDS 40.0%
AGGACACATGAGAAGAATTG+TGG + chr1_3:60635295-60635314 Msa0130450:CDS 40.0%
GGACACATGAGAAGAATTGT+GGG + chr1_3:60635296-60635315 Msa0130450:CDS 40.0%
AGAAGAATTGTGGGAAGCTT+TGG + chr1_3:60635305-60635324 Msa0130450:CDS 40.0%
! AAAGATCACGTTCGTGCTTT+TGG + chr1_3:60635370-60635389 Msa0130450:CDS 40.0%
CTCAAAGATTCCAATGTGTG+TGG - chr1_3:60635409-60635428 None:intergenic 40.0%
! GTAGTAATAGTGGGATGAGG+GGG - chr1_3:60634518-60634537 None:intergenic 45.0%
AGTATGGTCATGGTGAAGAG+AGG - chr1_3:60634582-60634601 None:intergenic 45.0%
! AATGGGAGTGTGAGATGGTT+TGG - chr1_3:60634654-60634673 None:intergenic 45.0%
! GGAATAATGGGAGTGTGAGA+TGG - chr1_3:60634659-60634678 None:intergenic 45.0%
GGAGAAGGGATCCAATATTG+AGG - chr1_3:60634680-60634699 None:intergenic 45.0%
CAAGGATTTGAGCAGGAGAA+GGG - chr1_3:60634694-60634713 None:intergenic 45.0%
ACAAGGATTTGAGCAGGAGA+AGG - chr1_3:60634695-60634714 None:intergenic 45.0%
TCTCCTGCTCAAATCCTTGT+TGG + chr1_3:60634695-60634714 Msa0130450:CDS 45.0%
ACAGTGCAAGAAAACTGGGT+GGG - chr1_3:60634722-60634741 None:intergenic 45.0%
GCAGACAGTGCAAGAAAACT+GGG - chr1_3:60634726-60634745 None:intergenic 45.0%
TGCAGACAGTGCAAGAAAAC+TGG - chr1_3:60634727-60634746 None:intergenic 45.0%
CAACCGTTTCAACAACATGC+AGG + chr1_3:60634757-60634776 Msa0130450:CDS 45.0%
TAGATGCATATGTGGGGACA+TGG + chr1_3:60635034-60635053 Msa0130450:intron 45.0%
GGGGACATGGATCTCAATAT+AGG + chr1_3:60635047-60635066 Msa0130450:CDS 45.0%
AAAGGACCGGAGTCTCTAAA+AGG + chr1_3:60635070-60635089 Msa0130450:CDS 45.0%
! AAGGACCGGAGTCTCTAAAA+GGG + chr1_3:60635071-60635090 Msa0130450:CDS 45.0%
!! TTTGGCCCTTTTAGAGACTC+CGG - chr1_3:60635079-60635098 None:intergenic 45.0%
CCCTCAGCACAACAATAACA+TGG - chr1_3:60635133-60635152 None:intergenic 45.0%
GAGGGAAATCATTCGAGTGT+AGG + chr1_3:60635236-60635255 Msa0130450:CDS 45.0%
! CATTCGAGTGTAGGAAGTGT+AGG + chr1_3:60635245-60635264 Msa0130450:CDS 45.0%
!! AGGAAGACATTTGCTGTGAG+AGG + chr1_3:60635265-60635284 Msa0130450:CDS 45.0%
!! CATTTGCTGTGAGAGGAGAT+TGG + chr1_3:60635272-60635291 Msa0130450:CDS 45.0%
! CACGTTCGTGCTTTTGGTAA+TGG + chr1_3:60635376-60635395 Msa0130450:CDS 45.0%
ATTCCAATGTGTGTGGAGCA+TGG - chr1_3:60635402-60635421 None:intergenic 45.0%
! GTAGTAATAGTGGGATGAGG+GGG - chr1_3:60634518-60634537 None:intergenic 45.0%
AGTATGGTCATGGTGAAGAG+AGG - chr1_3:60634582-60634601 None:intergenic 45.0%
! AATGGGAGTGTGAGATGGTT+TGG - chr1_3:60634654-60634673 None:intergenic 45.0%
! GGAATAATGGGAGTGTGAGA+TGG - chr1_3:60634659-60634678 None:intergenic 45.0%
GGAGAAGGGATCCAATATTG+AGG - chr1_3:60634680-60634699 None:intergenic 45.0%
CAAGGATTTGAGCAGGAGAA+GGG - chr1_3:60634694-60634713 None:intergenic 45.0%
ACAAGGATTTGAGCAGGAGA+AGG - chr1_3:60634695-60634714 None:intergenic 45.0%
TCTCCTGCTCAAATCCTTGT+TGG + chr1_3:60634695-60634714 Msa0130450:CDS 45.0%
ACAGTGCAAGAAAACTGGGT+GGG - chr1_3:60634722-60634741 None:intergenic 45.0%
GCAGACAGTGCAAGAAAACT+GGG - chr1_3:60634726-60634745 None:intergenic 45.0%
TGCAGACAGTGCAAGAAAAC+TGG - chr1_3:60634727-60634746 None:intergenic 45.0%
CAACCGTTTCAACAACATGC+AGG + chr1_3:60634757-60634776 Msa0130450:CDS 45.0%
TAGATGCATATGTGGGGACA+TGG + chr1_3:60635034-60635053 Msa0130450:intron 45.0%
GGGGACATGGATCTCAATAT+AGG + chr1_3:60635047-60635066 Msa0130450:CDS 45.0%
AAAGGACCGGAGTCTCTAAA+AGG + chr1_3:60635070-60635089 Msa0130450:CDS 45.0%
! AAGGACCGGAGTCTCTAAAA+GGG + chr1_3:60635071-60635090 Msa0130450:CDS 45.0%
!! TTTGGCCCTTTTAGAGACTC+CGG - chr1_3:60635079-60635098 None:intergenic 45.0%
CCCTCAGCACAACAATAACA+TGG - chr1_3:60635133-60635152 None:intergenic 45.0%
GAGGGAAATCATTCGAGTGT+AGG + chr1_3:60635236-60635255 Msa0130450:CDS 45.0%
! CATTCGAGTGTAGGAAGTGT+AGG + chr1_3:60635245-60635264 Msa0130450:CDS 45.0%
!! AGGAAGACATTTGCTGTGAG+AGG + chr1_3:60635265-60635284 Msa0130450:CDS 45.0%
!! CATTTGCTGTGAGAGGAGAT+TGG + chr1_3:60635272-60635291 Msa0130450:CDS 45.0%
! CACGTTCGTGCTTTTGGTAA+TGG + chr1_3:60635376-60635395 Msa0130450:CDS 45.0%
ATTCCAATGTGTGTGGAGCA+TGG - chr1_3:60635402-60635421 None:intergenic 45.0%
!! AAATGATAAAAATATAATAA+TGG - chr1_3:60634788-60634807 None:intergenic 5.0%
!! AAATGATAAAAATATAATAA+TGG - chr1_3:60634788-60634807 None:intergenic 5.0%
TCTAAGCTGCTGCTGCAAGA+GGG - chr1_3:60634431-60634450 None:intergenic 50.0%
ATCTAAGCTGCTGCTGCAAG+AGG - chr1_3:60634432-60634451 None:intergenic 50.0%
AGGAGCAAGAGAGAGGAAGA+TGG - chr1_3:60634465-60634484 None:intergenic 50.0%
!! CGAGTTTGGGAGGTATTTGG+TGG - chr1_3:60634493-60634512 None:intergenic 50.0%
!! GTGCGAGTTTGGGAGGTATT+TGG - chr1_3:60634496-60634515 None:intergenic 50.0%
GGACCAACAAGGATTTGAGC+AGG - chr1_3:60634701-60634720 None:intergenic 50.0%
AAAACTGGGTGGGACCAACA+AGG - chr1_3:60634712-60634731 None:intergenic 50.0%
GACAGTGCAAGAAAACTGGG+TGG - chr1_3:60634723-60634742 None:intergenic 50.0%
! TCTTTCAGTGGTCTTGCCCT+TGG - chr1_3:60635178-60635197 None:intergenic 50.0%
TTGCTGTGAGAGGAGATTGG+AGG + chr1_3:60635275-60635294 Msa0130450:CDS 50.0%
TCTAAGCTGCTGCTGCAAGA+GGG - chr1_3:60634431-60634450 None:intergenic 50.0%
ATCTAAGCTGCTGCTGCAAG+AGG - chr1_3:60634432-60634451 None:intergenic 50.0%
AGGAGCAAGAGAGAGGAAGA+TGG - chr1_3:60634465-60634484 None:intergenic 50.0%
!! CGAGTTTGGGAGGTATTTGG+TGG - chr1_3:60634493-60634512 None:intergenic 50.0%
!! GTGCGAGTTTGGGAGGTATT+TGG - chr1_3:60634496-60634515 None:intergenic 50.0%
GGACCAACAAGGATTTGAGC+AGG - chr1_3:60634701-60634720 None:intergenic 50.0%
AAAACTGGGTGGGACCAACA+AGG - chr1_3:60634712-60634731 None:intergenic 50.0%
GACAGTGCAAGAAAACTGGG+TGG - chr1_3:60634723-60634742 None:intergenic 50.0%
! TCTTTCAGTGGTCTTGCCCT+TGG - chr1_3:60635178-60635197 None:intergenic 50.0%
TTGCTGTGAGAGGAGATTGG+AGG + chr1_3:60635275-60635294 Msa0130450:CDS 50.0%
TTCCTCTCTCTTGCTCCTCC+TGG + chr1_3:60634467-60634486 Msa0130450:CDS 55.0%
!! TTGGGAGGTATTTGGTGGCC+AGG - chr1_3:60634488-60634507 None:intergenic 55.0%
TGGCCATGCTCCACACACAT+TGG + chr1_3:60635396-60635415 Msa0130450:CDS 55.0%
TTCCTCTCTCTTGCTCCTCC+TGG + chr1_3:60634467-60634486 Msa0130450:CDS 55.0%
!! TTGGGAGGTATTTGGTGGCC+AGG - chr1_3:60634488-60634507 None:intergenic 55.0%
TGGCCATGCTCCACACACAT+TGG + chr1_3:60635396-60635415 Msa0130450:CDS 55.0%
!! GGAGGTATTTGGTGGCCAGG+AGG - chr1_3:60634485-60634504 None:intergenic 60.0%
! GGATGAGGGGGTGCGAGTTT+GGG - chr1_3:60634506-60634525 None:intergenic 60.0%
!! GGAGGTATTTGGTGGCCAGG+AGG - chr1_3:60634485-60634504 None:intergenic 60.0%
! GGATGAGGGGGTGCGAGTTT+GGG - chr1_3:60634506-60634525 None:intergenic 60.0%
GGCCAGGAGGAGCAAGAGAG+AGG - chr1_3:60634472-60634491 None:intergenic 65.0%
! TGAGGGGGTGCGAGTTTGGG+AGG - chr1_3:60634503-60634522 None:intergenic 65.0%
! GGGATGAGGGGGTGCGAGTT+TGG - chr1_3:60634507-60634526 None:intergenic 65.0%
GGCCAGGAGGAGCAAGAGAG+AGG - chr1_3:60634472-60634491 None:intergenic 65.0%
! TGAGGGGGTGCGAGTTTGGG+AGG - chr1_3:60634503-60634522 None:intergenic 65.0%
! GGGATGAGGGGGTGCGAGTT+TGG - chr1_3:60634507-60634526 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 60634386 60635528 60634386 ID=Msa0130450;Name=Msa0130450
chr1_3 mRNA 60634386 60635528 60634386 ID=Msa0130450-mRNA-1;Parent=Msa0130450;Name=Msa0130450-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|0|0|1|1|1|2|0|294
chr1_3 exon 60634386 60634778 60634386 ID=Msa0130450-mRNA-1:exon:20003;Parent=Msa0130450-mRNA-1
chr1_3 exon 60635037 60635528 60635037 ID=Msa0130450-mRNA-1:exon:20004;Parent=Msa0130450-mRNA-1
chr1_3 CDS 60634386 60634778 60634386 ID=Msa0130450-mRNA-1:cds;Parent=Msa0130450-mRNA-1
chr1_3 CDS 60635037 60635528 60635037 ID=Msa0130450-mRNA-1:cds;Parent=Msa0130450-mRNA-1
Gene Sequence

>Msa0130450

ATGCAAACTCATCATCAGTACTACTCATTCTCTTGTGAGAGTCCCTCTTGCAGCAGCAGCTTAGATGATGAAGATTCCATCTTCCTCTCTCTTGCTCCTCCTGGCCACCAAATACCTCCCAAACTCGCACCCCCTCATCCCACTATTACTACTACTATCCCTCCCTTGCATCAAATACCTATTTCTACTACTACCTCTCTTCACCATGACCATACTTGTGCTGTAAATGTAGCACTTAATGCCTTAATTAATCCAACTTTCACTTCCAAACCATCTCACACTCCCATTATTCCTCAATATTGGATCCCTTCTCCTGCTCAAATCCTTGTTGGTCCCACCCAGTTTTCTTGCACTGTCTGCAACAAAACCTTCAACCGTTTCAACAACATGCAGATGCATATGTGGGGACATGGATCTCAATATAGGAAAGGACCGGAGTCTCTAAAAGGGCCAAAACCAGCATCATCTTCATCAATGCTGAAACTACCATGTTATTGTTGTGCTGAGGGTTGCAGAAACAACATAAACTATCCAAGGGCAAGACCACTGAAAGATTTCAGAACCCTTCAAACACACTACAAAAGAAAGCATGGAGGGAAATCATTCGAGTGTAGGAAGTGTAGGAAGACATTTGCTGTGAGAGGAGATTGGAGGACACATGAGAAGAATTGTGGGAAGCTTTGGTTTTGTATCTGTGGTTCTGATTTCAAACACAAAAGATCACTAAAAGATCACGTTCGTGCTTTTGGTAATGGCCATGCTCCACACACATTGGAATCTTTGAGTTTTGATGGACATAAAGTAAATGAAAATTACATGGAAGAAGAAGAAAGTGGAAGTGAAGATGAAGACAATAACAGTGAAGTTGTTTTTAGTTTGAATTAA
Protein sequence

>Msa0130450

MQTHHQYYSFSCESPSCSSSLDDEDSIFLSLAPPGHQIPPKLAPPHPTITTTIPPLHQIPISTTTSLHHDHTCAVNVALNALINPTFTSKPSHTPIIPQYWIPSPAQILVGPTQFSCTVCNKTFNRFNNMQMHMWGHGSQYRKGPESLKGPKPASSSSMLKLPCYCCAEGCRNNINYPRARPLKDFRTLQTHYKRKHGGKSFECRKCRKTFAVRGDWRTHEKNCGKLWFCICGSDFKHKRSLKDHVRAFGNGHAPHTLESLSFDGHKVNENYMEEEESGSEDEDNNSEVVFSLN*