AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0131060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0131060 MtrunA17_Chr1g0199681 97.115 624 12 2 1 623 76 694 0.0 1256
Msa0131060 MtrunA17_Chr2g0326761 57.867 572 200 11 10 573 2 540 0.0 608
Msa0131060 MtrunA17_Chr4g0028971 57.931 580 207 16 8 575 1 555 0.0 568
Msa0131060 MtrunA17_Chr1g0181211 52.117 614 216 16 1 577 1 573 0.0 560
Msa0131060 MtrunA17_Chr7g0264171 48.714 622 259 18 10 612 22 602 9.88e-176 512
Msa0131060 MtrunA17_Chr7g0264161 54.572 339 112 4 28 362 1 301 3.35e-124 369
Msa0131060 MtrunA17_Chr3g0115271 37.742 620 280 18 16 594 16 570 4.37e-113 350
Msa0131060 MtrunA17_Chr5g0424931 37.457 582 270 22 16 578 16 522 1.12e-98 311
Msa0131060 MtrunA17_Chr5g0417281 37.155 471 218 13 11 463 3 413 1.28e-85 277
Msa0131060 MtrunA17_Chr5g0433301 39.130 391 191 8 9 390 14 366 4.56e-80 270
Msa0131060 MtrunA17_Chr2g0282961 39.314 379 177 8 15 382 51 387 2.09e-79 263
Msa0131060 MtrunA17_Chr4g0070181 39.737 380 182 9 13 384 17 357 2.13e-79 272
Msa0131060 MtrunA17_Chr2g0285461 37.956 411 209 8 9 411 13 385 3.80e-78 266
Msa0131060 MtrunA17_Chr5g0436761 35.294 476 231 13 8 472 1 410 1.03e-77 254
Msa0131060 MtrunA17_Chr3g0109391 38.760 387 190 8 13 390 20 368 3.56e-77 262
Msa0131060 MtrunA17_Chr2g0289221 39.286 392 170 10 16 392 17 355 4.05e-77 249
Msa0131060 MtrunA17_Chr8g0376771 37.561 410 211 8 9 411 13 384 1.07e-76 262
Msa0131060 MtrunA17_Chr4g0046371 39.687 383 180 10 12 382 33 376 1.39e-76 258
Msa0131060 MtrunA17_Chr1g0178011 36.842 399 200 8 14 406 7 359 2.85e-76 250
Msa0131060 MtrunA17_Chr1g0156321 38.847 399 192 11 14 402 38 394 2.76e-74 254
Msa0131060 MtrunA17_Chr2g0301251 38.360 378 188 8 14 384 18 357 4.31e-72 251
Msa0131060 MtrunA17_Chr8g0385791 37.313 402 204 8 10 403 50 411 6.51e-72 248
Msa0131060 MtrunA17_Chr5g0417211 38.978 372 156 9 16 372 9 324 1.38e-70 234
Msa0131060 MtrunA17_Chr4g0029671 37.530 413 180 10 19 403 54 416 2.48e-70 243
Msa0131060 MtrunA17_Chr8g0389081 38.501 387 189 8 9 385 22 369 9.62e-70 245
Msa0131060 MtrunA17_Chr2g0277461 37.280 397 188 14 19 406 13 357 5.40e-69 231
Msa0131060 MtrunA17_Chr2g0326281 37.192 406 183 9 19 392 56 421 1.05e-68 239
Msa0131060 MtrunA17_Chr4g0009751 37.750 400 195 11 15 403 15 371 9.36e-68 232
Msa0131060 MtrunA17_Chr2g0276781 36.709 395 196 7 11 394 15 366 2.21e-67 232
Msa0131060 MtrunA17_Chr7g0239061 35.697 409 206 12 8 404 3 366 3.24e-61 216
Msa0131060 MtrunA17_Chr8g0346711 37.531 397 196 10 16 403 26 379 1.54e-60 214
Msa0131060 MtrunA17_Chr5g0425261 35.509 383 186 15 16 388 9 340 1.53e-55 192
Msa0131060 MtrunA17_Chr5g0424801 34.465 383 181 14 16 383 9 336 4.99e-55 191
Msa0131060 MtrunA17_Chr2g0277531 33.708 356 167 12 19 362 20 318 1.37e-51 180
Msa0131060 MtrunA17_Chr5g0425661 41.500 200 96 4 18 206 36 225 2.12e-46 164
Msa0131060 MtrunA17_Chr5g0424741 30.556 504 248 20 16 460 9 469 3.22e-46 171
Msa0131060 MtrunA17_Chr8g0356211 31.949 313 162 8 126 438 2 263 2.11e-37 141
Msa0131060 MtrunA17_Chr5g0425651 41.739 115 60 3 276 386 1 112 8.02e-20 85.5
Msa0131060 MtrunA17_Chr5g0424791 34.868 152 88 4 63 214 11 151 8.56e-20 86.7
Msa0131060 MtrunA17_Chr1g0178071 32.099 162 88 5 113 271 71 213 1.29e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0131060 AT4G30080.1 53.635 619 225 18 1 602 1 574 0.0 579
Msa0131060 AT2G28350.2 55.613 579 197 16 13 566 4 547 0.0 562
Msa0131060 AT2G28350.1 55.613 579 197 16 13 566 4 547 0.0 562
Msa0131060 AT1G77850.1 38.235 612 303 17 16 598 16 581 4.11e-117 361
Msa0131060 AT1G77850.2 38.235 612 303 17 16 598 16 581 4.66e-117 361
Msa0131060 AT5G37020.2 38.760 387 190 8 13 390 16 364 5.39e-79 266
Msa0131060 AT5G37020.1 38.760 387 190 8 13 390 16 364 1.17e-78 266
Msa0131060 AT1G30330.3 38.369 417 208 10 9 415 14 391 2.23e-77 265
Msa0131060 AT1G30330.2 38.369 417 208 10 9 415 14 391 2.23e-77 265
Msa0131060 AT1G30330.1 37.864 412 208 9 13 415 17 389 4.31e-77 264
Msa0131060 AT1G19850.2 39.221 385 179 9 11 382 35 377 2.73e-74 255
Msa0131060 AT1G19220.1 38.084 407 195 12 13 408 17 377 3.92e-74 258
Msa0131060 AT1G19850.1 39.221 385 179 9 11 382 46 388 4.06e-74 255
Msa0131060 AT2G33860.1 38.481 395 194 9 15 400 52 406 9.50e-74 248
Msa0131060 AT5G60450.2 36.496 411 202 7 8 400 30 399 1.51e-73 251
Msa0131060 AT5G60450.1 36.908 401 194 7 18 400 64 423 1.98e-72 248
Msa0131060 AT5G62000.2 38.000 400 198 8 13 403 55 413 4.19e-71 246
Msa0131060 AT5G62000.5 38.000 400 198 8 13 403 55 413 4.19e-71 246
Msa0131060 AT5G62000.3 38.000 400 198 8 13 403 55 413 4.19e-71 246
Msa0131060 AT5G62000.1 38.000 400 198 8 13 403 55 413 4.19e-71 246
Msa0131060 AT5G62000.4 38.000 400 198 8 13 403 55 413 4.58e-71 246
Msa0131060 AT5G20730.2 33.270 526 272 16 13 506 18 496 4.32e-70 246
Msa0131060 AT5G20730.1 33.207 527 272 17 13 506 18 497 8.63e-69 243
Msa0131060 AT5G20730.3 33.207 527 272 17 13 506 18 497 9.70e-69 243
Msa0131060 AT1G59750.4 37.940 398 198 8 15 403 18 375 6.39e-67 231
Msa0131060 AT1G59750.2 37.940 398 198 8 15 403 18 375 7.50e-67 231
Msa0131060 AT1G59750.1 37.940 398 198 8 15 403 18 375 8.00e-67 231
Msa0131060 AT1G59750.3 37.940 398 198 8 15 403 18 375 8.00e-67 231
Msa0131060 AT2G46530.1 39.050 379 173 13 16 382 18 350 1.20e-65 226
Msa0131060 AT2G46530.3 39.050 379 173 13 16 382 39 371 2.57e-65 226
Msa0131060 AT3G61830.1 36.520 408 198 11 7 403 13 370 6.01e-65 224
Msa0131060 AT4G23980.2 36.908 401 201 11 16 407 9 366 7.40e-64 222
Msa0131060 AT4G23980.1 36.908 401 201 11 16 407 9 366 9.50e-64 222
Msa0131060 AT5G37020.3 37.785 307 151 6 86 390 1 269 4.98e-55 199
Msa0131060 AT1G35540.1 35.385 390 202 9 12 395 20 365 8.95e-55 197
Msa0131060 AT1G35520.1 35.733 389 194 11 12 392 20 360 1.83e-54 196
Msa0131060 AT1G34310.1 36.126 382 188 10 19 392 27 360 1.86e-54 196
Msa0131060 AT1G35240.1 35.263 380 190 11 12 382 20 352 8.34e-54 194
Msa0131060 AT1G34390.1 34.129 419 209 15 12 421 20 380 1.73e-53 193
Msa0131060 AT3G61830.2 37.356 348 164 10 60 403 22 319 2.32e-53 192
Msa0131060 AT2G33860.2 39.437 284 135 5 118 400 2 249 6.34e-53 188
Msa0131060 AT1G34170.1 33.333 393 204 12 19 401 25 369 4.86e-52 187
Msa0131060 AT1G34410.1 34.828 379 193 10 12 382 20 352 1.72e-51 187
Msa0131060 AT2G46530.2 40.520 269 116 8 117 382 36 263 5.13e-48 176
Msa0131060 AT1G34170.2 31.807 393 184 12 19 401 25 343 1.01e-44 166
Msa0131060 AT1G34170.3 31.807 393 184 12 19 401 25 343 6.37e-44 165
Msa0131060 AT1G43950.1 40.654 214 108 7 12 217 20 222 3.08e-38 141
Msa0131060 ATMG00940.1 38.365 159 81 6 205 357 1 148 5.73e-22 93.6
Msa0131060 AT2G36080.3 35.714 112 66 3 112 218 32 142 1.43e-11 65.1
Msa0131060 AT2G36080.1 34.234 111 69 2 112 218 32 142 1.51e-11 65.5
Msa0131060 AT2G36080.4 34.234 111 69 2 112 218 32 142 2.64e-11 63.2
Msa0131060 AT2G36080.2 34.234 111 69 2 112 218 32 142 2.64e-11 63.2
Msa0131060 AT3G25730.1 32.824 131 76 2 118 246 183 303 4.23e-11 65.1
Msa0131060 AT2G46870.1 34.906 106 62 2 110 215 27 125 6.60e-11 64.3

Find 145 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTAACTTGTACTTGTTTA+AGG 0.096314 1_3:+61637484 Msa0131060:three_prime_UTR
TTCTAGGATTAGTTGGTTTA+TGG 0.157206 1_3:+61635743 Msa0131060:CDS
TTTGAAGAAGATTGTTTCTT+TGG 0.183552 1_3:+61635042 Msa0131060:CDS
ATAAACCTTGGTTTCTTTCT+TGG 0.198649 1_3:-61636580 None:intergenic
AAATCAGATGGATTATTATT+TGG 0.221410 1_3:-61636781 None:intergenic
ACGTAGGCTTCATCAGTTTC+AGG 0.222422 1_3:-61634979 None:intergenic
ACAAAATATGGTTATGGTTA+TGG 0.233884 1_3:-61637023 None:intergenic
GAGATCTTTGTGTTGGTATA+AGG 0.249926 1_3:+61635379 Msa0131060:CDS
GAGAGGTGGAACACGGGTTT+TGG 0.257788 1_3:-61634917 None:intergenic
TATTGCATAATTCTTCTATT+AGG 0.266082 1_3:+61637356 Msa0131060:three_prime_UTR
CTACAACAAGACATGTTTCT+TGG 0.267352 1_3:+61636823 Msa0131060:CDS
TTCTCCTTGGCGCCTTCTTC+AGG 0.267828 1_3:+61635812 Msa0131060:CDS
TTGTTGAGTGTGGGAAATTC+TGG 0.279840 1_3:+61636961 Msa0131060:CDS
TTGTTTGGTGGTGGTGGTTC+TGG 0.287312 1_3:+61635480 Msa0131060:CDS
CAAAATATGGTTATGGTTAT+GGG 0.304272 1_3:-61637022 None:intergenic
GGAGCCAGGCATGCTCAATT+TGG 0.304628 1_3:+61636757 Msa0131060:CDS
TTGAAACTGAGGATTCTTCT+AGG 0.307810 1_3:+61635727 Msa0131060:CDS
ATTGTTATTGATACTCTTGT+AGG 0.312189 1_3:-61636905 None:intergenic
TCTAGGATTAGTTGGTTTAT+GGG 0.316818 1_3:+61635744 Msa0131060:CDS
AATAGAAGAATTATGCAATA+TGG 0.330857 1_3:-61637353 None:intergenic
CAAATAACAAAATATGGTTA+TGG 0.347669 1_3:-61637029 None:intergenic
TGATTTATGAGGTGAAAATA+TGG 0.352018 1_3:-61636610 None:intergenic
GCTGTAAACTGTGCTGTTAA+TGG 0.368748 1_3:+61635582 Msa0131060:CDS
TGGAGGCTCTCCTTGGTATA+AGG 0.368842 1_3:+61637173 Msa0131060:CDS
GTGTGGTTGGATGAGGAAAA+AGG 0.376961 1_3:-61634681 None:intergenic
TTCTTTCTTGGAGGTGTGAA+TGG 0.380455 1_3:-61636568 None:intergenic
CCGATAACTAGGCCTCCTTA+TGG 0.386117 1_3:+61636880 Msa0131060:CDS
TTTCTAATTCTTCTAGTACT+TGG 0.397904 1_3:+61635442 Msa0131060:CDS
ATGCAAATTCAATGGTGTTC+TGG 0.400255 1_3:+61635684 Msa0131060:CDS
GGAGAATCAGGCCAACGAAT+TGG 0.402668 1_3:-61635795 None:intergenic
CTTTGGAAACAATGGCTTAG+AGG 0.405587 1_3:+61635059 Msa0131060:CDS
AGGATTCTTCTAGGATTAGT+TGG 0.407748 1_3:+61635736 Msa0131060:CDS
TTCGTTGGCCTGATTCTCCT+TGG 0.409284 1_3:+61635799 Msa0131060:CDS
AGAGCAAAGAAAGGTGGAAT+TGG 0.414735 1_3:+61635402 Msa0131060:CDS
AGAAGGCGCCAAGGAGAATC+AGG 0.419546 1_3:-61635807 None:intergenic
CAACACAAGCCTGATCTTGT+TGG 0.424795 1_3:+61637201 Msa0131060:CDS
AAACCCTTTGATGAACTAAA+GGG 0.434794 1_3:+61637451 Msa0131060:three_prime_UTR
AAAATTCCTCTTCCACTTGT+GGG 0.435235 1_3:+61637516 Msa0131060:three_prime_UTR
CTTGTCTTGCTTGTTGAGTG+TGG 0.435888 1_3:+61636951 Msa0131060:CDS
CGTAGGCTTCATCAGTTTCA+GGG 0.436090 1_3:-61634978 None:intergenic
AAATGGTGGCAATGATTTGA+TGG 0.436140 1_3:+61635530 Msa0131060:CDS
TTAGCAATAATTGTCTGAAC+AGG 0.436415 1_3:-61635210 None:intergenic
AACCTTGGTTTCTTTCTTGG+AGG 0.437828 1_3:-61636577 None:intergenic
CCATAAGGAGGCCTAGTTAT+CGG 0.439826 1_3:-61636880 None:intergenic
CCATCAGAAGAATTCTCAAC+TGG 0.442393 1_3:-61637153 None:intergenic
AATGTCTTTGCAAAAGAAGC+TGG 0.444449 1_3:-61635093 None:intergenic
GAAACCTTAACACAAAATTC+AGG 0.450898 1_3:-61635645 None:intergenic
TTAAGAATAATTAGCTAGCT+AGG 0.455608 1_3:-61637293 None:intergenic
TAAACCCTTTGATGAACTAA+AGG 0.456993 1_3:+61637450 Msa0131060:three_prime_UTR
ATAGTCTCAGCACAGTATCT+AGG 0.458408 1_3:-61635156 None:intergenic
TGGTCCAAATTGAGCATGCC+TGG 0.458791 1_3:-61636761 None:intergenic
CTTCTGATGGAGGCTCTCCT+TGG 0.459576 1_3:+61637166 Msa0131060:CDS
AGATTAATGTTGGAGATTGA+TGG 0.460974 1_3:-61636658 None:intergenic
TCACCTTTGTTTGGTGGTGG+TGG 0.463200 1_3:+61635474 Msa0131060:CDS
ATGAATGAATTTCTATATCA+AGG 0.463564 1_3:+61637550 Msa0131060:three_prime_UTR
AGTGCTTAGATTCACAACTA+TGG 0.464164 1_3:+61634791 Msa0131060:CDS
AACCGTGTATCACCTTTGTT+TGG 0.470136 1_3:+61635465 Msa0131060:CDS
TTGTTATTGATACTCTTGTA+GGG 0.470816 1_3:-61636904 None:intergenic
AAATGTTACTGCATTGTGTA+TGG 0.470847 1_3:+61637230 Msa0131060:CDS
TTGAAGGGAATTAAATCAGA+TGG 0.471043 1_3:-61636793 None:intergenic
TTGTCTTGCTTGTTGAGTGT+GGG 0.476623 1_3:+61636952 Msa0131060:CDS
GTTTGTAGTTATGGATTCCA+TGG 0.477031 1_3:+61634753 Msa0131060:CDS
AAGTTTAGACATATCTATAG+AGG 0.484340 1_3:+61635255 Msa0131060:CDS
AATGGTGAGAGATTGAAGTT+TGG 0.485606 1_3:-61636550 None:intergenic
TACCCCTTTAGTTCATCAAA+GGG 0.485698 1_3:-61637454 None:intergenic
AATCACAAGAAGCTTGTTGC+TGG 0.491007 1_3:+61635324 Msa0131060:CDS
AATCTGCAATGCAAATTCAA+TGG 0.492272 1_3:+61635676 Msa0131060:CDS
ATACTCTTGTAGGGTCCATA+AGG 0.493770 1_3:-61636895 None:intergenic
GAAGAATCCTCAGTTTCAAA+AGG 0.493790 1_3:-61635723 None:intergenic
CATAATCCCACAAGTGGAAG+AGG 0.494235 1_3:-61637522 None:intergenic
TTGGTAACTTGTCATTGTCA+AGG 0.495636 1_3:+61636842 Msa0131060:CDS
CATCAAGCATACAGGGAGCC+AGG 0.498276 1_3:+61636743 Msa0131060:CDS
TTGTTGTAGTAGCTTGTTGA+AGG 0.499531 1_3:-61636809 None:intergenic
AATTCAGGAGTACTAGCTCT+TGG 0.500520 1_3:-61635630 None:intergenic
GATTGTTTCTTTGGAAACAA+TGG 0.500964 1_3:+61635051 Msa0131060:CDS
TATGGATCTTGAATAAACCT+TGG 0.506673 1_3:-61636592 None:intergenic
CGTGTATCACCTTTGTTTGG+TGG 0.513472 1_3:+61635468 Msa0131060:CDS
GAAAATTCCTCTTCCACTTG+TGG 0.514047 1_3:+61637515 Msa0131060:three_prime_UTR
ATAATATTGTTACCTGAAGA+AGG 0.514309 1_3:-61635824 None:intergenic
CAATGATTTGATGGGAAAAG+TGG 0.515215 1_3:+61635539 Msa0131060:CDS
CAACTATGGCACGCATGTGC+TGG 0.516615 1_3:+61634805 Msa0131060:CDS
TTTGGAAACAATGGCTTAGA+GGG 0.516902 1_3:+61635060 Msa0131060:CDS
TTACCCCTTTAGTTCATCAA+AGG 0.517930 1_3:-61637455 None:intergenic
GCATTGGAAGTTGATTTATG+AGG 0.518050 1_3:-61636621 None:intergenic
AAGAGTGGTTCTGTTTCTGA+AGG 0.520431 1_3:+61637093 Msa0131060:CDS
GTAATTGAGAAGATTAATGT+TGG 0.520472 1_3:-61636668 None:intergenic
ATCATCAACAACCGATAACT+AGG 0.523978 1_3:+61636869 Msa0131060:CDS
AGTTCAAATATTTCAAAGAG+TGG 0.525439 1_3:+61637078 Msa0131060:CDS
GGTGGTTCTGGTTTCTTGTG+TGG 0.526295 1_3:+61635492 Msa0131060:CDS
AGGATATGCATGGACAGTGT+TGG 0.527527 1_3:+61635232 Msa0131060:CDS
GTACTTGTTTAAGGCAATGA+GGG 0.529189 1_3:+61637493 Msa0131060:three_prime_UTR
GCAAAGAAAGGTGGAATTGG+TGG 0.533246 1_3:+61635405 Msa0131060:CDS
CCAGTTGAGAATTCTTCTGA+TGG 0.534523 1_3:+61637153 Msa0131060:CDS
GTATCACCTTTGTTTGGTGG+TGG 0.534706 1_3:+61635471 Msa0131060:CDS
AGAGAAAGTGAAGGTGTGGT+TGG 0.536093 1_3:-61634694 None:intergenic
GAAAATGGAGATCTTTGTGT+TGG 0.538803 1_3:+61635372 Msa0131060:CDS
TGTTCAGACAATTATTGCTA+AGG 0.546499 1_3:+61635212 Msa0131060:CDS
TTCATAGCAGAGATTCTACA+AGG 0.548674 1_3:-61634943 None:intergenic
TGTTGTTGATCCTTATACCA+AGG 0.550241 1_3:-61637183 None:intergenic
AGTGAAGGTGTGGTTGGATG+AGG 0.550919 1_3:-61634688 None:intergenic
GTTGTCAAAAGATGTCTCCT+TGG 0.557297 1_3:-61635282 None:intergenic
AAGAAAGGTGGAATTGGTGG+TGG 0.559157 1_3:+61635408 Msa0131060:CDS
AAGAGAAAAGAAAAGAAGAG+TGG 0.559701 1_3:-61634650 None:intergenic
GGTGATGATGATGATGGCAT+TGG 0.562514 1_3:-61636637 None:intergenic
GCAATAATTGTCTGAACAGG+TGG 0.564242 1_3:-61635207 None:intergenic
TTTAACATTGTGGAATGTGA+AGG 0.564248 1_3:+61637635 Msa0131060:three_prime_UTR
GAGACTAATTCAACTAACCA+AGG 0.569877 1_3:-61636520 None:intergenic
GCACGCATGTGCTGGTGCCA+TGG 0.574468 1_3:+61634813 Msa0131060:CDS
ATAAGGAGAGCAAAGAAAGG+TGG 0.575173 1_3:+61635396 Msa0131060:CDS
TGTACTTGTTTAAGGCAATG+AGG 0.575295 1_3:+61637492 Msa0131060:three_prime_UTR
TGTTGTAGTAGCTTGTTGAA+GGG 0.575894 1_3:-61636808 None:intergenic
AATGGTGGCAATGATTTGAT+GGG 0.576109 1_3:+61635531 Msa0131060:CDS
GGTATAAGGAGAGCAAAGAA+AGG 0.576664 1_3:+61635393 Msa0131060:CDS
TAGTTACATAATCCCACAAG+TGG 0.584945 1_3:-61637528 None:intergenic
ATTATTGCTAAGGATATGCA+TGG 0.587806 1_3:+61635222 Msa0131060:CDS
AAAATATGGTTATGGTTATG+GGG 0.588796 1_3:-61637021 None:intergenic
ATGTTCAAGATCCAATTCGT+TGG 0.592882 1_3:+61635784 Msa0131060:CDS
GTTGAGAATTCTTCTGATGG+AGG 0.594043 1_3:+61637156 Msa0131060:CDS
GAGATTCTACAAGGGATGAG+AGG 0.595750 1_3:-61634934 None:intergenic
TCTGCTGAATAATCCAACCT+AGG 0.603231 1_3:-61635183 None:intergenic
GCATGTTCTGCATGACCTTG+TGG 0.603875 1_3:-61634874 None:intergenic
AGACTAATTCAACTAACCAA+GGG 0.605653 1_3:-61636519 None:intergenic
ATGTGAAATGTGTAAACCCT+TGG 0.607377 1_3:+61636503 Msa0131060:CDS
AATCTCTGCTATGAAATACA+TGG 0.608031 1_3:+61634951 Msa0131060:CDS
TTAACATTGTGGAATGTGAA+GGG 0.613741 1_3:+61637636 Msa0131060:three_prime_UTR
AAGGGATGAGAGGTGGAACA+CGG 0.623921 1_3:-61634924 None:intergenic
GAACCACCACCACCAAACAA+AGG 0.625620 1_3:-61635477 None:intergenic
CACCAAACAAAGGTGATACA+CGG 0.628457 1_3:-61635467 None:intergenic
CTCTTGTAGGGTCCATAAGG+AGG 0.630872 1_3:-61636892 None:intergenic
ACACAATCTGATGCAAACAA+TGG 0.631167 1_3:+61635117 Msa0131060:CDS
ATTCTACAAGGGATGAGAGG+TGG 0.633006 1_3:-61634931 None:intergenic
ATTGATGGTGATGATGATGA+TGG 0.637177 1_3:-61636643 None:intergenic
CACCTCCAAGAAAGAAACCA+AGG 0.638580 1_3:+61636575 Msa0131060:CDS
AACCCTTTGATGAACTAAAG+GGG 0.646968 1_3:+61637452 Msa0131060:three_prime_UTR
AATGATTTGATGGGAAAAGT+GGG 0.649981 1_3:+61635540 Msa0131060:CDS
GTTACCTGAAGAAGGCGCCA+AGG 0.651106 1_3:-61635816 None:intergenic
GAGTGGTGGCATTTGAACCA+TGG 0.651177 1_3:-61634830 None:intergenic
AGGGATGAGAGGTGGAACAC+GGG 0.668889 1_3:-61634923 None:intergenic
AAAGTCTTCTACTTTCCACA+AGG 0.669890 1_3:+61634859 Msa0131060:CDS
TCATAGCAGAGATTCTACAA+GGG 0.672161 1_3:-61634942 None:intergenic
TCTGATGCAAACAATGGTGG+TGG 0.672693 1_3:+61635123 Msa0131060:CDS
GTGAAGAGAAAGTGAAGGTG+TGG 0.672989 1_3:-61634698 None:intergenic
CAATCTGATGCAAACAATGG+TGG 0.683286 1_3:+61635120 Msa0131060:CDS
TTGTTGTGAAGAGAAAGTGA+AGG 0.712448 1_3:-61634703 None:intergenic
ATATCTATAGAGGAACACCA+AGG 0.796890 1_3:+61635265 Msa0131060:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTCCAAATAACAAAATA+TGG - chr1_3:61637038-61637057 None:intergenic 15.0%
!!! ATTTTTTTTATCATTGTCTT+TGG + chr1_3:61637575-61637594 Msa0131060:three_prime_UTR 15.0%
!! TAAAAAGATGTTTGTAGTTA+TGG + chr1_3:61634744-61634763 Msa0131060:exon 20.0%
!! TAATTTAATTCTTGCTTAAC+AGG - chr1_3:61636173-61636192 None:intergenic 20.0%
!!! AAATCAGATGGATTATTATT+TGG - chr1_3:61636784-61636803 None:intergenic 20.0%
!! CAAATAACAAAATATGGTTA+TGG - chr1_3:61637032-61637051 None:intergenic 20.0%
!!! CATAACCATATTTTGTTATT+TGG + chr1_3:61637030-61637049 Msa0131060:CDS 20.0%
!! AAAAACAAATCATACAACAT+AGG - chr1_3:61637326-61637345 None:intergenic 20.0%
!! AATAGAAGAATTATGCAATA+TGG - chr1_3:61637356-61637375 None:intergenic 20.0%
!! TATTGCATAATTCTTCTATT+AGG + chr1_3:61637356-61637375 Msa0131060:three_prime_UTR 20.0%
!!! TTTTTTTTTGGAACAAAATG+AGG - chr1_3:61637402-61637421 None:intergenic 20.0%
!!! TATTAACTTGTACTTGTTTA+AGG + chr1_3:61637484-61637503 Msa0131060:three_prime_UTR 20.0%
!! ATGAATGAATTTCTATATCA+AGG + chr1_3:61637550-61637569 Msa0131060:three_prime_UTR 20.0%
!!! TTTAGCTTTTTTTAACATTG+TGG + chr1_3:61637625-61637644 Msa0131060:three_prime_UTR 20.0%
!! TTTGAAGAAGATTGTTTCTT+TGG + chr1_3:61635042-61635061 Msa0131060:CDS 25.0%
! AAGTTTAGACATATCTATAG+AGG + chr1_3:61635255-61635274 Msa0131060:CDS 25.0%
! TTTCTAATTCTTCTAGTACT+TGG + chr1_3:61635442-61635461 Msa0131060:CDS 25.0%
! ATAATATTGTTACCTGAAGA+AGG - chr1_3:61635827-61635846 None:intergenic 25.0%
! AACTTAATAACTTTCTCTCA+AGG - chr1_3:61636361-61636380 None:intergenic 25.0%
! TAAGTTTGTACCTACTTAAT+TGG + chr1_3:61636375-61636394 Msa0131060:intron 25.0%
!!! TTCACATTTTGAAGTAAATC+TGG - chr1_3:61636490-61636509 None:intergenic 25.0%
! TGATTTATGAGGTGAAAATA+TGG - chr1_3:61636613-61636632 None:intergenic 25.0%
! GTAATTGAGAAGATTAATGT+TGG - chr1_3:61636671-61636690 None:intergenic 25.0%
!! TTGTTATTGATACTCTTGTA+GGG - chr1_3:61636907-61636926 None:intergenic 25.0%
!! ATTGTTATTGATACTCTTGT+AGG - chr1_3:61636908-61636927 None:intergenic 25.0%
!! AAATTCTGGTCAAAGTTTTA+AGG + chr1_3:61636975-61636994 Msa0131060:CDS 25.0%
! AAAATATGGTTATGGTTATG+GGG - chr1_3:61637024-61637043 None:intergenic 25.0%
! CAAAATATGGTTATGGTTAT+GGG - chr1_3:61637025-61637044 None:intergenic 25.0%
! ACAAAATATGGTTATGGTTA+TGG - chr1_3:61637026-61637045 None:intergenic 25.0%
! AGTTCAAATATTTCAAAGAG+TGG + chr1_3:61637078-61637097 Msa0131060:CDS 25.0%
!!! AAATGATTTTCAAGACATCA+AGG + chr1_3:61637268-61637287 Msa0131060:three_prime_UTR 25.0%
! TTAAGAATAATTAGCTAGCT+AGG - chr1_3:61637296-61637315 None:intergenic 25.0%
!! TTTTAAGTAGAGATGAAGAC+AGG - chr1_3:61634608-61634627 None:intergenic 30.0%
AAGAGAAAAGAAAAGAAGAG+TGG - chr1_3:61634653-61634672 None:intergenic 30.0%
!!! CATCTTTTTAGAACGAAGTA+GGG - chr1_3:61634734-61634753 None:intergenic 30.0%
!!! ACATCTTTTTAGAACGAAGT+AGG - chr1_3:61634735-61634754 None:intergenic 30.0%
AATCTCTGCTATGAAATACA+TGG + chr1_3:61634951-61634970 Msa0131060:CDS 30.0%
! TAACTCGTTTTCTCTTAAAG+GGG - chr1_3:61635019-61635038 None:intergenic 30.0%
! CTAACTCGTTTTCTCTTAAA+GGG - chr1_3:61635020-61635039 None:intergenic 30.0%
! TCTAACTCGTTTTCTCTTAA+AGG - chr1_3:61635021-61635040 None:intergenic 30.0%
! GATTGTTTCTTTGGAAACAA+TGG + chr1_3:61635051-61635070 Msa0131060:CDS 30.0%
TTAGCAATAATTGTCTGAAC+AGG - chr1_3:61635213-61635232 None:intergenic 30.0%
TGTTCAGACAATTATTGCTA+AGG + chr1_3:61635212-61635231 Msa0131060:CDS 30.0%
ATTATTGCTAAGGATATGCA+TGG + chr1_3:61635222-61635241 Msa0131060:CDS 30.0%
GGAAATGAGAACAGAAAAAA+TGG + chr1_3:61635513-61635532 Msa0131060:CDS 30.0%
AATGAGAACAGAAAAAATGG+TGG + chr1_3:61635516-61635535 Msa0131060:CDS 30.0%
AATGATTTGATGGGAAAAGT+GGG + chr1_3:61635540-61635559 Msa0131060:CDS 30.0%
! TGTTAATGGAAGATCTTTTG+AGG + chr1_3:61635596-61635615 Msa0131060:CDS 30.0%
! TACTCCTGAATTTTGTGTTA+AGG + chr1_3:61635641-61635660 Msa0131060:CDS 30.0%
GAAACCTTAACACAAAATTC+AGG - chr1_3:61635648-61635667 None:intergenic 30.0%
AATCTGCAATGCAAATTCAA+TGG + chr1_3:61635676-61635695 Msa0131060:CDS 30.0%
!! TTCTAGGATTAGTTGGTTTA+TGG + chr1_3:61635743-61635762 Msa0131060:CDS 30.0%
!! TCTAGGATTAGTTGGTTTAT+GGG + chr1_3:61635744-61635763 Msa0131060:CDS 30.0%
AATTCAGTGCATCAATAAGA+TGG + chr1_3:61636006-61636025 Msa0131060:intron 30.0%
TTACTTCAAGCCAATTAAGT+AGG - chr1_3:61636388-61636407 None:intergenic 30.0%
! TTTATGATGTCATGTTTTCC+AGG + chr1_3:61636450-61636469 Msa0131060:intron 30.0%
AGACTAATTCAACTAACCAA+GGG - chr1_3:61636522-61636541 None:intergenic 30.0%
ATAAACCTTGGTTTCTTTCT+TGG - chr1_3:61636583-61636602 None:intergenic 30.0%
TATGGATCTTGAATAAACCT+TGG - chr1_3:61636595-61636614 None:intergenic 30.0%
AGATTAATGTTGGAGATTGA+TGG - chr1_3:61636661-61636680 None:intergenic 30.0%
TTGAAGGGAATTAAATCAGA+TGG - chr1_3:61636796-61636815 None:intergenic 30.0%
AAATGTTACTGCATTGTGTA+TGG + chr1_3:61637230-61637249 Msa0131060:CDS 30.0%
TAAACCCTTTGATGAACTAA+AGG + chr1_3:61637450-61637469 Msa0131060:three_prime_UTR 30.0%
AAACCCTTTGATGAACTAAA+GGG + chr1_3:61637451-61637470 Msa0131060:three_prime_UTR 30.0%
TTTAACATTGTGGAATGTGA+AGG + chr1_3:61637635-61637654 Msa0131060:three_prime_UTR 30.0%
TTAACATTGTGGAATGTGAA+GGG + chr1_3:61637636-61637655 Msa0131060:three_prime_UTR 30.0%
TTGTTGTGAAGAGAAAGTGA+AGG - chr1_3:61634706-61634725 None:intergenic 35.0%
GTTTGTAGTTATGGATTCCA+TGG + chr1_3:61634753-61634772 Msa0131060:CDS 35.0%
!! CTTTTCAGAGGTTTTTTCCA+TGG - chr1_3:61634773-61634792 None:intergenic 35.0%
! TGAATCTAAGCACTTTTCAG+AGG - chr1_3:61634785-61634804 None:intergenic 35.0%
AGTGCTTAGATTCACAACTA+TGG + chr1_3:61634791-61634810 Msa0131060:CDS 35.0%
! TAGAAGACTTTTGTGTTGAG+TGG - chr1_3:61634850-61634869 None:intergenic 35.0%
AAAGTCTTCTACTTTCCACA+AGG + chr1_3:61634859-61634878 Msa0131060:CDS 35.0%
TCATAGCAGAGATTCTACAA+GGG - chr1_3:61634945-61634964 None:intergenic 35.0%
TTCATAGCAGAGATTCTACA+AGG - chr1_3:61634946-61634965 None:intergenic 35.0%
!!! GGTTAGTTTCATTTTGACGT+AGG - chr1_3:61634998-61635017 None:intergenic 35.0%
TTTGGAAACAATGGCTTAGA+GGG + chr1_3:61635060-61635079 Msa0131060:CDS 35.0%
AATGTCTTTGCAAAAGAAGC+TGG - chr1_3:61635096-61635115 None:intergenic 35.0%
ACACAATCTGATGCAAACAA+TGG + chr1_3:61635117-61635136 Msa0131060:CDS 35.0%
!! TGAGACTATTTTTCCTAGGT+TGG + chr1_3:61635170-61635189 Msa0131060:CDS 35.0%
ATATCTATAGAGGAACACCA+AGG + chr1_3:61635265-61635284 Msa0131060:CDS 35.0%
!! GTGTTTTTGAGAGCAGAAAA+TGG + chr1_3:61635357-61635376 Msa0131060:CDS 35.0%
GAAAATGGAGATCTTTGTGT+TGG + chr1_3:61635372-61635391 Msa0131060:CDS 35.0%
! GAGATCTTTGTGTTGGTATA+AGG + chr1_3:61635379-61635398 Msa0131060:CDS 35.0%
AAATGGTGGCAATGATTTGA+TGG + chr1_3:61635530-61635549 Msa0131060:CDS 35.0%
AATGGTGGCAATGATTTGAT+GGG + chr1_3:61635531-61635550 Msa0131060:CDS 35.0%
CAATGATTTGATGGGAAAAG+TGG + chr1_3:61635539-61635558 Msa0131060:CDS 35.0%
ATGCAAATTCAATGGTGTTC+TGG + chr1_3:61635684-61635703 Msa0131060:CDS 35.0%
!!! CAAAATGCCTTTTGAAACTG+AGG + chr1_3:61635716-61635735 Msa0131060:CDS 35.0%
GAAGAATCCTCAGTTTCAAA+AGG - chr1_3:61635726-61635745 None:intergenic 35.0%
TTGAAACTGAGGATTCTTCT+AGG + chr1_3:61635727-61635746 Msa0131060:CDS 35.0%
!! AGGATTCTTCTAGGATTAGT+TGG + chr1_3:61635736-61635755 Msa0131060:CDS 35.0%
ATGTTCAAGATCCAATTCGT+TGG + chr1_3:61635784-61635803 Msa0131060:CDS 35.0%
CTATGGTAGATAGTACTAGT+AGG - chr1_3:61636312-61636331 None:intergenic 35.0%
ATTCTAGCAAGTAGCAACTA+TGG - chr1_3:61636329-61636348 None:intergenic 35.0%
! AGTTGCTACTTGCTAGAATT+TGG + chr1_3:61636330-61636349 Msa0131060:intron 35.0%
ATGTGAAATGTGTAAACCCT+TGG + chr1_3:61636503-61636522 Msa0131060:CDS 35.0%
GAGACTAATTCAACTAACCA+AGG - chr1_3:61636523-61636542 None:intergenic 35.0%
AATGGTGAGAGATTGAAGTT+TGG - chr1_3:61636553-61636572 None:intergenic 35.0%
! GCATTGGAAGTTGATTTATG+AGG - chr1_3:61636624-61636643 None:intergenic 35.0%
!! ATTGATGGTGATGATGATGA+TGG - chr1_3:61636646-61636665 None:intergenic 35.0%
! TTCTTTTGCATCAAGCATAC+AGG + chr1_3:61636735-61636754 Msa0131060:CDS 35.0%
! TCTTTTGCATCAAGCATACA+GGG + chr1_3:61636736-61636755 Msa0131060:CDS 35.0%
!! TGTTGTAGTAGCTTGTTGAA+GGG - chr1_3:61636811-61636830 None:intergenic 35.0%
!! TTGTTGTAGTAGCTTGTTGA+AGG - chr1_3:61636812-61636831 None:intergenic 35.0%
CTACAACAAGACATGTTTCT+TGG + chr1_3:61636823-61636842 Msa0131060:CDS 35.0%
! TTGGTAACTTGTCATTGTCA+AGG + chr1_3:61636842-61636861 Msa0131060:CDS 35.0%
ATCATCAACAACCGATAACT+AGG + chr1_3:61636869-61636888 Msa0131060:CDS 35.0%
TGTTGTTGATCCTTATACCA+AGG - chr1_3:61637186-61637205 None:intergenic 35.0%
! TTTTCTGTTCCAACAAGATC+AGG - chr1_3:61637213-61637232 None:intergenic 35.0%
AACCCTTTGATGAACTAAAG+GGG + chr1_3:61637452-61637471 Msa0131060:three_prime_UTR 35.0%
! TACCCCTTTAGTTCATCAAA+GGG - chr1_3:61637457-61637476 None:intergenic 35.0%
! TTACCCCTTTAGTTCATCAA+AGG - chr1_3:61637458-61637477 None:intergenic 35.0%
! TGTACTTGTTTAAGGCAATG+AGG + chr1_3:61637492-61637511 Msa0131060:three_prime_UTR 35.0%
! GTACTTGTTTAAGGCAATGA+GGG + chr1_3:61637493-61637512 Msa0131060:three_prime_UTR 35.0%
AAAATTCCTCTTCCACTTGT+GGG + chr1_3:61637516-61637535 Msa0131060:three_prime_UTR 35.0%
TAGTTACATAATCCCACAAG+TGG - chr1_3:61637531-61637550 None:intergenic 35.0%
! AAGACTTTTGTGTTGAGTGG+TGG - chr1_3:61634847-61634866 None:intergenic 40.0%
! CTTTGGAAACAATGGCTTAG+AGG + chr1_3:61635059-61635078 Msa0131060:CDS 40.0%
CAATCTGATGCAAACAATGG+TGG + chr1_3:61635120-61635139 Msa0131060:CDS 40.0%
ATAGTCTCAGCACAGTATCT+AGG - chr1_3:61635159-61635178 None:intergenic 40.0%
!! GTGCTGAGACTATTTTTCCT+AGG + chr1_3:61635166-61635185 Msa0131060:CDS 40.0%
TCTGCTGAATAATCCAACCT+AGG - chr1_3:61635186-61635205 None:intergenic 40.0%
GCAATAATTGTCTGAACAGG+TGG - chr1_3:61635210-61635229 None:intergenic 40.0%
GTTGTCAAAAGATGTCTCCT+TGG - chr1_3:61635285-61635304 None:intergenic 40.0%
! AGGAGACATCTTTTGACAAC+TGG + chr1_3:61635285-61635304 Msa0131060:CDS 40.0%
! GACATCTTTTGACAACTGGT+TGG + chr1_3:61635289-61635308 Msa0131060:CDS 40.0%
!! AATCACAAGAAGCTTGTTGC+TGG + chr1_3:61635324-61635343 Msa0131060:CDS 40.0%
GGTATAAGGAGAGCAAAGAA+AGG + chr1_3:61635393-61635412 Msa0131060:CDS 40.0%
ATAAGGAGAGCAAAGAAAGG+TGG + chr1_3:61635396-61635415 Msa0131060:CDS 40.0%
AGAGCAAAGAAAGGTGGAAT+TGG + chr1_3:61635402-61635421 Msa0131060:CDS 40.0%
AACCGTGTATCACCTTTGTT+TGG + chr1_3:61635465-61635484 Msa0131060:CDS 40.0%
CACCAAACAAAGGTGATACA+CGG - chr1_3:61635470-61635489 None:intergenic 40.0%
! GCTGTAAACTGTGCTGTTAA+TGG + chr1_3:61635582-61635601 Msa0131060:CDS 40.0%
!! AATTCAGGAGTACTAGCTCT+TGG - chr1_3:61635633-61635652 None:intergenic 40.0%
! TCATGTTTTCCAGGTAGTGT+GGG + chr1_3:61636459-61636478 Msa0131060:intron 40.0%
! TTCTTTCTTGGAGGTGTGAA+TGG - chr1_3:61636571-61636590 None:intergenic 40.0%
AACCTTGGTTTCTTTCTTGG+AGG - chr1_3:61636580-61636599 None:intergenic 40.0%
ATACTCTTGTAGGGTCCATA+AGG - chr1_3:61636898-61636917 None:intergenic 40.0%
! TTGTCTTGCTTGTTGAGTGT+GGG + chr1_3:61636952-61636971 Msa0131060:CDS 40.0%
TTGTTGAGTGTGGGAAATTC+TGG + chr1_3:61636961-61636980 Msa0131060:CDS 40.0%
! GGGTGCTTTTGCTTCAATAT+TGG - chr1_3:61637004-61637023 None:intergenic 40.0%
!! AAGAGTGGTTCTGTTTCTGA+AGG + chr1_3:61637093-61637112 Msa0131060:CDS 40.0%
CCATCAGAAGAATTCTCAAC+TGG - chr1_3:61637156-61637175 None:intergenic 40.0%
CCAGTTGAGAATTCTTCTGA+TGG + chr1_3:61637153-61637172 Msa0131060:CDS 40.0%
GTTGAGAATTCTTCTGATGG+AGG + chr1_3:61637156-61637175 Msa0131060:CDS 40.0%
GAAAATTCCTCTTCCACTTG+TGG + chr1_3:61637515-61637534 Msa0131060:three_prime_UTR 40.0%
GTGTGGTTGGATGAGGAAAA+AGG - chr1_3:61634684-61634703 None:intergenic 45.0%
! AGAGAAAGTGAAGGTGTGGT+TGG - chr1_3:61634697-61634716 None:intergenic 45.0%
GTGAAGAGAAAGTGAAGGTG+TGG - chr1_3:61634701-61634720 None:intergenic 45.0%
ATTCTACAAGGGATGAGAGG+TGG - chr1_3:61634934-61634953 None:intergenic 45.0%
GAGATTCTACAAGGGATGAG+AGG - chr1_3:61634937-61634956 None:intergenic 45.0%
CGTAGGCTTCATCAGTTTCA+GGG - chr1_3:61634981-61635000 None:intergenic 45.0%
ACGTAGGCTTCATCAGTTTC+AGG - chr1_3:61634982-61635001 None:intergenic 45.0%
TCTGATGCAAACAATGGTGG+TGG + chr1_3:61635123-61635142 Msa0131060:CDS 45.0%
AGGATATGCATGGACAGTGT+TGG + chr1_3:61635232-61635251 Msa0131060:CDS 45.0%
GCAAAGAAAGGTGGAATTGG+TGG + chr1_3:61635405-61635424 Msa0131060:CDS 45.0%
AAGAAAGGTGGAATTGGTGG+TGG + chr1_3:61635408-61635427 Msa0131060:CDS 45.0%
! CGTGTATCACCTTTGTTTGG+TGG + chr1_3:61635468-61635487 Msa0131060:CDS 45.0%
! GTATCACCTTTGTTTGGTGG+TGG + chr1_3:61635471-61635490 Msa0131060:CDS 45.0%
! GTCATGTTTTCCAGGTAGTG+TGG + chr1_3:61636458-61636477 Msa0131060:intron 45.0%
CACCTCCAAGAAAGAAACCA+AGG + chr1_3:61636575-61636594 Msa0131060:CDS 45.0%
!! GGTGATGATGATGATGGCAT+TGG - chr1_3:61636640-61636659 None:intergenic 45.0%
CCATAAGGAGGCCTAGTTAT+CGG - chr1_3:61636883-61636902 None:intergenic 45.0%
! CTTGTCTTGCTTGTTGAGTG+TGG + chr1_3:61636951-61636970 Msa0131060:CDS 45.0%
CAACACAAGCCTGATCTTGT+TGG + chr1_3:61637201-61637220 Msa0131060:CDS 45.0%
CATAATCCCACAAGTGGAAG+AGG - chr1_3:61637525-61637544 None:intergenic 45.0%
!!! ACATAATATTAATTTTTTTT+TGG - chr1_3:61637414-61637433 None:intergenic 5.0%
! AGTGAAGGTGTGGTTGGATG+AGG - chr1_3:61634691-61634710 None:intergenic 50.0%
GAGTGGTGGCATTTGAACCA+TGG - chr1_3:61634833-61634852 None:intergenic 50.0%
GCATGTTCTGCATGACCTTG+TGG - chr1_3:61634877-61634896 None:intergenic 50.0%
AAGGGATGAGAGGTGGAACA+CGG - chr1_3:61634927-61634946 None:intergenic 50.0%
!! TCACCTTTGTTTGGTGGTGG+TGG + chr1_3:61635474-61635493 Msa0131060:CDS 50.0%
GAACCACCACCACCAAACAA+AGG - chr1_3:61635480-61635499 None:intergenic 50.0%
!! TTGTTTGGTGGTGGTGGTTC+TGG + chr1_3:61635480-61635499 Msa0131060:CDS 50.0%
!! GGTGGTTCTGGTTTCTTGTG+TGG + chr1_3:61635492-61635511 Msa0131060:CDS 50.0%
GGAGAATCAGGCCAACGAAT+TGG - chr1_3:61635798-61635817 None:intergenic 50.0%
! TTCGTTGGCCTGATTCTCCT+TGG + chr1_3:61635799-61635818 Msa0131060:CDS 50.0%
TGGTCCAAATTGAGCATGCC+TGG - chr1_3:61636764-61636783 None:intergenic 50.0%
CCGATAACTAGGCCTCCTTA+TGG + chr1_3:61636880-61636899 Msa0131060:CDS 50.0%
CTCTTGTAGGGTCCATAAGG+AGG - chr1_3:61636895-61636914 None:intergenic 50.0%
TGGAGGCTCTCCTTGGTATA+AGG + chr1_3:61637173-61637192 Msa0131060:CDS 50.0%
CAACTATGGCACGCATGTGC+TGG + chr1_3:61634805-61634824 Msa0131060:CDS 55.0%
! GAGAGGTGGAACACGGGTTT+TGG - chr1_3:61634920-61634939 None:intergenic 55.0%
AGGGATGAGAGGTGGAACAC+GGG - chr1_3:61634926-61634945 None:intergenic 55.0%
!! AGAAGGCGCCAAGGAGAATC+AGG - chr1_3:61635810-61635829 None:intergenic 55.0%
TTCTCCTTGGCGCCTTCTTC+AGG + chr1_3:61635812-61635831 Msa0131060:CDS 55.0%
!! GTTACCTGAAGAAGGCGCCA+AGG - chr1_3:61635819-61635838 None:intergenic 55.0%
CTGGTTCATCCCACACTACC+TGG - chr1_3:61636471-61636490 None:intergenic 55.0%
CATCAAGCATACAGGGAGCC+AGG + chr1_3:61636743-61636762 Msa0131060:CDS 55.0%
GGAGCCAGGCATGCTCAATT+TGG + chr1_3:61636757-61636776 Msa0131060:CDS 55.0%
CTTCTGATGGAGGCTCTCCT+TGG + chr1_3:61637166-61637185 Msa0131060:CDS 55.0%
! GCACGCATGTGCTGGTGCCA+TGG + chr1_3:61634813-61634832 Msa0131060:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 61634567 61637668 61634567 ID=Msa0131060;Name=Msa0131060
chr1_3 mRNA 61634567 61637668 61634567 ID=Msa0131060-mRNA-1;Parent=Msa0131060;Name=Msa0131060-mRNA-1;_AED=0.00;_eAED=0.00;_QI=184|1|1|1|1|1|2|408|623
chr1_3 exon 61634567 61635833 61634567 ID=Msa0131060-mRNA-1:exon:20475;Parent=Msa0131060-mRNA-1
chr1_3 exon 61636472 61637668 61636472 ID=Msa0131060-mRNA-1:exon:20476;Parent=Msa0131060-mRNA-1
chr1_3 five_prime_UTR 61634567 61634750 61634567 ID=Msa0131060-mRNA-1:five_prime_utr;Parent=Msa0131060-mRNA-1
chr1_3 CDS 61634751 61635833 61634751 ID=Msa0131060-mRNA-1:cds;Parent=Msa0131060-mRNA-1
chr1_3 CDS 61636472 61637260 61636472 ID=Msa0131060-mRNA-1:cds;Parent=Msa0131060-mRNA-1
chr1_3 three_prime_UTR 61637261 61637668 61637261 ID=Msa0131060-mRNA-1:three_prime_utr;Parent=Msa0131060-mRNA-1
Gene Sequence

>Msa0131060

ATGTTTGTAGTTATGGATTCCATGGAAAAAACCTCTGAAAAGTGCTTAGATTCACAACTATGGCACGCATGTGCTGGTGCCATGGTTCAAATGCCACCACTCAACACAAAAGTCTTCTACTTTCCACAAGGTCATGCAGAACATGCTCATAACAAAGTAGATTTTTCCAAAACCCGTGTTCCACCTCTCATCCCTTGTAGAATCTCTGCTATGAAATACATGGCAGACCCTGAAACTGATGAAGCCTACGTCAAAATGAAACTAACCCCTTTAAGAGAAAACGAGTTAGATTTTGAAGAAGATTGTTTCTTTGGAAACAATGGCTTAGAGGGTCAAGAAAAGCCAGCTTCTTTTGCAAAGACATTAACACAATCTGATGCAAACAATGGTGGTGGTTTTTCTGTTCCTAGATACTGTGCTGAGACTATTTTTCCTAGGTTGGATTATTCAGCAGAACCACCTGTTCAGACAATTATTGCTAAGGATATGCATGGACAGTGTTGGAAGTTTAGACATATCTATAGAGGAACACCAAGGAGACATCTTTTGACAACTGGTTGGAGTAATTTTGTGAATCACAAGAAGCTTGTTGCTGGTGATTCAATTGTGTTTTTGAGAGCAGAAAATGGAGATCTTTGTGTTGGTATAAGGAGAGCAAAGAAAGGTGGAATTGGTGGTGGAACTGATCAGTTTTCTAATTCTTCTAGTACTTGGAACCGTGTATCACCTTTGTTTGGTGGTGGTGGTTCTGGTTTCTTGTGTGGAAATGAGAACAGAAAAAATGGTGGCAATGATTTGATGGGAAAAGTGGGAGCTGAATCTGTTGTTGAAGCTGTAAACTGTGCTGTTAATGGAAGATCTTTTGAGGTTGTGTATTATCCAAGAGCTAGTACTCCTGAATTTTGTGTTAAGGTTTCTTCTGTTAAATCTGCAATGCAAATTCAATGGTGTTCTGGTATGAGATTCAAAATGCCTTTTGAAACTGAGGATTCTTCTAGGATTAGTTGGTTTATGGGAACAATTTCTTCTGTTCATGTTCAAGATCCAATTCGTTGGCCTGATTCTCCTTGGCGCCTTCTTCAGGTAGTGTGGGATGAACCAGATTTACTTCAAAATGTGAAATGTGTAAACCCTTGGTTAGTTGAATTAGTCTCAAACATGCCAAACTTCAATCTCTCACCATTCACACCTCCAAGAAAGAAACCAAGGTTTATTCAAGATCCATATTTTCACCTCATAAATCAACTTCCAATGCCATCATCATCATCACCATCAATCTCCAACATTAATCTTCTCAATTACACAAAATCATCTCTCTGTAACATTCAAGACACTACTACTAATAGTTCTTTTGCATCAAGCATACAGGGAGCCAGGCATGCTCAATTTGGACCAAATAATAATCCATCTGATTTAATTCCCTTCAACAAGCTACTACAACAAGACATGTTTCTTGGTAACTTGTCATTGTCAAGGTTTAATCATCAACAACCGATAACTAGGCCTCCTTATGGACCCTACAAGAGTATCAATAACAATAACAACACTAAGAACAATGTTGACTTGTCTTGCTTGTTGAGTGTGGGAAATTCTGGTCAAAGTTTTAAGGAATCCAATATTGAAGCAAAAGCACCCCATAACCATAACCATATTTTGTTATTTGGAAAAATCATTCACACTGAACAAAACAGTTCAAATATTTCAAAGAGTGGTTCTGTTTCTGAAGGAACTTCTTTGAAAACATCAAATGCTTCTTCTTCTGATCCAGTTGAGAATTCTTCTGATGGAGGCTCTCCTTGGTATAAGGATCAACAACACAAGCCTGATCTTGTTGGAACAGAAAATGTTACTGCATTGTGTATGGCTTCATAA
Protein sequence

>Msa0131060

MFVVMDSMEKTSEKCLDSQLWHACAGAMVQMPPLNTKVFYFPQGHAEHAHNKVDFSKTRVPPLIPCRISAMKYMADPETDEAYVKMKLTPLRENELDFEEDCFFGNNGLEGQEKPASFAKTLTQSDANNGGGFSVPRYCAETIFPRLDYSAEPPVQTIIAKDMHGQCWKFRHIYRGTPRRHLLTTGWSNFVNHKKLVAGDSIVFLRAENGDLCVGIRRAKKGGIGGGTDQFSNSSSTWNRVSPLFGGGGSGFLCGNENRKNGGNDLMGKVGAESVVEAVNCAVNGRSFEVVYYPRASTPEFCVKVSSVKSAMQIQWCSGMRFKMPFETEDSSRISWFMGTISSVHVQDPIRWPDSPWRLLQVVWDEPDLLQNVKCVNPWLVELVSNMPNFNLSPFTPPRKKPRFIQDPYFHLINQLPMPSSSSPSISNINLLNYTKSSLCNIQDTTTNSSFASSIQGARHAQFGPNNNPSDLIPFNKLLQQDMFLGNLSLSRFNHQQPITRPPYGPYKSINNNNNTKNNVDLSCLLSVGNSGQSFKESNIEAKAPHNHNHILLFGKIIHTEQNSSNISKSGSVSEGTSLKTSNASSSDPVENSSDGGSPWYKDQQHKPDLVGTENVTALCMAS*