AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0132420

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0132420 MtrunA17_Chr1g0208771 29.276 304 177 10 30 320 16 294 4.37e-24 102
Msa0132420 MtrunA17_Chr7g0233541 26.756 299 192 7 26 316 1 280 4.01e-23 97.1
Msa0132420 MtrunA17_Chr3g0131891 24.051 316 206 9 12 320 19 307 1.92e-21 93.2
Msa0132420 MtrunA17_Chr1g0163221 25.688 327 173 11 12 315 11 290 4.53e-20 89.4
Msa0132420 MtrunA17_Chr1g0163071 26.710 307 165 9 41 319 16 290 8.07e-20 88.2
Msa0132420 MtrunA17_Chr7g0233521 26.014 296 181 8 26 316 1 263 8.62e-20 87.4
Msa0132420 MtrunA17_Chr7g0233531 27.609 297 175 9 26 316 1 263 1.56e-18 84.0
Msa0132420 MtrunA17_Chr7g0233561 25.478 314 199 10 15 316 22 312 1.94e-18 84.3
Msa0132420 MtrunA17_Chr4g0067431 25.532 329 188 12 12 315 15 311 1.30e-16 79.3
Msa0132420 MtrunA17_Chr3g0103181 26.071 280 168 9 38 315 37 279 2.00e-13 69.3
Msa0132420 MtrunA17_Chr1g0163251 26.573 286 176 9 34 317 28 281 2.29e-13 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0132420 AT3G18990.1 24.432 352 199 12 12 319 11 339 2.77e-17 81.6
Msa0132420 AT4G33280.1 27.645 293 171 15 43 319 55 322 7.48e-14 71.6

Find 42 sgRNAs with CRISPR-Local

Find 292 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTTTGAGTAGTAAATTT+TGG 0.102919 1_3:+63455905 Msa0132420:CDS
AACAGGAGAGTGCTATGTTT+TGG 0.285292 1_3:+63457843 Msa0132420:CDS
TGATAAAACCACATGGCTAT+TGG 0.295086 1_3:+63455963 Msa0132420:CDS
TTGTTGAGGCGAAGAGATTT+TGG 0.299523 1_3:+63457867 Msa0132420:CDS
AGCCTTCTTGCTGAGATATC+TGG 0.323467 1_3:+63456279 Msa0132420:CDS
TCAGGAAGCTGAAGACATAT+AGG 0.408366 1_3:-63455943 None:intergenic
AGAAGGCTGCGATAGCGTTT+AGG 0.433134 1_3:-63456264 None:intergenic
CAGGAAGCTGAAGACATATA+GGG 0.442300 1_3:-63455942 None:intergenic
GTTGTGACTGCGGCAAGCCT+TGG 0.444590 1_3:-63457539 None:intergenic
AGCGTTTAGGCTCCTCTTGT+TGG 0.449128 1_3:-63456251 None:intergenic
AAACCACATGGCTATTGGAT+TGG 0.454515 1_3:+63455968 Msa0132420:CDS
CTTAACAACACGTCAACAAC+AGG 0.469084 1_3:+63457826 Msa0132420:CDS
AAATATGCTGATGGGATATG+AGG 0.485987 1_3:-63456094 None:intergenic
TTTAGGCTCCTCTTGTTGGT+TGG 0.495993 1_3:-63456247 None:intergenic
TGAATGTAGCACTGGAGCAT+TGG 0.496562 1_3:+63457510 Msa0132420:CDS
TTAAAGCTTAACTCTTGCAA+TGG 0.504842 1_3:+63457899 Msa0132420:CDS
CATTTCTTTGAATGTGTGAT+GGG 0.511289 1_3:-63457585 None:intergenic
ACGGGCATTGTCATTTGAGA+TGG 0.514098 1_3:-63456309 None:intergenic
GGATAGGTTAAATATGCTGA+TGG 0.514655 1_3:-63456103 None:intergenic
GGAAAACAAGGAGGATAGCA+TGG 0.518866 1_3:-63456160 None:intergenic
TCATCAAATCGTCGTTTCGA+AGG 0.518996 1_3:-63455879 None:intergenic
GAAGCTATTGAATGTAGCAC+TGG 0.527718 1_3:+63457502 Msa0132420:CDS
ACACATTCAAAGAAATGTGC+TGG 0.535216 1_3:+63457591 Msa0132420:CDS
GGTTTCCCATGGGGAAAACA+AGG 0.540143 1_3:-63456172 None:intergenic
CTCATGTGTTATTAAACAAG+TGG 0.542298 1_3:+63456007 Msa0132420:CDS
ACATTTCTTTGAATGTGTGA+TGG 0.547251 1_3:-63457586 None:intergenic
AGATGATAATAAGAAACTGC+AGG 0.560494 1_3:-63456207 None:intergenic
GAAGAAAACCAACCAACAAG+AGG 0.567197 1_3:+63456239 Msa0132420:CDS
GATAGGTTAAATATGCTGAT+GGG 0.588873 1_3:-63456102 None:intergenic
TGTGTTATTAAACAAGTGGA+AGG 0.590126 1_3:+63456011 Msa0132420:CDS
GAAAACAAGGAGGATAGCAT+GGG 0.593037 1_3:-63456159 None:intergenic
ACGGACAATAAATTGAAACT+AGG 0.597678 1_3:+63457959 Msa0132420:CDS
GTCCAGATATCTCAGCAAGA+AGG 0.611666 1_3:-63456281 None:intergenic
TATCGTCGACTATGAAACAA+AGG 0.632297 1_3:+63457470 Msa0132420:CDS
TGGAGCATTGGAGAGAGCCA+AGG 0.647399 1_3:+63457522 Msa0132420:CDS
GTCGACTATGAAACAAAGGA+AGG 0.665590 1_3:+63457474 Msa0132420:CDS
CGTGTTGTTAAGAAGTTGCG+CGG 0.667200 1_3:-63457815 None:intergenic
TGAAAGATTTGTTGTGACTG+CGG 0.679005 1_3:-63457549 None:intergenic
TTTCCAATCCAATAGCCATG+TGG 0.687940 1_3:-63455971 None:intergenic
AGCTTCCTGATAAAACCACA+TGG 0.693997 1_3:+63455956 Msa0132420:CDS
TTCCCATGGGGAAAACAAGG+AGG 0.706970 1_3:-63456169 None:intergenic
AAAACAAGGAGGATAGCATG+GGG 0.772194 1_3:-63456158 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAAAACAACTTAATTTA+AGG - chr1_3:63456408-63456427 None:intergenic 10.0%
!!! TAAGTTGTTTTTAATTTTTA+CGG + chr1_3:63456413-63456432 Msa0132420:intron 10.0%
!!! ACTTAAATTATTAGTTTTAT+CGG + chr1_3:63456724-63456743 Msa0132420:intron 10.0%
!! ATTAAAAACAACTTAATTTA+AGG - chr1_3:63456408-63456427 None:intergenic 10.0%
!!! TAAGTTGTTTTTAATTTTTA+CGG + chr1_3:63456413-63456432 Msa0132420:intron 10.0%
!!! ACTTAAATTATTAGTTTTAT+CGG + chr1_3:63456724-63456743 Msa0132420:intron 10.0%
!! TCTAATGATTAAAAAATCAT+AGG + chr1_3:63456594-63456613 Msa0132420:intron 15.0%
!! CTAATGATTAAAAAATCATA+GGG + chr1_3:63456595-63456614 Msa0132420:intron 15.0%
!! TCTAATGATTAAAAAATCAT+AGG + chr1_3:63456594-63456613 Msa0132420:intron 15.0%
!! CTAATGATTAAAAAATCATA+GGG + chr1_3:63456595-63456614 Msa0132420:intron 15.0%
!!! ATCAATAATTTTGCAAAACT+TGG - chr1_3:63455851-63455870 None:intergenic 20.0%
!! TTAATTTAAGGAAACAAAAC+GGG - chr1_3:63456396-63456415 None:intergenic 20.0%
!! CTTAATTTAAGGAAACAAAA+CGG - chr1_3:63456397-63456416 None:intergenic 20.0%
!!! GCATCTCAAATTATTTTAAT+AGG + chr1_3:63457433-63457452 Msa0132420:intron 20.0%
!!! TCAATATTACAACTTGTTAT+TGG - chr1_3:63457686-63457705 None:intergenic 20.0%
!!! AAATATCTTTTAATGATGGT+TGG + chr1_3:63457924-63457943 Msa0132420:CDS 20.0%
!!! ATCAATAATTTTGCAAAACT+TGG - chr1_3:63455851-63455870 None:intergenic 20.0%
!! TTAATTTAAGGAAACAAAAC+GGG - chr1_3:63456396-63456415 None:intergenic 20.0%
!! CTTAATTTAAGGAAACAAAA+CGG - chr1_3:63456397-63456416 None:intergenic 20.0%
!!! GCATCTCAAATTATTTTAAT+AGG + chr1_3:63457433-63457452 Msa0132420:intron 20.0%
!!! TCAATATTACAACTTGTTAT+TGG - chr1_3:63457686-63457705 None:intergenic 20.0%
!!! AAATATCTTTTAATGATGGT+TGG + chr1_3:63457924-63457943 Msa0132420:CDS 20.0%
!!! AGCTTTGAGTAGTAAATTTT+GGG + chr1_3:63455906-63455925 Msa0132420:CDS 25.0%
! ATAAAAATCTGCATACTCAA+CGG - chr1_3:63456331-63456350 None:intergenic 25.0%
! TAATTTAAGGAAACAAAACG+GGG - chr1_3:63456395-63456414 None:intergenic 25.0%
! AAAATAGTAGCAATACAATC+CGG + chr1_3:63456448-63456467 Msa0132420:intron 25.0%
! TTAACTAAGACAGCTATATA+CGG - chr1_3:63456500-63456519 None:intergenic 25.0%
! TGATTAAAAAATCATAGGGT+AGG + chr1_3:63456599-63456618 Msa0132420:intron 25.0%
! GATTAAAAAATCATAGGGTA+GGG + chr1_3:63456600-63456619 Msa0132420:intron 25.0%
! GCTTAACAATGATATATTTG+AGG - chr1_3:63456809-63456828 None:intergenic 25.0%
!! ACTTTTATGCATAACAAAGT+TGG - chr1_3:63456972-63456991 None:intergenic 25.0%
!! AAGCTGGTATTTAAAATTGT+GGG - chr1_3:63457165-63457184 None:intergenic 25.0%
!! AAAGCTGGTATTTAAAATTG+TGG - chr1_3:63457166-63457185 None:intergenic 25.0%
! ATATCCACATGTAAAATATG+TGG + chr1_3:63457272-63457291 Msa0132420:intron 25.0%
! ATCAAAAACTACAACTTTGA+TGG + chr1_3:63457368-63457387 Msa0132420:intron 25.0%
! ATGATAAAAACTGTCAAAAC+AGG + chr1_3:63457787-63457806 Msa0132420:intron 25.0%
!! GGACAAATATCTTTTAATGA+TGG + chr1_3:63457920-63457939 Msa0132420:CDS 25.0%
! ACAACTTCAAAAAAAAGTCT+TGG - chr1_3:63458016-63458035 None:intergenic 25.0%
!!! AGCTTTGAGTAGTAAATTTT+GGG + chr1_3:63455906-63455925 Msa0132420:CDS 25.0%
! ATAAAAATCTGCATACTCAA+CGG - chr1_3:63456331-63456350 None:intergenic 25.0%
! TAATTTAAGGAAACAAAACG+GGG - chr1_3:63456395-63456414 None:intergenic 25.0%
! AAAATAGTAGCAATACAATC+CGG + chr1_3:63456448-63456467 Msa0132420:intron 25.0%
! TTAACTAAGACAGCTATATA+CGG - chr1_3:63456500-63456519 None:intergenic 25.0%
! TGATTAAAAAATCATAGGGT+AGG + chr1_3:63456599-63456618 Msa0132420:intron 25.0%
! GATTAAAAAATCATAGGGTA+GGG + chr1_3:63456600-63456619 Msa0132420:intron 25.0%
! GCTTAACAATGATATATTTG+AGG - chr1_3:63456809-63456828 None:intergenic 25.0%
!! ACTTTTATGCATAACAAAGT+TGG - chr1_3:63456972-63456991 None:intergenic 25.0%
!! AAGCTGGTATTTAAAATTGT+GGG - chr1_3:63457165-63457184 None:intergenic 25.0%
!! AAAGCTGGTATTTAAAATTG+TGG - chr1_3:63457166-63457185 None:intergenic 25.0%
! ATATCCACATGTAAAATATG+TGG + chr1_3:63457272-63457291 Msa0132420:intron 25.0%
! ATCAAAAACTACAACTTTGA+TGG + chr1_3:63457368-63457387 Msa0132420:intron 25.0%
! ATGATAAAAACTGTCAAAAC+AGG + chr1_3:63457787-63457806 Msa0132420:intron 25.0%
!! GGACAAATATCTTTTAATGA+TGG + chr1_3:63457920-63457939 Msa0132420:CDS 25.0%
! ACAACTTCAAAAAAAAGTCT+TGG - chr1_3:63458016-63458035 None:intergenic 25.0%
!!! CAGCTTTGAGTAGTAAATTT+TGG + chr1_3:63455905-63455924 Msa0132420:CDS 30.0%
! CTCATGTGTTATTAAACAAG+TGG + chr1_3:63456007-63456026 Msa0132420:CDS 30.0%
! TGTGTTATTAAACAAGTGGA+AGG + chr1_3:63456011-63456030 Msa0132420:CDS 30.0%
GATAGGTTAAATATGCTGAT+GGG - chr1_3:63456105-63456124 None:intergenic 30.0%
CAGTTTCATCAAAAATGGAT+AGG - chr1_3:63456122-63456141 None:intergenic 30.0%
!! CTATCCATTTTTGATGAAAC+TGG + chr1_3:63456120-63456139 Msa0132420:CDS 30.0%
AGATGATAATAAGAAACTGC+AGG - chr1_3:63456210-63456229 None:intergenic 30.0%
TAAAAATCTGCATACTCAAC+GGG - chr1_3:63456330-63456349 None:intergenic 30.0%
!!! ACCTCTTTTTTTAGGTTTTG+CGG - chr1_3:63456355-63456374 None:intergenic 30.0%
!!! ACTCATGTACCTCTTTTTTT+AGG - chr1_3:63456363-63456382 None:intergenic 30.0%
!!! TCAAGTTTTTGATTTGTTGC+CGG - chr1_3:63456470-63456489 None:intergenic 30.0%
TTAAAATTGTGGGATGAAGT+GGG - chr1_3:63457155-63457174 None:intergenic 30.0%
! TTTAAAATTGTGGGATGAAG+TGG - chr1_3:63457156-63457175 None:intergenic 30.0%
! AGAACCACATATTTTACATG+TGG - chr1_3:63457279-63457298 None:intergenic 30.0%
GTCATGTTGCTATAGAAATT+TGG - chr1_3:63457328-63457347 None:intergenic 30.0%
CATTTCTTTGAATGTGTGAT+GGG - chr1_3:63457588-63457607 None:intergenic 30.0%
ACATTTCTTTGAATGTGTGA+TGG - chr1_3:63457589-63457608 None:intergenic 30.0%
TTAAAGCTTAACTCTTGCAA+TGG + chr1_3:63457899-63457918 Msa0132420:CDS 30.0%
ACGGACAATAAATTGAAACT+AGG + chr1_3:63457959-63457978 Msa0132420:CDS 30.0%
!!! CAGCTTTGAGTAGTAAATTT+TGG + chr1_3:63455905-63455924 Msa0132420:CDS 30.0%
! CTCATGTGTTATTAAACAAG+TGG + chr1_3:63456007-63456026 Msa0132420:CDS 30.0%
! TGTGTTATTAAACAAGTGGA+AGG + chr1_3:63456011-63456030 Msa0132420:CDS 30.0%
GATAGGTTAAATATGCTGAT+GGG - chr1_3:63456105-63456124 None:intergenic 30.0%
CAGTTTCATCAAAAATGGAT+AGG - chr1_3:63456122-63456141 None:intergenic 30.0%
!! CTATCCATTTTTGATGAAAC+TGG + chr1_3:63456120-63456139 Msa0132420:CDS 30.0%
AGATGATAATAAGAAACTGC+AGG - chr1_3:63456210-63456229 None:intergenic 30.0%
TAAAAATCTGCATACTCAAC+GGG - chr1_3:63456330-63456349 None:intergenic 30.0%
!!! ACCTCTTTTTTTAGGTTTTG+CGG - chr1_3:63456355-63456374 None:intergenic 30.0%
!!! ACTCATGTACCTCTTTTTTT+AGG - chr1_3:63456363-63456382 None:intergenic 30.0%
!!! TCAAGTTTTTGATTTGTTGC+CGG - chr1_3:63456470-63456489 None:intergenic 30.0%
TTAAAATTGTGGGATGAAGT+GGG - chr1_3:63457155-63457174 None:intergenic 30.0%
! TTTAAAATTGTGGGATGAAG+TGG - chr1_3:63457156-63457175 None:intergenic 30.0%
! AGAACCACATATTTTACATG+TGG - chr1_3:63457279-63457298 None:intergenic 30.0%
GTCATGTTGCTATAGAAATT+TGG - chr1_3:63457328-63457347 None:intergenic 30.0%
CATTTCTTTGAATGTGTGAT+GGG - chr1_3:63457588-63457607 None:intergenic 30.0%
ACATTTCTTTGAATGTGTGA+TGG - chr1_3:63457589-63457608 None:intergenic 30.0%
TTAAAGCTTAACTCTTGCAA+TGG + chr1_3:63457899-63457918 Msa0132420:CDS 30.0%
ACGGACAATAAATTGAAACT+AGG + chr1_3:63457959-63457978 Msa0132420:CDS 30.0%
!!! AATAGCCATGTGGTTTTATC+AGG - chr1_3:63455964-63455983 None:intergenic 35.0%
TGATAAAACCACATGGCTAT+TGG + chr1_3:63455963-63455982 Msa0132420:CDS 35.0%
AAATATGCTGATGGGATATG+AGG - chr1_3:63456097-63456116 None:intergenic 35.0%
GGATAGGTTAAATATGCTGA+TGG - chr1_3:63456106-63456125 None:intergenic 35.0%
! CATTTTTGATGAAACTGGCA+CGG + chr1_3:63456125-63456144 Msa0132420:CDS 35.0%
!!! TTGTTGTTTTGGTTTCCCAT+GGG - chr1_3:63456185-63456204 None:intergenic 35.0%
!!! TTTGTTGTTTTGGTTTCCCA+TGG - chr1_3:63456186-63456205 None:intergenic 35.0%
!!! AACTGCAGGTTTTGTTGTTT+TGG - chr1_3:63456196-63456215 None:intergenic 35.0%
ACCGCAAAACCTAAAAAAAG+AGG + chr1_3:63456351-63456370 Msa0132420:CDS 35.0%
TAGCTGTCTTAGTTAATTCG+TGG + chr1_3:63456504-63456523 Msa0132420:intron 35.0%
! TAATTCGTGGACTACCATAT+TGG + chr1_3:63456517-63456536 Msa0132420:intron 35.0%
CTATAGAAGGCAAACCAATA+TGG - chr1_3:63456534-63456553 None:intergenic 35.0%
!!! TATTAGTTTTATCGGCGTGT+CGG + chr1_3:63456732-63456751 Msa0132420:intron 35.0%
!! TTCAAATGGATGGGTTAAGT+GGG - chr1_3:63456832-63456851 None:intergenic 35.0%
!! TTTCAAATGGATGGGTTAAG+TGG - chr1_3:63456833-63456852 None:intergenic 35.0%
TTGCAAGCTTTCAAATGGAT+GGG - chr1_3:63456841-63456860 None:intergenic 35.0%
ATGTGCATTAGAAGTCATGA+GGG - chr1_3:63456942-63456961 None:intergenic 35.0%
TATGTGCATTAGAAGTCATG+AGG - chr1_3:63456943-63456962 None:intergenic 35.0%
! TATCGTCGACTATGAAACAA+AGG + chr1_3:63457470-63457489 Msa0132420:CDS 35.0%
!! TGAAAGATTTGTTGTGACTG+CGG - chr1_3:63457552-63457571 None:intergenic 35.0%
ACACATTCAAAGAAATGTGC+TGG + chr1_3:63457591-63457610 Msa0132420:CDS 35.0%
! TGGTTGGAAGAATTTTGTCA+CGG + chr1_3:63457940-63457959 Msa0132420:CDS 35.0%
!!! AATAGCCATGTGGTTTTATC+AGG - chr1_3:63455964-63455983 None:intergenic 35.0%
TGATAAAACCACATGGCTAT+TGG + chr1_3:63455963-63455982 Msa0132420:CDS 35.0%
AAATATGCTGATGGGATATG+AGG - chr1_3:63456097-63456116 None:intergenic 35.0%
GGATAGGTTAAATATGCTGA+TGG - chr1_3:63456106-63456125 None:intergenic 35.0%
! CATTTTTGATGAAACTGGCA+CGG + chr1_3:63456125-63456144 Msa0132420:CDS 35.0%
!!! TTGTTGTTTTGGTTTCCCAT+GGG - chr1_3:63456185-63456204 None:intergenic 35.0%
!!! TTTGTTGTTTTGGTTTCCCA+TGG - chr1_3:63456186-63456205 None:intergenic 35.0%
!!! AACTGCAGGTTTTGTTGTTT+TGG - chr1_3:63456196-63456215 None:intergenic 35.0%
ACCGCAAAACCTAAAAAAAG+AGG + chr1_3:63456351-63456370 Msa0132420:CDS 35.0%
TAGCTGTCTTAGTTAATTCG+TGG + chr1_3:63456504-63456523 Msa0132420:intron 35.0%
! TAATTCGTGGACTACCATAT+TGG + chr1_3:63456517-63456536 Msa0132420:intron 35.0%
CTATAGAAGGCAAACCAATA+TGG - chr1_3:63456534-63456553 None:intergenic 35.0%
!!! TATTAGTTTTATCGGCGTGT+CGG + chr1_3:63456732-63456751 Msa0132420:intron 35.0%
!! TTCAAATGGATGGGTTAAGT+GGG - chr1_3:63456832-63456851 None:intergenic 35.0%
!! TTTCAAATGGATGGGTTAAG+TGG - chr1_3:63456833-63456852 None:intergenic 35.0%
TTGCAAGCTTTCAAATGGAT+GGG - chr1_3:63456841-63456860 None:intergenic 35.0%
ATGTGCATTAGAAGTCATGA+GGG - chr1_3:63456942-63456961 None:intergenic 35.0%
TATGTGCATTAGAAGTCATG+AGG - chr1_3:63456943-63456962 None:intergenic 35.0%
! TATCGTCGACTATGAAACAA+AGG + chr1_3:63457470-63457489 Msa0132420:CDS 35.0%
!! TGAAAGATTTGTTGTGACTG+CGG - chr1_3:63457552-63457571 None:intergenic 35.0%
ACACATTCAAAGAAATGTGC+TGG + chr1_3:63457591-63457610 Msa0132420:CDS 35.0%
! TGGTTGGAAGAATTTTGTCA+CGG + chr1_3:63457940-63457959 Msa0132420:CDS 35.0%
TCATCAAATCGTCGTTTCGA+AGG - chr1_3:63455882-63455901 None:intergenic 40.0%
CAGGAAGCTGAAGACATATA+GGG - chr1_3:63455945-63455964 None:intergenic 40.0%
TCAGGAAGCTGAAGACATAT+AGG - chr1_3:63455946-63455965 None:intergenic 40.0%
AGCTTCCTGATAAAACCACA+TGG + chr1_3:63455956-63455975 Msa0132420:CDS 40.0%
AAACCACATGGCTATTGGAT+TGG + chr1_3:63455968-63455987 Msa0132420:CDS 40.0%
TTTCCAATCCAATAGCCATG+TGG - chr1_3:63455974-63455993 None:intergenic 40.0%
CGTGCCAGTTTCATCAAAAA+TGG - chr1_3:63456127-63456146 None:intergenic 40.0%
AAAACAAGGAGGATAGCATG+GGG - chr1_3:63456161-63456180 None:intergenic 40.0%
GAAAACAAGGAGGATAGCAT+GGG - chr1_3:63456162-63456181 None:intergenic 40.0%
!!! TGTTGTTTTGGTTTCCCATG+GGG - chr1_3:63456184-63456203 None:intergenic 40.0%
GAAGAAAACCAACCAACAAG+AGG + chr1_3:63456239-63456258 Msa0132420:CDS 40.0%
! CGTTGGTTGAGTTCTATAGA+AGG - chr1_3:63456547-63456566 None:intergenic 40.0%
CCACTTATGTTCAAACTCGT+TGG - chr1_3:63456564-63456583 None:intergenic 40.0%
CCAACGAGTTTGAACATAAG+TGG + chr1_3:63456561-63456580 Msa0132420:intron 40.0%
! GCACGAACACTTTTCAGATT+AGG + chr1_3:63456633-63456652 Msa0132420:intron 40.0%
!! TGAGGTTGATGCAAAAGTAG+TGG - chr1_3:63456791-63456810 None:intergenic 40.0%
!! TCAAATGGATGGGTTAAGTG+GGG - chr1_3:63456831-63456850 None:intergenic 40.0%
GTTGCAAGCTTTCAAATGGA+TGG - chr1_3:63456842-63456861 None:intergenic 40.0%
GTCTGTTGCAAGCTTTCAAA+TGG - chr1_3:63456846-63456865 None:intergenic 40.0%
GTCATGAGGGGAATTTAACA+AGG - chr1_3:63456929-63456948 None:intergenic 40.0%
TGTGCATTAGAAGTCATGAG+GGG - chr1_3:63456941-63456960 None:intergenic 40.0%
CTCGACAAGTAATTGTTCGT+TGG - chr1_3:63457037-63457056 None:intergenic 40.0%
ACGAACAATTACTTGTCGAG+CGG + chr1_3:63457037-63457056 Msa0132420:intron 40.0%
GGCAGTGAGCAAAATTACAA+CGG - chr1_3:63457082-63457101 None:intergenic 40.0%
! TTGCTCACTGCCATTTATTG+AGG + chr1_3:63457090-63457109 Msa0132420:intron 40.0%
! TCACTGCCATTTATTGAGGA+AGG + chr1_3:63457094-63457113 Msa0132420:intron 40.0%
CTGCATCCTTCCTCAATAAA+TGG - chr1_3:63457103-63457122 None:intergenic 40.0%
TGTGGAGTTTATGAGAAAGC+TGG - chr1_3:63457181-63457200 None:intergenic 40.0%
GTCTCACTTGAGACATGTTT+GGG + chr1_3:63457222-63457241 Msa0132420:intron 40.0%
TCTCACTTGAGACATGTTTG+GGG + chr1_3:63457223-63457242 Msa0132420:intron 40.0%
GTCGACTATGAAACAAAGGA+AGG + chr1_3:63457474-63457493 Msa0132420:CDS 40.0%
GAAGCTATTGAATGTAGCAC+TGG + chr1_3:63457502-63457521 Msa0132420:CDS 40.0%
CTTAACAACACGTCAACAAC+AGG + chr1_3:63457826-63457845 Msa0132420:CDS 40.0%
!!! AACAGGAGAGTGCTATGTTT+TGG + chr1_3:63457843-63457862 Msa0132420:CDS 40.0%
!!! ACAGGAGAGTGCTATGTTTT+GGG + chr1_3:63457844-63457863 Msa0132420:CDS 40.0%
!!! TGCTATGTTTTGGGTTGTTG+AGG + chr1_3:63457853-63457872 Msa0132420:CDS 40.0%
! TTGTTGAGGCGAAGAGATTT+TGG + chr1_3:63457867-63457886 Msa0132420:CDS 40.0%
!! GTGTGTTTTTCAGCTAGTTG+AGG + chr1_3:63457988-63458007 Msa0132420:CDS 40.0%
TCATCAAATCGTCGTTTCGA+AGG - chr1_3:63455882-63455901 None:intergenic 40.0%
CAGGAAGCTGAAGACATATA+GGG - chr1_3:63455945-63455964 None:intergenic 40.0%
TCAGGAAGCTGAAGACATAT+AGG - chr1_3:63455946-63455965 None:intergenic 40.0%
AGCTTCCTGATAAAACCACA+TGG + chr1_3:63455956-63455975 Msa0132420:CDS 40.0%
AAACCACATGGCTATTGGAT+TGG + chr1_3:63455968-63455987 Msa0132420:CDS 40.0%
TTTCCAATCCAATAGCCATG+TGG - chr1_3:63455974-63455993 None:intergenic 40.0%
CGTGCCAGTTTCATCAAAAA+TGG - chr1_3:63456127-63456146 None:intergenic 40.0%
AAAACAAGGAGGATAGCATG+GGG - chr1_3:63456161-63456180 None:intergenic 40.0%
GAAAACAAGGAGGATAGCAT+GGG - chr1_3:63456162-63456181 None:intergenic 40.0%
!!! TGTTGTTTTGGTTTCCCATG+GGG - chr1_3:63456184-63456203 None:intergenic 40.0%
GAAGAAAACCAACCAACAAG+AGG + chr1_3:63456239-63456258 Msa0132420:CDS 40.0%
! CGTTGGTTGAGTTCTATAGA+AGG - chr1_3:63456547-63456566 None:intergenic 40.0%
CCACTTATGTTCAAACTCGT+TGG - chr1_3:63456564-63456583 None:intergenic 40.0%
CCAACGAGTTTGAACATAAG+TGG + chr1_3:63456561-63456580 Msa0132420:intron 40.0%
! GCACGAACACTTTTCAGATT+AGG + chr1_3:63456633-63456652 Msa0132420:intron 40.0%
!! TGAGGTTGATGCAAAAGTAG+TGG - chr1_3:63456791-63456810 None:intergenic 40.0%
!! TCAAATGGATGGGTTAAGTG+GGG - chr1_3:63456831-63456850 None:intergenic 40.0%
GTTGCAAGCTTTCAAATGGA+TGG - chr1_3:63456842-63456861 None:intergenic 40.0%
GTCTGTTGCAAGCTTTCAAA+TGG - chr1_3:63456846-63456865 None:intergenic 40.0%
GTCATGAGGGGAATTTAACA+AGG - chr1_3:63456929-63456948 None:intergenic 40.0%
TGTGCATTAGAAGTCATGAG+GGG - chr1_3:63456941-63456960 None:intergenic 40.0%
CTCGACAAGTAATTGTTCGT+TGG - chr1_3:63457037-63457056 None:intergenic 40.0%
ACGAACAATTACTTGTCGAG+CGG + chr1_3:63457037-63457056 Msa0132420:intron 40.0%
GGCAGTGAGCAAAATTACAA+CGG - chr1_3:63457082-63457101 None:intergenic 40.0%
! TTGCTCACTGCCATTTATTG+AGG + chr1_3:63457090-63457109 Msa0132420:intron 40.0%
! TCACTGCCATTTATTGAGGA+AGG + chr1_3:63457094-63457113 Msa0132420:intron 40.0%
CTGCATCCTTCCTCAATAAA+TGG - chr1_3:63457103-63457122 None:intergenic 40.0%
TGTGGAGTTTATGAGAAAGC+TGG - chr1_3:63457181-63457200 None:intergenic 40.0%
GTCTCACTTGAGACATGTTT+GGG + chr1_3:63457222-63457241 Msa0132420:intron 40.0%
TCTCACTTGAGACATGTTTG+GGG + chr1_3:63457223-63457242 Msa0132420:intron 40.0%
GTCGACTATGAAACAAAGGA+AGG + chr1_3:63457474-63457493 Msa0132420:CDS 40.0%
GAAGCTATTGAATGTAGCAC+TGG + chr1_3:63457502-63457521 Msa0132420:CDS 40.0%
CTTAACAACACGTCAACAAC+AGG + chr1_3:63457826-63457845 Msa0132420:CDS 40.0%
!!! AACAGGAGAGTGCTATGTTT+TGG + chr1_3:63457843-63457862 Msa0132420:CDS 40.0%
!!! ACAGGAGAGTGCTATGTTTT+GGG + chr1_3:63457844-63457863 Msa0132420:CDS 40.0%
!!! TGCTATGTTTTGGGTTGTTG+AGG + chr1_3:63457853-63457872 Msa0132420:CDS 40.0%
! TTGTTGAGGCGAAGAGATTT+TGG + chr1_3:63457867-63457886 Msa0132420:CDS 40.0%
!! GTGTGTTTTTCAGCTAGTTG+AGG + chr1_3:63457988-63458007 Msa0132420:CDS 40.0%
GGAAAACAAGGAGGATAGCA+TGG - chr1_3:63456163-63456182 None:intergenic 45.0%
!!! TATCCTCCTTGTTTTCCCCA+TGG + chr1_3:63456166-63456185 Msa0132420:CDS 45.0%
!!! ATCCTCCTTGTTTTCCCCAT+GGG + chr1_3:63456167-63456186 Msa0132420:CDS 45.0%
TTTAGGCTCCTCTTGTTGGT+TGG - chr1_3:63456250-63456269 None:intergenic 45.0%
AGCCTTCTTGCTGAGATATC+TGG + chr1_3:63456279-63456298 Msa0132420:CDS 45.0%
GTCCAGATATCTCAGCAAGA+AGG - chr1_3:63456284-63456303 None:intergenic 45.0%
! ACGGGCATTGTCATTTGAGA+TGG - chr1_3:63456312-63456331 None:intergenic 45.0%
CAGATTAGGTGTATCTCGTG+TGG + chr1_3:63456647-63456666 Msa0132420:intron 45.0%
AGTGGTTGTCTCCCTACTAA+CGG - chr1_3:63456773-63456792 None:intergenic 45.0%
TCCTCTCTTCAAGTCAAGGA+GGG - chr1_3:63456886-63456905 None:intergenic 45.0%
TTCCTCTCTTCAAGTCAAGG+AGG - chr1_3:63456887-63456906 None:intergenic 45.0%
! TCCTTGACTTGAAGAGAGGA+AGG + chr1_3:63456886-63456905 Msa0132420:intron 45.0%
TCCTTCCTCTCTTCAAGTCA+AGG - chr1_3:63456890-63456909 None:intergenic 45.0%
CGTTGGAAGACATCTCTCTT+TGG - chr1_3:63457020-63457039 None:intergenic 45.0%
CGTCTCACTTGAGACATGTT+TGG + chr1_3:63457221-63457240 Msa0132420:intron 45.0%
! TGAATGTAGCACTGGAGCAT+TGG + chr1_3:63457510-63457529 Msa0132420:CDS 45.0%
TCGGAGCTTAATTCTGCGAT+TGG + chr1_3:63457747-63457766 Msa0132420:intron 45.0%
! CGTGTTGTTAAGAAGTTGCG+CGG - chr1_3:63457818-63457837 None:intergenic 45.0%
GGAAAACAAGGAGGATAGCA+TGG - chr1_3:63456163-63456182 None:intergenic 45.0%
!!! TATCCTCCTTGTTTTCCCCA+TGG + chr1_3:63456166-63456185 Msa0132420:CDS 45.0%
!!! ATCCTCCTTGTTTTCCCCAT+GGG + chr1_3:63456167-63456186 Msa0132420:CDS 45.0%
TTTAGGCTCCTCTTGTTGGT+TGG - chr1_3:63456250-63456269 None:intergenic 45.0%
AGCCTTCTTGCTGAGATATC+TGG + chr1_3:63456279-63456298 Msa0132420:CDS 45.0%
GTCCAGATATCTCAGCAAGA+AGG - chr1_3:63456284-63456303 None:intergenic 45.0%
! ACGGGCATTGTCATTTGAGA+TGG - chr1_3:63456312-63456331 None:intergenic 45.0%
CAGATTAGGTGTATCTCGTG+TGG + chr1_3:63456647-63456666 Msa0132420:intron 45.0%
AGTGGTTGTCTCCCTACTAA+CGG - chr1_3:63456773-63456792 None:intergenic 45.0%
TCCTCTCTTCAAGTCAAGGA+GGG - chr1_3:63456886-63456905 None:intergenic 45.0%
TTCCTCTCTTCAAGTCAAGG+AGG - chr1_3:63456887-63456906 None:intergenic 45.0%
! TCCTTGACTTGAAGAGAGGA+AGG + chr1_3:63456886-63456905 Msa0132420:intron 45.0%
TCCTTCCTCTCTTCAAGTCA+AGG - chr1_3:63456890-63456909 None:intergenic 45.0%
CGTTGGAAGACATCTCTCTT+TGG - chr1_3:63457020-63457039 None:intergenic 45.0%
CGTCTCACTTGAGACATGTT+TGG + chr1_3:63457221-63457240 Msa0132420:intron 45.0%
! TGAATGTAGCACTGGAGCAT+TGG + chr1_3:63457510-63457529 Msa0132420:CDS 45.0%
TCGGAGCTTAATTCTGCGAT+TGG + chr1_3:63457747-63457766 Msa0132420:intron 45.0%
! CGTGTTGTTAAGAAGTTGCG+CGG - chr1_3:63457818-63457837 None:intergenic 45.0%
!! TTATAATATTGAAATATAAT+CGG + chr1_3:63457728-63457747 Msa0132420:intron 5.0%
!! TTATAATATTGAAATATAAT+CGG + chr1_3:63457728-63457747 Msa0132420:intron 5.0%
TTCCCATGGGGAAAACAAGG+AGG - chr1_3:63456172-63456191 None:intergenic 50.0%
GGTTTCCCATGGGGAAAACA+AGG - chr1_3:63456175-63456194 None:intergenic 50.0%
AGCGTTTAGGCTCCTCTTGT+TGG - chr1_3:63456254-63456273 None:intergenic 50.0%
AGAAGGCTGCGATAGCGTTT+AGG - chr1_3:63456267-63456286 None:intergenic 50.0%
!! TTTTATCGGCGTGTCGGTGT+CGG + chr1_3:63456738-63456757 Msa0132420:intron 50.0%
GGTGTCCATGTCCGTTAGTA+GGG + chr1_3:63456759-63456778 Msa0132420:intron 50.0%
! TGTCTCCCTACTAACGGACA+TGG - chr1_3:63456767-63456786 None:intergenic 50.0%
GAAAGCTTGCAACAGACCAG+AGG + chr1_3:63456849-63456868 Msa0132420:intron 50.0%
CTCTCTTCAAGTCAAGGAGG+GGG - chr1_3:63456884-63456903 None:intergenic 50.0%
CCTCTCTTCAAGTCAAGGAG+GGG - chr1_3:63456885-63456904 None:intergenic 50.0%
GCATGCGGTGTACAACATTG+TGG - chr1_3:63457199-63457218 None:intergenic 50.0%
CTCAAGTGAGACGTAGCATG+CGG - chr1_3:63457214-63457233 None:intergenic 50.0%
TTCCCATGGGGAAAACAAGG+AGG - chr1_3:63456172-63456191 None:intergenic 50.0%
GGTTTCCCATGGGGAAAACA+AGG - chr1_3:63456175-63456194 None:intergenic 50.0%
AGCGTTTAGGCTCCTCTTGT+TGG - chr1_3:63456254-63456273 None:intergenic 50.0%
AGAAGGCTGCGATAGCGTTT+AGG - chr1_3:63456267-63456286 None:intergenic 50.0%
!! TTTTATCGGCGTGTCGGTGT+CGG + chr1_3:63456738-63456757 Msa0132420:intron 50.0%
GGTGTCCATGTCCGTTAGTA+GGG + chr1_3:63456759-63456778 Msa0132420:intron 50.0%
! TGTCTCCCTACTAACGGACA+TGG - chr1_3:63456767-63456786 None:intergenic 50.0%
GAAAGCTTGCAACAGACCAG+AGG + chr1_3:63456849-63456868 Msa0132420:intron 50.0%
CTCTCTTCAAGTCAAGGAGG+GGG - chr1_3:63456884-63456903 None:intergenic 50.0%
CCTCTCTTCAAGTCAAGGAG+GGG - chr1_3:63456885-63456904 None:intergenic 50.0%
GCATGCGGTGTACAACATTG+TGG - chr1_3:63457199-63457218 None:intergenic 50.0%
CTCAAGTGAGACGTAGCATG+CGG - chr1_3:63457214-63457233 None:intergenic 50.0%
CGGTGTCCATGTCCGTTAGT+AGG + chr1_3:63456758-63456777 Msa0132420:intron 55.0%
TCAAGTCAAGGAGGGGGATG+CGG - chr1_3:63456878-63456897 None:intergenic 55.0%
! CCCCTCCTTGACTTGAAGAG+AGG + chr1_3:63456882-63456901 Msa0132420:intron 55.0%
GAGCGGCTGCACTAGTGTTT+GGG + chr1_3:63457054-63457073 Msa0132420:intron 55.0%
!! TGGAGCATTGGAGAGAGCCA+AGG + chr1_3:63457522-63457541 Msa0132420:CDS 55.0%
CGGTGTCCATGTCCGTTAGT+AGG + chr1_3:63456758-63456777 Msa0132420:intron 55.0%
TCAAGTCAAGGAGGGGGATG+CGG - chr1_3:63456878-63456897 None:intergenic 55.0%
! CCCCTCCTTGACTTGAAGAG+AGG + chr1_3:63456882-63456901 Msa0132420:intron 55.0%
GAGCGGCTGCACTAGTGTTT+GGG + chr1_3:63457054-63457073 Msa0132420:intron 55.0%
!! TGGAGCATTGGAGAGAGCCA+AGG + chr1_3:63457522-63457541 Msa0132420:CDS 55.0%
CGAGCGGCTGCACTAGTGTT+TGG + chr1_3:63457053-63457072 Msa0132420:intron 60.0%
GTTGTGACTGCGGCAAGCCT+TGG - chr1_3:63457542-63457561 None:intergenic 60.0%
CGAGCGGCTGCACTAGTGTT+TGG + chr1_3:63457053-63457072 Msa0132420:intron 60.0%
GTTGTGACTGCGGCAAGCCT+TGG - chr1_3:63457542-63457561 None:intergenic 60.0%
AGTCAAGGAGGGGGATGCGG+AGG - chr1_3:63456875-63456894 None:intergenic 65.0%
AGTCAAGGAGGGGGATGCGG+AGG - chr1_3:63456875-63456894 None:intergenic 65.0%
GAGGGGGATGCGGAGGCCTC+TGG - chr1_3:63456868-63456887 None:intergenic 75.0%
GAGGGGGATGCGGAGGCCTC+TGG - chr1_3:63456868-63456887 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 63455820 63458060 63455820 ID=Msa0132420;Name=Msa0132420
chr1_3 mRNA 63455820 63458060 63455820 ID=Msa0132420-mRNA-1;Parent=Msa0132420;Name=Msa0132420-mRNA-1;_AED=0.04;_eAED=0.12;_QI=0|0|0|1|1|1|3|0|320
chr1_3 exon 63455820 63456372 63455820 ID=Msa0132420-mRNA-1:exon:21247;Parent=Msa0132420-mRNA-1
chr1_3 exon 63457455 63457612 63457455 ID=Msa0132420-mRNA-1:exon:21248;Parent=Msa0132420-mRNA-1
chr1_3 exon 63457809 63458060 63457809 ID=Msa0132420-mRNA-1:exon:21249;Parent=Msa0132420-mRNA-1
chr1_3 CDS 63455820 63456372 63455820 ID=Msa0132420-mRNA-1:cds;Parent=Msa0132420-mRNA-1
chr1_3 CDS 63457455 63457612 63457455 ID=Msa0132420-mRNA-1:cds;Parent=Msa0132420-mRNA-1
chr1_3 CDS 63457809 63458060 63457809 ID=Msa0132420-mRNA-1:cds;Parent=Msa0132420-mRNA-1
Gene Sequence

>Msa0132420

ATGATATTTCAAGTTTCTGAAAGCGATACCAAGTTTTGCAAAATTATTGATGAAGACTGCCTTCGAAACGACGATTTGATGATTACAGCTTTGAGTAGTAAATTTTGGGATAATATTTCTAGCCCTATATGTCTTCAGCTTCCTGATAAAACCACATGGCTATTGGATTGGAAACAACATGATGATACTCATGTGTTATTAAACAAGTGGAAGGCATTTGCTGAAGCTTATTCTTTGAAGCATAATCAAGTTTTGCACTTCAACTACGTTGAAGCCTCATATCCCATCAGCATATTTAACCTATCCATTTTTGATGAAACTGGCACGGAAATATGTTACCCCATGCTATCCTCCTTGTTTTCCCCATGGGAAACCAAAACAACAAAACCTGCAGTTTCTTATTATCATCTCAATAGAATGAAGAAAACCAACCAACAAGAGGAGCCTAAACGCTATCGCAGCCTTCTTGCTGAGATATCTGGACAAACTCCATCTCAAATGACAATGCCCGTTGAGTATGCAGATTTTTATACCGCAAAACCTAAAAAAAGAGGTAGAAGAAGAGAAGTATCGTCGACTATGAAACAAAGGAAGGTTTTAGAAGCTATTGAATGTAGCACTGGAGCATTGGAGAGAGCCAAGGCTTGCCGCAGTCACAACAAATCTTTCATTCGTCAAATGCGCCCATCACACATTCAAAGAAATGTGCTGGTCATACCGCGCAACTTCTTAACAACACGTCAACAACAGGAGAGTGCTATGTTTTGGGTTGTTGAGGCGAAGAGATTTTGGCATGTCAAGTTAAAGCTTAACTCTTGCAATGGACAAATATCTTTTAATGATGGTTGGAAGAATTTTGTCACGGACAATAAATTGAAACTAGGTGATGTGTGTGTTTTTCAGCTAGTTGAGGCACCAAGACTTTTTTTTGAAGTTGTTATCTTTCGTGCAAGAGAACAATAA
Protein sequence

>Msa0132420

MIFQVSESDTKFCKIIDEDCLRNDDLMITALSSKFWDNISSPICLQLPDKTTWLLDWKQHDDTHVLLNKWKAFAEAYSLKHNQVLHFNYVEASYPISIFNLSIFDETGTEICYPMLSSLFSPWETKTTKPAVSYYHLNRMKKTNQQEEPKRYRSLLAEISGQTPSQMTMPVEYADFYTAKPKKRGRRREVSSTMKQRKVLEAIECSTGALERAKACRSHNKSFIRQMRPSHIQRNVLVIPRNFLTTRQQQESAMFWVVEAKRFWHVKLKLNSCNGQISFNDGWKNFVTDNKLKLGDVCVFQLVEAPRLFFEVVIFRAREQ*