AlfalfaGEDB | Msa0133030

M. sativa cultivar zhongmu-4 / Msa0133030

KEGG: K11644
GO: -
PFAM: PF02671.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0133030	G7KX87	60.449	445	139	14	1	421	1	432	7.83e-164	477

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TFs/TRs:

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Gene ID	Type	Classification
Msa0133030	TR	Others

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0133030	MtrunA17_Chr7g0252101	60.449	445	139	14	1	421	1	432	1.51e-167	477
Msa0133030	MtrunA17_Chr3g0093911	68.169	355	99	9	76	421	1	350	1.26e-156	446
Msa0133030	MtrunA17_Chr1g0207351	67.606	355	98	9	80	421	16	366	1.06e-152	436
Msa0133030	MtrunA17_Chr4g0075971	57.611	427	160	10	8	421	184	602	4.56e-149	436
Msa0133030	MtrunA17_Chr6g0488101	50.353	425	174	10	25	421	40	455	1.48e-130	384
Msa0133030	MtrunA17_Chr6g0488271	50.236	424	174	10	26	421	1	415	2.76e-130	381
Msa0133030	MtrunA17_Chr3g0077801	57.507	353	136	6	76	418	1	349	1.27e-129	377
Msa0133030	MtrunA17_Chr3g0077901	56.303	357	146	5	72	420	17	371	1.77e-125	367
Msa0133030	MtrunA17_Chr3g0077851	56.548	336	136	5	93	420	2	335	4.60e-118	347
Msa0133030	MtrunA17_Chr1g0209051	66.418	268	81	5	161	420	4	270	7.13e-116	339

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0133030	AT3G01320.1	46.667	75	31	1	18	92	75	140	5.22e-11	65.5
Msa0133030	AT3G01320.2	46.667	75	31	1	18	92	75	140	5.26e-11	65.5

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Find 100 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACACCAATCCAACATGA+AGG	0.599108	1_3:-64283578	None:intergenic
AAAAGTCCTAAAAGATATCA+AGG	0.441741	1_3:+64281059	Msa0133030:CDS
AAAATATCCGCTAGAAGATG+TGG	0.571308	1_3:-64282879	None:intergenic
AAAGCTTCCATTCAGAATCA+GGG	0.596310	1_3:+64280814	Msa0133030:five_prime_UTR
AACCACCAAGATAAATTCCT+CGG	0.558702	1_3:-64283038	None:intergenic
AAGGAACTTGCTCCTTGAGT+TGG	0.466623	1_3:-64283559	None:intergenic
AAGTACCCCTATGTTGGCCT+TGG	0.518606	1_3:-64283197	None:intergenic
AATATATGTTGTCACTACCA+AGG	0.650134	1_3:+64283180	Msa0133030:CDS
AATTTATACAAGAGGTGTTG+CGG	0.578757	1_3:+64281089	Msa0133030:CDS
ACACACCGGCAAATTGAAAA+AGG	0.404330	1_3:-64282679	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GACCAAAGAATTTATACAAG+AGG	+	chr1_3:64281081-64281100	Msa0133030:CDS	30.0%
CACCTCTTGTATAAATTCTT+TGG	-	chr1_3:64281086-64281105	None:intergenic	30.0%
AATAGAGTGGAATCATTGAA+TGG	-	chr1_3:64281582-64281601	None:intergenic	30.0%
TCTGAAGAAAAACAATAGAG+TGG	-	chr1_3:64281595-64281614	None:intergenic	30.0%
TTATTATAAGTCCCTTTGTG+GGG	-	chr1_3:64281924-64281943	None:intergenic	30.0%
CTTATTATAAGTCCCTTTGT+GGG	-	chr1_3:64281925-64281944	None:intergenic	30.0%
CACAAAGGGACTTATAATAA+GGG	+	chr1_3:64281924-64281943	Msa0133030:intron	30.0%
ATTAAGCTCAATGAAATCCT+AGG	-	chr1_3:64282044-64282063	None:intergenic	30.0%
TCTCATGAAGAAGAAAAAAG+AGG	+	chr1_3:64282389-64282408	Msa0133030:intron	30.0%
TTGTCATTGTTGTGATCTAA+AGG	+	chr1_3:64282572-64282591	Msa0133030:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	64280790	64283853	64280790	ID=Msa0133030;Name=Msa0133030
chr1_3	mRNA	64280790	64283853	64280790	ID=Msa0133030-mRNA-1;Parent=Msa0133030;Name=Msa0133030-mRNA-1;_AED=0.01;_eAED=0.01;_QI=102\|1\|1\|1\|0.5\|0.66\|3\|211\|421
chr1_3	exon	64280790	64280894	64280790	ID=Msa0133030-mRNA-1:exon:21546;Parent=Msa0133030-mRNA-1
chr1_3	exon	64280994	64281207	64280994	ID=Msa0133030-mRNA-1:exon:21547;Parent=Msa0133030-mRNA-1
chr1_3	exon	64282594	64283853	64282594	ID=Msa0133030-mRNA-1:exon:21548;Parent=Msa0133030-mRNA-1
chr1_3	five_prime_UTR	64280790	64280891	64280790	ID=Msa0133030-mRNA-1:five_prime_utr;Parent=Msa0133030-mRNA-1
chr1_3	CDS	64280892	64280894	64280892	ID=Msa0133030-mRNA-1:cds;Parent=Msa0133030-mRNA-1
chr1_3	CDS	64280994	64281207	64280994	ID=Msa0133030-mRNA-1:cds;Parent=Msa0133030-mRNA-1
chr1_3	CDS	64282594	64283642	64282594	ID=Msa0133030-mRNA-1:cds;Parent=Msa0133030-mRNA-1
chr1_3	three_prime_UTR	64283643	64283853	64283643	ID=Msa0133030-mRNA-1:three_prime_utr;Parent=Msa0133030-mRNA-1

Gene Sequence

>Msa0133030

ATGATGAAGATCCCCCAGAACTACCATGACGAGGTAGTACAAGCTGCGTTTAAACATAATGAGCTTTTAAAAGTCCTAAAAGATATCAAGGACCAAAGAATTTATACAAGAGGTGTTGCGGCAAGAGTCAAGGAGCTGCTTGAAGGGCATACTCATTTAACTTTGGGATTCAACACCTTCCTGCCAAAGGAACATCAAATCAAACTTGAGACCACTGGGAAAAAAGTGAAGAAGGTAGAAGAGAACCGTGAACTTCCTTTGGATGTGCTTGATTTCATTTCCCAAACACTAGATCTTGACGACCTTTTTCAATTTGCCGGTGTGTGCAAGAATTGGAAGGCTTTTCAGAAAATTTACTGGAAAAATTTCTTGGCATCCCAAGAACCATTAATTCTTCAAATTTCATATTATTGTACTAGATCAATTTCCTTGATTAGCCTACCCGACCAAAAAGTTTATTATTGCTTGAAGATAAAGAATTACTTCAAGGAATATATCTATGCCACATCTTCTAGCGGATATTTTATCATGGTGGGGAGAAATAATTCAGTTTTGCTAATCAATCCATTTACAAGAATAAAGAAGGTAATCAATAACTGCCCCTTTGAAGCCGAGTCTTATCATTATAGTAACCGTGCCTTGCTTGCTTTTGACAAACACTCCGAGGAATTTATCTTGGTGGTTTTCTGCTACTATCTTGGGAGTTTACATGTCTATCAATCTCGAAATTGTGGTTGGATTACTTATTCGACAATTGGAAATGAAAGGGGGAGGGTTGTTGACTTTGTGGTCTTGCATAATATAATATATGTTGTCACTACCAAGGCCAACATAGGGGTACTTAGCTTGACTTCTGCAAATATTAAATTTCTAAAATTGAAGAATACTCCCAATGAAAAATTTTCCCGTAGGTTAGTTAATTGTGATGAGCAACTTTTAGTGGTTGATTTAAGGTTCGGCGAAGTAAGGAATGTGTACAAGATAGACTTCTCAACCATGAATTATGTCAAACTAGAGACTTTGGGCGACATTGCATTATTTTATGCTTTGAACAGAAATTTTTATGCATTGAGCAACCCAAATACATGGGGATATGAGAGCAACTCTGTGTATGCACTGAGAAATCCTTTATGTAGTGTTTATTCAGGGGATGATAAAAAGTTGCAAAAATTGATTACGCTTCCAACTCAAGGAGCAAGTTCCTTCATGTTGGATTGGTGTTTTAGACATCTACAATATGAGGTAGATTATTCTCTAGTCGAGTGA

Protein sequence

>Msa0133030

MMKIPQNYHDEVVQAAFKHNELLKVLKDIKDQRIYTRGVAARVKELLEGHTHLTLGFNTFLPKEHQIKLETTGKKVKKVEENRELPLDVLDFISQTLDLDDLFQFAGVCKNWKAFQKIYWKNFLASQEPLILQISYYCTRSISLISLPDQKVYYCLKIKNYFKEYIYATSSSGYFIMVGRNNSVLLINPFTRIKKVINNCPFEAESYHYSNRALLAFDKHSEEFILVVFCYYLGSLHVYQSRNCGWITYSTIGNERGRVVDFVVLHNIIYVVTTKANIGVLSLTSANIKFLKLKNTPNEKFSRRLVNCDEQLLVVDLRFGEVRNVYKIDFSTMNYVKLETLGDIALFYALNRNFYALSNPNTWGYESNSVYALRNPLCSVYSGDDKKLQKLITLPTQGASSFMLDWCFRHLQYEVDYSLVE*