AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0133110

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0133110 MtrunA17_Chr1g0202461 95.513 312 13 1 1 311 77 388 0.0 521
Msa0133110 MtrunA17_Chr8g0348311 67.544 114 34 2 160 271 132 244 4.39e-46 159
Msa0133110 MtrunA17_Chr7g0248691 56.291 151 55 4 161 302 132 280 5.80e-46 158
Msa0133110 MtrunA17_Chr2g0293481 55.738 122 40 2 164 276 238 354 1.08e-37 139
Msa0133110 MtrunA17_Chr8g0387501 44.809 183 84 7 137 311 84 257 1.29e-35 129
Msa0133110 MtrunA17_Chr7g0270051 40.260 154 70 2 147 292 8 147 5.13e-32 120
Msa0133110 MtrunA17_Chr2g0289181 49.074 108 52 2 173 277 3 110 1.42e-31 117
Msa0133110 MtrunA17_Chr8g0379461 42.446 139 65 3 123 259 26 151 5.17e-28 108
Msa0133110 MtrunA17_Chr2g0284321 56.522 92 33 2 163 248 9 99 1.80e-27 106
Msa0133110 MtrunA17_Chr5g0401821 58.824 85 29 2 163 242 174 257 7.24e-26 103
Msa0133110 MtrunA17_Chr4g0053281 54.118 85 34 1 163 242 240 324 6.75e-25 102
Msa0133110 MtrunA17_Chr2g0319961 52.747 91 33 1 164 244 50 140 7.91e-25 100
Msa0133110 MtrunA17_Chr1g0184691 67.797 59 19 0 185 243 294 352 3.49e-23 98.2
Msa0133110 MtrunA17_Chr4g0071521 51.807 83 35 1 165 242 221 303 8.60e-23 96.3
Msa0133110 MtrunA17_Chr1g0147881 48.750 80 40 1 162 241 241 319 1.32e-21 93.6
Msa0133110 MtrunA17_Chr7g0228941 52.459 61 29 0 181 241 49 109 7.60e-20 83.6
Msa0133110 MtrunA17_Chr7g0218081 45.652 92 49 1 158 249 384 474 7.92e-20 89.7
Msa0133110 MtrunA17_Chr7g0218081 43.077 65 35 2 180 244 224 286 1.04e-11 65.5
Msa0133110 MtrunA17_Chr4g0074001 45.783 83 43 2 166 247 340 421 5.58e-19 87.0
Msa0133110 MtrunA17_Chr3g0139021 44.578 83 45 1 159 241 290 371 6.36e-19 86.7
Msa0133110 MtrunA17_Chr7g0249311 36.364 121 60 3 166 285 373 477 1.35e-17 83.2
Msa0133110 MtrunA17_Chr7g0249311 45.205 73 38 2 172 244 167 237 1.03e-14 74.7
Msa0133110 MtrunA17_Chr3g0099641 38.835 103 61 1 181 281 248 350 2.51e-17 82.0
Msa0133110 MtrunA17_Chr7g0238921 51.429 70 31 2 172 241 114 180 9.57e-17 79.3
Msa0133110 MtrunA17_Chr4g0042831 45.455 77 40 2 172 247 265 340 2.12e-16 79.0
Msa0133110 MtrunA17_Chr4g0042831 41.176 68 39 1 177 244 138 204 7.68e-12 65.5
Msa0133110 MtrunA17_Chr7g0270831 46.575 73 39 0 182 254 214 286 2.39e-16 79.0
Msa0133110 MtrunA17_Chr5g0418191 44.156 77 41 2 172 247 307 382 2.67e-16 79.0
Msa0133110 MtrunA17_Chr5g0418191 42.647 68 37 2 177 244 144 209 1.42e-11 64.7
Msa0133110 MtrunA17_Chr2g0302391 43.678 87 49 0 184 270 211 297 3.12e-16 79.0
Msa0133110 MtrunA17_Chr5g0447701 40.217 92 53 2 170 260 237 327 3.79e-16 78.2
Msa0133110 MtrunA17_Chr5g0447701 39.080 87 51 2 158 244 66 150 7.61e-12 65.5
Msa0133110 MtrunA17_Chr3g0079691 42.353 85 47 2 166 249 476 559 4.66e-16 78.6
Msa0133110 MtrunA17_Chr3g0079691 49.315 73 34 3 183 254 279 349 2.85e-13 70.5
Msa0133110 MtrunA17_Chr7g0263531 44.737 76 40 2 179 254 184 257 5.42e-16 78.2
Msa0133110 MtrunA17_Chr7g0263531 40.816 98 42 3 162 244 324 420 2.02e-15 76.3
Msa0133110 MtrunA17_Chr7g0249301 52.381 63 29 1 185 247 293 354 9.03e-16 77.0
Msa0133110 MtrunA17_Chr7g0249301 45.205 73 38 2 172 244 50 120 2.70e-15 75.9
Msa0133110 MtrunA17_Chr1g0194481 49.275 69 33 2 185 252 159 226 1.24e-15 76.3
Msa0133110 MtrunA17_Chr4g0004911 42.169 83 46 2 166 247 317 398 1.34e-15 77.0
Msa0133110 MtrunA17_Chr4g0004911 32.000 125 69 2 185 309 185 293 1.79e-13 70.9
Msa0133110 MtrunA17_Chr4g0051881 48.649 74 37 1 185 258 179 251 2.05e-15 75.9
Msa0133110 MtrunA17_Chr2g0322391 50.000 64 32 0 181 244 346 409 3.81e-15 75.9
Msa0133110 MtrunA17_Chr3g0106491 38.542 96 57 2 166 260 346 440 4.31e-15 75.5
Msa0133110 MtrunA17_Chr3g0106491 44.444 63 33 2 183 245 197 257 1.87e-11 64.7
Msa0133110 MtrunA17_Chr3g0101131 46.269 67 35 1 181 247 412 477 4.90e-15 75.5
Msa0133110 MtrunA17_Chr3g0101131 38.961 77 45 2 177 253 236 310 3.15e-11 63.9
Msa0133110 MtrunA17_Chr5g0445311 46.479 71 34 2 185 252 365 434 5.70e-15 75.5
Msa0133110 MtrunA17_Chr2g0288871 49.206 63 32 0 182 244 303 365 5.75e-15 75.5
Msa0133110 MtrunA17_Chr5g0443131 52.459 61 28 1 185 245 158 217 7.38e-15 73.9
Msa0133110 MtrunA17_Chr4g0025051 45.205 73 40 0 182 254 333 405 8.58e-15 74.7
Msa0133110 MtrunA17_Chr4g0001361 41.333 75 44 0 182 256 160 234 9.77e-15 73.6
Msa0133110 MtrunA17_Chr5g0432161 41.176 85 48 2 166 249 402 485 1.05e-14 74.7
Msa0133110 MtrunA17_Chr5g0432161 48.387 62 30 2 183 244 243 302 5.28e-12 66.2
Msa0133110 MtrunA17_Chr8g0383251 39.759 83 47 2 164 244 86 167 1.22e-14 70.9
Msa0133110 MtrunA17_Chr5g0418781 42.222 90 44 3 158 241 93 180 1.22e-14 72.8
Msa0133110 MtrunA17_Chr7g0243891 37.624 101 60 3 163 262 352 450 1.33e-14 74.3
Msa0133110 MtrunA17_Chr7g0243891 45.161 62 32 2 183 244 215 274 7.41e-12 65.9
Msa0133110 MtrunA17_Chr1g0150801 52.632 57 26 1 185 241 103 158 1.45e-14 70.5
Msa0133110 MtrunA17_Chr4g0051181 50.820 61 30 0 185 245 244 304 1.73e-14 73.6
Msa0133110 MtrunA17_Chr8g0387981 41.176 85 43 2 162 239 105 189 1.99e-14 73.2
Msa0133110 MtrunA17_Chr3g0126391 52.632 57 25 1 185 239 134 190 2.07e-14 72.8
Msa0133110 MtrunA17_Chr3g0136961 38.824 85 52 0 185 269 139 223 2.23e-14 72.4
Msa0133110 MtrunA17_Chr4g0075201 38.053 113 56 3 185 283 268 380 2.73e-14 73.2
Msa0133110 MtrunA17_Chr4g0025061 43.836 73 41 0 182 254 358 430 3.83e-14 72.8
Msa0133110 MtrunA17_Chr7g0270191 40.860 93 53 2 185 276 146 237 5.08e-14 71.2
Msa0133110 MtrunA17_Chr7g0216891 38.095 105 60 2 150 254 190 289 6.67e-14 72.0
Msa0133110 MtrunA17_Chr4g0068541 45.902 61 32 1 185 245 209 268 6.71e-14 71.6
Msa0133110 MtrunA17_Chr4g0060271 40.449 89 48 2 156 244 232 315 6.97e-14 72.0
Msa0133110 MtrunA17_Chr3g0129321 46.667 60 31 1 185 244 119 177 7.51e-14 69.3
Msa0133110 MtrunA17_Chr2g0294231 47.541 61 31 1 185 245 204 263 1.22e-13 70.9
Msa0133110 MtrunA17_Chr2g0333851 34.615 104 65 1 182 285 167 267 2.45e-13 69.3
Msa0133110 MtrunA17_Chr8g0335811 40.506 79 45 2 165 242 134 211 2.51e-13 68.2
Msa0133110 MtrunA17_Chr1g0150281 36.842 76 48 0 185 260 141 216 4.00e-13 68.6
Msa0133110 MtrunA17_Chr1g0189391 42.553 94 43 5 172 255 84 176 4.18e-13 68.9
Msa0133110 MtrunA17_Chr8g0347251 38.947 95 46 3 163 245 110 204 1.00e-12 67.8
Msa0133110 MtrunA17_Chr1g0150271 33.962 106 68 2 185 289 102 206 1.35e-12 66.6
Msa0133110 MtrunA17_Chr7g0275531 44.928 69 32 2 185 247 119 187 3.25e-12 66.2
Msa0133110 MtrunA17_Chr5g0447711 44.286 70 38 1 178 247 224 292 3.73e-12 65.9
Msa0133110 MtrunA17_Chr7g0224801 42.623 61 34 1 181 241 90 149 5.58e-12 63.2
Msa0133110 MtrunA17_Chr6g0485431 49.180 61 29 2 185 244 155 214 6.14e-12 65.5
Msa0133110 MtrunA17_Chr8g0383351 46.552 58 30 1 185 242 161 217 8.21e-12 63.9
Msa0133110 MtrunA17_Chr5g0428351 41.250 80 40 2 162 239 166 240 1.47e-11 64.3
Msa0133110 MtrunA17_Chr3g0129361 46.552 58 30 1 185 242 145 201 1.64e-11 62.8
Msa0133110 MtrunA17_Chr1g0188941 42.623 61 34 1 185 245 52 111 5.19e-11 58.9
Msa0133110 MtrunA17_Chr7g0248721 42.188 64 35 2 178 241 135 196 9.28e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0133110 AT5G52830.1 75.229 109 23 1 162 266 143 251 4.35e-53 177
Msa0133110 AT4G01250.1 40.755 265 85 8 1 257 1 201 5.15e-50 168
Msa0133110 AT4G23550.1 62.745 102 38 0 169 270 125 226 5.60e-39 139
Msa0133110 AT1G30650.1 46.067 178 67 5 156 305 177 353 2.29e-36 135
Msa0133110 AT2G34830.1 56.757 111 39 1 151 252 173 283 3.07e-36 135
Msa0133110 AT2G34830.2 56.757 111 39 1 151 252 182 292 3.32e-36 135
Msa0133110 AT5G45050.4 43.716 183 85 4 140 310 1003 1179 7.06e-33 129
Msa0133110 AT5G45050.2 43.716 183 85 4 140 310 1114 1290 7.55e-33 129
Msa0133110 AT5G45050.1 43.716 183 85 4 140 310 1142 1318 7.66e-33 129
Msa0133110 AT1G29280.1 65.278 72 20 1 184 255 74 140 9.36e-26 103
Msa0133110 AT3G58710.1 58.537 82 26 1 184 257 71 152 1.49e-25 103
Msa0133110 AT4G24240.1 58.824 85 29 2 163 242 256 339 1.62e-25 104
Msa0133110 AT3G58710.2 58.537 82 26 1 184 257 70 151 1.62e-25 103
Msa0133110 AT2G23320.1 52.381 84 36 1 163 242 215 298 5.75e-25 102
Msa0133110 AT5G28650.1 67.797 59 19 0 185 243 263 321 9.40e-24 99.8
Msa0133110 AT5G45260.1 49.505 101 47 3 161 258 1185 1284 1.27e-23 102
Msa0133110 AT3G04670.1 67.797 59 19 0 185 243 263 321 1.83e-23 99.0
Msa0133110 AT2G24570.1 51.220 82 38 1 162 241 219 300 3.10e-22 95.1
Msa0133110 AT4G31550.1 50.000 82 39 1 162 241 222 303 4.37e-22 94.7
Msa0133110 AT4G31550.2 48.780 82 40 1 162 241 221 302 1.44e-21 93.6
Msa0133110 AT2G30590.1 51.250 80 38 1 164 243 294 372 1.59e-20 91.3
Msa0133110 AT4G30935.1 36.364 132 79 3 168 295 314 444 2.81e-18 85.5
Msa0133110 AT2G04880.1 44.565 92 44 3 165 250 287 377 8.86e-18 84.0
Msa0133110 AT2G04880.2 44.565 92 44 3 165 250 263 353 9.73e-18 84.0
Msa0133110 AT4G26440.1 43.158 95 49 3 166 256 353 446 1.03e-16 80.9
Msa0133110 AT4G26440.1 34.286 140 74 5 118 256 127 249 4.51e-15 75.9
Msa0133110 AT3G01080.2 53.125 64 29 1 181 244 341 403 1.71e-16 80.1
Msa0133110 AT3G01080.2 42.308 78 43 2 180 257 201 276 8.49e-14 72.0
Msa0133110 AT3G01080.1 53.125 64 29 1 181 244 303 365 1.81e-16 79.7
Msa0133110 AT3G01080.1 42.308 78 43 2 180 257 163 238 9.34e-14 71.6
Msa0133110 AT5G56270.1 43.373 83 45 2 166 247 468 549 4.12e-16 79.3
Msa0133110 AT5G56270.1 48.333 60 29 2 185 244 274 331 3.90e-12 67.4
Msa0133110 AT4G04450.2 39.326 89 54 0 182 270 196 284 5.18e-16 78.6
Msa0133110 AT1G13960.1 41.176 85 48 2 161 244 385 468 6.28e-16 78.6
Msa0133110 AT1G13960.1 34.862 109 60 4 141 244 185 287 7.50e-13 69.3
Msa0133110 AT2G38470.1 39.091 110 62 3 172 277 349 457 8.75e-16 78.2
Msa0133110 AT2G38470.1 36.290 124 68 4 185 302 185 303 6.88e-13 69.3
Msa0133110 AT4G04450.1 39.326 89 54 0 182 270 290 378 9.37e-16 78.2
Msa0133110 AT1G13960.2 44.595 74 39 2 172 244 243 315 9.40e-16 77.4
Msa0133110 AT1G13960.2 34.862 109 60 4 141 244 32 134 2.07e-12 67.4
Msa0133110 AT1G69310.4 43.333 90 43 3 158 241 116 203 1.02e-15 76.3
Msa0133110 AT1G69310.2 43.333 90 43 3 158 241 116 203 1.02e-15 76.3
Msa0133110 AT1G69310.1 43.333 90 43 3 158 241 116 203 1.02e-15 76.3
Msa0133110 AT2G03340.1 43.038 79 43 2 172 249 402 479 1.38e-15 77.4
Msa0133110 AT2G03340.1 42.308 78 43 2 179 256 245 320 4.16e-13 70.1
Msa0133110 AT3G62340.1 35.115 131 80 3 163 289 95 224 1.95e-15 75.5
Msa0133110 AT5G07100.1 39.796 98 53 3 164 256 213 309 2.28e-15 75.9
Msa0133110 AT5G07100.1 41.176 68 39 1 177 244 110 176 1.54e-12 67.4
Msa0133110 AT4G22070.4 49.206 63 32 0 182 244 295 357 2.83e-15 76.6
Msa0133110 AT4G22070.3 49.206 63 32 0 182 244 295 357 2.83e-15 76.6
Msa0133110 AT4G22070.2 49.206 63 32 0 182 244 295 357 2.83e-15 76.6
Msa0133110 AT4G22070.1 49.206 63 32 0 182 244 295 357 2.83e-15 76.6
Msa0133110 AT5G07100.2 39.796 98 53 3 164 256 120 216 3.44e-15 73.6
Msa0133110 AT5G07100.2 41.176 68 39 1 177 244 17 83 9.94e-13 66.6
Msa0133110 AT5G07100.4 39.796 98 53 3 164 256 120 216 3.44e-15 73.6
Msa0133110 AT5G07100.4 41.176 68 39 1 177 244 17 83 9.94e-13 66.6
Msa0133110 AT5G07100.5 39.796 98 53 3 164 256 120 216 3.44e-15 73.6
Msa0133110 AT5G07100.5 41.176 68 39 1 177 244 17 83 9.94e-13 66.6
Msa0133110 AT5G07100.3 39.796 98 53 3 164 256 120 216 3.44e-15 73.6
Msa0133110 AT5G07100.3 41.176 68 39 1 177 244 17 83 9.94e-13 66.6
Msa0133110 AT2G21900.1 40.449 89 45 2 164 245 82 169 3.72e-15 73.2
Msa0133110 AT4G01720.1 53.333 60 28 0 185 244 240 299 3.84e-15 76.3
Msa0133110 AT1G68150.1 48.000 75 38 1 185 259 236 309 3.96e-15 75.5
Msa0133110 AT1G29860.1 43.590 78 40 2 185 262 137 210 4.05e-15 74.7
Msa0133110 AT2G40740.1 41.667 120 53 5 160 269 143 255 4.48e-15 74.7
Msa0133110 AT2G40740.3 41.667 120 53 5 160 269 152 264 4.78e-15 74.7
Msa0133110 AT1G62300.1 49.206 63 32 0 182 244 310 372 5.15e-15 75.9
Msa0133110 AT5G26170.1 51.724 58 27 1 185 242 114 170 6.02e-15 72.0
Msa0133110 AT2G37260.1 45.882 85 39 3 162 241 323 405 6.39e-15 75.1
Msa0133110 AT2G37260.1 41.935 93 52 2 178 270 159 249 1.38e-14 74.3
Msa0133110 AT5G46350.1 47.297 74 38 1 185 258 184 256 6.96e-15 74.3
Msa0133110 AT2G37260.3 45.882 85 39 3 162 241 243 325 8.15e-15 74.3
Msa0133110 AT2G37260.3 41.935 93 52 2 178 270 79 169 1.49e-14 73.6
Msa0133110 AT2G37260.2 45.882 85 39 3 162 241 241 323 8.43e-15 74.3
Msa0133110 AT2G37260.2 41.935 93 52 2 178 270 77 167 1.55e-14 73.6
Msa0133110 AT5G15130.1 45.714 70 38 0 185 254 228 297 1.02e-14 75.1
Msa0133110 AT5G15130.2 45.714 70 38 0 185 254 186 255 1.08e-14 74.7
Msa0133110 AT5G49520.1 50.769 65 30 2 180 244 218 280 1.22e-14 74.3
Msa0133110 AT4G18170.1 51.613 62 29 1 185 246 173 233 1.25e-14 73.6
Msa0133110 AT5G43290.1 39.560 91 54 1 151 241 81 170 1.34e-14 73.2
Msa0133110 AT3G01080.3 42.308 78 43 2 180 257 201 276 4.59e-14 72.0
Msa0133110 AT4G26640.7 43.478 69 38 1 181 249 338 405 5.87e-14 72.8
Msa0133110 AT4G26640.2 43.478 69 38 1 181 249 378 445 7.19e-14 72.4
Msa0133110 AT4G26640.1 43.478 69 38 1 181 249 306 373 7.51e-14 72.4
Msa0133110 AT4G23810.1 35.115 131 65 5 185 310 159 274 7.60e-14 71.6
Msa0133110 AT1G69810.1 47.887 71 36 1 185 254 204 274 7.98e-14 72.0
Msa0133110 AT4G26640.5 43.478 69 38 1 181 249 202 269 8.78e-14 71.6
Msa0133110 AT4G26640.6 43.478 69 38 1 181 249 202 269 8.78e-14 71.6
Msa0133110 AT4G26640.4 43.478 69 38 1 181 249 202 269 8.78e-14 71.6
Msa0133110 AT4G26640.3 43.478 69 38 1 181 249 202 269 8.78e-14 71.6
Msa0133110 AT1G80840.1 39.437 71 43 0 185 255 147 217 1.05e-13 70.9
Msa0133110 AT1G69810.2 47.887 71 36 1 185 254 146 216 1.07e-13 71.2
Msa0133110 AT2G30250.1 44.000 75 40 2 172 245 315 388 1.12e-13 71.2
Msa0133110 AT2G25000.2 46.667 60 32 0 185 244 104 163 2.20e-13 68.9
Msa0133110 AT2G25000.4 46.667 60 32 0 185 244 104 163 2.20e-13 68.9
Msa0133110 AT1G18860.1 50.000 60 30 0 185 244 240 299 2.66e-13 70.5
Msa0133110 AT2G44745.1 41.026 78 44 2 165 241 125 201 3.15e-13 68.2
Msa0133110 AT2G25000.3 46.667 60 32 0 185 244 126 185 3.46e-13 68.6
Msa0133110 AT4G31800.2 41.558 77 45 0 185 261 112 188 4.02e-13 68.6
Msa0133110 AT5G64810.1 49.123 57 28 1 185 241 111 166 4.47e-13 67.4
Msa0133110 AT2G40750.1 34.426 122 65 5 183 290 151 271 4.54e-13 69.3
Msa0133110 AT1G55600.1 37.500 80 48 2 166 244 288 366 4.55e-13 69.7
Msa0133110 AT2G25000.1 46.667 60 32 0 185 244 147 206 5.34e-13 68.6
Msa0133110 AT2G46130.1 49.206 63 30 2 179 241 26 86 6.04e-13 64.7
Msa0133110 AT4G31800.1 41.558 77 45 0 185 261 177 253 6.15e-13 68.6
Msa0133110 AT1G64000.1 45.161 62 33 1 180 241 110 170 8.05e-13 66.6
Msa0133110 AT5G41570.1 39.744 78 45 2 165 241 78 154 8.44e-13 66.2
Msa0133110 AT2G46400.1 33.333 117 72 3 129 239 45 161 8.67e-13 68.2
Msa0133110 AT2G47260.1 42.683 82 42 3 166 244 154 233 1.10e-12 68.2
Msa0133110 AT3G56400.1 41.026 78 39 3 164 239 105 177 1.50e-12 67.4
Msa0133110 AT3G01970.1 43.548 62 34 1 181 242 62 122 2.21e-12 64.3
Msa0133110 AT5G13080.1 42.623 61 34 1 181 241 64 123 2.45e-12 63.9
Msa0133110 AT4G11070.2 50.877 57 26 1 185 239 110 166 4.03e-12 65.9
Msa0133110 AT4G11070.1 50.877 57 26 1 185 239 142 198 4.17e-12 66.2
Msa0133110 AT5G24110.1 39.024 82 48 1 160 239 89 170 4.78e-12 65.9
Msa0133110 AT4G39410.1 42.667 75 42 1 185 259 224 297 9.20e-12 65.1

Find 95 sgRNAs with CRISPR-Local

Find 258 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAGTTTAAGAACAATATTT+AGG 0.223895 1_3:+64394446 Msa0133110:three_prime_UTR
TGGATGTGGAGAACCTTTAA+TGG 0.267330 1_3:-64393731 None:intergenic
TGCAATATGAGGAGGGGAAT+TGG 0.276980 1_3:-64393248 None:intergenic
TCTTGAAGCTCATTGGTTAT+AGG 0.277524 1_3:-64393157 None:intergenic
GTGTTTGAGGCTGGATGCTT+TGG 0.300180 1_3:-64393441 None:intergenic
CTTTCGCGCGGTACAACCTT+TGG 0.306785 1_3:-64393972 None:intergenic
ATAAATGTCACAAATGGTTT+TGG 0.307644 1_3:+64394385 Msa0133110:three_prime_UTR
TTGTGGGCATGGCGAAAATA+TGG 0.311492 1_3:+64393700 Msa0133110:CDS
TGTTTGAGGCTGGATGCTTT+GGG 0.315208 1_3:-64393440 None:intergenic
GTTATAGGTTGCACTACATT+AGG 0.317458 1_3:-64393142 None:intergenic
TCTTTAGCACCAAACTCTTT+TGG 0.322210 1_3:+64394175 Msa0133110:CDS
ACCTTCCCTTGGTCATGTTT+AGG 0.323922 1_3:-64394269 None:intergenic
GCAATATGAGGAGGGGAATT+GGG 0.331773 1_3:-64393247 None:intergenic
TCTTCTTCACCAAAAGAGTT+TGG 0.338560 1_3:-64394184 None:intergenic
TGTTCTTAAACTACTTCATT+TGG 0.344047 1_3:-64394437 None:intergenic
CTACCACTTCCTCTTCCATT+TGG 0.346152 1_3:+64393305 Msa0133110:CDS
TAGTTTAAGAACAATATTTA+GGG 0.348956 1_3:+64394447 Msa0133110:three_prime_UTR
AAACACCTAGATCAAGAAAA+AGG 0.350761 1_3:+64393458 Msa0133110:CDS
GGGTAAAAGATGTCAATAAT+TGG 0.354816 1_3:-64393186 None:intergenic
AGGAAGTGGTAGGGACAAAA+TGG 0.358832 1_3:-64393294 None:intergenic
TATAACAATGGCTGAGGATT+GGG 0.363678 1_3:+64392976 Msa0133110:exon
TCTGTTGTTGTTGTAATTGT+TGG 0.364018 1_3:-64393378 None:intergenic
TTGATCTAGGTGTTTGAGGC+TGG 0.368009 1_3:-64393450 None:intergenic
ATTTAAAACTTATATGCAAT+AGG 0.371007 1_3:+64394532 Msa0133110:three_prime_UTR
TGATTGGCAGCTTCGAACTA+TGG 0.377700 1_3:-64393008 None:intergenic
TTTGAAATTAGAAGAGGAAA+TGG 0.378100 1_3:-64393089 None:intergenic
TTTCTTGATCTAGGTGTTTG+AGG 0.387303 1_3:-64393454 None:intergenic
GTTGTTGTAATTGTTGGTTA+TGG 0.398557 1_3:-64393372 None:intergenic
GTTTAGATCTTGAAGCTCAT+TGG 0.407230 1_3:-64393164 None:intergenic
GTGGTCTTCAAACACTTTCA+CGG 0.411062 1_3:+64394297 Msa0133110:three_prime_UTR
GGATGTGGAGAACCTTTAAT+GGG 0.413222 1_3:-64393730 None:intergenic
TTCTGTTGTGGATGTATTGG+TGG 0.430239 1_3:-64393032 None:intergenic
TGGATGTATTGGTGGCTGAT+TGG 0.440667 1_3:-64393024 None:intergenic
ATGTTCACTGTGACATATAC+AGG 0.458166 1_3:+64394025 Msa0133110:CDS
CATGACCAAGGGAAGGTACT+TGG 0.460900 1_3:+64394275 Msa0133110:three_prime_UTR
TTATAACAATGGCTGAGGAT+TGG 0.466049 1_3:+64392975 Msa0133110:exon
CAAGAAGAAGTTGAGCTGTT+GGG 0.466337 1_3:-64394148 None:intergenic
TTGCACAAAATGAGGCCAAA+TGG 0.470578 1_3:-64393320 None:intergenic
CGGATGTGTCACCGGAGACT+TGG 0.472713 1_3:-64394104 None:intergenic
AGACTTGGTTCGTGTACTTC+CGG 0.473267 1_3:-64394089 None:intergenic
GCAAGAAGAAGTTGAGCTGT+TGG 0.473676 1_3:-64394149 None:intergenic
GAAGTTTCTTGCACAAAATG+AGG 0.474186 1_3:-64393328 None:intergenic
ACTATTTAAATATATGCCTC+TGG 0.485474 1_3:-64394499 None:intergenic
TGCATTTATAACAATGGCTG+AGG 0.489485 1_3:+64392970 Msa0133110:exon
CTCATTTGCATTTATAACAA+TGG 0.490614 1_3:+64392964 Msa0133110:exon
AGAGGTTATCTGCGGTTACA+TGG 0.495635 1_3:-64393669 None:intergenic
TAACCTCTCAACAGATTTGT+GGG 0.500103 1_3:+64393684 Msa0133110:CDS
AAAAGAGTTTGGTGCTAAAG+AGG 0.510944 1_3:-64394173 None:intergenic
TTCTACTTTGAAATTAGAAG+AGG 0.512786 1_3:-64393095 None:intergenic
TATTAGATAAATGTCACAAA+TGG 0.513172 1_3:+64394379 Msa0133110:three_prime_UTR
TTCCGGCTAGCGAGTTCCGA+TGG 0.517215 1_3:-64394072 None:intergenic
TCCCTTGGTCATGTTTAGGA+AGG 0.527622 1_3:-64394265 None:intergenic
AGGCCAAATGGAAGAGGAAG+TGG 0.528219 1_3:-64393308 None:intergenic
TTATATCTGTACCTTGGATG+TGG 0.528855 1_3:-64393745 None:intergenic
ATAACCTCTCAACAGATTTG+TGG 0.535451 1_3:+64393683 Msa0133110:CDS
CTAGTAGTAGTGGTGATGGT+GGG 0.535550 1_3:-64393208 None:intergenic
ACCCATCGGAACTCGCTAGC+CGG 0.537516 1_3:+64394070 Msa0133110:CDS
TCCGATGGGTAGGTCTTGCA+TGG 0.545684 1_3:-64394057 None:intergenic
CCTCATATTGCAAAATACAT+TGG 0.547832 1_3:+64393259 Msa0133110:CDS
TTAGAAGAGGAAATGGAAGA+TGG 0.555768 1_3:-64393082 None:intergenic
TACTTATGGGATTGTTCCAG+AGG 0.557829 1_3:+64394483 Msa0133110:three_prime_UTR
GACCACCAAGTACCTTCCCT+TGG 0.558663 1_3:-64394280 None:intergenic
AAAATGAGGCCAAATGGAAG+AGG 0.562724 1_3:-64393314 None:intergenic
GAGGTGGTCGGATGTGTCAC+CGG 0.563562 1_3:-64394112 None:intergenic
TAGTAGTAGTGGTGATGGTG+GGG 0.565045 1_3:-64393207 None:intergenic
GTTTGAGGCTGGATGCTTTG+GGG 0.566369 1_3:-64393439 None:intergenic
TGACTAGAAATTGAGGTGGT+CGG 0.577828 1_3:-64394124 None:intergenic
GAATATGTTGAAGCTAGAAG+TGG 0.578885 1_3:-64393350 None:intergenic
TCCGGCTAGCGAGTTCCGAT+GGG 0.583330 1_3:-64394071 None:intergenic
AGTAGTAGTGGTGATGGTGG+GGG 0.585398 1_3:-64393206 None:intergenic
TCTCAACAGATTTGTGGGCA+TGG 0.588913 1_3:+64393689 Msa0133110:CDS
GAAATTGAAACTGATGATGA+TGG 0.590055 1_3:+64394223 Msa0133110:CDS
GACCAAGGGAAGGTACTTGG+TGG 0.605286 1_3:+64394278 Msa0133110:three_prime_UTR
GTTGGGTTGACTAGAAATTG+AGG 0.607818 1_3:-64394131 None:intergenic
TTCCTTCCTAAACATGACCA+AGG 0.610457 1_3:+64394263 Msa0133110:exon
AAGTACACGAACCAAGTCTC+CGG 0.613947 1_3:+64394093 Msa0133110:CDS
TTAAAGGTTCTCCACATCCA+AGG 0.614226 1_3:+64393734 Msa0133110:CDS
AAATGGAAGAGGAAGTGGTA+GGG 0.618377 1_3:-64393303 None:intergenic
ACTATGACCACTAGTAGTAG+TGG 0.620104 1_3:-64393218 None:intergenic
TATAGATGTAGCAGTTCCAA+AGG 0.623205 1_3:+64393956 Msa0133110:CDS
CAAATGGAAGAGGAAGTGGT+AGG 0.623737 1_3:-64393304 None:intergenic
ACTAGTAGTAGTGGTGATGG+TGG 0.627374 1_3:-64393209 None:intergenic
CCTTTCAACTTGCTTTCGCG+CGG 0.627775 1_3:-64393984 None:intergenic
ATCTGTTGAGAGGTTATCTG+CGG 0.627787 1_3:-64393677 None:intergenic
GCTAGCGAGTTCCGATGGGT+AGG 0.628478 1_3:-64394067 None:intergenic
CCGCGCGAAAGCAAGTTGAA+AGG 0.629161 1_3:+64393984 Msa0133110:CDS
AGAAGAAGAGGACATCGACA+TGG 0.634163 1_3:+64394201 Msa0133110:CDS
ACCATCACCACTACTACTAG+TGG 0.637150 1_3:+64393211 Msa0133110:CDS
ACCACTAGTAGTAGTGGTGA+TGG 0.637486 1_3:-64393212 None:intergenic
ATGCCCACAAATCTGTTGAG+AGG 0.641173 1_3:-64393687 None:intergenic
TCCTTCCTAAACATGACCAA+GGG 0.654939 1_3:+64394264 Msa0133110:exon
AGATGGTATAGTATTTGTCG+TGG 0.665737 1_3:-64393065 None:intergenic
TCCTAAACATGACCAAGGGA+AGG 0.673965 1_3:+64394268 Msa0133110:exon
ACCATGCAAGACCTACCCAT+CGG 0.674071 1_3:+64394056 Msa0133110:CDS
GGGTTGACTAGAAATTGAGG+TGG 0.732424 1_3:-64394128 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACTCTAATAAAAATTAATT+TGG + chr1_3:64393766-64393785 Msa0133110:intron 10.0%
!!! AATCAATGTTTTAAAATTTT+TGG - chr1_3:64393836-64393855 None:intergenic 10.0%
!! AACTCTAATAAAAATTAATT+TGG + chr1_3:64393766-64393785 Msa0133110:intron 10.0%
!!! AATCAATGTTTTAAAATTTT+TGG - chr1_3:64393836-64393855 None:intergenic 10.0%
!!! AATATCACAACTATTTTTTT+GGG - chr1_3:64392930-64392949 None:intergenic 15.0%
!!! TAATATCACAACTATTTTTT+TGG - chr1_3:64392931-64392950 None:intergenic 15.0%
!!! TCTTCATTTTGTATATATAA+AGG + chr1_3:64394334-64394353 Msa0133110:three_prime_UTR 15.0%
!!! AAAGGTTATAAGAAATTTTT+TGG + chr1_3:64394352-64394371 Msa0133110:three_prime_UTR 15.0%
!! TAGTTTAAGAACAATATTTA+GGG + chr1_3:64394447-64394466 Msa0133110:three_prime_UTR 15.0%
!!! ATTTAAAACTTATATGCAAT+AGG + chr1_3:64394532-64394551 Msa0133110:three_prime_UTR 15.0%
!!! AATATCACAACTATTTTTTT+GGG - chr1_3:64392930-64392949 None:intergenic 15.0%
!!! TAATATCACAACTATTTTTT+TGG - chr1_3:64392931-64392950 None:intergenic 15.0%
!!! TCTTCATTTTGTATATATAA+AGG + chr1_3:64394334-64394353 Msa0133110:three_prime_UTR 15.0%
!!! AAAGGTTATAAGAAATTTTT+TGG + chr1_3:64394352-64394371 Msa0133110:three_prime_UTR 15.0%
!! TAGTTTAAGAACAATATTTA+GGG + chr1_3:64394447-64394466 Msa0133110:three_prime_UTR 15.0%
!!! ATTTAAAACTTATATGCAAT+AGG + chr1_3:64394532-64394551 Msa0133110:three_prime_UTR 15.0%
!!! ATATCACAACTATTTTTTTG+GGG - chr1_3:64392929-64392948 None:intergenic 20.0%
!!! TAATACAACAAAAAAGTGTA+AGG - chr1_3:64393925-64393944 None:intergenic 20.0%
!!! CACTTTTTTGTTGTATTATT+AGG + chr1_3:64393927-64393946 Msa0133110:intron 20.0%
!! TATTAGATAAATGTCACAAA+TGG + chr1_3:64394379-64394398 Msa0133110:three_prime_UTR 20.0%
!! GTAGTTTAAGAACAATATTT+AGG + chr1_3:64394446-64394465 Msa0133110:three_prime_UTR 20.0%
!!! ACATTTTCTAATGTACTTAT+GGG + chr1_3:64394470-64394489 Msa0133110:three_prime_UTR 20.0%
!!! ATATCACAACTATTTTTTTG+GGG - chr1_3:64392929-64392948 None:intergenic 20.0%
!!! TAATACAACAAAAAAGTGTA+AGG - chr1_3:64393925-64393944 None:intergenic 20.0%
!!! CACTTTTTTGTTGTATTATT+AGG + chr1_3:64393927-64393946 Msa0133110:intron 20.0%
!! TATTAGATAAATGTCACAAA+TGG + chr1_3:64394379-64394398 Msa0133110:three_prime_UTR 20.0%
!! GTAGTTTAAGAACAATATTT+AGG + chr1_3:64394446-64394465 Msa0133110:three_prime_UTR 20.0%
!!! ACATTTTCTAATGTACTTAT+GGG + chr1_3:64394470-64394489 Msa0133110:three_prime_UTR 20.0%
!!! TATCACAACTATTTTTTTGG+GGG - chr1_3:64392928-64392947 None:intergenic 25.0%
! CTCATTTGCATTTATAACAA+TGG + chr1_3:64392964-64392983 Msa0133110:exon 25.0%
!! TTTGAAATTAGAAGAGGAAA+TGG - chr1_3:64393092-64393111 None:intergenic 25.0%
!!! TTCTACTTTGAAATTAGAAG+AGG - chr1_3:64393098-64393117 None:intergenic 25.0%
! ATGTACTATTATTTATGCCA+TGG - chr1_3:64393568-64393587 None:intergenic 25.0%
! TTTCTGTTGAATAGGAAAAA+AGG - chr1_3:64393625-64393644 None:intergenic 25.0%
!! ATAAATGTCACAAATGGTTT+TGG + chr1_3:64394385-64394404 Msa0133110:three_prime_UTR 25.0%
! TGTTCTTAAACTACTTCATT+TGG - chr1_3:64394440-64394459 None:intergenic 25.0%
!! GACATTTTCTAATGTACTTA+TGG + chr1_3:64394469-64394488 Msa0133110:three_prime_UTR 25.0%
! ACTATTTAAATATATGCCTC+TGG - chr1_3:64394502-64394521 None:intergenic 25.0%
!!! TATCACAACTATTTTTTTGG+GGG - chr1_3:64392928-64392947 None:intergenic 25.0%
! CTCATTTGCATTTATAACAA+TGG + chr1_3:64392964-64392983 Msa0133110:exon 25.0%
!! TTTGAAATTAGAAGAGGAAA+TGG - chr1_3:64393092-64393111 None:intergenic 25.0%
!!! TTCTACTTTGAAATTAGAAG+AGG - chr1_3:64393098-64393117 None:intergenic 25.0%
! ATGTACTATTATTTATGCCA+TGG - chr1_3:64393568-64393587 None:intergenic 25.0%
! TTTCTGTTGAATAGGAAAAA+AGG - chr1_3:64393625-64393644 None:intergenic 25.0%
!! ATAAATGTCACAAATGGTTT+TGG + chr1_3:64394385-64394404 Msa0133110:three_prime_UTR 25.0%
! TGTTCTTAAACTACTTCATT+TGG - chr1_3:64394440-64394459 None:intergenic 25.0%
!! GACATTTTCTAATGTACTTA+TGG + chr1_3:64394469-64394488 Msa0133110:three_prime_UTR 25.0%
! ACTATTTAAATATATGCCTC+TGG - chr1_3:64394502-64394521 None:intergenic 25.0%
! ATTTTCTGTTGTGGATGTAT+TGG - chr1_3:64393038-64393057 None:intergenic 30.0%
! GGTAAATGAATTTTCTGTTG+TGG - chr1_3:64393047-64393066 None:intergenic 30.0%
GGGTAAAAGATGTCAATAAT+TGG - chr1_3:64393189-64393208 None:intergenic 30.0%
! TGTATTTTGCAATATGAGGA+GGG - chr1_3:64393258-64393277 None:intergenic 30.0%
! ATGTATTTTGCAATATGAGG+AGG - chr1_3:64393259-64393278 None:intergenic 30.0%
! CCAATGTATTTTGCAATATG+AGG - chr1_3:64393262-64393281 None:intergenic 30.0%
CCTCATATTGCAAAATACAT+TGG + chr1_3:64393259-64393278 Msa0133110:CDS 30.0%
! GTTGTTGTAATTGTTGGTTA+TGG - chr1_3:64393375-64393394 None:intergenic 30.0%
! TCTGTTGTTGTTGTAATTGT+TGG - chr1_3:64393381-64393400 None:intergenic 30.0%
AAACACCTAGATCAAGAAAA+AGG + chr1_3:64393458-64393477 Msa0133110:CDS 30.0%
! TACTACCTTTTTCTTGATCT+AGG - chr1_3:64393466-64393485 None:intergenic 30.0%
TACTATTATTTATGCCATGG+TGG - chr1_3:64393565-64393584 None:intergenic 30.0%
!! GTTGACTTTTTCTGTTGAAT+AGG - chr1_3:64393633-64393652 None:intergenic 30.0%
TTCAACAGAAAAAGTCAACA+AGG + chr1_3:64393634-64393653 Msa0133110:intron 30.0%
TCAACAGAAAAAGTCAACAA+GGG + chr1_3:64393635-64393654 Msa0133110:intron 30.0%
AAAAAGTCAACAAGGGAAAA+TGG + chr1_3:64393642-64393661 Msa0133110:CDS 30.0%
TATGGACAAAAACCCATTAA+AGG + chr1_3:64393718-64393737 Msa0133110:CDS 30.0%
TTAGAGTTATATCTGTACCT+TGG - chr1_3:64393754-64393773 None:intergenic 30.0%
TATTGAACCATCAATTTCTC+AGG + chr1_3:64393887-64393906 Msa0133110:intron 30.0%
AGTATTTCCTGAGAAATTGA+TGG - chr1_3:64393897-64393916 None:intergenic 30.0%
! GAAATTGAAACTGATGATGA+TGG + chr1_3:64394223-64394242 Msa0133110:CDS 30.0%
! ATTTTCTGTTGTGGATGTAT+TGG - chr1_3:64393038-64393057 None:intergenic 30.0%
! GGTAAATGAATTTTCTGTTG+TGG - chr1_3:64393047-64393066 None:intergenic 30.0%
GGGTAAAAGATGTCAATAAT+TGG - chr1_3:64393189-64393208 None:intergenic 30.0%
! TGTATTTTGCAATATGAGGA+GGG - chr1_3:64393258-64393277 None:intergenic 30.0%
! ATGTATTTTGCAATATGAGG+AGG - chr1_3:64393259-64393278 None:intergenic 30.0%
! CCAATGTATTTTGCAATATG+AGG - chr1_3:64393262-64393281 None:intergenic 30.0%
CCTCATATTGCAAAATACAT+TGG + chr1_3:64393259-64393278 Msa0133110:CDS 30.0%
! GTTGTTGTAATTGTTGGTTA+TGG - chr1_3:64393375-64393394 None:intergenic 30.0%
! TCTGTTGTTGTTGTAATTGT+TGG - chr1_3:64393381-64393400 None:intergenic 30.0%
AAACACCTAGATCAAGAAAA+AGG + chr1_3:64393458-64393477 Msa0133110:CDS 30.0%
! TACTACCTTTTTCTTGATCT+AGG - chr1_3:64393466-64393485 None:intergenic 30.0%
TACTATTATTTATGCCATGG+TGG - chr1_3:64393565-64393584 None:intergenic 30.0%
!! GTTGACTTTTTCTGTTGAAT+AGG - chr1_3:64393633-64393652 None:intergenic 30.0%
TTCAACAGAAAAAGTCAACA+AGG + chr1_3:64393634-64393653 Msa0133110:intron 30.0%
TCAACAGAAAAAGTCAACAA+GGG + chr1_3:64393635-64393654 Msa0133110:intron 30.0%
AAAAAGTCAACAAGGGAAAA+TGG + chr1_3:64393642-64393661 Msa0133110:CDS 30.0%
TATGGACAAAAACCCATTAA+AGG + chr1_3:64393718-64393737 Msa0133110:CDS 30.0%
TTAGAGTTATATCTGTACCT+TGG - chr1_3:64393754-64393773 None:intergenic 30.0%
TATTGAACCATCAATTTCTC+AGG + chr1_3:64393887-64393906 Msa0133110:intron 30.0%
AGTATTTCCTGAGAAATTGA+TGG - chr1_3:64393897-64393916 None:intergenic 30.0%
! GAAATTGAAACTGATGATGA+TGG + chr1_3:64394223-64394242 Msa0133110:CDS 30.0%
TGCATTTATAACAATGGCTG+AGG + chr1_3:64392970-64392989 Msa0133110:exon 35.0%
TTATAACAATGGCTGAGGAT+TGG + chr1_3:64392975-64392994 Msa0133110:exon 35.0%
TATAACAATGGCTGAGGATT+GGG + chr1_3:64392976-64392995 Msa0133110:exon 35.0%
AGATGGTATAGTATTTGTCG+TGG - chr1_3:64393068-64393087 None:intergenic 35.0%
TTAGAAGAGGAAATGGAAGA+TGG - chr1_3:64393085-64393104 None:intergenic 35.0%
GTTATAGGTTGCACTACATT+AGG - chr1_3:64393145-64393164 None:intergenic 35.0%
! TCTTGAAGCTCATTGGTTAT+AGG - chr1_3:64393160-64393179 None:intergenic 35.0%
GTTTAGATCTTGAAGCTCAT+TGG - chr1_3:64393167-64393186 None:intergenic 35.0%
! GTATTTTGCAATATGAGGAG+GGG - chr1_3:64393257-64393276 None:intergenic 35.0%
GAAGTTTCTTGCACAAAATG+AGG - chr1_3:64393331-64393350 None:intergenic 35.0%
GAATATGTTGAAGCTAGAAG+TGG - chr1_3:64393353-64393372 None:intergenic 35.0%
TTTCTTGATCTAGGTGTTTG+AGG - chr1_3:64393457-64393476 None:intergenic 35.0%
TTACATACATAAAGCCACCA+TGG + chr1_3:64393548-64393567 Msa0133110:intron 35.0%
ATAACCTCTCAACAGATTTG+TGG + chr1_3:64393683-64393702 Msa0133110:CDS 35.0%
TAACCTCTCAACAGATTTGT+GGG + chr1_3:64393684-64393703 Msa0133110:CDS 35.0%
TTATATCTGTACCTTGGATG+TGG - chr1_3:64393748-64393767 None:intergenic 35.0%
AGACGTAGTGTCAAATCTTT+CGG - chr1_3:64393810-64393829 None:intergenic 35.0%
TATAGATGTAGCAGTTCCAA+AGG + chr1_3:64393956-64393975 Msa0133110:CDS 35.0%
ATGTTCACTGTGACATATAC+AGG + chr1_3:64394025-64394044 Msa0133110:CDS 35.0%
!! AAAAGAGTTTGGTGCTAAAG+AGG - chr1_3:64394176-64394195 None:intergenic 35.0%
! TCTTTAGCACCAAACTCTTT+TGG + chr1_3:64394175-64394194 Msa0133110:CDS 35.0%
TCTTCTTCACCAAAAGAGTT+TGG - chr1_3:64394187-64394206 None:intergenic 35.0%
TGCATTTATAACAATGGCTG+AGG + chr1_3:64392970-64392989 Msa0133110:exon 35.0%
TTATAACAATGGCTGAGGAT+TGG + chr1_3:64392975-64392994 Msa0133110:exon 35.0%
TATAACAATGGCTGAGGATT+GGG + chr1_3:64392976-64392995 Msa0133110:exon 35.0%
AGATGGTATAGTATTTGTCG+TGG - chr1_3:64393068-64393087 None:intergenic 35.0%
TTAGAAGAGGAAATGGAAGA+TGG - chr1_3:64393085-64393104 None:intergenic 35.0%
GTTATAGGTTGCACTACATT+AGG - chr1_3:64393145-64393164 None:intergenic 35.0%
! TCTTGAAGCTCATTGGTTAT+AGG - chr1_3:64393160-64393179 None:intergenic 35.0%
GTTTAGATCTTGAAGCTCAT+TGG - chr1_3:64393167-64393186 None:intergenic 35.0%
! GTATTTTGCAATATGAGGAG+GGG - chr1_3:64393257-64393276 None:intergenic 35.0%
GAAGTTTCTTGCACAAAATG+AGG - chr1_3:64393331-64393350 None:intergenic 35.0%
GAATATGTTGAAGCTAGAAG+TGG - chr1_3:64393353-64393372 None:intergenic 35.0%
TTTCTTGATCTAGGTGTTTG+AGG - chr1_3:64393457-64393476 None:intergenic 35.0%
TTACATACATAAAGCCACCA+TGG + chr1_3:64393548-64393567 Msa0133110:intron 35.0%
ATAACCTCTCAACAGATTTG+TGG + chr1_3:64393683-64393702 Msa0133110:CDS 35.0%
TAACCTCTCAACAGATTTGT+GGG + chr1_3:64393684-64393703 Msa0133110:CDS 35.0%
TTATATCTGTACCTTGGATG+TGG - chr1_3:64393748-64393767 None:intergenic 35.0%
AGACGTAGTGTCAAATCTTT+CGG - chr1_3:64393810-64393829 None:intergenic 35.0%
TATAGATGTAGCAGTTCCAA+AGG + chr1_3:64393956-64393975 Msa0133110:CDS 35.0%
ATGTTCACTGTGACATATAC+AGG + chr1_3:64394025-64394044 Msa0133110:CDS 35.0%
!! AAAAGAGTTTGGTGCTAAAG+AGG - chr1_3:64394176-64394195 None:intergenic 35.0%
! TCTTTAGCACCAAACTCTTT+TGG + chr1_3:64394175-64394194 Msa0133110:CDS 35.0%
TCTTCTTCACCAAAAGAGTT+TGG - chr1_3:64394187-64394206 None:intergenic 35.0%
!!! CAACTATTTTTTTGGGGGTG+TGG - chr1_3:64392923-64392942 None:intergenic 40.0%
TTCTGTTGTGGATGTATTGG+TGG - chr1_3:64393035-64393054 None:intergenic 40.0%
ACTATGACCACTAGTAGTAG+TGG - chr1_3:64393221-64393240 None:intergenic 40.0%
AAATGGAAGAGGAAGTGGTA+GGG - chr1_3:64393306-64393325 None:intergenic 40.0%
AAAATGAGGCCAAATGGAAG+AGG - chr1_3:64393317-64393336 None:intergenic 40.0%
TTGCACAAAATGAGGCCAAA+TGG - chr1_3:64393323-64393342 None:intergenic 40.0%
ATCTGTTGAGAGGTTATCTG+CGG - chr1_3:64393680-64393699 None:intergenic 40.0%
GGATGTGGAGAACCTTTAAT+GGG - chr1_3:64393733-64393752 None:intergenic 40.0%
TGGATGTGGAGAACCTTTAA+TGG - chr1_3:64393734-64393753 None:intergenic 40.0%
TTAAAGGTTCTCCACATCCA+AGG + chr1_3:64393734-64393753 Msa0133110:CDS 40.0%
TGACTAGAAATTGAGGTGGT+CGG - chr1_3:64394127-64394146 None:intergenic 40.0%
!! GTTGGGTTGACTAGAAATTG+AGG - chr1_3:64394134-64394153 None:intergenic 40.0%
CAAGAAGAAGTTGAGCTGTT+GGG - chr1_3:64394151-64394170 None:intergenic 40.0%
! CTCTTTTGGTGAAGAAGAAG+AGG + chr1_3:64394189-64394208 Msa0133110:CDS 40.0%
TTCCTTCCTAAACATGACCA+AGG + chr1_3:64394263-64394282 Msa0133110:exon 40.0%
TCCTTCCTAAACATGACCAA+GGG + chr1_3:64394264-64394283 Msa0133110:exon 40.0%
GTGGTCTTCAAACACTTTCA+CGG + chr1_3:64394297-64394316 Msa0133110:three_prime_UTR 40.0%
TACTTATGGGATTGTTCCAG+AGG + chr1_3:64394483-64394502 Msa0133110:three_prime_UTR 40.0%
!!! CAACTATTTTTTTGGGGGTG+TGG - chr1_3:64392923-64392942 None:intergenic 40.0%
TTCTGTTGTGGATGTATTGG+TGG - chr1_3:64393035-64393054 None:intergenic 40.0%
ACTATGACCACTAGTAGTAG+TGG - chr1_3:64393221-64393240 None:intergenic 40.0%
AAATGGAAGAGGAAGTGGTA+GGG - chr1_3:64393306-64393325 None:intergenic 40.0%
AAAATGAGGCCAAATGGAAG+AGG - chr1_3:64393317-64393336 None:intergenic 40.0%
TTGCACAAAATGAGGCCAAA+TGG - chr1_3:64393323-64393342 None:intergenic 40.0%
ATCTGTTGAGAGGTTATCTG+CGG - chr1_3:64393680-64393699 None:intergenic 40.0%
GGATGTGGAGAACCTTTAAT+GGG - chr1_3:64393733-64393752 None:intergenic 40.0%
TGGATGTGGAGAACCTTTAA+TGG - chr1_3:64393734-64393753 None:intergenic 40.0%
TTAAAGGTTCTCCACATCCA+AGG + chr1_3:64393734-64393753 Msa0133110:CDS 40.0%
TGACTAGAAATTGAGGTGGT+CGG - chr1_3:64394127-64394146 None:intergenic 40.0%
!! GTTGGGTTGACTAGAAATTG+AGG - chr1_3:64394134-64394153 None:intergenic 40.0%
CAAGAAGAAGTTGAGCTGTT+GGG - chr1_3:64394151-64394170 None:intergenic 40.0%
! CTCTTTTGGTGAAGAAGAAG+AGG + chr1_3:64394189-64394208 Msa0133110:CDS 40.0%
TTCCTTCCTAAACATGACCA+AGG + chr1_3:64394263-64394282 Msa0133110:exon 40.0%
TCCTTCCTAAACATGACCAA+GGG + chr1_3:64394264-64394283 Msa0133110:exon 40.0%
GTGGTCTTCAAACACTTTCA+CGG + chr1_3:64394297-64394316 Msa0133110:three_prime_UTR 40.0%
TACTTATGGGATTGTTCCAG+AGG + chr1_3:64394483-64394502 Msa0133110:three_prime_UTR 40.0%
TGATTGGCAGCTTCGAACTA+TGG - chr1_3:64393011-64393030 None:intergenic 45.0%
! TGGATGTATTGGTGGCTGAT+TGG - chr1_3:64393027-64393046 None:intergenic 45.0%
!! TAGTAGTAGTGGTGATGGTG+GGG - chr1_3:64393210-64393229 None:intergenic 45.0%
!! CTAGTAGTAGTGGTGATGGT+GGG - chr1_3:64393211-64393230 None:intergenic 45.0%
!! ACTAGTAGTAGTGGTGATGG+TGG - chr1_3:64393212-64393231 None:intergenic 45.0%
ACCATCACCACTACTACTAG+TGG + chr1_3:64393211-64393230 Msa0133110:CDS 45.0%
ACCACTAGTAGTAGTGGTGA+TGG - chr1_3:64393215-64393234 None:intergenic 45.0%
GCAATATGAGGAGGGGAATT+GGG - chr1_3:64393250-64393269 None:intergenic 45.0%
TGCAATATGAGGAGGGGAAT+TGG - chr1_3:64393251-64393270 None:intergenic 45.0%
AGGAAGTGGTAGGGACAAAA+TGG - chr1_3:64393297-64393316 None:intergenic 45.0%
CAAATGGAAGAGGAAGTGGT+AGG - chr1_3:64393307-64393326 None:intergenic 45.0%
CTACCACTTCCTCTTCCATT+TGG + chr1_3:64393305-64393324 Msa0133110:CDS 45.0%
! TGTTTGAGGCTGGATGCTTT+GGG - chr1_3:64393443-64393462 None:intergenic 45.0%
! TTGATCTAGGTGTTTGAGGC+TGG - chr1_3:64393453-64393472 None:intergenic 45.0%
AGAGGTTATCTGCGGTTACA+TGG - chr1_3:64393672-64393691 None:intergenic 45.0%
ATGCCCACAAATCTGTTGAG+AGG - chr1_3:64393690-64393709 None:intergenic 45.0%
TCTCAACAGATTTGTGGGCA+TGG + chr1_3:64393689-64393708 Msa0133110:CDS 45.0%
TTGTGGGCATGGCGAAAATA+TGG + chr1_3:64393700-64393719 Msa0133110:CDS 45.0%
! AGACTTGGTTCGTGTACTTC+CGG - chr1_3:64394092-64394111 None:intergenic 45.0%
AAGTACACGAACCAAGTCTC+CGG + chr1_3:64394093-64394112 Msa0133110:CDS 45.0%
GGGTTGACTAGAAATTGAGG+TGG - chr1_3:64394131-64394150 None:intergenic 45.0%
GCAAGAAGAAGTTGAGCTGT+TGG - chr1_3:64394152-64394171 None:intergenic 45.0%
AGAAGAAGAGGACATCGACA+TGG + chr1_3:64394201-64394220 Msa0133110:CDS 45.0%
TCCCTTGGTCATGTTTAGGA+AGG - chr1_3:64394268-64394287 None:intergenic 45.0%
TCCTAAACATGACCAAGGGA+AGG + chr1_3:64394268-64394287 Msa0133110:exon 45.0%
ACCTTCCCTTGGTCATGTTT+AGG - chr1_3:64394272-64394291 None:intergenic 45.0%
TGATTGGCAGCTTCGAACTA+TGG - chr1_3:64393011-64393030 None:intergenic 45.0%
! TGGATGTATTGGTGGCTGAT+TGG - chr1_3:64393027-64393046 None:intergenic 45.0%
!! TAGTAGTAGTGGTGATGGTG+GGG - chr1_3:64393210-64393229 None:intergenic 45.0%
!! CTAGTAGTAGTGGTGATGGT+GGG - chr1_3:64393211-64393230 None:intergenic 45.0%
!! ACTAGTAGTAGTGGTGATGG+TGG - chr1_3:64393212-64393231 None:intergenic 45.0%
ACCATCACCACTACTACTAG+TGG + chr1_3:64393211-64393230 Msa0133110:CDS 45.0%
ACCACTAGTAGTAGTGGTGA+TGG - chr1_3:64393215-64393234 None:intergenic 45.0%
GCAATATGAGGAGGGGAATT+GGG - chr1_3:64393250-64393269 None:intergenic 45.0%
TGCAATATGAGGAGGGGAAT+TGG - chr1_3:64393251-64393270 None:intergenic 45.0%
AGGAAGTGGTAGGGACAAAA+TGG - chr1_3:64393297-64393316 None:intergenic 45.0%
CAAATGGAAGAGGAAGTGGT+AGG - chr1_3:64393307-64393326 None:intergenic 45.0%
CTACCACTTCCTCTTCCATT+TGG + chr1_3:64393305-64393324 Msa0133110:CDS 45.0%
! TGTTTGAGGCTGGATGCTTT+GGG - chr1_3:64393443-64393462 None:intergenic 45.0%
! TTGATCTAGGTGTTTGAGGC+TGG - chr1_3:64393453-64393472 None:intergenic 45.0%
AGAGGTTATCTGCGGTTACA+TGG - chr1_3:64393672-64393691 None:intergenic 45.0%
ATGCCCACAAATCTGTTGAG+AGG - chr1_3:64393690-64393709 None:intergenic 45.0%
TCTCAACAGATTTGTGGGCA+TGG + chr1_3:64393689-64393708 Msa0133110:CDS 45.0%
TTGTGGGCATGGCGAAAATA+TGG + chr1_3:64393700-64393719 Msa0133110:CDS 45.0%
! AGACTTGGTTCGTGTACTTC+CGG - chr1_3:64394092-64394111 None:intergenic 45.0%
AAGTACACGAACCAAGTCTC+CGG + chr1_3:64394093-64394112 Msa0133110:CDS 45.0%
GGGTTGACTAGAAATTGAGG+TGG - chr1_3:64394131-64394150 None:intergenic 45.0%
GCAAGAAGAAGTTGAGCTGT+TGG - chr1_3:64394152-64394171 None:intergenic 45.0%
AGAAGAAGAGGACATCGACA+TGG + chr1_3:64394201-64394220 Msa0133110:CDS 45.0%
TCCCTTGGTCATGTTTAGGA+AGG - chr1_3:64394268-64394287 None:intergenic 45.0%
TCCTAAACATGACCAAGGGA+AGG + chr1_3:64394268-64394287 Msa0133110:exon 45.0%
ACCTTCCCTTGGTCATGTTT+AGG - chr1_3:64394272-64394291 None:intergenic 45.0%
!! AGTAGTAGTGGTGATGGTGG+GGG - chr1_3:64393209-64393228 None:intergenic 50.0%
AGGCCAAATGGAAGAGGAAG+TGG - chr1_3:64393311-64393330 None:intergenic 50.0%
! GTTTGAGGCTGGATGCTTTG+GGG - chr1_3:64393442-64393461 None:intergenic 50.0%
! GTGTTTGAGGCTGGATGCTT+TGG - chr1_3:64393444-64393463 None:intergenic 50.0%
! CCTTTCAACTTGCTTTCGCG+CGG - chr1_3:64393987-64394006 None:intergenic 50.0%
ACCATGCAAGACCTACCCAT+CGG + chr1_3:64394056-64394075 Msa0133110:CDS 50.0%
!! CATGACCAAGGGAAGGTACT+TGG + chr1_3:64394275-64394294 Msa0133110:three_prime_UTR 50.0%
!! AGTAGTAGTGGTGATGGTGG+GGG - chr1_3:64393209-64393228 None:intergenic 50.0%
AGGCCAAATGGAAGAGGAAG+TGG - chr1_3:64393311-64393330 None:intergenic 50.0%
! GTTTGAGGCTGGATGCTTTG+GGG - chr1_3:64393442-64393461 None:intergenic 50.0%
! GTGTTTGAGGCTGGATGCTT+TGG - chr1_3:64393444-64393463 None:intergenic 50.0%
! CCTTTCAACTTGCTTTCGCG+CGG - chr1_3:64393987-64394006 None:intergenic 50.0%
ACCATGCAAGACCTACCCAT+CGG + chr1_3:64394056-64394075 Msa0133110:CDS 50.0%
!! CATGACCAAGGGAAGGTACT+TGG + chr1_3:64394275-64394294 Msa0133110:three_prime_UTR 50.0%
CTTTCGCGCGGTACAACCTT+TGG - chr1_3:64393975-64393994 None:intergenic 55.0%
! CCGCGCGAAAGCAAGTTGAA+AGG + chr1_3:64393984-64394003 Msa0133110:CDS 55.0%
TCCGATGGGTAGGTCTTGCA+TGG - chr1_3:64394060-64394079 None:intergenic 55.0%
!! GACCAAGGGAAGGTACTTGG+TGG + chr1_3:64394278-64394297 Msa0133110:three_prime_UTR 55.0%
GACCACCAAGTACCTTCCCT+TGG - chr1_3:64394283-64394302 None:intergenic 55.0%
CTTTCGCGCGGTACAACCTT+TGG - chr1_3:64393975-64393994 None:intergenic 55.0%
! CCGCGCGAAAGCAAGTTGAA+AGG + chr1_3:64393984-64394003 Msa0133110:CDS 55.0%
TCCGATGGGTAGGTCTTGCA+TGG - chr1_3:64394060-64394079 None:intergenic 55.0%
!! GACCAAGGGAAGGTACTTGG+TGG + chr1_3:64394278-64394297 Msa0133110:three_prime_UTR 55.0%
GACCACCAAGTACCTTCCCT+TGG - chr1_3:64394283-64394302 None:intergenic 55.0%
GCTAGCGAGTTCCGATGGGT+AGG - chr1_3:64394070-64394089 None:intergenic 60.0%
ACCCATCGGAACTCGCTAGC+CGG + chr1_3:64394070-64394089 Msa0133110:CDS 60.0%
TCCGGCTAGCGAGTTCCGAT+GGG - chr1_3:64394074-64394093 None:intergenic 60.0%
TTCCGGCTAGCGAGTTCCGA+TGG - chr1_3:64394075-64394094 None:intergenic 60.0%
CGGATGTGTCACCGGAGACT+TGG - chr1_3:64394107-64394126 None:intergenic 60.0%
GAGGTGGTCGGATGTGTCAC+CGG - chr1_3:64394115-64394134 None:intergenic 60.0%
GCTAGCGAGTTCCGATGGGT+AGG - chr1_3:64394070-64394089 None:intergenic 60.0%
ACCCATCGGAACTCGCTAGC+CGG + chr1_3:64394070-64394089 Msa0133110:CDS 60.0%
TCCGGCTAGCGAGTTCCGAT+GGG - chr1_3:64394074-64394093 None:intergenic 60.0%
TTCCGGCTAGCGAGTTCCGA+TGG - chr1_3:64394075-64394094 None:intergenic 60.0%
CGGATGTGTCACCGGAGACT+TGG - chr1_3:64394107-64394126 None:intergenic 60.0%
GAGGTGGTCGGATGTGTCAC+CGG - chr1_3:64394115-64394134 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 64392909 64394570 64392909 ID=Msa0133110;Name=Msa0133110
chr1_3 mRNA 64392909 64394570 64392909 ID=Msa0133110-mRNA-1;Parent=Msa0133110;Name=Msa0133110-mRNA-1;_AED=0.07;_eAED=0.07;_QI=74|1|1|1|1|1|3|297|311
chr1_3 exon 64392909 64393479 64392909 ID=Msa0133110-mRNA-1:exon:21657;Parent=Msa0133110-mRNA-1
chr1_3 exon 64393642 64393755 64393642 ID=Msa0133110-mRNA-1:exon:21658;Parent=Msa0133110-mRNA-1
chr1_3 exon 64393949 64394570 64393949 ID=Msa0133110-mRNA-1:exon:21659;Parent=Msa0133110-mRNA-1
chr1_3 five_prime_UTR 64392909 64392982 64392909 ID=Msa0133110-mRNA-1:five_prime_utr;Parent=Msa0133110-mRNA-1
chr1_3 CDS 64392983 64393479 64392983 ID=Msa0133110-mRNA-1:cds;Parent=Msa0133110-mRNA-1
chr1_3 CDS 64393642 64393755 64393642 ID=Msa0133110-mRNA-1:cds;Parent=Msa0133110-mRNA-1
chr1_3 CDS 64393949 64394273 64393949 ID=Msa0133110-mRNA-1:cds;Parent=Msa0133110-mRNA-1
chr1_3 three_prime_UTR 64394274 64394570 64394274 ID=Msa0133110-mRNA-1:three_prime_utr;Parent=Msa0133110-mRNA-1
Gene Sequence

>Msa0133110

ATGGCTGAGGATTGGGATTTGTTTGCCATAGTTCGAAGCTGCCAATCAGCCACCAATACATCCACAACAGAAAATTCATTTACCACGACAAATACTATACCATCTTCCATTTCCTCTTCTAATTTCAAAGTAGAATTAGAATATGATGCATTTTCTTTTCCTAATGTAGTGCAACCTATAACCAATGAGCTTCAAGATCTAAACCAATTATTGACATCTTTTACCCCCACCATCACCACTACTACTAGTGGTCATAGTATCAATCCCAATTCCCCTCCTCATATTGCAAAATACATTGGACAACAACAAAACCATTTTGTCCCTACCACTTCCTCTTCCATTTGGCCTCATTTTGTGCAAGAAACTTCCACTTCTAGCTTCAACATATTCCATAACCAACAATTACAACAACAACAGATTCAACTACAAGCAATTCAGAAACAAGAATTTCAAGTACCCCAAAGCATCCAGCCTCAAACACCTAGATCAAGAAAAAGAAAAAGTCAACAAGGGAAAATGGTGTGCCATGTAACCGCAGATAACCTCTCAACAGATTTGTGGGCATGGCGAAAATATGGACAAAAACCCATTAAAGGTTCTCCACATCCAAGGAACTACTATAGATGTAGCAGTTCCAAAGGTTGTACCGCGCGAAAGCAAGTTGAAAGGAGCAACACTGAAGCAGACATGTTCACTGTGACATATACAGGAGATCATAACCATGCAAGACCTACCCATCGGAACTCGCTAGCCGGAAGTACACGAACCAAGTCTCCGGTGACACATCCGACCACCTCAATTTCTAGTCAACCCAACAGCTCAACTTCTTCTTGCTCCTCTTTAGCACCAAACTCTTTTGGTGAAGAAGAAGAGGACATCGACATGGAAATTGAAACTGATGATGATGGTGAAGATAGTTGTGATATTCCTTCCTAA
Protein sequence

>Msa0133110

MAEDWDLFAIVRSCQSATNTSTTENSFTTTNTIPSSISSSNFKVELEYDAFSFPNVVQPITNELQDLNQLLTSFTPTITTTTSGHSINPNSPPHIAKYIGQQQNHFVPTTSSSIWPHFVQETSTSSFNIFHNQQLQQQQIQLQAIQKQEFQVPQSIQPQTPRSRKRKSQQGKMVCHVTADNLSTDLWAWRKYGQKPIKGSPHPRNYYRCSSSKGCTARKQVERSNTEADMFTVTYTGDHNHARPTHRNSLAGSTRTKSPVTHPTTSISSQPNSSTSSCSSLAPNSFGEEEEDIDMEIETDDDGEDSCDIPS*