AlfalfaGEDB | Msa0134590

M. sativa cultivar zhongmu-4 / Msa0134590

KEGG: -
GO: -
PFAM: PF13302.10

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0134590	sp\|P05332\|YP20_BACLI	34.911	169	93	4	23	175	8	175	2.40e-22	91.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0134590	A0A072VQW9	90.000	190	7	1	1	178	1	190	7.58e-117	338

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TFs/TRs:

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Gene ID	Type	Classification
Msa0134590	TR	GNAT

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0134590	MtrunA17_Chr1g0204951	90.000	190	7	1	1	178	1	190	1.46e-120	338
Msa0134590	MtrunA17_Chr1g0204971	81.579	190	23	1	1	178	1	190	6.82e-110	311
Msa0134590	MtrunA17_Chr5g0394621	63.021	192	55	2	1	178	1	190	3.27e-84	246
Msa0134590	MtrunA17_Chr5g0394631	61.749	183	54	2	1	169	1	181	3.87e-75	230
Msa0134590	MtrunA17_Chr5g0394631	58.919	185	54	6	12	178	221	401	1.94e-65	205
Msa0134590	MtrunA17_Chr7g0273661	47.059	153	70	1	19	160	4	156	5.47e-43	140
Msa0134590	MtrunA17_Chr7g0223351	42.073	164	84	2	22	175	7	169	6.86e-40	133
Msa0134590	MtrunA17_Chr7g0223361	40.000	170	90	2	17	175	2	170	1.23e-38	130

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0134590	AT2G32020.1	53.988	163	66	2	22	175	18	180	1.68e-59	184
Msa0134590	AT2G32030.1	48.913	184	84	3	1	175	3	185	6.60e-59	182
Msa0134590	AT3G22560.1	40.936	171	88	1	18	175	2	172	5.51e-39	131

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Find 36 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAGTTTCCATACCTGGAG+CGG	0.585589	1_3:-66128061	Msa0134590:CDS
AAGTGGCCAAGTATTGCACT+TGG	0.601796	1_3:-66128317	Msa0134590:CDS
AATACCTTTGCCCCAATACT+TGG	0.403153	1_3:+66128124	None:intergenic
ACATAGCAAGCAAATCTCTA+TGG	0.412043	1_3:-66128251	Msa0134590:CDS
ACCCGATTGCTCGATCGCGA+AGG	0.639229	1_3:+66128214	None:intergenic
AGAAAATATCTGTTCATCAA+GGG	0.554840	1_3:-66127957	Msa0134590:CDS
AGAAAATGTGGCTTCTCAGA+GGG	0.613906	1_3:-66128018	Msa0134590:CDS
AGCTCCAAGTATTGGGGCAA+AGG	0.537498	1_3:-66128128	Msa0134590:CDS
AGGAACAAATCTGCTGAACT+TGG	0.442081	1_3:-66128161	Msa0134590:CDS
AGGTTCCCAAGTGCAATACT+TGG	0.527166	1_3:+66128311	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCAAGATCTGAAAGACTAAT+GGG	+	chr1_3:66127986-66128005	None:intergenic	30.0%
ATCAAGATCTGAAAGACTAA+TGG	+	chr1_3:66127987-66128006	None:intergenic	30.0%
CCTTATACAAACAAAGAAGA+CGG	-	chr1_3:66128061-66128080	Msa0134590:CDS	30.0%
ATTTAAAGAGTTTCCATACC+TGG	-	chr1_3:66128286-66128305	Msa0134590:CDS	30.0%
CAGAAAATATCTGTTCATCA+AGG	-	chr1_3:66128394-66128413	Msa0134590:CDS	30.0%
CTCTTCTTTAGCATCTGATT+TGG	+	chr1_3:66127927-66127946	None:intergenic	35.0%
ACATAGCAAGCAAATCTCTA+TGG	-	chr1_3:66128101-66128120	Msa0134590:CDS	35.0%
CAAGGGAAAAAGTAGAGATA+TGG	-	chr1_3:66128412-66128431	Msa0134590:CDS	35.0%
GTGGACAAGTGATGAAAAAG+TGG	-	chr1_3:66128018-66128037	Msa0134590:CDS	40.0%
AGGAACAAATCTGCTGAACT+TGG	-	chr1_3:66128191-66128210	Msa0134590:intron	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_3	gene	66127902	66128472	66127902	ID=Msa0134590;Name=Msa0134590
chr1_3	mRNA	66127902	66128472	66127902	ID=Msa0134590-mRNA-1;Parent=Msa0134590;Name=Msa0134590-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0\|0\|0\|1\|1\|1\|2\|0\|178
chr1_3	exon	66128216	66128472	66128216	ID=Msa0134590-mRNA-1:exon:22546;Parent=Msa0134590-mRNA-1
chr1_3	exon	66127902	66128181	66127902	ID=Msa0134590-mRNA-1:exon:22545;Parent=Msa0134590-mRNA-1
chr1_3	CDS	66128216	66128472	66128216	ID=Msa0134590-mRNA-1:cds;Parent=Msa0134590-mRNA-1
chr1_3	CDS	66127902	66128181	66127902	ID=Msa0134590-mRNA-1:cds;Parent=Msa0134590-mRNA-1

Gene Sequence

>Msa0134590

ATGGAAAAAACTGCTACCTATTCCAAATCAGATGCTAAAGAAGAGTGTTCTGTTGTAGACTTGAGCCAAATATCTCTTCGACCCATTAGTCTTTCAGATCTTGATGATGTTTTGGTGTGGACAAGTGATGAAAAAGTGGCCAAGTATTGCACTTGGGAACCTTATACAAACAAAGAAGACGGCATTGACTTCATCCAAAACATAGCAAGCAAATCTCTATGGTTCAGAGCAATTTGCCTTCGCGATCGAGCAATCGGGAACAAATCTGCTGAACTTGGCTATGCTATGAGCTCCAAGTATTGGGGCAAAGGTATTGCCACACTTGTTGTCAAACAAGTGGTTGAAGCTGCATTTAAAGAGTTTCCATACCTGGAGCGGCTTGAAGCTCAAGTTGATGTAGAAAATGTGGCTTCTCAGAGGGTGTTGGAGAAGGCTGGTTTTCAAAGGGAGGGTGTTCTCAGAAAATATCTGTTCATCAAGGGAAAAAGTAGAGATATGGTTATGTTCAGTATTCTCTCAAGTGAAGTTCAAATTTGA

Protein sequence

>Msa0134590

MEKTATYSKSDAKEECSVVDLSQISLRPISLSDLDDVLVWTSDEKVAKYCTWEPYTNKEDGIDFIQNIASKSLWFRAICLRDRAIGNKSAELGYAMSSKYWGKGIATLVVKQVVEAAFKEFPYLERLEAQVDVENVASQRVLEKAGFQREGVLRKYLFIKGKSRDMVMFSILSSEVQI*