Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0134590 | sp|P05332|YP20_BACLI | 34.911 | 169 | 93 | 4 | 23 | 175 | 8 | 175 | 2.40e-22 | 91.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0134590 | A0A072VQW9 | 90.000 | 190 | 7 | 1 | 1 | 178 | 1 | 190 | 7.58e-117 | 338 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0134590 | TR | GNAT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0134590 | MtrunA17_Chr1g0204951 | 90.000 | 190 | 7 | 1 | 1 | 178 | 1 | 190 | 1.46e-120 | 338 |
| Msa0134590 | MtrunA17_Chr1g0204971 | 81.579 | 190 | 23 | 1 | 1 | 178 | 1 | 190 | 6.82e-110 | 311 |
| Msa0134590 | MtrunA17_Chr5g0394621 | 63.021 | 192 | 55 | 2 | 1 | 178 | 1 | 190 | 3.27e-84 | 246 |
| Msa0134590 | MtrunA17_Chr5g0394631 | 61.749 | 183 | 54 | 2 | 1 | 169 | 1 | 181 | 3.87e-75 | 230 |
| Msa0134590 | MtrunA17_Chr5g0394631 | 58.919 | 185 | 54 | 6 | 12 | 178 | 221 | 401 | 1.94e-65 | 205 |
| Msa0134590 | MtrunA17_Chr7g0273661 | 47.059 | 153 | 70 | 1 | 19 | 160 | 4 | 156 | 5.47e-43 | 140 |
| Msa0134590 | MtrunA17_Chr7g0223351 | 42.073 | 164 | 84 | 2 | 22 | 175 | 7 | 169 | 6.86e-40 | 133 |
| Msa0134590 | MtrunA17_Chr7g0223361 | 40.000 | 170 | 90 | 2 | 17 | 175 | 2 | 170 | 1.23e-38 | 130 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0134590 | AT2G32020.1 | 53.988 | 163 | 66 | 2 | 22 | 175 | 18 | 180 | 1.68e-59 | 184 |
| Msa0134590 | AT2G32030.1 | 48.913 | 184 | 84 | 3 | 1 | 175 | 3 | 185 | 6.60e-59 | 182 |
| Msa0134590 | AT3G22560.1 | 40.936 | 171 | 88 | 1 | 18 | 175 | 2 | 172 | 5.51e-39 | 131 |
Find 36 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTTCTTTAGCATCTGATT+TGG | 0.255230 | 1_3:+66128428 | None:intergenic |
| CCGTCTTCTTTGTTTGTATA+AGG | 0.260514 | 1_3:+66128291 | None:intergenic |
| TGCTATGAGCTCCAAGTATT+GGG | 0.261207 | 1_3:-66128135 | Msa0134590:CDS |
| TTCAGATCTTGATGATGTTT+TGG | 0.285412 | 1_3:-66128358 | Msa0134590:CDS |
| GAGATTTGCTTGCTATGTTT+TGG | 0.329625 | 1_3:+66128256 | None:intergenic |
| TAATGGGTCGAAGAGATATT+TGG | 0.336769 | 1_3:+66128385 | None:intergenic |
| ATCAAGATCTGAAAGACTAA+TGG | 0.348689 | 1_3:+66128368 | None:intergenic |
| TTTAGCATCTGATTTGGAAT+AGG | 0.367598 | 1_3:+66128434 | None:intergenic |
| AATACCTTTGCCCCAATACT+TGG | 0.403153 | 1_3:+66128124 | None:intergenic |
| TGCCTTCGCGATCGAGCAAT+CGG | 0.411073 | 1_3:-66128216 | Msa0134590:CDS |
| CAGAGGGTGTTGGAGAAGGC+TGG | 0.411839 | 1_3:-66128002 | Msa0134590:CDS |
| ACATAGCAAGCAAATCTCTA+TGG | 0.412043 | 1_3:-66128251 | Msa0134590:CDS |
| AGGAACAAATCTGCTGAACT+TGG | 0.442081 | 1_3:-66128161 | Msa0134590:CDS |
| ATGCTATGAGCTCCAAGTAT+TGG | 0.448265 | 1_3:-66128136 | Msa0134590:CDS |
| CTTGAGCTTCAAGCCGCTCC+AGG | 0.458231 | 1_3:+66128048 | None:intergenic |
| TCAAGATCTGAAAGACTAAT+GGG | 0.460408 | 1_3:+66128369 | None:intergenic |
| TTCTCAGAGGGTGTTGGAGA+AGG | 0.470170 | 1_3:-66128006 | Msa0134590:CDS |
| CAGAAAATATCTGTTCATCA+AGG | 0.472630 | 1_3:-66127958 | Msa0134590:CDS |
| GCTTCAAGCCGCTCCAGGTA+TGG | 0.487071 | 1_3:+66128053 | None:intergenic |
| AGGTTCCCAAGTGCAATACT+TGG | 0.527166 | 1_3:+66128311 | None:intergenic |
| AGCTCCAAGTATTGGGGCAA+AGG | 0.537498 | 1_3:-66128128 | Msa0134590:CDS |
| TAGAAAATGTGGCTTCTCAG+AGG | 0.540854 | 1_3:-66128019 | Msa0134590:CDS |
| ATTTAAAGAGTTTCCATACC+TGG | 0.542410 | 1_3:-66128066 | Msa0134590:CDS |
| GCCTTCGCGATCGAGCAATC+GGG | 0.544699 | 1_3:-66128215 | Msa0134590:intron |
| TGTGGCTTCTCAGAGGGTGT+TGG | 0.549890 | 1_3:-66128012 | Msa0134590:CDS |
| AGAAAATATCTGTTCATCAA+GGG | 0.554840 | 1_3:-66127957 | Msa0134590:CDS |
| TCAAGTTGATGTAGAAAATG+TGG | 0.572167 | 1_3:-66128030 | Msa0134590:CDS |
| AAGAGTTTCCATACCTGGAG+CGG | 0.585589 | 1_3:-66128061 | Msa0134590:CDS |
| CACACTTGTTGTCAAACAAG+TGG | 0.596019 | 1_3:-66128099 | Msa0134590:CDS |
| AAGTGGCCAAGTATTGCACT+TGG | 0.601796 | 1_3:-66128317 | Msa0134590:CDS |
| AGAAAATGTGGCTTCTCAGA+GGG | 0.613906 | 1_3:-66128018 | Msa0134590:CDS |
| CACTTGTTTGACAACAAGTG+TGG | 0.622968 | 1_3:+66128100 | None:intergenic |
| CCTTATACAAACAAAGAAGA+CGG | 0.623002 | 1_3:-66128291 | Msa0134590:CDS |
| ACCCGATTGCTCGATCGCGA+AGG | 0.639229 | 1_3:+66128214 | None:intergenic |
| GCTATGAGCTCCAAGTATTG+GGG | 0.656803 | 1_3:-66128134 | Msa0134590:CDS |
| AGTGGCCAAGTATTGCACTT+GGG | 0.670287 | 1_3:-66128316 | Msa0134590:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAAAATATCTGTTCATCAA+GGG | - | chr1_3:66128395-66128414 | Msa0134590:CDS | 25.0% |
| !! | TTTAGCATCTGATTTGGAAT+AGG | + | chr1_3:66127921-66127940 | None:intergenic | 30.0% |
| TCAAGATCTGAAAGACTAAT+GGG | + | chr1_3:66127986-66128005 | None:intergenic | 30.0% | |
| ATCAAGATCTGAAAGACTAA+TGG | + | chr1_3:66127987-66128006 | None:intergenic | 30.0% | |
| !!! | TTCAGATCTTGATGATGTTT+TGG | - | chr1_3:66127994-66128013 | Msa0134590:CDS | 30.0% |
| CCTTATACAAACAAAGAAGA+CGG | - | chr1_3:66128061-66128080 | Msa0134590:CDS | 30.0% | |
| ATTTAAAGAGTTTCCATACC+TGG | - | chr1_3:66128286-66128305 | Msa0134590:CDS | 30.0% | |
| !! | TCAAGTTGATGTAGAAAATG+TGG | - | chr1_3:66128322-66128341 | Msa0134590:CDS | 30.0% |
| CAGAAAATATCTGTTCATCA+AGG | - | chr1_3:66128394-66128413 | Msa0134590:CDS | 30.0% | |
| CTCTTCTTTAGCATCTGATT+TGG | + | chr1_3:66127927-66127946 | None:intergenic | 35.0% | |
| ! | TAATGGGTCGAAGAGATATT+TGG | + | chr1_3:66127970-66127989 | None:intergenic | 35.0% |
| !!! | ATCTTGATGATGTTTTGGTG+TGG | - | chr1_3:66127999-66128018 | Msa0134590:CDS | 35.0% |
| ! | CCGTCTTCTTTGTTTGTATA+AGG | + | chr1_3:66128064-66128083 | None:intergenic | 35.0% |
| !!! | GAGATTTGCTTGCTATGTTT+TGG | + | chr1_3:66128099-66128118 | None:intergenic | 35.0% |
| ACATAGCAAGCAAATCTCTA+TGG | - | chr1_3:66128101-66128120 | Msa0134590:CDS | 35.0% | |
| !! | TGTATCGATTTCTCTGTGTT+CGG | - | chr1_3:66128160-66128179 | Msa0134590:CDS | 35.0% |
| CAAGGGAAAAAGTAGAGATA+TGG | - | chr1_3:66128412-66128431 | Msa0134590:CDS | 35.0% | |
| GTGGACAAGTGATGAAAAAG+TGG | - | chr1_3:66128018-66128037 | Msa0134590:CDS | 40.0% | |
| AGGAACAAATCTGCTGAACT+TGG | - | chr1_3:66128191-66128210 | Msa0134590:intron | 40.0% | |
| ATGCTATGAGCTCCAAGTAT+TGG | - | chr1_3:66128216-66128235 | Msa0134590:CDS | 40.0% | |
| TGCTATGAGCTCCAAGTATT+GGG | - | chr1_3:66128217-66128236 | Msa0134590:CDS | 40.0% | |
| AATACCTTTGCCCCAATACT+TGG | + | chr1_3:66128231-66128250 | None:intergenic | 40.0% | |
| CACTTGTTTGACAACAAGTG+TGG | + | chr1_3:66128255-66128274 | None:intergenic | 40.0% | |
| !! | CACACTTGTTGTCAAACAAG+TGG | - | chr1_3:66128253-66128272 | Msa0134590:CDS | 40.0% |
| TAGAAAATGTGGCTTCTCAG+AGG | - | chr1_3:66128333-66128352 | Msa0134590:CDS | 40.0% | |
| AGAAAATGTGGCTTCTCAGA+GGG | - | chr1_3:66128334-66128353 | Msa0134590:CDS | 40.0% | |
| ! | AAGTGGCCAAGTATTGCACT+TGG | - | chr1_3:66128035-66128054 | Msa0134590:CDS | 45.0% |
| ! | AGTGGCCAAGTATTGCACTT+GGG | - | chr1_3:66128036-66128055 | Msa0134590:CDS | 45.0% |
| AGGTTCCCAAGTGCAATACT+TGG | + | chr1_3:66128044-66128063 | None:intergenic | 45.0% | |
| ! | GCTATGAGCTCCAAGTATTG+GGG | - | chr1_3:66128218-66128237 | Msa0134590:CDS | 45.0% |
| AAGAGTTTCCATACCTGGAG+CGG | - | chr1_3:66128291-66128310 | Msa0134590:CDS | 45.0% | |
| !!! | TGGAGAAGGCTGGTTTTCAA+AGG | - | chr1_3:66128360-66128379 | Msa0134590:CDS | 45.0% |
| !!! | GGAGAAGGCTGGTTTTCAAA+GGG | - | chr1_3:66128361-66128380 | Msa0134590:CDS | 45.0% |
| !!! | AAGGCTGGTTTTCAAAGGGA+GGG | - | chr1_3:66128365-66128384 | Msa0134590:CDS | 45.0% |
| CTCTGTGTTCGGATAGATGC+AGG | - | chr1_3:66128171-66128190 | Msa0134590:intron | 50.0% | |
| !! | AGCTCCAAGTATTGGGGCAA+AGG | - | chr1_3:66128224-66128243 | Msa0134590:CDS | 50.0% |
| !! | TTCTCAGAGGGTGTTGGAGA+AGG | - | chr1_3:66128346-66128365 | Msa0134590:CDS | 50.0% |
| !!! | GAAGGCTGGTTTTCAAAGGG+AGG | - | chr1_3:66128364-66128383 | Msa0134590:CDS | 50.0% |
| TGCCTTCGCGATCGAGCAAT+CGG | - | chr1_3:66128136-66128155 | Msa0134590:CDS | 55.0% | |
| ! | TGTGGCTTCTCAGAGGGTGT+TGG | - | chr1_3:66128340-66128359 | Msa0134590:CDS | 55.0% |
| GCCTTCGCGATCGAGCAATC+GGG | - | chr1_3:66128137-66128156 | Msa0134590:CDS | 60.0% | |
| ACCCGATTGCTCGATCGCGA+AGG | + | chr1_3:66128141-66128160 | None:intergenic | 60.0% | |
| GCTTCAAGCCGCTCCAGGTA+TGG | + | chr1_3:66128302-66128321 | None:intergenic | 60.0% | |
| CTTGAGCTTCAAGCCGCTCC+AGG | + | chr1_3:66128307-66128326 | None:intergenic | 60.0% | |
| !! | CAGAGGGTGTTGGAGAAGGC+TGG | - | chr1_3:66128350-66128369 | Msa0134590:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_3 | gene | 66127902 | 66128472 | 66127902 | ID=Msa0134590;Name=Msa0134590 |
| chr1_3 | mRNA | 66127902 | 66128472 | 66127902 | ID=Msa0134590-mRNA-1;Parent=Msa0134590;Name=Msa0134590-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0|0|0|1|1|1|2|0|178 |
| chr1_3 | exon | 66128216 | 66128472 | 66128216 | ID=Msa0134590-mRNA-1:exon:22546;Parent=Msa0134590-mRNA-1 |
| chr1_3 | exon | 66127902 | 66128181 | 66127902 | ID=Msa0134590-mRNA-1:exon:22545;Parent=Msa0134590-mRNA-1 |
| chr1_3 | CDS | 66128216 | 66128472 | 66128216 | ID=Msa0134590-mRNA-1:cds;Parent=Msa0134590-mRNA-1 |
| chr1_3 | CDS | 66127902 | 66128181 | 66127902 | ID=Msa0134590-mRNA-1:cds;Parent=Msa0134590-mRNA-1 |
| Gene Sequence |
| Protein sequence |
