AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0134670

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0134670 MtrunA17_Chr1g0205031 87.993 558 56 3 1 557 1 548 0.0 996
Msa0134670 MtrunA17_Chr5g0394661 34.652 632 272 17 16 543 23 617 7.14e-80 266
Msa0134670 MtrunA17_Chr1g0205021 40.196 306 79 8 16 294 2 230 6.21e-49 170
Msa0134670 MtrunA17_Chr1g0205011 51.389 216 69 6 235 429 4 204 5.76e-45 158
Msa0134670 MtrunA17_Chr1g0149101 37.415 147 88 4 415 558 500 645 9.91e-24 105
Msa0134670 MtrunA17_Chr8g0390351 37.594 133 73 4 415 543 85 211 1.26e-17 86.3
Msa0134670 MtrunA17_Chr6g0485811 39.516 124 65 4 424 543 139 256 2.08e-17 85.9
Msa0134670 MtrunA17_Chr1g0205001 40.132 152 71 7 189 327 18 162 6.52e-15 74.3
Msa0134670 MtrunA17_Chr1g0205001 73.810 42 11 0 15 56 61 102 1.01e-13 70.9
Msa0134670 MtrunA17_Chr1g0205001 58.491 53 22 0 408 460 168 220 2.44e-11 63.9
Msa0134670 MtrunA17_Chr3g0139951 36.641 131 69 6 417 537 143 269 1.93e-14 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0134670 AT4G14770.1 29.152 566 296 24 9 543 13 504 2.30e-41 159
Msa0134670 AT4G14770.3 28.853 558 293 23 16 543 2 485 6.96e-41 157
Msa0134670 AT4G14770.2 28.905 557 292 23 17 543 2 484 1.15e-40 156
Msa0134670 AT3G22760.1 27.486 553 284 17 16 555 21 469 1.31e-40 156
Msa0134670 AT3G22760.2 31.889 323 170 7 233 555 74 346 5.83e-39 149
Msa0134670 AT3G04850.1 33.101 287 135 9 266 537 332 576 1.11e-30 127
Msa0134670 AT5G25790.3 38.333 120 68 3 424 543 106 219 1.60e-17 85.9
Msa0134670 AT5G25790.2 35.833 120 75 2 424 543 106 223 1.13e-16 83.6
Msa0134670 AT5G25790.1 35.833 120 75 2 424 543 106 223 1.68e-16 82.8
Msa0134670 AT2G20110.3 38.583 127 68 4 415 537 121 241 1.25e-15 80.5
Msa0134670 AT2G20110.2 38.583 127 68 4 415 537 121 241 1.31e-15 80.5
Msa0134670 AT2G20110.1 38.583 127 68 4 415 537 121 241 1.42e-15 80.1
Msa0134670 AT4G29000.1 34.884 129 82 2 415 543 134 260 2.05e-15 79.7
Msa0134670 AT3G16160.1 38.211 123 67 5 415 535 68 183 5.42e-12 68.2

Find 97 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTTGCCAATGTTGCTTTAA+TGG 0.092114 1_3:-66198993 Msa0134670:CDS
TATGCCTTCGTAATGATTTA+AGG 0.098318 1_3:+66198140 None:intergenic
CCAGAATTCTTTCGAACTTT+AGG 0.204484 1_3:+66197083 None:intergenic
CCTAAAGTTCGAAAGAATTC+TGG 0.241815 1_3:-66197083 Msa0134670:CDS
GACGAGTGAAGTGCAATTTA+AGG 0.271122 1_3:-66197914 Msa0134670:CDS
TTGCACTTCACTCGTCCTTT+TGG 0.274158 1_3:+66197921 None:intergenic
CTTTGAAGCTGGAGCTTTGT+TGG 0.274516 1_3:+66196950 None:intergenic
TCGATTGATCTAGTTTCTTC+TGG 0.291859 1_3:-66197829 Msa0134670:CDS
TAAGTGCAGCTCGGTGATTC+AGG 0.293566 1_3:+66198087 None:intergenic
TTTGAAGCTGGAGCTTTGTT+GGG 0.308021 1_3:+66196951 None:intergenic
ACTGTTGACCCACTTGCTTT+TGG 0.308321 1_3:-66199088 Msa0134670:CDS
TTTCTGTCTGTTGCGGCTAT+TGG 0.313269 1_3:+66199031 None:intergenic
TTGAAGGTGGAACATTTATT+CGG 0.330302 1_3:-66199207 Msa0134670:CDS
TCACAGATAAAGCTACTAAT+TGG 0.348112 1_3:-66196666 Msa0134670:CDS
CACCCTTCACATTTACAACT+TGG 0.349859 1_3:+66196756 None:intergenic
GAATATGGACTCGTCATTAA+AGG 0.354050 1_3:+66199547 None:intergenic
CAAGTACCTCGTGAGGAATA+TGG 0.357398 1_3:+66199532 None:intergenic
CACCAATGGAGACACATTTA+CGG 0.379389 1_3:-66198014 Msa0134670:CDS
CTGCGTTGTCGCTTTGAAGC+TGG 0.389538 1_3:+66196939 None:intergenic
ACAGCCTCAGATGAAAGTTC+AGG 0.391141 1_3:+66199406 None:intergenic
GCATCATCACTTGAGATTTG+AGG 0.396600 1_3:+66199307 None:intergenic
AGAGTTGTTCCTTTGCATAC+TGG 0.415880 1_3:+66198251 None:intergenic
AAGTCATCAGTTCACATTCC+TGG 0.424450 1_3:-66197967 Msa0134670:CDS
AATTGTGGCTGCTTTGCTGC+TGG 0.427566 1_3:-66197221 Msa0134670:CDS
AAGTATCTTCTTGAATTACT+TGG 0.428004 1_3:-66199283 Msa0134670:CDS
AAGTTATTGTAATCACAATC+TGG 0.434332 1_3:+66199178 None:intergenic
ACCCTTCACATTTACAACTT+GGG 0.434704 1_3:+66196757 None:intergenic
TGAAGGTGGAACATTTATTC+GGG 0.437173 1_3:-66199206 Msa0134670:CDS
TAGTTTCTGACCACAATTTG+AGG 0.447183 1_3:+66197053 None:intergenic
CCCTTCACATTTACAACTTG+GGG 0.459373 1_3:+66196758 None:intergenic
TTGAATATTTCTGTAAGTCA+AGG 0.480505 1_3:-66198060 Msa0134670:CDS
AGTTAGATCAAAACATGAAA+AGG 0.487287 1_3:-66197876 Msa0134670:CDS
TTCTGGTTCAGAACATGTAA+AGG 0.491375 1_3:-66197812 Msa0134670:CDS
TCTTGTACATCCGTACCATC+TGG 0.501343 1_3:+66199250 None:intergenic
CCTTCGTAATGATTTAAGGC+TGG 0.512377 1_3:+66198144 None:intergenic
GATATCCATTAAAGCAACAT+TGG 0.513739 1_3:+66198988 None:intergenic
TCTGTCAGAGTATCCGCAAA+AGG 0.520220 1_3:-66197771 Msa0134670:CDS
TGGTTCCTCACCTCAAATTG+TGG 0.524835 1_3:-66197063 Msa0134670:intron
CGAACTTTAGGAACAAATGC+AGG 0.526329 1_3:+66197095 None:intergenic
AACAGACAGAAATCAAGCAC+AGG 0.530854 1_3:-66199020 Msa0134670:CDS
CGAAAATATACATGGTAGGA+AGG 0.533470 1_3:-66196733 Msa0134670:CDS
TGAATATTTCTGTAAGTCAA+GGG 0.533893 1_3:-66198059 Msa0134670:CDS
GTTGCGGCTATTGGTTCTGA+AGG 0.534364 1_3:+66199040 None:intergenic
TGCTTGATTTCTGTCTGTTG+CGG 0.536356 1_3:+66199024 None:intergenic
ATCTCAAGTCTCTGCACCAA+TGG 0.537204 1_3:-66198028 Msa0134670:CDS
CACACTTCTGTGAATCACTA+GGG 0.539386 1_3:+66198202 None:intergenic
AGAGTATCCGCAAAAGGAAG+AGG 0.539876 1_3:-66197765 Msa0134670:CDS
TTGAAGCTGGAGCTTTGTTG+GGG 0.541047 1_3:+66196952 None:intergenic
TTAAAGGAGATAGAGTATTG+AGG 0.545460 1_3:+66199657 None:intergenic
CCTCTTGTGAGTTGGAAACA+AGG 0.546159 1_3:+66199917 None:intergenic
TTCAGAACATGTAAAGGAAG+AGG 0.548580 1_3:-66197806 Msa0134670:CDS
ATACAGTTCTGCAAGCTCGC+AGG 0.548799 1_3:-66197139 Msa0134670:CDS
CCTTGTTTCCAACTCACAAG+AGG 0.548913 1_3:-66199917 Msa0134670:intron
AAGTAATTCAAGAAGATACT+TGG 0.551163 1_3:+66199285 None:intergenic
ACACACTTCTGTGAATCACT+AGG 0.552964 1_3:+66198201 None:intergenic
ACTATGACGCTGAACTCACT+TGG 0.558600 1_3:-66199605 Msa0134670:CDS
ATGAAAAGATGTCTTCTCAA+AGG 0.565868 1_3:-66198113 Msa0134670:CDS
GCTGCTGGAGTCTACTGCAC+AGG 0.566140 1_3:-66197206 Msa0134670:CDS
GCAGAATACGATGCAAGTGC+AGG 0.569764 1_3:-66196920 Msa0134670:CDS
CTCTGACAGAAATCTTCACC+AGG 0.570362 1_3:+66197949 None:intergenic
AAACATGAAAAGGACGATGA+AGG 0.570624 1_3:-66197866 Msa0134670:CDS
AGCCGTAAATGTGTCTCCAT+TGG 0.572557 1_3:+66198012 None:intergenic
TCTGTCAGAGTACTCCCAAA+AGG 0.572781 1_3:-66197936 Msa0134670:CDS
CTCTTGTGAGTTGGAAACAA+GGG 0.576484 1_3:+66199918 None:intergenic
GACGCTGAACTCACTTGGCA+TGG 0.578891 1_3:-66199600 Msa0134670:CDS
CTACAGATTCAACTCATGCA+TGG 0.581951 1_3:-66199354 Msa0134670:CDS
CAAGCAAGAACCAGATGGTA+CGG 0.582545 1_3:-66199260 Msa0134670:CDS
AGTATACAGATGAGCTGTCA+AGG 0.583545 1_3:-66198291 Msa0134670:CDS
AAATCCTGAACTTTCATCTG+AGG 0.591883 1_3:-66199410 Msa0134670:CDS
GGTGGAACATTTATTCGGGA+AGG 0.592387 1_3:-66199202 Msa0134670:CDS
ACATCGAGTGATGTGAAAAG+AGG 0.592896 1_3:+66199572 None:intergenic
TTCAAAGAATAAGTGCAGCT+CGG 0.593807 1_3:+66198078 None:intergenic
ACGATGAAGGTGCATAGGAA+AGG 0.596875 1_3:-66197853 Msa0134670:CDS
GATATCAATCAAATTAAGAG+AGG 0.597084 1_3:-66198971 Msa0134670:CDS
AAAGGACGATGAAGGTGCAT+AGG 0.604624 1_3:-66197858 Msa0134670:CDS
TAACTATTACTGGTAGATTG+TGG 0.605161 1_3:+66199124 None:intergenic
TCTGACCACAATTTGAGGTG+AGG 0.607327 1_3:+66197058 None:intergenic
GTCACAGATGCAGTTGAAGG+TGG 0.608535 1_3:-66199220 Msa0134670:CDS
TCTGTCACAGATGCAGTTGA+AGG 0.611775 1_3:-66199223 Msa0134670:CDS
TCCCCAAGTTGTAAATGTGA+AGG 0.615749 1_3:-66196759 Msa0134670:CDS
CATGCATGAGTTGAATCTGT+AGG 0.615864 1_3:+66199355 None:intergenic
GGTGCGAAAATATACATGGT+AGG 0.616427 1_3:-66196737 Msa0134670:CDS
ACTGGTAGATTGTGGTAACA+TGG 0.617815 1_3:+66199132 None:intergenic
ATATCAATCAAATTAAGAGA+GGG 0.618437 1_3:-66198970 Msa0134670:CDS
AGGAATGTGAACTGATGACT+TGG 0.627401 1_3:+66197969 None:intergenic
ACTTTAGGAACAAATGCAGG+TGG 0.632470 1_3:+66197098 None:intergenic
TATCAATCAAATTAAGAGAG+GGG 0.636347 1_3:-66198969 Msa0134670:CDS
AAAGGAACAACTCTGATGAG+AGG 0.637830 1_3:-66198242 Msa0134670:CDS
AATTAAGAGAGGGGATGATG+AGG 0.639810 1_3:-66198960 Msa0134670:intron
CTGAGATGACCAGTATGCAA+AGG 0.640258 1_3:-66198260 Msa0134670:CDS
GAAGGGTGCGAAAATATACA+TGG 0.643125 1_3:-66196741 Msa0134670:CDS
ATTGGTGCAGAGACTTGAGA+TGG 0.644312 1_3:+66198030 None:intergenic
CTTGGCAAGCAAGAACCAGA+TGG 0.652633 1_3:-66199265 Msa0134670:CDS
AAGCAGTCTTGACATGAGCA+AGG 0.654291 1_3:+66197182 None:intergenic
CCCCAAGTTGTAAATGTGAA+GGG 0.657560 1_3:-66196758 Msa0134670:CDS
CCAGCCTTAAATCATTACGA+AGG 0.687773 1_3:-66198144 Msa0134670:CDS
ACGAGTCCATATTCCTCACG+AGG 0.727777 1_3:-66199538 Msa0134670:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAACTAACATATAATAA+TGG + chr1_3:66198910-66198929 None:intergenic 10.0%
!! AATATCAATATCATTAATAT+AGG + chr1_3:66199615-66199634 None:intergenic 10.0%
!! TATAATAATATGAATTACAA+TGG - chr1_3:66199788-66199807 Msa0134670:intron 10.0%
!! ATAATAATATGAATTACAAT+GGG - chr1_3:66199789-66199808 Msa0134670:intron 10.0%
!!! ATTTTTCATATTTTTATTGC+AGG - chr1_3:66196903-66196922 Msa0134670:CDS 15.0%
!!! CATTTTATAATAACTATTAC+TGG + chr1_3:66197491-66197510 None:intergenic 15.0%
!! TTAGAATGAATAATTTGATT+AGG - chr1_3:66197849-66197868 Msa0134670:CDS 15.0%
!!! TGAAAGTAATTTTATATCTA+GGG + chr1_3:66199196-66199215 None:intergenic 15.0%
!! AATAAAAACCAAGAAAATCA+AGG + chr1_3:66196783-66196802 None:intergenic 20.0%
!! ATATCAATCAAATTAAGAGA+GGG - chr1_3:66197632-66197651 Msa0134670:intron 20.0%
!!! ATAAAACTTGTTCCTTTTTT+TGG - chr1_3:66199052-66199071 Msa0134670:CDS 20.0%
!!! TAAAACTTGTTCCTTTTTTT+GGG - chr1_3:66199053-66199072 Msa0134670:CDS 20.0%
!!! GAAAGTAATTTTATATCTAG+GGG + chr1_3:66199195-66199214 None:intergenic 20.0%
!!! GTGAAAGTAATTTTATATCT+AGG + chr1_3:66199197-66199216 None:intergenic 20.0%
!!! ATTTAAGCTTTGACAATAAT+TGG - chr1_3:66199304-66199323 Msa0134670:CDS 20.0%
!!! TTTAAGCTTTGACAATAATT+GGG - chr1_3:66199305-66199324 Msa0134670:CDS 20.0%
!!! AAACAAAAGGTTAATTTGAA+AGG + chr1_3:66199338-66199357 None:intergenic 20.0%
!! AAGCAAAAAAAGTAAACAAA+AGG + chr1_3:66199351-66199370 None:intergenic 20.0%
!! CATACATAATATGTAAACAA+TGG + chr1_3:66199754-66199773 None:intergenic 20.0%
! ATGGAGATTAATCATATCTA+AGG + chr1_3:66196863-66196882 None:intergenic 25.0%
! TTAGATATGATTAATCTCCA+TGG - chr1_3:66196862-66196881 Msa0134670:intron 25.0%
! TAAATGCAATAAGAATACCA+TGG + chr1_3:66196882-66196901 None:intergenic 25.0%
!!! ACATACTTTTTGGTGTTTAA+AGG + chr1_3:66196964-66196983 None:intergenic 25.0%
!! TTAAACACCAAAAAGTATGT+TGG - chr1_3:66196964-66196983 Msa0134670:intron 25.0%
! AAGTAATTCAAGAAGATACT+TGG + chr1_3:66197320-66197339 None:intergenic 25.0%
! AAGTATCTTCTTGAATTACT+TGG - chr1_3:66197319-66197338 Msa0134670:intron 25.0%
! AAGTTATTGTAATCACAATC+TGG + chr1_3:66197427-66197446 None:intergenic 25.0%
! GATATCAATCAAATTAAGAG+AGG - chr1_3:66197631-66197650 Msa0134670:intron 25.0%
! TATCAATCAAATTAAGAGAG+GGG - chr1_3:66197633-66197652 Msa0134670:intron 25.0%
!!! TCAGTTTAACCTGTTATTTT+AGG - chr1_3:66198049-66198068 Msa0134670:CDS 25.0%
!!! TTTAACCTGTTATTTTAGGT+GGG - chr1_3:66198053-66198072 Msa0134670:CDS 25.0%
! TTACGACTCTTTCTATTAAA+CGG + chr1_3:66198120-66198139 None:intergenic 25.0%
!! TTGAATATTTCTGTAAGTCA+AGG - chr1_3:66198542-66198561 Msa0134670:intron 25.0%
!! TGAATATTTCTGTAAGTCAA+GGG - chr1_3:66198543-66198562 Msa0134670:intron 25.0%
! AGTTAGATCAAAACATGAAA+AGG - chr1_3:66198726-66198745 Msa0134670:intron 25.0%
! GATATTGATATTCTTCTTGT+AGG - chr1_3:66199623-66199642 Msa0134670:CDS 25.0%
!! TAAACGTTGTGAATGTTTTA+AGG - chr1_3:66199722-66199741 Msa0134670:intron 25.0%
! ATAATATGAATTACAATGGG+TGG - chr1_3:66199792-66199811 Msa0134670:intron 25.0%
!! AAGAAAATCAAGGTTGATAG+AGG + chr1_3:66196773-66196792 None:intergenic 30.0%
! TCTATCAACCTTGATTTTCT+TGG - chr1_3:66196772-66196791 Msa0134670:CDS 30.0%
!! TTAAAGGAGATAGAGTATTG+AGG + chr1_3:66196948-66196967 None:intergenic 30.0%
TCTCTCTATTATTCATTGCT+AGG - chr1_3:66197149-66197168 Msa0134670:CDS 30.0%
TTGAAGGTGGAACATTTATT+CGG - chr1_3:66197395-66197414 Msa0134670:intron 30.0%
TAACTATTACTGGTAGATTG+TGG + chr1_3:66197481-66197500 None:intergenic 30.0%
GATATCCATTAAAGCAACAT+TGG + chr1_3:66197617-66197636 None:intergenic 30.0%
CAGAATGGCATAAAAAATAC+AGG + chr1_3:66197795-66197814 None:intergenic 30.0%
TGGTCATATAAATATGCAGA+AGG + chr1_3:66197892-66197911 None:intergenic 30.0%
AATTGCAATTCTAACAGACA+TGG + chr1_3:66197912-66197931 None:intergenic 30.0%
TTGCAATTTAACACTGTTGT+TGG - chr1_3:66197924-66197943 Msa0134670:CDS 30.0%
CTTGGTTATATTGTATCTTG+TGG - chr1_3:66197953-66197972 Msa0134670:CDS 30.0%
CTGTACTTTCAAAGTAACAA+TGG + chr1_3:66197987-66198006 None:intergenic 30.0%
AAGTACAGTCTATACAAAGT+AGG - chr1_3:66197999-66198018 Msa0134670:CDS 30.0%
AGTACAGTCTATACAAAGTA+GGG - chr1_3:66198000-66198019 Msa0134670:CDS 30.0%
AACAGGTTAAACTGATATGT+TGG + chr1_3:66198044-66198063 None:intergenic 30.0%
!!! GTTTAACCTGTTATTTTAGG+TGG - chr1_3:66198052-66198071 Msa0134670:CDS 30.0%
!!! TTAACCTGTTATTTTAGGTG+GGG - chr1_3:66198054-66198073 Msa0134670:CDS 30.0%
TACGACTCTTTCTATTAAAC+GGG + chr1_3:66198119-66198138 None:intergenic 30.0%
TACTCTATTATGACATGCAA+TGG + chr1_3:66198158-66198177 None:intergenic 30.0%
TATGCCTTCGTAATGATTTA+AGG + chr1_3:66198465-66198484 None:intergenic 30.0%
ATGAAAAGATGTCTTCTCAA+AGG - chr1_3:66198489-66198508 Msa0134670:intron 30.0%
GAATAAAGATTATCAAGCAG+AGG + chr1_3:66198888-66198907 None:intergenic 30.0%
!! TTACCTTCATTTTAGTAAGC+TGG - chr1_3:66199115-66199134 Msa0134670:CDS 30.0%
AAACCAGCTTACTAAAATGA+AGG + chr1_3:66199121-66199140 None:intergenic 30.0%
TTTATATCTAGGGGATGTAA+AGG + chr1_3:66199186-66199205 None:intergenic 30.0%
!!! TGCTTCTTTTTCAGTAATTG+TGG - chr1_3:66199366-66199385 Msa0134670:CDS 30.0%
!!! AATGGGTGGTTTTATTTTGA+AGG - chr1_3:66199806-66199825 Msa0134670:intron 30.0%
!!! ATGGGTGGTTTTATTTTGAA+GGG - chr1_3:66199807-66199826 Msa0134670:intron 30.0%
TCACAGATAAAGCTACTAAT+TGG - chr1_3:66199936-66199955 Msa0134670:CDS 30.0%
AAATGCAGCATTGTGATACA+AGG + chr1_3:66196717-66196736 None:intergenic 35.0%
! ACCAAAAAGTATGTTGGAGT+TGG - chr1_3:66196970-66196989 Msa0134670:intron 35.0%
! TCCAACTCCAACATACTTTT+TGG + chr1_3:66196974-66196993 None:intergenic 35.0%
GAATATGGACTCGTCATTAA+AGG + chr1_3:66197058-66197077 None:intergenic 35.0%
! ACGAGGTACTTGTAAAATCT+TGG - chr1_3:66197081-66197100 Msa0134670:CDS 35.0%
AAATCCTGAACTTTCATCTG+AGG - chr1_3:66197192-66197211 Msa0134670:CDS 35.0%
TGAAGGTGGAACATTTATTC+GGG - chr1_3:66197396-66197415 Msa0134670:intron 35.0%
!! GTTTTGAACCAAAAGCAAGT+GGG + chr1_3:66197525-66197544 None:intergenic 35.0%
!! TGTTTTGAACCAAAAGCAAG+TGG + chr1_3:66197526-66197545 None:intergenic 35.0%
!! TCTTGCCAATGTTGCTTTAA+TGG - chr1_3:66197609-66197628 Msa0134670:intron 35.0%
!! AATGGATTACACGATCAGAA+TGG + chr1_3:66197810-66197829 None:intergenic 35.0%
CAGTAACTCAAACTTGTGAA+TGG + chr1_3:66197828-66197847 None:intergenic 35.0%
GTACAGTCTATACAAAGTAG+GGG - chr1_3:66198001-66198020 Msa0134670:CDS 35.0%
! AAGTGTGTTTTGCAAGGAAA+TGG - chr1_3:66198416-66198435 Msa0134670:intron 35.0%
! TGCAAGGAAATGGTTTTTAC+TGG - chr1_3:66198426-66198445 Msa0134670:intron 35.0%
TTCAAAGAATAAGTGCAGCT+CGG + chr1_3:66198527-66198546 None:intergenic 35.0%
AAACATGAAAAGGACGATGA+AGG - chr1_3:66198736-66198755 Msa0134670:intron 35.0%
! TCGATTGATCTAGTTTCTTC+TGG - chr1_3:66198773-66198792 Msa0134670:intron 35.0%
!! TTCTGGTTCAGAACATGTAA+AGG - chr1_3:66198790-66198809 Msa0134670:intron 35.0%
TTCAGAACATGTAAAGGAAG+AGG - chr1_3:66198796-66198815 Msa0134670:intron 35.0%
TTGTGCTATCACCCAAAAAA+AGG + chr1_3:66199067-66199086 None:intergenic 35.0%
CCAGAATTCTTTCGAACTTT+AGG + chr1_3:66199522-66199541 None:intergenic 35.0%
CCTAAAGTTCGAAAGAATTC+TGG - chr1_3:66199519-66199538 Msa0134670:intron 35.0%
TAGTTTCTGACCACAATTTG+AGG + chr1_3:66199552-66199571 None:intergenic 35.0%
ACCCTTCACATTTACAACTT+GGG + chr1_3:66199848-66199867 None:intergenic 35.0%
CGAAAATATACATGGTAGGA+AGG - chr1_3:66199869-66199888 Msa0134670:intron 35.0%
! CTCTTGTGAGTTGGAAACAA+GGG + chr1_3:66196687-66196706 None:intergenic 40.0%
GGAAAAAACCTCTTGTGAGT+TGG + chr1_3:66196696-66196715 None:intergenic 40.0%
!! GGTTGATAGAGGAAAGAGAA+AGG + chr1_3:66196762-66196781 None:intergenic 40.0%
ACATCGAGTGATGTGAAAAG+AGG + chr1_3:66197033-66197052 None:intergenic 40.0%
! GATTTTACAAGTACCTCGTG+AGG + chr1_3:66197080-66197099 None:intergenic 40.0%
! CATGCATGAGTTGAATCTGT+AGG + chr1_3:66197250-66197269 None:intergenic 40.0%
CTACAGATTCAACTCATGCA+TGG - chr1_3:66197248-66197267 Msa0134670:intron 40.0%
GCATCATCACTTGAGATTTG+AGG + chr1_3:66197298-66197317 None:intergenic 40.0%
ACTGGTAGATTGTGGTAACA+TGG + chr1_3:66197473-66197492 None:intergenic 40.0%
TGCTTGATTTCTGTCTGTTG+CGG + chr1_3:66197581-66197600 None:intergenic 40.0%
AACAGACAGAAATCAAGCAC+AGG - chr1_3:66197582-66197601 Msa0134670:intron 40.0%
AATTAAGAGAGGGGATGATG+AGG - chr1_3:66197642-66197661 Msa0134670:intron 40.0%
TTCACCCCACCTAAAATAAC+AGG + chr1_3:66198061-66198080 None:intergenic 40.0%
!! ATTTTAGGTGGGGTGAATTG+AGG - chr1_3:66198064-66198083 Msa0134670:CDS 40.0%
AGTATACAGATGAGCTGTCA+AGG - chr1_3:66198311-66198330 Msa0134670:CDS 40.0%
AGAGTTGTTCCTTTGCATAC+TGG + chr1_3:66198354-66198373 None:intergenic 40.0%
AAAGGAACAACTCTGATGAG+AGG - chr1_3:66198360-66198379 Msa0134670:intron 40.0%
CACACTTCTGTGAATCACTA+GGG + chr1_3:66198403-66198422 None:intergenic 40.0%
ACACACTTCTGTGAATCACT+AGG + chr1_3:66198404-66198423 None:intergenic 40.0%
!! TCACAGAAGTGTGTTTTGCA+AGG - chr1_3:66198410-66198429 Msa0134670:intron 40.0%
! CCTTCGTAATGATTTAAGGC+TGG + chr1_3:66198461-66198480 None:intergenic 40.0%
CCAGCCTTAAATCATTACGA+AGG - chr1_3:66198458-66198477 Msa0134670:intron 40.0%
CACCAATGGAGACACATTTA+CGG - chr1_3:66198588-66198607 Msa0134670:intron 40.0%
AGGAATGTGAACTGATGACT+TGG + chr1_3:66198636-66198655 None:intergenic 40.0%
AAGTCATCAGTTCACATTCC+TGG - chr1_3:66198635-66198654 Msa0134670:intron 40.0%
GACGAGTGAAGTGCAATTTA+AGG - chr1_3:66198688-66198707 Msa0134670:intron 40.0%
! ACACTTTCCTCTTCCTTTTG+CGG + chr1_3:66198847-66198866 None:intergenic 40.0%
!! CAAAGTCGAACTTTGGTGTT+AGG + chr1_3:66198947-66198966 None:intergenic 40.0%
ACGTCAGATCTAACTAGGAA+AGG - chr1_3:66198974-66198993 Msa0134670:CDS 40.0%
TCAGATCTAACTAGGAAAGG+AGG - chr1_3:66198977-66198996 Msa0134670:CDS 40.0%
CAGATCTAACTAGGAAAGGA+GGG - chr1_3:66198978-66198997 Msa0134670:CDS 40.0%
AGATCTAACTAGGAAAGGAG+GGG - chr1_3:66198979-66198998 Msa0134670:CDS 40.0%
ATTAGACTCGAGTAGAGTCT+TGG + chr1_3:66199219-66199238 None:intergenic 40.0%
! ACTTTAGGAACAAATGCAGG+TGG + chr1_3:66199507-66199526 None:intergenic 40.0%
! CGAACTTTAGGAACAAATGC+AGG + chr1_3:66199510-66199529 None:intergenic 40.0%
!! TTTGAAGCTGGAGCTTTGTT+GGG + chr1_3:66199654-66199673 None:intergenic 40.0%
!!! GGTGGTTTTATTTTGAAGGG+TGG - chr1_3:66199810-66199829 Msa0134670:intron 40.0%
TCCCCAAGTTGTAAATGTGA+AGG - chr1_3:66199843-66199862 Msa0134670:intron 40.0%
CCCTTCACATTTACAACTTG+GGG + chr1_3:66199847-66199866 None:intergenic 40.0%
CCCCAAGTTGTAAATGTGAA+GGG - chr1_3:66199844-66199863 Msa0134670:intron 40.0%
CACCCTTCACATTTACAACT+TGG + chr1_3:66199849-66199868 None:intergenic 40.0%
! GAAGGGTGCGAAAATATACA+TGG - chr1_3:66199861-66199880 Msa0134670:intron 40.0%
GGTGCGAAAATATACATGGT+AGG - chr1_3:66199865-66199884 Msa0134670:intron 40.0%
! CCTCTTGTGAGTTGGAAACA+AGG + chr1_3:66196688-66196707 None:intergenic 45.0%
! CCTTGTTTCCAACTCACAAG+AGG - chr1_3:66196685-66196704 Msa0134670:CDS 45.0%
ACTATGACGCTGAACTCACT+TGG - chr1_3:66196997-66197016 Msa0134670:intron 45.0%
CAAGTACCTCGTGAGGAATA+TGG + chr1_3:66197073-66197092 None:intergenic 45.0%
ACAGCCTCAGATGAAAGTTC+AGG + chr1_3:66197199-66197218 None:intergenic 45.0%
CAAGCAAGAACCAGATGGTA+CGG - chr1_3:66197342-66197361 Msa0134670:intron 45.0%
TCTTGTACATCCGTACCATC+TGG + chr1_3:66197355-66197374 None:intergenic 45.0%
TCTGTCACAGATGCAGTTGA+AGG - chr1_3:66197379-66197398 Msa0134670:intron 45.0%
GGTGGAACATTTATTCGGGA+AGG - chr1_3:66197400-66197419 Msa0134670:intron 45.0%
! ACTGTTGACCCACTTGCTTT+TGG - chr1_3:66197514-66197533 Msa0134670:intron 45.0%
TTTCTGTCTGTTGCGGCTAT+TGG + chr1_3:66197574-66197593 None:intergenic 45.0%
CACTGTTGTTGGCAATTGCT+TGG - chr1_3:66197935-66197954 Msa0134670:CDS 45.0%
CAGTCTATACAAAGTAGGGG+TGG - chr1_3:66198004-66198023 Msa0134670:CDS 45.0%
CTGAGATGACCAGTATGCAA+AGG - chr1_3:66198342-66198361 Msa0134670:intron 45.0%
ATTGGTGCAGAGACTTGAGA+TGG + chr1_3:66198575-66198594 None:intergenic 45.0%
ATCTCAAGTCTCTGCACCAA+TGG - chr1_3:66198574-66198593 Msa0134670:intron 45.0%
AGCCGTAAATGTGTCTCCAT+TGG + chr1_3:66198593-66198612 None:intergenic 45.0%
CTCTGACAGAAATCTTCACC+AGG + chr1_3:66198656-66198675 None:intergenic 45.0%
! TCTGTCAGAGTACTCCCAAA+AGG - chr1_3:66198666-66198685 Msa0134670:intron 45.0%
! TGCACTTCACTCGTCCTTTT+GGG + chr1_3:66198683-66198702 None:intergenic 45.0%
! TTGCACTTCACTCGTCCTTT+TGG + chr1_3:66198684-66198703 None:intergenic 45.0%
! AAAGGACGATGAAGGTGCAT+AGG - chr1_3:66198744-66198763 Msa0134670:intron 45.0%
! ACGATGAAGGTGCATAGGAA+AGG - chr1_3:66198749-66198768 Msa0134670:intron 45.0%
! TCTGTCAGAGTATCCGCAAA+AGG - chr1_3:66198831-66198850 Msa0134670:intron 45.0%
! AGAGTATCCGCAAAAGGAAG+AGG - chr1_3:66198837-66198856 Msa0134670:intron 45.0%
ACACCAAAGTTCGACTTTGC+AGG - chr1_3:66198948-66198967 Msa0134670:intron 45.0%
GTTCCTGCAAAGTCGAACTT+TGG + chr1_3:66198954-66198973 None:intergenic 45.0%
GGAACACGTCAGATCTAACT+AGG - chr1_3:66198969-66198988 Msa0134670:CDS 45.0%
AAGCAGTCTTGACATGAGCA+AGG + chr1_3:66199423-66199442 None:intergenic 45.0%
TGGTTCCTCACCTCAAATTG+TGG - chr1_3:66199539-66199558 Msa0134670:CDS 45.0%
! TCTGACCACAATTTGAGGTG+AGG + chr1_3:66199547-66199566 None:intergenic 45.0%
!! TTGAAGCTGGAGCTTTGTTG+GGG + chr1_3:66199653-66199672 None:intergenic 45.0%
!! CTTTGAAGCTGGAGCTTTGT+TGG + chr1_3:66199655-66199674 None:intergenic 45.0%
!!! TTATTTTATTTTTTTACTTT+TGG - chr1_3:66199582-66199601 Msa0134670:CDS 5.0%
ACGAGTCCATATTCCTCACG+AGG - chr1_3:66197064-66197083 Msa0134670:CDS 50.0%
CTTGGCAAGCAAGAACCAGA+TGG - chr1_3:66197337-66197356 Msa0134670:intron 50.0%
GTCACAGATGCAGTTGAAGG+TGG - chr1_3:66197382-66197401 Msa0134670:intron 50.0%
!! GTTGCGGCTATTGGTTCTGA+AGG + chr1_3:66197565-66197584 None:intergenic 50.0%
! GGTGGGGTGAATTGAGGTTT+TGG - chr1_3:66198070-66198089 Msa0134670:CDS 50.0%
TTGGATCCTACCACTCAAGC+TGG - chr1_3:66198089-66198108 Msa0134670:CDS 50.0%
! TAAGTGCAGCTCGGTGATTC+AGG + chr1_3:66198518-66198537 None:intergenic 50.0%
! AATTGTGGCTGCTTTGCTGC+TGG - chr1_3:66199381-66199400 Msa0134670:CDS 50.0%
ATACAGTTCTGCAAGCTCGC+AGG - chr1_3:66199463-66199482 Msa0134670:intron 50.0%
GCAGAATACGATGCAAGTGC+AGG - chr1_3:66199682-66199701 Msa0134670:intron 50.0%
GACGCTGAACTCACTTGGCA+TGG - chr1_3:66197002-66197021 Msa0134670:intron 55.0%
GGTGAACCAGCTTGAGTGGT+AGG + chr1_3:66198098-66198117 None:intergenic 55.0%
AACGGGTGAACCAGCTTGAG+TGG + chr1_3:66198102-66198121 None:intergenic 55.0%
!! CTGCGTTGTCGCTTTGAAGC+TGG + chr1_3:66199666-66199685 None:intergenic 55.0%
GCTGCTGGAGTCTACTGCAC+AGG - chr1_3:66199396-66199415 Msa0134670:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 66196659 66199965 66196659 ID=Msa0134670;Name=Msa0134670
chr1_3 mRNA 66196659 66199965 66196659 ID=Msa0134670-mRNA-1;Parent=Msa0134670;Name=Msa0134670-mRNA-1;_AED=0.23;_eAED=0.23;_QI=0|0|0|1|1|1|7|0|563
chr1_3 exon 66199918 66199965 66199918 ID=Msa0134670-mRNA-1:exon:22619;Parent=Msa0134670-mRNA-1
chr1_3 exon 66199539 66199699 66199539 ID=Msa0134670-mRNA-1:exon:22618;Parent=Msa0134670-mRNA-1
chr1_3 exon 66198961 66199453 66198961 ID=Msa0134670-mRNA-1:exon:22617;Parent=Msa0134670-mRNA-1
chr1_3 exon 66197760 66198331 66197760 ID=Msa0134670-mRNA-1:exon:22616;Parent=Msa0134670-mRNA-1
chr1_3 exon 66197064 66197244 66197064 ID=Msa0134670-mRNA-1:exon:22615;Parent=Msa0134670-mRNA-1
chr1_3 exon 66196881 66196979 66196881 ID=Msa0134670-mRNA-1:exon:22614;Parent=Msa0134670-mRNA-1
chr1_3 exon 66196659 66196796 66196659 ID=Msa0134670-mRNA-1:exon:22613;Parent=Msa0134670-mRNA-1
chr1_3 CDS 66199918 66199965 66199918 ID=Msa0134670-mRNA-1:cds;Parent=Msa0134670-mRNA-1
chr1_3 CDS 66199539 66199699 66199539 ID=Msa0134670-mRNA-1:cds;Parent=Msa0134670-mRNA-1
chr1_3 CDS 66198961 66199453 66198961 ID=Msa0134670-mRNA-1:cds;Parent=Msa0134670-mRNA-1
chr1_3 CDS 66197760 66198331 66197760 ID=Msa0134670-mRNA-1:cds;Parent=Msa0134670-mRNA-1
chr1_3 CDS 66197064 66197244 66197064 ID=Msa0134670-mRNA-1:cds;Parent=Msa0134670-mRNA-1
chr1_3 CDS 66196881 66196979 66196881 ID=Msa0134670-mRNA-1:cds;Parent=Msa0134670-mRNA-1
chr1_3 CDS 66196659 66196796 66196659 ID=Msa0134670-mRNA-1:cds;Parent=Msa0134670-mRNA-1
Gene Sequence

>Msa0134670

ATGGCGGCACTAGATCACAACAACACCCTTGTTTCCAACTCACAAGAGGATGATTCTTTCTTTGACTACCTCAATACTCTATCTCCTTTAAACACCAAAAAGTATGTTGGAGTTGGAGCTACTATGACGCTGAACTCACTTGGCATGGCTTATCCTCTTTTCACATCACTCGATGTGACCTTTAATGACGAGTCCATATTCCTCACGAGGAATAATCTTTTGTACACATCAAATCCTGAACTTTCATCTGAGGCTGTAAATAACACATATTCACATGAAGAAGCTCCTACAGATTCAACTCATGCATGGATTGACACAAGTCAGCTACATGATCCTCAAATCTCAAGTGATGATGCCAAGTATCTTCTTGAATTACTTGGCAAGCAAGAACCAGATGGTACGGATGTACAAGATGATTCTGTCACAGATGCAGTTGAAGGTGGAACATTTATTCGGGAAGGACCAGATTGTGATTACAATAACTTAATTAGTGCAACACAATCTGTGACCATGTTACCACAATCTACCAGTAATAGTTATTATAAAATGCAAACTGTTGACCCACTTGCTTTTGGTTCAAAACATGAAATTGAAGACAATCCTTCAGAACCAATAGCCGCAACAGACAGAAATCAAGCACAGGACAATCTTGCCAATGTTGCTTTAATGGATATCAATCAAATTAAGAGAGGGGATGATGAGCTTGCACGTATTAGACGCAGTATACAGATGAGCTGTCAAGGCTATGAGTCTGAGATGACCAGTATGCAAAGGAACAACTCTGATGAGAGGAATGCTATTAACAAAAACCCTAGTGATTCACAGAAGTGTGTTTTGCAAGGAAATGGTTTTTACTGGAATGCACTTCCAGCCTTAAATCATTACGAAGGCATAGAAAATGAAAAGATGTCTTCTCAAAGGAAACCTGAATCACCGAGCTGCACTTATTCTTTGAATATTTCTGTAAGTCAAGGGTATCAACCATCTCAAGTCTCTGCACCAATGGAGACACATTTACGGCTATCTGAGAATGAAGATGTCACCAAGTCATCAGTTCACATTCCTGGTGAAGATTTCTGTCAGAGTACTCCCAAAAGGACGAGTGAAGTGCAATTTAAGGACATAAAGAACAAAAAGTTAGATCAAAACATGAAAAGGACGATGAAGGTGCATAGGAAAGGCTCGATTGATCTAGTTTCTTCTGGTTCAGAACATGTAAAGGAAGAGGAAAGAGCTGATTTCTGTCAGAGTATCCGCAAAAGGAAGAGGAAAGTTAATTGTGGCTGCTTTGCTGCTGGAGTCTACTGCACAGGACCTTGCTCATGTCAAGACTGCTTGAATAAACATATTAATGAAGATACAGTTCTGCAAGCTCGCAGGATGATTGAGTATCGTAATCCACCTGCATTTGTTCCTAAAGTTCGAAAGAATTCTGGTTCCTCACCTCAAATTGTGGATGACCCCAACAAAGCTCCAGCTTCAAAGCGACAACGCAGAATACGATGCAAGTGCAGGAAATCAAGCTGCATGAATAAACGTTGTGAATGTTTTAAGGGTGGTGTTGCTTGTTCCCCAAGTTGTAAATGTGAAGGGTGCGAAAATATACATGGTAGGAAGGATAGTGCAGCTGAAACAAAATCTGAGCTTGAAGAAACAGAAGCATCACAGATAAAGCTACTAATTGGTATGTAA
Protein sequence

>Msa0134670

MAALDHNNTLVSNSQEDDSFFDYLNTLSPLNTKKYVGVGATMTLNSLGMAYPLFTSLDVTFNDESIFLTRNNLLYTSNPELSSEAVNNTYSHEEAPTDSTHAWIDTSQLHDPQISSDDAKYLLELLGKQEPDGTDVQDDSVTDAVEGGTFIREGPDCDYNNLISATQSVTMLPQSTSNSYYKMQTVDPLAFGSKHEIEDNPSEPIAATDRNQAQDNLANVALMDINQIKRGDDELARIRRSIQMSCQGYESEMTSMQRNNSDERNAINKNPSDSQKCVLQGNGFYWNALPALNHYEGIENEKMSSQRKPESPSCTYSLNISVSQGYQPSQVSAPMETHLRLSENEDVTKSSVHIPGEDFCQSTPKRTSEVQFKDIKNKKLDQNMKRTMKVHRKGSIDLVSSGSEHVKEEERADFCQSIRKRKRKVNCGCFAAGVYCTGPCSCQDCLNKHINEDTVLQARRMIEYRNPPAFVPKVRKNSGSSPQIVDDPNKAPASKRQRRIRCKCRKSSCMNKRCECFKGGVACSPSCKCEGCENIHGRKDSAAETKSELEETEASQIKLLIGM*