AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0135800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0135800 MtrunA17_Chr1g0206721 93.846 130 8 0 1 130 1 130 1.16e-85 249
Msa0135800 MtrunA17_Chr1g0206701 93.846 130 8 0 1 130 1 130 2.84e-85 248
Msa0135800 MtrunA17_Chr3g0099411 95.238 126 6 0 1 126 1 126 2.15e-84 245
Msa0135800 MtrunA17_Chr8g0355091 93.077 130 9 0 1 130 1 130 3.68e-84 246
Msa0135800 MtrunA17_Chr1g0149391 91.473 129 11 0 1 129 1 129 4.61e-83 243
Msa0135800 MtrunA17_Chr3g0099421 88.462 130 15 0 1 130 26 155 1.39e-80 237
Msa0135800 MtrunA17_Chr1g0206991 88.462 130 15 0 1 130 1 130 4.73e-80 233
Msa0135800 MtrunA17_Chr2g0292791 87.692 130 16 0 1 130 1 130 2.46e-78 231
Msa0135800 MtrunA17_Chr1g0208561 80.769 130 25 0 1 130 1 130 1.08e-72 216
Msa0135800 MtrunA17_Chr3g0089141 80.769 130 25 0 1 130 1 130 3.18e-72 215
Msa0135800 MtrunA17_Chr1g0206691 90.265 113 11 0 18 130 1 113 7.38e-71 211
Msa0135800 MtrunA17_Chr2g0300421 77.344 128 29 0 1 128 1 128 1.66e-69 206
Msa0135800 MtrunA17_Chr1g0208581 77.692 130 29 0 1 130 1 130 1.04e-68 206
Msa0135800 MtrunA17_Chr2g0325971 77.692 130 29 0 1 130 1 130 8.81e-68 204
Msa0135800 MtrunA17_Chr3g0089151 73.643 129 34 0 1 129 1 129 2.43e-66 197
Msa0135800 MtrunA17_Chr5g0419601 75.000 128 32 0 1 128 1 128 1.31e-65 199
Msa0135800 MtrunA17_Chr1g0187361 75.000 128 32 0 1 128 1 128 8.83e-65 196
Msa0135800 MtrunA17_Chr1g0188671 72.656 128 35 0 1 128 39 166 4.26e-64 196
Msa0135800 MtrunA17_Chr2g0300441 75.194 129 32 0 1 129 1 129 1.15e-62 191
Msa0135800 MtrunA17_Chr2g0317481 70.312 128 38 0 1 128 1 128 3.73e-62 190
Msa0135800 MtrunA17_Chr2g0317451 70.312 128 38 0 1 128 1 128 3.77e-62 190
Msa0135800 MtrunA17_Chr5g0422941 69.291 127 39 0 1 127 1 127 1.41e-60 186
Msa0135800 MtrunA17_Chr5g0422421 67.969 128 41 0 1 128 1 128 1.90e-56 175
Msa0135800 MtrunA17_Chr3g0085241 61.417 127 49 0 3 129 35 161 4.86e-54 169
Msa0135800 MtrunA17_Chr3g0119901 63.415 123 45 0 7 129 7 129 2.03e-53 167
Msa0135800 MtrunA17_Chr3g0111931 66.019 103 35 0 21 123 2 104 1.29e-46 146
Msa0135800 MtrunA17_Chr7g0238991 67.890 109 34 1 16 123 27 135 3.58e-45 147
Msa0135800 MtrunA17_Chr1g0187351 80.303 66 13 0 18 83 1 66 7.39e-35 115
Msa0135800 MtrunA17_Chr2g0333981 43.307 127 62 2 5 128 8 127 6.20e-33 114
Msa0135800 MtrunA17_Chr8g0379021 42.520 127 63 2 5 128 8 127 2.12e-31 110
Msa0135800 MtrunA17_Chr3g0111841 83.051 59 10 0 15 73 2 60 4.01e-31 105
Msa0135800 MtrunA17_Chr4g0018951 47.200 125 58 2 5 129 8 124 3.17e-30 106
Msa0135800 MtrunA17_Chr4g0071601 43.307 127 62 2 5 128 8 127 3.12e-26 97.1
Msa0135800 MtrunA17_Chr3g0111831 70.492 61 18 0 16 76 11 71 1.66e-25 92.0
Msa0135800 MtrunA17_Chr1g0175601 44.144 111 59 2 9 118 2 110 2.89e-25 94.4
Msa0135800 MtrunA17_Chr1g0169761 39.552 134 68 3 9 129 2 135 3.95e-24 91.3
Msa0135800 MtrunA17_Chr8g0376821 42.857 112 59 3 9 118 2 110 1.77e-23 89.7
Msa0135800 MtrunA17_Chr4g0074101 38.095 126 62 4 9 118 2 127 1.97e-23 90.1
Msa0135800 MtrunA17_Chr8g0357771 40.800 125 69 2 9 130 2 124 2.22e-23 89.4
Msa0135800 MtrunA17_Chr1g0180591 43.750 112 58 3 9 118 2 110 2.60e-23 89.4
Msa0135800 MtrunA17_Chr1g0175611 41.593 113 64 1 9 121 2 112 7.62e-23 87.8
Msa0135800 MtrunA17_Chr8g0356071 37.302 126 70 2 9 130 2 122 6.34e-22 85.5
Msa0135800 MtrunA17_Chr4g0052061 39.623 106 62 1 9 114 2 105 6.69e-21 82.4
Msa0135800 MtrunA17_Chr4g0052081 39.623 106 63 1 9 113 2 107 3.09e-19 78.6
Msa0135800 MtrunA17_Chr3g0077501 50.000 68 34 0 9 76 2 69 9.26e-18 75.9
Msa0135800 MtrunA17_Chr4g0057151 47.945 73 38 0 10 82 3 75 2.08e-17 74.7
Msa0135800 MtrunA17_Chr3g0097101 40.566 106 53 1 3 108 11 106 2.93e-17 75.1
Msa0135800 MtrunA17_Chr4g0049901 53.030 66 31 0 10 75 29 94 3.46e-17 74.7
Msa0135800 MtrunA17_Chr8g0351041 44.872 78 43 0 10 87 3 80 1.08e-16 71.6
Msa0135800 MtrunA17_Chr5g0427401 48.529 68 35 0 9 76 2 69 1.66e-16 72.4
Msa0135800 MtrunA17_Chr2g0284911 38.182 110 67 1 9 118 17 125 1.98e-16 72.8
Msa0135800 MtrunA17_Chr8g0351051 48.529 68 35 0 9 76 2 69 2.76e-16 72.4
Msa0135800 MtrunA17_Chr6g0474371 46.377 69 36 1 9 76 2 70 5.09e-16 70.9
Msa0135800 MtrunA17_Chr8g0351031 44.872 78 43 0 10 87 3 80 6.11e-16 71.2
Msa0135800 MtrunA17_Chr1g0204111 47.059 68 36 0 9 76 18 85 9.84e-16 70.9
Msa0135800 MtrunA17_Chr8g0380021 48.529 68 35 0 9 76 18 85 1.10e-15 70.9
Msa0135800 MtrunA17_Chr4g0061691 44.928 69 37 1 9 76 2 70 1.24e-15 70.5
Msa0135800 MtrunA17_Chr7g0246431 36.364 110 68 1 10 119 3 110 1.25e-15 70.1
Msa0135800 MtrunA17_Chr2g0279821 45.588 68 37 0 9 76 2 69 1.30e-15 70.5
Msa0135800 MtrunA17_Chr4g0049541 44.118 68 38 0 9 76 2 69 3.81e-15 68.9
Msa0135800 MtrunA17_Chr5g0404951 47.143 70 35 1 9 76 2 71 6.52e-15 68.6
Msa0135800 MtrunA17_Chr3g0134451 39.423 104 60 3 9 111 2 103 7.56e-15 69.3
Msa0135800 MtrunA17_Chr6g0455511 46.377 69 36 1 9 76 2 70 7.60e-15 68.6
Msa0135800 MtrunA17_Chr7g0220111 46.377 69 36 1 9 76 2 70 1.28e-14 67.8
Msa0135800 MtrunA17_Chr4g0020221 32.877 146 67 2 9 123 2 147 1.40e-14 67.8
Msa0135800 MtrunA17_Chr5g0411691 45.588 68 37 0 9 76 2 69 2.28e-14 67.0
Msa0135800 MtrunA17_Chr1g0173161 53.571 56 26 0 9 64 2 57 2.33e-14 63.5
Msa0135800 MtrunA17_Chr8g0366231 43.478 69 38 1 9 76 2 70 2.45e-14 67.0
Msa0135800 MtrunA17_Chr5g0412271 44.118 68 38 0 9 76 2 69 2.57e-14 66.6
Msa0135800 MtrunA17_Chr5g0412461 44.118 68 38 0 9 76 2 69 3.22e-14 66.6
Msa0135800 MtrunA17_Chr7g0220121 40.580 69 40 1 9 76 2 70 3.45e-14 67.0
Msa0135800 MtrunA17_Chr5g0420081 36.170 94 48 3 9 90 2 95 4.56e-14 66.2
Msa0135800 MtrunA17_Chr2g0308251 43.056 72 41 0 9 80 2 73 5.78e-14 62.8
Msa0135800 MtrunA17_Chr5g0420091 44.928 69 37 1 9 76 2 70 7.66e-14 65.5
Msa0135800 MtrunA17_Chr3g0125241 41.429 70 39 1 9 76 27 96 1.07e-13 64.7
Msa0135800 MtrunA17_Chr1g0159661 43.939 66 36 1 9 74 2 66 1.17e-13 64.3
Msa0135800 MtrunA17_Chr1g0164791 44.286 70 37 1 9 76 2 71 1.17e-13 65.1
Msa0135800 MtrunA17_Chr5g0427921 45.614 57 31 0 9 65 2 58 3.70e-13 60.1
Msa0135800 MtrunA17_Chr4g0061671 47.368 57 30 0 9 65 2 58 3.84e-13 63.2
Msa0135800 MtrunA17_Chr8g0377561 50.000 56 28 0 9 64 2 57 4.31e-13 60.8
Msa0135800 MtrunA17_Chr3g0142191 42.857 70 38 1 9 76 2 71 4.99e-13 63.5
Msa0135800 MtrunA17_Chr7g0246381 44.776 67 32 1 9 70 2 68 5.10e-13 60.1
Msa0135800 MtrunA17_Chr3g0122251 51.786 56 27 0 9 64 2 57 6.05e-13 63.5
Msa0135800 MtrunA17_Chr5g0425191 50.943 53 26 0 9 61 2 54 1.32e-12 59.7
Msa0135800 MtrunA17_Chr4g0044071 47.368 57 30 0 9 65 2 58 1.95e-12 59.7
Msa0135800 MtrunA17_Chr8g0345001 40.000 75 45 0 8 82 17 91 2.70e-12 60.8
Msa0135800 MtrunA17_Chr4g0025531 40.741 81 48 0 1 81 1 81 3.63e-11 58.5
Msa0135800 MtrunA17_Chr3g0093131 48.148 54 27 1 26 78 36 89 5.22e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0135800 AT5G60440.1 62.500 128 46 1 5 130 3 130 5.08e-51 164
Msa0135800 AT4G36590.1 51.613 124 53 2 5 123 3 124 1.68e-35 122
Msa0135800 AT2G24840.1 50.000 128 56 3 6 130 60 182 1.08e-34 121
Msa0135800 AT1G01530.1 54.717 106 46 1 4 109 2 105 5.99e-34 119
Msa0135800 AT1G65360.1 48.246 114 57 1 5 118 3 114 1.52e-32 114
Msa0135800 AT2G34440.1 43.590 117 57 2 9 121 2 113 2.82e-27 99.8
Msa0135800 AT3G66656.1 42.017 119 65 1 9 123 2 120 2.69e-25 94.7
Msa0135800 AT1G72350.1 53.425 73 32 1 9 81 43 113 1.42e-21 86.3
Msa0135800 AT3G04100.1 33.913 115 73 1 2 113 7 121 2.04e-20 82.8
Msa0135800 AT4G22950.2 47.368 76 40 0 10 85 3 78 1.07e-18 77.4
Msa0135800 AT2G45660.2 41.748 103 53 3 10 106 3 104 4.22e-18 75.9
Msa0135800 AT4G22950.3 49.296 71 36 0 10 80 3 73 7.85e-18 76.3
Msa0135800 AT4G22950.1 49.296 71 36 0 10 80 3 73 7.85e-18 76.3
Msa0135800 AT2G45660.1 50.000 68 34 0 10 77 3 70 1.12e-17 75.9
Msa0135800 AT2G45650.1 50.000 68 34 0 9 76 2 69 1.13e-17 76.6
Msa0135800 AT4G09960.2 44.186 86 47 1 9 93 2 87 1.73e-17 75.5
Msa0135800 AT4G09960.1 44.186 86 47 1 9 93 2 87 1.98e-17 75.5
Msa0135800 AT4G09960.6 44.186 86 47 1 9 93 3 88 2.12e-17 75.5
Msa0135800 AT4G09960.5 44.186 86 47 1 9 93 12 97 2.94e-17 75.1
Msa0135800 AT4G09960.3 44.186 86 47 1 9 93 28 113 3.34e-17 75.5
Msa0135800 AT4G09960.4 44.186 86 47 1 9 93 97 182 7.34e-17 75.1
Msa0135800 AT5G51870.1 50.746 67 33 0 10 76 3 69 1.15e-16 73.2
Msa0135800 AT5G51870.2 50.746 67 33 0 10 76 3 69 1.51e-16 72.0
Msa0135800 AT5G51870.4 50.746 67 33 0 10 76 3 69 2.11e-16 72.4
Msa0135800 AT1G28460.1 34.694 98 64 0 5 102 4 101 2.70e-16 71.6
Msa0135800 AT5G51870.3 50.746 67 33 0 10 76 13 79 2.82e-16 72.4
Msa0135800 AT4G18960.1 38.318 107 56 1 2 108 11 107 2.95e-16 72.8
Msa0135800 AT5G51870.5 47.222 72 38 0 5 76 21 92 3.59e-16 71.6
Msa0135800 AT5G62165.14 46.269 67 36 0 10 76 3 69 4.37e-16 69.3
Msa0135800 AT3G57230.2 44.118 68 38 0 9 76 2 69 4.46e-16 70.1
Msa0135800 AT3G61120.1 48.529 68 35 0 9 76 2 69 5.61e-16 72.0
Msa0135800 AT5G62165.17 46.269 67 36 0 10 76 3 69 5.91e-16 70.1
Msa0135800 AT5G62165.12 46.269 67 36 0 10 76 3 69 5.91e-16 70.1
Msa0135800 AT5G62165.11 46.269 67 36 0 10 76 3 69 5.91e-16 70.1
Msa0135800 AT5G23260.1 44.681 94 44 3 5 90 12 105 6.47e-16 72.0
Msa0135800 AT5G23260.4 44.681 94 44 3 5 90 12 105 7.06e-16 72.0
Msa0135800 AT5G62165.6 46.269 67 36 0 10 76 3 69 8.41e-16 69.7
Msa0135800 AT5G62165.9 46.269 67 36 0 10 76 3 69 8.41e-16 69.7
Msa0135800 AT5G62165.7 46.269 67 36 0 10 76 3 69 8.41e-16 69.7
Msa0135800 AT5G62165.8 46.269 67 36 0 10 76 3 69 8.41e-16 69.7
Msa0135800 AT5G62165.13 46.269 67 36 0 10 76 3 69 8.41e-16 69.7
Msa0135800 AT4G37940.4 45.588 68 37 0 9 76 2 69 8.54e-16 70.9
Msa0135800 AT4G37940.1 45.588 68 37 0 9 76 2 69 8.84e-16 71.2
Msa0135800 AT2G42830.1 48.529 68 35 0 9 76 17 84 1.11e-15 71.2
Msa0135800 AT5G23260.2 44.318 88 43 2 9 90 2 89 1.16e-15 71.2
Msa0135800 AT2G42830.2 48.529 68 35 0 9 76 17 84 1.17e-15 71.2
Msa0135800 AT4G37940.3 45.588 68 37 0 9 76 2 69 1.22e-15 69.7
Msa0135800 AT5G13790.2 40.860 93 46 1 9 101 2 85 1.26e-15 71.2
Msa0135800 AT5G23260.3 44.318 88 43 2 9 90 2 89 1.31e-15 70.9
Msa0135800 AT3G58780.1 47.887 71 37 0 6 76 14 84 1.47e-15 70.9
Msa0135800 AT3G58780.5 47.887 71 37 0 6 76 20 90 1.68e-15 70.9
Msa0135800 AT5G10140.2 47.826 69 35 1 9 76 2 70 1.69e-15 69.3
Msa0135800 AT5G51860.2 49.254 67 34 0 10 76 3 69 1.70e-15 69.7
Msa0135800 AT4G11880.2 45.833 72 38 1 10 80 3 74 1.95e-15 69.3
Msa0135800 AT4G11880.3 45.833 72 38 1 10 80 3 74 1.95e-15 69.3
Msa0135800 AT3G57390.3 47.059 68 36 0 9 76 2 69 2.18e-15 70.1
Msa0135800 AT3G57230.1 44.118 68 38 0 9 76 2 69 2.18e-15 70.1
Msa0135800 AT3G57230.4 44.118 68 38 0 9 76 2 69 2.18e-15 70.1
Msa0135800 AT4G24540.1 47.059 68 36 0 9 76 2 69 2.21e-15 69.7
Msa0135800 AT5G62165.10 46.269 67 36 0 10 76 3 69 2.24e-15 69.3
Msa0135800 AT5G62165.4 46.269 67 36 0 10 76 3 69 2.24e-15 69.3
Msa0135800 AT5G62165.3 46.269 67 36 0 10 76 3 69 2.31e-15 69.7
Msa0135800 AT5G62165.1 46.269 67 36 0 10 76 3 69 2.31e-15 69.7
Msa0135800 AT5G62165.2 46.269 67 36 0 10 76 3 69 2.31e-15 69.7
Msa0135800 AT5G13790.1 48.529 68 35 0 9 76 2 69 2.37e-15 70.5
Msa0135800 AT5G51860.1 49.254 67 34 0 10 76 3 69 2.48e-15 69.7
Msa0135800 AT5G10140.4 47.826 69 35 1 9 76 2 70 2.61e-15 68.9
Msa0135800 AT2G22540.3 47.059 68 36 0 9 76 2 69 2.89e-15 70.1
Msa0135800 AT2G22540.1 47.059 68 36 0 9 76 2 69 2.89e-15 70.1
Msa0135800 AT3G58780.4 47.887 71 37 0 6 76 14 84 2.96e-15 70.5
Msa0135800 AT5G10140.3 47.826 69 35 1 9 76 2 70 2.96e-15 68.9
Msa0135800 AT5G10140.1 47.826 69 35 1 9 76 2 70 3.49e-15 68.9
Msa0135800 AT4G11880.4 45.833 72 38 1 10 80 3 74 3.74e-15 69.3
Msa0135800 AT4G11880.1 45.833 72 38 1 10 80 3 74 3.74e-15 69.3
Msa0135800 AT3G54340.1 44.286 70 37 1 9 76 2 71 3.94e-15 69.3
Msa0135800 AT3G57390.1 47.059 68 36 0 9 76 2 69 4.93e-15 69.3
Msa0135800 AT1G29962.1 34.653 101 63 2 5 102 4 104 7.52e-15 67.8
Msa0135800 AT1G77950.3 43.421 76 41 2 9 83 2 76 7.88e-15 68.9
Msa0135800 AT1G77950.2 43.421 76 41 2 9 83 2 76 7.88e-15 68.9
Msa0135800 AT1G77950.1 43.421 76 41 2 9 83 2 76 7.88e-15 68.9
Msa0135800 AT1G77950.4 43.421 76 41 2 9 83 2 76 7.88e-15 68.9
Msa0135800 AT1G22130.1 34.286 105 68 1 9 112 2 106 8.48e-15 69.7
Msa0135800 AT2G22630.1 44.118 68 38 0 9 76 2 69 9.27e-15 68.2
Msa0135800 AT2G22630.2 44.118 68 38 0 9 76 2 69 9.27e-15 68.2
Msa0135800 AT5G60910.1 46.479 71 35 1 9 79 2 69 9.54e-15 68.6
Msa0135800 AT1G69120.2 38.462 91 53 1 9 99 2 89 9.66e-15 68.6
Msa0135800 AT1G77980.1 35.238 105 67 1 9 112 2 106 9.90e-15 69.3
Msa0135800 AT3G30260.1 43.478 69 38 1 9 76 2 70 1.20e-14 68.6
Msa0135800 AT1G26310.1 37.500 96 46 2 9 90 2 97 1.56e-14 68.2
Msa0135800 AT1G69120.1 38.462 91 53 1 9 99 2 89 2.06e-14 67.8
Msa0135800 AT1G17310.1 51.351 74 34 1 8 81 47 118 2.17e-14 67.4
Msa0135800 AT1G17310.2 49.367 79 38 1 8 86 58 134 2.55e-14 67.0
Msa0135800 AT5G15800.1 46.377 69 36 1 9 76 2 70 4.04e-14 67.0
Msa0135800 AT5G20240.2 44.286 70 37 1 9 76 2 71 5.00e-14 65.1
Msa0135800 AT5G20240.1 44.286 70 37 1 9 76 2 71 5.98e-14 65.9
Msa0135800 AT2G14210.1 43.478 69 38 1 9 76 2 70 8.05e-14 65.9
Msa0135800 AT5G15800.2 46.377 69 36 1 9 76 2 70 8.26e-14 66.2
Msa0135800 AT2G14210.2 43.478 69 38 1 9 76 2 70 1.11e-13 65.5
Msa0135800 AT2G03710.3 44.286 70 37 2 9 76 2 71 1.70e-13 64.3
Msa0135800 AT5G65050.2 43.478 69 38 1 9 76 2 70 1.80e-13 63.9
Msa0135800 AT3G02310.2 44.928 69 37 1 9 76 2 70 1.86e-13 64.3
Msa0135800 AT5G65050.5 43.478 69 38 1 9 76 2 70 3.85e-13 63.2
Msa0135800 AT3G58780.2 50.000 60 30 0 6 65 14 73 3.92e-13 64.3
Msa0135800 AT5G65050.3 43.478 69 38 1 9 76 2 70 3.99e-13 63.5
Msa0135800 AT2G22540.2 51.786 56 27 0 9 64 2 57 4.19e-13 63.9
Msa0135800 AT5G65050.1 43.478 69 38 1 9 76 2 70 4.38e-13 63.2
Msa0135800 AT1G47760.1 31.405 121 67 3 9 129 2 106 4.92e-13 63.2
Msa0135800 AT3G58780.3 50.000 60 30 0 6 65 14 73 5.05e-13 64.3
Msa0135800 AT2G03710.2 44.286 70 37 2 9 76 2 71 6.69e-13 63.9
Msa0135800 AT2G03710.1 44.286 70 37 2 9 76 2 71 6.89e-13 63.5
Msa0135800 AT5G65060.3 43.478 69 38 1 9 76 2 70 7.61e-13 62.8
Msa0135800 AT1G24260.1 50.000 56 28 0 9 64 2 57 7.77e-13 63.5
Msa0135800 AT1G24260.2 50.000 56 28 0 9 64 2 57 8.00e-13 63.5
Msa0135800 AT1G24260.3 50.000 56 28 0 9 64 2 57 9.64e-13 63.2
Msa0135800 AT5G65060.2 43.478 69 38 1 9 76 2 70 9.78e-13 62.4
Msa0135800 AT5G65060.1 43.478 69 38 1 9 76 2 70 1.10e-12 62.4
Msa0135800 AT3G02310.1 44.928 69 37 1 9 76 2 70 1.29e-12 62.8
Msa0135800 AT1G71692.1 42.029 69 39 1 10 77 3 71 1.41e-12 62.4
Msa0135800 AT5G65070.4 39.130 69 41 1 9 76 2 70 6.23e-12 59.3
Msa0135800 AT5G65070.1 39.130 69 41 1 9 76 2 70 1.02e-11 59.7
Msa0135800 AT1G28450.1 30.693 101 67 2 5 102 4 104 1.40e-11 59.3
Msa0135800 AT5G65070.2 39.130 69 41 1 9 76 2 70 1.97e-11 59.3
Msa0135800 AT5G65070.5 39.130 69 41 1 9 76 2 70 3.05e-11 58.5
Msa0135800 AT4G37435.1 37.500 72 44 1 9 79 2 73 3.99e-11 58.2

Find 22 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGCATGGTACCATTGTTT+TGG 0.337688 1_3:+67530729 None:intergenic
TCTTCTCGTTGTCTAGTAGC+TGG 0.435079 1_3:+67530645 None:intergenic
ATCTATGACCGTATCAACAT+TGG 0.506900 1_3:+67530775 None:intergenic
GCTCGCGCACGTTGGCGCTA+CGG 0.534754 1_3:+67530693 None:intergenic
TCTATGACCGTATCAACATT+GGG 0.542196 1_3:+67530776 None:intergenic
TGGCCAACCCAATGTTGATA+CGG 0.545005 1_3:-67530783 Msa0135800:CDS
AGCGACATGTGCACCACAGA+TGG 0.558797 1_3:+67530844 None:intergenic
CTTCAACCATGTCGAGTGGA+AGG 0.562158 1_3:-67530982 None:intergenic
AATGAAAATACCTTCTCTCC+AGG 0.563686 1_3:+67530806 None:intergenic
ACTTTCTCGAAGTGCCGTAG+TGG 0.572403 1_3:-67530896 Msa0135800:CDS
CTTTCTCGAAGTGCCGTAGT+GGG 0.579729 1_3:-67530895 Msa0135800:CDS
AGTATTGAGCTCGCGCACGT+TGG 0.584782 1_3:+67530685 None:intergenic
ACAGATGGTACAAAGCTCAC+TGG 0.589892 1_3:+67530859 None:intergenic
TGACCGTATCAACATTGGGT+TGG 0.600758 1_3:+67530780 None:intergenic
TGTGCTTCAACCATGTCGAG+TGG 0.638522 1_3:-67530986 None:intergenic
TGGTACCATGCAATTCATTG+AGG 0.638950 1_3:-67530720 Msa0135800:CDS
GTGAGCCTCAATGAATTGCA+TGG 0.640763 1_3:+67530715 None:intergenic
CGAGTCCCACCCCAAAACAA+TGG 0.641950 1_3:-67530740 Msa0135800:CDS
CTTTCTTGAAGAGCCCACTA+CGG 0.647437 1_3:+67530882 None:intergenic
AGTGAGCTTTGTACCATCTG+TGG 0.652271 1_3:-67530857 Msa0135800:CDS
TCGAGTGGAAGGAAAAGCCG+AGG 0.691796 1_3:-67530971 Msa0135800:CDS
AGACAACGAGAAGAAACGTG+CGG 0.746947 1_3:-67530636 Msa0135800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTCATCTCAATCTTT+TGG + chr1_3:67530636-67530655 None:intergenic 20.0%
AATGAAAATACCTTCTCTCC+AGG + chr1_3:67530775-67530794 None:intergenic 35.0%
!!! GGAGAGAAGGTATTTTCATT+TGG - chr1_3:67530775-67530794 Msa0135800:CDS 35.0%
TCTATGACCGTATCAACATT+GGG + chr1_3:67530805-67530824 None:intergenic 35.0%
ATCTATGACCGTATCAACAT+TGG + chr1_3:67530806-67530825 None:intergenic 35.0%
! TTGCATGGTACCATTGTTTT+GGG + chr1_3:67530851-67530870 None:intergenic 35.0%
! ATTGCATGGTACCATTGTTT+TGG + chr1_3:67530852-67530871 None:intergenic 35.0%
!! TGCATGGTACCATTGTTTTG+GGG + chr1_3:67530850-67530869 None:intergenic 40.0%
TGGTACCATGCAATTCATTG+AGG - chr1_3:67530858-67530877 Msa0135800:CDS 40.0%
! TCTCAATCTTTTGGCGACCT+CGG + chr1_3:67530627-67530646 None:intergenic 45.0%
CTTTCTTGAAGAGCCCACTA+CGG + chr1_3:67530699-67530718 None:intergenic 45.0%
ACAGATGGTACAAAGCTCAC+TGG + chr1_3:67530722-67530741 None:intergenic 45.0%
! AGTGAGCTTTGTACCATCTG+TGG - chr1_3:67530721-67530740 Msa0135800:CDS 45.0%
! TGTTTTCTCACCTGGAGAGA+AGG - chr1_3:67530762-67530781 Msa0135800:CDS 45.0%
TGGCCAACCCAATGTTGATA+CGG - chr1_3:67530795-67530814 Msa0135800:CDS 45.0%
TGACCGTATCAACATTGGGT+TGG + chr1_3:67530801-67530820 None:intergenic 45.0%
!!! TGGTACCATTGTTTTGGGGT+GGG + chr1_3:67530846-67530865 None:intergenic 45.0%
!!! ATGGTACCATTGTTTTGGGG+TGG + chr1_3:67530847-67530866 None:intergenic 45.0%
GTGAGCCTCAATGAATTGCA+TGG + chr1_3:67530866-67530885 None:intergenic 45.0%
TCTTCTCGTTGTCTAGTAGC+TGG + chr1_3:67530936-67530955 None:intergenic 45.0%
AGACAACGAGAAGAAACGTG+CGG - chr1_3:67530942-67530961 Msa0135800:CDS 45.0%
!! ACTTTCTCGAAGTGCCGTAG+TGG - chr1_3:67530682-67530701 Msa0135800:CDS 50.0%
!! CTTTCTCGAAGTGCCGTAGT+GGG - chr1_3:67530683-67530702 Msa0135800:CDS 50.0%
!! GCTCTCGTTGTTTTCTCACC+TGG - chr1_3:67530754-67530773 Msa0135800:CDS 50.0%
TCGAGTGGAAGGAAAAGCCG+AGG - chr1_3:67530607-67530626 Msa0135800:CDS 55.0%
AGCGACATGTGCACCACAGA+TGG + chr1_3:67530737-67530756 None:intergenic 55.0%
CGAGTCCCACCCCAAAACAA+TGG - chr1_3:67530838-67530857 Msa0135800:CDS 55.0%
! AGTATTGAGCTCGCGCACGT+TGG + chr1_3:67530896-67530915 None:intergenic 55.0%
GCTCGCGCACGTTGGCGCTA+CGG + chr1_3:67530888-67530907 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 67530604 67530996 67530604 ID=Msa0135800;Name=Msa0135800
chr1_3 mRNA 67530604 67530996 67530604 ID=Msa0135800-mRNA-1;Parent=Msa0135800;Name=Msa0135800-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0|-1|0|1|-1|1|1|0|130
chr1_3 exon 67530604 67530996 67530604 ID=Msa0135800-mRNA-1:exon:23175;Parent=Msa0135800-mRNA-1
chr1_3 CDS 67530604 67530996 67530604 ID=Msa0135800-mRNA-1:cds;Parent=Msa0135800-mRNA-1
Gene Sequence

>Msa0135800

ATGTCGAGTGGAAGGAAAAGCCGAGGTCGCCAAAAGATTGAGATGAAAAAAATGAGCAACGAGAGCAACTTGCAAGTGACTTTCTCGAAGTGCCGTAGTGGGCTCTTCAAGAAAGCCAGTGAGCTTTGTACCATCTGTGGTGCACATGTCGCTCTCGTTGTTTTCTCACCTGGAGAGAAGGTATTTTCATTTGGCCAACCCAATGTTGATACGGTCATAGATCGTTATCTTTCTCGAGTCCCACCCCAAAACAATGGTACCATGCAATTCATTGAGGCTCACCGTAGCGCCAACGTGCGCGAGCTCAATACTCAGTTGACTCAGATCAACCAGCTACTAGACAACGAGAAGAAACGTGCGGAAGAGCTGAGACACTTGCGCAAAGCTACATAG
Protein sequence

>Msa0135800

MSSGRKSRGRQKIEMKKMSNESNLQVTFSKCRSGLFKKASELCTICGAHVALVVFSPGEKVFSFGQPNVDTVIDRYLSRVPPQNNGTMQFIEAHRSANVRELNTQLTQINQLLDNEKKRAEELRHLRKAT*