AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0136910

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0136910 MtrunA17_Chr1g0208771 94.000 450 19 1 35 476 12 461 0.0 887
Msa0136910 MtrunA17_Chr8g0390841 65.408 503 138 6 1 474 24 519 0.0 663
Msa0136910 MtrunA17_Chr1g0163071 51.163 301 131 7 36 324 2 298 2.47e-91 280
Msa0136910 MtrunA17_Chr7g0233561 35.873 315 165 10 18 313 16 312 3.61e-54 184
Msa0136910 MtrunA17_Chr3g0131891 36.538 312 163 9 15 313 14 303 3.67e-54 184
Msa0136910 MtrunA17_Chr7g0233541 36.149 296 154 11 36 313 2 280 1.04e-49 171
Msa0136910 MtrunA17_Chr7g0233531 34.000 300 138 9 36 313 2 263 1.23e-43 155
Msa0136910 MtrunA17_Chr1g0163181 27.802 464 265 15 29 471 14 428 5.41e-43 157
Msa0136910 MtrunA17_Chr3g0103181 32.258 310 155 11 18 312 10 279 5.04e-42 150
Msa0136910 MtrunA17_Chr3g0103181 29.358 218 124 7 279 472 68 279 9.72e-13 68.6
Msa0136910 MtrunA17_Chr7g0233521 32.203 295 150 8 36 313 2 263 6.17e-42 150
Msa0136910 MtrunA17_Chr3g0103191 31.769 277 139 8 18 279 10 251 1.49e-38 140
Msa0136910 MtrunA17_Chr3g0103171 31.058 293 155 8 35 312 5 265 1.59e-38 140
Msa0136910 MtrunA17_Chr3g0103171 29.259 270 150 10 237 472 3 265 4.60e-17 81.3
Msa0136910 MtrunA17_Chr7g0233571 34.529 223 123 5 20 235 10 216 3.13e-35 130
Msa0136910 MtrunA17_Chr1g0163201 26.872 454 235 14 35 471 18 391 3.53e-35 135
Msa0136910 MtrunA17_Chr3g0103151 40.667 150 80 2 18 160 10 157 3.23e-33 123
Msa0136910 MtrunA17_Chr4g0067431 27.187 320 203 12 15 317 10 316 1.15e-31 124
Msa0136910 MtrunA17_Chr1g0163221 26.462 359 218 11 10 343 1 338 4.60e-31 123
Msa0136910 MtrunA17_Chr3g0103201 31.890 254 123 7 75 312 1 220 1.34e-28 112
Msa0136910 MtrunA17_Chr1g0163261 26.168 321 194 8 19 327 8 297 7.41e-27 110
Msa0136910 MtrunA17_Chr1g0163251 29.073 313 184 12 29 331 14 298 7.98e-26 108
Msa0136910 MtrunA17_Chr5g0429931 26.460 291 146 9 35 314 10 243 4.05e-25 103
Msa0136910 MtrunA17_Chr5g0419201 32.558 172 92 5 5 160 2 165 1.49e-24 100
Msa0136910 MtrunA17_Chr1g0163231 26.316 304 177 10 20 311 9 277 5.77e-23 99.0
Msa0136910 MtrunA17_Chr1g0154161 26.230 305 184 12 25 317 36 311 5.00e-21 94.0
Msa0136910 MtrunA17_Chr1g0163131 27.302 315 185 11 15 309 13 303 6.93e-21 93.2
Msa0136910 MtrunA17_Chr1g0154071 25.723 346 205 12 21 322 157 494 8.13e-21 95.1
Msa0136910 MtrunA17_Chr1g0171611 25.974 308 161 9 20 311 9 265 9.39e-21 92.4
Msa0136910 MtrunA17_Chr1g0163111 28.014 282 134 11 212 476 2 231 3.16e-20 89.4
Msa0136910 MtrunA17_Chr3g0077411 24.204 314 168 8 17 314 259 518 3.16e-20 93.6
Msa0136910 MtrunA17_Chr3g0077411 21.832 513 320 17 20 472 25 516 1.37e-19 91.7
Msa0136910 MtrunA17_Chr4g0033961 37.963 108 67 0 20 127 6 113 1.74e-19 90.5
Msa0136910 MtrunA17_Chr4g0033961 38.931 131 72 4 201 324 270 399 2.21e-18 87.4
Msa0136910 MtrunA17_Chr1g0154191 25.000 328 193 12 36 317 38 358 9.01e-19 87.8
Msa0136910 MtrunA17_Chr1g0154111 26.444 329 193 12 18 301 24 348 9.32e-19 89.4
Msa0136910 MtrunA17_Chr6g0467351 29.801 151 101 2 16 165 11 157 2.35e-18 84.0
Msa0136910 MtrunA17_Chr1g0154131 25.887 282 176 8 34 301 196 458 8.48e-18 85.9
Msa0136910 MtrunA17_Chr1g0162441 35.036 137 74 3 20 141 7 143 3.11e-17 84.0
Msa0136910 MtrunA17_Chr1g0162441 36.434 129 72 5 201 321 274 400 1.81e-15 78.6
Msa0136910 MtrunA17_Chr1g0163151 24.908 273 138 9 212 471 2 220 3.79e-17 80.5
Msa0136910 MtrunA17_Chr1g0154151 22.397 317 192 10 36 317 20 317 5.25e-16 79.0
Msa0136910 MtrunA17_Chr6g0476181 31.818 154 97 4 36 189 2 147 1.29e-15 74.3
Msa0136910 MtrunA17_Chr3g0077451 26.829 164 104 3 34 181 10 173 1.79e-15 75.9
Msa0136910 MtrunA17_Chr1g0163191 37.778 90 54 1 29 118 14 101 2.15e-15 73.9
Msa0136910 MtrunA17_Chr3g0077461 25.685 292 141 9 20 304 237 459 3.00e-15 77.8
Msa0136910 MtrunA17_Chr3g0077461 33.333 84 56 0 35 118 22 105 1.78e-12 69.3
Msa0136910 MtrunA17_Chr3g0103161 37.288 118 69 3 360 476 14 127 3.71e-15 72.0
Msa0136910 MtrunA17_Chr3g0103161 31.818 110 61 5 215 315 22 126 9.45e-11 59.3
Msa0136910 MtrunA17_Chr1g0163101 33.613 119 76 2 14 132 11 126 2.86e-14 71.2
Msa0136910 MtrunA17_Chr1g0163241 38.095 84 50 1 35 118 22 103 4.99e-14 70.1
Msa0136910 MtrunA17_Chr1g0163121 40.426 94 53 2 34 127 9 99 5.21e-14 69.3
Msa0136910 MtrunA17_Chr1g0163061 33.010 103 68 1 17 118 12 114 2.98e-13 70.1
Msa0136910 MtrunA17_Chr4g0009491 29.677 155 98 4 12 161 213 361 3.35e-12 68.2
Msa0136910 MtrunA17_Chr1g0162411 35.354 99 62 1 20 118 7 103 4.61e-12 67.8
Msa0136910 MtrunA17_Chr3g0077381 29.221 154 100 3 12 161 235 383 2.43e-11 65.5
Msa0136910 MtrunA17_Chr3g0077381 31.765 85 58 0 34 118 34 118 2.49e-11 65.5
Msa0136910 MtrunA17_Chr1g0163091 34.615 130 71 6 206 327 8 131 2.66e-11 62.0
Msa0136910 MtrunA17_Chr3g0077361 31.000 100 68 1 20 118 40 139 3.31e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0136910 AT3G18990.1 32.344 337 185 11 20 316 6 339 4.44e-43 155
Msa0136910 AT3G18990.2 32.047 337 186 11 20 316 6 339 4.27e-42 154
Msa0136910 AT1G49480.3 31.977 172 88 7 171 316 56 224 2.60e-17 81.3
Msa0136910 AT1G49480.3 35.897 117 66 4 362 473 109 221 5.19e-11 63.2
Msa0136910 AT1G49480.1 31.977 172 88 7 171 316 56 224 2.60e-17 81.3
Msa0136910 AT1G49480.1 35.897 117 66 4 362 473 109 221 5.19e-11 63.2
Msa0136910 AT1G49480.2 31.977 172 88 7 171 316 56 224 2.60e-17 81.3
Msa0136910 AT1G49480.2 35.897 117 66 4 362 473 109 221 5.19e-11 63.2
Msa0136910 AT4G01580.1 36.538 104 66 0 19 122 29 132 8.84e-17 79.0
Msa0136910 AT4G33280.1 26.667 300 197 10 34 320 37 326 2.42e-16 80.5
Msa0136910 AT3G18960.2 34.615 104 68 0 19 122 29 132 5.93e-16 76.6
Msa0136910 AT3G18960.1 34.615 104 68 0 19 122 29 132 8.98e-16 76.6
Msa0136910 AT1G49475.1 31.667 120 77 2 19 138 33 147 1.90e-14 72.4
Msa0136910 AT3G18960.3 35.227 88 57 0 35 122 8 95 1.73e-11 63.2
Msa0136910 AT4G31690.1 24.490 245 161 7 220 462 10 232 2.26e-11 64.7

Find 77 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACAATCCAATTGAAATTT+TGG 0.104888 1_3:+69136579 Msa0136910:CDS
CCTAAACTTGTCCAAGTTAA+AGG 0.118978 1_3:+69136724 Msa0136910:CDS
ATCTGATAATAATTTGAAAT+TGG 0.143719 1_3:+69137275 Msa0136910:CDS
CTCAAATTGATAGTGAAATT+TGG 0.167485 1_3:-69137949 None:intergenic
GAACTTGAAATTTGTGATTT+TGG 0.194976 1_3:-69136631 None:intergenic
GTGAGACTGTACATATTCTT+AGG 0.199034 1_3:-69135868 None:intergenic
GGAAATACATTTGACTAAAA+AGG 0.231817 1_3:+69136381 Msa0136910:CDS
CTTGACCATGCTTCACATTC+AGG 0.239372 1_3:-69136877 None:intergenic
TATCATGACAGCAGTGAAAA+AGG 0.253877 1_3:-69136969 None:intergenic
CCTTCACCCAACTTTGTTGC+TGG 0.270614 1_3:-69138270 None:intergenic
CCTTTAACTTGGACAAGTTT+AGG 0.279816 1_3:-69136724 None:intergenic
TGCTATTGATCATCTCAAAA+AGG 0.283820 1_3:-69137308 None:intergenic
ACCCAACTTTGTTGCTGGTT+CGG 0.316172 1_3:-69138265 None:intergenic
CGAAATCGCCCTTCAGGTCT+TGG 0.340066 1_3:+69137185 Msa0136910:CDS
TCAATTTGAGATCAAGTCAT+TGG 0.345291 1_3:+69137962 Msa0136910:CDS
TGCTCATCCAAAATTTCAAT+TGG 0.347442 1_3:-69136586 None:intergenic
TATTTCTCAGTGAAAGCATT+TGG 0.349857 1_3:-69136304 None:intergenic
TCTGATAATAATTTGAAATT+GGG 0.355411 1_3:+69137276 Msa0136910:CDS
GTACATATTCTTAGGGTTGC+TGG 0.358212 1_3:-69135860 None:intergenic
GACTGCAATGTTACAGTTTG+AGG 0.362858 1_3:+69138206 Msa0136910:CDS
TTCATTCTCGAGCAAGTACT+TGG 0.373540 1_3:+69138170 Msa0136910:CDS
GCAAAGCGAAATCGCCCTTC+AGG 0.399129 1_3:+69137179 Msa0136910:CDS
GAAATTGAATATCGTGTTGA+CGG 0.403003 1_3:+69136538 Msa0136910:CDS
TGTAGAAGAAAATAAATTGC+AGG 0.404615 1_3:+69138305 Msa0136910:CDS
AAGCACAACATCTTCCTTGT+TGG 0.409928 1_3:-69138355 None:intergenic
CAAAGTGGATGTGAATTGAT+TGG 0.417163 1_3:-69137920 None:intergenic
CTTCACATTCAGGCATTTAG+TGG 0.427188 1_3:-69136867 None:intergenic
ATATTTGGATTCAAAGGGGT+TGG 0.428451 1_3:+69136410 Msa0136910:CDS
ATTTCCCATTCTTTCTCATC+GGG 0.433534 1_3:-69136364 None:intergenic
ATGGTGATATTTGGATTCAA+AGG 0.444319 1_3:+69136404 Msa0136910:CDS
AATTTATTATACCTTTAACT+TGG 0.446187 1_3:-69136735 None:intergenic
AACTTTGTTGCTGGTTCGGA+TGG 0.454779 1_3:-69138261 None:intergenic
TCCGAACCAGCAACAAAGTT+GGG 0.458098 1_3:+69138264 Msa0136910:CDS
TGGTGATATTTGGATTCAAA+GGG 0.458234 1_3:+69136405 Msa0136910:CDS
GGTCTACTCTTCTTGTGAGA+AGG 0.460496 1_3:-69136610 None:intergenic
ATTCTTAGGGTTGCTGGTCA+TGG 0.460769 1_3:-69135854 None:intergenic
TTGGATTCAAAGGGGTTGGA+AGG 0.464926 1_3:+69136414 Msa0136910:CDS
AGACTCCCGATGAGAAAGAA+TGG 0.468520 1_3:+69136359 Msa0136910:CDS
CAATCAATTCACATCCACTT+TGG 0.477065 1_3:+69137921 Msa0136910:CDS
TCTTGGATGATGAGAGAACT+TGG 0.477954 1_3:+69137202 Msa0136910:CDS
TTTGAATCAGTTCATGGAGA+TGG 0.482390 1_3:+69137474 Msa0136910:intron
ACACATTATTCATTAGATCA+TGG 0.484576 1_3:+69136445 Msa0136910:CDS
TTCATGGAGATGGAATTAAC+TGG 0.486836 1_3:+69137484 Msa0136910:CDS
TATTTCCCATTCTTTCTCAT+CGG 0.492052 1_3:-69136365 None:intergenic
CAATTTGAGATCAAGTCATT+GGG 0.498692 1_3:+69137963 Msa0136910:CDS
TGAGACTGTACATATTCTTA+GGG 0.500244 1_3:-69135867 None:intergenic
GACTCCCGATGAGAAAGAAT+GGG 0.507920 1_3:+69136360 Msa0136910:CDS
TTGGATTACCTTGTGGTGGA+AGG 0.508508 1_3:-69137383 None:intergenic
ATCCGAACCAGCAACAAAGT+TGG 0.508654 1_3:+69138263 Msa0136910:CDS
GGATAATACATCACTTGTAC+AGG 0.514406 1_3:-69138240 None:intergenic
ACCTCAACAAATATTCAAGA+TGG 0.526205 1_3:+69135922 Msa0136910:CDS
AATTAGTACTTTAAAAGCAT+AGG 0.530987 1_3:-69137334 None:intergenic
GCTTTCACTGAGAAATATAG+TGG 0.532968 1_3:+69136310 Msa0136910:CDS
TCTCATCATCCAAGACCTGA+AGG 0.534348 1_3:-69137194 None:intergenic
CAACAAAGTTGGGTGAAGGT+TGG 0.542982 1_3:+69138274 Msa0136910:CDS
TGGATTACCTTGTGGTGGAA+GGG 0.545050 1_3:-69137382 None:intergenic
GAAGAAAATAAATTGCAGGC+TGG 0.548366 1_3:+69138309 Msa0136910:CDS
TCGGGAGTCTTGAGAAACAT+TGG 0.550403 1_3:-69136346 None:intergenic
AACTCAAAGAAATTCACTGC+AGG 0.555404 1_3:+69137855 Msa0136910:CDS
ATTAACTGGTTAGAAACTGC+AGG 0.556059 1_3:+69137498 Msa0136910:CDS
AAAGAAATTCACTGCAGGCT+AGG 0.559813 1_3:+69137860 Msa0136910:CDS
TCCATCTTGAATATTTGTTG+AGG 0.562475 1_3:-69135923 None:intergenic
TTCATTAGATCATGGTCACA+TGG 0.571925 1_3:+69136453 Msa0136910:CDS
CATCTTGGATTACCTTGTGG+TGG 0.585751 1_3:-69137387 None:intergenic
TCATTGGGATGTTTACAGAC+CGG 0.600053 1_3:+69137978 Msa0136910:CDS
GGTGATATTTGGATTCAAAG+GGG 0.604343 1_3:+69136406 Msa0136910:CDS
TTCATATGTTAATCAATACA+TGG 0.607228 1_3:+69136994 Msa0136910:CDS
CTCATCATCCAAGACCTGAA+GGG 0.610171 1_3:-69137193 None:intergenic
ATTGACACAAGTGAAGATGT+CGG 0.614793 1_3:+69136676 Msa0136910:CDS
TATGTAAACCTCAAAATGAG+AGG 0.618304 1_3:-69136497 None:intergenic
CCAGCAACAAAGTTGGGTGA+AGG 0.622086 1_3:+69138270 Msa0136910:CDS
ACGGTAATAATCAACATGAA+CGG 0.624679 1_3:+69136557 Msa0136910:CDS
TCATTCTCGAGCAAGTACTT+GGG 0.630141 1_3:+69138171 Msa0136910:CDS
CAACATTCCCTTCCACCACA+AGG 0.632695 1_3:+69137375 Msa0136910:CDS
TGAAGCATGGTCAAGAGCAG+AGG 0.641697 1_3:+69136885 Msa0136910:CDS
TTGGCATACCTCAGGAACGA+AGG 0.641759 1_3:+69137831 Msa0136910:intron
AAATGCCTGAATGTGAAGCA+TGG 0.702749 1_3:+69136872 Msa0136910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTATTATACCTTTAACT+TGG - chr1_3:69136738-69136757 None:intergenic 15.0%
!!! ACTTTTGTTCAAAATTTAAA+GGG - chr1_3:69137146-69137165 None:intergenic 15.0%
!!! TACTTTTGTTCAAAATTTAA+AGG - chr1_3:69137147-69137166 None:intergenic 15.0%
!!! ATCTGATAATAATTTGAAAT+TGG + chr1_3:69137275-69137294 Msa0136910:CDS 15.0%
!!! TCTGATAATAATTTGAAATT+GGG + chr1_3:69137276-69137295 Msa0136910:CDS 15.0%
!! AAAGATTACTAATAACTTTA+AGG - chr1_3:69137420-69137439 None:intergenic 15.0%
!!! TAAAGTTATTAGTAATCTTT+TGG + chr1_3:69137420-69137439 Msa0136910:intron 15.0%
!!! TAATACAAAATGTTGTTTAA+TGG + chr1_3:69137672-69137691 Msa0136910:intron 15.0%
!! ATCATACAGTACTTAAATTT+CGG - chr1_3:69136014-69136033 None:intergenic 20.0%
!!! TTTTCTTAGAAAGATACATA+TGG - chr1_3:69136133-69136152 None:intergenic 20.0%
!!! TCAATTCTCAATTTTTTAAG+TGG - chr1_3:69136661-69136680 None:intergenic 20.0%
!! AGGTATAATAAATTCATGAT+TGG + chr1_3:69136744-69136763 Msa0136910:intron 20.0%
!! TTCATATGTTAATCAATACA+TGG + chr1_3:69136994-69137013 Msa0136910:CDS 20.0%
!!! TTTTGAACAAAAGTATTTGA+AGG + chr1_3:69137152-69137171 Msa0136910:CDS 20.0%
!!! AATTAGTACTTTAAAAGCAT+AGG - chr1_3:69137337-69137356 None:intergenic 20.0%
!! AAAACTATTATGTCGTATAA+AGG + chr1_3:69137540-69137559 Msa0136910:intron 20.0%
!!! AATTGATTATATGCTTTTGT+TGG + chr1_3:69137812-69137831 Msa0136910:intron 20.0%
!! AATCACTAGATATATGTATT+TGG - chr1_3:69138032-69138051 None:intergenic 20.0%
! AAGAAAGAAAGAAAGAAAGA+AGG - chr1_3:69135968-69135987 None:intergenic 25.0%
!! TTGTTATGTTATGTTTTACG+CGG + chr1_3:69136040-69136059 Msa0136910:intron 25.0%
! ATTAAAAGTTCAAGTGAGTT+AGG - chr1_3:69136091-69136110 None:intergenic 25.0%
! GGAAATACATTTGACTAAAA+AGG + chr1_3:69136381-69136400 Msa0136910:CDS 25.0%
! ATACATTTGACTAAAAAGGA+TGG + chr1_3:69136385-69136404 Msa0136910:CDS 25.0%
! ACACATTATTCATTAGATCA+TGG + chr1_3:69136445-69136464 Msa0136910:CDS 25.0%
!! GAACAATCCAATTGAAATTT+TGG + chr1_3:69136579-69136598 Msa0136910:CDS 25.0%
!! GAACTTGAAATTTGTGATTT+TGG - chr1_3:69136634-69136653 None:intergenic 25.0%
! TGTATGAGAAAAATTAGTAG+CGG + chr1_3:69137237-69137256 Msa0136910:CDS 25.0%
!!! CTCAAATTGATAGTGAAATT+TGG - chr1_3:69137952-69137971 None:intergenic 25.0%
! CATAAATACATATTCCTTAC+CGG - chr1_3:69138000-69138019 None:intergenic 25.0%
!! GTTTCTTTTACAGTTAGTTA+GGG - chr1_3:69138125-69138144 None:intergenic 25.0%
!! TGTTTCTTTTACAGTTAGTT+AGG - chr1_3:69138126-69138145 None:intergenic 25.0%
! TGTAGAAGAAAATAAATTGC+AGG + chr1_3:69138305-69138324 Msa0136910:CDS 25.0%
TGAGACTGTACATATTCTTA+GGG - chr1_3:69135870-69135889 None:intergenic 30.0%
ACCTCAACAAATATTCAAGA+TGG + chr1_3:69135922-69135941 Msa0136910:CDS 30.0%
! TCCATCTTGAATATTTGTTG+AGG - chr1_3:69135926-69135945 None:intergenic 30.0%
!! TATTTCTCAGTGAAAGCATT+TGG - chr1_3:69136307-69136326 None:intergenic 30.0%
TATTTCCCATTCTTTCTCAT+CGG - chr1_3:69136368-69136387 None:intergenic 30.0%
CTAAAAAGGATGGTGATATT+TGG + chr1_3:69136395-69136414 Msa0136910:CDS 30.0%
ATGGTGATATTTGGATTCAA+AGG + chr1_3:69136404-69136423 Msa0136910:CDS 30.0%
TGGTGATATTTGGATTCAAA+GGG + chr1_3:69136405-69136424 Msa0136910:CDS 30.0%
TATGTAAACCTCAAAATGAG+AGG - chr1_3:69136500-69136519 None:intergenic 30.0%
GAAATTGAATATCGTGTTGA+CGG + chr1_3:69136538-69136557 Msa0136910:CDS 30.0%
ACGGTAATAATCAACATGAA+CGG + chr1_3:69136557-69136576 Msa0136910:CDS 30.0%
TGCTCATCCAAAATTTCAAT+TGG - chr1_3:69136589-69136608 None:intergenic 30.0%
! TTTAGGAATGTTTTGCGATT+TGG - chr1_3:69136710-69136729 None:intergenic 30.0%
! CTCTTCTGAGAAATTGAATA+TGG - chr1_3:69136837-69136856 None:intergenic 30.0%
CAAAATTTAAAGGGACATAC+TGG - chr1_3:69137137-69137156 None:intergenic 30.0%
TGCTATTGATCATCTCAAAA+AGG - chr1_3:69137311-69137330 None:intergenic 30.0%
! CTTTAAGGAAAAAACCATCT+TGG - chr1_3:69137405-69137424 None:intergenic 30.0%
TTCAACTTCAAATTCCCAAA+GGG - chr1_3:69137592-69137611 None:intergenic 30.0%
ATTCAACTTCAAATTCCCAA+AGG - chr1_3:69137593-69137612 None:intergenic 30.0%
! CTTAGCTTCAGAATTTTTGA+AGG - chr1_3:69137893-69137912 None:intergenic 30.0%
!!! GAAATTTGGTTTTTCCAAAG+TGG - chr1_3:69137938-69137957 None:intergenic 30.0%
! TCAATTTGAGATCAAGTCAT+TGG + chr1_3:69137962-69137981 Msa0136910:CDS 30.0%
! CAATTTGAGATCAAGTCATT+GGG + chr1_3:69137963-69137982 Msa0136910:CDS 30.0%
CTAGTGATTATGCTTAAAAC+TGG + chr1_3:69138043-69138062 Msa0136910:intron 30.0%
! AACATTGCAGTCTTCTTTTT+GGG - chr1_3:69138198-69138217 None:intergenic 30.0%
! TAACATTGCAGTCTTCTTTT+TGG - chr1_3:69138199-69138218 None:intergenic 30.0%
TACAGTTTGAGGAAAAATTG+TGG + chr1_3:69138217-69138236 Msa0136910:CDS 30.0%
! CTTGATGTTCATATTTTCAG+AGG + chr1_3:69138375-69138394 Msa0136910:CDS 30.0%
GTGAGACTGTACATATTCTT+AGG - chr1_3:69135871-69135890 None:intergenic 35.0%
GAAGGAAAGAATGCTTACAA+GGG - chr1_3:69135950-69135969 None:intergenic 35.0%
AGAAGGAAAGAATGCTTACA+AGG - chr1_3:69135951-69135970 None:intergenic 35.0%
GCTTTCACTGAGAAATATAG+TGG + chr1_3:69136310-69136329 Msa0136910:CDS 35.0%
ATTTCCCATTCTTTCTCATC+GGG - chr1_3:69136367-69136386 None:intergenic 35.0%
GGTGATATTTGGATTCAAAG+GGG + chr1_3:69136406-69136425 Msa0136910:CDS 35.0%
ATATTTGGATTCAAAGGGGT+TGG + chr1_3:69136410-69136429 Msa0136910:CDS 35.0%
TTCATTAGATCATGGTCACA+TGG + chr1_3:69136453-69136472 Msa0136910:CDS 35.0%
! TCAGAAAACCTCTCATTTTG+AGG + chr1_3:69136489-69136508 Msa0136910:CDS 35.0%
ATTGACACAAGTGAAGATGT+CGG + chr1_3:69136676-69136695 Msa0136910:CDS 35.0%
!! CCTTTAACTTGGACAAGTTT+AGG - chr1_3:69136727-69136746 None:intergenic 35.0%
CCTAAACTTGTCCAAGTTAA+AGG + chr1_3:69136724-69136743 Msa0136910:CDS 35.0%
ATCATGACAGCAGTGAAAAA+GGG - chr1_3:69136971-69136990 None:intergenic 35.0%
TATCATGACAGCAGTGAAAA+AGG - chr1_3:69136972-69136991 None:intergenic 35.0%
! TGAGAAAAATTAGTAGCGGA+TGG + chr1_3:69137241-69137260 Msa0136910:CDS 35.0%
TCATCCTTTGAATCAGTTCA+TGG + chr1_3:69137468-69137487 Msa0136910:intron 35.0%
! ATCTCCATGAACTGATTCAA+AGG - chr1_3:69137475-69137494 None:intergenic 35.0%
TTTGAATCAGTTCATGGAGA+TGG + chr1_3:69137474-69137493 Msa0136910:intron 35.0%
TTCATGGAGATGGAATTAAC+TGG + chr1_3:69137484-69137503 Msa0136910:CDS 35.0%
ATTAACTGGTTAGAAACTGC+AGG + chr1_3:69137498-69137517 Msa0136910:CDS 35.0%
ATCAAGCAGTATTGTCAAGA+TGG + chr1_3:69137611-69137630 Msa0136910:intron 35.0%
CGTGAGTCGTAAAAAAATAG+AGG - chr1_3:69137721-69137740 None:intergenic 35.0%
AACTCAAAGAAATTCACTGC+AGG + chr1_3:69137855-69137874 Msa0136910:CDS 35.0%
!! TCAGAATTTTTGAAGGAGCA+TGG - chr1_3:69137886-69137905 None:intergenic 35.0%
CAAAGTGGATGTGAATTGAT+TGG - chr1_3:69137923-69137942 None:intergenic 35.0%
CAATCAATTCACATCCACTT+TGG + chr1_3:69137921-69137940 Msa0136910:CDS 35.0%
GATTATGCTTAAAACTGGAG+TGG + chr1_3:69138048-69138067 Msa0136910:intron 35.0%
GGATAATACATCACTTGTAC+AGG - chr1_3:69138243-69138262 None:intergenic 35.0%
GAAGAAAATAAATTGCAGGC+TGG + chr1_3:69138309-69138328 Msa0136910:CDS 35.0%
GTACATATTCTTAGGGTTGC+TGG - chr1_3:69135863-69135882 None:intergenic 40.0%
CTTCACATTCAGGCATTTAG+TGG - chr1_3:69136870-69136889 None:intergenic 40.0%
AAATGCCTGAATGTGAAGCA+TGG + chr1_3:69136872-69136891 Msa0136910:CDS 40.0%
TCTTGGATGATGAGAGAACT+TGG + chr1_3:69137202-69137221 Msa0136910:CDS 40.0%
AACCATCTTGGATTACCTTG+TGG - chr1_3:69137393-69137412 None:intergenic 40.0%
AAAGAAATTCACTGCAGGCT+AGG + chr1_3:69137860-69137879 Msa0136910:CDS 40.0%
TCATTGGGATGTTTACAGAC+CGG + chr1_3:69137978-69137997 Msa0136910:CDS 40.0%
TTCATTCTCGAGCAAGTACT+TGG + chr1_3:69138170-69138189 Msa0136910:CDS 40.0%
TCATTCTCGAGCAAGTACTT+GGG + chr1_3:69138171-69138190 Msa0136910:CDS 40.0%
GACTGCAATGTTACAGTTTG+AGG + chr1_3:69138206-69138225 Msa0136910:CDS 40.0%
!! TGTTTTTGTGCTTGCCAACA+AGG + chr1_3:69138341-69138360 Msa0136910:CDS 40.0%
AAGCACAACATCTTCCTTGT+TGG - chr1_3:69138358-69138377 None:intergenic 40.0%
ATTCTTAGGGTTGCTGGTCA+TGG - chr1_3:69135857-69135876 None:intergenic 45.0%
GATTCCTTCCTTGTATCCTC+AGG + chr1_3:69136064-69136083 Msa0136910:intron 45.0%
AGTTAGGTCCTGAGGATACA+AGG - chr1_3:69136075-69136094 None:intergenic 45.0%
TTCAAGTGAGTTAGGTCCTG+AGG - chr1_3:69136083-69136102 None:intergenic 45.0%
TCGGGAGTCTTGAGAAACAT+TGG - chr1_3:69136349-69136368 None:intergenic 45.0%
AGACTCCCGATGAGAAAGAA+TGG + chr1_3:69136359-69136378 Msa0136910:CDS 45.0%
GACTCCCGATGAGAAAGAAT+GGG + chr1_3:69136360-69136379 Msa0136910:CDS 45.0%
TTGGATTCAAAGGGGTTGGA+AGG + chr1_3:69136414-69136433 Msa0136910:CDS 45.0%
GGTCTACTCTTCTTGTGAGA+AGG - chr1_3:69136613-69136632 None:intergenic 45.0%
CTTGACCATGCTTCACATTC+AGG - chr1_3:69136880-69136899 None:intergenic 45.0%
CTCATCATCCAAGACCTGAA+GGG - chr1_3:69137196-69137215 None:intergenic 45.0%
TCTCATCATCCAAGACCTGA+AGG - chr1_3:69137197-69137216 None:intergenic 45.0%
! TGGATTACCTTGTGGTGGAA+GGG - chr1_3:69137385-69137404 None:intergenic 45.0%
! TTGGATTACCTTGTGGTGGA+AGG - chr1_3:69137386-69137405 None:intergenic 45.0%
CATCTTGGATTACCTTGTGG+TGG - chr1_3:69137390-69137409 None:intergenic 45.0%
CACCACAAGGTAATCCAAGA+TGG + chr1_3:69137388-69137407 Msa0136910:intron 45.0%
ATCGTTGTTGTGACTCCCTT+TGG + chr1_3:69137574-69137593 Msa0136910:intron 45.0%
TCGTTGTTGTGACTCCCTTT+GGG + chr1_3:69137575-69137594 Msa0136910:intron 45.0%
! TGCTTTTGTTGGCATACCTC+AGG + chr1_3:69137823-69137842 Msa0136910:intron 45.0%
TTGAGTTTCCTTCGTTCCTG+AGG - chr1_3:69137842-69137861 None:intergenic 45.0%
!! AACTTTGTTGCTGGTTCGGA+TGG - chr1_3:69138264-69138283 None:intergenic 45.0%
ATCCGAACCAGCAACAAAGT+TGG + chr1_3:69138263-69138282 Msa0136910:CDS 45.0%
TCCGAACCAGCAACAAAGTT+GGG + chr1_3:69138264-69138283 Msa0136910:CDS 45.0%
! ACCCAACTTTGTTGCTGGTT+CGG - chr1_3:69138268-69138287 None:intergenic 45.0%
CAACAAAGTTGGGTGAAGGT+TGG + chr1_3:69138274-69138293 Msa0136910:CDS 45.0%
AGGTCCTGAGGATACAAGGA+AGG - chr1_3:69136071-69136090 None:intergenic 50.0%
TGAAGCATGGTCAAGAGCAG+AGG + chr1_3:69136885-69136904 Msa0136910:CDS 50.0%
CAACATTCCCTTCCACCACA+AGG + chr1_3:69137375-69137394 Msa0136910:CDS 50.0%
TTGGCATACCTCAGGAACGA+AGG + chr1_3:69137831-69137850 Msa0136910:intron 50.0%
GGGATGTTTACAGACCGGTA+AGG + chr1_3:69137983-69138002 Msa0136910:intron 50.0%
CCTTCACCCAACTTTGTTGC+TGG - chr1_3:69138273-69138292 None:intergenic 50.0%
CCAGCAACAAAGTTGGGTGA+AGG + chr1_3:69138270-69138289 Msa0136910:CDS 50.0%
GCAAAGCGAAATCGCCCTTC+AGG + chr1_3:69137179-69137198 Msa0136910:CDS 55.0%
CGAAATCGCCCTTCAGGTCT+TGG + chr1_3:69137185-69137204 Msa0136910:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 69135850 69138407 69135850 ID=Msa0136910;Name=Msa0136910
chr1_3 mRNA 69135850 69138407 69135850 ID=Msa0136910-mRNA-1;Parent=Msa0136910;Name=Msa0136910-mRNA-1;_AED=0.11;_eAED=0.12;_QI=0|0|0|1|1|1|7|0|476
chr1_3 exon 69135850 69135951 69135850 ID=Msa0136910-mRNA-1:exon:23853;Parent=Msa0136910-mRNA-1
chr1_3 exon 69136298 69136745 69136298 ID=Msa0136910-mRNA-1:exon:23854;Parent=Msa0136910-mRNA-1
chr1_3 exon 69136852 69137015 69136852 ID=Msa0136910-mRNA-1:exon:23855;Parent=Msa0136910-mRNA-1
chr1_3 exon 69137138 69137396 69137138 ID=Msa0136910-mRNA-1:exon:23856;Parent=Msa0136910-mRNA-1
chr1_3 exon 69137484 69137537 69137484 ID=Msa0136910-mRNA-1:exon:23857;Parent=Msa0136910-mRNA-1
chr1_3 exon 69137845 69137999 69137845 ID=Msa0136910-mRNA-1:exon:23858;Parent=Msa0136910-mRNA-1
chr1_3 exon 69138159 69138407 69138159 ID=Msa0136910-mRNA-1:exon:23859;Parent=Msa0136910-mRNA-1
chr1_3 CDS 69135850 69135951 69135850 ID=Msa0136910-mRNA-1:cds;Parent=Msa0136910-mRNA-1
chr1_3 CDS 69136298 69136745 69136298 ID=Msa0136910-mRNA-1:cds;Parent=Msa0136910-mRNA-1
chr1_3 CDS 69136852 69137015 69136852 ID=Msa0136910-mRNA-1:cds;Parent=Msa0136910-mRNA-1
chr1_3 CDS 69137138 69137396 69137138 ID=Msa0136910-mRNA-1:cds;Parent=Msa0136910-mRNA-1
chr1_3 CDS 69137484 69137537 69137484 ID=Msa0136910-mRNA-1:cds;Parent=Msa0136910-mRNA-1
chr1_3 CDS 69137845 69137999 69137845 ID=Msa0136910-mRNA-1:cds;Parent=Msa0136910-mRNA-1
chr1_3 CDS 69138159 69138407 69138159 ID=Msa0136910-mRNA-1:cds;Parent=Msa0136910-mRNA-1
Gene Sequence

>Msa0136910

ATGTCCATGACCAGCAACCCTAAGAATATGTACAGTCTCACTGCTTCTGCTATTCGTTTCTTTAAGATTGTTACCTCAACAAATATTCAAGATGGAACCCTTAGAATTCCAAATGCTTTCACTGAGAAATATAGTGGTGATTTATCAAAGCCAATGTTTCTCAAGACTCCCGATGAGAAAGAATGGGAAATACATTTGACTAAAAAGGATGGTGATATTTGGATTCAAAGGGGTTGGAAGGAATTTGCTACACATTATTCATTAGATCATGGTCACATGGTTGTGTTTCAATATCAGAAAACCTCTCATTTTGAGGTTTACATATTTGACAAGAGTACTTTTGAAATTGAATATCGTGTTGACGGTAATAATCAACATGAACGGAACAATCCAATTGAAATTTTGGATGAGCAACCTTCTCACAAGAAGAGTAGACCAAAATCACAAATTTCAAGTTCTCAACCACTTAAAAAATTGAGAATTGACACAAGTGAAGATGTCGGAACAAGCTCCAAATCGCAAAACATTCCTAAACTTGTCCAAGTTAAAGAAGAGATTGATTCTACCACTAAATGCCTGAATGTGAAGCATGGTCAAGAGCAGAGGAATTCAACTGCTAAAATTCTTGAAGCTTTAAACAAAGCAAAAAACTATGAATCTAACAATCCCTTTTTCACTGCTGTCATGATACATTCATATGTTAATCAATACATGTATGTCCCTTTAAATTTTGAACAAAAGTATTTGAAGGAACAGCAAAGCGAAATCGCCCTTCAGGTCTTGGATGATGAGAGAACTTGGATTGTTAAATATTGTATGAGAAAAATTAGTAGCGGATGGAAAACATTTGTATCTGATAATAATTTGAAATTGGGAGATGTTTGCCTTTTTGAGATGATCAATAGCAAATCCTATGCTTTTAAAGTACTAATTTTTCGAGTTGACGAAGAACAACATTCCCTTCCACCACAAGTTCATGGAGATGGAATTAACTGGTTAGAAACTGCAGGAATCACAGAAGTTGAAAGAACGAAGGAAACTCAAAGAAATTCACTGCAGGCTAGGCCATGCTCCTTCAAAAATTCTGAAGCTAAGAAAGAAGCCAATCAATTCACATCCACTTTGGAAAAACCAAATTTCACTATCAATTTGAGATCAAGTCATTGGGATGTTTACAGACCGCGTGTACGAAATTCATTCTCGAGCAAGTACTTGGGTCCCAAAAAGAAGACTGCAATGTTACAGTTTGAGGAAAAATTGTGGCCTGTACAAGTGATGTATTATCCATCCGAACCAGCAACAAAGTTGGGTGAAGGTTGGAGTCTGTTTGTAGAAGAAAATAAATTGCAGGCTGGAGATGTATGTGTTTTTGTGCTTGCCAACAAGGAAGATGTTGTGCTTGATGTTCATATTTTCAGAGGACGCAGTTAG
Protein sequence

>Msa0136910

MSMTSNPKNMYSLTASAIRFFKIVTSTNIQDGTLRIPNAFTEKYSGDLSKPMFLKTPDEKEWEIHLTKKDGDIWIQRGWKEFATHYSLDHGHMVVFQYQKTSHFEVYIFDKSTFEIEYRVDGNNQHERNNPIEILDEQPSHKKSRPKSQISSSQPLKKLRIDTSEDVGTSSKSQNIPKLVQVKEEIDSTTKCLNVKHGQEQRNSTAKILEALNKAKNYESNNPFFTAVMIHSYVNQYMYVPLNFEQKYLKEQQSEIALQVLDDERTWIVKYCMRKISSGWKTFVSDNNLKLGDVCLFEMINSKSYAFKVLIFRVDEEQHSLPPQVHGDGINWLETAGITEVERTKETQRNSLQARPCSFKNSEAKKEANQFTSTLEKPNFTINLRSSHWDVYRPRVRNSFSSKYLGPKKKTAMLQFEEKLWPVQVMYYPSEPATKLGEGWSLFVEENKLQAGDVCVFVLANKEDVVLDVHIFRGRS*