Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0141590 | A0A396JLZ7 | 93.985 | 133 | 7 | 1 | 1 | 133 | 1 | 132 | 5.14e-85 | 256 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0141590 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0141590 | MtrunA17_Chr1g0163091 | 93.985 | 133 | 7 | 1 | 1 | 133 | 1 | 132 | 9.90e-89 | 256 |
| Msa0141590 | MtrunA17_Chr1g0162151 | 76.692 | 133 | 30 | 1 | 1 | 133 | 1 | 132 | 6.31e-64 | 195 |
| Msa0141590 | MtrunA17_Chr1g0163251 | 54.286 | 140 | 63 | 1 | 2 | 141 | 163 | 301 | 1.38e-44 | 151 |
| Msa0141590 | MtrunA17_Chr1g0163201 | 42.012 | 169 | 86 | 2 | 1 | 168 | 165 | 322 | 1.88e-38 | 135 |
| Msa0141590 | MtrunA17_Chr1g0163181 | 39.037 | 187 | 90 | 4 | 1 | 169 | 180 | 360 | 8.37e-38 | 134 |
| Msa0141590 | MtrunA17_Chr1g0163261 | 51.852 | 135 | 60 | 2 | 1 | 131 | 161 | 294 | 1.44e-37 | 130 |
| Msa0141590 | MtrunA17_Chr1g0163151 | 38.824 | 170 | 87 | 3 | 1 | 170 | 1 | 153 | 1.28e-33 | 118 |
| Msa0141590 | MtrunA17_Chr6g0476171 | 45.732 | 164 | 70 | 6 | 3 | 159 | 12 | 163 | 2.99e-33 | 118 |
| Msa0141590 | MtrunA17_Chr1g0163111 | 41.216 | 148 | 71 | 3 | 1 | 143 | 1 | 137 | 5.34e-31 | 112 |
| Msa0141590 | MtrunA17_Chr1g0163231 | 50.794 | 126 | 54 | 4 | 1 | 122 | 160 | 281 | 6.07e-30 | 110 |
| Msa0141590 | MtrunA17_Chr1g0171611 | 49.558 | 113 | 53 | 3 | 14 | 122 | 157 | 269 | 7.58e-28 | 105 |
| Msa0141590 | MtrunA17_Chr5g0419211 | 40.217 | 92 | 54 | 1 | 42 | 132 | 11 | 102 | 1.68e-18 | 76.3 |
| Msa0141590 | MtrunA17_Chr1g0163071 | 29.730 | 111 | 70 | 2 | 23 | 132 | 196 | 299 | 4.70e-14 | 68.2 |
| Msa0141590 | MtrunA17_Chr1g0208771 | 32.886 | 149 | 88 | 5 | 8 | 152 | 182 | 322 | 6.71e-14 | 68.6 |
| Msa0141590 | MtrunA17_Chr8g0390841 | 34.921 | 126 | 71 | 5 | 11 | 132 | 226 | 344 | 7.33e-14 | 68.6 |
| Msa0141590 | MtrunA17_Chr3g0131891 | 35.922 | 103 | 61 | 3 | 23 | 120 | 201 | 303 | 2.60e-12 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 30 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACTTGGATTTCCATTAT+TGG | 0.230784 | 1_3:-76784702 | None:intergenic |
| AACTTGCAAGTTATTATCTT+TGG | 0.260722 | 1_3:-76784993 | None:intergenic |
| GAGATATCTTCAACAAATTC+AGG | 0.324285 | 1_3:+76785259 | Msa0141590:CDS |
| TCGAAGGGATTGCAAGGTTA+AGG | 0.333047 | 1_3:+76784882 | Msa0141590:CDS |
| TATTCTCTTGAAAACTTAGT+TGG | 0.372973 | 1_3:-76784848 | None:intergenic |
| CGAAGGGATTGCAAGGTTAA+GGG | 0.387959 | 1_3:+76784883 | Msa0141590:CDS |
| CCTTGCAATTTGTTAGGGCT+TGG | 0.389952 | 1_3:-76785094 | None:intergenic |
| GTTAATGAGAAATCCAATAA+TGG | 0.406882 | 1_3:+76784689 | Msa0141590:CDS |
| AATGTAAACTTACACATAAA+CGG | 0.413291 | 1_3:-76784757 | None:intergenic |
| TTCTATTGCTCGAGTTAGAA+AGG | 0.418117 | 1_3:+76785069 | Msa0141590:CDS |
| GTCAATTTAAGTTCAAAACT+TGG | 0.428551 | 1_3:-76784716 | None:intergenic |
| AGATGTTCTATTCTAAATGT+AGG | 0.453577 | 1_3:+76784956 | Msa0141590:CDS |
| GTTCTATTCTAAATGTAGGT+TGG | 0.464202 | 1_3:+76784960 | Msa0141590:CDS |
| GGGTTGGTGAGGATAGAGCT+TGG | 0.522308 | 1_3:+76784903 | Msa0141590:CDS |
| TGGTGAGGATAGAGCTTGGA+AGG | 0.526001 | 1_3:+76784907 | Msa0141590:CDS |
| TGCAAGGTTAAGGGTTGGTG+AGG | 0.534060 | 1_3:+76784892 | Msa0141590:CDS |
| AAAGATAATAACTTGCAAGT+TGG | 0.545014 | 1_3:+76784995 | Msa0141590:CDS |
| ATAGAAATAGTAAATGAAAG+TGG | 0.549884 | 1_3:-76785052 | None:intergenic |
| AACTTACACATAAACGGCCA+TGG | 0.550419 | 1_3:-76784751 | None:intergenic |
| AATACTTGAACGAGTTCGAA+GGG | 0.571263 | 1_3:+76784867 | Msa0141590:CDS |
| AATTAATCATACCGGGTAGG+AGG | 0.574343 | 1_3:-76785329 | None:intergenic |
| GAATACTTGAACGAGTTCGA+AGG | 0.582457 | 1_3:+76784866 | Msa0141590:CDS |
| CCAAGCCCTAACAAATTGCA+AGG | 0.590895 | 1_3:+76785094 | Msa0141590:CDS |
| GGGATTGCAAGGTTAAGGGT+TGG | 0.591648 | 1_3:+76784887 | Msa0141590:CDS |
| GTGTTGTTCAATAACTGAAG+TGG | 0.593272 | 1_3:+76784667 | Msa0141590:CDS |
| ACGAGTTCGAAGGGATTGCA+AGG | 0.614875 | 1_3:+76784876 | Msa0141590:CDS |
| TTGACACGAAGTTATGTCCA+TGG | 0.615137 | 1_3:+76784734 | Msa0141590:CDS |
| AGTAATTAATCATACCGGGT+AGG | 0.623102 | 1_3:-76785332 | None:intergenic |
| ATATCTTCAACAAATTCAGG+TGG | 0.668634 | 1_3:+76785262 | Msa0141590:CDS |
| GCATGACTAAGAAAGCAAAG+AGG | 0.728766 | 1_3:+76784645 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATTACTTACATAAAATG+TGG | + | chr1_3:76785346-76785365 | Msa0141590:intron | 15.0% |
| !! | TTAATTACTTACATAAAATG+TGG | + | chr1_3:76785346-76785365 | Msa0141590:intron | 15.0% |
| !! | AATGTAAACTTACACATAAA+CGG | - | chr1_3:76784760-76784779 | None:intergenic | 20.0% |
| !! | ATAGAAATAGTAAATGAAAG+TGG | - | chr1_3:76785055-76785074 | None:intergenic | 20.0% |
| !! | AAATGTTGATCAAATATCAA+TGG | - | chr1_3:76785178-76785197 | None:intergenic | 20.0% |
| !!! | AATTCCTTTTATAACTTTCT+AGG | + | chr1_3:76785226-76785245 | Msa0141590:intron | 20.0% |
| !! | ATAACCTAGAAAGTTATAAA+AGG | - | chr1_3:76785233-76785252 | None:intergenic | 20.0% |
| !! | ATGTAAGTAATTAATCATAC+CGG | - | chr1_3:76785340-76785359 | None:intergenic | 20.0% |
| !! | AATGTAAACTTACACATAAA+CGG | - | chr1_3:76784760-76784779 | None:intergenic | 20.0% |
| !! | ATAGAAATAGTAAATGAAAG+TGG | - | chr1_3:76785055-76785074 | None:intergenic | 20.0% |
| !! | AAATGTTGATCAAATATCAA+TGG | - | chr1_3:76785178-76785197 | None:intergenic | 20.0% |
| !!! | AATTCCTTTTATAACTTTCT+AGG | + | chr1_3:76785226-76785245 | Msa0141590:intron | 20.0% |
| !! | ATAACCTAGAAAGTTATAAA+AGG | - | chr1_3:76785233-76785252 | None:intergenic | 20.0% |
| !! | ATGTAAGTAATTAATCATAC+CGG | - | chr1_3:76785340-76785359 | None:intergenic | 20.0% |
| ! | GTTAATGAGAAATCCAATAA+TGG | + | chr1_3:76784689-76784708 | Msa0141590:CDS | 25.0% |
| ! | AAAACTTGGATTTCCATTAT+TGG | - | chr1_3:76784705-76784724 | None:intergenic | 25.0% |
| !! | GTCAATTTAAGTTCAAAACT+TGG | - | chr1_3:76784719-76784738 | None:intergenic | 25.0% |
| ! | TATTCTCTTGAAAACTTAGT+TGG | - | chr1_3:76784851-76784870 | None:intergenic | 25.0% |
| ! | AGATGTTCTATTCTAAATGT+AGG | + | chr1_3:76784956-76784975 | Msa0141590:CDS | 25.0% |
| ! | AACTTGCAAGTTATTATCTT+TGG | - | chr1_3:76784996-76785015 | None:intergenic | 25.0% |
| !! | AAAGATAATAACTTGCAAGT+TGG | + | chr1_3:76784995-76785014 | Msa0141590:CDS | 25.0% |
| !!! | AAAAACCTTGCAATTTGTTA+GGG | - | chr1_3:76785102-76785121 | None:intergenic | 25.0% |
| ! | TGTAAGTAATTAATCATACC+GGG | - | chr1_3:76785339-76785358 | None:intergenic | 25.0% |
| ! | GTTAATGAGAAATCCAATAA+TGG | + | chr1_3:76784689-76784708 | Msa0141590:CDS | 25.0% |
| ! | AAAACTTGGATTTCCATTAT+TGG | - | chr1_3:76784705-76784724 | None:intergenic | 25.0% |
| !! | GTCAATTTAAGTTCAAAACT+TGG | - | chr1_3:76784719-76784738 | None:intergenic | 25.0% |
| ! | TATTCTCTTGAAAACTTAGT+TGG | - | chr1_3:76784851-76784870 | None:intergenic | 25.0% |
| ! | AGATGTTCTATTCTAAATGT+AGG | + | chr1_3:76784956-76784975 | Msa0141590:CDS | 25.0% |
| ! | AACTTGCAAGTTATTATCTT+TGG | - | chr1_3:76784996-76785015 | None:intergenic | 25.0% |
| !! | AAAGATAATAACTTGCAAGT+TGG | + | chr1_3:76784995-76785014 | Msa0141590:CDS | 25.0% |
| !!! | AAAAACCTTGCAATTTGTTA+GGG | - | chr1_3:76785102-76785121 | None:intergenic | 25.0% |
| ! | TGTAAGTAATTAATCATACC+GGG | - | chr1_3:76785339-76785358 | None:intergenic | 25.0% |
| GTTCTATTCTAAATGTAGGT+TGG | + | chr1_3:76784960-76784979 | Msa0141590:CDS | 30.0% | |
| ! | GAAAAACCTTGCAATTTGTT+AGG | - | chr1_3:76785103-76785122 | None:intergenic | 30.0% |
| GAGATATCTTCAACAAATTC+AGG | + | chr1_3:76785259-76785278 | Msa0141590:CDS | 30.0% | |
| ATATCTTCAACAAATTCAGG+TGG | + | chr1_3:76785262-76785281 | Msa0141590:CDS | 30.0% | |
| GTTCTATTCTAAATGTAGGT+TGG | + | chr1_3:76784960-76784979 | Msa0141590:CDS | 30.0% | |
| ! | GAAAAACCTTGCAATTTGTT+AGG | - | chr1_3:76785103-76785122 | None:intergenic | 30.0% |
| GAGATATCTTCAACAAATTC+AGG | + | chr1_3:76785259-76785278 | Msa0141590:CDS | 30.0% | |
| ATATCTTCAACAAATTCAGG+TGG | + | chr1_3:76785262-76785281 | Msa0141590:CDS | 30.0% | |
| ! | GTGTTGTTCAATAACTGAAG+TGG | + | chr1_3:76784667-76784686 | Msa0141590:CDS | 35.0% |
| CAAACCAAGAATTGAAACAG+TGG | + | chr1_3:76784822-76784841 | Msa0141590:intron | 35.0% | |
| GTATCCACTGTTTCAATTCT+TGG | - | chr1_3:76784829-76784848 | None:intergenic | 35.0% | |
| AATACTTGAACGAGTTCGAA+GGG | + | chr1_3:76784867-76784886 | Msa0141590:CDS | 35.0% | |
| TTCTATTGCTCGAGTTAGAA+AGG | + | chr1_3:76785069-76785088 | Msa0141590:CDS | 35.0% | |
| AGTAATTAATCATACCGGGT+AGG | - | chr1_3:76785335-76785354 | None:intergenic | 35.0% | |
| ! | GTGTTGTTCAATAACTGAAG+TGG | + | chr1_3:76784667-76784686 | Msa0141590:CDS | 35.0% |
| CAAACCAAGAATTGAAACAG+TGG | + | chr1_3:76784822-76784841 | Msa0141590:intron | 35.0% | |
| GTATCCACTGTTTCAATTCT+TGG | - | chr1_3:76784829-76784848 | None:intergenic | 35.0% | |
| AATACTTGAACGAGTTCGAA+GGG | + | chr1_3:76784867-76784886 | Msa0141590:CDS | 35.0% | |
| TTCTATTGCTCGAGTTAGAA+AGG | + | chr1_3:76785069-76785088 | Msa0141590:CDS | 35.0% | |
| AGTAATTAATCATACCGGGT+AGG | - | chr1_3:76785335-76785354 | None:intergenic | 35.0% | |
| TTGACACGAAGTTATGTCCA+TGG | + | chr1_3:76784734-76784753 | Msa0141590:CDS | 40.0% | |
| AACTTACACATAAACGGCCA+TGG | - | chr1_3:76784754-76784773 | None:intergenic | 40.0% | |
| GAATACTTGAACGAGTTCGA+AGG | + | chr1_3:76784866-76784885 | Msa0141590:CDS | 40.0% | |
| ! | TTTTGTGAATTCCTCCTACC+CGG | + | chr1_3:76785318-76785337 | Msa0141590:CDS | 40.0% |
| AATTAATCATACCGGGTAGG+AGG | - | chr1_3:76785332-76785351 | None:intergenic | 40.0% | |
| CATCAGGTACAAACTGCAAA+TGG | - | chr1_3:76785421-76785440 | None:intergenic | 40.0% | |
| TTGACACGAAGTTATGTCCA+TGG | + | chr1_3:76784734-76784753 | Msa0141590:CDS | 40.0% | |
| AACTTACACATAAACGGCCA+TGG | - | chr1_3:76784754-76784773 | None:intergenic | 40.0% | |
| GAATACTTGAACGAGTTCGA+AGG | + | chr1_3:76784866-76784885 | Msa0141590:CDS | 40.0% | |
| ! | TTTTGTGAATTCCTCCTACC+CGG | + | chr1_3:76785318-76785337 | Msa0141590:CDS | 40.0% |
| AATTAATCATACCGGGTAGG+AGG | - | chr1_3:76785332-76785351 | None:intergenic | 40.0% | |
| CATCAGGTACAAACTGCAAA+TGG | - | chr1_3:76785421-76785440 | None:intergenic | 40.0% | |
| TCGAAGGGATTGCAAGGTTA+AGG | + | chr1_3:76784882-76784901 | Msa0141590:CDS | 45.0% | |
| CGAAGGGATTGCAAGGTTAA+GGG | + | chr1_3:76784883-76784902 | Msa0141590:CDS | 45.0% | |
| !! | CCTTGCAATTTGTTAGGGCT+TGG | - | chr1_3:76785097-76785116 | None:intergenic | 45.0% |
| CCAAGCCCTAACAAATTGCA+AGG | + | chr1_3:76785094-76785113 | Msa0141590:CDS | 45.0% | |
| TCGAAGGGATTGCAAGGTTA+AGG | + | chr1_3:76784882-76784901 | Msa0141590:CDS | 45.0% | |
| CGAAGGGATTGCAAGGTTAA+GGG | + | chr1_3:76784883-76784902 | Msa0141590:CDS | 45.0% | |
| !! | CCTTGCAATTTGTTAGGGCT+TGG | - | chr1_3:76785097-76785116 | None:intergenic | 45.0% |
| CCAAGCCCTAACAAATTGCA+AGG | + | chr1_3:76785094-76785113 | Msa0141590:CDS | 45.0% | |
| ACGAGTTCGAAGGGATTGCA+AGG | + | chr1_3:76784876-76784895 | Msa0141590:CDS | 50.0% | |
| GGGATTGCAAGGTTAAGGGT+TGG | + | chr1_3:76784887-76784906 | Msa0141590:CDS | 50.0% | |
| !! | TGCAAGGTTAAGGGTTGGTG+AGG | + | chr1_3:76784892-76784911 | Msa0141590:CDS | 50.0% |
| TGGTGAGGATAGAGCTTGGA+AGG | + | chr1_3:76784907-76784926 | Msa0141590:CDS | 50.0% | |
| ACGAGTTCGAAGGGATTGCA+AGG | + | chr1_3:76784876-76784895 | Msa0141590:CDS | 50.0% | |
| GGGATTGCAAGGTTAAGGGT+TGG | + | chr1_3:76784887-76784906 | Msa0141590:CDS | 50.0% | |
| !! | TGCAAGGTTAAGGGTTGGTG+AGG | + | chr1_3:76784892-76784911 | Msa0141590:CDS | 50.0% |
| TGGTGAGGATAGAGCTTGGA+AGG | + | chr1_3:76784907-76784926 | Msa0141590:CDS | 50.0% | |
| !! | GGGTTGGTGAGGATAGAGCT+TGG | + | chr1_3:76784903-76784922 | Msa0141590:CDS | 55.0% |
| !! | GGGTTGGTGAGGATAGAGCT+TGG | + | chr1_3:76784903-76784922 | Msa0141590:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_3 | gene | 76784647 | 76785454 | 76784647 | ID=Msa0141590;Name=Msa0141590 |
| chr1_3 | mRNA | 76784647 | 76785454 | 76784647 | ID=Msa0141590-mRNA-1;Parent=Msa0141590;Name=Msa0141590-mRNA-1;_AED=0.38;_eAED=0.39;_QI=0|0|0|0.5|1|1|4|0|170 |
| chr1_3 | exon | 76784647 | 76784766 | 76784647 | ID=Msa0141590-mRNA-1:exon:26762;Parent=Msa0141590-mRNA-1 |
| chr1_3 | exon | 76784842 | 76785115 | 76784842 | ID=Msa0141590-mRNA-1:exon:26763;Parent=Msa0141590-mRNA-1 |
| chr1_3 | exon | 76785248 | 76785339 | 76785248 | ID=Msa0141590-mRNA-1:exon:26764;Parent=Msa0141590-mRNA-1 |
| chr1_3 | exon | 76785428 | 76785454 | 76785428 | ID=Msa0141590-mRNA-1:exon:26765;Parent=Msa0141590-mRNA-1 |
| chr1_3 | CDS | 76784647 | 76784766 | 76784647 | ID=Msa0141590-mRNA-1:cds;Parent=Msa0141590-mRNA-1 |
| chr1_3 | CDS | 76784842 | 76785115 | 76784842 | ID=Msa0141590-mRNA-1:cds;Parent=Msa0141590-mRNA-1 |
| chr1_3 | CDS | 76785248 | 76785339 | 76785248 | ID=Msa0141590-mRNA-1:cds;Parent=Msa0141590-mRNA-1 |
| chr1_3 | CDS | 76785428 | 76785454 | 76785428 | ID=Msa0141590-mRNA-1:cds;Parent=Msa0141590-mRNA-1 |
| Gene Sequence |
| Protein sequence |
