AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0143260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0143260 MtrunA17_Chr7g0266301 95.425 153 7 0 1 153 1 153 7.16e-105 297
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0143260 AT5G15770.1 71.724 145 41 0 7 151 3 147 8.60e-73 216

Find 92 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGAACTATCTTATTATTT+TGG 0.145935 1_4:+2164891 Msa0143260:three_prime_UTR
ATAGGACAAGGTTGTCTTTA+TGG 0.149759 1_4:+2164288 Msa0143260:three_prime_UTR
CCCCGTTGCAATAATCTTTC+CGG 0.198555 1_4:-2163204 None:intergenic
TGGTGGGTTGGACATTATTC+TGG 0.237754 1_4:+2164827 Msa0143260:three_prime_UTR
ATTCTTTGTTACAGCTCTTA+TGG 0.246528 1_4:+2164625 Msa0143260:three_prime_UTR
GTCTGTAATCTCTAATCTTC+TGG 0.252175 1_4:-2163042 None:intergenic
AGTACTTCAAGATGTCTTTA+AGG 0.262133 1_4:+2163913 Msa0143260:three_prime_UTR
AAAGGCATTGAAAGAATAAA+AGG 0.271574 1_4:-2163748 None:intergenic
AATTTGCCTGTTCAATGATT+TGG 0.272978 1_4:+2164558 Msa0143260:three_prime_UTR
CTTGAAGTACTTACGGAAAT+AGG 0.282874 1_4:-2163901 None:intergenic
CAGCAGTACATGTTCCTTCC+TGG 0.283965 1_4:+2164709 Msa0143260:three_prime_UTR
CTCTTATGGTACATTTGAAA+TGG 0.294554 1_4:+2164639 Msa0143260:three_prime_UTR
TCGTTCTGTTGGGTGTTATA+AGG 0.300594 1_4:+2163358 Msa0143260:CDS
AATGGGACCTTGATGCTCTT+TGG 0.301050 1_4:+2164657 Msa0143260:three_prime_UTR
TCCATGATTATGTCATTATA+CGG 0.317864 1_4:-2164770 None:intergenic
TTCAATGATTTGGGTGCTTA+AGG 0.318125 1_4:+2164568 Msa0143260:three_prime_UTR
ATTTGCCTGTTCAATGATTT+GGG 0.332911 1_4:+2164559 Msa0143260:three_prime_UTR
TCTTGATTCTTCTATTAAAC+AGG 0.346474 1_4:+2164802 Msa0143260:three_prime_UTR
TAAGAAATTGTGGTAAAGTT+GGG 0.348212 1_4:+2163252 Msa0143260:CDS
GGTTTACTAGATTGAGTTGA+TGG 0.350822 1_4:+2164730 Msa0143260:three_prime_UTR
TTAAGAAATTGTGGTAAAGT+TGG 0.366943 1_4:+2163251 Msa0143260:CDS
ACCGATCATGCTCGTTCTGT+TGG 0.371791 1_4:+2163347 Msa0143260:CDS
AGTGCTCGTGGGAAACAATT+GGG 0.376890 1_4:+2163302 Msa0143260:CDS
TCCATTTATTACAAAAGTTA+AGG 0.378259 1_4:+2164525 Msa0143260:three_prime_UTR
GACTAAACAAGCACATGTTT+CGG 0.406211 1_4:-2164190 None:intergenic
GCCGGAAAGATTATTGCAAC+GGG 0.410196 1_4:+2163203 Msa0143260:CDS
TCTGTTATTTACATGTGTTA+TGG 0.410531 1_4:+2164072 Msa0143260:three_prime_UTR
ATGCTCTTTGGAAATGAAAT+CGG 0.416361 1_4:+2164669 Msa0143260:three_prime_UTR
AAGTGCTCGTGGGAAACAAT+TGG 0.427736 1_4:+2163301 Msa0143260:CDS
CTCACTGTTATCTGTACTCT+TGG 0.434639 1_4:+2164135 Msa0143260:three_prime_UTR
GCTATTGTAGCCTGACCTCT+TGG 0.434948 1_4:+2164157 Msa0143260:three_prime_UTR
TAATCTTTCACTAAAAGAAA+TGG 0.436591 1_4:-2163997 None:intergenic
AACATTATTCGTAGTACATA+AGG 0.443215 1_4:-2163969 None:intergenic
CCGATCATGCTCGTTCTGTT+GGG 0.443895 1_4:+2163348 Msa0143260:CDS
GTTGTGCATAGTGTTCATTT+TGG 0.445522 1_4:+2163877 Msa0143260:three_prime_UTR
CCATTGATGGTAGTTAATAA+AGG 0.447441 1_4:-2163766 None:intergenic
AGTCTCTGATAAGGAATTTG+AGG 0.451854 1_4:+2163115 Msa0143260:CDS
GATCAGAATTACAGCTGAAC+CGG 0.453557 1_4:-2164335 None:intergenic
TCTTATGGTACATTTGAAAT+GGG 0.455229 1_4:+2164640 Msa0143260:three_prime_UTR
AAGACATCTTGAAGTACTTA+CGG 0.459475 1_4:-2163908 None:intergenic
CCTTTATTAACTACCATCAA+TGG 0.464898 1_4:+2163766 Msa0143260:CDS
TTGTGATTCAGTCTCTGATA+AGG 0.468883 1_4:+2163106 Msa0143260:CDS
AAGCACCCAAATCATTGAAC+AGG 0.470933 1_4:-2164564 None:intergenic
TTTCGAGAGGTTGATTCACT+TGG 0.471380 1_4:+2163143 Msa0143260:CDS
ATGAACACTATGCACAACTC+TGG 0.493471 1_4:-2163872 None:intergenic
CGATTGCAGTGTTGAGAACA+AGG 0.494534 1_4:+2163388 Msa0143260:CDS
CTATGAGAAATGTGGTTTCA+AGG 0.496011 1_4:+2163415 Msa0143260:CDS
TTCTATTAAACAGGTGTGGT+GGG 0.498747 1_4:+2164811 Msa0143260:three_prime_UTR
ATTAAACAGGTGTGGTGGGT+TGG 0.504335 1_4:+2164815 Msa0143260:three_prime_UTR
CTTCTATTAAACAGGTGTGG+TGG 0.504663 1_4:+2164810 Msa0143260:three_prime_UTR
GTTATTGAAGATGAGATTGC+CGG 0.507961 1_4:+2163185 Msa0143260:CDS
TTTGATAAGTTGATTAACAT+AGG 0.509680 1_4:+2164270 Msa0143260:three_prime_UTR
GTACATAAGGCGATGTATGA+TGG 0.511132 1_4:-2163956 None:intergenic
GAATTATGAGGACAAGCATA+TGG 0.517162 1_4:+2164590 Msa0143260:three_prime_UTR
TTGCACATAGCCAAGAGGTC+AGG 0.517249 1_4:-2164167 None:intergenic
ACCGTATAATGACATAATCA+TGG 0.518108 1_4:+2164769 Msa0143260:three_prime_UTR
CCCAACAGAACGAGCATGAT+CGG 0.532044 1_4:-2163348 None:intergenic
GAACTGGTGGCGTCAATATG+TGG 0.532781 1_4:-2164237 None:intergenic
TCAACTGCGACAATATTTGT+AGG 0.535633 1_4:+2164411 Msa0143260:three_prime_UTR
GTCATAGAACTCTAGAAAGC+AGG 0.537605 1_4:-2163823 None:intergenic
TTTATTACAAAAGTTAAGGA+AGG 0.538052 1_4:+2164529 Msa0143260:three_prime_UTR
TGCCGGAAAGATTATTGCAA+CGG 0.539458 1_4:+2163202 Msa0143260:CDS
TTCATTTCCAAAGAGCATCA+AGG 0.542913 1_4:-2164664 None:intergenic
GTTTACTAGATTGAGTTGAT+GGG 0.550046 1_4:+2164731 Msa0143260:three_prime_UTR
AGCTGTAACAAAGAATTTCG+AGG 0.550946 1_4:-2164618 None:intergenic
ACAATGGTAGGAGCCATTGA+TGG 0.564879 1_4:-2163779 None:intergenic
CAAGGAGAAAGAAGTTCAGA+TGG 0.566779 1_4:+2163433 Msa0143260:CDS
ATTCTTCTATTAAACAGGTG+TGG 0.567164 1_4:+2164807 Msa0143260:three_prime_UTR
AATGAAATCGGTAGTGTGGT+AGG 0.569963 1_4:+2164681 Msa0143260:three_prime_UTR
AACTGGTGGCGTCAATATGT+GGG 0.574135 1_4:-2164236 None:intergenic
CAAACATCCACTACAATGGT+AGG 0.582996 1_4:-2163791 None:intergenic
TGGGTGCTTAAGGAATTATG+AGG 0.585630 1_4:+2164578 Msa0143260:three_prime_UTR
GACAATATTTGTAGGACGGA+GGG 0.592262 1_4:+2164419 Msa0143260:three_prime_UTR
AATGGCTCCTACCATTGTAG+TGG 0.602674 1_4:+2163784 Msa0143260:CDS
TCAACTCAATCTAGTAAACC+AGG 0.605659 1_4:-2164727 None:intergenic
AATGCATTAAGTATTGAGAC+CGG 0.605727 1_4:+2164316 Msa0143260:three_prime_UTR
GTTGTGGATTCAAGTGCTCG+TGG 0.610212 1_4:+2163290 Msa0143260:CDS
ATTTGAGGATCGATTTCGAG+AGG 0.616403 1_4:+2163130 Msa0143260:CDS
GCATATAGAAGATGTTGTTG+TGG 0.621571 1_4:+2163274 Msa0143260:CDS
GTGCTCGTGGGAAACAATTG+GGG 0.624260 1_4:+2163303 Msa0143260:CDS
TGGAAATGAAATCGGTAGTG+TGG 0.625238 1_4:+2164677 Msa0143260:three_prime_UTR
CGACAATATTTGTAGGACGG+AGG 0.628838 1_4:+2164418 Msa0143260:three_prime_UTR
CTGCGACAATATTTGTAGGA+CGG 0.635219 1_4:+2164415 Msa0143260:three_prime_UTR
GCATGAAAAGCAGTTCATAG+AGG 0.636091 1_4:+2164489 Msa0143260:three_prime_UTR
CTCAATCTAGTAAACCAGGA+AGG 0.641311 1_4:-2164723 None:intergenic
TTGTGGATTCAAGTGCTCGT+GGG 0.642750 1_4:+2163291 Msa0143260:CDS
AAGTTGATTAACATAGGACA+AGG 0.658019 1_4:+2164276 Msa0143260:three_prime_UTR
CTTGCAAACATCCACTACAA+TGG 0.659022 1_4:-2163795 None:intergenic
AAGGTGTTCTATGAGAAATG+TGG 0.671486 1_4:+2163407 Msa0143260:CDS
TTCGGTTGCACATAGCCAAG+AGG 0.685196 1_4:-2164172 None:intergenic
TTAGAAGATGCAAAACAGTG+AGG 0.719583 1_4:+2163004 None:intergenic
CCGGAAAGATTATTGCAACG+GGG 0.720016 1_4:+2163204 Msa0143260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGAAATTTTTAAGAAATTG+TGG + chr1_4:2163242-2163261 Msa0143260:CDS 15.0%
!!! ATTTTTTAAAGATTGATATC+AGG - chr1_4:2163852-2163871 None:intergenic 15.0%
!! AAATAAAAAAGGACTATATA+GGG - chr1_4:2164871-2164890 None:intergenic 15.0%
!! AAAATAAAAAAGGACTATAT+AGG - chr1_4:2164872-2164891 None:intergenic 15.0%
!! GATAGTTCAAAAAATAAAAA+AGG - chr1_4:2164882-2164901 None:intergenic 15.0%
!!! TTTGAACTATCTTATTATTT+TGG + chr1_4:2164891-2164910 Msa0143260:three_prime_UTR 15.0%
!!! AAGAAATTTTTAAGAAATTG+TGG + chr1_4:2163242-2163261 Msa0143260:CDS 15.0%
!!! ATTTTTTAAAGATTGATATC+AGG - chr1_4:2163852-2163871 None:intergenic 15.0%
!! AAATAAAAAAGGACTATATA+GGG - chr1_4:2164871-2164890 None:intergenic 15.0%
!! AAAATAAAAAAGGACTATAT+AGG - chr1_4:2164872-2164891 None:intergenic 15.0%
!! GATAGTTCAAAAAATAAAAA+AGG - chr1_4:2164882-2164901 None:intergenic 15.0%
!!! TTTGAACTATCTTATTATTT+TGG + chr1_4:2164891-2164910 Msa0143260:three_prime_UTR 15.0%
!! TAATCTTTCACTAAAAGAAA+TGG - chr1_4:2164000-2164019 None:intergenic 20.0%
!!! TTTGATAAGTTGATTAACAT+AGG + chr1_4:2164270-2164289 Msa0143260:three_prime_UTR 20.0%
!!! TTTTCTTAATTTGTGTCAAA+TGG + chr1_4:2164388-2164407 Msa0143260:three_prime_UTR 20.0%
!! TCCATTTATTACAAAAGTTA+AGG + chr1_4:2164525-2164544 Msa0143260:three_prime_UTR 20.0%
!!! TCCTTAACTTTTGTAATAAA+TGG - chr1_4:2164529-2164548 None:intergenic 20.0%
!! TTTATTACAAAAGTTAAGGA+AGG + chr1_4:2164529-2164548 Msa0143260:three_prime_UTR 20.0%
!! TAATCTTTCACTAAAAGAAA+TGG - chr1_4:2164000-2164019 None:intergenic 20.0%
!!! TTTGATAAGTTGATTAACAT+AGG + chr1_4:2164270-2164289 Msa0143260:three_prime_UTR 20.0%
!!! TTTTCTTAATTTGTGTCAAA+TGG + chr1_4:2164388-2164407 Msa0143260:three_prime_UTR 20.0%
!! TCCATTTATTACAAAAGTTA+AGG + chr1_4:2164525-2164544 Msa0143260:three_prime_UTR 20.0%
!!! TCCTTAACTTTTGTAATAAA+TGG - chr1_4:2164529-2164548 None:intergenic 20.0%
!! TTTATTACAAAAGTTAAGGA+AGG + chr1_4:2164529-2164548 Msa0143260:three_prime_UTR 20.0%
! TTAAGAAATTGTGGTAAAGT+TGG + chr1_4:2163251-2163270 Msa0143260:CDS 25.0%
! TAAGAAATTGTGGTAAAGTT+GGG + chr1_4:2163252-2163271 Msa0143260:CDS 25.0%
!!! TTCTTTTATTTCTCATGTGA+TGG + chr1_4:2163507-2163526 Msa0143260:intron 25.0%
!!! AAAGGCATTGAAAGAATAAA+AGG - chr1_4:2163751-2163770 None:intergenic 25.0%
! AACATTATTCGTAGTACATA+AGG - chr1_4:2163972-2163991 None:intergenic 25.0%
!! TCTGTTATTTACATGTGTTA+TGG + chr1_4:2164072-2164091 Msa0143260:three_prime_UTR 25.0%
!! TCTTATGGTACATTTGAAAT+GGG + chr1_4:2164640-2164659 Msa0143260:three_prime_UTR 25.0%
! TCCATGATTATGTCATTATA+CGG - chr1_4:2164773-2164792 None:intergenic 25.0%
! TCTTGATTCTTCTATTAAAC+AGG + chr1_4:2164802-2164821 Msa0143260:three_prime_UTR 25.0%
! TTAAGAAATTGTGGTAAAGT+TGG + chr1_4:2163251-2163270 Msa0143260:CDS 25.0%
! TAAGAAATTGTGGTAAAGTT+GGG + chr1_4:2163252-2163271 Msa0143260:CDS 25.0%
!!! TTCTTTTATTTCTCATGTGA+TGG + chr1_4:2163507-2163526 Msa0143260:intron 25.0%
!!! AAAGGCATTGAAAGAATAAA+AGG - chr1_4:2163751-2163770 None:intergenic 25.0%
! AACATTATTCGTAGTACATA+AGG - chr1_4:2163972-2163991 None:intergenic 25.0%
!! TCTGTTATTTACATGTGTTA+TGG + chr1_4:2164072-2164091 Msa0143260:three_prime_UTR 25.0%
!! TCTTATGGTACATTTGAAAT+GGG + chr1_4:2164640-2164659 Msa0143260:three_prime_UTR 25.0%
! TCCATGATTATGTCATTATA+CGG - chr1_4:2164773-2164792 None:intergenic 25.0%
! TCTTGATTCTTCTATTAAAC+AGG + chr1_4:2164802-2164821 Msa0143260:three_prime_UTR 25.0%
GAGATTACAGACAAGAAAAA+AGG + chr1_4:2163053-2163072 Msa0143260:CDS 30.0%
ATTCGCGAAAAGAAAAAACA+AGG - chr1_4:2163481-2163500 None:intergenic 30.0%
! ACGTTTTCTCAAATTATTGC+TGG + chr1_4:2163612-2163631 Msa0143260:intron 30.0%
AACTGCAAACAAACAACAAA+CGG - chr1_4:2163720-2163739 None:intergenic 30.0%
! CCATTGATGGTAGTTAATAA+AGG - chr1_4:2163769-2163788 None:intergenic 30.0%
CCTTTATTAACTACCATCAA+TGG + chr1_4:2163766-2163785 Msa0143260:CDS 30.0%
! AAGACATCTTGAAGTACTTA+CGG - chr1_4:2163911-2163930 None:intergenic 30.0%
AGTACTTCAAGATGTCTTTA+AGG + chr1_4:2163913-2163932 Msa0143260:three_prime_UTR 30.0%
GTCTTAAAAGCAGAAAAATG+AGG + chr1_4:2164210-2164229 Msa0143260:three_prime_UTR 30.0%
! AAGTTGATTAACATAGGACA+AGG + chr1_4:2164276-2164295 Msa0143260:three_prime_UTR 30.0%
!! AATGCATTAAGTATTGAGAC+CGG + chr1_4:2164316-2164335 Msa0143260:three_prime_UTR 30.0%
TCATGCCTATATAAAAACAG+AGG + chr1_4:2164455-2164474 Msa0143260:three_prime_UTR 30.0%
!!! TCCATCCTCTGTTTTTATAT+AGG - chr1_4:2164463-2164482 None:intergenic 30.0%
ATATAAAAACAGAGGATGGA+AGG + chr1_4:2164463-2164482 Msa0143260:three_prime_UTR 30.0%
AATTTGCCTGTTCAATGATT+TGG + chr1_4:2164558-2164577 Msa0143260:three_prime_UTR 30.0%
ATTTGCCTGTTCAATGATTT+GGG + chr1_4:2164559-2164578 Msa0143260:three_prime_UTR 30.0%
ATTCTTTGTTACAGCTCTTA+TGG + chr1_4:2164625-2164644 Msa0143260:three_prime_UTR 30.0%
CTCTTATGGTACATTTGAAA+TGG + chr1_4:2164639-2164658 Msa0143260:three_prime_UTR 30.0%
! ATGCTCTTTGGAAATGAAAT+CGG + chr1_4:2164669-2164688 Msa0143260:three_prime_UTR 30.0%
!! GTTTACTAGATTGAGTTGAT+GGG + chr1_4:2164731-2164750 Msa0143260:three_prime_UTR 30.0%
ACCGTATAATGACATAATCA+TGG + chr1_4:2164769-2164788 Msa0143260:three_prime_UTR 30.0%
ATTCTTCTATTAAACAGGTG+TGG + chr1_4:2164807-2164826 Msa0143260:three_prime_UTR 30.0%
GAGATTACAGACAAGAAAAA+AGG + chr1_4:2163053-2163072 Msa0143260:CDS 30.0%
ATTCGCGAAAAGAAAAAACA+AGG - chr1_4:2163481-2163500 None:intergenic 30.0%
! ACGTTTTCTCAAATTATTGC+TGG + chr1_4:2163612-2163631 Msa0143260:intron 30.0%
AACTGCAAACAAACAACAAA+CGG - chr1_4:2163720-2163739 None:intergenic 30.0%
! CCATTGATGGTAGTTAATAA+AGG - chr1_4:2163769-2163788 None:intergenic 30.0%
CCTTTATTAACTACCATCAA+TGG + chr1_4:2163766-2163785 Msa0143260:CDS 30.0%
! AAGACATCTTGAAGTACTTA+CGG - chr1_4:2163911-2163930 None:intergenic 30.0%
AGTACTTCAAGATGTCTTTA+AGG + chr1_4:2163913-2163932 Msa0143260:three_prime_UTR 30.0%
GTCTTAAAAGCAGAAAAATG+AGG + chr1_4:2164210-2164229 Msa0143260:three_prime_UTR 30.0%
! AAGTTGATTAACATAGGACA+AGG + chr1_4:2164276-2164295 Msa0143260:three_prime_UTR 30.0%
!! AATGCATTAAGTATTGAGAC+CGG + chr1_4:2164316-2164335 Msa0143260:three_prime_UTR 30.0%
TCATGCCTATATAAAAACAG+AGG + chr1_4:2164455-2164474 Msa0143260:three_prime_UTR 30.0%
!!! TCCATCCTCTGTTTTTATAT+AGG - chr1_4:2164463-2164482 None:intergenic 30.0%
ATATAAAAACAGAGGATGGA+AGG + chr1_4:2164463-2164482 Msa0143260:three_prime_UTR 30.0%
AATTTGCCTGTTCAATGATT+TGG + chr1_4:2164558-2164577 Msa0143260:three_prime_UTR 30.0%
ATTTGCCTGTTCAATGATTT+GGG + chr1_4:2164559-2164578 Msa0143260:three_prime_UTR 30.0%
ATTCTTTGTTACAGCTCTTA+TGG + chr1_4:2164625-2164644 Msa0143260:three_prime_UTR 30.0%
CTCTTATGGTACATTTGAAA+TGG + chr1_4:2164639-2164658 Msa0143260:three_prime_UTR 30.0%
! ATGCTCTTTGGAAATGAAAT+CGG + chr1_4:2164669-2164688 Msa0143260:three_prime_UTR 30.0%
!! GTTTACTAGATTGAGTTGAT+GGG + chr1_4:2164731-2164750 Msa0143260:three_prime_UTR 30.0%
ACCGTATAATGACATAATCA+TGG + chr1_4:2164769-2164788 Msa0143260:three_prime_UTR 30.0%
ATTCTTCTATTAAACAGGTG+TGG + chr1_4:2164807-2164826 Msa0143260:three_prime_UTR 30.0%
GTCTGTAATCTCTAATCTTC+TGG - chr1_4:2163045-2163064 None:intergenic 35.0%
TTGTGATTCAGTCTCTGATA+AGG + chr1_4:2163106-2163125 Msa0143260:CDS 35.0%
AGTCTCTGATAAGGAATTTG+AGG + chr1_4:2163115-2163134 Msa0143260:CDS 35.0%
GTTATTGAAGATGAGATTGC+CGG + chr1_4:2163185-2163204 Msa0143260:CDS 35.0%
GCATATAGAAGATGTTGTTG+TGG + chr1_4:2163274-2163293 Msa0143260:CDS 35.0%
! AAGGTGTTCTATGAGAAATG+TGG + chr1_4:2163407-2163426 Msa0143260:CDS 35.0%
CTATGAGAAATGTGGTTTCA+AGG + chr1_4:2163415-2163434 Msa0143260:CDS 35.0%
TAAACTACATGTGTCATGTC+CGG - chr1_4:2163573-2163592 None:intergenic 35.0%
GATAGTATCAATTGCTGAAG+AGG - chr1_4:2163688-2163707 None:intergenic 35.0%
! GTTGTGCATAGTGTTCATTT+TGG + chr1_4:2163877-2163896 Msa0143260:three_prime_UTR 35.0%
! CTTGAAGTACTTACGGAAAT+AGG - chr1_4:2163904-2163923 None:intergenic 35.0%
GACTAAACAAGCACATGTTT+CGG - chr1_4:2164193-2164212 None:intergenic 35.0%
ATCAAAAACTACAGCTGAAC+TGG - chr1_4:2164256-2164275 None:intergenic 35.0%
ATAGGACAAGGTTGTCTTTA+TGG + chr1_4:2164288-2164307 Msa0143260:three_prime_UTR 35.0%
TCAACTGCGACAATATTTGT+AGG + chr1_4:2164411-2164430 Msa0143260:three_prime_UTR 35.0%
GCCTATATAAAAACAGAGGA+TGG + chr1_4:2164459-2164478 Msa0143260:three_prime_UTR 35.0%
! TTCAATGATTTGGGTGCTTA+AGG + chr1_4:2164568-2164587 Msa0143260:three_prime_UTR 35.0%
GAATTATGAGGACAAGCATA+TGG + chr1_4:2164590-2164609 Msa0143260:three_prime_UTR 35.0%
AGCTGTAACAAAGAATTTCG+AGG - chr1_4:2164621-2164640 None:intergenic 35.0%
!! TTCATTTCCAAAGAGCATCA+AGG - chr1_4:2164667-2164686 None:intergenic 35.0%
TCAACTCAATCTAGTAAACC+AGG - chr1_4:2164730-2164749 None:intergenic 35.0%
!! GGTTTACTAGATTGAGTTGA+TGG + chr1_4:2164730-2164749 Msa0143260:three_prime_UTR 35.0%
TTCTATTAAACAGGTGTGGT+GGG + chr1_4:2164811-2164830 Msa0143260:three_prime_UTR 35.0%
GTCTGTAATCTCTAATCTTC+TGG - chr1_4:2163045-2163064 None:intergenic 35.0%
TTGTGATTCAGTCTCTGATA+AGG + chr1_4:2163106-2163125 Msa0143260:CDS 35.0%
AGTCTCTGATAAGGAATTTG+AGG + chr1_4:2163115-2163134 Msa0143260:CDS 35.0%
GTTATTGAAGATGAGATTGC+CGG + chr1_4:2163185-2163204 Msa0143260:CDS 35.0%
GCATATAGAAGATGTTGTTG+TGG + chr1_4:2163274-2163293 Msa0143260:CDS 35.0%
! AAGGTGTTCTATGAGAAATG+TGG + chr1_4:2163407-2163426 Msa0143260:CDS 35.0%
CTATGAGAAATGTGGTTTCA+AGG + chr1_4:2163415-2163434 Msa0143260:CDS 35.0%
TAAACTACATGTGTCATGTC+CGG - chr1_4:2163573-2163592 None:intergenic 35.0%
GATAGTATCAATTGCTGAAG+AGG - chr1_4:2163688-2163707 None:intergenic 35.0%
! GTTGTGCATAGTGTTCATTT+TGG + chr1_4:2163877-2163896 Msa0143260:three_prime_UTR 35.0%
! CTTGAAGTACTTACGGAAAT+AGG - chr1_4:2163904-2163923 None:intergenic 35.0%
GACTAAACAAGCACATGTTT+CGG - chr1_4:2164193-2164212 None:intergenic 35.0%
ATCAAAAACTACAGCTGAAC+TGG - chr1_4:2164256-2164275 None:intergenic 35.0%
ATAGGACAAGGTTGTCTTTA+TGG + chr1_4:2164288-2164307 Msa0143260:three_prime_UTR 35.0%
TCAACTGCGACAATATTTGT+AGG + chr1_4:2164411-2164430 Msa0143260:three_prime_UTR 35.0%
GCCTATATAAAAACAGAGGA+TGG + chr1_4:2164459-2164478 Msa0143260:three_prime_UTR 35.0%
! TTCAATGATTTGGGTGCTTA+AGG + chr1_4:2164568-2164587 Msa0143260:three_prime_UTR 35.0%
GAATTATGAGGACAAGCATA+TGG + chr1_4:2164590-2164609 Msa0143260:three_prime_UTR 35.0%
AGCTGTAACAAAGAATTTCG+AGG - chr1_4:2164621-2164640 None:intergenic 35.0%
!! TTCATTTCCAAAGAGCATCA+AGG - chr1_4:2164667-2164686 None:intergenic 35.0%
TCAACTCAATCTAGTAAACC+AGG - chr1_4:2164730-2164749 None:intergenic 35.0%
!! GGTTTACTAGATTGAGTTGA+TGG + chr1_4:2164730-2164749 Msa0143260:three_prime_UTR 35.0%
TTCTATTAAACAGGTGTGGT+GGG + chr1_4:2164811-2164830 Msa0143260:three_prime_UTR 35.0%
! ATTTGAGGATCGATTTCGAG+AGG + chr1_4:2163130-2163149 Msa0143260:CDS 40.0%
! TTTCGAGAGGTTGATTCACT+TGG + chr1_4:2163143-2163162 Msa0143260:CDS 40.0%
TGCCGGAAAGATTATTGCAA+CGG + chr1_4:2163202-2163221 Msa0143260:CDS 40.0%
! TCGTTCTGTTGGGTGTTATA+AGG + chr1_4:2163358-2163377 Msa0143260:CDS 40.0%
CAAGGAGAAAGAAGTTCAGA+TGG + chr1_4:2163433-2163452 Msa0143260:CDS 40.0%
! GGTTCTAGTCAGATTGAACA+TGG + chr1_4:2163528-2163547 Msa0143260:intron 40.0%
CAAACATCCACTACAATGGT+AGG - chr1_4:2163794-2163813 None:intergenic 40.0%
CTTGCAAACATCCACTACAA+TGG - chr1_4:2163798-2163817 None:intergenic 40.0%
! GTCATAGAACTCTAGAAAGC+AGG - chr1_4:2163826-2163845 None:intergenic 40.0%
ATGAACACTATGCACAACTC+TGG - chr1_4:2163875-2163894 None:intergenic 40.0%
GTACATAAGGCGATGTATGA+TGG - chr1_4:2163959-2163978 None:intergenic 40.0%
CTCACTGTTATCTGTACTCT+TGG + chr1_4:2164135-2164154 Msa0143260:three_prime_UTR 40.0%
AAAAACTACAGCTGAACTGG+TGG - chr1_4:2164253-2164272 None:intergenic 40.0%
GATCAGAATTACAGCTGAAC+CGG - chr1_4:2164338-2164357 None:intergenic 40.0%
CTGCGACAATATTTGTAGGA+CGG + chr1_4:2164415-2164434 Msa0143260:three_prime_UTR 40.0%
GACAATATTTGTAGGACGGA+GGG + chr1_4:2164419-2164438 Msa0143260:three_prime_UTR 40.0%
AAAAACAGAGGATGGAAGGA+TGG + chr1_4:2164467-2164486 Msa0143260:three_prime_UTR 40.0%
GCATGAAAAGCAGTTCATAG+AGG + chr1_4:2164489-2164508 Msa0143260:three_prime_UTR 40.0%
AAGCACCCAAATCATTGAAC+AGG - chr1_4:2164567-2164586 None:intergenic 40.0%
TGGGTGCTTAAGGAATTATG+AGG + chr1_4:2164578-2164597 Msa0143260:three_prime_UTR 40.0%
TGGAAATGAAATCGGTAGTG+TGG + chr1_4:2164677-2164696 Msa0143260:three_prime_UTR 40.0%
AATGAAATCGGTAGTGTGGT+AGG + chr1_4:2164681-2164700 Msa0143260:three_prime_UTR 40.0%
CTCAATCTAGTAAACCAGGA+AGG - chr1_4:2164726-2164745 None:intergenic 40.0%
CTTCTATTAAACAGGTGTGG+TGG + chr1_4:2164810-2164829 Msa0143260:three_prime_UTR 40.0%
! ATTTGAGGATCGATTTCGAG+AGG + chr1_4:2163130-2163149 Msa0143260:CDS 40.0%
! TTTCGAGAGGTTGATTCACT+TGG + chr1_4:2163143-2163162 Msa0143260:CDS 40.0%
TGCCGGAAAGATTATTGCAA+CGG + chr1_4:2163202-2163221 Msa0143260:CDS 40.0%
! TCGTTCTGTTGGGTGTTATA+AGG + chr1_4:2163358-2163377 Msa0143260:CDS 40.0%
CAAGGAGAAAGAAGTTCAGA+TGG + chr1_4:2163433-2163452 Msa0143260:CDS 40.0%
! GGTTCTAGTCAGATTGAACA+TGG + chr1_4:2163528-2163547 Msa0143260:intron 40.0%
CAAACATCCACTACAATGGT+AGG - chr1_4:2163794-2163813 None:intergenic 40.0%
CTTGCAAACATCCACTACAA+TGG - chr1_4:2163798-2163817 None:intergenic 40.0%
! GTCATAGAACTCTAGAAAGC+AGG - chr1_4:2163826-2163845 None:intergenic 40.0%
ATGAACACTATGCACAACTC+TGG - chr1_4:2163875-2163894 None:intergenic 40.0%
GTACATAAGGCGATGTATGA+TGG - chr1_4:2163959-2163978 None:intergenic 40.0%
CTCACTGTTATCTGTACTCT+TGG + chr1_4:2164135-2164154 Msa0143260:three_prime_UTR 40.0%
AAAAACTACAGCTGAACTGG+TGG - chr1_4:2164253-2164272 None:intergenic 40.0%
GATCAGAATTACAGCTGAAC+CGG - chr1_4:2164338-2164357 None:intergenic 40.0%
CTGCGACAATATTTGTAGGA+CGG + chr1_4:2164415-2164434 Msa0143260:three_prime_UTR 40.0%
GACAATATTTGTAGGACGGA+GGG + chr1_4:2164419-2164438 Msa0143260:three_prime_UTR 40.0%
AAAAACAGAGGATGGAAGGA+TGG + chr1_4:2164467-2164486 Msa0143260:three_prime_UTR 40.0%
GCATGAAAAGCAGTTCATAG+AGG + chr1_4:2164489-2164508 Msa0143260:three_prime_UTR 40.0%
AAGCACCCAAATCATTGAAC+AGG - chr1_4:2164567-2164586 None:intergenic 40.0%
TGGGTGCTTAAGGAATTATG+AGG + chr1_4:2164578-2164597 Msa0143260:three_prime_UTR 40.0%
TGGAAATGAAATCGGTAGTG+TGG + chr1_4:2164677-2164696 Msa0143260:three_prime_UTR 40.0%
AATGAAATCGGTAGTGTGGT+AGG + chr1_4:2164681-2164700 Msa0143260:three_prime_UTR 40.0%
CTCAATCTAGTAAACCAGGA+AGG - chr1_4:2164726-2164745 None:intergenic 40.0%
CTTCTATTAAACAGGTGTGG+TGG + chr1_4:2164810-2164829 Msa0143260:three_prime_UTR 40.0%
GCCGGAAAGATTATTGCAAC+GGG + chr1_4:2163203-2163222 Msa0143260:CDS 45.0%
CCCCGTTGCAATAATCTTTC+CGG - chr1_4:2163207-2163226 None:intergenic 45.0%
CCGGAAAGATTATTGCAACG+GGG + chr1_4:2163204-2163223 Msa0143260:CDS 45.0%
TTGTGGATTCAAGTGCTCGT+GGG + chr1_4:2163291-2163310 Msa0143260:CDS 45.0%
AAGTGCTCGTGGGAAACAAT+TGG + chr1_4:2163301-2163320 Msa0143260:CDS 45.0%
AGTGCTCGTGGGAAACAATT+GGG + chr1_4:2163302-2163321 Msa0143260:CDS 45.0%
! CGATTGCAGTGTTGAGAACA+AGG + chr1_4:2163388-2163407 Msa0143260:CDS 45.0%
GTCAGATTGAACATGGTGTC+TGG + chr1_4:2163535-2163554 Msa0143260:intron 45.0%
TGTCTGGCAAATGTCAATGC+CGG + chr1_4:2163551-2163570 Msa0143260:intron 45.0%
ACAATGGTAGGAGCCATTGA+TGG - chr1_4:2163782-2163801 None:intergenic 45.0%
AATGGCTCCTACCATTGTAG+TGG + chr1_4:2163784-2163803 Msa0143260:CDS 45.0%
! AACTGGTGGCGTCAATATGT+GGG - chr1_4:2164239-2164258 None:intergenic 45.0%
CGACAATATTTGTAGGACGG+AGG + chr1_4:2164418-2164437 Msa0143260:three_prime_UTR 45.0%
AATGGGACCTTGATGCTCTT+TGG + chr1_4:2164657-2164676 Msa0143260:three_prime_UTR 45.0%
!! ATTAAACAGGTGTGGTGGGT+TGG + chr1_4:2164815-2164834 Msa0143260:three_prime_UTR 45.0%
!! TGGTGGGTTGGACATTATTC+TGG + chr1_4:2164827-2164846 Msa0143260:three_prime_UTR 45.0%
GCCGGAAAGATTATTGCAAC+GGG + chr1_4:2163203-2163222 Msa0143260:CDS 45.0%
CCCCGTTGCAATAATCTTTC+CGG - chr1_4:2163207-2163226 None:intergenic 45.0%
CCGGAAAGATTATTGCAACG+GGG + chr1_4:2163204-2163223 Msa0143260:CDS 45.0%
TTGTGGATTCAAGTGCTCGT+GGG + chr1_4:2163291-2163310 Msa0143260:CDS 45.0%
AAGTGCTCGTGGGAAACAAT+TGG + chr1_4:2163301-2163320 Msa0143260:CDS 45.0%
AGTGCTCGTGGGAAACAATT+GGG + chr1_4:2163302-2163321 Msa0143260:CDS 45.0%
! CGATTGCAGTGTTGAGAACA+AGG + chr1_4:2163388-2163407 Msa0143260:CDS 45.0%
GTCAGATTGAACATGGTGTC+TGG + chr1_4:2163535-2163554 Msa0143260:intron 45.0%
TGTCTGGCAAATGTCAATGC+CGG + chr1_4:2163551-2163570 Msa0143260:intron 45.0%
ACAATGGTAGGAGCCATTGA+TGG - chr1_4:2163782-2163801 None:intergenic 45.0%
AATGGCTCCTACCATTGTAG+TGG + chr1_4:2163784-2163803 Msa0143260:CDS 45.0%
! AACTGGTGGCGTCAATATGT+GGG - chr1_4:2164239-2164258 None:intergenic 45.0%
CGACAATATTTGTAGGACGG+AGG + chr1_4:2164418-2164437 Msa0143260:three_prime_UTR 45.0%
AATGGGACCTTGATGCTCTT+TGG + chr1_4:2164657-2164676 Msa0143260:three_prime_UTR 45.0%
!! ATTAAACAGGTGTGGTGGGT+TGG + chr1_4:2164815-2164834 Msa0143260:three_prime_UTR 45.0%
!! TGGTGGGTTGGACATTATTC+TGG + chr1_4:2164827-2164846 Msa0143260:three_prime_UTR 45.0%
GTTGTGGATTCAAGTGCTCG+TGG + chr1_4:2163290-2163309 Msa0143260:CDS 50.0%
GTGCTCGTGGGAAACAATTG+GGG + chr1_4:2163303-2163322 Msa0143260:CDS 50.0%
ACCGATCATGCTCGTTCTGT+TGG + chr1_4:2163347-2163366 Msa0143260:CDS 50.0%
CCCAACAGAACGAGCATGAT+CGG - chr1_4:2163351-2163370 None:intergenic 50.0%
CCGATCATGCTCGTTCTGTT+GGG + chr1_4:2163348-2163367 Msa0143260:CDS 50.0%
GCTATTGTAGCCTGACCTCT+TGG + chr1_4:2164157-2164176 Msa0143260:three_prime_UTR 50.0%
TTGCACATAGCCAAGAGGTC+AGG - chr1_4:2164170-2164189 None:intergenic 50.0%
TTCGGTTGCACATAGCCAAG+AGG - chr1_4:2164175-2164194 None:intergenic 50.0%
! GAACTGGTGGCGTCAATATG+TGG - chr1_4:2164240-2164259 None:intergenic 50.0%
CAGCAGTACATGTTCCTTCC+TGG + chr1_4:2164709-2164728 Msa0143260:three_prime_UTR 50.0%
GTTGTGGATTCAAGTGCTCG+TGG + chr1_4:2163290-2163309 Msa0143260:CDS 50.0%
GTGCTCGTGGGAAACAATTG+GGG + chr1_4:2163303-2163322 Msa0143260:CDS 50.0%
ACCGATCATGCTCGTTCTGT+TGG + chr1_4:2163347-2163366 Msa0143260:CDS 50.0%
CCCAACAGAACGAGCATGAT+CGG - chr1_4:2163351-2163370 None:intergenic 50.0%
CCGATCATGCTCGTTCTGTT+GGG + chr1_4:2163348-2163367 Msa0143260:CDS 50.0%
GCTATTGTAGCCTGACCTCT+TGG + chr1_4:2164157-2164176 Msa0143260:three_prime_UTR 50.0%
TTGCACATAGCCAAGAGGTC+AGG - chr1_4:2164170-2164189 None:intergenic 50.0%
TTCGGTTGCACATAGCCAAG+AGG - chr1_4:2164175-2164194 None:intergenic 50.0%
! GAACTGGTGGCGTCAATATG+TGG - chr1_4:2164240-2164259 None:intergenic 50.0%
CAGCAGTACATGTTCCTTCC+TGG + chr1_4:2164709-2164728 Msa0143260:three_prime_UTR 50.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 2163011 2164931 2163011 ID=Msa0143260;Name=Msa0143260
chr1_4 mRNA 2163011 2164931 2163011 ID=Msa0143260-mRNA-1;Parent=Msa0143260;Name=Msa0143260-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0.5|1|1|1|2|1097|181
chr1_4 exon 2163011 2163459 2163011 ID=Msa0143260-mRNA-1:exon:827;Parent=Msa0143260-mRNA-1
chr1_4 exon 2163738 2164931 2163738 ID=Msa0143260-mRNA-1:exon:828;Parent=Msa0143260-mRNA-1
chr1_4 CDS 2163011 2163459 2163011 ID=Msa0143260-mRNA-1:cds;Parent=Msa0143260-mRNA-1
chr1_4 CDS 2163738 2163834 2163738 ID=Msa0143260-mRNA-1:cds;Parent=Msa0143260-mRNA-1
chr1_4 three_prime_UTR 2163835 2164931 2163835 ID=Msa0143260-mRNA-1:three_prime_utr;Parent=Msa0143260-mRNA-1
Gene Sequence

>Msa0143260

ATGCAAAACAGTGAGGAACAAAAGTATAGAGCCAGAAGATTAGAGATTACAGACAAGAAAAAAGGTTTCATAGAATTACTACAACAGCTTAGCGTTTGTGATTCAGTCTCTGATAAGGAATTTGAGGATCGATTTCGAGAGGTTGATTCACTTGGAGATGATCATGTTATATGTGTTATTGAAGATGAGATTGCCGGAAAGATTATTGCAACGGGGAGTGTGTTTATTGAGAAGAAATTTTTAAGAAATTGTGGTAAAGTTGGGCATATAGAAGATGTTGTTGTGGATTCAAGTGCTCGTGGGAAACAATTGGGGAAGAAAGTAATTAACTTTCTTACCGATCATGCTCGTTCTGTTGGGTGTTATAAGGTAATTCTCGATTGCAGTGTTGAGAACAAGGTGTTCTATGAGAAATGTGGTTTCAAGGAGAAAGAAGTTCAGATGGCTATTTATATAATTCCTTTTATTCTTTCAATGCCTTTATTAACTACCATCAATGGCTCCTACCATTGTAGTGGATGTTTGCAAGAGCAACCTGCTTTCTAG
Protein sequence

>Msa0143260

MQNSEEQKYRARRLEITDKKKGFIELLQQLSVCDSVSDKEFEDRFREVDSLGDDHVICVIEDEIAGKIIATGSVFIEKKFLRNCGKVGHIEDVVVDSSARGKQLGKKVINFLTDHARSVGCYKVILDCSVENKVFYEKCGFKEKEVQMAIYIIPFILSMPLLTTINGSYHCSGCLQEQPAF*