AlfalfaGEDB | Msa0144060

M. sativa cultivar zhongmu-4 / Msa0144060

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0144060	KEH40807.1	82.111	341	29	4	1	311	1	339	0.0	555

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0144060	sp\|Q9XIB4\|Y1475_ARATH	29.932	147	88	6	9	152	34	168	6.42e-11	63.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0144060	A0A072VG97	82.111	341	29	4	1	311	1	339	0.0	555

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TFs/TRs:

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Gene ID	Type	Classification
Msa0144060	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0144060	MtrunA17_Chr1g0163231	90.845	284	24	2	1	284	1	282	0.0	531
Msa0144060	MtrunA17_Chr1g0171611	87.368	285	20	2	1	284	1	270	1.67e-176	489
Msa0144060	MtrunA17_Chr1g0163261	57.333	300	100	9	1	285	1	287	1.84e-104	307
Msa0144060	MtrunA17_Chr1g0163251	51.890	291	121	7	1	282	1	281	7.73e-95	286
Msa0144060	MtrunA17_Chr1g0163201	47.134	314	131	9	24	311	18	322	1.29e-83	258
Msa0144060	MtrunA17_Chr1g0163181	46.557	305	131	8	1	283	1	295	5.28e-83	257
Msa0144060	MtrunA17_Chr1g0163241	60.150	133	41	4	24	149	22	149	8.38e-47	155
Msa0144060	MtrunA17_Chr1g0163191	55.285	123	51	1	1	123	1	119	1.21e-41	142
Msa0144060	MtrunA17_Chr3g0131891	31.615	291	177	9	6	279	16	301	1.54e-33	125
Msa0144060	MtrunA17_Chr7g0233561	31.313	297	180	8	6	279	15	310	2.54e-33	125

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0144060	AT1G49475.1	29.932	147	88	6	9	152	34	168	6.52e-12	63.9
Msa0144060	AT3G18990.1	22.849	337	184	10	9	278	6	333	5.57e-11	63.2
Msa0144060	AT3G18990.2	22.619	336	186	9	9	278	6	333	6.47e-11	63.2

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Find 51 sgRNAs with CRISPR-Local

Find 204 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCTTAACCTTAAAATA+TGG	0.178478	1_4:+3505697	None:intergenic
AAATCTTAACCTTAAAATAT+GGG	0.446492	1_4:+3505698	None:intergenic
AACATCACCTTCACTTTCAT+AGG	0.384516	1_4:+3505028	None:intergenic
ACACGAATCTCCATTGTGAT+CGG	0.544622	1_4:+3504243	None:intergenic
ACTATCTTCAAGAACTCTTC+TGG	0.329140	1_4:+3506140	None:intergenic
ACTTACCAAAGTATAGCCAT+GGG	0.659069	1_4:+3505291	None:intergenic
ACTTCCAAATGGTGCTCAAA+AGG	0.444677	1_4:-3505830	Msa0144060:CDS
AGAAATTTGTGGAGAAATAT+TGG	0.241294	1_4:-3505882	Msa0144060:CDS
AGAACTTGCATTGTGACCAG+TGG	0.689771	1_4:-3504986	Msa0144060:CDS
AGCTCAAGCAAGAGACTTAG+AGG	0.407272	1_4:+3504097	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCGAAATCATGAACTACATA+AGG	-	chr1_4:3504138-3504157	Msa0144060:intron	30.0%
CGAAATCATGAACTACATAA+GGG	-	chr1_4:3504139-3504158	Msa0144060:intron	30.0%
ATAAGGATATGTAATGCAAC+AGG	+	chr1_4:3504317-3504336	None:intergenic	30.0%
AGTCTAAGAATTACTGGATT+TGG	+	chr1_4:3504408-3504427	None:intergenic	30.0%
TTTGGAAGTCTAAGAATTAC+TGG	+	chr1_4:3504414-3504433	None:intergenic	30.0%
GTAATTCTTAGACTTCCAAA+TGG	-	chr1_4:3504414-3504433	Msa0144060:intron	30.0%
TTCATTGATGAAAGTTGTGA+AGG	-	chr1_4:3504621-3504640	Msa0144060:intron	30.0%
ACATCTCAAAGGAGAAAAAA+CGG	-	chr1_4:3504705-3504724	Msa0144060:intron	30.0%
GTATCCTCTATTTCAATTCA+AGG	+	chr1_4:3505130-3505149	None:intergenic	30.0%
ATAGTTATGGTGAATGAAAG+TGG	+	chr1_4:3505368-3505387	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	3504100	3506177	3504100	ID=Msa0144060;Name=Msa0144060
chr1_4	mRNA	3504100	3506177	3504100	ID=Msa0144060-mRNA-1;Parent=Msa0144060;Name=Msa0144060-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0\|0\|0\|0.66\|1\|1\|6\|0\|319
chr1_4	exon	3506109	3506177	3506109	ID=Msa0144060-mRNA-1:exon:1442;Parent=Msa0144060-mRNA-1
chr1_4	exon	3505518	3505914	3505518	ID=Msa0144060-mRNA-1:exon:1441;Parent=Msa0144060-mRNA-1
chr1_4	exon	3505297	3505421	3505297	ID=Msa0144060-mRNA-1:exon:1440;Parent=Msa0144060-mRNA-1
chr1_4	exon	3504876	3505134	3504876	ID=Msa0144060-mRNA-1:exon:1439;Parent=Msa0144060-mRNA-1
chr1_4	exon	3504227	3504291	3504227	ID=Msa0144060-mRNA-1:exon:1438;Parent=Msa0144060-mRNA-1
chr1_4	exon	3504100	3504144	3504100	ID=Msa0144060-mRNA-1:exon:1437;Parent=Msa0144060-mRNA-1
chr1_4	CDS	3506109	3506177	3506109	ID=Msa0144060-mRNA-1:cds;Parent=Msa0144060-mRNA-1
chr1_4	CDS	3505518	3505914	3505518	ID=Msa0144060-mRNA-1:cds;Parent=Msa0144060-mRNA-1
chr1_4	CDS	3505297	3505421	3505297	ID=Msa0144060-mRNA-1:cds;Parent=Msa0144060-mRNA-1
chr1_4	CDS	3504876	3505134	3504876	ID=Msa0144060-mRNA-1:cds;Parent=Msa0144060-mRNA-1
chr1_4	CDS	3504227	3504291	3504227	ID=Msa0144060-mRNA-1:cds;Parent=Msa0144060-mRNA-1
chr1_4	CDS	3504100	3504144	3504100	ID=Msa0144060-mRNA-1:cds;Parent=Msa0144060-mRNA-1

Gene Sequence

>Msa0144060

ATGTCATTACATCATCCAGAAGAGTTCTTGAAGATAGTTCGAAATCATGAACTACATAAGGGAGAGCTTAGGGTTCCCAAGAAATTTGTGGAGAAATATTGGAAAAAAATACCAAATCCAGTAATTCTTAGACTTCCAAATGGTGCTCAAAAGGAAATATTTTGGGTGGAGCGTGAAGGTGATATTTGGTTTAAGAGAAATTGGGAGAATTTTGCAAAGTCTCTAAAAGATGGATATGCTTTAGTATTCAAATATTTAGGTGGCCCATATTTTAAGGTTAAGATTTTTGGTCACAATACATTGGAAAAAGACTATTCCAATATAAAATTCATTGATGAAAGTTGTGAAGGTAGAGAAGAAGTTCAAGAAGTTGTTGAACTGAGTGATGACAATGATACTAATAGTGCTGGCACATCTCAAAGGAGAAAAAACGGCAAGAGAAAAAATATTCAGCAAAAAATCACAGGTAGTAAGAGAGGAGGTATGATTAAGAAGGCTAAAAAATGCTCAACAATTAGTGAGAGAGACAATAATGAGAATCCAAGTTTTGAACTTGAATTGTCACAATCTTACGCCCATGGCTATACTTTGAGGATACCAAGTGACTTTTCAAGAGCATATTTGAATGAGAACCTTAAAGGGTCATCTGCAAGCATTAGATTTGGTGAGAACATGCCTATGAAAGTGAAGGTGATGTTTCAAGATATCAAGAATAATAGAACTTGCATTGTGACCAGTGGTTGGAAGCCATTTGCCAAAAAATATAACTTACAAGTTGGTGATAAATGTAAATTTGTGATGACTCAACTTCAACCACTTTCATTCACCATAACTATTACCCAAGCCAAAGCAGACTTAGGTGGTAATGTGATGAAACCGATCACAATGGAGATTCGTGTGAATTCTACTTACCCGTATATTCGACATGAGTTTAAAATGCCCTCTAAGTCTCTTGCTTGA

Protein sequence

>Msa0144060

MSLHHPEEFLKIVRNHELHKGELRVPKKFVEKYWKKIPNPVILRLPNGAQKEIFWVEREGDIWFKRNWENFAKSLKDGYALVFKYLGGPYFKVKIFGHNTLEKDYSNIKFIDESCEGREEVQEVVELSDDNDTNSAGTSQRRKNGKRKNIQQKITGSKRGGMIKKAKKCSTISERDNNENPSFELELSQSYAHGYTLRIPSDFSRAYLNENLKGSSASIRFGENMPMKVKVMFQDIKNNRTCIVTSGWKPFAKKYNLQVGDKCKFVMTQLQPLSFTITITQAKADLGGNVMKPITMEIRVNSTYPYIRHEFKMPSKSLA*