AlfalfaGEDB | Msa0145210

M. sativa cultivar zhongmu-4 / Msa0145210

KEGG: K03141
GO: GO:0000428 GO:0000439 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005675 GO:0006139 GO:0006259 GO:0006281 GO:0006351 GO:0006355 GO:0006357 GO:0006360 GO:0006366 GO:0006464 GO:0006468 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009889 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016310 GO:0016591 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0030880 GO:0031323 GO:0031326 GO:0031974 GO:0031981 GO:0032774 GO:0032806 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0044798 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0055029 GO:0060255 GO:0061695 GO:0065007 GO:0070013 GO:0070816 GO:0071704 GO:0080090 GO:0090304 GO:0090575 GO:0097659 GO:1901360 GO:1901362 GO:1901564 GO:1901576 GO:1902494 GO:1902554 GO:1902911 GO:1903506 GO:1990234 GO:2000112 GO:2001141
PFAM: PF08567.14

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0145210	sp\|Q3ECP0\|TFB1A_ARATH	52.885	104	46	1	1	104	1	101	4.84e-30	119
Msa0145210	sp\|Q3ECP0\|TFB1A_ARATH	61.250	80	31	0	104	183	168	247	2.40e-26	108

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0145210	A0A2P5FYM5	58.883	197	62	2	5	182	2	198	2.35e-72	238

TFs/TRs:

Gene ID	Type	Classification
Msa0145210	TF	BSD

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0145210	MtrunA17_Chr6g0479371	51.837	245	51	7	3	182	2	244	4.75e-64	207
Msa0145210	MtrunA17_Chr1g0196731	62.048	166	48	4	3	168	2	152	1.35e-58	193
Msa0145210	MtrunA17_Chr1g0196731	92.500	80	6	0	103	182	164	243	8.14e-46	159

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0145210	AT1G55750.4	52.885	104	46	1	1	104	1	101	3.17e-31	119
Msa0145210	AT1G55750.4	61.250	80	31	0	104	183	168	247	1.64e-27	108
Msa0145210	AT1G55750.2	52.885	104	46	1	1	104	1	101	3.17e-31	119
Msa0145210	AT1G55750.2	61.250	80	31	0	104	183	168	247	1.64e-27	108
Msa0145210	AT1G55750.1	52.885	104	46	1	1	104	1	101	4.92e-31	119
Msa0145210	AT1G55750.1	61.250	80	31	0	104	183	168	247	2.44e-27	108
Msa0145210	AT1G55750.6	52.885	104	46	1	1	104	1	101	4.92e-31	119
Msa0145210	AT1G55750.6	61.250	80	31	0	104	183	168	247	2.44e-27	108
Msa0145210	AT1G55750.3	52.885	104	46	1	1	104	1	101	5.04e-31	119
Msa0145210	AT1G55750.3	61.250	80	31	0	104	183	168	247	2.95e-27	108
Msa0145210	AT1G55750.5	52.885	104	46	1	1	104	1	101	5.04e-31	119
Msa0145210	AT1G55750.5	61.250	80	31	0	104	183	168	247	2.95e-27	108
Msa0145210	AT3G61420.1	41.509	159	81	4	12	169	4	151	1.16e-29	115
Msa0145210	AT3G61420.1	60.000	80	32	0	104	183	163	242	4.72e-26	105
Msa0145210	AT3G61420.2	60.000	80	32	0	104	183	138	217	4.88e-26	104
Msa0145210	AT3G61420.2	39.706	136	70	4	35	169	2	126	3.83e-22	94.0

Find 35 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCTACCTCGCTCATCTTAA+TGG	0.206191	1_4:-4900689	None:intergenic
AGATGAGCGAGGTAGATTTC+TGG	0.267266	1_4:+4900695	Msa0145210:CDS
TTGTTTGATGTTGGATCATT+AGG	0.268362	1_4:-4900328	None:intergenic
TTTCACGGTGGATTTGTATT+TGG	0.297528	1_4:-4900251	None:intergenic
TTTAACCGACTATCACTAAT+AGG	0.305969	1_4:-4900577	None:intergenic
TGCTGCCCTGAGAATGTAAA+AGG	0.315281	1_4:-4900429	None:intergenic
TTTCAGATCTTCATGTTTGC+CGG	0.354972	1_4:+4900476	Msa0145210:CDS
TAATACCAGTGGCGAATTCC+CGG	0.370351	1_4:-4900495	None:intergenic
TAAATCAGGTTAAATTTCAA+TGG	0.370766	1_4:+4900596	Msa0145210:CDS
TAGTGATAGTCGGTTAAATC+AGG	0.383827	1_4:+4900582	Msa0145210:CDS
TTGATTACACCAGGAGTACT+AGG	0.399619	1_4:-4900277	None:intergenic
TGGTTTGTTTGACCCCTCTT+TGG	0.411608	1_4:-4900395	None:intergenic
TTAGGCTTGAAAACAAGTTT+CGG	0.417104	1_4:-4900310	None:intergenic
TCTACCTCGCTCATCTTAAT+GGG	0.430325	1_4:-4900688	None:intergenic
AGGAATGCCTGGTGAACTGC+TGG	0.469503	1_4:-4900655	None:intergenic
TGCTATTGCAGCAGCTGCTG+AGG	0.495654	1_4:+4900759	Msa0145210:CDS
GTTTGCCGGGAATTCGCCAC+TGG	0.495776	1_4:+4900490	Msa0145210:CDS
TTCAGATCTTCATGTTTGCC+GGG	0.499741	1_4:+4900477	Msa0145210:CDS
TGAAGCTTGTTGTTTGATGT+TGG	0.501548	1_4:-4900337	None:intergenic
CGAAAGTTAAAACAAAGGAT+TGG	0.516018	1_4:+4900523	Msa0145210:CDS
CGAGTCAATTTGATTACACC+AGG	0.517125	1_4:-4900286	None:intergenic
TCTTAATGGGTACAAAATTG+AGG	0.517891	1_4:-4900675	None:intergenic
TACGGTGATGTCTTCTTCGT+CGG	0.527447	1_4:+4900207	None:intergenic
GTGAAAGATCCTAGTACTCC+TGG	0.531540	1_4:+4900268	Msa0145210:CDS
AGGAGTACTAGGATCTTTCA+CGG	0.552743	1_4:-4900266	None:intergenic
ACTCAAACCAGCAGTTCACC+AGG	0.562161	1_4:+4900648	Msa0145210:CDS
TTAGCCGAAAGTTAAAACAA+AGG	0.594581	1_4:+4900518	Msa0145210:CDS
GTACAAAATTGAGGAATGCC+TGG	0.604789	1_4:-4900666	None:intergenic
CAAAGCCTATTAGTGATAGT+CGG	0.628800	1_4:+4900572	Msa0145210:CDS
CTTTCGGCTAATAATACCAG+TGG	0.640543	1_4:-4900506	None:intergenic
TGTACCCATTAAGATGAGCG+AGG	0.670287	1_4:+4900684	Msa0145210:CDS
GAATGTAAAAGGTTAAGCCA+CGG	0.676119	1_4:-4900418	None:intergenic
TGTAAAAGGTTAAGCCACGG+TGG	0.728400	1_4:-4900415	None:intergenic
AGTACTAGGATCTTTCACGG+TGG	0.769995	1_4:-4900263	None:intergenic
AGGGGTCAAACAAACCACCG+TGG	0.809962	1_4:+4900401	Msa0145210:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAATCAGGTTAAATTTCAA+TGG	+	chr1_4:4900596-4900615	Msa0145210:CDS	20.0%
!!!	AAAATCTGATATTTTATCTC+TGG	-	chr1_4:4900625-4900644	None:intergenic	20.0%
!!!	CAATCCTTTGTTTTAACTTT+CGG	-	chr1_4:4900525-4900544	None:intergenic	25.0%
	TTAGGCTTGAAAACAAGTTT+CGG	-	chr1_4:4900313-4900332	None:intergenic	30.0%
	TTGTTTGATGTTGGATCATT+AGG	-	chr1_4:4900331-4900350	None:intergenic	30.0%
	AAGTCAAAAAAACACCAAAG+AGG	+	chr1_4:4900381-4900400	Msa0145210:CDS	30.0%
	AGTCAAAAAAACACCAAAGA+GGG	+	chr1_4:4900382-4900401	Msa0145210:CDS	30.0%
!	CTTAACCTTTTACATTCTCA+GGG	+	chr1_4:4900424-4900443	Msa0145210:CDS	30.0%
	TTAGCCGAAAGTTAAAACAA+AGG	+	chr1_4:4900518-4900537	Msa0145210:CDS	30.0%
	CGAAAGTTAAAACAAAGGAT+TGG	+	chr1_4:4900523-4900542	Msa0145210:CDS	30.0%
	TTTAACCGACTATCACTAAT+AGG	-	chr1_4:4900580-4900599	None:intergenic	30.0%
!	TCTTAATGGGTACAAAATTG+AGG	-	chr1_4:4900678-4900697	None:intergenic	30.0%
!	TTTCACGGTGGATTTGTATT+TGG	-	chr1_4:4900254-4900273	None:intergenic	35.0%
!!	TGAAGCTTGTTGTTTGATGT+TGG	-	chr1_4:4900340-4900359	None:intergenic	35.0%
	GTCAAAAAAACACCAAAGAG+GGG	+	chr1_4:4900383-4900402	Msa0145210:CDS	35.0%
	GAATGTAAAAGGTTAAGCCA+CGG	-	chr1_4:4900421-4900440	None:intergenic	35.0%
!	GCTTAACCTTTTACATTCTC+AGG	+	chr1_4:4900423-4900442	Msa0145210:CDS	35.0%
	TTTCAGATCTTCATGTTTGC+CGG	+	chr1_4:4900476-4900495	Msa0145210:CDS	35.0%
	CAAAGCCTATTAGTGATAGT+CGG	+	chr1_4:4900572-4900591	Msa0145210:CDS	35.0%
	TAGTGATAGTCGGTTAAATC+AGG	+	chr1_4:4900582-4900601	Msa0145210:CDS	35.0%
!!	AGGAGTACTAGGATCTTTCA+CGG	-	chr1_4:4900269-4900288	None:intergenic	40.0%
!!	TTGATTACACCAGGAGTACT+AGG	-	chr1_4:4900280-4900299	None:intergenic	40.0%
	CGAGTCAATTTGATTACACC+AGG	-	chr1_4:4900289-4900308	None:intergenic	40.0%
	TTCAGATCTTCATGTTTGCC+GGG	+	chr1_4:4900477-4900496	Msa0145210:CDS	40.0%
	CTTTCGGCTAATAATACCAG+TGG	-	chr1_4:4900509-4900528	None:intergenic	40.0%
	GTACAAAATTGAGGAATGCC+TGG	-	chr1_4:4900669-4900688	None:intergenic	40.0%
	TCTACCTCGCTCATCTTAAT+GGG	-	chr1_4:4900691-4900710	None:intergenic	40.0%
	ATCTACCTCGCTCATCTTAA+TGG	-	chr1_4:4900692-4900711	None:intergenic	40.0%
!	AGCTGCTGCAATAGCATTTT+TGG	-	chr1_4:4900755-4900774	None:intergenic	40.0%
	AGTACTAGGATCTTTCACGG+TGG	-	chr1_4:4900266-4900285	None:intergenic	45.0%
	GTGAAAGATCCTAGTACTCC+TGG	+	chr1_4:4900268-4900287	Msa0145210:CDS	45.0%
!!	TGGTTTGTTTGACCCCTCTT+TGG	-	chr1_4:4900398-4900417	None:intergenic	45.0%
	TGTAAAAGGTTAAGCCACGG+TGG	-	chr1_4:4900418-4900437	None:intergenic	45.0%
	TGCTGCCCTGAGAATGTAAA+AGG	-	chr1_4:4900432-4900451	None:intergenic	45.0%
	TAATACCAGTGGCGAATTCC+CGG	-	chr1_4:4900498-4900517	None:intergenic	45.0%
	TGTACCCATTAAGATGAGCG+AGG	+	chr1_4:4900684-4900703	Msa0145210:CDS	45.0%
	AGATGAGCGAGGTAGATTTC+TGG	+	chr1_4:4900695-4900714	Msa0145210:CDS	45.0%
	ACTCAAACCAGCAGTTCACC+AGG	+	chr1_4:4900648-4900667	Msa0145210:CDS	50.0%
	AGGGGTCAAACAAACCACCG+TGG	+	chr1_4:4900401-4900420	Msa0145210:CDS	55.0%
	AGGAATGCCTGGTGAACTGC+TGG	-	chr1_4:4900658-4900677	None:intergenic	55.0%
	TGCTATTGCAGCAGCTGCTG+AGG	+	chr1_4:4900759-4900778	Msa0145210:CDS	55.0%
	GTTTGCCGGGAATTCGCCAC+TGG	+	chr1_4:4900490-4900509	Msa0145210:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	4900214	4900822	4900214	ID=Msa0145210;Name=Msa0145210
chr1_4	mRNA	4900214	4900822	4900214	ID=Msa0145210-mRNA-1;Parent=Msa0145210;Name=Msa0145210-mRNA-1;_AED=0.30;_eAED=0.32;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|202
chr1_4	exon	4900214	4900822	4900214	ID=Msa0145210-mRNA-1:exon:2060;Parent=Msa0145210-mRNA-1
chr1_4	CDS	4900214	4900822	4900214	ID=Msa0145210-mRNA-1:cds;Parent=Msa0145210-mRNA-1

Gene Sequence

>Msa0145210

ATGTCTTCTTCGTCGGTACAACAAGTTGTTGAGCGTGCCAAATACAAATCCACCGTGAAAGATCCTAGTACTCCTGGTGTAATCAAATTGACTCGACCGAAACTTGTTTTCAAGCCTAATGATCCAACATCAAACAACAAGCTTCACGTTGATTTCAGATTAATCAAAAGTCAAAAAAACACCAAAGAGGGGTCAAACAAACCACCGTGGCTTAACCTTTTACATTCTCAGGGCAGCAGTTACATCTTTGAGTTTGAAAGCTTTTCAGATCTTCATGTTTGCCGGGAATTCGCCACTGGTATTATTAGCCGAAAGTTAAAACAAAGGATTGGATTTAAAAATTCTTTGATCTTCGACACAAAGCCTATTAGTGATAGTCGGTTAAATCAGGTTAAATTTCAATGGACTCCAGAGATAAAATATCAGATTTTTGCACTCAAACCAGCAGTTCACCAGGCATTCCTCAATTTTGTACCCATTAAGATGAGCGAGGTAGATTTCTGGAACAAATACTTTAAAGCTGAATATCTCTATAGTACCAAAAATGCTATTGCAGCAGCTGCTGAGGCTGCTGAAGATGAAGATCTTGCTGTTTTTTTTGAAAGATGA

Protein sequence

>Msa0145210

MSSSSVQQVVERAKYKSTVKDPSTPGVIKLTRPKLVFKPNDPTSNNKLHVDFRLIKSQKNTKEGSNKPPWLNLLHSQGSSYIFEFESFSDLHVCREFATGIISRKLKQRIGFKNSLIFDTKPISDSRLNQVKFQWTPEIKYQIFALKPAVHQAFLNFVPIKMSEVDFWNKYFKAEYLYSTKNAIAAAAEAAEDEDLAVFFER*