AlfalfaGEDB | Msa0145550

M. sativa cultivar zhongmu-4 / Msa0145550

KEGG: -
GO: -
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0145550	XP_003589274.2	95.597	477	20	1	5	480	2	478	0.0	943

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0145550	sp\|Q8RYD1\|REM16_ARATH	33.784	296	182	7	172	457	11	302	8.03e-48	171
Msa0145550	sp\|Q8RYD1\|REM16_ARATH	32.323	297	147	8	14	261	11	302	3.70e-33	131

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0145550	G7I4K8	95.597	477	20	1	5	480	2	478	0.0	943

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TFs/TRs:

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Gene ID	Type	Classification
Msa0145550	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0145550	MtrunA17_Chr1g0154131	95.597	477	20	1	5	480	2	478	0.0	943
Msa0145550	MtrunA17_Chr1g0154071	57.803	519	144	8	1	474	1	489	0.0	561
Msa0145550	MtrunA17_Chr1g0154101	76.452	310	69	2	1	309	1	307	7.69e-171	502
Msa0145550	MtrunA17_Chr1g0154101	60.825	194	37	4	318	474	413	604	9.25e-65	224
Msa0145550	MtrunA17_Chr1g0154101	31.692	325	188	8	183	474	506	829	4.82e-37	145
Msa0145550	MtrunA17_Chr1g0154211	64.439	374	63	7	170	474	7	379	1.00e-160	461
Msa0145550	MtrunA17_Chr1g0154211	75.155	161	40	0	9	169	4	164	3.05e-81	257
Msa0145550	MtrunA17_Chr1g0154111	62.323	353	85	8	170	474	10	362	3.80e-143	423
Msa0145550	MtrunA17_Chr1g0154111	73.810	168	42	2	4	170	2	168	1.44e-78	256
Msa0145550	MtrunA17_Chr1g0154111	53.476	187	48	5	199	348	368	552	3.52e-46	169

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0145550	AT4G33280.1	33.784	296	182	7	172	457	11	302	8.16e-49	171
Msa0145550	AT4G33280.1	32.323	297	147	8	14	261	11	302	3.76e-34	131
Msa0145550	AT4G33280.2	46.951	164	86	1	172	334	11	174	2.20e-45	160
Msa0145550	AT4G33280.2	48.837	129	64	2	14	141	11	138	1.81e-34	131
Msa0145550	AT4G33280.3	46.951	164	86	1	172	334	11	174	6.26e-44	159
Msa0145550	AT4G33280.3	48.837	129	64	2	14	141	11	138	1.17e-33	131
Msa0145550	AT3G18990.2	24.069	349	195	9	183	475	5	339	7.04e-18	85.5
Msa0145550	AT3G18990.2	33.721	86	57	0	32	117	11	96	3.04e-11	65.5
Msa0145550	AT3G18990.1	24.269	342	189	9	183	468	5	332	8.55e-18	85.1
Msa0145550	AT3G18990.1	33.721	86	57	0	32	117	11	96	2.21e-11	65.5

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Find 104 sgRNAs with CRISPR-Local

Find 441 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAGGAGGTAAATGATT+CGG	0.471467	1_4:+5223303	None:intergenic
AAAATCGGTGCTAAGAAATT+GGG	0.464992	1_4:+5226523	None:intergenic
AAACAACAGCAGGTCTTGGG+AGG	0.625716	1_4:-5228227	Msa0145550:CDS
AAACTCCACTAGGACCTTTA+AGG	0.192051	1_4:+5226903	None:intergenic
AACAAAATAAGGAAAAGAGG+AGG	0.617290	1_4:+5223291	None:intergenic
AACAAGTTGCTGTTCAAAAT+CGG	0.433766	1_4:+5226508	None:intergenic
AACTCCACTAGGACCTTTAA+GGG	0.479312	1_4:+5225810	None:intergenic
AACTCCACTAGGACCTTTAA+GGG	0.479312	1_4:+5226904	None:intergenic
AAGGTCCTAGTGGAGTTGTG+TGG	0.624686	1_4:-5225804	Msa0145550:CDS
AAGTTGGAAAAGATGATTCT+AGG	0.330396	1_4:-5222100	Msa0145550:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTGAAGAAATTGGGTGAAA+CGG	+	chr1_4:5222168-5222187	None:intergenic	30.0%
TTTCTAAGCGGTTCATATTT+GGG	-	chr1_4:5222226-5222245	Msa0145550:CDS	30.0%
GCAATACAAACTCAAAACAA+AGG	+	chr1_4:5222435-5222454	None:intergenic	30.0%
GCAAAAGAATCATGCTAAAA+AGG	-	chr1_4:5222453-5222472	Msa0145550:intron	30.0%
GAAAGTGAAAAAAGAGATTG+TGG	-	chr1_4:5222485-5222504	Msa0145550:intron	30.0%
AAAAAAGAGATTGTGGTGTA+AGG	-	chr1_4:5222492-5222511	Msa0145550:intron	30.0%
AAAAAGAGATTGTGGTGTAA+GGG	-	chr1_4:5222493-5222512	Msa0145550:intron	30.0%
ATACAAGATAAAGAGAGAAG+AGG	+	chr1_4:5223037-5223056	None:intergenic	30.0%
AAACCATAAAGCATGTACTT+GGG	-	chr1_4:5223193-5223212	Msa0145550:intron	30.0%
CAATGTTTCATAGTCTCAAA+TGG	-	chr1_4:5223335-5223354	Msa0145550:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	5222088	5228272	5222088	ID=Msa0145550;Name=Msa0145550
chr1_4	mRNA	5222088	5228272	5222088	ID=Msa0145550-mRNA-1;Parent=Msa0145550;Name=Msa0145550-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0\|0\|0\|1\|1\|1\|5\|0\|480
chr1_4	exon	5228150	5228272	5228150	ID=Msa0145550-mRNA-1:exon:2183;Parent=Msa0145550-mRNA-1
chr1_4	exon	5226511	5226981	5226511	ID=Msa0145550-mRNA-1:exon:2182;Parent=Msa0145550-mRNA-1
chr1_4	exon	5225434	5225887	5225434	ID=Msa0145550-mRNA-1:exon:2181;Parent=Msa0145550-mRNA-1
chr1_4	exon	5223249	5223373	5223249	ID=Msa0145550-mRNA-1:exon:2180;Parent=Msa0145550-mRNA-1
chr1_4	exon	5222088	5222357	5222088	ID=Msa0145550-mRNA-1:exon:2179;Parent=Msa0145550-mRNA-1
chr1_4	CDS	5228150	5228272	5228150	ID=Msa0145550-mRNA-1:cds;Parent=Msa0145550-mRNA-1
chr1_4	CDS	5226511	5226981	5226511	ID=Msa0145550-mRNA-1:cds;Parent=Msa0145550-mRNA-1
chr1_4	CDS	5225434	5225887	5225434	ID=Msa0145550-mRNA-1:cds;Parent=Msa0145550-mRNA-1
chr1_4	CDS	5223249	5223373	5223249	ID=Msa0145550-mRNA-1:cds;Parent=Msa0145550-mRNA-1
chr1_4	CDS	5222088	5222357	5222088	ID=Msa0145550-mRNA-1:cds;Parent=Msa0145550-mRNA-1

Gene Sequence

>Msa0145550

ATGGGAGGAGGAGGAGGAACACCAAACAACAGCAGGTCTTGGGAGGAAGACATTTACTGGACCCATTTCCAATTCATCCGTTTCACCCAATTTCTTCAAACCACCCATTTGGAACAACAACTTGCACTTCCTAAAACATTTTCAGGCAATTTAAAGAACAAGTTGCCAGCAAATGTGACCCTTAAAGGTCCTAGTGGAGTTTTGTGGGATATAGGGCTGACAACTAGAGATGATACAGTTTACGCAACGAATGGTTGGCAACAATTCGTGAAAGATCACTCTTTGAAACAAAATGATTTCCTTGTCGTCAAGTACAATGGTGAATCACTCTTTGAGGTTTTAATCTTTCATGGAGAAAGCTTCTGTGAGAAAGCAGCTTCATATTTTATTCTTGAACGTGGACAAGCTCATACTGAACAAGGGGGTCGCAAGGCAAAGGACACAGACACTTCTGTTGAAGAAGTCAATACTCCTTCTAATGGCAGTGTTGAATGCGGTTTGCCTGAAAAATCATGGGAGGAAGACATTTACTGGACCCATTTCCAATTCATCCATTTCGCCCAATTTCTTAGCACCGATTTTGAACAGCAACTTGCACTTCCTAAAACATTCTCAGACAATTTAAAGAAGAAGTTGCCAGAAAATGTGACCCTTAAAGGTCCTAGTGGAGTTGTGTGGAATATAGGGTTGACAACTAGAGATGACACTGTTTACTTTGTGGACGGTTGGAAACGATTCATTAAAGATCACTCTTTGAAACAGAATGATTTCCTTGTCTTTAAGTACAACGGTAAATCACTCTTTGAGGTTTTAATCTTTGATGGAGATAGTTTCTGTGAGAAGGCAGCTTCTTATTTTGTTGGAAAATGTGGGAATGCTCCAACCGAACAAGGGGGTAGAAAGGCAAAAGACACAAATAAGTCTGTTTCCCCTGAGCAGTTCATGGCTGATGCGGTTCCCTCCCAAACAAACGGCAAAAGGACCAAGAAGAGGCCTGTTAATGAAGTTACGCCTGTCCAGACTAAGCGAGGAAGACGACCAAAAGCAGAGGCAGCACTGTCAAAGTTATCATCATCGCATGCTGAGAAAAAGATAGCCGAATCATTTACCTCCTCTTTTCCTTATTTTGTTAAAATGATCAAAACGTTCAATGTCGATGGTCCACGTATTCTGAATGTACCCCACCAATTCTCAAAGGCACATCTTCCAAACCGCAAGATAAAGATTATTCTTCATAATTTGAAGGGAGAACAATGGACTGTTAATTCTGTTCCAACGACTAGAGTGAATACAGATCACACTCTATGTGGAGGATGGGTGAATTTTGTTCGTGGAAACAACATAAAAGTTGGAGATGTCTGCATTTTCGAACTTATACATGAGTGTGAATTACGTGTTCGCGTCGCTGAAGTTGGAAAAGATGATTCTAGGTTGGAAAACTAG

Protein sequence

>Msa0145550

MGGGGGTPNNSRSWEEDIYWTHFQFIRFTQFLQTTHLEQQLALPKTFSGNLKNKLPANVTLKGPSGVLWDIGLTTRDDTVYATNGWQQFVKDHSLKQNDFLVVKYNGESLFEVLIFHGESFCEKAASYFILERGQAHTEQGGRKAKDTDTSVEEVNTPSNGSVECGLPEKSWEEDIYWTHFQFIHFAQFLSTDFEQQLALPKTFSDNLKKKLPENVTLKGPSGVVWNIGLTTRDDTVYFVDGWKRFIKDHSLKQNDFLVFKYNGKSLFEVLIFDGDSFCEKAASYFVGKCGNAPTEQGGRKAKDTNKSVSPEQFMADAVPSQTNGKRTKKRPVNEVTPVQTKRGRRPKAEAALSKLSSSHAEKKIAESFTSSFPYFVKMIKTFNVDGPRILNVPHQFSKAHLPNRKIKIILHNLKGEQWTVNSVPTTRVNTDHTLCGGWVNFVRGNNIKVGDVCIFELIHECELRVRVAEVGKDDSRLEN*