AlfalfaGEDB | Msa0148220

M. sativa cultivar zhongmu-4 / Msa0148220

KEGG: -
GO: GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003714 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006950 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009889 GO:0009890 GO:0009892 GO:0010035 GO:0010556 GO:0010558 GO:0010605 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901700 GO:1902679 GO:1903506 GO:1903507 GO:2001141
PFAM: PF00628.32

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0148220	sp\|Q5XEM9\|ALFL5_ARATH	88.136	59	7	0	23	81	202	260	6.19e-35	122

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0148220	A0A1S3E6R3	84.146	82	9	2	3	81	228	308	7.98e-38	137

TFs/TRs:

Gene ID	Type	Classification
Msa0148220	TF	Alfin-like

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0148220	MtrunA17_Chr1g0150821	78.824	85	14	1	1	81	179	263	5.36e-37	124
Msa0148220	MtrunA17_Chr8g0343441	88.333	60	6	1	23	81	197	256	2.93e-34	117
Msa0148220	MtrunA17_Chr2g0329821	87.931	58	6	1	24	80	193	250	2.18e-31	109
Msa0148220	MtrunA17_Chr2g0300241	83.929	56	7	1	28	81	198	253	9.56e-30	105
Msa0148220	MtrunA17_Chr4g0006381	67.089	79	25	1	4	81	164	242	6.33e-29	103
Msa0148220	MtrunA17_Chr4g0076771	81.132	53	10	0	28	80	202	254	2.58e-28	102
Msa0148220	MtrunA17_Chr7g0242511	75.000	56	14	0	26	81	187	242	5.03e-27	98.2

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0148220	AT5G20510.2	88.136	59	7	23	81	161	219	3.18e-36	121
Msa0148220	AT5G20510.1	88.136	59	7	23	81	202	260	6.29e-36	122
Msa0148220	AT3G42790.1	91.379	58	5	23	80	192	249	2.35e-35	120
Msa0148220	AT5G26210.1	89.091	55	6	26	80	200	254	1.35e-31	110
Msa0148220	AT2G02470.1	83.636	55	9	27	81	202	256	7.28e-31	108
Msa0148220	AT2G02470.2	83.636	55	9	27	81	193	247	8.00e-31	108
Msa0148220	AT5G05610.2	59.740	77	31	4	80	164	240	2.13e-28	102
Msa0148220	AT5G05610.1	59.740	77	31	4	80	164	240	2.13e-28	102
Msa0148220	AT3G11200.1	68.966	58	18	23	80	188	245	2.13e-26	97.1
Msa0148220	AT3G11200.2	68.966	58	18	23	80	175	232	2.37e-26	96.7
Msa0148220	AT1G14510.1	75.000	52	13	27	78	197	248	8.70e-26	95.9

Find 21 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTATGCAGCTGATGAGTTC+TGG	0.348702	1_4:+8463073	Msa0148220:CDS
TTGATATGCTCAGCCCTTGC+CGG	0.382099	1_4:-8463150	None:intergenic
GCAGTGACATTTGCGAGAAA+TGG	0.391588	1_4:+8463100	Msa0148220:CDS
ATTTGCGAGAAATGGTTTCA+TGG	0.414893	1_4:+8463108	Msa0148220:CDS
GTCAAAGGACGAGGAGGTTG+AGG	0.459920	1_4:+8462990	Msa0148220:CDS
GCTCTCTTGTTGCTGCAAGC+TGG	0.467608	1_4:-8463186	None:intergenic
CGTGAAGATTACTCCGGCAA+GGG	0.576974	1_4:+8463137	Msa0148220:CDS
GGTTGAGGATGGTGTAGGTG+TGG	0.578977	1_4:+8463005	Msa0148220:CDS
GAGGAGGTTGAGGATGGTGT+AGG	0.579309	1_4:+8463000	Msa0148220:CDS
GCGTGAAGATTACTCCGGCA+AGG	0.589663	1_4:+8463136	Msa0148220:CDS
GGCAATGCAAATGAAGTCAA+AGG	0.599376	1_4:+8462975	Msa0148220:CDS
GCAAATGAAGTCAAAGGACG+AGG	0.610740	1_4:+8462981	Msa0148220:CDS
GAGACTTTGTGTGGGGCATG+TGG	0.613065	1_4:+8463045	Msa0148220:CDS
AAGGACGAGGAGGTTGAGGA+TGG	0.620188	1_4:+8462994	Msa0148220:CDS
AACACGGAGAGACTTTGTGT+GGG	0.622939	1_4:+8463037	Msa0148220:CDS
GGATGGTGTAGGTGTGGACG+AGG	0.628142	1_4:+8463011	Msa0148220:CDS
GAACACGGAGAGACTTTGTG+TGG	0.630911	1_4:+8463036	Msa0148220:CDS
ACACGGAGAGACTTTGTGTG+GGG	0.707279	1_4:+8463038	Msa0148220:CDS
AATGAAGTCAAAGGACGAGG+AGG	0.714948	1_4:+8462984	Msa0148220:CDS
AAAATGCGTGAAGATTACTC+CGG	0.724223	1_4:+8463131	Msa0148220:CDS
GGTGTGGACGAGGAAGAACA+CGG	0.729144	1_4:+8463021	Msa0148220:CDS

CRISPR-GE

sgRNA_sequence	Strand	Position	Region	GC_content
ATTTGCGAGAAATGGTTTCA+TGG	+	chr1_4:8463108-8463127	Msa0148220:CDS	35.0%
AAAATGCGTGAAGATTACTC+CGG	+	chr1_4:8463131-8463150	Msa0148220:CDS	35.0%
GGCAATGCAAATGAAGTCAA+AGG	+	chr1_4:8462975-8462994	Msa0148220:CDS	40.0%
GCAAATGAAGTCAAAGGACG+AGG	+	chr1_4:8462981-8463000	Msa0148220:CDS	45.0%
AATGAAGTCAAAGGACGAGG+AGG	+	chr1_4:8462984-8463003	Msa0148220:CDS	45.0%
AACACGGAGAGACTTTGTGT+GGG	+	chr1_4:8463037-8463056	Msa0148220:CDS	45.0%
GCAGTGACATTTGCGAGAAA+TGG	+	chr1_4:8463100-8463119	Msa0148220:CDS	45.0%
GAACACGGAGAGACTTTGTG+TGG	+	chr1_4:8463036-8463055	Msa0148220:CDS	50.0%
ACACGGAGAGACTTTGTGTG+GGG	+	chr1_4:8463038-8463057	Msa0148220:CDS	50.0%
GCTATGCAGCTGATGAGTTC+TGG	+	chr1_4:8463073-8463092	Msa0148220:CDS	50.0%
CGTGAAGATTACTCCGGCAA+GGG	+	chr1_4:8463137-8463156	Msa0148220:CDS	50.0%
TTGATATGCTCAGCCCTTGC+CGG	-	chr1_4:8463153-8463172	None:intergenic	50.0%
GTCAAAGGACGAGGAGGTTG+AGG	+	chr1_4:8462990-8463009	Msa0148220:CDS	55.0%
AAGGACGAGGAGGTTGAGGA+TGG	+	chr1_4:8462994-8463013	Msa0148220:CDS	55.0%
GAGGAGGTTGAGGATGGTGT+AGG	+	chr1_4:8463000-8463019	Msa0148220:CDS	55.0%
GGTTGAGGATGGTGTAGGTG+TGG	+	chr1_4:8463005-8463024	Msa0148220:CDS	55.0%
GGTGTGGACGAGGAAGAACA+CGG	+	chr1_4:8463021-8463040	Msa0148220:CDS	55.0%
GAGACTTTGTGTGGGGCATG+TGG	+	chr1_4:8463045-8463064	Msa0148220:CDS	55.0%
GCGTGAAGATTACTCCGGCA+AGG	+	chr1_4:8463136-8463155	Msa0148220:CDS	55.0%
GCTCTCTTGTTGCTGCAAGC+TGG	-	chr1_4:8463189-8463208	None:intergenic	55.0%
GGATGGTGTAGGTGTGGACG+AGG	+	chr1_4:8463011-8463030	Msa0148220:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	8462973	8463218	8462973	ID=Msa0148220;Name=Msa0148220
chr1_4	mRNA	8462973	8463218	8462973	ID=Msa0148220-mRNA-1;Parent=Msa0148220;Name=Msa0148220-mRNA-1;_AED=0.45;_eAED=0.45;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|81
chr1_4	exon	8462973	8463218	8462973	ID=Msa0148220-mRNA-1:exon:3633;Parent=Msa0148220-mRNA-1
chr1_4	CDS	8462973	8463218	8462973	ID=Msa0148220-mRNA-1:cds;Parent=Msa0148220-mRNA-1

Gene Sequence	>Msa0148220 ATGGCAATGCAAATGAAGTCAAAGGACGAGGAGGTTGAGGATGGTGTAGGTGTGGACGAGGAAGAACACGGAGAGACTTTGTGTGGGGCATGTGGAGACAGCTATGCAGCTGATGAGTTCTGGATTTGCAGTGACATTTGCGAGAAATGGTTTCATGGAAAATGCGTGAAGATTACTCCGGCAAGGGCTGAGCATATCAAACAGTACAAATGTCCAGCTTGCAGCAACAAGAGAGCTCGCCCTTGA
Protein sequence	>Msa0148220 MAMQMKSKDEEVEDGVGVDEEEHGETLCGACGDSYAADEFWICSDICEKWFHGKCVKITPARAEHIKQYKCPACSNKRARP*