Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0148950 | KEH39848.1 | 65.714 | 70 | 23 | 1 | 9 | 78 | 603 | 671 | 8.15e-19 | 90.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0148950 | A0A072VEL2 | 65.714 | 70 | 23 | 1 | 9 | 78 | 603 | 671 | 3.89e-19 | 90.1 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0148950 | TR | SET |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0148950 | MtrunA17_Chr1g0149611 | 65.714 | 70 | 23 | 1 | 9 | 78 | 440 | 508 | 1.02e-22 | 89.7 |
| Msa0148950 | MtrunA17_Chr1g0149581 | 54.286 | 70 | 31 | 1 | 9 | 78 | 1598 | 1666 | 2.88e-17 | 74.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0148950 | AT3G61740.1 | 48.889 | 45 | 23 | 0 | 34 | 78 | 974 | 1018 | 9.39e-11 | 56.2 |
| Msa0148950 | AT3G61740.2 | 48.889 | 45 | 23 | 0 | 34 | 78 | 938 | 982 | 9.66e-11 | 56.2 |
Find 23 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGCAATTTATTGAATTGC+AGG | 0.310671 | 1_4:-9406647 | None:intergenic |
| CTACACCGATTCCAATATTC+CGG | 0.336164 | 1_4:+9406208 | Msa0148950:CDS |
| AAGGAGGAATCCCGGAATAT+TGG | 0.345198 | 1_4:-9406219 | None:intergenic |
| TGCTATTTCAGATGCATTGA+CGG | 0.383868 | 1_4:+9406269 | Msa0148950:CDS |
| CCTGCAATTCAATAAATTGC+AGG | 0.392271 | 1_4:+9406647 | Msa0148950:CDS |
| GGAATCGGTGTAGTCAAATT+CGG | 0.412765 | 1_4:-9406198 | None:intergenic |
| ATGAGTGGCGGTGGGGATGA+AGG | 0.438746 | 1_4:-9406238 | None:intergenic |
| CTTCTTCTTCACATTTAAAG+TGG | 0.459198 | 1_4:-9406603 | None:intergenic |
| TTTATTGAATTGCAGGAACA+TGG | 0.509510 | 1_4:-9406640 | None:intergenic |
| GCAAAGAGGGACATATTAGC+TGG | 0.519874 | 1_4:+9406559 | Msa0148950:CDS |
| GGTTATCTTCTTTGCAAAGA+GGG | 0.527796 | 1_4:+9406546 | Msa0148950:intron |
| TGGGGATGAAGGAGGAATCC+CGG | 0.531853 | 1_4:-9406227 | None:intergenic |
| AGTGGCGGTGGGGATGAAGG+AGG | 0.545347 | 1_4:-9406235 | None:intergenic |
| GAATCCCGGAATATTGGAAT+CGG | 0.547638 | 1_4:-9406213 | None:intergenic |
| TACACCGATTCCAATATTCC+GGG | 0.550942 | 1_4:+9406209 | Msa0148950:CDS |
| AGGTGAATGATGAGTGGCGG+TGG | 0.556064 | 1_4:-9406247 | None:intergenic |
| GTCAATGCATCTGAAATAGC+AGG | 0.561079 | 1_4:-9406267 | None:intergenic |
| GGTGAATGATGAGTGGCGGT+GGG | 0.582510 | 1_4:-9406246 | None:intergenic |
| TTATTGAATTGCAGGAACAT+GGG | 0.589124 | 1_4:-9406639 | None:intergenic |
| AGGTTATCTTCTTTGCAAAG+AGG | 0.615001 | 1_4:+9406545 | Msa0148950:intron |
| GTGAATGATGAGTGGCGGTG+GGG | 0.682408 | 1_4:-9406245 | None:intergenic |
| AGCAGGTGAATGATGAGTGG+CGG | 0.714715 | 1_4:-9406250 | None:intergenic |
| AATAGCAGGTGAATGATGAG+TGG | 0.746575 | 1_4:-9406253 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAACATTTTCAATGATACAA+TGG | + | chr1_4:9406343-9406362 | Msa0148950:intron | 20.0% |
| !! | AGGTCATGTCTAATATTTTA+TGG | - | chr1_4:9406425-9406444 | None:intergenic | 25.0% |
| !!! | AATTGATCAAGTTTTATCTC+AGG | - | chr1_4:9406445-9406464 | None:intergenic | 25.0% |
| GGAATTGAATAGAAGATGAA+AGG | - | chr1_4:9406490-9406509 | None:intergenic | 30.0% | |
| !! | CACTTAGTTTTCTCATATGT+CGG | - | chr1_4:9406511-9406530 | None:intergenic | 30.0% |
| AAGAAGATAACCTACAAAAC+CGG | - | chr1_4:9406538-9406557 | None:intergenic | 30.0% | |
| CTTCTTCTTCACATTTAAAG+TGG | - | chr1_4:9406606-9406625 | None:intergenic | 30.0% | |
| TTATTGAATTGCAGGAACAT+GGG | - | chr1_4:9406642-9406661 | None:intergenic | 30.0% | |
| TTTATTGAATTGCAGGAACA+TGG | - | chr1_4:9406643-9406662 | None:intergenic | 30.0% | |
| !! | TGCTATTTCAGATGCATTGA+CGG | + | chr1_4:9406269-9406288 | Msa0148950:CDS | 35.0% |
| TGAGAAAACTAAGTGTCAAC+CGG | + | chr1_4:9406516-9406535 | Msa0148950:intron | 35.0% | |
| AGGTTATCTTCTTTGCAAAG+AGG | + | chr1_4:9406545-9406564 | Msa0148950:intron | 35.0% | |
| GGTTATCTTCTTTGCAAAGA+GGG | + | chr1_4:9406546-9406565 | Msa0148950:intron | 35.0% | |
| CCTGCAATTTATTGAATTGC+AGG | - | chr1_4:9406650-9406669 | None:intergenic | 35.0% | |
| CCTGCAATTCAATAAATTGC+AGG | + | chr1_4:9406647-9406666 | Msa0148950:CDS | 35.0% | |
| GGAATCGGTGTAGTCAAATT+CGG | - | chr1_4:9406201-9406220 | None:intergenic | 40.0% | |
| !! | CTACACCGATTCCAATATTC+CGG | + | chr1_4:9406208-9406227 | Msa0148950:CDS | 40.0% |
| !! | TACACCGATTCCAATATTCC+GGG | + | chr1_4:9406209-9406228 | Msa0148950:CDS | 40.0% |
| GAATCCCGGAATATTGGAAT+CGG | - | chr1_4:9406216-9406235 | None:intergenic | 40.0% | |
| AATAGCAGGTGAATGATGAG+TGG | - | chr1_4:9406256-9406275 | None:intergenic | 40.0% | |
| ! | GTCAATGCATCTGAAATAGC+AGG | - | chr1_4:9406270-9406289 | None:intergenic | 40.0% |
| AAAGGTGAATGCAGAAATGC+AGG | - | chr1_4:9406472-9406491 | None:intergenic | 40.0% | |
| !! | TAAGTGTCAACCGGTTTTGT+AGG | + | chr1_4:9406525-9406544 | Msa0148950:intron | 40.0% |
| AAGGAGGAATCCCGGAATAT+TGG | - | chr1_4:9406222-9406241 | None:intergenic | 45.0% | |
| GCAAAGAGGGACATATTAGC+TGG | + | chr1_4:9406559-9406578 | Msa0148950:CDS | 45.0% | |
| AGCAGGTGAATGATGAGTGG+CGG | - | chr1_4:9406253-9406272 | None:intergenic | 50.0% | |
| TGGGGATGAAGGAGGAATCC+CGG | - | chr1_4:9406230-9406249 | None:intergenic | 55.0% | |
| GTGAATGATGAGTGGCGGTG+GGG | - | chr1_4:9406248-9406267 | None:intergenic | 55.0% | |
| GGTGAATGATGAGTGGCGGT+GGG | - | chr1_4:9406249-9406268 | None:intergenic | 55.0% | |
| AGGTGAATGATGAGTGGCGG+TGG | - | chr1_4:9406250-9406269 | None:intergenic | 55.0% | |
| ATGAGTGGCGGTGGGGATGA+AGG | - | chr1_4:9406241-9406260 | None:intergenic | 60.0% | |
| AGTGGCGGTGGGGATGAAGG+AGG | - | chr1_4:9406238-9406257 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_4 | gene | 9406192 | 9406684 | 9406192 | ID=Msa0148950;Name=Msa0148950 |
| chr1_4 | mRNA | 9406192 | 9406684 | 9406192 | ID=Msa0148950-mRNA-1;Parent=Msa0148950;Name=Msa0148950-mRNA-1;_AED=0.71;_eAED=0.71;_QI=0|0|0|0.5|0|0|2|0|78 |
| chr1_4 | exon | 9406192 | 9406290 | 9406192 | ID=Msa0148950-mRNA-1:exon:4013;Parent=Msa0148950-mRNA-1 |
| chr1_4 | exon | 9406547 | 9406684 | 9406547 | ID=Msa0148950-mRNA-1:exon:4014;Parent=Msa0148950-mRNA-1 |
| chr1_4 | CDS | 9406192 | 9406290 | 9406192 | ID=Msa0148950-mRNA-1:cds;Parent=Msa0148950-mRNA-1 |
| chr1_4 | CDS | 9406547 | 9406684 | 9406547 | ID=Msa0148950-mRNA-1:cds;Parent=Msa0148950-mRNA-1 |
| Gene Sequence |
| Protein sequence |
