Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0150600 | RHN77780.1 | 85.106 | 423 | 61 | 2 | 1 | 422 | 311 | 732 | 0.0 | 734 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0150600 | sp|P93604|LRK10_WHEAT | 54.734 | 338 | 148 | 3 | 46 | 378 | 289 | 626 | 2.12e-125 | 379 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0150600 | A0A396JHQ4 | 85.106 | 423 | 61 | 2 | 1 | 422 | 311 | 732 | 0.0 | 734 |
Co-expression Network
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0150600 | MtrunA17_Chr1g0158411 | 85.106 | 423 | 61 | 2 | 1 | 422 | 311 | 732 | 0.0 | 734 |
| Msa0150600 | MtrunA17_Chr6g0467711 | 81.592 | 402 | 72 | 2 | 10 | 410 | 315 | 715 | 0.0 | 676 |
| Msa0150600 | MtrunA17_Chr3g0088321 | 85.526 | 380 | 51 | 1 | 43 | 422 | 1 | 376 | 0.0 | 668 |
| Msa0150600 | MtrunA17_Chr1g0158341 | 84.831 | 356 | 50 | 1 | 67 | 422 | 1 | 352 | 0.0 | 624 |
| Msa0150600 | MtrunA17_Chr1g0158441 | 85.154 | 357 | 51 | 2 | 67 | 422 | 1 | 356 | 0.0 | 622 |
| Msa0150600 | MtrunA17_Chr1g0158751 | 68.735 | 419 | 118 | 4 | 15 | 422 | 6 | 422 | 0.0 | 586 |
| Msa0150600 | MtrunA17_Chr1g0157931 | 71.990 | 407 | 108 | 5 | 17 | 418 | 192 | 597 | 0.0 | 582 |
| Msa0150600 | MtrunA17_Chr1g0157911 | 71.744 | 407 | 109 | 5 | 17 | 418 | 318 | 723 | 0.0 | 580 |
| Msa0150600 | MtrunA17_Chr1g0157881 | 71.990 | 407 | 108 | 5 | 17 | 418 | 385 | 790 | 0.0 | 568 |
| Msa0150600 | MtrunA17_Chr1g0158571 | 70.588 | 391 | 110 | 3 | 30 | 418 | 147 | 534 | 0.0 | 566 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0150600 | AT5G38280.1 | 50.313 | 320 | 152 | 5 | 78 | 391 | 314 | 632 | 5.03e-110 | 338 |
| Msa0150600 | AT5G38280.2 | 50.313 | 320 | 152 | 5 | 78 | 391 | 314 | 632 | 7.80e-110 | 338 |
| Msa0150600 | AT5G38240.3 | 47.977 | 346 | 162 | 5 | 42 | 381 | 221 | 554 | 2.26e-108 | 331 |
| Msa0150600 | AT5G38250.1 | 45.128 | 390 | 182 | 9 | 21 | 405 | 252 | 614 | 2.53e-108 | 332 |
| Msa0150600 | AT5G38240.2 | 45.777 | 367 | 175 | 6 | 42 | 402 | 267 | 615 | 1.11e-107 | 331 |
| Msa0150600 | AT5G38240.1 | 46.322 | 367 | 173 | 7 | 42 | 402 | 285 | 633 | 1.40e-107 | 331 |
| Msa0150600 | AT4G18250.1 | 45.038 | 393 | 193 | 10 | 35 | 413 | 295 | 678 | 2.29e-107 | 332 |
| Msa0150600 | AT1G70250.1 | 52.564 | 312 | 135 | 7 | 78 | 380 | 442 | 749 | 8.13e-107 | 333 |
| Msa0150600 | AT5G38260.1 | 50.154 | 325 | 157 | 3 | 83 | 403 | 309 | 632 | 2.49e-106 | 328 |
| Msa0150600 | AT5G39020.1 | 46.512 | 344 | 176 | 4 | 40 | 379 | 442 | 781 | 2.68e-106 | 332 |
Find 80 sgRNAs with CRISPR-Local
Find 180 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGTTCCGAATTAACCCG+CGG | 0.756037 | 1_4:-12215432 | Msa0150600:CDS |
| AAAATGTATGAGATATCCCT+TGG | 0.511286 | 1_4:-12214871 | Msa0150600:CDS |
| AAACTTGGTGAAGGAGGTTT+TGG | 0.303015 | 1_4:-12215147 | Msa0150600:CDS |
| AAAGATGACTCGAAGTTTCA+AGG | 0.492767 | 1_4:-12215172 | Msa0150600:CDS |
| AAAGATGTTGGGTAAATCTA+AGG | 0.259376 | 1_4:-12215079 | Msa0150600:CDS |
| AAAGGTTCCGCGGGTTAATT+CGG | 0.349127 | 1_4:+12215425 | None:intergenic |
| AAAGTCATAGAGATGCTTGA+AGG | 0.495366 | 1_4:-12214367 | Msa0150600:CDS |
| AACTTTCTTAGATAATCCAA+AGG | 0.597782 | 1_4:+12215407 | None:intergenic |
| AAGACCAAAGTCTGAAACCT+TGG | 0.449938 | 1_4:+12214747 | None:intergenic |
| AAGAGGAACAATTGGTTACA+TGG | 0.550416 | 1_4:-12214677 | Msa0150600:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TATAAAGGAAAGTTAAGCAG+TGG | - | chr1_4:12214525-12214544 | Msa0150600:CDS | 30.0% | |
| ATAAAGGAAAGTTAAGCAGT+GGG | - | chr1_4:12214526-12214545 | Msa0150600:CDS | 30.0% | |
| AGATTTACCCAACATCTTTA+TGG | + | chr1_4:12214562-12214581 | None:intergenic | 30.0% | |
| AAAGATGTTGGGTAAATCTA+AGG | - | chr1_4:12214563-12214582 | Msa0150600:CDS | 30.0% | |
| AAGATGTTGGGTAAATCTAA+GGG | - | chr1_4:12214564-12214583 | Msa0150600:CDS | 30.0% | |
| ATAAATGAAGTTGCAACCAT+AGG | - | chr1_4:12214603-12214622 | Msa0150600:CDS | 30.0% | |
| TATTTATCAAGAGAGCCATT+GGG | + | chr1_4:12214711-12214730 | None:intergenic | 30.0% | |
| ATATTTATCAAGAGAGCCAT+TGG | + | chr1_4:12214712-12214731 | None:intergenic | 30.0% | |
| AAAATGTATGAGATATCCCT+TGG | - | chr1_4:12214771-12214790 | Msa0150600:CDS | 30.0% | |
| TGATCAATTGCTTGAAGAAA+AGG | - | chr1_4:12215142-12215161 | Msa0150600:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_4 | gene | 12214198 | 12215466 | 12214198 | ID=Msa0150600;Name=Msa0150600 |
| chr1_4 | mRNA | 12214198 | 12215466 | 12214198 | ID=Msa0150600-mRNA-1;Parent=Msa0150600;Name=Msa0150600-mRNA-1;_AED=0.80;_eAED=0.80;_QI=0|-1|0|1|-1|1|1|0|422 |
| chr1_4 | exon | 12214198 | 12215466 | 12214198 | ID=Msa0150600-mRNA-1:exon:4990;Parent=Msa0150600-mRNA-1 |
| chr1_4 | CDS | 12214198 | 12215466 | 12214198 | ID=Msa0150600-mRNA-1:cds;Parent=Msa0150600-mRNA-1 |
| Gene Sequence |
>Msa0150600 ATGGGTGCAACAAAAAGTTCCGAATTAACCCGCGGAACCTTTGGATTATCTAAGAAAGTTTCTGATTCTAAATTTAGCATCGAGTTTGGAAGAATCATTGGCAGGTATTTCTTGCCAATATTTATAGTGAGATTTACACTGGGCCTCATGGTTTTTTTTGTGTTATTAACATATACTTGTCGAAGAAGACATATATCAATATACGAAAATATTGAAGTTTTTCTTAGAGGGAGTACCTTGATGCCAAGAAGGTATTCGTACAAAGATTTGAAAAAGATGACTCGAAGTTTCAAGGACAAACTTGGTGAAGGAGGTTTTGGTTCTGTTTATAAAGGAAAGTTAAGCAGTGGGTCATCTGTGGCCATAAAGATGTTGGGTAAATCTAAGGGTAATGGACAAGATTTTATAAATGAAGTTGCAACCATAGGACGAATACACCATGAAAATGTGGTGAGACTTATTGGTTTTTGTTTCGAGAGATCGAAACATGCTCTAGTTTATGAATTCATGCCCAATGGCTCTCTTGATAAATATATTTCTACTAAGGCGGATGCTATCTCTCTAACTTCAAAAAAAATGTATGAGATATCCCTTGGAGTGGCTCGCGGAATTGCTTATTTGCACCAAGGTTGTGACATGCAAATCTTGCATTTTGATATCAAACCCCATAACATCCTTCTTGACGAGAATTTTATCCCCAAGGTTTCAGACTTTGGTCTTGCAAAACTATATCCAAATGACAATAGTATTGTTACTTTAACTGCAGCAAGAGGAACAATTGGTTACATGGCTCCTGAATTGTTCTACAAAAATATTGGAGGAGTATCCTACAAGGCTGATGTATATAGCTTTGGAATGCTTTTGATGGAAATGGCAAGTAAGAAAAGAAATTTAAATCGACATGCCGATCGTTCAAGTCAAACTTTCTTTCCCATTTGGATTTATGATCAATTGCTTGAAGAAAAGGAGAGAGAAATGGATGAAATTACTTATGAGGAAAATAATAATGTGAAGAAGATGTTGATAATTGCATTATGGTGTATACAATTGAAACCATGTGATCGTCCTTCAATGAACAAAGTCATAGAGATGCTTGAAGGAGATATTGAAAATATTGAAATGCCTCCAAAGCCCTCTTTGTATCCAAATGAAATGATTCATGAAGATCTTGAAGTCAACTCTAATGAAAATGACTCAGAAACTGATGTTGGCTCTCTTAGTTTTATTGATGAAGATACAACAGAACCTCTATTAATGAATTCTTCTTGA |
| Protein sequence |
>Msa0150600 MGATKSSELTRGTFGLSKKVSDSKFSIEFGRIIGRYFLPIFIVRFTLGLMVFFVLLTYTCRRRHISIYENIEVFLRGSTLMPRRYSYKDLKKMTRSFKDKLGEGGFGSVYKGKLSSGSSVAIKMLGKSKGNGQDFINEVATIGRIHHENVVRLIGFCFERSKHALVYEFMPNGSLDKYISTKADAISLTSKKMYEISLGVARGIAYLHQGCDMQILHFDIKPHNILLDENFIPKVSDFGLAKLYPNDNSIVTLTAARGTIGYMAPELFYKNIGGVSYKADVYSFGMLLMEMASKKRNLNRHADRSSQTFFPIWIYDQLLEEKEREMDEITYEENNNVKKMLIIALWCIQLKPCDRPSMNKVIEMLEGDIENIEMPPKPSLYPNEMIHEDLEVNSNENDSETDVGSLSFIDEDTTEPLLMNSS* |
