AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0153040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0153040 MtrunA17_Chr1g0162411 74.653 288 48 3 1 266 107 391 5.57e-139 400
Msa0153040 MtrunA17_Chr1g0162441 53.754 333 86 9 1 306 109 400 1.70e-90 276
Msa0153040 MtrunA17_Chr4g0033961 41.956 317 117 10 16 307 123 397 2.26e-63 206
Msa0153040 MtrunA17_Chr1g0162431 69.811 53 16 0 251 303 5 57 2.00e-17 75.5
Msa0153040 MtrunA17_Chr1g0208771 34.746 118 68 4 191 306 188 298 1.92e-14 73.6
Msa0153040 MtrunA17_Chr8g0390841 28.638 213 126 9 111 303 130 336 8.71e-13 68.6
Msa0153040 MtrunA17_Chr3g0103201 32.203 118 59 4 190 297 114 220 1.32e-12 66.2
Msa0153040 MtrunA17_Chr3g0103161 32.143 112 65 5 190 296 17 122 2.67e-12 63.2
Msa0153040 MtrunA17_Chr3g0131891 31.967 122 77 2 183 298 182 303 4.97e-12 65.9
Msa0153040 MtrunA17_Chr1g0163071 33.913 115 67 4 193 305 187 294 1.03e-11 64.7
Msa0153040 MtrunA17_Chr3g0077421 35.537 121 65 4 185 299 84 197 2.23e-11 62.4
Msa0153040 MtrunA17_Chr3g0077441 35.537 121 65 4 185 299 84 197 2.23e-11 62.4
Msa0153040 MtrunA17_Chr3g0077361 35.246 122 64 6 183 298 389 501 5.91e-11 63.2
Msa0153040 MtrunA17_Chr5g0429931 34.507 142 81 6 160 298 110 242 6.10e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0153040 AT1G49480.3 53.552 183 52 4 133 301 61 224 2.57e-55 179
Msa0153040 AT1G49480.1 53.552 183 52 4 133 301 61 224 2.57e-55 179
Msa0153040 AT1G49480.2 53.552 183 52 4 133 301 61 224 2.57e-55 179
Msa0153040 AT3G18990.1 49.254 201 69 4 115 301 158 339 1.29e-52 176
Msa0153040 AT3G18990.2 48.756 201 70 4 115 301 158 339 2.16e-51 174

Find 80 sgRNAs with CRISPR-Local

Find 181 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAATTTGAAGGGTGTTTC+TGG 0.161265 1_4:-16125609 Msa0153040:CDS
GTTATATAATATAACATGAT+AGG 0.259067 1_4:+16125020 None:intergenic
TATCGACTCCTTTGATTTCC+TGG 0.264885 1_4:-16127935 Msa0153040:CDS
TTTACACAAGAGAACGGTTT+AGG 0.281466 1_4:-16125489 Msa0153040:CDS
ATTCTCTAGTTTATTCAGTT+TGG 0.286188 1_4:-16125268 Msa0153040:three_prime_UTR
CAGTATTTGCCTACCAGATT+TGG 0.288300 1_4:-16125633 Msa0153040:CDS
CTCCTTTGATTTCCTGGATT+CGG 0.304226 1_4:-16127929 Msa0153040:CDS
TATGCATCCCCTATATAAAT+AGG 0.310663 1_4:+16125746 None:intergenic
CTTCAAATTTGCTCCAAATC+TGG 0.314432 1_4:+16125620 None:intergenic
CCAACAAAGACAAGAAAATA+AGG 0.331796 1_4:-16127824 Msa0153040:CDS
TCTTGCAGACGCACGTTCAT+AGG 0.341018 1_4:+16125869 None:intergenic
GCGAGCCTCCTATTTATATA+GGG 0.371848 1_4:-16125754 Msa0153040:CDS
TTACACAAGAGAACGGTTTA+GGG 0.383205 1_4:-16125488 Msa0153040:CDS
TGCGAGCCTCCTATTTATAT+AGG 0.401271 1_4:-16125755 Msa0153040:CDS
TTTATATAGGGGATGCATAA+TGG 0.404335 1_4:-16125742 Msa0153040:intron
CAAGGATTCTGCTGGATGTA+TGG 0.411184 1_4:-16127875 Msa0153040:CDS
CTCTTACTTCTGAGTGATCC+TGG 0.418604 1_4:-16125292 Msa0153040:three_prime_UTR
CAAGCTATGTTGCATAGGTA+TGG 0.423542 1_4:+16124923 None:intergenic
CAAGAAAACTTCAAGAAAGA+AGG 0.440243 1_4:-16126244 Msa0153040:CDS
TACTTTGTTTCGTCTAAAAG+AGG 0.450484 1_4:-16125409 Msa0153040:CDS
GTGTAAATTCGTACCATCCT+TGG 0.456711 1_4:+16125505 None:intergenic
CATAGAAAGGCTCTTCAGTC+CGG 0.462587 1_4:+16127992 None:intergenic
GGTTTGAAGGTGCCGAATCC+AGG 0.469504 1_4:+16127917 None:intergenic
AATGCACCAGTAGGGTTTGA+AGG 0.471364 1_4:+16127904 None:intergenic
GATACTTTCTATGTCCATAC+CGG 0.474003 1_4:-16125239 Msa0153040:three_prime_UTR
CAGATTTGGAGCAAATTTGA+AGG 0.476313 1_4:-16125619 Msa0153040:CDS
GTCCATACCGGGTACGCGTA+CGG 0.483203 1_4:-16125227 Msa0153040:three_prime_UTR
TGCTTTGCAATGCACCAGTA+GGG 0.484811 1_4:+16127896 None:intergenic
TTATATAATATAACATGATA+GGG 0.495380 1_4:+16125021 None:intergenic
TGGGTACAGGTACGAGATTA+CGG 0.497721 1_4:+16124943 None:intergenic
AACTTGCAACACTACATCTC+TGG 0.504545 1_4:+16125431 None:intergenic
AGAGAACGGTTTAGGGGAAG+GGG 0.514068 1_4:-16125481 Msa0153040:CDS
TTGTGGAACTGCAGGTGCAC+AGG 0.514227 1_4:-16126313 Msa0153040:intron
TTGTGGAACTGCAGGTGCAC+AGG 0.514227 1_4:-16127494 Msa0153040:intron
TGTCAGATGAAGAAAGAGAA+AGG 0.518068 1_4:-16125833 Msa0153040:CDS
TACCGTACGCGTACCCGGTA+TGG 0.521278 1_4:+16125225 None:intergenic
CTAACGCAGTGCTGCAAGTG+AGG 0.521510 1_4:+16125380 None:intergenic
ACAAGACAGTAAGGATTAGT+TGG 0.522215 1_4:+16125783 None:intergenic
TAATTATAGTCTTCATAGAA+AGG 0.526871 1_4:+16127979 None:intergenic
AAGAAAACTTCAAGAAAGAA+GGG 0.528573 1_4:-16126243 Msa0153040:CDS
AAGAGAACGGTTTAGGGGAA+GGG 0.530709 1_4:-16125482 Msa0153040:CDS
TTGCAAAGCAAGGATTCTGC+TGG 0.532722 1_4:-16127883 Msa0153040:CDS
CAATCCAAGCTATGTTGCAT+AGG 0.534379 1_4:+16124918 None:intergenic
TTGCTTTGCAATGCACCAGT+AGG 0.535055 1_4:+16127895 None:intergenic
CAAGAGAACGGTTTAGGGGA+AGG 0.536429 1_4:-16125483 Msa0153040:CDS
TGTTGGCTATGATTCTCCGC+TGG 0.543021 1_4:+16127841 None:intergenic
AGATTTGGAGCAAATTTGAA+GGG 0.548691 1_4:-16125618 Msa0153040:CDS
TCGGCACCTTCAAACCCTAC+TGG 0.554321 1_4:-16127910 Msa0153040:CDS
GTCAGATGAAGAAAGAGAAA+GGG 0.559696 1_4:-16125832 Msa0153040:CDS
AAGAGAAAGGGCAATGAATG+AGG 0.560927 1_4:-16125820 Msa0153040:CDS
CGCAGACTAACAAGACAGTA+AGG 0.562349 1_4:+16125774 None:intergenic
GCTGCCTTGATGACAAAGGC+CGG 0.563135 1_4:-16125539 Msa0153040:CDS
ATGATTCAACAATGAGAAGC+CGG 0.564717 1_4:-16128011 None:intergenic
AAGCTATGTTGCATAGGTAT+GGG 0.564739 1_4:+16124924 None:intergenic
TGTTGCATAGGTATGGGTAC+AGG 0.576281 1_4:+16124930 None:intergenic
ACGCCACTGTTTCTCACCAT+CGG 0.581074 1_4:+16125566 None:intergenic
ATACTTTCTATGTCCATACC+GGG 0.587476 1_4:-16125238 Msa0153040:three_prime_UTR
ATTCCGCTTCAGACATCCGA+TGG 0.591154 1_4:-16125582 Msa0153040:CDS
CAAGAAAGAAGCGGAAATCG+CGG 0.592113 1_4:-16127786 Msa0153040:CDS
TACTGGTGCATTGCAAAGCA+AGG 0.593014 1_4:-16127893 Msa0153040:CDS
AAATTTGCTCCAAATCTGGT+AGG 0.596789 1_4:+16125624 None:intergenic
CGAGCCTCCTATTTATATAG+GGG 0.597293 1_4:-16125753 Msa0153040:CDS
TACGAATTTACACAAGAGAA+CGG 0.605728 1_4:-16125495 Msa0153040:CDS
CTCCACTGAAAATGATGAGG+AGG 0.606101 1_4:-16126286 Msa0153040:CDS
TGAATAAACTAGAGAATACC+AGG 0.606340 1_4:+16125274 None:intergenic
TAACGCAGTGCTGCAAGTGA+GGG 0.609733 1_4:+16125381 None:intergenic
AGAAAACTTCAAGAAAGAAG+CGG 0.610439 1_4:-16127423 Msa0153040:CDS
AGAAAACTTCAAGAAAGAAG+CGG 0.610439 1_4:-16127795 Msa0153040:CDS
TGCCGAATCCAGGAAATCAA+AGG 0.612101 1_4:+16127927 None:intergenic
ACGAGTACCGTACGCGTACC+CGG 0.614617 1_4:+16125220 None:intergenic
GCATCCCCTATATAAATAGG+AGG 0.614656 1_4:+16125749 None:intergenic
TCACCATCGGATGTCTGAAG+CGG 0.621327 1_4:+16125579 None:intergenic
CATCCGATGGTGAGAAACAG+TGG 0.644228 1_4:-16125569 Msa0153040:CDS
AGCCTCCACTGAAAATGATG+AGG 0.645254 1_4:-16126289 Msa0153040:CDS
GTTCGCTGCCTTGATGACAA+AGG 0.645710 1_4:-16125543 Msa0153040:CDS
TACACAAGAGAACGGTTTAG+GGG 0.668422 1_4:-16125487 Msa0153040:CDS
CTCCACTGAAAATGACGAGG+AGG 0.686073 1_4:-16127467 Msa0153040:CDS
AGCCTCCACTGAAAATGACG+AGG 0.698578 1_4:-16127470 Msa0153040:CDS
TATGGATCAACTGATACCAG+CGG 0.714314 1_4:-16127857 Msa0153040:CDS
GGGTACGCGTACGGTACTCG+TGG 0.714655 1_4:-16125218 Msa0153040:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAAATAGTTTAAAATG+TGG + chr1_4:16125851-16125870 None:intergenic 10.0%
!!! ACATTTTAAACTATTTTTTT+TGG - chr1_4:16125850-16125869 Msa0153040:CDS 10.0%
!!! AATTTTTTGATATTTTTCTT+TGG - chr1_4:16127227-16127246 Msa0153040:intron 10.0%
!! TTATATAATATAACATGATA+GGG + chr1_4:16127913-16127932 None:intergenic 10.0%
!!! TTTTTTTAAAATTAAGATAC+GGG + chr1_4:16127965-16127984 None:intergenic 10.0%
!! TTGAAGTTTGAATAAAAATA+TGG - chr1_4:16126445-16126464 Msa0153040:intron 15.0%
!! AAAAAGTTGAAAATTAAAGT+TGG + chr1_4:16127847-16127866 None:intergenic 15.0%
!! GTTATATAATATAACATGAT+AGG + chr1_4:16127914-16127933 None:intergenic 15.0%
!! TAATTATAGTCTTCATAGAA+AGG + chr1_4:16124955-16124974 None:intergenic 20.0%
!!! GACTATAATTATGTTTTTGA+AGG - chr1_4:16124964-16124983 Msa0153040:three_prime_UTR 20.0%
!!! TATTTCACGTTTTCAAATTA+CGG - chr1_4:16125316-16125335 Msa0153040:three_prime_UTR 20.0%
!! ATTGTCATCAATATAATTTG+TGG - chr1_4:16125420-16125439 Msa0153040:CDS 20.0%
!! TGTATAATTAAGAAAGTTGT+TGG + chr1_4:16126011-16126030 None:intergenic 20.0%
!! TTAATTATACAGAGAAAGAA+TGG - chr1_4:16126020-16126039 Msa0153040:intron 20.0%
!! CAAATTTGATTACTGAAAAA+TGG + chr1_4:16126179-16126198 None:intergenic 20.0%
!!! CATTTTTCAGTAATCAAATT+TGG - chr1_4:16126177-16126196 Msa0153040:intron 20.0%
!! ATTGCTTCTGATAAAAAAAT+TGG + chr1_4:16126315-16126334 None:intergenic 20.0%
!! ATTGTCATCAATATAATTTG+TGG - chr1_4:16125420-16125439 Msa0153040:CDS 20.0%
!!! TATTTCACGTTTTCAAATTA+TGG - chr1_4:16125316-16125335 Msa0153040:three_prime_UTR 20.0%
!! CATAGAATAAATAGCAAAAA+TGG + chr1_4:16127743-16127762 None:intergenic 20.0%
!!! CATTTTTGCTATTTATTCTA+TGG - chr1_4:16127741-16127760 Msa0153040:intron 20.0%
! TTTACTATACTATGTAGCAT+TGG - chr1_4:16125207-16125226 Msa0153040:three_prime_UTR 25.0%
!! TATTTCACGTTTTCAAACTA+CGG - chr1_4:16125679-16125698 Msa0153040:intron 25.0%
!!! AAACTATTTTTTTTGGCTAC+CGG - chr1_4:16125857-16125876 Msa0153040:CDS 25.0%
! TGTGCTCTATTTATTTGATT+TGG + chr1_4:16126148-16126167 None:intergenic 25.0%
! TCAGTAATCAAATTTGGATT+TGG - chr1_4:16126183-16126202 Msa0153040:intron 25.0%
!!! GTTTGAGATTAATGTTCTTT+TGG - chr1_4:16126365-16126384 Msa0153040:intron 25.0%
! AAAACTAAAAACCTGATAAC+CGG + chr1_4:16126494-16126513 None:intergenic 25.0%
!!! GTTTTAAAAAAGTGATGATG+TGG - chr1_4:16126509-16126528 Msa0153040:intron 25.0%
! TCAAACACTCTTTCAATTAT+TGG + chr1_4:16126536-16126555 None:intergenic 25.0%
! AAGAAAACTTCAAGAAAGAA+GGG - chr1_4:16125135-16125154 Msa0153040:three_prime_UTR 25.0%
! ATTGTCATCAGAATAATTTG+TGG - chr1_4:16126965-16126984 Msa0153040:intron 25.0%
!! ATTCTCTAGTTTATTCAGTT+TGG - chr1_4:16127663-16127682 Msa0153040:intron 25.0%
!!! CCTTATTTTCTTGTCTTTGT+TGG + chr1_4:16125110-16125129 None:intergenic 30.0%
CCAACAAAGACAAGAAAATA+AGG - chr1_4:16125107-16125126 Msa0153040:three_prime_UTR 30.0%
! CTTCTTTCTTGAAGTTTTCT+TGG + chr1_4:16125136-16125155 None:intergenic 30.0%
AGAAAACTTCAAGAAAGAAG+CGG - chr1_4:16125136-16125155 Msa0153040:three_prime_UTR 30.0%
! AATATAATTTGTGGAACTGC+AGG - chr1_4:16125429-16125448 Msa0153040:CDS 30.0%
! CTTCTTTCTTGAAGTTTTCT+TGG + chr1_4:16125136-16125155 None:intergenic 30.0%
AGAAAACTTCAAGAAAGAAG+CGG - chr1_4:16125136-16125155 Msa0153040:three_prime_UTR 30.0%
! AGTTTAAAATGTGGATGACT+TGG + chr1_4:16125842-16125861 None:intergenic 30.0%
TCACTGTGTAAGTAAATAAC+CGG + chr1_4:16125879-16125898 None:intergenic 30.0%
! ATTAAGAAAGTTGTTGGTTG+TGG + chr1_4:16126005-16126024 None:intergenic 30.0%
TTTGTGATGTTGAAGCTTAA+TGG + chr1_4:16126069-16126088 None:intergenic 30.0%
AACATCACAAACTTGTCAAA+TGG - chr1_4:16126078-16126097 Msa0153040:intron 30.0%
ACATCACAAACTTGTCAAAT+GGG - chr1_4:16126079-16126098 Msa0153040:intron 30.0%
!!! GCTGTGATATAGGTTTTTTA+TGG - chr1_4:16126240-16126259 Msa0153040:CDS 30.0%
GCAGATGAGATAATTAAAGA+TGG - chr1_4:16126262-16126281 Msa0153040:CDS 30.0%
! ATTAGTTTCATCATGAGAGA+TGG - chr1_4:16126392-16126411 Msa0153040:intron 30.0%
! AATATAATTTGTGGAACTGC+AGG - chr1_4:16125429-16125448 Msa0153040:CDS 30.0%
! CTTCTTTCTTGAAGTTTTCT+TGG + chr1_4:16125136-16125155 None:intergenic 30.0%
CAAGAAAACTTCAAGAAAGA+AGG - chr1_4:16125134-16125153 Msa0153040:three_prime_UTR 30.0%
TATGCATCCCCTATATAAAT+AGG + chr1_4:16127188-16127207 None:intergenic 30.0%
TTTATATAGGGGATGCATAA+TGG - chr1_4:16127189-16127208 Msa0153040:intron 30.0%
! AGATTTGGAGCAAATTTGAA+GGG - chr1_4:16127313-16127332 Msa0153040:intron 30.0%
TACGAATTTACACAAGAGAA+CGG - chr1_4:16127436-16127455 Msa0153040:CDS 30.0%
! TACTTTGTTTCGTCTAAAAG+AGG - chr1_4:16127522-16127541 Msa0153040:intron 30.0%
!! CAGTTTTGTAATTGTCTATC+AGG + chr1_4:16127609-16127628 None:intergenic 30.0%
TGAATAAACTAGAGAATACC+AGG + chr1_4:16127660-16127679 None:intergenic 30.0%
! GCATTGACATTTCAGATTGA+AGG - chr1_4:16125223-16125242 Msa0153040:three_prime_UTR 35.0%
!! GGAGGCTTTTGGAAAATTAA+AGG + chr1_4:16126048-16126067 None:intergenic 35.0%
TTCAAAGAACCTGCGTTAAT+TGG - chr1_4:16126105-16126124 Msa0153040:intron 35.0%
TCAAAGAACCTGCGTTAATT+GGG - chr1_4:16126106-16126125 Msa0153040:intron 35.0%
!! AAGCAAACTTGCTGTGATAT+AGG - chr1_4:16126230-16126249 Msa0153040:CDS 35.0%
!! GTTTTGTTGAGATTCGATTG+TGG - chr1_4:16126341-16126360 Msa0153040:intron 35.0%
CTCTCATGATGAAACTAATG+AGG + chr1_4:16126391-16126410 None:intergenic 35.0%
TGTTAGTAAACCAGTATAGC+CGG - chr1_4:16126472-16126491 Msa0153040:intron 35.0%
! GCATTGACATTTCAGATTGA+AGG - chr1_4:16125223-16125242 Msa0153040:three_prime_UTR 35.0%
! TCACGTTTTCAAATTATGGC+TGG - chr1_4:16126873-16126892 Msa0153040:intron 35.0%
! AGAATAATTTGTGGAACTGC+AGG - chr1_4:16126974-16126993 Msa0153040:intron 35.0%
TGTCAGATGAAGAAAGAGAA+AGG - chr1_4:16127098-16127117 Msa0153040:intron 35.0%
GTCAGATGAAGAAAGAGAAA+GGG - chr1_4:16127099-16127118 Msa0153040:intron 35.0%
! ACAAGACAGTAAGGATTAGT+TGG + chr1_4:16127151-16127170 None:intergenic 35.0%
! AAATTTGCTCCAAATCTGGT+AGG + chr1_4:16127310-16127329 None:intergenic 35.0%
! CTTCAAATTTGCTCCAAATC+TGG + chr1_4:16127314-16127333 None:intergenic 35.0%
! CAGATTTGGAGCAAATTTGA+AGG - chr1_4:16127312-16127331 Msa0153040:intron 35.0%
!! ATCCTTGGCTTAACTTTTTC+CGG + chr1_4:16127414-16127433 None:intergenic 35.0%
TTTACACAAGAGAACGGTTT+AGG - chr1_4:16127442-16127461 Msa0153040:CDS 35.0%
TTACACAAGAGAACGGTTTA+GGG - chr1_4:16127443-16127462 Msa0153040:CDS 35.0%
AGCTCAACCTAAAAATTAGC+TGG - chr1_4:16127572-16127591 Msa0153040:intron 35.0%
GCTCAACCTAAAAATTAGCT+GGG - chr1_4:16127573-16127592 Msa0153040:intron 35.0%
! AACTCTCCCAGCTAATTTTT+AGG + chr1_4:16127582-16127601 None:intergenic 35.0%
GATACTTTCTATGTCCATAC+CGG - chr1_4:16127692-16127711 Msa0153040:intron 35.0%
ATACTTTCTATGTCCATACC+GGG - chr1_4:16127693-16127712 Msa0153040:intron 35.0%
!! TATCGACTCCTTTGATTTCC+TGG - chr1_4:16124996-16125015 Msa0153040:three_prime_UTR 40.0%
! CTCCTTTGATTTCCTGGATT+CGG - chr1_4:16125002-16125021 Msa0153040:three_prime_UTR 40.0%
TATGGATCAACTGATACCAG+CGG - chr1_4:16125074-16125093 Msa0153040:three_prime_UTR 40.0%
! TCACGTTTTCAAATTACGGC+TGG - chr1_4:16125320-16125339 Msa0153040:three_prime_UTR 40.0%
! GCATTGGCATTTCAGATTGA+AGG - chr1_4:16125586-16125605 Msa0153040:CDS 40.0%
CTCAAAGAGAGAGTGGTTAT+TGG + chr1_4:16125815-16125834 None:intergenic 40.0%
ATAACCACTCTCTCTTTGAG+TGG - chr1_4:16125815-16125834 Msa0153040:CDS 40.0%
ACGTTCTCTTTCTCTCTCTT+TGG - chr1_4:16125939-16125958 Msa0153040:intron 40.0%
GAAGACCTTTCAAATGACAG+AGG + chr1_4:16125970-16125989 None:intergenic 40.0%
! TTGTTGGTTGTGGTTAGATG+GGG + chr1_4:16125995-16126014 None:intergenic 40.0%
! GTTGTTGGTTGTGGTTAGAT+GGG + chr1_4:16125996-16126015 None:intergenic 40.0%
! AGTTGTTGGTTGTGGTTAGA+TGG + chr1_4:16125997-16126016 None:intergenic 40.0%
! TGAAGCTTAATGGAGGCTTT+TGG + chr1_4:16126059-16126078 None:intergenic 40.0%
GTGATGTTGAAGCTTAATGG+AGG + chr1_4:16126066-16126085 None:intergenic 40.0%
AGATGAATCCCAATTAACGC+AGG + chr1_4:16126117-16126136 None:intergenic 40.0%
TGCGTTAATTGGGATTCATC+TGG - chr1_4:16126116-16126135 Msa0153040:intron 40.0%
TTTGAGAGAGAGTGATAGAG+AGG - chr1_4:16126551-16126570 Msa0153040:intron 40.0%
AAGAGAAAGGGCAATGAATG+AGG - chr1_4:16127111-16127130 Msa0153040:intron 40.0%
TGCGAGCCTCCTATTTATAT+AGG - chr1_4:16127176-16127195 Msa0153040:intron 40.0%
GCGAGCCTCCTATTTATATA+GGG - chr1_4:16127177-16127196 Msa0153040:intron 40.0%
CGAGCCTCCTATTTATATAG+GGG - chr1_4:16127178-16127197 Msa0153040:intron 40.0%
GCATCCCCTATATAAATAGG+AGG + chr1_4:16127185-16127204 None:intergenic 40.0%
CAAATCTGGTAGGCAAATAC+TGG + chr1_4:16127300-16127319 None:intergenic 40.0%
CAGTATTTGCCTACCAGATT+TGG - chr1_4:16127298-16127317 Msa0153040:intron 40.0%
!! GCAAATTTGAAGGGTGTTTC+TGG - chr1_4:16127322-16127341 Msa0153040:intron 40.0%
GGAAAAAGTTAAGCCAAGGA+TGG - chr1_4:16127413-16127432 Msa0153040:CDS 40.0%
GTGTAAATTCGTACCATCCT+TGG + chr1_4:16127429-16127448 None:intergenic 40.0%
TACACAAGAGAACGGTTTAG+GGG - chr1_4:16127444-16127463 Msa0153040:CDS 40.0%
AACTTGCAACACTACATCTC+TGG + chr1_4:16127503-16127522 None:intergenic 40.0%
CATAGAAAGGCTCTTCAGTC+CGG + chr1_4:16124942-16124961 None:intergenic 45.0%
TGCCGAATCCAGGAAATCAA+AGG + chr1_4:16125007-16125026 None:intergenic 45.0%
AATGCACCAGTAGGGTTTGA+AGG + chr1_4:16125030-16125049 None:intergenic 45.0%
TGCTTTGCAATGCACCAGTA+GGG + chr1_4:16125038-16125057 None:intergenic 45.0%
TTGCTTTGCAATGCACCAGT+AGG + chr1_4:16125039-16125058 None:intergenic 45.0%
TACTGGTGCATTGCAAAGCA+AGG - chr1_4:16125038-16125057 Msa0153040:three_prime_UTR 45.0%
TTGCAAAGCAAGGATTCTGC+TGG - chr1_4:16125048-16125067 Msa0153040:three_prime_UTR 45.0%
CAAGGATTCTGCTGGATGTA+TGG - chr1_4:16125056-16125075 Msa0153040:three_prime_UTR 45.0%
CAAGAAAGAAGCGGAAATCG+CGG - chr1_4:16125145-16125164 Msa0153040:three_prime_UTR 45.0%
! TTTCAGATTGAAGGCATGCC+CGG - chr1_4:16125232-16125251 Msa0153040:three_prime_UTR 45.0%
ATTGATGTGTGTCAGACACC+GGG + chr1_4:16125253-16125272 None:intergenic 45.0%
CATTGATGTGTGTCAGACAC+CGG + chr1_4:16125254-16125273 None:intergenic 45.0%
! TTTCAGATTGAAGGCATGCC+CGG - chr1_4:16125232-16125251 Msa0153040:three_prime_UTR 45.0%
ATTGATGTGTGTCAGACACC+GGG + chr1_4:16125253-16125272 None:intergenic 45.0%
CATTGATGTGTGTCAGACAC+CGG + chr1_4:16125254-16125273 None:intergenic 45.0%
TGTTAGTGTCGCATCTGAGT+TGG + chr1_4:16125641-16125660 None:intergenic 45.0%
! TCACGTTTTCAAACTACGGC+TGG - chr1_4:16125683-16125702 Msa0153040:intron 45.0%
! CACCTCCTCTGTCATTTGAA+AGG - chr1_4:16125962-16125981 Msa0153040:intron 45.0%
GACCTTTCAAATGACAGAGG+AGG + chr1_4:16125967-16125986 None:intergenic 45.0%
AACCAGTATAGCCGGTTATC+AGG - chr1_4:16126480-16126499 Msa0153040:intron 45.0%
AACCTGATAACCGGCTATAC+TGG + chr1_4:16126485-16126504 None:intergenic 45.0%
AGCCTCCACTGAAAATGATG+AGG - chr1_4:16126642-16126661 Msa0153040:intron 45.0%
! CTCCTCATCATTTTCAGTGG+AGG + chr1_4:16126647-16126666 None:intergenic 45.0%
CTCCACTGAAAATGATGAGG+AGG - chr1_4:16126645-16126664 Msa0153040:intron 45.0%
! TGCCTCCTCATCATTTTCAG+TGG + chr1_4:16126650-16126669 None:intergenic 45.0%
! TTTCAGATTGAAGGCATGCC+CGG - chr1_4:16125232-16125251 Msa0153040:three_prime_UTR 45.0%
ATTGATGTGTGTCAGACACC+GGG + chr1_4:16125253-16125272 None:intergenic 45.0%
CATTGATGTGTGTCAGACAC+CGG + chr1_4:16125254-16125273 None:intergenic 45.0%
CTGGTATCAATGTCTCAGTG+CGG - chr1_4:16126892-16126911 Msa0153040:intron 45.0%
CGCAGACTAACAAGACAGTA+AGG + chr1_4:16127160-16127179 None:intergenic 45.0%
! TTTTCCGGCCTTTGTCATCA+AGG + chr1_4:16127399-16127418 None:intergenic 45.0%
AAGAGAACGGTTTAGGGGAA+GGG - chr1_4:16127449-16127468 Msa0153040:CDS 45.0%
CTCTTACTTCTGAGTGATCC+TGG - chr1_4:16127639-16127658 Msa0153040:intron 45.0%
TGGGTACAGGTACGAGATTA+CGG + chr1_4:16127991-16128010 None:intergenic 45.0%
!!! ATTTTTTTAAAATTAAGATA+CGG + chr1_4:16127966-16127985 None:intergenic 5.0%
! TGTTGGCTATGATTCTCCGC+TGG + chr1_4:16125093-16125112 None:intergenic 50.0%
AGCCTCCACTGAAAATGACG+AGG - chr1_4:16125461-16125480 Msa0153040:CDS 50.0%
!! CTCCTCGTCATTTTCAGTGG+AGG + chr1_4:16125466-16125485 None:intergenic 50.0%
CTCCACTGAAAATGACGAGG+AGG - chr1_4:16125464-16125483 Msa0153040:CDS 50.0%
!! TGCCTCCTCGTCATTTTCAG+TGG + chr1_4:16125469-16125488 None:intergenic 50.0%
AGATGCGACACTAACACGTG+TGG - chr1_4:16125645-16125664 Msa0153040:intron 50.0%
CAGACACGACACTGAGACAT+TGG + chr1_4:16125711-16125730 None:intergenic 50.0%
ATTAGAGCAGTGCTAGCGTC+AGG - chr1_4:16125775-16125794 Msa0153040:CDS 50.0%
TGGTCCACTCAAAGAGAGAG+TGG + chr1_4:16125822-16125841 None:intergenic 50.0%
TCTTGCAGACGCACGTTCAT+AGG + chr1_4:16127065-16127084 None:intergenic 50.0%
ATTCCGCTTCAGACATCCGA+TGG - chr1_4:16127349-16127368 Msa0153040:intron 50.0%
TCACCATCGGATGTCTGAAG+CGG + chr1_4:16127355-16127374 None:intergenic 50.0%
CATCCGATGGTGAGAAACAG+TGG - chr1_4:16127362-16127381 Msa0153040:intron 50.0%
! ACGCCACTGTTTCTCACCAT+CGG + chr1_4:16127368-16127387 None:intergenic 50.0%
! GTTCGCTGCCTTGATGACAA+AGG - chr1_4:16127388-16127407 Msa0153040:intron 50.0%
GGCCGGAAAAAGTTAAGCCA+AGG - chr1_4:16127409-16127428 Msa0153040:intron 50.0%
CAAGAGAACGGTTTAGGGGA+AGG - chr1_4:16127448-16127467 Msa0153040:CDS 50.0%
AGAGAACGGTTTAGGGGAAG+GGG - chr1_4:16127450-16127469 Msa0153040:CDS 50.0%
TAACGCAGTGCTGCAAGTGA+GGG + chr1_4:16127553-16127572 None:intergenic 50.0%
!! GGTTTGAAGGTGCCGAATCC+AGG + chr1_4:16125017-16125036 None:intergenic 55.0%
TCGGCACCTTCAAACCCTAC+TGG - chr1_4:16125021-16125040 Msa0153040:three_prime_UTR 55.0%
TGTCAGTGTCGCGTCTGAGT+TGG + chr1_4:16125278-16125297 None:intergenic 55.0%
AGACGCGACACTGACATGTG+TGG - chr1_4:16125282-16125301 Msa0153040:three_prime_UTR 55.0%
CAGACACGACACTGAGACGT+TGG + chr1_4:16125348-16125367 None:intergenic 55.0%
TTGTGGAACTGCAGGTGCAC+AGG - chr1_4:16125437-16125456 Msa0153040:CDS 55.0%
TTGTGGAACTGCAGGTGCAC+AGG - chr1_4:16125437-16125456 Msa0153040:CDS 55.0%
AGACGCGACACTAACACGTG+TGG - chr1_4:16126835-16126854 Msa0153040:intron 55.0%
! GCTGCCTTGATGACAAAGGC+CGG - chr1_4:16127392-16127411 Msa0153040:intron 55.0%
CTAACGCAGTGCTGCAAGTG+AGG + chr1_4:16127554-16127573 None:intergenic 55.0%
GTCCATACCGGGTACGCGTA+CGG - chr1_4:16127704-16127723 Msa0153040:intron 60.0%
TACCGTACGCGTACCCGGTA+TGG + chr1_4:16127709-16127728 None:intergenic 60.0%
! ACGAGTACCGTACGCGTACC+CGG + chr1_4:16127714-16127733 None:intergenic 60.0%
GGGTACGCGTACGGTACTCG+TGG - chr1_4:16127713-16127732 Msa0153040:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 16124931 16128022 16124931 ID=Msa0153040;Name=Msa0153040
chr1_4 mRNA 16124931 16128022 16124931 ID=Msa0153040-mRNA-1;Parent=Msa0153040;Name=Msa0153040-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|1|0.8|1|1|1|5|449|310
chr1_4 exon 16124931 16125652 16124931 ID=Msa0153040-mRNA-1:exon:6213;Parent=Msa0153040-mRNA-1
chr1_4 exon 16125743 16125957 16125743 ID=Msa0153040-mRNA-1:exon:6212;Parent=Msa0153040-mRNA-1
chr1_4 exon 16126220 16126321 16126220 ID=Msa0153040-mRNA-1:exon:6211;Parent=Msa0153040-mRNA-1
chr1_4 exon 16127410 16127502 16127410 ID=Msa0153040-mRNA-1:exon:6210;Parent=Msa0153040-mRNA-1
chr1_4 exon 16127773 16128022 16127773 ID=Msa0153040-mRNA-1:exon:6209;Parent=Msa0153040-mRNA-1
chr1_4 CDS 16127773 16128022 16127773 ID=Msa0153040-mRNA-1:cds;Parent=Msa0153040-mRNA-1
chr1_4 CDS 16127410 16127502 16127410 ID=Msa0153040-mRNA-1:cds;Parent=Msa0153040-mRNA-1
chr1_4 CDS 16126220 16126321 16126220 ID=Msa0153040-mRNA-1:cds;Parent=Msa0153040-mRNA-1
chr1_4 CDS 16125743 16125957 16125743 ID=Msa0153040-mRNA-1:cds;Parent=Msa0153040-mRNA-1
chr1_4 CDS 16125380 16125652 16125380 ID=Msa0153040-mRNA-1:cds;Parent=Msa0153040-mRNA-1
chr1_4 three_prime_UTR 16124931 16125379 16124931 ID=Msa0153040-mRNA-1:three_prime_utr;Parent=Msa0153040-mRNA-1
Gene Sequence

>Msa0153040

ATGAGAAGCCGGACTGAAGAGCCTTTCTATGAAGACTATAATTATGTTTTTGAAGGCATTGAAGATATCGACTCCTTTGATTTCCTGGATTCGGCACCTTCAAACCCTACTGGTGCATTGCAAAGCAAGGATTCTGCTGGATGTATGGATCAACTGATACCAGCGGAGAATCATAGCCAACAAAGACAAGAAAATAAGGATACCAAGAAAACTTCAAGAAAGAAGCGGAAATCGCGGAAATCAGATCTGAGTGCACAGGAAGCCTCCACTGAAAATGACGAGGAGGCAGAGCGCAATAGAGATACCAAGAAAACTTCAAGAAAGAAGCGGAAATCAGATCTGAGTGCACAGGAAGCCTCCACTGAAAATGATGAGGAGGCAGAGCGCAATCTAGTTACCAAGAAAACTTCAAGAAAGAAGGGAAAATCGCAGAAATCAGATCTGAGTGCACAGCGCAATCGATCTTATGAAAGTGCGTCTGCAAGAGTAAGAAGCAACATGTCAAATGTAGCCTATGAACGTGCGTCTGCAAGAGTAAGACACAATGTGTCAGATGAAGAAAGAGAAAGGGCAATGAATGAGGCAAAAGCATTTGTACCAACTAATCCTTACTGTCTTGTTAGTCTGCGAGCCTCCTATTTATATAGGGGATGCATAATGTATTTGCCTACCAGATTTGGAGCAAATTTGAAGGGTGTTTCTGGTTTTATTCCGCTTCAGACATCCGATGGTGAGAAACAGTGGCGTGTTCGCTGCCTTGATGACAAAGGCCGGAAAAAGTTAAGCCAAGGATGGTACGAATTTACACAAGAGAACGGTTTAGGGGAAGGGGATATCTGTGTTTTTGAGCTGCTTAATACCAGAGATGTAGTGTTGCAAGTTACTTTGTTTCGTCTAAAAGAGGATGAACCCTCACTTGCAGCACTGCGTTAG
Protein sequence

>Msa0153040

MRSRTEEPFYEDYNYVFEGIEDIDSFDFLDSAPSNPTGALQSKDSAGCMDQLIPAENHSQQRQENKDTKKTSRKKRKSRKSDLSAQEASTENDEEAERNRDTKKTSRKKRKSDLSAQEASTENDEEAERNLVTKKTSRKKGKSQKSDLSAQRNRSYESASARVRSNMSNVAYERASARVRHNVSDEERERAMNEAKAFVPTNPYCLVSLRASYLYRGCIMYLPTRFGANLKGVSGFIPLQTSDGEKQWRVRCLDDKGRKKLSQGWYEFTQENGLGEGDICVFELLNTRDVVLQVTLFRLKEDEPSLAALR*