AlfalfaGEDB | Msa0153530

M. sativa cultivar zhongmu-4 / Msa0153530

KEGG: -
GO: GO:0000902 GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0005975 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006884 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0008361 GO:0009311 GO:0009505 GO:0009605 GO:0009607 GO:0009615 GO:0009620 GO:0009653 GO:0009719 GO:0009725 GO:0009751 GO:0009826 GO:0009987 GO:0009992 GO:0010033 GO:0014070 GO:0016020 GO:0016043 GO:0016049 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019725 GO:0023052 GO:0030104 GO:0030312 GO:0032502 GO:0032535 GO:0032989 GO:0036211 GO:0040007 GO:0042221 GO:0042493 GO:0042592 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046677 GO:0048589 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0055082 GO:0060560 GO:0065007 GO:0065008 GO:0071704 GO:0071840 GO:0071944 GO:0090066 GO:0098542 GO:0140096 GO:1901564 GO:1901700
PFAM: PF13947.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0153530	sp\|Q9LMN8\|WAK3_ARATH	40.891	741	344	19	29	705	31	741	2.61e-165	496

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0153530	A0A396JI89	77.237	760	110	10	1	705	4	755	0.0	1156

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0153530	MtrunA17_Chr1g0148511	77.237	760	110	10	1	705	4	755	1156
Msa0153530	MtrunA17_Chr1g0148491	76.618	680	99	11	75	705	1	669	1009
Msa0153530	MtrunA17_Chr1g0148501	57.827	773	246	12	1	705	1	761	853
Msa0153530	MtrunA17_Chr1g0158201	59.003	722	237	10	29	705	37	744	822
Msa0153530	MtrunA17_Chr1g0158261	56.782	752	254	15	5	705	9	740	812
Msa0153530	MtrunA17_Chr1g0158251	54.161	757	281	13	2	705	6	749	775
Msa0153530	MtrunA17_Chr1g0148461	57.931	725	218	14	13	668	25	731	774
Msa0153530	MtrunA17_Chr1g0158211	62.955	467	123	5	280	705	25	482	569
Msa0153530	MtrunA17_Chr1g0158231	62.099	467	127	5	280	705	3	460	558
Msa0153530	MtrunA17_Chr3g0125431	44.293	736	320	17	9	674	10	725	554

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0153530	AT1G21240.1	40.891	741	344	19	29	705	31	741	2.66e-166	496
Msa0153530	AT1G21270.1	40.162	742	359	18	20	705	20	732	4.53e-164	490
Msa0153530	AT1G21230.1	40.079	761	372	17	1	705	1	733	8.53e-164	489
Msa0153530	AT1G21250.1	40.327	734	351	18	29	705	32	735	1.79e-162	486
Msa0153530	AT1G21210.1	38.312	770	378	23	1	705	1	738	1.10e-151	458
Msa0153530	AT1G16130.1	36.857	719	362	21	4	657	8	699	1.41e-130	404
Msa0153530	AT1G16120.1	38.075	696	332	20	25	648	35	703	1.71e-125	390
Msa0153530	AT1G79670.1	36.726	727	349	23	1	648	1	695	8.15e-125	389
Msa0153530	AT1G16150.1	36.426	733	361	24	4	658	3	708	3.38e-124	388
Msa0153530	AT1G16260.1	35.223	741	349	23	24	689	28	712	4.81e-119	373

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Find 144 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGTGATGTCTATAGCTT+TGG	0.393895	1_4:-16910640	Msa0153530:CDS
AAATAAACAAGAGATCAAGG+AGG	0.787103	1_4:-16910470	Msa0153530:CDS
AAATGCAGTCACACTTGTGG+AGG	0.623911	1_4:-16913142	Msa0153530:CDS
AAATGCATAGCTACAAAATT+CGG	0.390893	1_4:+16912652	None:intergenic
AAATTCTTTGAGCAGAATGG+TGG	0.605947	1_4:-16911322	Msa0153530:CDS
AAATTGACCTGTGCAGCCAT+TGG	0.482595	1_4:+16912397	None:intergenic
AACACGCCCACGCTTAACAG+TGG	0.676401	1_4:-16912911	Msa0153530:CDS
AACCAACACTGCCAAAGTGT+CGG	0.629352	1_4:-16910776	Msa0153530:CDS
AACCTTGATTGCAACTACAT+CGG	0.632635	1_4:+16912441	None:intergenic
AACTGCAGTACAGCTTCTAA+TGG	0.466015	1_4:-16912524	Msa0153530:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CAATGAATCCAAAATATACG+CGG	-	chr1_4:16910398-16910417	Msa0153530:CDS	30.0%
AATGAATCCAAAATATACGC+GGG	-	chr1_4:16910399-16910418	Msa0153530:CDS	30.0%
TTCTTTGCTTGAAATGGTAT+AGG	+	chr1_4:16910547-16910566	None:intergenic	30.0%
GGTTATTATAATAGTTACCG+TGG	-	chr1_4:16910600-16910619	Msa0153530:CDS	30.0%
ATTCCATTATCAATCGATCT+TGG	+	chr1_4:16910672-16910691	None:intergenic	30.0%
TGAACCCAAATAAAGTTGTT+CGG	+	chr1_4:16911219-16911238	None:intergenic	30.0%
GTTTATCACTTCATCTAGAA+AGG	-	chr1_4:16911306-16911325	Msa0153530:CDS	30.0%
TTGACAATTTCACTTAACAG+GGG	+	chr1_4:16911388-16911407	None:intergenic	30.0%
GCATAGTAAATGGTTGTTTA+CGG	+	chr1_4:16911421-16911440	None:intergenic	30.0%
ACCATTTACTATGCATGATT+TGG	-	chr1_4:16911427-16911446	Msa0153530:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	16910207	16913245	16910207	ID=Msa0153530;Name=Msa0153530
chr1_4	mRNA	16910207	16913245	16910207	ID=Msa0153530-mRNA-1;Parent=Msa0153530;Name=Msa0153530-mRNA-1;_AED=0.05;_eAED=0.05;_QI=0\|0.66\|0\|1\|1\|1\|4\|0\|705
chr1_4	exon	16912405	16913245	16912405	ID=Msa0153530-mRNA-1:exon:6484;Parent=Msa0153530-mRNA-1
chr1_4	exon	16911720	16911878	16911720	ID=Msa0153530-mRNA-1:exon:6483;Parent=Msa0153530-mRNA-1
chr1_4	exon	16910963	16911430	16910963	ID=Msa0153530-mRNA-1:exon:6482;Parent=Msa0153530-mRNA-1
chr1_4	exon	16910207	16910856	16910207	ID=Msa0153530-mRNA-1:exon:6481;Parent=Msa0153530-mRNA-1
chr1_4	CDS	16912405	16913245	16912405	ID=Msa0153530-mRNA-1:cds;Parent=Msa0153530-mRNA-1
chr1_4	CDS	16911720	16911878	16911720	ID=Msa0153530-mRNA-1:cds;Parent=Msa0153530-mRNA-1
chr1_4	CDS	16910963	16911430	16910963	ID=Msa0153530-mRNA-1:cds;Parent=Msa0153530-mRNA-1
chr1_4	CDS	16910207	16910856	16910207	ID=Msa0153530-mRNA-1:cds;Parent=Msa0153530-mRNA-1

Gene Sequence

>Msa0153530

ATGAAGGGGAAGCAAGTGTATGCGTTAATGGTTGCTCCATTAACCATGGTGGTAGTACTGGTAGGAACAGTTGCAGCAAATAAATGCAGTCACACTTGTGGAGGTAAAGAAATTCCATATCCATTTGCCATAAACGACACTTCATCAAATTCAGATTGTTTTGTGGGTGATAAATTAATTCCGCTCATTTGCAATGAATCCAAAATATACGCGGGTGATAACTTGGAAGTGTTAAACGTAAGCATTTCCAAAGCTGAAATCGACGTGCTATTTTATGTCTCCAAGTATTGCGGTGCTGATGATGATGAGAATAACACGCCCACGCTTAACAGTGGTTCCTATACCATTTCAAGCAAAGAAAACAAGTTTGTTACCGTTGGTAATAACAGTTACGGTTATTATAATAGTTACCGTGGTGAGAACACTTATTCAACAGGGTGTTTAACAAGAACTTTTGGTGATCCAAGATCGATTGATAATGGAATATGTTCGGGTATAGGGTGTTGCCAAGTTGATATTCCTCCTAGAATGTGGAATATAAGTATAGAAGCTACTAATTTCAACAATGAATCCGAATTTTGTAGCTATGCATTTGTTGTTAAGAACGATAACTATACTTTCAATTCAACTCATTTATTAAAAGGAGGTTTTCCTTTCAAGGAATTACCTGTGGTTCTTGACTGGACCATTGGTGAGGAAAACTGCAGTACAGCTTCTAATGGAGTCAACTATGGGTGCAAGAACAATAGCTATTGTGATGACAAGGACACAGAATTTGGTTACCGATGTAGTTGCAATCAAGGTTATGAAGGAAATCCTTATGAACCCAATGGCTGCACAGATATTGACGAATGTGAAAAAAACCAACATAATTGCAAAACAAAAGCATATTGTCAAAACACCGATGGCAATTACACATGTGTGTGTCCAAAGTGGTATTCCGGAGATGGAACAAAGGAAGGAGGGTGCAAAAATTCAATAGTATTACCTACTGTCCTTGGAGTAGGAGCAACACTTATTCTTCTTTTGGTGGGCACATTCTCGTACTTAATATATCATAAGAGGAAATTCATCAAAATGAAAGAGAAATTCTTTGAGCAGAATGGTGGTTTGATTTTGAAACAAAAACTCTCCACAAGAGAAGACTCTTCCTCTCAAACAAGACAAGACTCATCAACTCAAAGTGCTAAAATATTCACACAAGATCAGCTAAACAAGGCCACCAAAAACTTCGACGAGAATTTAATCATTGGCAAAGGTGGTTTTGGTACTGTTTACAAAGGATTTCTGAATGATAAAAGAATTGTTGCTATCAAAAAGTCAAAAATAATAGATAGAAGCCAAATCAATCAATTCATTAATGAGGTGGTTGTTCTCTCTCAAATAAATCATAGGAATGTTGTCAAACTCTTGGGGTGTTGTTTAGAGACAGAAGTTCCTTTGTTGGTTTATGAATTTGTTAGCAATGCTGCCTCAATACCGATTATCCATAGAGATGTCAAGAGTGATAACATTCTCTTAGATGGAACCAACACTGCCAAAGTGTCGGATTTTGGAGCTTCAAGATTGGTTCCACTTGATCAAACTGAAGTAGCCACAGTAGTGCAAGGAACTATTGGCTACTTAGATCCAGAGTATATGCAAACAAGCCAATTGACTGAGAAAAGTGATGTCTATAGCTTTGGAGTAGTTCTTGTGGAGCTGCTAACGGGGGAGAAACCTTTTAGTTTTGGTAGGCCGGAAGAGAGAAGAAGTCTTGCTATGCACTTTTTATCTTGCTTGAAAACAGAAAATGTGTTTGAAGTTATTCAAGATGGTATGTTGAATGAAGAAAATAAACAAGAGATCAAGGAGGTGGCTGTTCTTGCGGCGAAGTGTTTGAGACTTAGAGGGGAAGAAAGACCAAGCATGAAGGAAGTGGCTATGGAATTGGAGGGAATGAGGTTAATGAATAAGCACTCTTGGATCAATGATGACTTAAATGTAGAGGAGAGTATGTGCTTGCTTCATGAATCATCATCTAACATTTATGAACCTGGTGATAGCAGCAAACATGGAGATACCAGATATGACAGCTTGAAAGATCATGCACTGATAGCATTGGATGATGGAAGATGA

Protein sequence

>Msa0153530

MKGKQVYALMVAPLTMVVVLVGTVAANKCSHTCGGKEIPYPFAINDTSSNSDCFVGDKLIPLICNESKIYAGDNLEVLNVSISKAEIDVLFYVSKYCGADDDENNTPTLNSGSYTISSKENKFVTVGNNSYGYYNSYRGENTYSTGCLTRTFGDPRSIDNGICSGIGCCQVDIPPRMWNISIEATNFNNESEFCSYAFVVKNDNYTFNSTHLLKGGFPFKELPVVLDWTIGEENCSTASNGVNYGCKNNSYCDDKDTEFGYRCSCNQGYEGNPYEPNGCTDIDECEKNQHNCKTKAYCQNTDGNYTCVCPKWYSGDGTKEGGCKNSIVLPTVLGVGATLILLLVGTFSYLIYHKRKFIKMKEKFFEQNGGLILKQKLSTREDSSSQTRQDSSTQSAKIFTQDQLNKATKNFDENLIIGKGGFGTVYKGFLNDKRIVAIKKSKIIDRSQINQFINEVVVLSQINHRNVVKLLGCCLETEVPLLVYEFVSNAASIPIIHRDVKSDNILLDGTNTAKVSDFGASRLVPLDQTEVATVVQGTIGYLDPEYMQTSQLTEKSDVYSFGVVLVELLTGEKPFSFGRPEERRSLAMHFLSCLKTENVFEVIQDGMLNEENKQEIKEVAVLAAKCLRLRGEERPSMKEVAMELEGMRLMNKHSWINDDLNVEESMCLLHESSSNIYEPGDSSKHGDTRYDSLKDHALIALDDGR*