AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0154380

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0154380 MtrunA17_Chr1g0164731 92.945 567 39 1 1 566 18 584 0.0 1079
Msa0154380 MtrunA17_Chr1g0164761 71.256 581 120 5 22 575 2 562 0.0 836
Msa0154380 MtrunA17_Chr5g0410721 57.023 477 203 2 10 485 71 546 0.0 588
Msa0154380 MtrunA17_Chr3g0116441 36.842 380 202 9 21 379 11 373 7.49e-67 236
Msa0154380 MtrunA17_Chr8g0380981 35.825 388 174 10 24 345 39 417 2.09e-56 205
Msa0154380 MtrunA17_Chr1g0189211 29.981 537 284 14 25 482 147 670 2.99e-55 202
Msa0154380 MtrunA17_Chr5g0397501 33.023 430 242 13 107 511 302 710 2.36e-54 199
Msa0154380 MtrunA17_Chr7g0214951 74.167 120 13 2 201 319 99 201 7.35e-54 182
Msa0154380 MtrunA17_Chr2g0303101 29.139 604 326 16 24 542 48 634 2.78e-52 192
Msa0154380 MtrunA17_Chr1g0199511 44.681 188 100 1 172 359 291 474 1.45e-50 188
Msa0154380 MtrunA17_Chr7g0261401 25.837 418 281 9 176 568 338 751 4.56e-31 129
Msa0154380 MtrunA17_Chr7g0214941 55.556 63 26 2 474 536 14 74 6.31e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0154380 AT5G46910.1 54.639 485 218 2 2 485 77 560 0.0 573
Msa0154380 AT5G46910.2 54.639 485 218 2 2 485 77 560 0.0 571
Msa0154380 AT3G48430.1 33.947 380 209 7 17 359 9 383 7.28e-59 213
Msa0154380 AT2G34880.1 30.394 533 277 18 25 482 58 571 9.44e-58 207
Msa0154380 AT1G30810.3 29.981 537 283 13 24 482 55 576 1.48e-55 201
Msa0154380 AT1G30810.2 29.981 537 283 13 24 482 55 576 1.48e-55 201
Msa0154380 AT1G30810.1 29.981 537 283 13 24 482 55 576 1.48e-55 201
Msa0154380 AT1G63490.1 41.328 271 133 6 107 360 46 307 1.68e-55 203
Msa0154380 AT1G63490.4 40.511 274 131 6 107 360 299 560 2.08e-55 202
Msa0154380 AT1G63490.2 40.511 274 131 6 107 360 299 560 2.20e-55 202
Msa0154380 AT1G08620.2 29.908 545 282 14 25 482 143 674 9.79e-55 201
Msa0154380 AT1G08620.4 29.908 545 282 14 25 482 143 674 9.79e-55 201
Msa0154380 AT1G08620.1 29.908 545 282 14 25 482 143 674 9.79e-55 201
Msa0154380 AT1G08620.3 29.908 545 282 14 25 482 157 688 1.10e-54 201
Msa0154380 AT4G20400.1 28.358 536 300 13 24 484 52 578 2.16e-54 199
Msa0154380 AT5G04240.1 47.340 188 95 1 172 359 259 442 1.53e-51 191
Msa0154380 AT4G20400.2 28.817 524 289 13 36 484 7 521 2.80e-51 189
Msa0154380 AT2G38950.1 27.519 516 303 17 22 482 102 601 1.41e-36 145
Msa0154380 AT1G63490.3 50.575 87 43 0 274 360 15 101 1.31e-23 106

Find 94 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGATTTGGATGATCTAAAT+TGG 0.198966 1_4:-18881214 Msa0154380:CDS
GTGCTATTGTACAGTCTTTC+TGG 0.204802 1_4:-18878979 Msa0154380:CDS
TGTCTTAATGAAAGAGAAAA+AGG 0.225632 1_4:-18880900 Msa0154380:CDS
TCCTGCAATGTCTTCTGTTT+TGG 0.237851 1_4:-18878129 Msa0154380:CDS
AATCCAGCATGATATGAATT+TGG 0.254423 1_4:+18879714 None:intergenic
TTGGTGATTGGTTTCCATTT+GGG 0.283394 1_4:-18879147 Msa0154380:CDS
CAAATCGAGTAAATTTGATT+TGG 0.293057 1_4:-18881228 Msa0154380:CDS
GCTCCCGAGGCTTCTAAATA+TGG 0.295796 1_4:-18881110 Msa0154380:intron
AATGATGTATTTGAAGAGTT+TGG 0.308315 1_4:+18878899 None:intergenic
GCAGGATTAGTGGCACTAAT+TGG 0.313079 1_4:+18880929 None:intergenic
TGTATCCTTCTTATTCCATT+TGG 0.315147 1_4:+18880838 None:intergenic
AGTTCCTCATATGGAATTAT+AGG 0.316333 1_4:+18879085 None:intergenic
GAGAAGGGAACAGTTGAGTA+TGG 0.324983 1_4:-18880572 Msa0154380:CDS
ATAAAGGGTTCGCGGTCTTT+TGG 0.337629 1_4:+18878190 None:intergenic
AACTCTTCAAATACATCATT+AGG 0.338416 1_4:-18878896 Msa0154380:CDS
ATTGGTGATTGGTTTCCATT+TGG 0.339040 1_4:-18879148 Msa0154380:CDS
TTACAAAGAAGTTCCTCATA+TGG 0.344918 1_4:+18879076 None:intergenic
GGATGGTAAACTGGACATTC+TGG 0.345953 1_4:+18881179 None:intergenic
TTACCATGGAAAAGGCAAAT+TGG 0.347643 1_4:+18878800 None:intergenic
TGTTCTGATGGATGGTAAAC+TGG 0.349116 1_4:+18881170 None:intergenic
CTCGTTCCAAGTTTGTCATC+AGG 0.367879 1_4:+18880515 None:intergenic
CTTGTTACAACATGATTTGC+CGG 0.374726 1_4:-18879781 Msa0154380:CDS
TAAACTTTGCTATTGGTGAT+TGG 0.380389 1_4:-18879159 Msa0154380:CDS
CTTTGCACCGCCTTGTAAAC+CGG 0.382897 1_4:+18879762 None:intergenic
ACCGAAAGCAGAATGATTAT+CGG 0.415940 1_4:-18878373 Msa0154380:intron
TCATGTTGTAACAAGACATT+AGG 0.418260 1_4:+18879789 None:intergenic
AACGAGCAAATTGAATTTGA+AGG 0.422359 1_4:-18880498 Msa0154380:intron
ACAAGAATTTGAGCATCCTT+TGG 0.422698 1_4:-18881150 Msa0154380:CDS
GACAAAATCAGCAGGATTAG+TGG 0.424845 1_4:+18880919 None:intergenic
TCAATTTGAAAAGACTGTCT+TGG 0.425142 1_4:-18879898 Msa0154380:CDS
TTCTGAACTGCGAAATCTGT+TGG 0.437159 1_4:+18880649 None:intergenic
ACATGATTTGCCGGTTTACA+AGG 0.444156 1_4:-18879772 Msa0154380:CDS
GAAGCTGTAAACTTTGCTAT+TGG 0.453482 1_4:-18879166 Msa0154380:CDS
TTCCATTTGGAAAGCCTAAG+AGG 0.455877 1_4:+18880851 None:intergenic
TTGCTTGCTGTAGCCCCAAA+TGG 0.456412 1_4:+18879133 None:intergenic
TGCTCAAATTCTTGTTCTGA+TGG 0.458499 1_4:+18881158 None:intergenic
GCTACCTATAATTCCATATG+AGG 0.458631 1_4:-18879089 Msa0154380:CDS
CTGATCCTATGCTTTACATT+GGG 0.461409 1_4:-18880127 Msa0154380:CDS
TTCATTAAGACAAAATCAGC+AGG 0.464993 1_4:+18880911 None:intergenic
TGGTGATTGGTTTCCATTTG+GGG 0.472838 1_4:-18879146 Msa0154380:CDS
ACGATATGATGCCATGTCTT+CGG 0.473676 1_4:+18879000 None:intergenic
TACAAGGCGGTGCAAAGGCC+AGG 0.479049 1_4:-18879756 Msa0154380:CDS
ACTGATCCTATGCTTTACAT+TGG 0.488180 1_4:-18880128 Msa0154380:CDS
TTGAAGATCACTATTTGTAC+AGG 0.488775 1_4:-18880076 Msa0154380:intron
TTTCCAAATTCATATCATGC+TGG 0.496493 1_4:-18879717 Msa0154380:CDS
GGTGGAAATAAAACTTGGTA+TGG 0.496778 1_4:-18879942 Msa0154380:CDS
AGCCTCTTAGGCTTTCCAAA+TGG 0.497403 1_4:-18880853 Msa0154380:CDS
ATTTACATATCAAGAATTTG+AGG 0.498743 1_4:-18880693 Msa0154380:CDS
TGACATGCAGCCTCTGCCAT+AGG 0.500330 1_4:-18878868 Msa0154380:CDS
AATTGAGAGGCTGCAGAGCT+AGG 0.502653 1_4:+18879915 None:intergenic
GCCAAAACAGAAGACATTGC+AGG 0.509540 1_4:+18878128 None:intergenic
TTAGCAAAACATGGAGAGAA+TGG 0.514270 1_4:-18879852 Msa0154380:CDS
CAAATTCTTGTTCTGATGGA+TGG 0.515818 1_4:+18881162 None:intergenic
TGCTATTCAGTATGTTTGCA+TGG 0.518171 1_4:-18880103 Msa0154380:CDS
GCTTTCCAAATGGAATAAGA+AGG 0.522177 1_4:-18880843 Msa0154380:CDS
AAGCTACATAACAGTCCCTA+TGG 0.525692 1_4:+18878852 None:intergenic
CGAAATGATACATGGAGAGA+AGG 0.529280 1_4:-18880588 Msa0154380:CDS
TTACCTTCCTCTCTTAGAGA+AGG 0.529901 1_4:+18880811 None:intergenic
ACTCAGGTGGAAATAAAACT+TGG 0.530514 1_4:-18879947 Msa0154380:CDS
TGTTGTAACAAGACATTAGG+AGG 0.534027 1_4:+18879792 None:intergenic
CTCAGGTTATAAAGGGTTCG+CGG 0.534608 1_4:+18878182 None:intergenic
AAGGTGATGACAAACTCTCC+TGG 0.548617 1_4:+18879738 None:intergenic
GAAGAACTGTCAACTCTGCA+AGG 0.553779 1_4:-18878331 Msa0154380:CDS
GAGTATGGAGTCAACATTGA+GGG 0.553863 1_4:-18880557 Msa0154380:CDS
ATGGAAAAGGCAAATTGGAT+AGG 0.554570 1_4:+18878805 None:intergenic
TGTGATCCTGATGACAAACT+TGG 0.555148 1_4:-18880521 Msa0154380:CDS
GACATGCAGCCTCTGCCATA+GGG 0.558808 1_4:-18878867 Msa0154380:CDS
CGGTTTACAAGGCGGTGCAA+AGG 0.563828 1_4:-18879761 Msa0154380:CDS
TGCTTCTCCATCAGTGATGA+GGG 0.565971 1_4:-18878213 Msa0154380:CDS
TAACCTTCTCTAAGAGAGGA+AGG 0.566744 1_4:-18880814 Msa0154380:intron
ATACCATATTTAGAAGCCTC+GGG 0.566769 1_4:+18881107 None:intergenic
CAAGACATTAGGAGGGAACA+TGG 0.568622 1_4:+18879800 None:intergenic
CATACCATATTTAGAAGCCT+CGG 0.570675 1_4:+18881106 None:intergenic
TTCTGGCACGAAATGATACA+TGG 0.572727 1_4:-18880596 Msa0154380:CDS
CATAAGCAAAGCCGAAGACA+TGG 0.576450 1_4:-18879011 Msa0154380:CDS
GAAATGATACATGGAGAGAA+GGG 0.582021 1_4:-18880587 Msa0154380:CDS
ATTTGAGGCTATAGCAAACA+AGG 0.582945 1_4:-18880678 Msa0154380:CDS
ATGCTTCTCCATCAGTGATG+AGG 0.583640 1_4:-18878214 Msa0154380:CDS
TATCATGCTGGATTTAGTCA+TGG 0.587724 1_4:-18879705 Msa0154380:intron
AGCATAAATTATCATCACTC+AGG 0.587780 1_4:-18879963 Msa0154380:CDS
TCTACAGAAAATTGCTCCCG+AGG 0.589922 1_4:-18881123 Msa0154380:CDS
CATAACAGTCCCTATGGCAG+AGG 0.595084 1_4:+18878858 None:intergenic
ACAATAACCTTCTCTAAGAG+AGG 0.603926 1_4:-18880818 Msa0154380:CDS
ATAAATTATCATCACTCAGG+TGG 0.605184 1_4:-18879960 Msa0154380:CDS
AGCATCCCAATGTAAAGCAT+AGG 0.618659 1_4:+18880122 None:intergenic
AACAAAATCTTAGCAAAACA+TGG 0.621242 1_4:-18879861 Msa0154380:CDS
AAGAACTGTCAACTCTGCAA+GGG 0.630020 1_4:-18878330 Msa0154380:CDS
CCGTTGCGTTTAGTTGACAG+AGG 0.635990 1_4:-18880242 Msa0154380:CDS
AGCATGATATGAATTTGGAA+AGG 0.636839 1_4:+18879719 None:intergenic
TGATTTGCCGGTTTACAAGG+CGG 0.657584 1_4:-18879769 Msa0154380:CDS
TGAGTATGGAGTCAACATTG+AGG 0.665976 1_4:-18880558 Msa0154380:CDS
CCTCTGTCAACTAAACGCAA+CGG 0.668558 1_4:+18880242 None:intergenic
ACTAAACGCAACGGAGACTG+CGG 0.678157 1_4:+18880251 None:intergenic
GTTGTAACAAGACATTAGGA+GGG 0.697642 1_4:+18879793 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTCTGTTTTATAATTTT+AGG - chr1_4:18879204-18879223 Msa0154380:intron 10.0%
!!! AATTCTGTTTTATAATTTTA+GGG - chr1_4:18879205-18879224 Msa0154380:intron 10.0%
!!! TAGTATATGTTTTTTTTTTC+AGG - chr1_4:18878389-18878408 Msa0154380:intron 15.0%
!! AGGTAAAAAATAAACTAAAT+TGG - chr1_4:18878566-18878585 Msa0154380:intron 15.0%
!! AGTTTATTTATTCATCATTT+AGG + chr1_4:18878615-18878634 None:intergenic 15.0%
!!! TTTCATTTGATTTTAGAATT+TGG - chr1_4:18879078-18879097 Msa0154380:CDS 15.0%
!! TTTATTCTTGATAGATAAAT+TGG - chr1_4:18880623-18880642 Msa0154380:CDS 15.0%
!!! TTCTTGATATGTAAATTTTC+TGG + chr1_4:18878663-18878682 None:intergenic 20.0%
!! ATTTACATATCAAGAATTTG+AGG - chr1_4:18878667-18878686 Msa0154380:CDS 20.0%
!! AAAAGATGTTAATAGAGAAA+GGG + chr1_4:18878920-18878939 None:intergenic 20.0%
!! AAAAAGATGTTAATAGAGAA+AGG + chr1_4:18878921-18878940 None:intergenic 20.0%
!!! TACTGAAGTATTTTAAATAG+CGG + chr1_4:18880071-18880090 None:intergenic 20.0%
!!! ACTATTTTAATGAAGTTTTG+AGG + chr1_4:18880883-18880902 None:intergenic 20.0%
!! TTTCAAAAGTTACAATTAGT+TGG + chr1_4:18880962-18880981 None:intergenic 20.0%
! CAAATCGAGTAAATTTGATT+TGG - chr1_4:18878132-18878151 Msa0154380:CDS 25.0%
!!! TTGATTTGGATGATCTAAAT+TGG - chr1_4:18878146-18878165 Msa0154380:CDS 25.0%
!! ATTTTCTGTAGATAAACCAA+AGG + chr1_4:18878229-18878248 None:intergenic 25.0%
! TGTCTTAATGAAAGAGAAAA+AGG - chr1_4:18878460-18878479 Msa0154380:intron 25.0%
! AAAGATGTTAATAGAGAAAG+GGG + chr1_4:18878919-18878938 None:intergenic 25.0%
!! TTGAAGATTTTCAGAATCAT+TGG + chr1_4:18878972-18878991 None:intergenic 25.0%
! TGTTGTAATATTCTTCTGTA+AGG - chr1_4:18879033-18879052 Msa0154380:CDS 25.0%
! GTTGTAATATTCTTCTGTAA+GGG - chr1_4:18879034-18879053 Msa0154380:CDS 25.0%
! AACAAAATCTTAGCAAAACA+TGG - chr1_4:18879499-18879518 Msa0154380:intron 25.0%
!!! TTGTTTTTTGTGCAAGAAAT+TGG + chr1_4:18879540-18879559 None:intergenic 25.0%
! AGATCGTTCAAATAATAGAA+AGG - chr1_4:18879799-18879818 Msa0154380:CDS 25.0%
! TTCAAGGTTAATCTATAAAC+TGG - chr1_4:18879827-18879846 Msa0154380:CDS 25.0%
! TCAAGGTTAATCTATAAACT+GGG - chr1_4:18879828-18879847 Msa0154380:CDS 25.0%
! GATTACTTGTGTAAATAGTA+TGG + chr1_4:18879854-18879873 None:intergenic 25.0%
! ATTTCCATTATTCAAGCTTA+GGG + chr1_4:18879883-18879902 None:intergenic 25.0%
!! CTCTGTAATCTGTATATTTT+TGG - chr1_4:18879970-18879989 Msa0154380:intron 25.0%
!!! ACTGAAGTATTTTAAATAGC+GGG + chr1_4:18880070-18880089 None:intergenic 25.0%
! TTCATATTCTTGTTGAGTTT+AGG - chr1_4:18880158-18880177 Msa0154380:intron 25.0%
! AATGATGTATTTGAAGAGTT+TGG + chr1_4:18880464-18880483 None:intergenic 25.0%
! AACTCTTCAAATACATCATT+AGG - chr1_4:18880464-18880483 Msa0154380:intron 25.0%
! AATTCTCAAATAGACTAATG+AGG + chr1_4:18880588-18880607 None:intergenic 25.0%
! ATGTTAAAGGAGAATGTTAA+AGG + chr1_4:18880790-18880809 None:intergenic 25.0%
!! ATGTTTTCTCAGGTTATAAA+GGG + chr1_4:18881188-18881207 None:intergenic 25.0%
!!! ATTTTGATTCATGTTTTCTC+AGG + chr1_4:18881198-18881217 None:intergenic 25.0%
TTCATTAAGACAAAATCAGC+AGG + chr1_4:18878452-18878471 None:intergenic 30.0%
! AAACAATTTTACAGCCTCTT+AGG - chr1_4:18878495-18878514 Msa0154380:intron 30.0%
TGTATCCTTCTTATTCCATT+TGG + chr1_4:18878525-18878544 None:intergenic 30.0%
AACGAGCAAATTGAATTTGA+AGG - chr1_4:18878862-18878881 Msa0154380:CDS 30.0%
!!! TTTGATTTTAGAATTTGGCG+CGG - chr1_4:18879083-18879102 Msa0154380:CDS 30.0%
TTGAAGATCACTATTTGTAC+AGG - chr1_4:18879284-18879303 Msa0154380:intron 30.0%
ATGCTGCAAAAACAAAAAGA+CGG + chr1_4:18879382-18879401 None:intergenic 30.0%
AGCATAAATTATCATCACTC+AGG - chr1_4:18879397-18879416 Msa0154380:intron 30.0%
ATAAATTATCATCACTCAGG+TGG - chr1_4:18879400-18879419 Msa0154380:intron 30.0%
TCAATTTGAAAAGACTGTCT+TGG - chr1_4:18879462-18879481 Msa0154380:intron 30.0%
TCATGTTGTAACAAGACATT+AGG + chr1_4:18879574-18879593 None:intergenic 30.0%
AGCATGATATGAATTTGGAA+AGG + chr1_4:18879644-18879663 None:intergenic 30.0%
TTTCCAAATTCATATCATGC+TGG - chr1_4:18879643-18879662 Msa0154380:intron 30.0%
AATCCAGCATGATATGAATT+TGG + chr1_4:18879649-18879668 None:intergenic 30.0%
! TAATAGAAAGGTACGATTCA+AGG - chr1_4:18879811-18879830 Msa0154380:CDS 30.0%
TAAACCCTAAGCTTGAATAA+TGG - chr1_4:18879876-18879895 Msa0154380:CDS 30.0%
CATTTCCATTATTCAAGCTT+AGG + chr1_4:18879884-18879903 None:intergenic 30.0%
ATATACAGATTACAGAGAAC+AGG + chr1_4:18879967-18879986 None:intergenic 30.0%
!!! AATCTGTATATTTTTGGACC+AGG - chr1_4:18879976-18879995 Msa0154380:intron 30.0%
CAGTTTCTGCATATATATAG+AGG - chr1_4:18880012-18880031 Msa0154380:intron 30.0%
ATTTCACACTAGATAGGAAT+AGG + chr1_4:18880114-18880133 None:intergenic 30.0%
CTTTAGATTTCACACTAGAT+AGG + chr1_4:18880120-18880139 None:intergenic 30.0%
TCTAGTGTGAAATCTAAAGA+TGG - chr1_4:18880121-18880140 Msa0154380:CDS 30.0%
AATCTAAAGATGGTAGCTAA+TGG - chr1_4:18880131-18880150 Msa0154380:CDS 30.0%
!!! TTGAGTTTAGGTTTTAACTG+TGG - chr1_4:18880170-18880189 Msa0154380:intron 30.0%
!! TAAACTTTGCTATTGGTGAT+TGG - chr1_4:18880201-18880220 Msa0154380:intron 30.0%
AGTTCCTCATATGGAATTAT+AGG + chr1_4:18880278-18880297 None:intergenic 30.0%
TTACAAAGAAGTTCCTCATA+TGG + chr1_4:18880287-18880306 None:intergenic 30.0%
! TATGTAACTTCTGACTTTTG+AGG + chr1_4:18880333-18880352 None:intergenic 30.0%
! TTTTACCTGTTCAAAACTCT+TGG + chr1_4:18880749-18880768 None:intergenic 30.0%
TACGTAAAGATGAAGTAATG+TGG + chr1_4:18880921-18880940 None:intergenic 30.0%
! CATGTTTTCTCAGGTTATAA+AGG + chr1_4:18881189-18881208 None:intergenic 30.0%
CAAATTCTTGTTCTGATGGA+TGG + chr1_4:18878201-18878220 None:intergenic 35.0%
TGCTCAAATTCTTGTTCTGA+TGG + chr1_4:18878205-18878224 None:intergenic 35.0%
ACAAGAATTTGAGCATCCTT+TGG - chr1_4:18878210-18878229 Msa0154380:CDS 35.0%
ATACCATATTTAGAAGCCTC+GGG + chr1_4:18878256-18878275 None:intergenic 35.0%
CATACCATATTTAGAAGCCT+CGG + chr1_4:18878257-18878276 None:intergenic 35.0%
GCTTTCCAAATGGAATAAGA+AGG - chr1_4:18878517-18878536 Msa0154380:intron 35.0%
! ACAATAACCTTCTCTAAGAG+AGG - chr1_4:18878542-18878561 Msa0154380:intron 35.0%
! ATTTGAGGCTATAGCAAACA+AGG - chr1_4:18878682-18878701 Msa0154380:CDS 35.0%
!! TAGATATCGAAAAAGCGTTC+TGG - chr1_4:18878747-18878766 Msa0154380:intron 35.0%
GAAATGATACATGGAGAGAA+GGG - chr1_4:18878773-18878792 Msa0154380:intron 35.0%
GTAATAGCTTAATCCAGACT+AGG + chr1_4:18879172-18879191 None:intergenic 35.0%
ACTGATCCTATGCTTTACAT+TGG - chr1_4:18879232-18879251 Msa0154380:intron 35.0%
CTGATCCTATGCTTTACATT+GGG - chr1_4:18879233-18879252 Msa0154380:intron 35.0%
TGCTATTCAGTATGTTTGCA+TGG - chr1_4:18879257-18879276 Msa0154380:intron 35.0%
TGCTGCAAAAACAAAAAGAC+GGG + chr1_4:18879381-18879400 None:intergenic 35.0%
ACTCAGGTGGAAATAAAACT+TGG - chr1_4:18879413-18879432 Msa0154380:intron 35.0%
GGTGGAAATAAAACTTGGTA+TGG - chr1_4:18879418-18879437 Msa0154380:intron 35.0%
! GACAGTCTTTTCAAATTGAG+AGG + chr1_4:18879461-18879480 None:intergenic 35.0%
TTAGCAAAACATGGAGAGAA+TGG - chr1_4:18879508-18879527 Msa0154380:intron 35.0%
GTTGTAACAAGACATTAGGA+GGG + chr1_4:18879570-18879589 None:intergenic 35.0%
TGTTGTAACAAGACATTAGG+AGG + chr1_4:18879571-18879590 None:intergenic 35.0%
! CTTGTTACAACATGATTTGC+CGG - chr1_4:18879579-18879598 Msa0154380:intron 35.0%
TATCATGCTGGATTTAGTCA+TGG - chr1_4:18879655-18879674 Msa0154380:intron 35.0%
AAACTATGAGCAATAGCCAA+CGG + chr1_4:18879765-18879784 None:intergenic 35.0%
TGGCTATTGCTCATAGTTTA+CGG - chr1_4:18879766-18879785 Msa0154380:CDS 35.0%
CTCATAGTTTACGGATATGT+AGG - chr1_4:18879775-18879794 Msa0154380:CDS 35.0%
! GAAGCTGTAAACTTTGCTAT+TGG - chr1_4:18880194-18880213 Msa0154380:intron 35.0%
!! ATTGGTGATTGGTTTCCATT+TGG - chr1_4:18880212-18880231 Msa0154380:intron 35.0%
!! TTGGTGATTGGTTTCCATTT+GGG - chr1_4:18880213-18880232 Msa0154380:intron 35.0%
GCTACCTATAATTCCATATG+AGG - chr1_4:18880271-18880290 Msa0154380:intron 35.0%
ATGGAAAAGGCAAATTGGAT+AGG + chr1_4:18880558-18880577 None:intergenic 35.0%
! TATCCAATTTGCCTTTTCCA+TGG - chr1_4:18880557-18880576 Msa0154380:CDS 35.0%
TTACCATGGAAAAGGCAAAT+TGG + chr1_4:18880563-18880582 None:intergenic 35.0%
! ATGAGGTTTTACCATGGAAA+AGG + chr1_4:18880571-18880590 None:intergenic 35.0%
! AGACTAATGAGGTTTTACCA+TGG + chr1_4:18880577-18880596 None:intergenic 35.0%
! TGCCAAAAACTTGCTTATGT+GGG - chr1_4:18880674-18880693 Msa0154380:CDS 35.0%
CACGCACTCAATTATGTTAA+AGG + chr1_4:18880803-18880822 None:intergenic 35.0%
ACCGAAAGCAGAATGATTAT+CGG - chr1_4:18880987-18881006 Msa0154380:intron 35.0%
! TGTTCTGATGGATGGTAAAC+TGG + chr1_4:18878193-18878212 None:intergenic 40.0%
GACAAAATCAGCAGGATTAG+TGG + chr1_4:18878444-18878463 None:intergenic 40.0%
TTCCATTTGGAAAGCCTAAG+AGG + chr1_4:18878512-18878531 None:intergenic 40.0%
! TAACCTTCTCTAAGAGAGGA+AGG - chr1_4:18878546-18878565 Msa0154380:intron 40.0%
TTACCTTCCTCTCTTAGAGA+AGG + chr1_4:18878552-18878571 None:intergenic 40.0%
TTCTGAACTGCGAAATCTGT+TGG + chr1_4:18878714-18878733 None:intergenic 40.0%
TTCTGGCACGAAATGATACA+TGG - chr1_4:18878764-18878783 Msa0154380:intron 40.0%
CGAAATGATACATGGAGAGA+AGG - chr1_4:18878772-18878791 Msa0154380:intron 40.0%
TGAGTATGGAGTCAACATTG+AGG - chr1_4:18878802-18878821 Msa0154380:intron 40.0%
GAGTATGGAGTCAACATTGA+GGG - chr1_4:18878803-18878822 Msa0154380:intron 40.0%
TGTGATCCTGATGACAAACT+TGG - chr1_4:18878839-18878858 Msa0154380:CDS 40.0%
TTGACAGAGGAATTCCTGTA+AGG - chr1_4:18879131-18879150 Msa0154380:CDS 40.0%
!! TCCTGTAAGGAAGCCATTTT+TGG - chr1_4:18879144-18879163 Msa0154380:CDS 40.0%
TCCAAAAATGGCTTCCTTAC+AGG + chr1_4:18879148-18879167 None:intergenic 40.0%
TCCAGACTAGGTTCCAAAAA+TGG + chr1_4:18879160-18879179 None:intergenic 40.0%
AGCATCCCAATGTAAAGCAT+AGG + chr1_4:18879241-18879260 None:intergenic 40.0%
!! ACATGATTTGCCGGTTTACA+AGG - chr1_4:18879588-18879607 Msa0154380:intron 40.0%
TGTAGTCTCGGAGGAAAAAT+TGG - chr1_4:18879918-18879937 Msa0154380:CDS 40.0%
AACTGTTAGAACTTCAGTCC+TGG + chr1_4:18879997-18880016 None:intergenic 40.0%
!! TGGTGATTGGTTTCCATTTG+GGG - chr1_4:18880214-18880233 Msa0154380:intron 40.0%
ACGATATGATGCCATGTCTT+CGG + chr1_4:18880363-18880382 None:intergenic 40.0%
! GTGCTATTGTACAGTCTTTC+TGG - chr1_4:18880381-18880400 Msa0154380:intron 40.0%
AAGCTACATAACAGTCCCTA+TGG + chr1_4:18880511-18880530 None:intergenic 40.0%
! GTGCCAAAAACTTGCTTATG+TGG - chr1_4:18880673-18880692 Msa0154380:CDS 40.0%
! GCCAAAAACTTGCTTATGTG+GGG - chr1_4:18880675-18880694 Msa0154380:CDS 40.0%
! CCAAAAACTTGCTTATGTGG+GGG - chr1_4:18880676-18880695 Msa0154380:CDS 40.0%
! CAAAAACTTGCTTATGTGGG+GGG - chr1_4:18880677-18880696 Msa0154380:CDS 40.0%
GCCGATAATCATTCTGCTTT+CGG + chr1_4:18880991-18881010 None:intergenic 40.0%
AAGAACTGTCAACTCTGCAA+GGG - chr1_4:18881030-18881049 Msa0154380:intron 40.0%
! TCCTGCAATGTCTTCTGTTT+TGG - chr1_4:18881231-18881250 Msa0154380:CDS 40.0%
GGATGGTAAACTGGACATTC+TGG + chr1_4:18878184-18878203 None:intergenic 45.0%
TCTACAGAAAATTGCTCCCG+AGG - chr1_4:18878237-18878256 Msa0154380:CDS 45.0%
! TTACGCATGCGCAGAGTTTT+AGG + chr1_4:18878352-18878371 None:intergenic 45.0%
! GCAGGATTAGTGGCACTAAT+TGG + chr1_4:18878434-18878453 None:intergenic 45.0%
AGCCTCTTAGGCTTTCCAAA+TGG - chr1_4:18878507-18878526 Msa0154380:intron 45.0%
GAGAAGGGAACAGTTGAGTA+TGG - chr1_4:18878788-18878807 Msa0154380:intron 45.0%
CTCGTTCCAAGTTTGTCATC+AGG + chr1_4:18878848-18878867 None:intergenic 45.0%
CCTCTGTCAACTAAACGCAA+CGG + chr1_4:18879121-18879140 None:intergenic 45.0%
!!! GCCATTTTTGGAACCTAGTC+TGG - chr1_4:18879156-18879175 Msa0154380:CDS 45.0%
CAAGACATTAGGAGGGAACA+TGG + chr1_4:18879563-18879582 None:intergenic 45.0%
!! TGATTTGCCGGTTTACAAGG+CGG - chr1_4:18879591-18879610 Msa0154380:intron 45.0%
AAGGTGATGACAAACTCTCC+TGG + chr1_4:18879625-18879644 None:intergenic 45.0%
CTCTCTCTCTCTACATGTGT+TGG - chr1_4:18879732-18879751 Msa0154380:CDS 45.0%
! ATGTGTTGGTGTGATTCCGT+TGG - chr1_4:18879746-18879765 Msa0154380:CDS 45.0%
CTGCAGACACTATGTAGTCT+CGG - chr1_4:18879906-18879925 Msa0154380:CDS 45.0%
TACAGAGAACAGGCGAGATT+TGG + chr1_4:18879957-18879976 None:intergenic 45.0%
CATAAGCAAAGCCGAAGACA+TGG - chr1_4:18880349-18880368 Msa0154380:intron 45.0%
! CCCCCACATAAGCAAGTTTT+TGG + chr1_4:18880679-18880698 None:intergenic 45.0%
!!! TGCTGCCAAGAGTTTTGAAC+AGG - chr1_4:18880741-18880760 Msa0154380:intron 45.0%
GAAGAACTGTCAACTCTGCA+AGG - chr1_4:18881029-18881048 Msa0154380:intron 45.0%
ATGCTTCTCCATCAGTGATG+AGG - chr1_4:18881146-18881165 Msa0154380:CDS 45.0%
TGCTTCTCCATCAGTGATGA+GGG - chr1_4:18881147-18881166 Msa0154380:CDS 45.0%
! ATAAAGGGTTCGCGGTCTTT+TGG + chr1_4:18881173-18881192 None:intergenic 45.0%
CTCAGGTTATAAAGGGTTCG+CGG + chr1_4:18881181-18881200 None:intergenic 45.0%
GCCAAAACAGAAGACATTGC+AGG + chr1_4:18881235-18881254 None:intergenic 45.0%
GCTCCCGAGGCTTCTAAATA+TGG - chr1_4:18878250-18878269 Msa0154380:CDS 50.0%
ACTAAACGCAACGGAGACTG+CGG + chr1_4:18879112-18879131 None:intergenic 50.0%
CCGTTGCGTTTAGTTGACAG+AGG - chr1_4:18879118-18879137 Msa0154380:CDS 50.0%
AATTGAGAGGCTGCAGAGCT+AGG + chr1_4:18879448-18879467 None:intergenic 50.0%
CTTTGCACCGCCTTGTAAAC+CGG + chr1_4:18879601-18879620 None:intergenic 50.0%
GAGAGAGAGAGAGAGAGAGA+GGG + chr1_4:18879703-18879722 None:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+AGG + chr1_4:18879702-18879721 None:intergenic 50.0%
CAGACACTATGTAGTCTCGG+AGG - chr1_4:18879909-18879928 Msa0154380:CDS 50.0%
!! TTGCTTGCTGTAGCCCCAAA+TGG + chr1_4:18880230-18880249 None:intergenic 50.0%
CATAACAGTCCCTATGGCAG+AGG + chr1_4:18880505-18880524 None:intergenic 50.0%
! CTTTTGGCCCTCATCACTGA+TGG + chr1_4:18881157-18881176 None:intergenic 50.0%
CGGTTTACAAGGCGGTGCAA+AGG - chr1_4:18879599-18879618 Msa0154380:intron 55.0%
GAGAGAGAGAGAGAGAGGGA+GGG + chr1_4:18879699-18879718 None:intergenic 55.0%
AGAGAGAGAGAGAGAGAGGG+AGG + chr1_4:18879700-18879719 None:intergenic 55.0%
TGACATGCAGCCTCTGCCAT+AGG - chr1_4:18880492-18880511 Msa0154380:intron 55.0%
GACATGCAGCCTCTGCCATA+GGG - chr1_4:18880493-18880512 Msa0154380:intron 55.0%
TACAAGGCGGTGCAAAGGCC+AGG - chr1_4:18879604-18879623 Msa0154380:intron 60.0%
GAGAGAGAGAGAGGGAGGGA+GGG + chr1_4:18879695-18879714 None:intergenic 60.0%
AGAGAGAGAGAGAGGGAGGG+AGG + chr1_4:18879696-18879715 None:intergenic 60.0%
GAGAGAGAGGGAGGGAGGGA+GGG + chr1_4:18879691-18879710 None:intergenic 65.0%
AGAGAGAGAGGGAGGGAGGG+AGG + chr1_4:18879692-18879711 None:intergenic 65.0%
GAGAGGGAGGGAGGGAGGGA+GGG + chr1_4:18879687-18879706 None:intergenic 70.0%
AGAGAGGGAGGGAGGGAGGG+AGG + chr1_4:18879688-18879707 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 18878106 18881276 18878106 ID=Msa0154380;Name=Msa0154380
chr1_4 mRNA 18878106 18881276 18878106 ID=Msa0154380-mRNA-1;Parent=Msa0154380;Name=Msa0154380-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0|0|0|1|1|1|9|0|575
chr1_4 exon 18881111 18881276 18881111 ID=Msa0154380-mRNA-1:exon:6940;Parent=Msa0154380-mRNA-1
chr1_4 exon 18880815 18880971 18880815 ID=Msa0154380-mRNA-1:exon:6939;Parent=Msa0154380-mRNA-1
chr1_4 exon 18880499 18880718 18880499 ID=Msa0154380-mRNA-1:exon:6938;Parent=Msa0154380-mRNA-1
chr1_4 exon 18880235 18880288 18880235 ID=Msa0154380-mRNA-1:exon:6937;Parent=Msa0154380-mRNA-1
chr1_4 exon 18880077 18880156 18880077 ID=Msa0154380-mRNA-1:exon:6936;Parent=Msa0154380-mRNA-1
chr1_4 exon 18879706 18879983 18879706 ID=Msa0154380-mRNA-1:exon:6935;Parent=Msa0154380-mRNA-1
chr1_4 exon 18878804 18879202 18878804 ID=Msa0154380-mRNA-1:exon:6934;Parent=Msa0154380-mRNA-1
chr1_4 exon 18878620 18878714 18878620 ID=Msa0154380-mRNA-1:exon:6933;Parent=Msa0154380-mRNA-1
chr1_4 exon 18878106 18878384 18878106 ID=Msa0154380-mRNA-1:exon:6932;Parent=Msa0154380-mRNA-1
chr1_4 CDS 18881111 18881276 18881111 ID=Msa0154380-mRNA-1:cds;Parent=Msa0154380-mRNA-1
chr1_4 CDS 18880815 18880971 18880815 ID=Msa0154380-mRNA-1:cds;Parent=Msa0154380-mRNA-1
chr1_4 CDS 18880499 18880718 18880499 ID=Msa0154380-mRNA-1:cds;Parent=Msa0154380-mRNA-1
chr1_4 CDS 18880235 18880288 18880235 ID=Msa0154380-mRNA-1:cds;Parent=Msa0154380-mRNA-1
chr1_4 CDS 18880077 18880156 18880077 ID=Msa0154380-mRNA-1:cds;Parent=Msa0154380-mRNA-1
chr1_4 CDS 18879706 18879983 18879706 ID=Msa0154380-mRNA-1:cds;Parent=Msa0154380-mRNA-1
chr1_4 CDS 18878804 18879202 18878804 ID=Msa0154380-mRNA-1:cds;Parent=Msa0154380-mRNA-1
chr1_4 CDS 18878620 18878714 18878620 ID=Msa0154380-mRNA-1:cds;Parent=Msa0154380-mRNA-1
chr1_4 CDS 18878106 18878384 18878106 ID=Msa0154380-mRNA-1:cds;Parent=Msa0154380-mRNA-1
Gene Sequence

>Msa0154380

ATGGTTGAAAAAAGTTCAATAACTCACAAATCGAGTAAATTTGATTTGGATGATCTAAATTGGACAAACATGATTCCAGAATGTCCAGTTTACCATCCATCAGAACAAGAATTTGAGCATCCTTTGGTTTATCTACAGAAAATTGCTCCCGAGGCTTCTAAATATGGTATATGCAAAATTGTTTCTCCAATTAGTGCCACTAATCCTGCTGATTTTGTCTTAATGAAAGAGAAAAAGGACTTCAAGTTTGAAACAATTTTACAGCCTCTTAGGCTTTCCAAATGGAATAAGAAGGATACAATAACCTTCTCTAAGAGAGGAAGAAAATTTACATATCAAGAATTTGAGGCTATAGCAAACAAGGCATTTTCCAACAGATTTCGCAGTTCAGAAGAGCTTTCTTCGTTAGATATCGAAAAAGCGTTCTGGCACGAAATGATACATGGAGAGAAGGGAACAGTTGAGTATGGAGTCAACATTGAGGGTAGTGTCTTTTCATGTGATCCTGATGACAAACTTGGAACGAGCAAATTGAATTTGAAGAATTTGGCGCGGCTGCCGCAGTCTCCGTTGCGTTTAGTTGACAGAGGAATTCCTGGAATAACTGATCCTATGCTTTACATTGGGATGCTATTCAGTATGTTTGCATGGCATGTTGAAGATCACTATTTGTACAGCATAAATTATCATCACTCAGGTGGAAATAAAACTTGGTATGGTGTGCCTAGCTCTGCAGCCTCTCAATTTGAAAAGACTGTCTTGGATCATGTGTATTGCAACAAAATCTTAGCAAAACATGGAGAGAATGGTGCATTCCAATTTCTTGCACAAAAAACAACCATGTTCCCTCCTAATGTCTTGTTACAACATGATTTGCCGGTTTACAAGGCGGTGCAAAGGCCAGGAGAGTTTGTCATCACCTTTCCAAATTCATATCATGCTGGATTTAGTCATGGTTTTAACTGTGGAGAAGCTGTAAACTTTGCTATTGGTGATTGGTTTCCATTTGGGGCTACAGCAAGCAAACGTTATGCACATCTTAAAATGCTACCTATAATTCCATATGAGGAACTTCTTTGTAAAGAAGCAATGCTTATCTACAAGTCCTCAAAAGTCAGAAGTTACATAAGCAAAGCCGAAGACATGGCATCATATCGTGCTATTGTACAGTCTTTCTGGCATCTTATACAATTTTATAAGACATCACTATCGCGATTGAACAGTTCGAGAAAGTCGCCAAACTCTTCAAATACATCATTAGGTTCTGTGACATGCAGCCTCTGCCATAGGGACTGTTATGTAGCTTACTTGTTGTGCAAGAAATGCTATTCCTATCCAATTTGCCTTTTCCATGACGTCGTGCCAAAAACTTGCTTATGTGGGGGGAAATATATTGTTTTTAAGAATGACATGTCAGAACTCGAAGATGCTGCCAAGAGTTTTGAACAGAATGATTATCGGCATGAAGAAAACAGTGCTAGAAGAACTGTCAACTCTGCAAGGGCAGTATCTTCTAAAATGAAAACAAGAGTCACAGACAATGTCAAGCACAATGTTGAAGAGAATCAAAAGAAGACAAAGAATCGTCGTAGAAACAATGCTTCTCCATCAGTGATGAGGGCCAAAAGACCGCGAACCCTTTATAACCTGAGAAAACATGAATCAAAATTGATCATAGAAGCTCCTGCAATGTCTTCTGTTTTGGCGAGATCTGAAAAATCAAAATAA
Protein sequence

>Msa0154380

MVEKSSITHKSSKFDLDDLNWTNMIPECPVYHPSEQEFEHPLVYLQKIAPEASKYGICKIVSPISATNPADFVLMKEKKDFKFETILQPLRLSKWNKKDTITFSKRGRKFTYQEFEAIANKAFSNRFRSSEELSSLDIEKAFWHEMIHGEKGTVEYGVNIEGSVFSCDPDDKLGTSKLNLKNLARLPQSPLRLVDRGIPGITDPMLYIGMLFSMFAWHVEDHYLYSINYHHSGGNKTWYGVPSSAASQFEKTVLDHVYCNKILAKHGENGAFQFLAQKTTMFPPNVLLQHDLPVYKAVQRPGEFVITFPNSYHAGFSHGFNCGEAVNFAIGDWFPFGATASKRYAHLKMLPIIPYEELLCKEAMLIYKSSKVRSYISKAEDMASYRAIVQSFWHLIQFYKTSLSRLNSSRKSPNSSNTSLGSVTCSLCHRDCYVAYLLCKKCYSYPICLFHDVVPKTCLCGGKYIVFKNDMSELEDAAKSFEQNDYRHEENSARRTVNSARAVSSKMKTRVTDNVKHNVEENQKKTKNRRRNNASPSVMRAKRPRTLYNLRKHESKLIIEAPAMSSVLARSEKSK*