Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0156800 | G7I6L4 | 85.821 | 134 | 19 | 0 | 1 | 134 | 1 | 134 | 1.18e-72 | 222 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0156800 | TF | C2C2-GATA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0156800 | MtrunA17_Chr5g0399781 | 50.000 | 54 | 26 | 1 | 3 | 56 | 12 | 64 | 7.14e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAAAAGCTTCAAAGTCTA+TGG | 0.184389 | 1_4:+23489227 | Msa0156800:CDS |
| CGCTTGTGGGCTACATTTCA+AGG | 0.378248 | 1_4:+23488630 | Msa0156800:CDS |
| CAATACTATGTAACGCTTGT+GGG | 0.416623 | 1_4:+23488617 | Msa0156800:CDS |
| CCACAAGCGTTACATAGTAT+TGG | 0.447482 | 1_4:-23488616 | None:intergenic |
| TGGAGATTGGTTTGAAAGCT+CGG | 0.447768 | 1_4:-23488743 | None:intergenic |
| TGTATACCAAGCAATGAGAT+AGG | 0.462534 | 1_4:+23489316 | Msa0156800:CDS |
| ATCAGACCTTGACGTTGAAA+AGG | 0.465983 | 1_4:+23488705 | Msa0156800:CDS |
| GAAACTAAACTTTGGCGTAA+AGG | 0.477501 | 1_4:+23488580 | Msa0156800:CDS |
| TCTTCGGAAGATAGTTATCA+AGG | 0.478433 | 1_4:-23488663 | None:intergenic |
| CATAGTATTGGCTTCTCATA+CGG | 0.490978 | 1_4:-23488604 | None:intergenic |
| ATCGATTGTGAAGAATCAAA+TGG | 0.491491 | 1_4:+23489195 | Msa0156800:CDS |
| GGGCTACATTTCAAGGCTAA+AGG | 0.492067 | 1_4:+23488637 | Msa0156800:CDS |
| GGAGATTGGTTTGAAAGCTC+GGG | 0.493147 | 1_4:-23488742 | None:intergenic |
| CCAAGCAATGAGATAGGCAT+TGG | 0.496933 | 1_4:+23489322 | Msa0156800:CDS |
| ATTTGATTCTTCACAATCGA+TGG | 0.523804 | 1_4:-23489193 | None:intergenic |
| CCAATACTATGTAACGCTTG+TGG | 0.549603 | 1_4:+23488616 | Msa0156800:CDS |
| ACAAAAGCTTCAAAGTCTAT+GGG | 0.556384 | 1_4:+23489228 | Msa0156800:CDS |
| CCAATGCCTATCTCATTGCT+TGG | 0.566560 | 1_4:-23489322 | None:intergenic |
| GAGCTTTCAAACCAATCTCC+AGG | 0.568278 | 1_4:+23488745 | Msa0156800:CDS |
| CTTCGGAAGATAGTTATCAA+GGG | 0.629882 | 1_4:-23488662 | None:intergenic |
| GATTGTGAAGAATCAAATGG+AGG | 0.680011 | 1_4:+23489198 | Msa0156800:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTAATTTTTTTTGTAGAA+AGG | + | chr1_4:23489167-23489186 | Msa0156800:intron | 10.0% |
| !!! | TTTTAATTTTTTTTGTAGAA+AGG | + | chr1_4:23489167-23489186 | Msa0156800:intron | 10.0% |
| !!! | TTTATATTTGTTGAAAAATG+GGG | - | chr1_4:23488805-23488824 | None:intergenic | 15.0% |
| !!! | CTTTATATTTGTTGAAAAAT+GGG | - | chr1_4:23488806-23488825 | None:intergenic | 15.0% |
| !!! | TTTATATTTGTTGAAAAATG+GGG | - | chr1_4:23488805-23488824 | None:intergenic | 15.0% |
| !!! | CTTTATATTTGTTGAAAAAT+GGG | - | chr1_4:23488806-23488825 | None:intergenic | 15.0% |
| !!! | TTATTGCATTTTTTATTACC+TGG | - | chr1_4:23488766-23488785 | None:intergenic | 20.0% |
| !!! | GCTTTATATTTGTTGAAAAA+TGG | - | chr1_4:23488807-23488826 | None:intergenic | 20.0% |
| !!! | ATTGTAAGAATTTTCTTAAG+AGG | + | chr1_4:23488860-23488879 | Msa0156800:intron | 20.0% |
| !! | AACACTACTATTTATATATG+AGG | + | chr1_4:23488911-23488930 | Msa0156800:intron | 20.0% |
| !!! | TTTTGAATGAATTGTATTGA+TGG | + | chr1_4:23489004-23489023 | Msa0156800:intron | 20.0% |
| !!! | TTTGAATGAATTGTATTGAT+GGG | + | chr1_4:23489005-23489024 | Msa0156800:intron | 20.0% |
| !! | TTATATGTATACATCATACA+CGG | - | chr1_4:23489145-23489164 | None:intergenic | 20.0% |
| !!! | TTATTGCATTTTTTATTACC+TGG | - | chr1_4:23488766-23488785 | None:intergenic | 20.0% |
| !!! | GCTTTATATTTGTTGAAAAA+TGG | - | chr1_4:23488807-23488826 | None:intergenic | 20.0% |
| !!! | ATTGTAAGAATTTTCTTAAG+AGG | + | chr1_4:23488860-23488879 | Msa0156800:intron | 20.0% |
| !! | AACACTACTATTTATATATG+AGG | + | chr1_4:23488911-23488930 | Msa0156800:intron | 20.0% |
| !!! | TTTTGAATGAATTGTATTGA+TGG | + | chr1_4:23489004-23489023 | Msa0156800:intron | 20.0% |
| !!! | TTTGAATGAATTGTATTGAT+GGG | + | chr1_4:23489005-23489024 | Msa0156800:intron | 20.0% |
| !! | TTATATGTATACATCATACA+CGG | - | chr1_4:23489145-23489164 | None:intergenic | 20.0% |
| !!! | ATTTTTTATTACCTGGAGAT+TGG | - | chr1_4:23488759-23488778 | None:intergenic | 25.0% |
| !! | TTTCTTAAGAGGTACAATTT+AGG | + | chr1_4:23488871-23488890 | Msa0156800:intron | 25.0% |
| ! | ATTGACAATGAAAGGAATTA+GGG | - | chr1_4:23488951-23488970 | None:intergenic | 25.0% |
| ! | TATTGACAATGAAAGGAATT+AGG | - | chr1_4:23488952-23488971 | None:intergenic | 25.0% |
| !! | TTGAATGAATTGTATTGATG+GGG | + | chr1_4:23489006-23489025 | Msa0156800:intron | 25.0% |
| ! | TATCCATAAATGTATGAATG+TGG | - | chr1_4:23489054-23489073 | None:intergenic | 25.0% |
| !!! | ATTTTTTATTACCTGGAGAT+TGG | - | chr1_4:23488759-23488778 | None:intergenic | 25.0% |
| !! | TTTCTTAAGAGGTACAATTT+AGG | + | chr1_4:23488871-23488890 | Msa0156800:intron | 25.0% |
| ! | ATTGACAATGAAAGGAATTA+GGG | - | chr1_4:23488951-23488970 | None:intergenic | 25.0% |
| ! | TATTGACAATGAAAGGAATT+AGG | - | chr1_4:23488952-23488971 | None:intergenic | 25.0% |
| !! | TTGAATGAATTGTATTGATG+GGG | + | chr1_4:23489006-23489025 | Msa0156800:intron | 25.0% |
| ! | TATCCATAAATGTATGAATG+TGG | - | chr1_4:23489054-23489073 | None:intergenic | 25.0% |
| ! | TTTTGGCAGAAACTAAACTT+TGG | + | chr1_4:23488572-23488591 | Msa0156800:CDS | 30.0% |
| ATGCTCTTATTGACAATGAA+AGG | - | chr1_4:23488959-23488978 | None:intergenic | 30.0% | |
| ACTCCACATTCATACATTTA+TGG | + | chr1_4:23489048-23489067 | Msa0156800:intron | 30.0% | |
| ATTTGATTCTTCACAATCGA+TGG | - | chr1_4:23489196-23489215 | None:intergenic | 30.0% | |
| ATCGATTGTGAAGAATCAAA+TGG | + | chr1_4:23489195-23489214 | Msa0156800:CDS | 30.0% | |
| AACAAAAGCTTCAAAGTCTA+TGG | + | chr1_4:23489227-23489246 | Msa0156800:CDS | 30.0% | |
| ACAAAAGCTTCAAAGTCTAT+GGG | + | chr1_4:23489228-23489247 | Msa0156800:CDS | 30.0% | |
| ! | TTTTGGCAGAAACTAAACTT+TGG | + | chr1_4:23488572-23488591 | Msa0156800:CDS | 30.0% |
| ATGCTCTTATTGACAATGAA+AGG | - | chr1_4:23488959-23488978 | None:intergenic | 30.0% | |
| ACTCCACATTCATACATTTA+TGG | + | chr1_4:23489048-23489067 | Msa0156800:intron | 30.0% | |
| ATTTGATTCTTCACAATCGA+TGG | - | chr1_4:23489196-23489215 | None:intergenic | 30.0% | |
| ATCGATTGTGAAGAATCAAA+TGG | + | chr1_4:23489195-23489214 | Msa0156800:CDS | 30.0% | |
| AACAAAAGCTTCAAAGTCTA+TGG | + | chr1_4:23489227-23489246 | Msa0156800:CDS | 30.0% | |
| ACAAAAGCTTCAAAGTCTAT+GGG | + | chr1_4:23489228-23489247 | Msa0156800:CDS | 30.0% | |
| GAAACTAAACTTTGGCGTAA+AGG | + | chr1_4:23488580-23488599 | Msa0156800:CDS | 35.0% | |
| !! | CATAGTATTGGCTTCTCATA+CGG | - | chr1_4:23488607-23488626 | None:intergenic | 35.0% |
| CAATACTATGTAACGCTTGT+GGG | + | chr1_4:23488617-23488636 | Msa0156800:CDS | 35.0% | |
| CTTCGGAAGATAGTTATCAA+GGG | - | chr1_4:23488665-23488684 | None:intergenic | 35.0% | |
| TCTTCGGAAGATAGTTATCA+AGG | - | chr1_4:23488666-23488685 | None:intergenic | 35.0% | |
| !! | GTCTGATAACTGATTTTGCT+GGG | - | chr1_4:23488692-23488711 | None:intergenic | 35.0% |
| AGCATTCATTTGAGTAGACA+CGG | + | chr1_4:23488974-23488993 | Msa0156800:intron | 35.0% | |
| ATTTGAGTAGACACGGTATT+AGG | + | chr1_4:23488981-23489000 | Msa0156800:intron | 35.0% | |
| ! | GAATTGTATTGATGGGGATT+AGG | + | chr1_4:23489012-23489031 | Msa0156800:intron | 35.0% |
| TCATACACGGTACATATCAA+TGG | - | chr1_4:23489132-23489151 | None:intergenic | 35.0% | |
| GATTGTGAAGAATCAAATGG+AGG | + | chr1_4:23489198-23489217 | Msa0156800:CDS | 35.0% | |
| TGTATACCAAGCAATGAGAT+AGG | + | chr1_4:23489316-23489335 | Msa0156800:CDS | 35.0% | |
| GAAACTAAACTTTGGCGTAA+AGG | + | chr1_4:23488580-23488599 | Msa0156800:CDS | 35.0% | |
| !! | CATAGTATTGGCTTCTCATA+CGG | - | chr1_4:23488607-23488626 | None:intergenic | 35.0% |
| CAATACTATGTAACGCTTGT+GGG | + | chr1_4:23488617-23488636 | Msa0156800:CDS | 35.0% | |
| CTTCGGAAGATAGTTATCAA+GGG | - | chr1_4:23488665-23488684 | None:intergenic | 35.0% | |
| TCTTCGGAAGATAGTTATCA+AGG | - | chr1_4:23488666-23488685 | None:intergenic | 35.0% | |
| !! | GTCTGATAACTGATTTTGCT+GGG | - | chr1_4:23488692-23488711 | None:intergenic | 35.0% |
| AGCATTCATTTGAGTAGACA+CGG | + | chr1_4:23488974-23488993 | Msa0156800:intron | 35.0% | |
| ATTTGAGTAGACACGGTATT+AGG | + | chr1_4:23488981-23489000 | Msa0156800:intron | 35.0% | |
| ! | GAATTGTATTGATGGGGATT+AGG | + | chr1_4:23489012-23489031 | Msa0156800:intron | 35.0% |
| TCATACACGGTACATATCAA+TGG | - | chr1_4:23489132-23489151 | None:intergenic | 35.0% | |
| GATTGTGAAGAATCAAATGG+AGG | + | chr1_4:23489198-23489217 | Msa0156800:CDS | 35.0% | |
| TGTATACCAAGCAATGAGAT+AGG | + | chr1_4:23489316-23489335 | Msa0156800:CDS | 35.0% | |
| CCACAAGCGTTACATAGTAT+TGG | - | chr1_4:23488619-23488638 | None:intergenic | 40.0% | |
| CCAATACTATGTAACGCTTG+TGG | + | chr1_4:23488616-23488635 | Msa0156800:CDS | 40.0% | |
| ! | TGATTTTGCTGGGATGTCTT+CGG | - | chr1_4:23488682-23488701 | None:intergenic | 40.0% |
| !! | GGTCTGATAACTGATTTTGC+TGG | - | chr1_4:23488693-23488712 | None:intergenic | 40.0% |
| ATCAGACCTTGACGTTGAAA+AGG | + | chr1_4:23488705-23488724 | Msa0156800:CDS | 40.0% | |
| ! | CAGATTCCTTTTCAACGTCA+AGG | - | chr1_4:23488714-23488733 | None:intergenic | 40.0% |
| !! | TGGAGATTGGTTTGAAAGCT+CGG | - | chr1_4:23488746-23488765 | None:intergenic | 40.0% |
| CCACAAGCGTTACATAGTAT+TGG | - | chr1_4:23488619-23488638 | None:intergenic | 40.0% | |
| CCAATACTATGTAACGCTTG+TGG | + | chr1_4:23488616-23488635 | Msa0156800:CDS | 40.0% | |
| ! | TGATTTTGCTGGGATGTCTT+CGG | - | chr1_4:23488682-23488701 | None:intergenic | 40.0% |
| !! | GGTCTGATAACTGATTTTGC+TGG | - | chr1_4:23488693-23488712 | None:intergenic | 40.0% |
| ATCAGACCTTGACGTTGAAA+AGG | + | chr1_4:23488705-23488724 | Msa0156800:CDS | 40.0% | |
| ! | CAGATTCCTTTTCAACGTCA+AGG | - | chr1_4:23488714-23488733 | None:intergenic | 40.0% |
| !! | TGGAGATTGGTTTGAAAGCT+CGG | - | chr1_4:23488746-23488765 | None:intergenic | 40.0% |
| GGGCTACATTTCAAGGCTAA+AGG | + | chr1_4:23488637-23488656 | Msa0156800:CDS | 45.0% | |
| !! | GGAGATTGGTTTGAAAGCTC+GGG | - | chr1_4:23488745-23488764 | None:intergenic | 45.0% |
| GAGCTTTCAAACCAATCTCC+AGG | + | chr1_4:23488745-23488764 | Msa0156800:CDS | 45.0% | |
| CCAATGCCTATCTCATTGCT+TGG | - | chr1_4:23489325-23489344 | None:intergenic | 45.0% | |
| CCAAGCAATGAGATAGGCAT+TGG | + | chr1_4:23489322-23489341 | Msa0156800:CDS | 45.0% | |
| GGGCTACATTTCAAGGCTAA+AGG | + | chr1_4:23488637-23488656 | Msa0156800:CDS | 45.0% | |
| !! | GGAGATTGGTTTGAAAGCTC+GGG | - | chr1_4:23488745-23488764 | None:intergenic | 45.0% |
| GAGCTTTCAAACCAATCTCC+AGG | + | chr1_4:23488745-23488764 | Msa0156800:CDS | 45.0% | |
| CCAATGCCTATCTCATTGCT+TGG | - | chr1_4:23489325-23489344 | None:intergenic | 45.0% | |
| CCAAGCAATGAGATAGGCAT+TGG | + | chr1_4:23489322-23489341 | Msa0156800:CDS | 45.0% | |
| ! | CGCTTGTGGGCTACATTTCA+AGG | + | chr1_4:23488630-23488649 | Msa0156800:CDS | 50.0% |
| ! | CGCTTGTGGGCTACATTTCA+AGG | + | chr1_4:23488630-23488649 | Msa0156800:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_4 | gene | 23488568 | 23489390 | 23488568 | ID=Msa0156800;Name=Msa0156800 |
| chr1_4 | mRNA | 23488568 | 23489390 | 23488568 | ID=Msa0156800-mRNA-1;Parent=Msa0156800;Name=Msa0156800-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0|1|1|1|2|0|134 |
| chr1_4 | exon | 23488568 | 23488766 | 23488568 | ID=Msa0156800-mRNA-1:exon:8207;Parent=Msa0156800-mRNA-1 |
| chr1_4 | exon | 23489185 | 23489390 | 23489185 | ID=Msa0156800-mRNA-1:exon:8208;Parent=Msa0156800-mRNA-1 |
| chr1_4 | CDS | 23488568 | 23488766 | 23488568 | ID=Msa0156800-mRNA-1:cds;Parent=Msa0156800-mRNA-1 |
| chr1_4 | CDS | 23489185 | 23489390 | 23489185 | ID=Msa0156800-mRNA-1:cds;Parent=Msa0156800-mRNA-1 |
| Gene Sequence |
| Protein sequence |
