Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0156820 | G7I6L4 | 86.567 | 134 | 18 | 0 | 1 | 134 | 1 | 134 | 1.54e-73 | 224 |
| Gene ID | Type | Classification |
|---|---|---|
| Msa0156820 | TF | C2C2-GATA |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0156820 | MtrunA17_Chr5g0399781 | 50.000 | 54 | 26 | 1 | 3 | 56 | 12 | 64 | 6.93e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAAAAGCTTCAAAGTCTA+TGG | 0.184389 | 1_4:+23524182 | Msa0156820:CDS |
| CGCTTGTGGGCTACATTTCA+AGG | 0.378248 | 1_4:+23523587 | Msa0156820:CDS |
| CAATACTATGTAACGCTTGT+GGG | 0.416623 | 1_4:+23523574 | Msa0156820:CDS |
| CCACAAGCGTTACATAGTAT+TGG | 0.447482 | 1_4:-23523573 | None:intergenic |
| TGGAGATTGGTTTGAAAGCT+CGG | 0.447768 | 1_4:-23523700 | None:intergenic |
| ATCAGACCTTGACGTTGAAA+AGG | 0.465983 | 1_4:+23523662 | Msa0156820:CDS |
| GAAACTAAACTTTGGCGTAA+AGG | 0.477501 | 1_4:+23523537 | Msa0156820:CDS |
| TCTTCGGAAGATAGTTATCA+AGG | 0.478433 | 1_4:-23523620 | None:intergenic |
| TGTATACCAAGCAATGAGAT+AGG | 0.478624 | 1_4:+23524271 | Msa0156820:CDS |
| CATAGTATTGGCTTCTCATA+CGG | 0.490978 | 1_4:-23523561 | None:intergenic |
| ATCGATTGTGAAGAATCAAA+TGG | 0.491491 | 1_4:+23524150 | Msa0156820:CDS |
| GGGCTACATTTCAAGGCTAA+AGG | 0.492067 | 1_4:+23523594 | Msa0156820:CDS |
| GGAGATTGGTTTGAAAGCTC+GGG | 0.493147 | 1_4:-23523699 | None:intergenic |
| CCAAGCAATGAGATAGGCAT+TGG | 0.496933 | 1_4:+23524277 | Msa0156820:CDS |
| ATTTGATTCTTCACAATCGA+TGG | 0.523804 | 1_4:-23524148 | None:intergenic |
| CCAATACTATGTAACGCTTG+TGG | 0.549603 | 1_4:+23523573 | Msa0156820:CDS |
| ACAAAAGCTTCAAAGTCTAT+GGG | 0.556384 | 1_4:+23524183 | Msa0156820:CDS |
| CCAATGCCTATCTCATTGCT+TGG | 0.566560 | 1_4:-23524277 | None:intergenic |
| GAGCTTTCAAACCAATCTCC+AGG | 0.568278 | 1_4:+23523702 | Msa0156820:CDS |
| CTTCGGAAGATAGTTATCAA+GGG | 0.629882 | 1_4:-23523619 | None:intergenic |
| GATTGTGAAGAATCAAATGG+AGG | 0.680011 | 1_4:+23524153 | Msa0156820:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATATTTGTTGAAAAATG+GGG | - | chr1_4:23523762-23523781 | None:intergenic | 15.0% |
| !!! | CTTTATATTTGTTGAAAAAT+GGG | - | chr1_4:23523763-23523782 | None:intergenic | 15.0% |
| !!! | ACTTTTAATTTTTTGTAGAA+AGG | + | chr1_4:23524122-23524141 | Msa0156820:intron | 15.0% |
| !!! | TTTATATTTGTTGAAAAATG+GGG | - | chr1_4:23523762-23523781 | None:intergenic | 15.0% |
| !!! | CTTTATATTTGTTGAAAAAT+GGG | - | chr1_4:23523763-23523782 | None:intergenic | 15.0% |
| !!! | ACTTTTAATTTTTTGTAGAA+AGG | + | chr1_4:23524122-23524141 | Msa0156820:intron | 15.0% |
| !!! | TTATTGCATTTTTTATTACC+TGG | - | chr1_4:23523723-23523742 | None:intergenic | 20.0% |
| !!! | GCTTTATATTTGTTGAAAAA+TGG | - | chr1_4:23523764-23523783 | None:intergenic | 20.0% |
| !!! | ATTGTAAGAATTTTCTTAAG+AGG | + | chr1_4:23523817-23523836 | Msa0156820:intron | 20.0% |
| !! | AACACTACTATTTATATATG+AGG | + | chr1_4:23523868-23523887 | Msa0156820:intron | 20.0% |
| !!! | TTTTGAATGAATTGTATTGA+TGG | + | chr1_4:23523961-23523980 | Msa0156820:intron | 20.0% |
| !!! | TTTGAATGAATTGTATTGAT+GGG | + | chr1_4:23523962-23523981 | Msa0156820:intron | 20.0% |
| !! | TTATATGTATACATCATACA+CGG | - | chr1_4:23524102-23524121 | None:intergenic | 20.0% |
| !!! | TTATTGCATTTTTTATTACC+TGG | - | chr1_4:23523723-23523742 | None:intergenic | 20.0% |
| !!! | GCTTTATATTTGTTGAAAAA+TGG | - | chr1_4:23523764-23523783 | None:intergenic | 20.0% |
| !!! | ATTGTAAGAATTTTCTTAAG+AGG | + | chr1_4:23523817-23523836 | Msa0156820:intron | 20.0% |
| !! | AACACTACTATTTATATATG+AGG | + | chr1_4:23523868-23523887 | Msa0156820:intron | 20.0% |
| !!! | TTTTGAATGAATTGTATTGA+TGG | + | chr1_4:23523961-23523980 | Msa0156820:intron | 20.0% |
| !!! | TTTGAATGAATTGTATTGAT+GGG | + | chr1_4:23523962-23523981 | Msa0156820:intron | 20.0% |
| !! | TTATATGTATACATCATACA+CGG | - | chr1_4:23524102-23524121 | None:intergenic | 20.0% |
| !!! | ATTTTTTATTACCTGGAGAT+TGG | - | chr1_4:23523716-23523735 | None:intergenic | 25.0% |
| !! | TTTCTTAAGAGGTACAATTT+AGG | + | chr1_4:23523828-23523847 | Msa0156820:intron | 25.0% |
| ! | ATTGACAATGAAAGGAATTA+GGG | - | chr1_4:23523908-23523927 | None:intergenic | 25.0% |
| ! | TATTGACAATGAAAGGAATT+AGG | - | chr1_4:23523909-23523928 | None:intergenic | 25.0% |
| !! | TTGAATGAATTGTATTGATG+GGG | + | chr1_4:23523963-23523982 | Msa0156820:intron | 25.0% |
| ! | TATCCATAAATGTATGAATG+TGG | - | chr1_4:23524011-23524030 | None:intergenic | 25.0% |
| !!! | ATTTTTTATTACCTGGAGAT+TGG | - | chr1_4:23523716-23523735 | None:intergenic | 25.0% |
| !! | TTTCTTAAGAGGTACAATTT+AGG | + | chr1_4:23523828-23523847 | Msa0156820:intron | 25.0% |
| ! | ATTGACAATGAAAGGAATTA+GGG | - | chr1_4:23523908-23523927 | None:intergenic | 25.0% |
| ! | TATTGACAATGAAAGGAATT+AGG | - | chr1_4:23523909-23523928 | None:intergenic | 25.0% |
| !! | TTGAATGAATTGTATTGATG+GGG | + | chr1_4:23523963-23523982 | Msa0156820:intron | 25.0% |
| ! | TATCCATAAATGTATGAATG+TGG | - | chr1_4:23524011-23524030 | None:intergenic | 25.0% |
| ! | TTTTGGCAGAAACTAAACTT+TGG | + | chr1_4:23523529-23523548 | Msa0156820:CDS | 30.0% |
| ATGCTCTTATTGACAATGAA+AGG | - | chr1_4:23523916-23523935 | None:intergenic | 30.0% | |
| ACTCCACATTCATACATTTA+TGG | + | chr1_4:23524005-23524024 | Msa0156820:intron | 30.0% | |
| ATTTGATTCTTCACAATCGA+TGG | - | chr1_4:23524151-23524170 | None:intergenic | 30.0% | |
| ATCGATTGTGAAGAATCAAA+TGG | + | chr1_4:23524150-23524169 | Msa0156820:CDS | 30.0% | |
| AACAAAAGCTTCAAAGTCTA+TGG | + | chr1_4:23524182-23524201 | Msa0156820:CDS | 30.0% | |
| ACAAAAGCTTCAAAGTCTAT+GGG | + | chr1_4:23524183-23524202 | Msa0156820:CDS | 30.0% | |
| ! | TTTTGGCAGAAACTAAACTT+TGG | + | chr1_4:23523529-23523548 | Msa0156820:CDS | 30.0% |
| ATGCTCTTATTGACAATGAA+AGG | - | chr1_4:23523916-23523935 | None:intergenic | 30.0% | |
| ACTCCACATTCATACATTTA+TGG | + | chr1_4:23524005-23524024 | Msa0156820:intron | 30.0% | |
| ATTTGATTCTTCACAATCGA+TGG | - | chr1_4:23524151-23524170 | None:intergenic | 30.0% | |
| ATCGATTGTGAAGAATCAAA+TGG | + | chr1_4:23524150-23524169 | Msa0156820:CDS | 30.0% | |
| AACAAAAGCTTCAAAGTCTA+TGG | + | chr1_4:23524182-23524201 | Msa0156820:CDS | 30.0% | |
| ACAAAAGCTTCAAAGTCTAT+GGG | + | chr1_4:23524183-23524202 | Msa0156820:CDS | 30.0% | |
| GAAACTAAACTTTGGCGTAA+AGG | + | chr1_4:23523537-23523556 | Msa0156820:CDS | 35.0% | |
| !! | CATAGTATTGGCTTCTCATA+CGG | - | chr1_4:23523564-23523583 | None:intergenic | 35.0% |
| CAATACTATGTAACGCTTGT+GGG | + | chr1_4:23523574-23523593 | Msa0156820:CDS | 35.0% | |
| CTTCGGAAGATAGTTATCAA+GGG | - | chr1_4:23523622-23523641 | None:intergenic | 35.0% | |
| TCTTCGGAAGATAGTTATCA+AGG | - | chr1_4:23523623-23523642 | None:intergenic | 35.0% | |
| !! | GTCTGATAACTGATTTTGCT+GGG | - | chr1_4:23523649-23523668 | None:intergenic | 35.0% |
| AGCATTCATTTGAGTAGACA+CGG | + | chr1_4:23523931-23523950 | Msa0156820:intron | 35.0% | |
| ATTTGAGTAGACACGGTATT+AGG | + | chr1_4:23523938-23523957 | Msa0156820:intron | 35.0% | |
| ! | GAATTGTATTGATGGGGATT+AGG | + | chr1_4:23523969-23523988 | Msa0156820:intron | 35.0% |
| TCATACACGGTACATATCAA+TGG | - | chr1_4:23524089-23524108 | None:intergenic | 35.0% | |
| GATTGTGAAGAATCAAATGG+AGG | + | chr1_4:23524153-23524172 | Msa0156820:CDS | 35.0% | |
| TGTATACCAAGCAATGAGAT+AGG | + | chr1_4:23524271-23524290 | Msa0156820:CDS | 35.0% | |
| GAAACTAAACTTTGGCGTAA+AGG | + | chr1_4:23523537-23523556 | Msa0156820:CDS | 35.0% | |
| !! | CATAGTATTGGCTTCTCATA+CGG | - | chr1_4:23523564-23523583 | None:intergenic | 35.0% |
| CAATACTATGTAACGCTTGT+GGG | + | chr1_4:23523574-23523593 | Msa0156820:CDS | 35.0% | |
| CTTCGGAAGATAGTTATCAA+GGG | - | chr1_4:23523622-23523641 | None:intergenic | 35.0% | |
| TCTTCGGAAGATAGTTATCA+AGG | - | chr1_4:23523623-23523642 | None:intergenic | 35.0% | |
| !! | GTCTGATAACTGATTTTGCT+GGG | - | chr1_4:23523649-23523668 | None:intergenic | 35.0% |
| AGCATTCATTTGAGTAGACA+CGG | + | chr1_4:23523931-23523950 | Msa0156820:intron | 35.0% | |
| ATTTGAGTAGACACGGTATT+AGG | + | chr1_4:23523938-23523957 | Msa0156820:intron | 35.0% | |
| ! | GAATTGTATTGATGGGGATT+AGG | + | chr1_4:23523969-23523988 | Msa0156820:intron | 35.0% |
| TCATACACGGTACATATCAA+TGG | - | chr1_4:23524089-23524108 | None:intergenic | 35.0% | |
| GATTGTGAAGAATCAAATGG+AGG | + | chr1_4:23524153-23524172 | Msa0156820:CDS | 35.0% | |
| TGTATACCAAGCAATGAGAT+AGG | + | chr1_4:23524271-23524290 | Msa0156820:CDS | 35.0% | |
| CCACAAGCGTTACATAGTAT+TGG | - | chr1_4:23523576-23523595 | None:intergenic | 40.0% | |
| CCAATACTATGTAACGCTTG+TGG | + | chr1_4:23523573-23523592 | Msa0156820:CDS | 40.0% | |
| ! | TGATTTTGCTGGGATGTCTT+CGG | - | chr1_4:23523639-23523658 | None:intergenic | 40.0% |
| !! | GGTCTGATAACTGATTTTGC+TGG | - | chr1_4:23523650-23523669 | None:intergenic | 40.0% |
| ATCAGACCTTGACGTTGAAA+AGG | + | chr1_4:23523662-23523681 | Msa0156820:CDS | 40.0% | |
| ! | CAGATTCCTTTTCAACGTCA+AGG | - | chr1_4:23523671-23523690 | None:intergenic | 40.0% |
| !! | TGGAGATTGGTTTGAAAGCT+CGG | - | chr1_4:23523703-23523722 | None:intergenic | 40.0% |
| CCACAAGCGTTACATAGTAT+TGG | - | chr1_4:23523576-23523595 | None:intergenic | 40.0% | |
| CCAATACTATGTAACGCTTG+TGG | + | chr1_4:23523573-23523592 | Msa0156820:CDS | 40.0% | |
| ! | TGATTTTGCTGGGATGTCTT+CGG | - | chr1_4:23523639-23523658 | None:intergenic | 40.0% |
| !! | GGTCTGATAACTGATTTTGC+TGG | - | chr1_4:23523650-23523669 | None:intergenic | 40.0% |
| ATCAGACCTTGACGTTGAAA+AGG | + | chr1_4:23523662-23523681 | Msa0156820:CDS | 40.0% | |
| ! | CAGATTCCTTTTCAACGTCA+AGG | - | chr1_4:23523671-23523690 | None:intergenic | 40.0% |
| !! | TGGAGATTGGTTTGAAAGCT+CGG | - | chr1_4:23523703-23523722 | None:intergenic | 40.0% |
| GGGCTACATTTCAAGGCTAA+AGG | + | chr1_4:23523594-23523613 | Msa0156820:CDS | 45.0% | |
| !! | GGAGATTGGTTTGAAAGCTC+GGG | - | chr1_4:23523702-23523721 | None:intergenic | 45.0% |
| GAGCTTTCAAACCAATCTCC+AGG | + | chr1_4:23523702-23523721 | Msa0156820:CDS | 45.0% | |
| CCAATGCCTATCTCATTGCT+TGG | - | chr1_4:23524280-23524299 | None:intergenic | 45.0% | |
| CCAAGCAATGAGATAGGCAT+TGG | + | chr1_4:23524277-23524296 | Msa0156820:CDS | 45.0% | |
| GGGCTACATTTCAAGGCTAA+AGG | + | chr1_4:23523594-23523613 | Msa0156820:CDS | 45.0% | |
| !! | GGAGATTGGTTTGAAAGCTC+GGG | - | chr1_4:23523702-23523721 | None:intergenic | 45.0% |
| GAGCTTTCAAACCAATCTCC+AGG | + | chr1_4:23523702-23523721 | Msa0156820:CDS | 45.0% | |
| CCAATGCCTATCTCATTGCT+TGG | - | chr1_4:23524280-23524299 | None:intergenic | 45.0% | |
| CCAAGCAATGAGATAGGCAT+TGG | + | chr1_4:23524277-23524296 | Msa0156820:CDS | 45.0% | |
| ! | CGCTTGTGGGCTACATTTCA+AGG | + | chr1_4:23523587-23523606 | Msa0156820:CDS | 50.0% |
| ! | CGCTTGTGGGCTACATTTCA+AGG | + | chr1_4:23523587-23523606 | Msa0156820:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_4 | gene | 23523525 | 23524345 | 23523525 | ID=Msa0156820;Name=Msa0156820 |
| chr1_4 | mRNA | 23523525 | 23524345 | 23523525 | ID=Msa0156820-mRNA-1;Parent=Msa0156820;Name=Msa0156820-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0|0|0|1|1|1|2|0|134 |
| chr1_4 | exon | 23523525 | 23523723 | 23523525 | ID=Msa0156820-mRNA-1:exon:8240;Parent=Msa0156820-mRNA-1 |
| chr1_4 | exon | 23524140 | 23524345 | 23524140 | ID=Msa0156820-mRNA-1:exon:8241;Parent=Msa0156820-mRNA-1 |
| chr1_4 | CDS | 23523525 | 23523723 | 23523525 | ID=Msa0156820-mRNA-1:cds;Parent=Msa0156820-mRNA-1 |
| chr1_4 | CDS | 23524140 | 23524345 | 23524140 | ID=Msa0156820-mRNA-1:cds;Parent=Msa0156820-mRNA-1 |
| Gene Sequence |
| Protein sequence |
