AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0158430

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0158430 MtrunA17_Chr1g0170581 90.984 244 22 0 1 244 1 244 2.35e-163 451
Msa0158430 MtrunA17_Chr1g0170571 87.149 249 24 1 1 249 1 241 4.99e-151 420
Msa0158430 MtrunA17_Chr1g0170591 81.526 249 38 1 1 249 1 241 1.35e-148 414
Msa0158430 MtrunA17_Chr7g0256671 58.566 251 85 7 1 241 1 242 1.21e-66 207
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0158430 AT3G56970.1 40.625 256 127 5 1 240 1 247 2.33e-54 176
Msa0158430 AT3G56980.1 40.304 263 127 6 1 242 1 254 1.77e-44 150
Msa0158430 AT2G41240.1 38.095 252 132 5 1 242 1 238 2.74e-44 149
Msa0158430 AT2G41240.2 37.698 252 132 6 1 242 1 237 7.95e-42 143
Msa0158430 AT5G04150.1 44.262 183 94 4 60 237 78 257 9.80e-36 128
Msa0158430 AT5G04150.2 45.399 163 84 2 60 219 78 238 3.05e-35 126

Find 50 sgRNAs with CRISPR-Local

Find 284 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCACCTGTTTCTGTAATTC+TGG 0.155913 1_4:+27705605 None:intergenic
TGTAACTATGCTTCTTCCTT+TGG 0.251062 1_4:+27705504 None:intergenic
CTTCTATAATTTGCATTTCC+AGG 0.251812 1_4:-27705312 Msa0158430:intron
GAGCTTCTCATTTAGAATTT+CGG 0.255939 1_4:+27701968 None:intergenic
TTCTGAGATCTTGATATGTT+TGG 0.287728 1_4:-27705393 Msa0158430:CDS
GTAACTATGCTTCTTCCTTT+GGG 0.307422 1_4:+27705505 None:intergenic
TGAGAAGCTCTTATCAATAA+TGG 0.319674 1_4:-27701955 Msa0158430:CDS
CTTTCTTCATCTCAGACCTT+TGG 0.339976 1_4:-27705344 Msa0158430:CDS
ATGAACTCTGATGCTGGTTA+TGG 0.347759 1_4:+27705974 None:intergenic
TGGACTCTTGAAATTGTTGC+TGG 0.347910 1_4:+27705638 None:intergenic
GATCGCGTTCACTAGCATTA+TGG 0.375108 1_4:+27705833 None:intergenic
TGATGCTGGTTATGGGATAT+TGG 0.393094 1_4:+27705982 None:intergenic
TAGAATAACCTCTTCTACTT+GGG 0.395863 1_4:+27705538 None:intergenic
TCTTCATCTCAGACCTTTGG+AGG 0.399010 1_4:-27705341 Msa0158430:CDS
AGTACCATTTGATCTGAAAC+AGG 0.414053 1_4:+27705763 None:intergenic
CATACCAGAATTACAGAAAC+AGG 0.417584 1_4:-27705609 Msa0158430:CDS
GCTCTTATCAATAATGGATA+AGG 0.434619 1_4:-27701949 Msa0158430:CDS
ATCTCAGACCTTTGGAGGGA+GGG 0.436300 1_4:-27705336 Msa0158430:CDS
TCTTCCTGTTTCAGATCAAA+TGG 0.439923 1_4:-27705767 Msa0158430:intron
TTAGAATAACCTCTTCTACT+TGG 0.448901 1_4:+27705537 None:intergenic
TGAACTCTGATGCTGGTTAT+GGG 0.461728 1_4:+27705975 None:intergenic
AACAATTGGATGGCCCTTTG+AGG 0.464251 1_4:-27706007 Msa0158430:CDS
CATATCAAGATCTCAGAAAG+TGG 0.493296 1_4:+27705398 None:intergenic
CATGCCAAGGTCACTGCTTG+TGG 0.499479 1_4:+27705870 None:intergenic
CATCTCAGACCTTTGGAGGG+AGG 0.501008 1_4:-27705337 Msa0158430:CDS
AAGAAAGATTAAGAAGCAAA+AGG 0.505181 1_4:+27705360 None:intergenic
CTTCATCTCAGACCTTTGGA+GGG 0.506754 1_4:-27705340 Msa0158430:CDS
TGGGATATTGGCTCCTCAAA+GGG 0.519126 1_4:+27705994 None:intergenic
ACAAATTATTGATCTTCTTG+CGG 0.529574 1_4:+27705809 None:intergenic
TTGATATGTTTGGAAAATGA+TGG 0.537710 1_4:-27705383 Msa0158430:CDS
ATGGGATATTGGCTCCTCAA+AGG 0.540741 1_4:+27705993 None:intergenic
AAGAAGCATAGTTACAACTC+TGG 0.551749 1_4:-27705497 Msa0158430:CDS
ACTTTCTCCCCAAGTAGAAG+AGG 0.556979 1_4:-27705546 Msa0158430:CDS
AGTGTAACATGATATCTGAA+TGG 0.558596 1_4:+27705433 None:intergenic
TGTAGAATGAACTCTGATGC+TGG 0.560692 1_4:+27705968 None:intergenic
TTATCAATAATGGATAAGGA+AGG 0.562276 1_4:-27701945 Msa0158430:CDS
CAATCCACAAGCAGTGACCT+TGG 0.569748 1_4:-27705874 Msa0158430:CDS
AGAATAACCTCTTCTACTTG+GGG 0.586846 1_4:+27705539 None:intergenic
ACTAGTTAAGAGAAAAGAAG+AGG 0.589176 1_4:-27705579 Msa0158430:CDS
GTCACTGCTTGTGGATTGTG+AGG 0.591686 1_4:+27705879 None:intergenic
ATAGAAGACCCTCCCTCCAA+AGG 0.594272 1_4:+27705328 None:intergenic
TATCAATAATGGATAAGGAA+GGG 0.600012 1_4:-27701944 Msa0158430:CDS
CACAAGCAGTGACCTTGGCA+TGG 0.602895 1_4:-27705869 Msa0158430:CDS
TTCTAAGCAAAGAATCCCAA+AGG 0.620328 1_4:-27705520 Msa0158430:CDS
CATCCAATTGTTGAAAACAC+AGG 0.625565 1_4:+27706018 None:intergenic
CAAGCTTCTTAACCATGCCA+AGG 0.655760 1_4:+27705857 None:intergenic
ACCAGAATTACAGAAACAGG+TGG 0.656125 1_4:-27705606 Msa0158430:CDS
GAATTACAGAAACAGGTGGA+AGG 0.659206 1_4:-27705602 Msa0158430:CDS
CCAATTGTTGAAAACACAGG+AGG 0.684063 1_4:+27706021 None:intergenic
TCACTGCTTGTGGATTGTGA+GGG 0.698792 1_4:+27705880 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAAAAGAAAAAAAAAAA+AGG - chr1_4:27703750-27703769 Msa0158430:intron 10.0%
!!! ACAATTTTTTTTTTAGTTAA+GGG + chr1_4:27703825-27703844 None:intergenic 10.0%
!!! AACAATTTTTTTTTTAGTTA+AGG + chr1_4:27703826-27703845 None:intergenic 10.0%
!!! AACTAAAAAAAAAATTGTTT+TGG - chr1_4:27703827-27703846 Msa0158430:intron 10.0%
!!! GTTTTTTTGAATTTATTTAA+CGG - chr1_4:27704589-27704608 Msa0158430:intron 10.0%
!!! CTAAAAAAAAAAAATTGTTT+TGG + chr1_4:27704653-27704672 None:intergenic 10.0%
!!! CAATTTTTTTTTTTTAGTTA+AGG - chr1_4:27704656-27704675 Msa0158430:intron 10.0%
!! AGTATAAAATTTCATTTAAT+AGG - chr1_4:27705302-27705321 Msa0158430:intron 10.0%
!! GTATAAAATTTCATTTAATA+GGG - chr1_4:27705303-27705322 Msa0158430:intron 10.0%
!! TATAAAATTTCATTTAATAG+GGG - chr1_4:27705304-27705323 Msa0158430:intron 10.0%
!! ATTAACAAAATCATATAGAA+GGG + chr1_4:27702227-27702246 None:intergenic 15.0%
!! AATTAACAAAATCATATAGA+AGG + chr1_4:27702228-27702247 None:intergenic 15.0%
!! TTTGTCATTGTAATATTTAT+AGG + chr1_4:27703625-27703644 None:intergenic 15.0%
!! AAAAAAATCATTTGCATAAT+GGG + chr1_4:27703705-27703724 None:intergenic 15.0%
!! AAAAAAAATCATTTGCATAA+TGG + chr1_4:27703706-27703725 None:intergenic 15.0%
!!! AATGATTTTTTTTTATGAAG+AGG - chr1_4:27703713-27703732 Msa0158430:intron 15.0%
!!! GTTTTTTTGAGTTTATTTAA+CGG + chr1_4:27703891-27703910 None:intergenic 15.0%
!! GATACTAAATAAATACTTAA+GGG - chr1_4:27703970-27703989 Msa0158430:intron 15.0%
!!! AAAAAACTTAAATGTTCTTT+TGG + chr1_4:27703997-27704016 None:intergenic 15.0%
!!! TTGTCTTTTAAGTATTTATT+TGG - chr1_4:27704503-27704522 Msa0158430:intron 15.0%
!! ATAAATTCAAAAAAACGTTA+GGG + chr1_4:27704585-27704604 None:intergenic 15.0%
!! AATAAATTCAAAAAAACGTT+AGG + chr1_4:27704586-27704605 None:intergenic 15.0%
!! GTCTTTATATTTAAAAAGTA+TGG - chr1_4:27705200-27705219 Msa0158430:intron 15.0%
!! TGATTAATCATTTGAATAAT+GGG + chr1_4:27705741-27705760 None:intergenic 15.0%
!! TATAAATTTATCACTGCATA+TGG + chr1_4:27702790-27702809 None:intergenic 20.0%
!! AAACAATGAAACAAAAATGT+AGG - chr1_4:27703660-27703679 Msa0158430:intron 20.0%
!! GATACCAAATATATAGTTAA+GGG + chr1_4:27703794-27703813 None:intergenic 20.0%
!! AATAAACTCAAAAAAACGTT+AGG - chr1_4:27703894-27703913 Msa0158430:intron 20.0%
!! ATAAACTCAAAAAAACGTTA+GGG - chr1_4:27703895-27703914 Msa0158430:intron 20.0%
!!! GTATTTATTTAGTATCGTTT+TGG + chr1_4:27703966-27703985 None:intergenic 20.0%
!! CGATACTAAATAAATACTTA+AGG - chr1_4:27703969-27703988 Msa0158430:intron 20.0%
!!! TGTTATTGATTGTTATTGTT+GGG + chr1_4:27704103-27704122 None:intergenic 20.0%
!!! ATGTTATTGATTGTTATTGT+TGG + chr1_4:27704104-27704123 None:intergenic 20.0%
!! AAAAGACAAAAAGAACATTT+AGG + chr1_4:27704492-27704511 None:intergenic 20.0%
!!! TTTTGAATTTATTTAACGGA+AGG - chr1_4:27704593-27704612 Msa0158430:intron 20.0%
!! TTTGAATTTATTTAACGGAA+GGG - chr1_4:27704594-27704613 Msa0158430:intron 20.0%
!! ATAATAACATCATTATCGTT+TGG - chr1_4:27705095-27705114 Msa0158430:intron 20.0%
!!! ACTTTAATTTGTAGATGTTA+AGG + chr1_4:27705168-27705187 None:intergenic 20.0%
!! CAAAAATTTAACAAATAACG+CGG - chr1_4:27705365-27705384 Msa0158430:CDS 20.0%
!! TTTATTAAAATCAATGTGGA+CGG + chr1_4:27705647-27705666 None:intergenic 20.0%
!! GTGATTAATCATTTGAATAA+TGG + chr1_4:27705742-27705761 None:intergenic 20.0%
!!! TATTAATTGATAGTTCCTTT+TGG - chr1_4:27705929-27705948 Msa0158430:CDS 20.0%
!!! ATTAATTGATAGTTCCTTTT+GGG - chr1_4:27705930-27705949 Msa0158430:CDS 20.0%
!!! TAATTTTCATGATCAAGTTG+AGG - chr1_4:27702033-27702052 Msa0158430:intron 25.0%
! ACAAATTATTGATCTTCTTG+CGG + chr1_4:27702150-27702169 None:intergenic 25.0%
!! GTAATTCTGGTATGTATTTT+TGG + chr1_4:27702341-27702360 None:intergenic 25.0%
! TTGATATGTTTGGAAAATGA+TGG - chr1_4:27702573-27702592 Msa0158430:intron 25.0%
! AAGAAAGATTAAGAAGCAAA+AGG + chr1_4:27702599-27702618 None:intergenic 25.0%
! AAGACTTTACTCAACAATAT+TGG + chr1_4:27702714-27702733 None:intergenic 25.0%
! AACCATTAGAAAAGATTTAG+AGG + chr1_4:27702992-27703011 None:intergenic 25.0%
!! GTTTCTCTAATAGTTTTCTT+AGG - chr1_4:27703009-27703028 Msa0158430:intron 25.0%
!!! TAGAAAAGTTGGTAGAAAAT+AGG + chr1_4:27703134-27703153 None:intergenic 25.0%
! CGATACCAAATATATAGTTA+AGG + chr1_4:27704687-27704706 None:intergenic 25.0%
!! TTTGAGTTTATTTAACGGAA+GGG + chr1_4:27703886-27703905 None:intergenic 25.0%
!!! TTTTGAGTTTATTTAACGGA+AGG + chr1_4:27703887-27703906 None:intergenic 25.0%
!!! TGAATTCTGTGTTTTTTTTC+TGG - chr1_4:27704411-27704430 Msa0158430:intron 25.0%
!! GTATTTATTTGGTATCGTTT+TGG - chr1_4:27704514-27704533 Msa0158430:intron 25.0%
! CGATACCAAATATATAGTTA+GGG - chr1_4:27704687-27704706 Msa0158430:intron 25.0%
! GATACCAAATATATAGTTAG+GGG - chr1_4:27704688-27704707 Msa0158430:intron 25.0%
!!! AGGCTTTATTAAAATCAATG+TGG + chr1_4:27705651-27705670 None:intergenic 25.0%
!!! TTGATTTTAATAAAGCCTTC+CGG - chr1_4:27705653-27705672 Msa0158430:CDS 25.0%
! TCACTTGACATTTATTTGTT+GGG - chr1_4:27705702-27705721 Msa0158430:intron 25.0%
!!! TTATCAATAATGGATAAGGA+AGG - chr1_4:27706011-27706030 Msa0158430:CDS 25.0%
!!! TATCAATAATGGATAAGGAA+GGG - chr1_4:27706012-27706031 Msa0158430:CDS 25.0%
ACTAGTTAAGAGAAAAGAAG+AGG - chr1_4:27702377-27702396 Msa0158430:intron 30.0%
TAGAATAACCTCTTCTACTT+GGG + chr1_4:27702421-27702440 None:intergenic 30.0%
TTAGAATAACCTCTTCTACT+TGG + chr1_4:27702422-27702441 None:intergenic 30.0%
!! GTTTTGTAGTTTCAAGTAGT+AGG - chr1_4:27702481-27702500 Msa0158430:intron 30.0%
AGTGTAACATGATATCTGAA+TGG + chr1_4:27702526-27702545 None:intergenic 30.0%
!! TTCTGAGATCTTGATATGTT+TGG - chr1_4:27702563-27702582 Msa0158430:intron 30.0%
! CTTCTATAATTTGCATTTCC+AGG - chr1_4:27702644-27702663 Msa0158430:intron 30.0%
TCTTTGATAGAACATTATGG+CGG + chr1_4:27702945-27702964 None:intergenic 30.0%
TGGTCTTTGATAGAACATTA+TGG + chr1_4:27702948-27702967 None:intergenic 30.0%
GTTCTATCAAAGACCATATT+TGG - chr1_4:27702952-27702971 Msa0158430:intron 30.0%
! GACCTCTAAATCTTTTCTAA+TGG - chr1_4:27702987-27703006 Msa0158430:intron 30.0%
! TCTACCAACTTTTCTACTAT+AGG - chr1_4:27703138-27703157 Msa0158430:intron 30.0%
ATTTATCACTGCATATGGTA+CGG + chr1_4:27703298-27703317 None:intergenic 30.0%
TTTATCTATGACCAATGCAT+TGG - chr1_4:27703587-27703606 Msa0158430:intron 30.0%
ACAAAAAATAGCCAATGCAT+TGG + chr1_4:27703601-27703620 None:intergenic 30.0%
! AAAAGGCTTAAATGCTCTTT+TGG - chr1_4:27703767-27703786 Msa0158430:intron 30.0%
TGGTCCCTTAACTATATATT+TGG - chr1_4:27703787-27703806 Msa0158430:intron 30.0%
CTATATATTTGGTATCGCTT+TGG - chr1_4:27703798-27703817 Msa0158430:intron 30.0%
! TTGTTGTAAGTTGTTATTGC+TGG + chr1_4:27704054-27704073 None:intergenic 30.0%
ATCAATAACAACAACAACAC+AGG - chr1_4:27704141-27704160 Msa0158430:intron 30.0%
! CAAAAAAACCAACCCATTTT+CGG - chr1_4:27704177-27704196 Msa0158430:intron 30.0%
! ATGCTTTTAGATCCGAAAAT+GGG + chr1_4:27704192-27704211 None:intergenic 30.0%
! TATGCTTTTAGATCCGAAAA+TGG + chr1_4:27704193-27704212 None:intergenic 30.0%
!!! TTCTATTGCTGCAGTTTTTT+CGG - chr1_4:27704260-27704279 Msa0158430:intron 30.0%
CTATATATTTGGTATCGCTT+TGG + chr1_4:27703798-27703817 None:intergenic 30.0%
GCGATACCAAATATATAGTT+AGG - chr1_4:27704686-27704705 Msa0158430:intron 30.0%
!!! GGGCAATCTATTATTTTAAG+AGG - chr1_4:27705324-27705343 Msa0158430:CDS 30.0%
TGATTACAGATCTATCATCA+TGG + chr1_4:27705466-27705485 None:intergenic 30.0%
GAACTTTCAGCTATTTGAAA+GGG + chr1_4:27705510-27705529 None:intergenic 30.0%
CTTTCAAATAGCTGAAAGTT+CGG - chr1_4:27705509-27705528 Msa0158430:CDS 30.0%
! AATTGGATTCATGGTTTTTC+TGG + chr1_4:27705563-27705582 None:intergenic 30.0%
!! AGGTCTTTTGAGAAACAATA+GGG + chr1_4:27705588-27705607 None:intergenic 30.0%
GTCACTTGACATTTATTTGT+TGG - chr1_4:27705701-27705720 Msa0158430:intron 30.0%
! GAGCTTCTCATTTAGAATTT+CGG + chr1_4:27705991-27706010 None:intergenic 30.0%
TGAGAAGCTCTTATCAATAA+TGG - chr1_4:27706001-27706020 Msa0158430:CDS 30.0%
!! GCTCTTATCAATAATGGATA+AGG - chr1_4:27706007-27706026 Msa0158430:CDS 30.0%
CATCCAATTGTTGAAAACAC+AGG + chr1_4:27701941-27701960 None:intergenic 35.0%
! CTGTGTTTTCAACAATTGGA+TGG - chr1_4:27701939-27701958 Msa0158430:CDS 35.0%
TCTTCCTGTTTCAGATCAAA+TGG - chr1_4:27702189-27702208 Msa0158430:intron 35.0%
! AGTACCATTTGATCTGAAAC+AGG + chr1_4:27702196-27702215 None:intergenic 35.0%
CATACCAGAATTACAGAAAC+AGG - chr1_4:27702347-27702366 Msa0158430:intron 35.0%
AGAATAACCTCTTCTACTTG+GGG + chr1_4:27702420-27702439 None:intergenic 35.0%
TTCTAAGCAAAGAATCCCAA+AGG - chr1_4:27702436-27702455 Msa0158430:intron 35.0%
GTAACTATGCTTCTTCCTTT+GGG + chr1_4:27702454-27702473 None:intergenic 35.0%
TGTAACTATGCTTCTTCCTT+TGG + chr1_4:27702455-27702474 None:intergenic 35.0%
! AAGAAGCATAGTTACAACTC+TGG - chr1_4:27702459-27702478 Msa0158430:intron 35.0%
CATATCAAGATCTCAGAAAG+TGG + chr1_4:27702561-27702580 None:intergenic 35.0%
! TCCAGGTGTGTTAATTTTAC+AGG - chr1_4:27702661-27702680 Msa0158430:intron 35.0%
TCCTGTAAAATTAACACACC+TGG + chr1_4:27702665-27702684 None:intergenic 35.0%
! CAATGAGTTGGATCCAAATA+TGG + chr1_4:27702968-27702987 None:intergenic 35.0%
AGATTTAGAGGTCAATGAGT+TGG + chr1_4:27702980-27702999 None:intergenic 35.0%
AAGAAGAGTAGATCAAATGG+AGG + chr1_4:27703064-27703083 None:intergenic 35.0%
GGTAAGAAGAGTAGATCAAA+TGG + chr1_4:27703067-27703086 None:intergenic 35.0%
TTGATCTACTCTTCTTACCA+CGG - chr1_4:27703068-27703087 Msa0158430:intron 35.0%
GAAAATAGGCTTAGATGGTT+TGG + chr1_4:27703120-27703139 None:intergenic 35.0%
TGGTAGAAAATAGGCTTAGA+TGG + chr1_4:27703125-27703144 None:intergenic 35.0%
AGCACCTATAGTAGAAAAGT+TGG + chr1_4:27703145-27703164 None:intergenic 35.0%
AGACTAGACATGATAGGATT+AGG + chr1_4:27703197-27703216 None:intergenic 35.0%
! TTTATCTCCGTTACCTGTTT+TGG - chr1_4:27703859-27703878 Msa0158430:intron 35.0%
! TTTTAGATCCGAAAATGGGT+TGG + chr1_4:27704188-27704207 None:intergenic 35.0%
TGGTCCCCTAACTATATATT+TGG + chr1_4:27704695-27704714 None:intergenic 35.0%
!!! TTTTGGCTTGAATGCTCTTT+TGG + chr1_4:27704715-27704734 None:intergenic 35.0%
! AAGAGAATAAATGTGCTGAC+TGG + chr1_4:27704860-27704879 None:intergenic 35.0%
CAACTTTGCTTTCTCTCATT+AGG + chr1_4:27704932-27704951 None:intergenic 35.0%
!!! TCCAGAAATTTTGACTTACG+AGG - chr1_4:27705014-27705033 Msa0158430:intron 35.0%
!!! CCAGAAATTTTGACTTACGA+GGG - chr1_4:27705015-27705034 Msa0158430:intron 35.0%
AAATAACGCGGAGAAATTAG+TGG - chr1_4:27705377-27705396 Msa0158430:CDS 35.0%
AATAACGCGGAGAAATTAGT+GGG - chr1_4:27705378-27705397 Msa0158430:CDS 35.0%
CGAACTTTCAGCTATTTGAA+AGG + chr1_4:27705511-27705530 None:intergenic 35.0%
!! GTCTTTTGAGAAACAATAGG+GGG + chr1_4:27705586-27705605 None:intergenic 35.0%
!! GGTCTTTTGAGAAACAATAG+GGG + chr1_4:27705587-27705606 None:intergenic 35.0%
!! GAGGTCTTTTGAGAAACAAT+AGG + chr1_4:27705589-27705608 None:intergenic 35.0%
CCAATTGTTGAAAACACAGG+AGG + chr1_4:27701938-27701957 None:intergenic 40.0%
! CCTCCTGTGTTTTCAACAAT+TGG - chr1_4:27701935-27701954 Msa0158430:CDS 40.0%
! TGATGCTGGTTATGGGATAT+TGG + chr1_4:27701977-27701996 None:intergenic 40.0%
! TGAACTCTGATGCTGGTTAT+GGG + chr1_4:27701984-27702003 None:intergenic 40.0%
! ATGAACTCTGATGCTGGTTA+TGG + chr1_4:27701985-27702004 None:intergenic 40.0%
TGTAGAATGAACTCTGATGC+TGG + chr1_4:27701991-27702010 None:intergenic 40.0%
TGGACTCTTGAAATTGTTGC+TGG + chr1_4:27702321-27702340 None:intergenic 40.0%
ACCAGAATTACAGAAACAGG+TGG - chr1_4:27702350-27702369 Msa0158430:intron 40.0%
!! TCCACCTGTTTCTGTAATTC+TGG + chr1_4:27702354-27702373 None:intergenic 40.0%
GAATTACAGAAACAGGTGGA+AGG - chr1_4:27702354-27702373 Msa0158430:intron 40.0%
CTTTCTTCATCTCAGACCTT+TGG - chr1_4:27702612-27702631 Msa0158430:intron 40.0%
AGTCAGGTTAAAAGAGGTAG+AGG + chr1_4:27703039-27703058 None:intergenic 40.0%
ACATCATTAGAGAGAGAGTG+GGG + chr1_4:27703170-27703189 None:intergenic 40.0%
GACATCATTAGAGAGAGAGT+GGG + chr1_4:27703171-27703190 None:intergenic 40.0%
TGACATCATTAGAGAGAGAG+TGG + chr1_4:27703172-27703191 None:intergenic 40.0%
GTGGTAAGACTAGACATGAT+AGG + chr1_4:27703203-27703222 None:intergenic 40.0%
! AACACACCAACCCAGATTTT+GGG - chr1_4:27703939-27703958 Msa0158430:intron 40.0%
!! TCGTTTTGGTCCCAAAATCT+GGG + chr1_4:27704528-27704547 None:intergenic 40.0%
!! ATCGTTTTGGTCCCAAAATC+TGG + chr1_4:27704527-27704546 None:intergenic 40.0%
! TTGCTGGTGATGAATGATGA+AGG + chr1_4:27704038-27704057 None:intergenic 40.0%
! TGGGTTGTTGTTATTGTTGC+TGG + chr1_4:27704084-27704103 None:intergenic 40.0%
!!! TTTTTTCGGTACAGTGGATG+AGG - chr1_4:27704274-27704293 Msa0158430:intron 40.0%
GGGGAAACGAAAATGAAGAT+GGG - chr1_4:27704302-27704321 Msa0158430:intron 40.0%
GGGAAACGAAAATGAAGATG+GGG - chr1_4:27704303-27704322 Msa0158430:intron 40.0%
!! TCGTTTTGGTCCCAAAATCT+AGG - chr1_4:27704528-27704547 Msa0158430:intron 40.0%
TTTGGTCCCAAAATCTAGGT+TGG - chr1_4:27704532-27704551 Msa0158430:intron 40.0%
TTGGTCCCAAAATCTAGGTT+GGG - chr1_4:27704533-27704552 Msa0158430:intron 40.0%
! AACACCCCAACCTAGATTTT+GGG + chr1_4:27704541-27704560 None:intergenic 40.0%
TTAACGGAAGGGACTAAAAC+AGG - chr1_4:27704605-27704624 Msa0158430:intron 40.0%
GAAGGGACTAAAACAGGTAA+CGG - chr1_4:27704611-27704630 Msa0158430:intron 40.0%
!!! CGGATCGGCTAGTTTTTTTT+TGG + chr1_4:27704732-27704751 None:intergenic 40.0%
AGCAGGATGTTATCCAATTG+AGG - chr1_4:27704780-27704799 Msa0158430:intron 40.0%
AGGATGTTATCCAATTGAGG+AGG - chr1_4:27704783-27704802 Msa0158430:intron 40.0%
AGGAGAATTCCACATCTTCA+AGG - chr1_4:27704803-27704822 Msa0158430:intron 40.0%
ATTCCACATCTTCAAGGAGT+CGG - chr1_4:27704809-27704828 Msa0158430:intron 40.0%
!! GAATAAATGTGCTGACTGGT+TGG + chr1_4:27704856-27704875 None:intergenic 40.0%
GAAGCTTTCTTCACTTCCAT+GGG + chr1_4:27704910-27704929 None:intergenic 40.0%
AGAGAAAGCAAAGTTGCATG+AGG - chr1_4:27704936-27704955 Msa0158430:intron 40.0%
CCCTCGTAAGTCAAAATTTC+TGG + chr1_4:27705018-27705037 None:intergenic 40.0%
ATAACGCGGAGAAATTAGTG+GGG - chr1_4:27705379-27705398 Msa0158430:CDS 40.0%
TATTTGAAAGGGTCTTCAGC+TGG + chr1_4:27705499-27705518 None:intergenic 40.0%
AATAGGGGGAATTGGATTCA+TGG + chr1_4:27705572-27705591 None:intergenic 40.0%
TGAGAAACAATAGGGGGAAT+TGG + chr1_4:27705580-27705599 None:intergenic 40.0%
!! AATCCTGGTAATGCAGGTTT+TGG + chr1_4:27705611-27705630 None:intergenic 40.0%
! CTTGATAATCCTGGTAATGC+AGG + chr1_4:27705617-27705636 None:intergenic 40.0%
! GGATCTTCTCTTGATAATCC+TGG + chr1_4:27705626-27705645 None:intergenic 40.0%
TTAATAAAGCCTTCCGGTAG+AGG - chr1_4:27705659-27705678 Msa0158430:intron 40.0%
!! GAATAATGGGTTCATGCTCT+TGG + chr1_4:27705728-27705747 None:intergenic 40.0%
TATTTATGTGCCAGTACTCC+AGG - chr1_4:27705876-27705895 Msa0158430:CDS 40.0%
ATTTATGTGCCAGTACTCCA+GGG - chr1_4:27705877-27705896 Msa0158430:CDS 40.0%
!!! GTTCCTTTTGGGTTTTTTGC+AGG - chr1_4:27705941-27705960 Msa0158430:CDS 40.0%
CTACCTGCAAAAAACCCAAA+AGG + chr1_4:27705947-27705966 None:intergenic 40.0%
AACAATTGGATGGCCCTTTG+AGG - chr1_4:27701949-27701968 Msa0158430:CDS 45.0%
!! TGGGATATTGGCTCCTCAAA+GGG + chr1_4:27701965-27701984 None:intergenic 45.0%
!! ATGGGATATTGGCTCCTCAA+AGG + chr1_4:27701966-27701985 None:intergenic 45.0%
! TCACTGCTTGTGGATTGTGA+GGG + chr1_4:27702079-27702098 None:intergenic 45.0%
CAAGCTTCTTAACCATGCCA+AGG + chr1_4:27702102-27702121 None:intergenic 45.0%
GATCGCGTTCACTAGCATTA+TGG + chr1_4:27702126-27702145 None:intergenic 45.0%
ACTTTCTCCCCAAGTAGAAG+AGG - chr1_4:27702410-27702429 Msa0158430:intron 45.0%
TCTTCATCTCAGACCTTTGG+AGG - chr1_4:27702615-27702634 Msa0158430:intron 45.0%
CTTCATCTCAGACCTTTGGA+GGG - chr1_4:27702616-27702635 Msa0158430:intron 45.0%
AACGCCTTATCCCACTAAGT+GGG - chr1_4:27702898-27702917 Msa0158430:intron 45.0%
GAGGAGAGTCAGGTTAAAAG+AGG + chr1_4:27703045-27703064 None:intergenic 45.0%
AGATCAAATGGAGGAGAGTC+AGG + chr1_4:27703055-27703074 None:intergenic 45.0%
CATCATTAGAGAGAGAGTGG+GGG + chr1_4:27703169-27703188 None:intergenic 45.0%
CGAGAGTAAAGTAAGCGTAG+CGG + chr1_4:27703250-27703269 None:intergenic 45.0%
CGCTTACTTTACTCTCGTGT+TGG - chr1_4:27703253-27703272 Msa0158430:intron 45.0%
! TGCATATGGTACGGAGTGTT+GGG + chr1_4:27703289-27703308 None:intergenic 45.0%
GAAGGGACCAAAACAGGTAA+CGG + chr1_4:27703869-27703888 None:intergenic 45.0%
TTAACGGAAGGGACCAAAAC+AGG + chr1_4:27703875-27703894 None:intergenic 45.0%
! CAACACACCAACCCAGATTT+TGG - chr1_4:27703938-27703957 Msa0158430:intron 45.0%
TTTGGTCCCAAAATCTGGGT+TGG + chr1_4:27703948-27703967 None:intergenic 45.0%
AAAGAACAGCTGCACAGAGA+TGG + chr1_4:27704238-27704257 None:intergenic 45.0%
!!! CTGCAGTTTTTTCGGTACAG+TGG - chr1_4:27704268-27704287 Msa0158430:intron 45.0%
GGGGGAAACGAAAATGAAGA+TGG - chr1_4:27704301-27704320 Msa0158430:intron 45.0%
TGGTCCCAAAATCTAGGTTG+GGG - chr1_4:27704534-27704553 Msa0158430:intron 45.0%
! CAACACCCCAACCTAGATTT+TGG + chr1_4:27704542-27704561 None:intergenic 45.0%
!!! CGAATACCTCTGGGGTTTTT+AGG + chr1_4:27704758-27704777 None:intergenic 45.0%
GTGGAATTCTCCTCCTCAAT+TGG + chr1_4:27704796-27704815 None:intergenic 45.0%
!! ATACCGACTCCTTGAAGATG+TGG + chr1_4:27704815-27704834 None:intergenic 45.0%
! GCTGACTGGTTGGCTAAATT+TGG + chr1_4:27704846-27704865 None:intergenic 45.0%
TGACTAAATGAGACCACCCA+TGG - chr1_4:27704891-27704910 Msa0158430:intron 45.0%
GGAAGCTTTCTTCACTTCCA+TGG + chr1_4:27704911-27704930 None:intergenic 45.0%
AGATCCAAAGCAGTCAGTGA+CGG - chr1_4:27704990-27705009 Msa0158430:intron 45.0%
TAACGCGGAGAAATTAGTGG+GGG - chr1_4:27705380-27705399 Msa0158430:CDS 45.0%
!! ATCCACTTGTAGTGGATTGG+AGG + chr1_4:27705412-27705431 None:intergenic 45.0%
! AAGTGACTAGTCAAGTGCGT+TGG + chr1_4:27705688-27705707 None:intergenic 45.0%
TTTATGTGCCAGTACTCCAG+GGG - chr1_4:27705878-27705897 Msa0158430:CDS 45.0%
!!! AATTTTTTTTTTTTAGTTAA+GGG - chr1_4:27704657-27704676 Msa0158430:intron 5.0%
!!! TTTTTTTAAAAGAAAAATAT+TGG - chr1_4:27705812-27705831 Msa0158430:CDS 5.0%
! GTCACTGCTTGTGGATTGTG+AGG + chr1_4:27702080-27702099 None:intergenic 50.0%
CAATCCACAAGCAGTGACCT+TGG - chr1_4:27702082-27702101 Msa0158430:intron 50.0%
! ATCTCAGACCTTTGGAGGGA+GGG - chr1_4:27702620-27702639 Msa0158430:intron 50.0%
ATAGAAGACCCTCCCTCCAA+AGG + chr1_4:27702631-27702650 None:intergenic 50.0%
CAACGCCTTATCCCACTAAG+TGG - chr1_4:27702897-27702916 Msa0158430:intron 50.0%
ACGCCTTATCCCACTAAGTG+GGG - chr1_4:27702899-27702918 Msa0158430:intron 50.0%
CTTATCCCACTAAGTGGGGT+CGG - chr1_4:27702903-27702922 Msa0158430:intron 50.0%
TCTTCTTACCACGGCGTCTA+CGG - chr1_4:27703077-27703096 Msa0158430:intron 50.0%
AGGATGTTGCGTTGGATGAG+TGG + chr1_4:27703222-27703241 None:intergenic 50.0%
! AAGTAAGCGTAGCGGAGATG+AGG + chr1_4:27703242-27703261 None:intergenic 50.0%
!! ATATGGTACGGAGTGTTGGG+CGG + chr1_4:27703286-27703305 None:intergenic 50.0%
CTGCATATGGTACGGAGTGT+TGG + chr1_4:27703290-27703309 None:intergenic 50.0%
TCGGTACAGTGGATGAGGTT+TGG - chr1_4:27704279-27704298 Msa0158430:intron 50.0%
CGGTACAGTGGATGAGGTTT+GGG - chr1_4:27704280-27704299 Msa0158430:intron 50.0%
GGTACAGTGGATGAGGTTTG+GGG - chr1_4:27704281-27704300 Msa0158430:intron 50.0%
GTACAGTGGATGAGGTTTGG+GGG - chr1_4:27704282-27704301 Msa0158430:intron 50.0%
TACAGTGGATGAGGTTTGGG+GGG - chr1_4:27704283-27704302 Msa0158430:intron 50.0%
TGAAGATGGGGAGAGAGAGA+TGG - chr1_4:27704315-27704334 Msa0158430:intron 50.0%
ATGGGGAGAGAGAGATGGAA+TGG - chr1_4:27704320-27704339 Msa0158430:intron 50.0%
TGGGGAGAGAGAGATGGAAT+GGG - chr1_4:27704321-27704340 Msa0158430:intron 50.0%
TCCTGCTTGCGAATACCTCT+GGG + chr1_4:27704767-27704786 None:intergenic 50.0%
ATCCTGCTTGCGAATACCTC+TGG + chr1_4:27704768-27704787 None:intergenic 50.0%
GCTTTCTTCACTTCCATGGG+TGG + chr1_4:27704907-27704926 None:intergenic 50.0%
TGCCTCCAATCCACTACAAG+TGG - chr1_4:27705407-27705426 Msa0158430:CDS 50.0%
!! CGGATCCACTTGTAGTGGAT+TGG + chr1_4:27705415-27705434 None:intergenic 50.0%
TCTCAGACTGCTTTCAGGCA+CGG + chr1_4:27705435-27705454 None:intergenic 50.0%
CTGACTCTCAGACTGCTTTC+AGG + chr1_4:27705440-27705459 None:intergenic 50.0%
!! CCTGGTAATGCAGGTTTTGG+AGG + chr1_4:27705608-27705627 None:intergenic 50.0%
CCTCCAAAACCTGCATTACC+AGG - chr1_4:27705605-27705624 Msa0158430:CDS 50.0%
AGTGCGTTGGAATCCTCTAC+CGG + chr1_4:27705675-27705694 None:intergenic 50.0%
CATGCCAAGGTCACTGCTTG+TGG + chr1_4:27702089-27702108 None:intergenic 55.0%
CACAAGCAGTGACCTTGGCA+TGG - chr1_4:27702087-27702106 Msa0158430:intron 55.0%
! CATCTCAGACCTTTGGAGGG+AGG - chr1_4:27702619-27702638 Msa0158430:intron 55.0%
CGACCCCACTTAGTGGGATA+AGG + chr1_4:27702905-27702924 None:intergenic 55.0%
TGTAGCCGACCCCACTTAGT+GGG + chr1_4:27702911-27702930 None:intergenic 55.0%
ATGTAGCCGACCCCACTTAG+TGG + chr1_4:27702912-27702931 None:intergenic 55.0%
ACTAAGTGGGGTCGGCTACA+TGG - chr1_4:27702911-27702930 Msa0158430:intron 55.0%
CTTCTTACCACGGCGTCTAC+GGG - chr1_4:27703078-27703097 Msa0158430:intron 55.0%
CGGAGATGAGGATGTTGCGT+TGG + chr1_4:27703230-27703249 None:intergenic 55.0%
ACAGCTGCACAGAGATGGAG+AGG + chr1_4:27704233-27704252 None:intergenic 55.0%
GGGGAGAGAGAGATGGAATG+GGG - chr1_4:27704322-27704341 Msa0158430:intron 55.0%
GGGAGAGAGAGATGGAATGG+GGG - chr1_4:27704323-27704342 Msa0158430:intron 55.0%
GGAGAGAGAGATGGAATGGG+GGG - chr1_4:27704324-27704343 Msa0158430:intron 55.0%
AGAGAGATGGAATGGGGGGT+AGG - chr1_4:27704328-27704347 Msa0158430:intron 55.0%
!! GGGGTTTTTAGGCAGCGGAT+CGG + chr1_4:27704747-27704766 None:intergenic 55.0%
!!! CCTCTGGGGTTTTTAGGCAG+CGG + chr1_4:27704752-27704771 None:intergenic 55.0%
CCTGCTTGCGAATACCTCTG+GGG + chr1_4:27704766-27704785 None:intergenic 55.0%
! GGATCCGTCACTGACTGCTT+TGG + chr1_4:27704997-27705016 None:intergenic 55.0%
AGGCACGGATCCACTTGTAG+TGG + chr1_4:27705420-27705439 None:intergenic 55.0%
AAAGGGTCTTCAGCTGGCGT+GGG + chr1_4:27705493-27705512 None:intergenic 55.0%
CGTTGGAATCCTCTACCGGA+AGG + chr1_4:27705671-27705690 None:intergenic 55.0%
AGTACTCCAGGGGAGAGATG+CGG - chr1_4:27705888-27705907 Msa0158430:CDS 55.0%
CCGCTGCCTAAAAACCCCAG+AGG - chr1_4:27704749-27704768 Msa0158430:intron 60.0%
!! CCCCAGAGGTATTCGCAAGC+AGG - chr1_4:27704763-27704782 Msa0158430:intron 60.0%
GAAAGGGTCTTCAGCTGGCG+TGG + chr1_4:27705494-27705513 None:intergenic 60.0%
GCATCTCTCCCCTGGAGTAC+TGG + chr1_4:27705889-27705908 None:intergenic 60.0%
AGTACTCCGCATCTCTCCCC+TGG + chr1_4:27705897-27705916 None:intergenic 60.0%
GAGAAGACCCGTAGACGCCG+TGG + chr1_4:27703088-27703107 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 27701920 27706058 27701920 ID=Msa0158430;Name=Msa0158430
chr1_4 mRNA 27701920 27706058 27701920 ID=Msa0158430-mRNA-1;Parent=Msa0158430;Name=Msa0158430-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|3|0|249
chr1_4 exon 27705768 27706058 27705768 ID=Msa0158430-mRNA-1:exon:9025;Parent=Msa0158430-mRNA-1
chr1_4 exon 27705313 27705675 27705313 ID=Msa0158430-mRNA-1:exon:9024;Parent=Msa0158430-mRNA-1
chr1_4 exon 27701920 27702015 27701920 ID=Msa0158430-mRNA-1:exon:9023;Parent=Msa0158430-mRNA-1
chr1_4 CDS 27705768 27706058 27705768 ID=Msa0158430-mRNA-1:cds;Parent=Msa0158430-mRNA-1
chr1_4 CDS 27705313 27705675 27705313 ID=Msa0158430-mRNA-1:cds;Parent=Msa0158430-mRNA-1
chr1_4 CDS 27701920 27702015 27701920 ID=Msa0158430-mRNA-1:cds;Parent=Msa0158430-mRNA-1
Gene Sequence

>Msa0158430

ATGTTAGCGATATCTCCTCCTGTGTTTTCAACAATTGGATGGCCCTTTGAGGAGCCAATATCCCATAACCAGCATCAGAGTTCATTCTACAAAGACACTGCTGATCAATTATTTAATTTTCATGATCAAGTTGAGGCAGAAATTAATTCAAAAGATCCCTCACAATCCACAAGCAGTGACCTTGGCATGGTTAAGAAGCTTGTCCATAATGCTAGTGAACGCGATCGCCGCAAGAAGATCAATAATTTGTATTCATCACTTCATTCACTTCTTCCTGTTTCAGATCAAATGAAGAAATTGAGCATTCCAGCAACAATTTCAAGAGTCCAAAAATACATACCAGAATTACAGAAACAGGTGGAAGGACTAGTTAAGAGAAAAGAAGAGGTCTTATTGAGACTTTCTCCCCAAGTAGAAGAGGTTATTCTAAGCAAAGAATCCCAAAGGAAGAAGCATAGTTACAACTCTGGTTTTGTAGTTTCAAGTAGTAGGCTCAATGATAGTGAAATTACCATTCAGATATCATGTTACACTGTACACAAGATTCCACTTTCTGAGATCTTGATATGTTTGGAAAATGATGGCCTTTTGCTTCTTAATCTTTCTTCATCTCAGACCTTTGGAGGGAGGGTCTTCTATAATTTGCATTTCCAGGTAGATAAAACACAAAGATTAGAATCCGAAATTCTAAATGAGAAGCTCTTATCAATAATGGATAAGGAAGGGATATTTGAAGTCAACAATGATTAG
Protein sequence

>Msa0158430

MLAISPPVFSTIGWPFEEPISHNQHQSSFYKDTADQLFNFHDQVEAEINSKDPSQSTSSDLGMVKKLVHNASERDRRKKINNLYSSLHSLLPVSDQMKKLSIPATISRVQKYIPELQKQVEGLVKRKEEVLLRLSPQVEEVILSKESQRKKHSYNSGFVVSSSRLNDSEITIQISCYTVHKIPLSEILICLENDGLLLLNLSSSQTFGGRVFYNLHFQVDKTQRLESEILNEKLLSIMDKEGIFEVNND*