AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0158440


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0158440 MtrunA17_Chr1g0170581 92.308 247 19 0 1 247 1 247 1.94e-166 459
Msa0158440 MtrunA17_Chr1g0170571 88.115 244 21 1 1 244 1 236 2.87e-146 408
Msa0158440 MtrunA17_Chr1g0170591 82.787 244 34 1 1 244 1 236 3.53e-145 405
Msa0158440 MtrunA17_Chr7g0256671 56.574 251 90 7 1 241 1 242 1.05e-63 199
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0158440 AT3G56970.1 41.767 249 134 5 1 240 1 247 4.16e-56 180
Msa0158440 AT3G56980.1 39.560 273 115 6 1 242 1 254 2.35e-47 158
Msa0158440 AT2G41240.1 38.400 250 134 5 1 242 1 238 5.70e-46 154
Msa0158440 AT2G41240.2 38.000 250 134 6 1 242 1 237 1.89e-43 147
Msa0158440 AT5G04150.2 45.806 155 82 1 65 219 86 238 2.64e-36 129
Msa0158440 AT5G04150.1 44.000 175 93 3 65 237 86 257 4.34e-36 129

Find 34 sgRNAs with CRISPR-Local

Find 118 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACTTGTTTCTGTAATTC+TGG 0.201065 1_4:+27720531 None:intergenic
TCTTCATCTCAGACCTTTGC+AGG 0.241758 1_4:-27720267 Msa0158440:CDS
TGTAACTATGCTTCTTCCTT+TGG 0.251062 1_4:+27720430 None:intergenic
CTTCTATAATTTGCATTTCC+AGG 0.265122 1_4:-27720238 Msa0158440:intron
GTAACTATGCTTCTTCCTTT+GGG 0.307422 1_4:+27720431 None:intergenic
AGGACTCTTGAAATTGTTGC+TGG 0.367920 1_4:+27720564 None:intergenic
GATCGCGTTCACTAGCATTA+TGG 0.375108 1_4:+27720874 None:intergenic
TCTTCCTGTTTCTGATCAAA+TGG 0.413993 1_4:-27720808 Msa0158440:intron
AGTACCATTTGATCAGAAAC+AGG 0.414053 1_4:+27720804 None:intergenic
ATGAATTCTGATGATGGTTA+TGG 0.420513 1_4:+27721015 None:intergenic
AACAATTGGATGGCCCTTTG+AGG 0.464251 1_4:-27721048 Msa0158440:CDS
TGAAGGACTAAGTAAGAGAA+AGG 0.469978 1_4:-27720511 Msa0158440:CDS
AGAATAACCTCTTCTTCTTG+AGG 0.516991 1_4:+27720465 None:intergenic
TGTAGAATGAATTCTGATGA+TGG 0.522101 1_4:+27721009 None:intergenic
CATGCTAAGGTCACTGCTTG+TGG 0.529098 1_4:+27720911 None:intergenic
ACAAATTATTGATCTTCTTG+CGG 0.529574 1_4:+27720850 None:intergenic
CTTCATCTCAGACCTTTGCA+GGG 0.548412 1_4:-27720266 Msa0158440:CDS
TCTCAGAAAGTGAAATCTTG+TGG 0.550047 1_4:+27720334 None:intergenic
AAGAAGCATAGTTACAACTC+TGG 0.551749 1_4:-27720423 Msa0158440:CDS
ACTTTATCCTCAAGAAGAAG+AGG 0.554683 1_4:-27720472 Msa0158440:CDS
AGTGTAACATGAAATCTGAA+TGG 0.555088 1_4:+27720359 None:intergenic
GAATTACAGAAACAAGTTGA+AGG 0.556198 1_4:-27720528 Msa0158440:CDS
CATCCAATTGTTGAAAACAT+AGG 0.558093 1_4:+27721059 None:intergenic
AGGAAGAAGTGAACGAAGAG+AGG 0.567551 1_4:+27720824 None:intergenic
GCTCTTATCAATAATCGAGA+AGG 0.576892 1_4:-27719665 Msa0158440:CDS
TTGATGTGTTTAGAAAATGA+TGG 0.577722 1_4:-27720309 Msa0158440:CDS
ATAGAAGACTCTCCCTGCAA+AGG 0.579465 1_4:+27720254 None:intergenic
CCAATTGTTGAAAACATAGG+AGG 0.582074 1_4:+27721062 None:intergenic
GTCACTGCTTGTGGATTGTG+AGG 0.596424 1_4:+27720920 None:intergenic
CAAGCTTCTTGACCATGCTA+AGG 0.604223 1_4:+27720898 None:intergenic
CACAAGCAGTGACCTTAGCA+TGG 0.613188 1_4:-27720910 Msa0158440:CDS
TTCTAAGCAAAGAATCCCAA+AGG 0.620328 1_4:-27720446 Msa0158440:CDS
TTATCAATAATCGAGAAGGA+AGG 0.636620 1_4:-27719661 Msa0158440:CDS
TCACTGCTTGTGGATTGTGA+GGG 0.698792 1_4:+27720921 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTCACAAATGTTAAATT+TGG - chr1_4:27720730-27720749 Msa0158440:intron 15.0%
!! ATACAAAAAAATTGATTACT+TGG - chr1_4:27720875-27720894 Msa0158440:CDS 15.0%
!! TACAAAAAAATTGATTACTT+GGG - chr1_4:27720876-27720895 Msa0158440:CDS 15.0%
!!! ATTTTCACAAATGTTAAATT+TGG - chr1_4:27720730-27720749 Msa0158440:intron 15.0%
!! ATACAAAAAAATTGATTACT+TGG - chr1_4:27720875-27720894 Msa0158440:CDS 15.0%
!! TACAAAAAAATTGATTACTT+GGG - chr1_4:27720876-27720895 Msa0158440:CDS 15.0%
!! GATCATACATAATTATGATT+TGG - chr1_4:27720651-27720670 Msa0158440:intron 20.0%
!! TTAAAAACATGTAAGAAGTA+AGG - chr1_4:27720813-27720832 Msa0158440:CDS 20.0%
!! GATCATACATAATTATGATT+TGG - chr1_4:27720651-27720670 Msa0158440:intron 20.0%
!! TTAAAAACATGTAAGAAGTA+AGG - chr1_4:27720813-27720832 Msa0158440:CDS 20.0%
!! TAATTTTCATGATCAAGTAG+AGG - chr1_4:27719755-27719774 Msa0158440:intron 25.0%
! ACAAATTATTGATCTTCTTG+CGG + chr1_4:27719872-27719891 None:intergenic 25.0%
! ACAATTAGAAACAGATAGAA+TGG + chr1_4:27719949-27719968 None:intergenic 25.0%
! TCTAATTGTCAATAATGCTT+TGG - chr1_4:27719960-27719979 Msa0158440:intron 25.0%
!! GTAATTCTGGTATGTATTTT+AGG + chr1_4:27720178-27720197 None:intergenic 25.0%
! TTGATGTGTTTAGAAAATGA+TGG - chr1_4:27720410-27720429 Msa0158440:CDS 25.0%
!!! ACACAAAGAGCTTTTAATTA+AGG + chr1_4:27720981-27721000 None:intergenic 25.0%
!! TAATTTTCATGATCAAGTAG+AGG - chr1_4:27719755-27719774 Msa0158440:intron 25.0%
! ACAAATTATTGATCTTCTTG+CGG + chr1_4:27719872-27719891 None:intergenic 25.0%
! ACAATTAGAAACAGATAGAA+TGG + chr1_4:27719949-27719968 None:intergenic 25.0%
! TCTAATTGTCAATAATGCTT+TGG - chr1_4:27719960-27719979 Msa0158440:intron 25.0%
!! GTAATTCTGGTATGTATTTT+AGG + chr1_4:27720178-27720197 None:intergenic 25.0%
! TTGATGTGTTTAGAAAATGA+TGG - chr1_4:27720410-27720429 Msa0158440:CDS 25.0%
!!! ACACAAAGAGCTTTTAATTA+AGG + chr1_4:27720981-27721000 None:intergenic 25.0%
CATCCAATTGTTGAAAACAT+AGG + chr1_4:27719663-27719682 None:intergenic 30.0%
! CTATGTTTTCAACAATTGGA+TGG - chr1_4:27719661-27719680 Msa0158440:CDS 30.0%
!! ATGAATTCTGATGATGGTTA+TGG + chr1_4:27719707-27719726 None:intergenic 30.0%
TGTAGAATGAATTCTGATGA+TGG + chr1_4:27719713-27719732 None:intergenic 30.0%
!!! TCTAATTGTGATGTCGTTTT+TGG - chr1_4:27720056-27720075 Msa0158440:intron 30.0%
!! TCAACTTGTTTCTGTAATTC+TGG + chr1_4:27720191-27720210 None:intergenic 30.0%
! GAATTACAGAAACAAGTTGA+AGG - chr1_4:27720191-27720210 Msa0158440:intron 30.0%
!! GTTTTGTAGTTTCAAGTAGT+AGG - chr1_4:27720318-27720337 Msa0158440:CDS 30.0%
AGTGTAACATGAAATCTGAA+TGG + chr1_4:27720363-27720382 None:intergenic 30.0%
! CTTCTATAATTTGCATTTCC+AGG - chr1_4:27720481-27720500 Msa0158440:CDS 30.0%
GATGTATAGACTCATAAAAG+AGG - chr1_4:27720940-27720959 Msa0158440:CDS 30.0%
TTATCAATAATCGAGAAGGA+AGG - chr1_4:27721058-27721077 Msa0158440:CDS 30.0%
CATCCAATTGTTGAAAACAT+AGG + chr1_4:27719663-27719682 None:intergenic 30.0%
! CTATGTTTTCAACAATTGGA+TGG - chr1_4:27719661-27719680 Msa0158440:CDS 30.0%
!! ATGAATTCTGATGATGGTTA+TGG + chr1_4:27719707-27719726 None:intergenic 30.0%
TGTAGAATGAATTCTGATGA+TGG + chr1_4:27719713-27719732 None:intergenic 30.0%
!!! TCTAATTGTGATGTCGTTTT+TGG - chr1_4:27720056-27720075 Msa0158440:intron 30.0%
!! TCAACTTGTTTCTGTAATTC+TGG + chr1_4:27720191-27720210 None:intergenic 30.0%
! GAATTACAGAAACAAGTTGA+AGG - chr1_4:27720191-27720210 Msa0158440:intron 30.0%
!! GTTTTGTAGTTTCAAGTAGT+AGG - chr1_4:27720318-27720337 Msa0158440:CDS 30.0%
AGTGTAACATGAAATCTGAA+TGG + chr1_4:27720363-27720382 None:intergenic 30.0%
! CTTCTATAATTTGCATTTCC+AGG - chr1_4:27720481-27720500 Msa0158440:CDS 30.0%
GATGTATAGACTCATAAAAG+AGG - chr1_4:27720940-27720959 Msa0158440:CDS 30.0%
TTATCAATAATCGAGAAGGA+AGG - chr1_4:27721058-27721077 Msa0158440:CDS 30.0%
CCAATTGTTGAAAACATAGG+AGG + chr1_4:27719660-27719679 None:intergenic 35.0%
! CCTCCTATGTTTTCAACAAT+TGG - chr1_4:27719657-27719676 Msa0158440:CDS 35.0%
!!! TGATGATGGTTATGGCTTTT+TGG + chr1_4:27719699-27719718 None:intergenic 35.0%
!! TCTTCCTGTTTCTGATCAAA+TGG - chr1_4:27719911-27719930 Msa0158440:intron 35.0%
AGTACCATTTGATCAGAAAC+AGG + chr1_4:27719918-27719937 None:intergenic 35.0%
! CTTGAAAACCTTGTGTTTTG+AGG - chr1_4:27720017-27720036 Msa0158440:intron 35.0%
TGAAGGACTAAGTAAGAGAA+AGG - chr1_4:27720208-27720227 Msa0158440:intron 35.0%
ACTTTATCCTCAAGAAGAAG+AGG - chr1_4:27720247-27720266 Msa0158440:CDS 35.0%
AGAATAACCTCTTCTTCTTG+AGG + chr1_4:27720257-27720276 None:intergenic 35.0%
TTCTAAGCAAAGAATCCCAA+AGG - chr1_4:27720273-27720292 Msa0158440:CDS 35.0%
GTAACTATGCTTCTTCCTTT+GGG + chr1_4:27720291-27720310 None:intergenic 35.0%
TGTAACTATGCTTCTTCCTT+TGG + chr1_4:27720292-27720311 None:intergenic 35.0%
! AAGAAGCATAGTTACAACTC+TGG - chr1_4:27720296-27720315 Msa0158440:CDS 35.0%
TCTCAGAAAGTGAAATCTTG+TGG + chr1_4:27720388-27720407 None:intergenic 35.0%
GCTCTTATCAATAATCGAGA+AGG - chr1_4:27721054-27721073 Msa0158440:CDS 35.0%
CCAATTGTTGAAAACATAGG+AGG + chr1_4:27719660-27719679 None:intergenic 35.0%
! CCTCCTATGTTTTCAACAAT+TGG - chr1_4:27719657-27719676 Msa0158440:CDS 35.0%
!!! TGATGATGGTTATGGCTTTT+TGG + chr1_4:27719699-27719718 None:intergenic 35.0%
!! TCTTCCTGTTTCTGATCAAA+TGG - chr1_4:27719911-27719930 Msa0158440:intron 35.0%
AGTACCATTTGATCAGAAAC+AGG + chr1_4:27719918-27719937 None:intergenic 35.0%
! CTTGAAAACCTTGTGTTTTG+AGG - chr1_4:27720017-27720036 Msa0158440:intron 35.0%
TGAAGGACTAAGTAAGAGAA+AGG - chr1_4:27720208-27720227 Msa0158440:intron 35.0%
ACTTTATCCTCAAGAAGAAG+AGG - chr1_4:27720247-27720266 Msa0158440:CDS 35.0%
AGAATAACCTCTTCTTCTTG+AGG + chr1_4:27720257-27720276 None:intergenic 35.0%
TTCTAAGCAAAGAATCCCAA+AGG - chr1_4:27720273-27720292 Msa0158440:CDS 35.0%
GTAACTATGCTTCTTCCTTT+GGG + chr1_4:27720291-27720310 None:intergenic 35.0%
TGTAACTATGCTTCTTCCTT+TGG + chr1_4:27720292-27720311 None:intergenic 35.0%
! AAGAAGCATAGTTACAACTC+TGG - chr1_4:27720296-27720315 Msa0158440:CDS 35.0%
TCTCAGAAAGTGAAATCTTG+TGG + chr1_4:27720388-27720407 None:intergenic 35.0%
GCTCTTATCAATAATCGAGA+AGG - chr1_4:27721054-27721073 Msa0158440:CDS 35.0%
!!! ACCTTGTGTTTTGAGGAGTA+TGG - chr1_4:27720024-27720043 Msa0158440:intron 40.0%
ACCATACTCCTCAAAACACA+AGG + chr1_4:27720028-27720047 None:intergenic 40.0%
AGGACTCTTGAAATTGTTGC+TGG + chr1_4:27720158-27720177 None:intergenic 40.0%
CCTAGTGAAATTAACACACC+TGG + chr1_4:27720502-27720521 None:intergenic 40.0%
CCAGGTGTGTTAATTTCACT+AGG - chr1_4:27720499-27720518 Msa0158440:CDS 40.0%
! AAGCTCTTTGTGTTTTCTGC+AGG - chr1_4:27720988-27721007 Msa0158440:CDS 40.0%
!!! ACCTTGTGTTTTGAGGAGTA+TGG - chr1_4:27720024-27720043 Msa0158440:intron 40.0%
ACCATACTCCTCAAAACACA+AGG + chr1_4:27720028-27720047 None:intergenic 40.0%
AGGACTCTTGAAATTGTTGC+TGG + chr1_4:27720158-27720177 None:intergenic 40.0%
CCTAGTGAAATTAACACACC+TGG + chr1_4:27720502-27720521 None:intergenic 40.0%
CCAGGTGTGTTAATTTCACT+AGG - chr1_4:27720499-27720518 Msa0158440:CDS 40.0%
! AAGCTCTTTGTGTTTTCTGC+AGG - chr1_4:27720988-27721007 Msa0158440:CDS 40.0%
AACAATTGGATGGCCCTTTG+AGG - chr1_4:27719671-27719690 Msa0158440:CDS 45.0%
!!! TGGCTTTTTGGCTCCTCAAA+GGG + chr1_4:27719687-27719706 None:intergenic 45.0%
!!! ATGGCTTTTTGGCTCCTCAA+AGG + chr1_4:27719688-27719707 None:intergenic 45.0%
! TCACTGCTTGTGGATTGTGA+GGG + chr1_4:27719801-27719820 None:intergenic 45.0%
CAAGCTTCTTGACCATGCTA+AGG + chr1_4:27719824-27719843 None:intergenic 45.0%
GATCGCGTTCACTAGCATTA+TGG + chr1_4:27719848-27719867 None:intergenic 45.0%
AGGAAGAAGTGAACGAAGAG+AGG + chr1_4:27719898-27719917 None:intergenic 45.0%
TCTTCATCTCAGACCTTTGC+AGG - chr1_4:27720452-27720471 Msa0158440:CDS 45.0%
CTTCATCTCAGACCTTTGCA+GGG - chr1_4:27720453-27720472 Msa0158440:CDS 45.0%
ATAGAAGACTCTCCCTGCAA+AGG + chr1_4:27720468-27720487 None:intergenic 45.0%
! CTCTTTGTGTTTTCTGCAGG+TGG - chr1_4:27720991-27721010 Msa0158440:CDS 45.0%
AACAATTGGATGGCCCTTTG+AGG - chr1_4:27719671-27719690 Msa0158440:CDS 45.0%
!!! TGGCTTTTTGGCTCCTCAAA+GGG + chr1_4:27719687-27719706 None:intergenic 45.0%
!!! ATGGCTTTTTGGCTCCTCAA+AGG + chr1_4:27719688-27719707 None:intergenic 45.0%
! TCACTGCTTGTGGATTGTGA+GGG + chr1_4:27719801-27719820 None:intergenic 45.0%
CAAGCTTCTTGACCATGCTA+AGG + chr1_4:27719824-27719843 None:intergenic 45.0%
GATCGCGTTCACTAGCATTA+TGG + chr1_4:27719848-27719867 None:intergenic 45.0%
AGGAAGAAGTGAACGAAGAG+AGG + chr1_4:27719898-27719917 None:intergenic 45.0%
TCTTCATCTCAGACCTTTGC+AGG - chr1_4:27720452-27720471 Msa0158440:CDS 45.0%
CTTCATCTCAGACCTTTGCA+GGG - chr1_4:27720453-27720472 Msa0158440:CDS 45.0%
ATAGAAGACTCTCCCTGCAA+AGG + chr1_4:27720468-27720487 None:intergenic 45.0%
! CTCTTTGTGTTTTCTGCAGG+TGG - chr1_4:27720991-27721010 Msa0158440:CDS 45.0%
! GTCACTGCTTGTGGATTGTG+AGG + chr1_4:27719802-27719821 None:intergenic 50.0%
CATGCTAAGGTCACTGCTTG+TGG + chr1_4:27719811-27719830 None:intergenic 50.0%
CACAAGCAGTGACCTTAGCA+TGG - chr1_4:27719809-27719828 Msa0158440:intron 50.0%
! GTCACTGCTTGTGGATTGTG+AGG + chr1_4:27719802-27719821 None:intergenic 50.0%
CATGCTAAGGTCACTGCTTG+TGG + chr1_4:27719811-27719830 None:intergenic 50.0%
CACAAGCAGTGACCTTAGCA+TGG - chr1_4:27719809-27719828 Msa0158440:intron 50.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 27719642 27721099 27719642 ID=Msa0158440;Name=Msa0158440
chr1_4 mRNA 27719642 27721099 27719642 ID=Msa0158440-mRNA-1;Parent=Msa0158440;Name=Msa0158440-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0|0|1|1|1|3|0|247
chr1_4 exon 27720809 27721099 27720809 ID=Msa0158440-mRNA-1:exon:9031;Parent=Msa0158440-mRNA-1
chr1_4 exon 27720239 27720601 27720239 ID=Msa0158440-mRNA-1:exon:9030;Parent=Msa0158440-mRNA-1
chr1_4 exon 27719642 27719731 27719642 ID=Msa0158440-mRNA-1:exon:9029;Parent=Msa0158440-mRNA-1
chr1_4 CDS 27720809 27721099 27720809 ID=Msa0158440-mRNA-1:cds;Parent=Msa0158440-mRNA-1
chr1_4 CDS 27720239 27720601 27720239 ID=Msa0158440-mRNA-1:cds;Parent=Msa0158440-mRNA-1
chr1_4 CDS 27719642 27719731 27719642 ID=Msa0158440-mRNA-1:cds;Parent=Msa0158440-mRNA-1
Gene Sequence

>Msa0158440

ATGTTAGCGATATCTCCTCCTATGTTTTCAACAATTGGATGGCCCTTTGAGGAGCCAAAAAGCCATAACCATCATCAGAATTCATTCTACAAAGACACTGTTGATCAATTATTTAATTTTCATGATCAAGTAGAGGCAGAAATTAATTCAACAGATCCCTCACAATCCACAAGCAGTGACCTTAGCATGGTCAAGAAGCTTGTCCATAATGCTAGTGAACGCGATCGCCGCAAGAAGATCAATAATTTGTATTCCTCTCTTCGTTCACTTCTTCCTGTTTCTGATCAAATGAAGAAATTGAGCATTCCAGCAACAATTTCAAGAGTCCTAAAATACATACCAGAATTACAGAAACAAGTTGAAGGACTAAGTAAGAGAAAGGATGAGATCTTATTGAGACTTTATCCTCAAGAAGAAGAGGTTATTCTAAGCAAAGAATCCCAAAGGAAGAAGCATAGTTACAACTCTGGTTTTGTAGTTTCAAGTAGTAGGCTCAATGATAGTGAAATTACCATTCAGATTTCATGTTACACTGTCCACAAGATTTCACTTTCTGAGATCTTGATGTGTTTAGAAAATGATGGTTTTTTGCTTCTTAATCTTTCTTCATCTCAGACCTTTGCAGGGAGAGTCTTCTATAATTTGCATTTCCAGGTGGATAAAGCACAGAGATTAGAATCTCATATTCTAAATGAGAAGCTCTTATCAATAATCGAGAAGGAAGGAGAGTTTTTAAAACAATAA

Protein sequence

>Msa0158440

MLAISPPMFSTIGWPFEEPKSHNHHQNSFYKDTVDQLFNFHDQVEAEINSTDPSQSTSSDLSMVKKLVHNASERDRRKKINNLYSSLRSLLPVSDQMKKLSIPATISRVLKYIPELQKQVEGLSKRKDEILLRLYPQEEEVILSKESQRKKHSYNSGFVVSSSRLNDSEITIQISCYTVHKISLSEILMCLENDGFLLLNLSSSQTFAGRVFYNLHFQVDKAQRLESHILNEKLLSIIEKEGEFLKQ*