Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0158770 | KAG4935703.1 | 77.083 | 96 | 12 | 2 | 1 | 88 | 1 | 94 | 1.03e-41 | 145 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0158770 | sp|P53681|CRK_DAUCA | 65.517 | 116 | 23 | 2 | 1 | 110 | 187 | 291 | 1.78e-39 | 143 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0158770 | A0A0B2RD77 | 76.042 | 96 | 13 | 2 | 1 | 88 | 1 | 94 | 2.11e-41 | 144 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0158770 | MtrunA17_Chr4g0045261 | 81.250 | 96 | 8 | 2 | 1 | 88 | 176 | 269 | 9.49e-44 | 152 |
| Msa0158770 | MtrunA17_Chr8g0374701 | 72.340 | 94 | 20 | 1 | 1 | 88 | 167 | 260 | 7.80e-39 | 138 |
| Msa0158770 | MtrunA17_Chr5g0402551 | 69.149 | 94 | 23 | 1 | 1 | 88 | 173 | 266 | 7.79e-38 | 135 |
| Msa0158770 | MtrunA17_Chr7g0256001 | 67.021 | 94 | 25 | 1 | 1 | 88 | 180 | 273 | 1.77e-36 | 132 |
| Msa0158770 | MtrunA17_Chr3g0127571 | 68.085 | 94 | 24 | 1 | 1 | 88 | 177 | 270 | 5.06e-36 | 130 |
| Msa0158770 | MtrunA17_Chr7g0276111 | 62.766 | 94 | 29 | 1 | 1 | 88 | 183 | 276 | 6.33e-33 | 122 |
| Msa0158770 | MtrunA17_Chr5g0405421 | 45.600 | 125 | 46 | 4 | 1 | 116 | 135 | 246 | 8.94e-23 | 93.6 |
| Msa0158770 | MtrunA17_Chr1g0157131 | 48.276 | 87 | 40 | 1 | 7 | 88 | 116 | 202 | 2.84e-19 | 83.6 |
| Msa0158770 | MtrunA17_Chr1g0173541 | 45.161 | 93 | 46 | 1 | 1 | 88 | 88 | 180 | 2.68e-17 | 78.2 |
| Msa0158770 | MtrunA17_Chr5g0444181 | 40.698 | 86 | 46 | 1 | 8 | 88 | 134 | 219 | 3.82e-17 | 75.9 |
| Msa0158770 | MtrunA17_Chr7g0257751 | 45.161 | 93 | 46 | 1 | 1 | 88 | 94 | 186 | 5.32e-17 | 77.0 |
| Msa0158770 | MtrunA17_Chr4g0033371 | 44.086 | 93 | 47 | 1 | 1 | 88 | 108 | 200 | 4.06e-16 | 74.7 |
| Msa0158770 | MtrunA17_Chr5g0444171 | 40.698 | 86 | 46 | 1 | 8 | 88 | 135 | 220 | 6.12e-16 | 73.9 |
| Msa0158770 | MtrunA17_Chr4g0059951 | 43.011 | 93 | 48 | 1 | 1 | 88 | 109 | 201 | 1.63e-15 | 72.8 |
| Msa0158770 | MtrunA17_Chr3g0098951 | 40.698 | 86 | 46 | 1 | 8 | 88 | 127 | 212 | 1.80e-15 | 72.8 |
| Msa0158770 | MtrunA17_Chr5g0441691 | 34.921 | 126 | 66 | 2 | 7 | 127 | 110 | 224 | 2.25e-14 | 69.7 |
| Msa0158770 | MtrunA17_Chr1g0176421 | 39.785 | 93 | 51 | 1 | 1 | 88 | 197 | 289 | 1.30e-13 | 67.4 |
| Msa0158770 | MtrunA17_Chr4g0074811 | 39.535 | 86 | 47 | 1 | 8 | 88 | 134 | 219 | 1.39e-13 | 67.4 |
| Msa0158770 | MtrunA17_Chr8g0387671 | 41.304 | 92 | 49 | 1 | 7 | 93 | 108 | 199 | 2.36e-13 | 66.6 |
| Msa0158770 | MtrunA17_Chr7g0242561 | 39.785 | 93 | 51 | 1 | 1 | 88 | 92 | 184 | 5.76e-13 | 65.5 |
| Msa0158770 | MtrunA17_Chr0c02g0489761 | 39.080 | 87 | 48 | 1 | 7 | 88 | 120 | 206 | 9.60e-13 | 65.1 |
| Msa0158770 | MtrunA17_Chr1g0170281 | 37.634 | 93 | 53 | 1 | 1 | 88 | 154 | 246 | 1.74e-12 | 64.3 |
| Msa0158770 | MtrunA17_Chr7g0267771 | 41.379 | 87 | 46 | 1 | 7 | 88 | 130 | 216 | 1.85e-12 | 63.9 |
| Msa0158770 | MtrunA17_Chr6g0453681 | 38.372 | 86 | 47 | 2 | 8 | 88 | 62 | 146 | 3.11e-12 | 63.5 |
| Msa0158770 | MtrunA17_Chr6g0468751 | 38.372 | 86 | 47 | 2 | 8 | 88 | 62 | 146 | 3.22e-12 | 63.5 |
| Msa0158770 | MtrunA17_Chr5g0448721 | 41.379 | 87 | 46 | 1 | 7 | 88 | 138 | 224 | 3.51e-12 | 63.2 |
| Msa0158770 | MtrunA17_Chr1g0203881 | 29.032 | 93 | 61 | 1 | 1 | 88 | 77 | 169 | 9.14e-12 | 62.0 |
| Msa0158770 | MtrunA17_Chr7g0267751 | 41.304 | 92 | 39 | 2 | 7 | 88 | 177 | 263 | 2.70e-11 | 60.8 |
| Msa0158770 | MtrunA17_Chr4g0074791 | 36.782 | 87 | 49 | 1 | 8 | 88 | 138 | 224 | 4.05e-11 | 60.1 |
| Msa0158770 | MtrunA17_Chr3g0132591 | 34.884 | 86 | 51 | 1 | 8 | 88 | 148 | 233 | 5.18e-11 | 59.7 |
| Msa0158770 | MtrunA17_Chr8g0385341 | 34.211 | 114 | 59 | 2 | 8 | 116 | 73 | 175 | 6.83e-11 | 59.7 |
| Msa0158770 | MtrunA17_Chr1g0176751 | 35.484 | 93 | 55 | 1 | 1 | 88 | 160 | 252 | 8.56e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0158770 | AT3G19100.1 | 72.340 | 94 | 20 | 1 | 1 | 88 | 183 | 276 | 1.19e-38 | 138 |
| Msa0158770 | AT3G50530.1 | 72.340 | 94 | 20 | 1 | 1 | 88 | 187 | 280 | 1.29e-38 | 138 |
| Msa0158770 | AT3G50530.2 | 72.340 | 94 | 20 | 1 | 1 | 88 | 187 | 280 | 1.86e-38 | 138 |
| Msa0158770 | AT2G41140.1 | 72.340 | 94 | 20 | 1 | 1 | 88 | 162 | 255 | 2.34e-37 | 134 |
| Msa0158770 | AT3G56760.1 | 72.340 | 94 | 20 | 1 | 1 | 88 | 163 | 256 | 4.36e-37 | 134 |
| Msa0158770 | AT1G49580.1 | 69.149 | 94 | 23 | 1 | 1 | 88 | 189 | 282 | 3.78e-36 | 131 |
| Msa0158770 | AT2G46700.2 | 65.263 | 95 | 27 | 1 | 1 | 89 | 1 | 95 | 2.91e-34 | 124 |
| Msa0158770 | AT2G46700.3 | 65.263 | 95 | 27 | 1 | 1 | 89 | 1 | 95 | 2.91e-34 | 124 |
| Msa0158770 | AT2G46700.1 | 65.957 | 94 | 26 | 1 | 1 | 88 | 182 | 275 | 9.37e-34 | 125 |
| Msa0158770 | AT3G49370.1 | 56.383 | 94 | 35 | 1 | 1 | 88 | 181 | 274 | 2.64e-29 | 112 |
| Msa0158770 | AT5G24430.1 | 55.319 | 94 | 36 | 1 | 1 | 88 | 182 | 275 | 2.32e-28 | 110 |
| Msa0158770 | AT2G17890.1 | 48.000 | 125 | 43 | 4 | 1 | 116 | 144 | 255 | 1.34e-25 | 102 |
| Msa0158770 | AT4G36070.1 | 44.000 | 125 | 48 | 3 | 1 | 116 | 107 | 218 | 8.52e-23 | 94.0 |
| Msa0158770 | AT4G36070.2 | 44.000 | 125 | 48 | 3 | 1 | 116 | 107 | 218 | 1.21e-22 | 93.6 |
| Msa0158770 | AT5G66210.6 | 49.485 | 97 | 38 | 2 | 1 | 88 | 98 | 192 | 7.99e-22 | 90.9 |
| Msa0158770 | AT5G66210.4 | 49.485 | 97 | 38 | 2 | 1 | 88 | 98 | 192 | 7.99e-22 | 90.9 |
| Msa0158770 | AT5G66210.1 | 49.485 | 97 | 38 | 2 | 1 | 88 | 98 | 192 | 1.04e-21 | 90.9 |
| Msa0158770 | AT5G66210.2 | 49.485 | 97 | 38 | 2 | 1 | 88 | 98 | 192 | 1.04e-21 | 90.9 |
| Msa0158770 | AT5G66210.5 | 49.485 | 97 | 38 | 2 | 1 | 88 | 98 | 192 | 1.18e-21 | 90.9 |
| Msa0158770 | AT5G66210.3 | 49.485 | 97 | 38 | 2 | 1 | 88 | 98 | 192 | 1.19e-21 | 90.9 |
| Msa0158770 | AT1G76040.3 | 48.276 | 87 | 40 | 1 | 7 | 88 | 154 | 240 | 1.39e-18 | 82.0 |
| Msa0158770 | AT1G76040.2 | 48.276 | 87 | 40 | 1 | 7 | 88 | 154 | 240 | 1.59e-18 | 82.0 |
| Msa0158770 | AT2G31500.1 | 46.237 | 93 | 45 | 1 | 1 | 88 | 102 | 194 | 3.37e-18 | 80.9 |
| Msa0158770 | AT5G19450.2 | 47.312 | 93 | 44 | 1 | 1 | 88 | 93 | 185 | 5.27e-18 | 80.5 |
| Msa0158770 | AT5G19450.1 | 47.312 | 93 | 44 | 1 | 1 | 88 | 93 | 185 | 5.27e-18 | 80.5 |
| Msa0158770 | AT5G12480.1 | 46.237 | 93 | 45 | 1 | 1 | 88 | 95 | 187 | 7.05e-18 | 80.1 |
| Msa0158770 | AT4G04700.1 | 41.111 | 90 | 44 | 1 | 8 | 88 | 71 | 160 | 1.01e-16 | 76.6 |
| Msa0158770 | AT1G74740.1 | 43.011 | 93 | 48 | 1 | 1 | 88 | 95 | 187 | 1.02e-15 | 73.9 |
| Msa0158770 | AT4G21940.3 | 37.634 | 93 | 53 | 1 | 1 | 88 | 138 | 230 | 1.78e-15 | 73.2 |
| Msa0158770 | AT4G21940.1 | 37.634 | 93 | 53 | 1 | 1 | 88 | 138 | 230 | 2.02e-15 | 73.2 |
| Msa0158770 | AT4G21940.2 | 37.634 | 93 | 53 | 1 | 1 | 88 | 138 | 230 | 2.17e-15 | 72.8 |
| Msa0158770 | AT3G57530.2 | 41.935 | 93 | 49 | 1 | 1 | 88 | 99 | 191 | 2.18e-15 | 72.8 |
| Msa0158770 | AT1G50700.3 | 43.023 | 86 | 44 | 1 | 8 | 88 | 116 | 201 | 3.32e-15 | 72.4 |
| Msa0158770 | AT3G57530.1 | 41.935 | 93 | 49 | 1 | 1 | 88 | 99 | 191 | 3.76e-15 | 72.4 |
| Msa0158770 | AT1G50700.1 | 43.023 | 86 | 44 | 1 | 8 | 88 | 116 | 201 | 4.35e-15 | 72.0 |
| Msa0158770 | AT1G50700.2 | 43.023 | 86 | 44 | 1 | 8 | 88 | 116 | 201 | 4.36e-15 | 72.0 |
| Msa0158770 | AT3G20410.2 | 39.785 | 93 | 51 | 1 | 1 | 88 | 127 | 219 | 5.75e-15 | 71.6 |
| Msa0158770 | AT3G20410.1 | 39.785 | 93 | 51 | 1 | 1 | 88 | 127 | 219 | 5.75e-15 | 71.6 |
| Msa0158770 | AT4G04720.2 | 39.535 | 86 | 47 | 1 | 8 | 88 | 123 | 208 | 1.31e-14 | 70.9 |
| Msa0158770 | AT4G04720.1 | 39.535 | 86 | 47 | 1 | 8 | 88 | 123 | 208 | 1.31e-14 | 70.9 |
| Msa0158770 | AT2G41860.1 | 39.785 | 93 | 51 | 1 | 1 | 88 | 90 | 182 | 1.53e-14 | 70.5 |
| Msa0158770 | AT2G41860.2 | 39.785 | 93 | 51 | 1 | 1 | 88 | 90 | 182 | 1.60e-14 | 70.5 |
| Msa0158770 | AT1G18890.1 | 45.161 | 93 | 46 | 1 | 1 | 88 | 99 | 191 | 1.85e-14 | 70.1 |
| Msa0158770 | AT1G61950.1 | 39.535 | 86 | 47 | 1 | 8 | 88 | 141 | 226 | 3.39e-14 | 69.3 |
| Msa0158770 | AT5G12180.1 | 40.230 | 87 | 47 | 1 | 7 | 88 | 115 | 201 | 2.07e-13 | 67.4 |
| Msa0158770 | AT4G04695.1 | 41.111 | 90 | 44 | 2 | 8 | 88 | 71 | 160 | 3.15e-13 | 66.6 |
| Msa0158770 | AT5G19360.1 | 39.080 | 87 | 48 | 1 | 7 | 88 | 110 | 196 | 3.97e-13 | 66.2 |
| Msa0158770 | AT2G38910.1 | 37.634 | 93 | 53 | 1 | 1 | 88 | 170 | 262 | 5.91e-13 | 65.9 |
| Msa0158770 | AT4G04740.2 | 34.409 | 93 | 56 | 1 | 1 | 88 | 105 | 197 | 1.15e-12 | 65.1 |
| Msa0158770 | AT4G04740.4 | 34.409 | 93 | 56 | 1 | 1 | 88 | 105 | 197 | 1.21e-12 | 65.1 |
| Msa0158770 | AT4G04740.6 | 34.409 | 93 | 56 | 1 | 1 | 88 | 105 | 197 | 1.21e-12 | 65.1 |
| Msa0158770 | AT4G04740.5 | 34.409 | 93 | 56 | 1 | 1 | 88 | 105 | 197 | 1.21e-12 | 65.1 |
| Msa0158770 | AT4G04740.3 | 34.409 | 93 | 56 | 1 | 1 | 88 | 105 | 197 | 1.21e-12 | 65.1 |
| Msa0158770 | AT4G04740.1 | 34.409 | 93 | 56 | 1 | 1 | 88 | 105 | 197 | 1.21e-12 | 65.1 |
| Msa0158770 | AT4G04740.7 | 34.409 | 93 | 56 | 1 | 1 | 88 | 105 | 197 | 1.21e-12 | 65.1 |
| Msa0158770 | AT4G23650.1 | 36.508 | 126 | 64 | 3 | 7 | 127 | 120 | 234 | 1.70e-12 | 64.7 |
| Msa0158770 | AT5G23580.1 | 35.965 | 114 | 57 | 2 | 8 | 116 | 65 | 167 | 2.18e-12 | 64.3 |
| Msa0158770 | AT3G51850.3 | 38.710 | 93 | 52 | 1 | 1 | 88 | 90 | 182 | 2.38e-12 | 63.9 |
| Msa0158770 | AT3G51850.2 | 38.710 | 93 | 52 | 1 | 1 | 88 | 90 | 182 | 3.69e-12 | 63.5 |
| Msa0158770 | AT3G51850.1 | 38.710 | 93 | 52 | 1 | 1 | 88 | 90 | 182 | 4.18e-12 | 63.5 |
| Msa0158770 | AT5G21222.1 | 35.165 | 91 | 43 | 3 | 8 | 88 | 56 | 140 | 8.13e-12 | 62.8 |
| Msa0158770 | AT5G21222.2 | 35.165 | 91 | 43 | 3 | 8 | 88 | 56 | 140 | 8.13e-12 | 62.8 |
| Msa0158770 | AT5G21222.4 | 35.165 | 91 | 43 | 3 | 8 | 88 | 56 | 140 | 8.13e-12 | 62.8 |
| Msa0158770 | AT5G21222.3 | 35.165 | 91 | 43 | 3 | 8 | 88 | 56 | 140 | 8.13e-12 | 62.8 |
| Msa0158770 | AT5G04870.1 | 39.080 | 87 | 48 | 1 | 7 | 88 | 192 | 278 | 9.11e-12 | 62.4 |
| Msa0158770 | AT2G17290.1 | 41.379 | 87 | 46 | 1 | 7 | 88 | 127 | 213 | 1.42e-11 | 62.0 |
| Msa0158770 | AT2G17290.2 | 41.379 | 87 | 46 | 1 | 7 | 88 | 127 | 213 | 1.42e-11 | 62.0 |
| Msa0158770 | AT1G12580.1 | 31.132 | 106 | 67 | 2 | 1 | 101 | 80 | 184 | 1.42e-11 | 62.0 |
| Msa0158770 | AT3G01090.3 | 38.372 | 86 | 47 | 2 | 8 | 88 | 62 | 146 | 1.72e-11 | 61.6 |
| Msa0158770 | AT3G01090.1 | 38.372 | 86 | 47 | 2 | 8 | 88 | 62 | 146 | 1.72e-11 | 61.6 |
| Msa0158770 | AT3G10660.2 | 37.931 | 87 | 49 | 1 | 7 | 88 | 228 | 314 | 1.77e-11 | 61.6 |
| Msa0158770 | AT3G10660.1 | 37.931 | 87 | 49 | 1 | 7 | 88 | 228 | 314 | 1.77e-11 | 61.6 |
| Msa0158770 | AT3G01090.2 | 38.372 | 86 | 47 | 2 | 8 | 88 | 85 | 169 | 1.93e-11 | 61.6 |
| Msa0158770 | AT4G38230.2 | 40.230 | 87 | 47 | 1 | 7 | 88 | 96 | 182 | 2.00e-11 | 61.6 |
| Msa0158770 | AT4G38230.4 | 40.230 | 87 | 47 | 1 | 7 | 88 | 66 | 152 | 2.35e-11 | 61.2 |
| Msa0158770 | AT4G38230.3 | 40.230 | 87 | 47 | 1 | 7 | 88 | 66 | 152 | 2.35e-11 | 61.2 |
| Msa0158770 | AT4G35310.2 | 40.230 | 87 | 47 | 1 | 7 | 88 | 139 | 225 | 2.45e-11 | 61.2 |
| Msa0158770 | AT4G35310.1 | 40.230 | 87 | 47 | 1 | 7 | 88 | 139 | 225 | 2.45e-11 | 61.2 |
| Msa0158770 | AT3G29160.3 | 37.931 | 87 | 48 | 2 | 8 | 89 | 63 | 148 | 2.96e-11 | 60.8 |
| Msa0158770 | AT3G29160.1 | 37.931 | 87 | 48 | 2 | 8 | 89 | 63 | 148 | 3.43e-11 | 60.8 |
| Msa0158770 | AT3G29160.2 | 37.931 | 87 | 48 | 2 | 8 | 89 | 63 | 148 | 3.43e-11 | 60.8 |
| Msa0158770 | AT5G01810.3 | 35.294 | 85 | 50 | 2 | 8 | 88 | 55 | 138 | 5.76e-11 | 60.1 |
| Msa0158770 | AT5G01810.1 | 35.294 | 85 | 50 | 2 | 8 | 88 | 55 | 138 | 7.40e-11 | 59.7 |
| Msa0158770 | AT5G01810.2 | 35.294 | 85 | 50 | 2 | 8 | 88 | 55 | 138 | 7.40e-11 | 59.7 |
Find 26 sgRNAs with CRISPR-Local
Find 134 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGAACGGGCACAACAATT+TGG | 0.295595 | 1_4:+28482298 | Msa0158770:CDS |
| CAGCCTTGGCATCATCTTCT+AGG | 0.296079 | 1_4:-28482682 | None:intergenic |
| AACTCTTCATTTGCGACTGT+TGG | 0.374249 | 1_4:+28483324 | Msa0158770:CDS |
| TCAACAACAGACTGAAACTT+TGG | 0.406207 | 1_4:-28482895 | None:intergenic |
| ACTCTTCATTTGCGACTGTT+GGG | 0.448333 | 1_4:+28483325 | Msa0158770:CDS |
| AAATATTGCGAGCTTTGAAC+GGG | 0.456842 | 1_4:+28482285 | Msa0158770:CDS |
| GTCGCACCAACTGCCGCAAA+AGG | 0.478297 | 1_4:-28483361 | None:intergenic |
| ATGATTTATTGCTTCTTCAA+AGG | 0.493623 | 1_4:+28482650 | Msa0158770:CDS |
| ATATATCTGACTGCACAAAC+AGG | 0.495876 | 1_4:-28482957 | None:intergenic |
| AGAAAATGTCTATATTGTAA+TGG | 0.503266 | 1_4:+28482349 | Msa0158770:CDS |
| TTGGTGCGACTGTTCTATGA+AGG | 0.511935 | 1_4:+28483374 | Msa0158770:CDS |
| AAAATATTGCGAGCTTTGAA+CGG | 0.516426 | 1_4:+28482284 | Msa0158770:CDS |
| ATTTATTGCTTCTTCAAAGG+AGG | 0.543018 | 1_4:+28482653 | Msa0158770:CDS |
| TTTATTGCTTCTTCAAAGGA+GGG | 0.543699 | 1_4:+28482654 | Msa0158770:CDS |
| TATTTGTACCATGACAGCCT+TGG | 0.549299 | 1_4:-28482696 | None:intergenic |
| CAGGATCCATGCACTTCTAT+CGG | 0.551253 | 1_4:-28482938 | None:intergenic |
| AGATGATGCCAAGGCTGTCA+TGG | 0.554908 | 1_4:+28482688 | Msa0158770:CDS |
| TGTGCACCACACCCTGAAGA+TGG | 0.559527 | 1_4:-28482739 | None:intergenic |
| AAAGTTTCAGTCTGTTGTTG+AGG | 0.571170 | 1_4:+28482897 | Msa0158770:CDS |
| TGAAACTTTGGTGTAAGCAT+AGG | 0.589679 | 1_4:-28482883 | None:intergenic |
| TGAAGAAGCAATAAATCATG+TGG | 0.613601 | 1_4:-28482646 | None:intergenic |
| ATACCTAGAAGATGATGCCA+AGG | 0.618920 | 1_4:+28482679 | Msa0158770:CDS |
| GTTGAGGATAGCAGCAGATG+CGG | 0.641502 | 1_4:+28482913 | Msa0158770:CDS |
| GCGGTACCGATAGAAGTGCA+TGG | 0.643356 | 1_4:+28482932 | Msa0158770:CDS |
| GCACATAGATCTTAAGCCTG+AGG | 0.656272 | 1_4:+28482757 | Msa0158770:CDS |
| AATTGAAGATGTGAGAAGAG+AGG | 0.667853 | 1_4:+28482259 | Msa0158770:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATATAATTATTTAAAT+TGG | + | chr1_4:28483202-28483221 | Msa0158770:intron | 0.0% |
| !! | AATAATATAATTATTTAAAT+TGG | + | chr1_4:28483202-28483221 | Msa0158770:intron | 0.0% |
| !! | TAATCATATTTAAATATCTT+AGG | + | chr1_4:28482837-28482856 | Msa0158770:intron | 10.0% |
| !! | AATCATATTTAAATATCTTA+GGG | + | chr1_4:28482838-28482857 | Msa0158770:intron | 10.0% |
| !! | AAATAAAATAAAATTGAATG+GGG | - | chr1_4:28483240-28483259 | None:intergenic | 10.0% |
| !! | TAATCATATTTAAATATCTT+AGG | + | chr1_4:28482837-28482856 | Msa0158770:intron | 10.0% |
| !! | AATCATATTTAAATATCTTA+GGG | + | chr1_4:28482838-28482857 | Msa0158770:intron | 10.0% |
| !! | AAATAAAATAAAATTGAATG+GGG | - | chr1_4:28483240-28483259 | None:intergenic | 10.0% |
| !!! | ATATCACTTAATCTAATTTT+TGG | + | chr1_4:28483027-28483046 | Msa0158770:intron | 15.0% |
| !!! | ATATCACTTAATCTAATTTT+TGG | + | chr1_4:28483027-28483046 | Msa0158770:intron | 15.0% |
| !!! | TTACAATATAGACATTTTCT+TGG | - | chr1_4:28482349-28482368 | None:intergenic | 20.0% |
| !! | AGAAAATGTCTATATTGTAA+TGG | + | chr1_4:28482349-28482368 | Msa0158770:CDS | 20.0% |
| !!! | TTCCTTTTCAATTTTGATTT+TGG | + | chr1_4:28482393-28482412 | Msa0158770:intron | 20.0% |
| !!! | TTACAATATAGACATTTTCT+TGG | - | chr1_4:28482349-28482368 | None:intergenic | 20.0% |
| !! | AGAAAATGTCTATATTGTAA+TGG | + | chr1_4:28482349-28482368 | Msa0158770:CDS | 20.0% |
| !!! | TTCCTTTTCAATTTTGATTT+TGG | + | chr1_4:28482393-28482412 | Msa0158770:intron | 20.0% |
| ! | CACCAAAATCAAAATTGAAA+AGG | - | chr1_4:28482398-28482417 | None:intergenic | 25.0% |
| !!! | CTTTTCAATTTTGATTTTGG+TGG | + | chr1_4:28482396-28482415 | Msa0158770:intron | 25.0% |
| ! | TGTACATACATTTCTTAACT+AGG | - | chr1_4:28482434-28482453 | None:intergenic | 25.0% |
| !!! | TGTTTAAAAAGACTTGTTAG+AGG | - | chr1_4:28482483-28482502 | None:intergenic | 25.0% |
| ! | ATGATTTATTGCTTCTTCAA+AGG | + | chr1_4:28482650-28482669 | Msa0158770:CDS | 25.0% |
| ! | CAGATATATCTATTGTAATG+AGG | + | chr1_4:28482971-28482990 | Msa0158770:intron | 25.0% |
| ! | GTGATATTCCTAAATATTGT+TGG | - | chr1_4:28483014-28483033 | None:intergenic | 25.0% |
| ! | CACCAAAATCAAAATTGAAA+AGG | - | chr1_4:28482398-28482417 | None:intergenic | 25.0% |
| !!! | CTTTTCAATTTTGATTTTGG+TGG | + | chr1_4:28482396-28482415 | Msa0158770:intron | 25.0% |
| ! | TGTACATACATTTCTTAACT+AGG | - | chr1_4:28482434-28482453 | None:intergenic | 25.0% |
| !!! | TGTTTAAAAAGACTTGTTAG+AGG | - | chr1_4:28482483-28482502 | None:intergenic | 25.0% |
| ! | ATGATTTATTGCTTCTTCAA+AGG | + | chr1_4:28482650-28482669 | Msa0158770:CDS | 25.0% |
| ! | CAGATATATCTATTGTAATG+AGG | + | chr1_4:28482971-28482990 | Msa0158770:intron | 25.0% |
| ! | GTGATATTCCTAAATATTGT+TGG | - | chr1_4:28483014-28483033 | None:intergenic | 25.0% |
| !! | AAAATATTGCGAGCTTTGAA+CGG | + | chr1_4:28482284-28482303 | Msa0158770:CDS | 30.0% |
| ! | ATTGTAATGGAGTAAGTTTG+AGG | + | chr1_4:28482362-28482381 | Msa0158770:intron | 30.0% |
| ! | TTGTAATGGAGTAAGTTTGA+GGG | + | chr1_4:28482363-28482382 | Msa0158770:intron | 30.0% |
| AGTTTCATCAATTCAACTAG+TGG | + | chr1_4:28482534-28482553 | Msa0158770:intron | 30.0% | |
| TGAAGAAGCAATAAATCATG+TGG | - | chr1_4:28482649-28482668 | None:intergenic | 30.0% | |
| ATTTATTGCTTCTTCAAAGG+AGG | + | chr1_4:28482653-28482672 | Msa0158770:CDS | 30.0% | |
| TTTATTGCTTCTTCAAAGGA+GGG | + | chr1_4:28482654-28482673 | Msa0158770:CDS | 30.0% | |
| TCTTTCATTTGTCTTTCAGA+TGG | + | chr1_4:28482789-28482808 | Msa0158770:intron | 30.0% | |
| TATTCCTAAATATTGTTGGC+CGG | - | chr1_4:28483010-28483029 | None:intergenic | 30.0% | |
| TGAAAAACCAATACACTAGT+GGG | - | chr1_4:28483074-28483093 | None:intergenic | 30.0% | |
| TTGAAAAACCAATACACTAG+TGG | - | chr1_4:28483075-28483094 | None:intergenic | 30.0% | |
| !!! | AGTTTTCACTTGGTTTTGAA+GGG | - | chr1_4:28483308-28483327 | None:intergenic | 30.0% |
| !! | AAAATATTGCGAGCTTTGAA+CGG | + | chr1_4:28482284-28482303 | Msa0158770:CDS | 30.0% |
| ! | ATTGTAATGGAGTAAGTTTG+AGG | + | chr1_4:28482362-28482381 | Msa0158770:intron | 30.0% |
| ! | TTGTAATGGAGTAAGTTTGA+GGG | + | chr1_4:28482363-28482382 | Msa0158770:intron | 30.0% |
| AGTTTCATCAATTCAACTAG+TGG | + | chr1_4:28482534-28482553 | Msa0158770:intron | 30.0% | |
| TGAAGAAGCAATAAATCATG+TGG | - | chr1_4:28482649-28482668 | None:intergenic | 30.0% | |
| ATTTATTGCTTCTTCAAAGG+AGG | + | chr1_4:28482653-28482672 | Msa0158770:CDS | 30.0% | |
| TTTATTGCTTCTTCAAAGGA+GGG | + | chr1_4:28482654-28482673 | Msa0158770:CDS | 30.0% | |
| TCTTTCATTTGTCTTTCAGA+TGG | + | chr1_4:28482789-28482808 | Msa0158770:intron | 30.0% | |
| TATTCCTAAATATTGTTGGC+CGG | - | chr1_4:28483010-28483029 | None:intergenic | 30.0% | |
| TGAAAAACCAATACACTAGT+GGG | - | chr1_4:28483074-28483093 | None:intergenic | 30.0% | |
| TTGAAAAACCAATACACTAG+TGG | - | chr1_4:28483075-28483094 | None:intergenic | 30.0% | |
| !!! | AGTTTTCACTTGGTTTTGAA+GGG | - | chr1_4:28483308-28483327 | None:intergenic | 30.0% |
| AATTGAAGATGTGAGAAGAG+AGG | + | chr1_4:28482259-28482278 | Msa0158770:CDS | 35.0% | |
| !! | AAATATTGCGAGCTTTGAAC+GGG | + | chr1_4:28482285-28482304 | Msa0158770:CDS | 35.0% |
| !! | AAAAGACTTGTTAGAGGAAG+TGG | - | chr1_4:28482477-28482496 | None:intergenic | 35.0% |
| ! | CATCAATTCAACTAGTGGAT+TGG | + | chr1_4:28482539-28482558 | Msa0158770:intron | 35.0% |
| TAAATCATGTGGCTAGTAAG+TGG | - | chr1_4:28482638-28482657 | None:intergenic | 35.0% | |
| ! | TGAAACTTTGGTGTAAGCAT+AGG | - | chr1_4:28482886-28482905 | None:intergenic | 35.0% |
| TCAACAACAGACTGAAACTT+TGG | - | chr1_4:28482898-28482917 | None:intergenic | 35.0% | |
| AAAGTTTCAGTCTGTTGTTG+AGG | + | chr1_4:28482897-28482916 | Msa0158770:CDS | 35.0% | |
| ATATATCTGACTGCACAAAC+AGG | - | chr1_4:28482960-28482979 | None:intergenic | 35.0% | |
| ! | TTTTCCGGCCAACAATATTT+AGG | + | chr1_4:28483003-28483022 | Msa0158770:intron | 35.0% |
| ATGATTACCCACTAGTGTAT+TGG | + | chr1_4:28483064-28483083 | Msa0158770:intron | 35.0% | |
| AACATTTGCGAATGTTGTGA+AGG | + | chr1_4:28483273-28483292 | Msa0158770:intron | 35.0% | |
| ACATTTGCGAATGTTGTGAA+GGG | + | chr1_4:28483274-28483293 | Msa0158770:intron | 35.0% | |
| !!! | GTTTTCACTTGGTTTTGAAG+GGG | - | chr1_4:28483307-28483326 | None:intergenic | 35.0% |
| !!! | GAGTTTTCACTTGGTTTTGA+AGG | - | chr1_4:28483309-28483328 | None:intergenic | 35.0% |
| ! | GCAAATGAAGAGTTTTCACT+TGG | - | chr1_4:28483318-28483337 | None:intergenic | 35.0% |
| AATTGAAGATGTGAGAAGAG+AGG | + | chr1_4:28482259-28482278 | Msa0158770:CDS | 35.0% | |
| !! | AAATATTGCGAGCTTTGAAC+GGG | + | chr1_4:28482285-28482304 | Msa0158770:CDS | 35.0% |
| !! | AAAAGACTTGTTAGAGGAAG+TGG | - | chr1_4:28482477-28482496 | None:intergenic | 35.0% |
| ! | CATCAATTCAACTAGTGGAT+TGG | + | chr1_4:28482539-28482558 | Msa0158770:intron | 35.0% |
| TAAATCATGTGGCTAGTAAG+TGG | - | chr1_4:28482638-28482657 | None:intergenic | 35.0% | |
| ! | TGAAACTTTGGTGTAAGCAT+AGG | - | chr1_4:28482886-28482905 | None:intergenic | 35.0% |
| TCAACAACAGACTGAAACTT+TGG | - | chr1_4:28482898-28482917 | None:intergenic | 35.0% | |
| AAAGTTTCAGTCTGTTGTTG+AGG | + | chr1_4:28482897-28482916 | Msa0158770:CDS | 35.0% | |
| ATATATCTGACTGCACAAAC+AGG | - | chr1_4:28482960-28482979 | None:intergenic | 35.0% | |
| ! | TTTTCCGGCCAACAATATTT+AGG | + | chr1_4:28483003-28483022 | Msa0158770:intron | 35.0% |
| ATGATTACCCACTAGTGTAT+TGG | + | chr1_4:28483064-28483083 | Msa0158770:intron | 35.0% | |
| AACATTTGCGAATGTTGTGA+AGG | + | chr1_4:28483273-28483292 | Msa0158770:intron | 35.0% | |
| ACATTTGCGAATGTTGTGAA+GGG | + | chr1_4:28483274-28483293 | Msa0158770:intron | 35.0% | |
| !!! | GTTTTCACTTGGTTTTGAAG+GGG | - | chr1_4:28483307-28483326 | None:intergenic | 35.0% |
| !!! | GAGTTTTCACTTGGTTTTGA+AGG | - | chr1_4:28483309-28483328 | None:intergenic | 35.0% |
| ! | GCAAATGAAGAGTTTTCACT+TGG | - | chr1_4:28483318-28483337 | None:intergenic | 35.0% |
| TTTGAACGGGCACAACAATT+TGG | + | chr1_4:28482298-28482317 | Msa0158770:CDS | 40.0% | |
| ATACCTAGAAGATGATGCCA+AGG | + | chr1_4:28482679-28482698 | Msa0158770:CDS | 40.0% | |
| TATTTGTACCATGACAGCCT+TGG | - | chr1_4:28482699-28482718 | None:intergenic | 40.0% | |
| ! | TGTTGCATTTTGCCATCTTC+AGG | + | chr1_4:28482727-28482746 | Msa0158770:CDS | 40.0% |
| ! | GTTGCATTTTGCCATCTTCA+GGG | + | chr1_4:28482728-28482747 | Msa0158770:CDS | 40.0% |
| TGAAAGAATATGCTCACCTC+AGG | - | chr1_4:28482776-28482795 | None:intergenic | 40.0% | |
| ! | ATGAGGCTCTTCAACTTTTC+CGG | + | chr1_4:28482988-28483007 | Msa0158770:intron | 40.0% |
| AACTCTTCATTTGCGACTGT+TGG | + | chr1_4:28483324-28483343 | Msa0158770:CDS | 40.0% | |
| ACTCTTCATTTGCGACTGTT+GGG | + | chr1_4:28483325-28483344 | Msa0158770:CDS | 40.0% | |
| ! | AACTATCGCAACACCTTTTG+CGG | + | chr1_4:28483348-28483367 | Msa0158770:CDS | 40.0% |
| TTTGAACGGGCACAACAATT+TGG | + | chr1_4:28482298-28482317 | Msa0158770:CDS | 40.0% | |
| ATACCTAGAAGATGATGCCA+AGG | + | chr1_4:28482679-28482698 | Msa0158770:CDS | 40.0% | |
| TATTTGTACCATGACAGCCT+TGG | - | chr1_4:28482699-28482718 | None:intergenic | 40.0% | |
| ! | TGTTGCATTTTGCCATCTTC+AGG | + | chr1_4:28482727-28482746 | Msa0158770:CDS | 40.0% |
| ! | GTTGCATTTTGCCATCTTCA+GGG | + | chr1_4:28482728-28482747 | Msa0158770:CDS | 40.0% |
| TGAAAGAATATGCTCACCTC+AGG | - | chr1_4:28482776-28482795 | None:intergenic | 40.0% | |
| ! | ATGAGGCTCTTCAACTTTTC+CGG | + | chr1_4:28482988-28483007 | Msa0158770:intron | 40.0% |
| AACTCTTCATTTGCGACTGT+TGG | + | chr1_4:28483324-28483343 | Msa0158770:CDS | 40.0% | |
| ACTCTTCATTTGCGACTGTT+GGG | + | chr1_4:28483325-28483344 | Msa0158770:CDS | 40.0% | |
| ! | AACTATCGCAACACCTTTTG+CGG | + | chr1_4:28483348-28483367 | Msa0158770:CDS | 40.0% |
| ! | ATTTTGCCATCTTCAGGGTG+TGG | + | chr1_4:28482733-28482752 | Msa0158770:CDS | 45.0% |
| GCACATAGATCTTAAGCCTG+AGG | + | chr1_4:28482757-28482776 | Msa0158770:CDS | 45.0% | |
| CAGGATCCATGCACTTCTAT+CGG | - | chr1_4:28482941-28482960 | None:intergenic | 45.0% | |
| ! | ATTTTGCCATCTTCAGGGTG+TGG | + | chr1_4:28482733-28482752 | Msa0158770:CDS | 45.0% |
| GCACATAGATCTTAAGCCTG+AGG | + | chr1_4:28482757-28482776 | Msa0158770:CDS | 45.0% | |
| CAGGATCCATGCACTTCTAT+CGG | - | chr1_4:28482941-28482960 | None:intergenic | 45.0% | |
| !!! | AAATTTTATTATTGAAATAA+CGG | - | chr1_4:28482595-28482614 | None:intergenic | 5.0% |
| !! | AAAATAAAATAAAATTGAAT+GGG | - | chr1_4:28483241-28483260 | None:intergenic | 5.0% |
| !! | AAAAATAAAATAAAATTGAA+TGG | - | chr1_4:28483242-28483261 | None:intergenic | 5.0% |
| !!! | AAATTTTATTATTGAAATAA+CGG | - | chr1_4:28482595-28482614 | None:intergenic | 5.0% |
| !! | AAAATAAAATAAAATTGAAT+GGG | - | chr1_4:28483241-28483260 | None:intergenic | 5.0% |
| !! | AAAAATAAAATAAAATTGAA+TGG | - | chr1_4:28483242-28483261 | None:intergenic | 5.0% |
| CATGTGGCTAGTAAGTGGCA+AGG | - | chr1_4:28482633-28482652 | None:intergenic | 50.0% | |
| CAGCCTTGGCATCATCTTCT+AGG | - | chr1_4:28482685-28482704 | None:intergenic | 50.0% | |
| AGATGATGCCAAGGCTGTCA+TGG | + | chr1_4:28482688-28482707 | Msa0158770:CDS | 50.0% | |
| GTTGAGGATAGCAGCAGATG+CGG | + | chr1_4:28482913-28482932 | Msa0158770:CDS | 50.0% | |
| CATGTGGCTAGTAAGTGGCA+AGG | - | chr1_4:28482633-28482652 | None:intergenic | 50.0% | |
| CAGCCTTGGCATCATCTTCT+AGG | - | chr1_4:28482685-28482704 | None:intergenic | 50.0% | |
| AGATGATGCCAAGGCTGTCA+TGG | + | chr1_4:28482688-28482707 | Msa0158770:CDS | 50.0% | |
| GTTGAGGATAGCAGCAGATG+CGG | + | chr1_4:28482913-28482932 | Msa0158770:CDS | 50.0% | |
| TGTGCACCACACCCTGAAGA+TGG | - | chr1_4:28482742-28482761 | None:intergenic | 55.0% | |
| !! | GCGGTACCGATAGAAGTGCA+TGG | + | chr1_4:28482932-28482951 | Msa0158770:CDS | 55.0% |
| ! | GCAACACCTTTTGCGGCAGT+TGG | + | chr1_4:28483355-28483374 | Msa0158770:CDS | 55.0% |
| TGTGCACCACACCCTGAAGA+TGG | - | chr1_4:28482742-28482761 | None:intergenic | 55.0% | |
| !! | GCGGTACCGATAGAAGTGCA+TGG | + | chr1_4:28482932-28482951 | Msa0158770:CDS | 55.0% |
| ! | GCAACACCTTTTGCGGCAGT+TGG | + | chr1_4:28483355-28483374 | Msa0158770:CDS | 55.0% |
| GTCGCACCAACTGCCGCAAA+AGG | - | chr1_4:28483364-28483383 | None:intergenic | 60.0% | |
| GTCGCACCAACTGCCGCAAA+AGG | - | chr1_4:28483364-28483383 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_4 | gene | 28482242 | 28483393 | 28482242 | ID=Msa0158770;Name=Msa0158770 |
| chr1_4 | mRNA | 28482242 | 28483393 | 28482242 | ID=Msa0158770-mRNA-1;Parent=Msa0158770;Name=Msa0158770-mRNA-1;_AED=0.56;_eAED=0.73;_QI=0|0|0|0.5|1|1|4|0|162 |
| chr1_4 | exon | 28482242 | 28482372 | 28482242 | ID=Msa0158770-mRNA-1:exon:9174;Parent=Msa0158770-mRNA-1 |
| chr1_4 | exon | 28482646 | 28482778 | 28482646 | ID=Msa0158770-mRNA-1:exon:9175;Parent=Msa0158770-mRNA-1 |
| chr1_4 | exon | 28482859 | 28482984 | 28482859 | ID=Msa0158770-mRNA-1:exon:9176;Parent=Msa0158770-mRNA-1 |
| chr1_4 | exon | 28483295 | 28483393 | 28483295 | ID=Msa0158770-mRNA-1:exon:9177;Parent=Msa0158770-mRNA-1 |
| chr1_4 | CDS | 28482242 | 28482372 | 28482242 | ID=Msa0158770-mRNA-1:cds;Parent=Msa0158770-mRNA-1 |
| chr1_4 | CDS | 28482646 | 28482778 | 28482646 | ID=Msa0158770-mRNA-1:cds;Parent=Msa0158770-mRNA-1 |
| chr1_4 | CDS | 28482859 | 28482984 | 28482859 | ID=Msa0158770-mRNA-1:cds;Parent=Msa0158770-mRNA-1 |
| chr1_4 | CDS | 28483295 | 28483393 | 28483295 | ID=Msa0158770-mRNA-1:cds;Parent=Msa0158770-mRNA-1 |
| Gene Sequence |
| Protein sequence |
