AlfalfaGEDB | Msa0161280

M. sativa cultivar zhongmu-4 / Msa0161280

KEGG: -
GO: -
PFAM: PF13912.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0161280	A0A072VU80	43.777	233	112	5	18	243	16	236	2.51e-35	142
Msa0161280	A0A072VU80	71.315	251	43	5	250	491	16	246	5.51e-108	331

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TFs/TRs:

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Gene ID	Type	Classification
Msa0161280	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0161280	MtrunA17_Chr1g0174861	71.315	251	43	5	250	491	16	246	1.06e-111	331
Msa0161280	MtrunA17_Chr1g0174861	43.777	233	112	5	18	243	16	236	4.84e-39	142
Msa0161280	MtrunA17_Chr1g0174871	60.153	261	85	7	265	509	28	285	1.62e-85	265
Msa0161280	MtrunA17_Chr1g0174871	62.868	272	80	5	1	256	1	267	1.62e-84	263
Msa0161280	MtrunA17_Chr1g0169521	35.135	296	136	9	259	508	30	315	5.68e-38	141
Msa0161280	MtrunA17_Chr1g0169561	34.680	297	134	8	259	507	30	314	7.86e-38	141
Msa0161280	MtrunA17_Chr1g0174001	39.500	200	87	7	337	508	1	194	3.31e-27	108
Msa0161280	MtrunA17_Chr1g0172961	34.711	242	121	9	259	469	23	258	2.66e-26	107
Msa0161280	MtrunA17_Chr1g0172961	28.947	228	131	10	28	227	30	254	7.40e-12	65.9
Msa0161280	MtrunA17_Chr1g0172861	31.522	276	135	12	259	503	23	275	8.60e-23	98.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 94 sgRNAs with CRISPR-Local

Find 444 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAACAAGTTGGTGGTG+AGG	0.597114	1_4:-36146459	Msa0161280:CDS
AAAAGGAGTGTTGTTGATCA+TGG	0.562216	1_4:-36147039	Msa0161280:CDS
AAACAATGAAAAGAACAAGT+TGG	0.564169	1_4:-36146467	Msa0161280:CDS
AAACAATGAAAAGAACAGGT+TGG	0.620052	1_4:-36140012	Msa0161280:CDS
AAAGACAAACATGCTGATGA+TGG	0.542758	1_4:-36140401	Msa0161280:CDS
AAATACTTTCTCAAATCAAT+TGG	0.400225	1_4:+36146736	None:intergenic
AATGAAGAGAACAACAACAC+TGG	0.663304	1_4:-36146367	Msa0161280:CDS
ACAACTGGTAACGCCCTCGG+CGG	0.717230	1_4:-36146976	Msa0161280:CDS
ACAACTGGTAATGCCCTCGG+CGG	0.704742	1_4:-36140473	Msa0161280:CDS
ACATTGTCATCATTGGAGGC+AGG	0.544139	1_4:+36139930	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTCTCATATCAAACCAAAA+AGG	-	chr1_4:36139840-36139859	Msa0161280:CDS	30.0%
AACAAAGAAAAACAAGCTGA+TGG	-	chr1_4:36139989-36140008	Msa0161280:CDS	30.0%
TACTTTCTCAAATCAATTGG+TGG	+	chr1_4:36140160-36140179	None:intergenic	30.0%
ATCAAACTTTGCAGAAATGT+GGG	-	chr1_4:36140322-36140341	Msa0161280:CDS	30.0%
GTTCTCTTCATTATCATCAT+TGG	+	chr1_4:36140521-36140540	None:intergenic	30.0%
TTGTTGTGATAATTTCATCG+TGG	-	chr1_4:36140862-36140881	Msa0161280:intron	30.0%
AATATGGAGTTGAATGTTGA+TGG	-	chr1_4:36141045-36141064	Msa0161280:intron	30.0%
ACTTCAATGACAAATAATGG+TGG	-	chr1_4:36141477-36141496	Msa0161280:intron	30.0%
TTTGATTGTAACTACTCTTG+TGG	-	chr1_4:36141681-36141700	Msa0161280:intron	30.0%
GTAGAAGAATAATTCTCTAG+TGG	-	chr1_4:36141716-36141735	Msa0161280:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	36139800	36147118	36139800	ID=Msa0161280;Name=Msa0161280
chr1_4	mRNA	36139800	36147118	36139800	ID=Msa0161280-mRNA-1;Parent=Msa0161280;Name=Msa0161280-mRNA-1;_AED=0.07;_eAED=0.18;_QI=0\|0\|0\|1\|0\|0\|5\|0\|510
chr1_4	exon	36146908	36147118	36146908	ID=Msa0161280-mRNA-1:exon:10752;Parent=Msa0161280-mRNA-1
chr1_4	exon	36146791	36146899	36146791	ID=Msa0161280-mRNA-1:exon:10751;Parent=Msa0161280-mRNA-1
chr1_4	exon	36146575	36146786	36146575	ID=Msa0161280-mRNA-1:exon:10750;Parent=Msa0161280-mRNA-1
chr1_4	exon	36146324	36146541	36146324	ID=Msa0161280-mRNA-1:exon:10749;Parent=Msa0161280-mRNA-1
chr1_4	exon	36139800	36140582	36139800	ID=Msa0161280-mRNA-1:exon:10748;Parent=Msa0161280-mRNA-1
chr1_4	CDS	36146908	36147118	36146908	ID=Msa0161280-mRNA-1:cds;Parent=Msa0161280-mRNA-1
chr1_4	CDS	36146791	36146899	36146791	ID=Msa0161280-mRNA-1:cds;Parent=Msa0161280-mRNA-1
chr1_4	CDS	36146575	36146786	36146575	ID=Msa0161280-mRNA-1:cds;Parent=Msa0161280-mRNA-1
chr1_4	CDS	36146324	36146541	36146324	ID=Msa0161280-mRNA-1:cds;Parent=Msa0161280-mRNA-1
chr1_4	CDS	36139800	36140582	36139800	ID=Msa0161280-mRNA-1:cds;Parent=Msa0161280-mRNA-1

Gene Sequence

>Msa0161280

ATGAAGCATTTGTTTTCTTCTGCTTCTTCTTCTTCTTCTCACTCTCATATCAAACCAAAAAGGAGTGTTGTTGATCATGGTGCAAAACTTCACAAATGTGATTTGTGTGACAGAGAATTCACAACTGGTAACGCCCTCGGCGGCCACAAAGCATTCCATAGAGGCAACAACTTTCTTCTAGGAAAAATCAACAAAGAAAAACAAGCTGATGATCGAAAACAACCATTCATGTGTTCGTTGCAGCAAGACATTTTCATCTGTCAATGCTCTCAACGGACACATGAAATCGCACAGCCAATATGGTTCGACTTCGAAGAAGGTAAGATTTATGACCTGGATCAATTTCCACCAATTGATTTGAGAAAGTATTTGCCACCAATGAGTTATGTGACCAAGAAACGGAGCTGGAATTATGTTATTAATGTTGAATCTCATGTTGCAAAAGCAATGCTGGATATGTCACAGCGATCACCTAACAACCTTGATGGGAATCACCGTGTCACGAAAAGGATCAAACTTTGCAGAAATGTGGGTATTGTGATAAAACATTGTGTTGATGAAACAATTGTGAGAGAGAATGATAAAAACAATGAAAAGAACAAGTTGGTGGTGAGGTTAAAGATTCCGAATAGCCTTGTCATTCAAAAATCACAAGCTTCCAATCAATCTGCTTCCAATGATGATAATGAAGAGAACAACAACACTGGTGAAGATGATGAAGCAGGTGAGGAACCAAACAACACTGGTGAACCAAAAACCAATGTTGTTGATCATGAAAATGAGAAACATCAACAAGGTGCAAGACTTCACAAATGTGATTTGTGTGCTAGAGAATTCACAACTGGTAATGCCCTCGGCGGCCACAAACAATTCCATAGCACCAACAACTTTCTTAAAGGTAAAATCAACAAAGACAAACATGCTGATGATGGTATTAAGATCGGAACCAACCATTCATGTGTTCTTTGCAACAAGGCTTTTTCATCGAAAAAAGCTTTGAATGAACACATGAAATGGCACAGCCAAAATGGTTCGAAGAAGGGTAGTAACAATTACGACCTAGATCAGTTTCCATCCATTGATCTGACAAAGTATTTGCCACCAATAAGATACGAGACCAAGAAACGGAGCTGGGATTATATTCTTGATGTTGAAACTGTTAACATTGCTGAAACAATGGTGGAAATGTCTCAGCTATCATCTGCCAATCTTAATGTGAATCACCGTGACTGTAAAAGGATGAAACTTTGCAGCAATGTTGATGATGATGATGTGAAGATCAATGAACAAGGTGGTAAAAACAATGAAAAGAACAGGTTGGTGGTGAGATTAAAGATTCCAAAAGGACTTATTACTCAAACATCTCAAGCTAGCAAGCAACCTGCCTCCAATGATGACAATGTAGAGAACAATACCGATGAAGGTGATGAAACGGATGGGGAATCAACTCTTGAATTCGAGTCAAACATTTTTCAGGATTTTGATTTGAATGAGCTACCGGCAAATGATGTTGTAGATGAAACCACTATTTAG

Protein sequence

>Msa0161280

MKHLFSSASSSSSHSHIKPKRSVVDHGAKLHKCDLCDREFTTGNALGGHKAFHRGNNFLLGKINKEKQADDRKQPFMCSLQQDIFICQCSQRTHEIAQPIWFDFEEGKIYDLDQFPPIDLRKYLPPMSYVTKKRSWNYVINVESHVAKAMLDMSQRSPNNLDGNHRVTKRIKLCRNVGIVIKHCVDETIVRENDKNNEKNKLVVRLKIPNSLVIQKSQASNQSASNDDNEENNNTGEDDEAGEEPNNTGEPKTNVVDHENEKHQQGARLHKCDLCAREFTTGNALGGHKQFHSTNNFLKGKINKDKHADDGIKIGTNHSCVLCNKAFSSKKALNEHMKWHSQNGSKKGSNNYDLDQFPSIDLTKYLPPIRYETKKRSWDYILDVETVNIAETMVEMSQLSSANLNVNHRDCKRMKLCSNVDDDDVKINEQGGKNNEKNRLVVRLKIPKGLITQTSQASKQPASNDDNVENNTDEGDETDGESTLEFESNIFQDFDLNELPANDVVDETTI*