AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0161350

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0161350 MtrunA17_Chr1g0175091 94.872 429 19 3 1 428 1 427 0.0 790
Msa0161350 MtrunA17_Chr7g0258551 60.976 369 113 9 67 421 20 371 1.11e-132 387
Msa0161350 MtrunA17_Chr7g0261911 54.118 170 69 3 249 409 252 421 7.56e-56 192
Msa0161350 MtrunA17_Chr2g0291021 50.296 169 66 3 259 409 49 217 6.81e-50 172
Msa0161350 MtrunA17_Chr1g0190301 54.878 164 60 4 254 403 166 329 7.63e-50 173
Msa0161350 MtrunA17_Chr7g0254981 51.282 156 63 4 250 392 33 188 1.11e-48 167
Msa0161350 MtrunA17_Chr7g0274471 53.333 165 65 4 258 414 217 377 4.56e-48 170
Msa0161350 MtrunA17_Chr1g0175081 72.222 90 25 0 241 330 4 93 1.55e-39 137
Msa0161350 MtrunA17_Chr6g0461641 45.562 169 66 3 255 401 38 202 9.46e-39 144
Msa0161350 MtrunA17_Chr2g0321461 44.086 186 73 5 237 400 27 203 4.25e-38 142
Msa0161350 MtrunA17_Chr1g0175071 43.350 203 107 4 195 395 118 314 2.69e-37 138
Msa0161350 MtrunA17_Chr7g0242521 50.365 137 63 3 247 379 2 137 7.84e-36 132
Msa0161350 MtrunA17_Chr5g0409731 45.455 143 64 2 259 387 25 167 3.00e-34 129
Msa0161350 MtrunA17_Chr5g0417521 39.073 151 78 1 255 391 11 161 5.05e-33 127
Msa0161350 MtrunA17_Chr4g0042451 42.282 149 71 3 255 389 13 160 6.61e-33 127
Msa0161350 MtrunA17_Chr2g0290991 40.373 161 73 4 240 382 21 176 5.63e-31 120
Msa0161350 MtrunA17_Chr8g0356501 42.657 143 71 3 255 389 33 172 2.85e-30 119
Msa0161350 MtrunA17_Chr2g0310011 45.522 134 62 3 255 380 31 161 4.63e-30 119
Msa0161350 MtrunA17_Chr7g0258531 35.897 234 115 7 163 389 139 344 3.47e-29 117
Msa0161350 MtrunA17_Chr1g0197171 40.288 139 64 3 259 382 44 178 1.95e-26 108
Msa0161350 MtrunA17_Chr6g0479431 57.377 61 26 0 261 321 204 264 9.53e-17 81.3
Msa0161350 MtrunA17_Chr7g0243081 52.055 73 34 1 261 333 73 144 2.03e-16 80.1
Msa0161350 MtrunA17_Chr2g0320231 58.209 67 27 1 261 327 99 164 2.28e-16 80.5
Msa0161350 MtrunA17_Chr3g0109981 57.377 61 26 0 261 321 276 336 2.89e-16 80.5
Msa0161350 MtrunA17_Chr8g0379121 56.061 66 28 1 257 322 109 173 5.64e-16 79.7
Msa0161350 MtrunA17_Chr8g0366281 55.738 61 27 0 261 321 175 235 1.24e-15 77.8
Msa0161350 MtrunA17_Chr5g0410741 60.000 55 22 0 261 315 221 275 1.89e-15 77.8
Msa0161350 MtrunA17_Chr1g0162931 62.069 58 21 1 256 312 14 71 3.33e-15 75.9
Msa0161350 MtrunA17_Chr1g0210911 63.462 52 19 0 261 312 24 75 7.53e-15 75.5
Msa0161350 MtrunA17_Chr2g0288601 62.264 53 20 0 257 309 69 121 7.67e-15 70.9
Msa0161350 MtrunA17_Chr3g0095611 54.237 59 27 0 257 315 17 75 1.20e-14 72.8
Msa0161350 MtrunA17_Chr1g0154261 52.239 67 31 1 250 315 116 182 1.33e-14 73.9
Msa0161350 MtrunA17_Chr4g0062921 43.478 92 47 1 230 321 211 297 1.30e-13 72.4
Msa0161350 MtrunA17_Chr2g0283951 58.491 53 22 0 257 309 87 139 3.47e-13 70.9
Msa0161350 MtrunA17_Chr7g0275401 54.098 61 27 1 252 312 185 244 6.09e-13 68.9
Msa0161350 MtrunA17_Chr1g0198601 51.667 60 29 0 259 318 211 270 1.43e-12 68.9
Msa0161350 MtrunA17_Chr4g0009901 50.877 57 27 1 257 313 166 221 3.35e-12 66.6
Msa0161350 MtrunA17_Chr4g0045361 53.448 58 27 0 256 313 271 328 1.01e-11 66.2
Msa0161350 MtrunA17_Chr5g0400241 45.570 79 39 2 240 315 289 366 1.44e-11 66.2
Msa0161350 MtrunA17_Chr5g0422621 41.304 92 48 2 227 313 162 252 1.93e-11 65.1
Msa0161350 MtrunA17_Chr5g0402631 54.386 57 26 0 257 313 212 268 2.28e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0161350 AT5G06800.1 80.000 135 26 1 259 392 192 326 2.89e-70 227
Msa0161350 AT5G06800.2 77.143 140 26 2 259 392 192 331 8.87e-68 220
Msa0161350 AT4G28610.1 56.522 161 63 4 242 399 212 368 4.86e-52 180
Msa0161350 AT2G01060.1 53.086 162 70 4 254 412 10 168 2.24e-50 172
Msa0161350 AT5G29000.1 58.571 140 53 2 254 388 183 322 2.24e-50 174
Msa0161350 AT5G29000.3 58.571 140 53 2 254 388 183 322 2.24e-50 174
Msa0161350 AT5G29000.2 58.571 140 53 2 254 388 226 365 2.77e-50 176
Msa0161350 AT3G04450.4 45.588 204 93 6 211 409 157 347 7.13e-50 174
Msa0161350 AT3G04450.2 45.588 204 93 6 211 409 157 347 7.13e-50 174
Msa0161350 AT3G04450.1 45.588 204 93 6 211 409 197 387 1.41e-49 174
Msa0161350 AT3G13040.3 46.597 191 90 5 232 411 211 400 7.89e-46 164
Msa0161350 AT3G13040.1 46.597 191 90 5 232 411 211 400 7.89e-46 164
Msa0161350 AT3G13040.2 46.597 191 90 5 232 411 211 400 7.89e-46 164
Msa0161350 AT2G20400.1 56.923 130 54 2 259 386 231 360 3.05e-43 156
Msa0161350 AT2G20400.2 56.489 131 54 3 259 386 231 361 1.62e-41 152
Msa0161350 AT5G18240.1 43.976 166 76 2 255 403 41 206 1.06e-37 141
Msa0161350 AT5G18240.4 43.976 166 76 2 255 403 41 206 1.06e-37 141
Msa0161350 AT3G04030.3 48.466 163 62 4 255 400 41 198 4.25e-37 140
Msa0161350 AT1G69580.1 45.806 155 63 3 255 389 30 183 2.00e-36 136
Msa0161350 AT5G06800.3 90.769 65 6 0 259 323 192 256 3.40e-36 134
Msa0161350 AT1G69580.2 44.516 155 66 2 255 389 30 184 6.83e-36 135
Msa0161350 AT3G24120.1 42.515 167 75 2 244 389 26 192 1.81e-35 133
Msa0161350 AT4G13640.1 42.515 167 80 3 238 388 14 180 4.01e-35 132
Msa0161350 AT5G18240.5 43.373 166 75 3 255 403 41 204 1.14e-34 133
Msa0161350 AT1G79430.2 46.099 141 69 3 255 389 30 169 2.82e-34 131
Msa0161350 AT3G04030.2 47.239 163 63 5 255 400 41 197 4.21e-34 131
Msa0161350 AT3G24120.2 41.765 170 75 3 244 389 26 195 1.91e-33 127
Msa0161350 AT4G13640.2 41.765 170 80 4 238 388 14 183 3.77e-33 127
Msa0161350 AT5G45580.1 42.500 160 62 4 259 393 7 161 1.43e-32 124
Msa0161350 AT5G45580.2 42.500 160 62 4 259 393 23 177 1.86e-32 124
Msa0161350 AT5G18240.2 41.566 166 74 3 255 403 41 200 2.59e-32 127
Msa0161350 AT5G18240.3 41.566 166 74 3 255 403 41 200 2.59e-32 127
Msa0161350 AT3G04030.1 46.012 163 60 5 255 400 41 192 8.86e-32 125
Msa0161350 AT2G01060.2 51.639 122 53 4 294 412 1 119 2.50e-30 117
Msa0161350 AT3G04450.3 49.541 109 48 3 211 319 197 298 1.90e-26 108
Msa0161350 AT3G12730.1 40.972 144 69 5 259 389 23 163 4.28e-26 105
Msa0161350 AT5G29000.4 66.667 66 22 0 254 319 226 291 3.26e-25 105
Msa0161350 AT3G04030.5 44.094 127 49 4 291 400 1 122 6.73e-18 84.7
Msa0161350 AT1G79430.1 42.857 105 53 3 291 389 1 104 6.99e-18 84.0
Msa0161350 AT4G04605.1 54.412 68 31 0 248 315 3 70 7.20e-17 79.7
Msa0161350 AT2G42660.1 53.731 67 31 0 257 323 48 114 1.95e-16 79.3
Msa0161350 AT5G16560.1 57.377 61 26 0 261 321 221 281 3.21e-16 80.5
Msa0161350 AT5G42630.2 53.623 69 30 1 261 327 107 175 3.26e-16 77.8
Msa0161350 AT5G42630.1 53.623 69 30 1 261 327 107 175 5.69e-16 78.2
Msa0161350 AT2G40260.1 54.545 77 29 3 252 323 70 145 9.97e-16 79.0
Msa0161350 AT2G02060.2 49.383 81 33 1 240 312 2 82 1.10e-15 77.0
Msa0161350 AT2G02060.1 49.383 81 33 1 240 312 2 82 1.10e-15 77.0
Msa0161350 AT2G06020.1 46.053 76 40 1 240 315 68 142 1.15e-15 77.8
Msa0161350 AT2G38300.1 59.016 61 24 1 256 315 50 110 2.45e-15 77.4
Msa0161350 AT4G17695.1 55.738 61 27 0 261 321 166 226 2.45e-15 77.0
Msa0161350 AT1G32240.2 60.000 55 22 0 261 315 215 269 2.45e-15 77.4
Msa0161350 AT1G14600.1 63.462 52 19 0 261 312 25 76 2.46e-15 75.9
Msa0161350 AT1G32240.1 60.000 55 22 0 261 315 215 269 3.05e-15 77.4
Msa0161350 AT4G04580.1 49.231 65 33 0 248 312 3 67 6.73e-14 69.7
Msa0161350 AT4G37180.1 62.264 53 20 0 261 313 212 264 7.39e-14 73.2
Msa0161350 AT4G37180.2 62.264 53 20 0 261 313 219 271 1.01e-13 72.8
Msa0161350 AT5G18240.6 35.385 130 61 3 291 403 1 124 1.09e-13 72.0
Msa0161350 AT2G03500.1 52.381 63 30 0 259 321 233 295 2.14e-13 72.0
Msa0161350 AT3G04030.4 40.945 127 47 5 291 400 1 116 4.67e-13 70.1
Msa0161350 AT5G58080.1 46.154 78 39 2 252 329 187 261 2.31e-12 69.3
Msa0161350 AT3G16857.3 58.182 55 22 1 259 313 167 220 7.30e-12 67.8
Msa0161350 AT3G16857.2 58.182 55 22 1 259 313 237 290 9.53e-12 67.4
Msa0161350 AT3G16857.1 58.182 55 22 1 259 313 237 290 9.62e-12 67.4
Msa0161350 AT4G04555.1 51.471 68 28 1 253 315 3 70 1.51e-11 61.2
Msa0161350 AT4G37180.3 61.224 49 19 0 261 309 212 260 2.14e-11 64.7

Find 55 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGAGAAATGCTTCAAATTT+TGG 0.060224 1_4:-36561393 Msa0161350:CDS
CCAATTGAACACTCAAAATT+TGG 0.191936 1_4:-36558586 Msa0161350:CDS
TCAATATCTTTAGGACTAAT+TGG 0.223277 1_4:+36558540 None:intergenic
TAAAATCACCATTTAGTATA+TGG 0.227162 1_4:+36561454 None:intergenic
TTCTAATAAGGTTAATGATT+TGG 0.230770 1_4:-36561142 Msa0161350:CDS
TTTCCATTAGAATGAATTAA+TGG 0.240080 1_4:+36560901 None:intergenic
GAGAATAAGATTGATTATTT+AGG 0.265006 1_4:-36561492 Msa0161350:CDS
TCCATTACAAGCAGGGTTTC+CGG 0.269736 1_4:+36560662 None:intergenic
CAACAAAACCATATACTAAA+TGG 0.279757 1_4:-36561462 Msa0161350:CDS
AGTCCATTAATTCATTCTAA+TGG 0.307810 1_4:-36560904 Msa0161350:CDS
AAATTACCTTGAGCTGGTTC+TGG 0.321925 1_4:+36560116 None:intergenic
CAACACCGAAAGCGATATTA+AGG 0.350678 1_4:-36560214 Msa0161350:CDS
ACTCAAAATTGTGGCTAGAT+TGG 0.356646 1_4:+36561070 None:intergenic
GGAAATGTCTCAACTAATTC+CGG 0.366513 1_4:-36560681 Msa0161350:CDS
ACATTTCCTGTTGACATAGT+TGG 0.376397 1_4:+36560696 None:intergenic
ATGGTAACCTCAATATCTTT+AGG 0.378705 1_4:+36558531 None:intergenic
ATCAGCCTTAATATCGCTTT+CGG 0.385906 1_4:+36560209 None:intergenic
GAAAATTGAGATATCAAAAG+AGG 0.399023 1_4:+36561165 None:intergenic
CCACAATATGATTACCAAGT+TGG 0.422118 1_4:-36561204 Msa0161350:CDS
GGAATTCCCTTTGTATCAAA+GGG 0.429673 1_4:-36561121 Msa0161350:CDS
CTGTCTCCAACTATGTCAAC+AGG 0.441628 1_4:-36560702 Msa0161350:CDS
TACTACTTTCATTTCCAACT+TGG 0.455279 1_4:+36561190 None:intergenic
TGGAATTCCCTTTGTATCAA+AGG 0.460488 1_4:-36561122 Msa0161350:CDS
TCCGGAAACCCTGCTTGTAA+TGG 0.462303 1_4:-36560663 Msa0161350:CDS
CTGAAGTTGGTGACTCAAAA+CGG 0.487919 1_4:+36561259 None:intergenic
ATTGGTTTGATGTTGAATCA+AGG 0.496222 1_4:+36561088 None:intergenic
ATAACAAAATTACCTTGAGC+TGG 0.498627 1_4:+36560110 None:intergenic
AAAATTACAGTTGCGAATAG+AGG 0.499177 1_4:-36558667 Msa0161350:CDS
CCAACTTGGTAATCATATTG+TGG 0.502891 1_4:+36561204 None:intergenic
AATTAGTCCTAAAGATATTG+AGG 0.504863 1_4:-36558538 Msa0161350:CDS
CTCCAATAAAATTCCCACAT+TGG 0.506700 1_4:+36560989 None:intergenic
CCCTGCTTGTAATGGATCAT+CGG 0.507035 1_4:-36560655 Msa0161350:CDS
GTTGAGTGTGTGAATCGCCT+TGG 0.509079 1_4:-36560582 Msa0161350:CDS
ACCGATGATCCATTACAAGC+AGG 0.527035 1_4:+36560654 None:intergenic
CAAGTAAAACACGAATAAGA+TGG 0.534054 1_4:-36560629 Msa0161350:CDS
CAGTGGCTTGCAAATTAGAG+AGG 0.550038 1_4:-36559491 Msa0161350:CDS
CCGATGATCCATTACAAGCA+GGG 0.564544 1_4:+36560655 None:intergenic
CAAAACCAAAACCAAGGACA+AGG 0.566148 1_4:-36561318 Msa0161350:CDS
TTATGCAACAGAAATTTGCA+TGG 0.566514 1_4:-36561232 Msa0161350:CDS
AACTTGACAACTCAAAATTG+TGG 0.571457 1_4:+36561061 None:intergenic
TATGCAACAGAAATTTGCAT+GGG 0.578681 1_4:-36561231 Msa0161350:CDS
AAAATTGAGATATCAAAAGA+GGG 0.579511 1_4:+36561166 None:intergenic
AGCTGCAGCTAGACGTTCAG+AGG 0.587380 1_4:-36559462 Msa0161350:CDS
GAAAGCGATATTAAGGCTGA+TGG 0.589838 1_4:-36560207 Msa0161350:CDS
GAGTGTGTGAATCGCCTTGG+TGG 0.595652 1_4:-36560579 Msa0161350:CDS
TTAAGGCTGATGGATTCAGA+TGG 0.601499 1_4:-36560197 Msa0161350:CDS
AATCCAATAAAATCCAATGT+GGG 0.615499 1_4:-36561002 Msa0161350:CDS
CAACAAAAGACAAGTAGTAG+TGG 0.616301 1_4:-36558609 Msa0161350:CDS
AAATCCAATAAAATCCAATG+TGG 0.616777 1_4:-36561003 Msa0161350:CDS
GCGTCTTCATGAACAACTAG+AGG 0.636358 1_4:-36559440 Msa0161350:intron
GATGATTGTTCCATAACCAA+TGG 0.643571 1_4:+36561357 None:intergenic
TATATGCCAGAACCAGCTCA+AGG 0.651421 1_4:-36560122 Msa0161350:intron
CAAAGTCAAAACCAAAACCA+AGG 0.666219 1_4:-36561324 Msa0161350:CDS
ACTTACTCTCGGCACCACCA+AGG 0.691778 1_4:+36560565 None:intergenic
CAGTTGCGAATAGAGGAACA+AGG 0.704497 1_4:-36558660 Msa0161350:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAATAAAAGTAAATTATA+AGG + chr1_4:36559158-36559177 None:intergenic 10.0%
!!! TTGTTTCTATTTTCATTTAT+TGG - chr1_4:36559173-36559192 Msa0161350:intron 15.0%
!!! TTCTTGAATGTTTATTTTAT+CGG + chr1_4:36559502-36559521 None:intergenic 15.0%
!!! TTCTCTTATTTTTATAAAAG+TGG + chr1_4:36560123-36560142 None:intergenic 15.0%
!! TTAGAATTTATATTCAGTAA+GGG + chr1_4:36560305-36560324 None:intergenic 15.0%
!!! TTTAGAATTTATATTCAGTA+AGG + chr1_4:36560306-36560325 None:intergenic 15.0%
!! AATTATAGAACATAAACAAT+CGG + chr1_4:36560424-36560443 None:intergenic 15.0%
!! TATTATAATAAACAATCACT+CGG - chr1_4:36560970-36560989 Msa0161350:CDS 15.0%
!! GAGAATAAGATTGATTATTT+AGG - chr1_4:36558506-36558525 Msa0161350:CDS 20.0%
!! TAAAATCACCATTTAGTATA+TGG + chr1_4:36558547-36558566 None:intergenic 20.0%
!! AAAATTGAGATATCAAAAGA+GGG + chr1_4:36558835-36558854 None:intergenic 20.0%
!!! ATCTCAATTTTCTTCTAATA+AGG - chr1_4:36558844-36558863 Msa0161350:intron 20.0%
!! TTCTAATAAGGTTAATGATT+TGG - chr1_4:36558856-36558875 Msa0161350:intron 20.0%
!! TTTCCATTAGAATGAATTAA+TGG + chr1_4:36559100-36559119 None:intergenic 20.0%
!! AAATGTTGTTAGAAAGTAAA+CGG - chr1_4:36559718-36559737 Msa0161350:intron 20.0%
!! TATCTATAATGTAAAGTACA+TGG - chr1_4:36559929-36559948 Msa0161350:intron 20.0%
!! ATTATGAAAAGAAACATAGT+TGG + chr1_4:36560484-36560503 None:intergenic 20.0%
!! ATGTGTATAAATGTAATTGA+AGG + chr1_4:36560589-36560608 None:intergenic 20.0%
!! AAAGTGAGAAAATTAAAGAT+CGG - chr1_4:36560704-36560723 Msa0161350:CDS 20.0%
!! TGAACTTTAAATTTCATAGA+AGG + chr1_4:36560809-36560828 None:intergenic 20.0%
!! GAAAAAAAAATTAAGCGTAA+AGG - chr1_4:36561038-36561057 Msa0161350:CDS 20.0%
! CAACAAAACCATATACTAAA+TGG - chr1_4:36558536-36558555 Msa0161350:CDS 25.0%
!! ATGAGAAATGCTTCAAATTT+TGG - chr1_4:36558605-36558624 Msa0161350:CDS 25.0%
! GAAAATTGAGATATCAAAAG+AGG + chr1_4:36558836-36558855 None:intergenic 25.0%
!! TGGATTTTATTGGATTTTTC+AGG + chr1_4:36558992-36559011 None:intergenic 25.0%
! AAATCCAATAAAATCCAATG+TGG - chr1_4:36558995-36559014 Msa0161350:intron 25.0%
! AATCCAATAAAATCCAATGT+GGG - chr1_4:36558996-36559015 Msa0161350:intron 25.0%
! AGTCCATTAATTCATTCTAA+TGG - chr1_4:36559094-36559113 Msa0161350:intron 25.0%
! CGAAAATTTGATTGTGATAT+TGG - chr1_4:36559744-36559763 Msa0161350:intron 25.0%
! AAGTGAAGATTAAGAATAAC+TGG + chr1_4:36560245-36560264 None:intergenic 25.0%
!!! TTTCTTTTCATAATTTGCAG+TGG - chr1_4:36560490-36560509 Msa0161350:intron 25.0%
!! CTATGTTTGGATTTTATTAG+AGG - chr1_4:36560783-36560802 Msa0161350:intron 25.0%
! AGTCTAAATTCAACTATAGA+TGG + chr1_4:36560922-36560941 None:intergenic 25.0%
!! TCTAAGACAAGAAAAAACTT+TGG - chr1_4:36561074-36561093 Msa0161350:CDS 25.0%
!! TTTTTATGTCTTAGAGACAA+CGG + chr1_4:36561191-36561210 None:intergenic 25.0%
!!! TCAATATCTTTAGGACTAAT+TGG + chr1_4:36561461-36561480 None:intergenic 25.0%
! AATTAGTCCTAAAGATATTG+AGG - chr1_4:36561460-36561479 Msa0161350:CDS 25.0%
!!! GGTGATTTTAATTCTGAGTT+TGG - chr1_4:36558557-36558576 Msa0161350:CDS 30.0%
!! TGGAAATTTTAGTCAACCAT+TGG - chr1_4:36558625-36558644 Msa0161350:CDS 30.0%
!! TTTTAGTCAACCATTGGTTA+TGG - chr1_4:36558631-36558650 Msa0161350:CDS 30.0%
TTATGCAACAGAAATTTGCA+TGG - chr1_4:36558766-36558785 Msa0161350:intron 30.0%
TATGCAACAGAAATTTGCAT+GGG - chr1_4:36558767-36558786 Msa0161350:intron 30.0%
TACTACTTTCATTTCCAACT+TGG + chr1_4:36558811-36558830 None:intergenic 30.0%
! GATTTTCCCTTTGATACAAA+GGG + chr1_4:36558886-36558905 None:intergenic 30.0%
! TGATTTTCCCTTTGATACAA+AGG + chr1_4:36558887-36558906 None:intergenic 30.0%
!! ATTGGTTTGATGTTGAATCA+AGG + chr1_4:36558913-36558932 None:intergenic 30.0%
AACTTGACAACTCAAAATTG+TGG + chr1_4:36558940-36558959 None:intergenic 30.0%
!! TTTTCAGGAGATCTAAGAAT+TGG + chr1_4:36558977-36558996 None:intergenic 30.0%
! ATTCCCACATTGGATTTTAT+TGG + chr1_4:36559002-36559021 None:intergenic 30.0%
! ATCCAATGTGGGAATTTTAT+TGG - chr1_4:36559007-36559026 Msa0161350:intron 30.0%
! TGTGGTTCATATGATTTTCA+TGG - chr1_4:36559239-36559258 Msa0161350:intron 30.0%
CAAGTAAAACACGAATAAGA+TGG - chr1_4:36559369-36559388 Msa0161350:intron 30.0%
!! TAAATTCATCGGTTTACTTC+TGG - chr1_4:36559455-36559474 Msa0161350:CDS 30.0%
AGAGAGAAGATGAAAGATAA+CGG + chr1_4:36559651-36559670 None:intergenic 30.0%
! TCTGCAAATGACTTTTAACA+TGG + chr1_4:36559839-36559858 None:intergenic 30.0%
ATAACAAAATTACCTTGAGC+TGG + chr1_4:36559891-36559910 None:intergenic 30.0%
AAACTAATCTACAGTAGAGT+TGG - chr1_4:36560035-36560054 Msa0161350:intron 30.0%
AACTAAGTGCTTATAGCTTA+AGG - chr1_4:36560069-36560088 Msa0161350:intron 30.0%
! AAAGTCAGACTGTTTTCAAT+AGG - chr1_4:36560146-36560165 Msa0161350:CDS 30.0%
ATTCTTAATCTTCACTTCGA+TGG - chr1_4:36560248-36560267 Msa0161350:intron 30.0%
TCGATGTGTTATAATCAATC+AGG - chr1_4:36560753-36560772 Msa0161350:CDS 30.0%
CGATGTGTTATAATCAATCA+GGG - chr1_4:36560754-36560773 Msa0161350:CDS 30.0%
AAGAAAAAACTTTGGATTCG+AGG - chr1_4:36561082-36561101 Msa0161350:CDS 30.0%
ATATCGGATATGTTTCTCTA+AGG + chr1_4:36561117-36561136 None:intergenic 30.0%
GAGAAACATATCCGATATTA+CGG - chr1_4:36561119-36561138 Msa0161350:CDS 30.0%
ACAGAAATTCTCCGTAATAT+CGG + chr1_4:36561133-36561152 None:intergenic 30.0%
! ACTGTAATTTTCTCTGAATC+TGG + chr1_4:36561322-36561341 None:intergenic 30.0%
AAAATTACAGTTGCGAATAG+AGG - chr1_4:36561331-36561350 Msa0161350:CDS 30.0%
!! TACTACTTGTCTTTTGTTGT+TGG + chr1_4:36561388-36561407 None:intergenic 30.0%
!! CCAAATTTTGAGTGTTCAAT+TGG + chr1_4:36561415-36561434 None:intergenic 30.0%
CCAATTGAACACTCAAAATT+TGG - chr1_4:36561412-36561431 Msa0161350:CDS 30.0%
ATGGTAACCTCAATATCTTT+AGG + chr1_4:36561470-36561489 None:intergenic 30.0%
!! ATTGAGGTTACCATTTTTGA+AGG - chr1_4:36561476-36561495 Msa0161350:CDS 30.0%
!! TTGAGGTTACCATTTTTGAA+GGG - chr1_4:36561477-36561496 Msa0161350:CDS 30.0%
GATGATTGTTCCATAACCAA+TGG + chr1_4:36558644-36558663 None:intergenic 35.0%
!!! CTTGGTTTTGGTTTTGACTT+TGG + chr1_4:36558676-36558695 None:intergenic 35.0%
CAAAGTCAAAACCAAAACCA+AGG - chr1_4:36558674-36558693 Msa0161350:CDS 35.0%
! ATTTTCCTTGTCCTTGGTTT+TGG + chr1_4:36558688-36558707 None:intergenic 35.0%
! ATGATGATTTTCCTTGTCCT+TGG + chr1_4:36558694-36558713 None:intergenic 35.0%
GTTGCATAAAAAGCTGAAGT+TGG + chr1_4:36558755-36558774 None:intergenic 35.0%
! CCAACTTGGTAATCATATTG+TGG + chr1_4:36558797-36558816 None:intergenic 35.0%
CCACAATATGATTACCAAGT+TGG - chr1_4:36558794-36558813 Msa0161350:intron 35.0%
TGGAATTCCCTTTGTATCAA+AGG - chr1_4:36558876-36558895 Msa0161350:intron 35.0%
GGAATTCCCTTTGTATCAAA+GGG - chr1_4:36558877-36558896 Msa0161350:intron 35.0%
ACTCAAAATTGTGGCTAGAT+TGG + chr1_4:36558931-36558950 None:intergenic 35.0%
CTCCAATAAAATTCCCACAT+TGG + chr1_4:36559012-36559031 None:intergenic 35.0%
! TCGAATCTTTCGTGTTTTGT+AGG - chr1_4:36559211-36559230 Msa0161350:intron 35.0%
ACATTTCCTGTTGACATAGT+TGG + chr1_4:36559305-36559324 None:intergenic 35.0%
GGAAATGTCTCAACTAATTC+CGG - chr1_4:36559317-36559336 Msa0161350:intron 35.0%
CCGATGAATTTACTTACTCT+CGG + chr1_4:36559447-36559466 None:intergenic 35.0%
CCGAGAGTAAGTAAATTCAT+CGG - chr1_4:36559444-36559463 Msa0161350:CDS 35.0%
ATCAGCCTTAATATCGCTTT+CGG + chr1_4:36559792-36559811 None:intergenic 35.0%
! GTTCTAAGCACTATGTCATA+AGG + chr1_4:36559998-36560017 None:intergenic 35.0%
ACAAACTGCTAACATGTTTC+AGG - chr1_4:36560325-36560344 Msa0161350:intron 35.0%
CAAACTGCTAACATGTTTCA+GGG - chr1_4:36560326-36560345 Msa0161350:intron 35.0%
! CTCTACATGAGTTCTTTTCT+CGG + chr1_4:36560356-36560375 None:intergenic 35.0%
TAAGCGAGAAATGATTGCAT+TGG + chr1_4:36560447-36560466 None:intergenic 35.0%
TAGCGTAATATCAGGTTTCA+TGG + chr1_4:36560661-36560680 None:intergenic 35.0%
GAGTCAAATAGCGTAATATC+AGG + chr1_4:36560669-36560688 None:intergenic 35.0%
ATCAGGGACAAATCTATGTT+TGG - chr1_4:36560770-36560789 Msa0161350:intron 35.0%
TCGGAAAAACATATTTCCTC+TGG - chr1_4:36560989-36561008 Msa0161350:CDS 35.0%
AATTGTCTATGAATGCGATC+TGG + chr1_4:36561231-36561250 None:intergenic 35.0%
CAACAAAAGACAAGTAGTAG+TGG - chr1_4:36561389-36561408 Msa0161350:CDS 35.0%
AGAATGAGACCCTTCAAAAA+TGG + chr1_4:36561489-36561508 None:intergenic 35.0%
CAAAACCAAAACCAAGGACA+AGG - chr1_4:36558680-36558699 Msa0161350:intron 40.0%
!! CTGAAGTTGGTGACTCAAAA+CGG + chr1_4:36558742-36558761 None:intergenic 40.0%
! CGTGTTTTGTAGGTTTCTTG+TGG - chr1_4:36559221-36559240 Msa0161350:intron 40.0%
! TATTGGCTGCTTGATTGTAG+AGG - chr1_4:36559761-36559780 Msa0161350:intron 40.0%
CAACACCGAAAGCGATATTA+AGG - chr1_4:36559784-36559803 Msa0161350:intron 40.0%
GAAAGCGATATTAAGGCTGA+TGG - chr1_4:36559791-36559810 Msa0161350:intron 40.0%
!! TTAAGGCTGATGGATTCAGA+TGG - chr1_4:36559801-36559820 Msa0161350:intron 40.0%
! AAATTACCTTGAGCTGGTTC+TGG + chr1_4:36559885-36559904 None:intergenic 40.0%
TGACATAGTGCTTAGAACTC+AGG - chr1_4:36560000-36560019 Msa0161350:intron 40.0%
GACATAGTGCTTAGAACTCA+GGG - chr1_4:36560001-36560020 Msa0161350:intron 40.0%
ACATAGTGCTTAGAACTCAG+GGG - chr1_4:36560002-36560021 Msa0161350:intron 40.0%
TGTGACTCTGCTTAAGAACA+AGG - chr1_4:36560614-36560633 Msa0161350:CDS 40.0%
TCTGTCAAATGTGACTGATG+TGG - chr1_4:36561148-36561167 Msa0161350:CDS 40.0%
CTGTCTCCAACTATGTCAAC+AGG - chr1_4:36559296-36559315 Msa0161350:intron 45.0%
TCCATTACAAGCAGGGTTTC+CGG + chr1_4:36559339-36559358 None:intergenic 45.0%
CCGATGATCCATTACAAGCA+GGG + chr1_4:36559346-36559365 None:intergenic 45.0%
!! CCCTGCTTGTAATGGATCAT+CGG - chr1_4:36559343-36559362 Msa0161350:intron 45.0%
ACCGATGATCCATTACAAGC+AGG + chr1_4:36559347-36559366 None:intergenic 45.0%
TATATGCCAGAACCAGCTCA+AGG - chr1_4:36559876-36559895 Msa0161350:intron 45.0%
! CAGTGGCTTGCAAATTAGAG+AGG - chr1_4:36560507-36560526 Msa0161350:intron 45.0%
GCGTCTTCATGAACAACTAG+AGG - chr1_4:36560558-36560577 Msa0161350:intron 45.0%
AGACTACAATTCGCAACCAG+AGG + chr1_4:36561008-36561027 None:intergenic 45.0%
AGCGTAAAGGAACGACATTC+AGG - chr1_4:36561051-36561070 Msa0161350:CDS 45.0%
ATGTCTTAGAGACAACGGTG+TGG + chr1_4:36561186-36561205 None:intergenic 45.0%
CAGTTGCGAATAGAGGAACA+AGG - chr1_4:36561338-36561357 Msa0161350:CDS 45.0%
TCCGGAAACCCTGCTTGTAA+TGG - chr1_4:36559335-36559354 Msa0161350:intron 50.0%
GTTGAGTGTGTGAATCGCCT+TGG - chr1_4:36559416-36559435 Msa0161350:intron 50.0%
CAACGGTGTGGCAATAATCG+AGG + chr1_4:36561174-36561193 None:intergenic 50.0%
CGATCTGGACTGCAATATCG+AGG + chr1_4:36561216-36561235 None:intergenic 50.0%
GAGTGTGTGAATCGCCTTGG+TGG - chr1_4:36559419-36559438 Msa0161350:intron 55.0%
ACTTACTCTCGGCACCACCA+AGG + chr1_4:36559436-36559455 None:intergenic 55.0%
AGCTGCAGCTAGACGTTCAG+AGG - chr1_4:36560536-36560555 Msa0161350:intron 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 36558500 36561520 36558500 ID=Msa0161350;Name=Msa0161350
chr1_4 mRNA 36558500 36561520 36558500 ID=Msa0161350-mRNA-1;Parent=Msa0161350;Name=Msa0161350-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|1|0.66|1|1|1|6|0|428
chr1_4 exon 36560888 36561520 36560888 ID=Msa0161350-mRNA-1:exon:10774;Parent=Msa0161350-mRNA-1
chr1_4 exon 36560571 36560787 36560571 ID=Msa0161350-mRNA-1:exon:10773;Parent=Msa0161350-mRNA-1
chr1_4 exon 36560123 36560239 36560123 ID=Msa0161350-mRNA-1:exon:10772;Parent=Msa0161350-mRNA-1
chr1_4 exon 36559622 36559673 36559622 ID=Msa0161350-mRNA-1:exon:10771;Parent=Msa0161350-mRNA-1
chr1_4 exon 36559441 36559510 36559441 ID=Msa0161350-mRNA-1:exon:10770;Parent=Msa0161350-mRNA-1
chr1_4 exon 36558500 36558697 36558500 ID=Msa0161350-mRNA-1:exon:10769;Parent=Msa0161350-mRNA-1
chr1_4 CDS 36560888 36561520 36560888 ID=Msa0161350-mRNA-1:cds;Parent=Msa0161350-mRNA-1
chr1_4 CDS 36560571 36560787 36560571 ID=Msa0161350-mRNA-1:cds;Parent=Msa0161350-mRNA-1
chr1_4 CDS 36560123 36560239 36560123 ID=Msa0161350-mRNA-1:cds;Parent=Msa0161350-mRNA-1
chr1_4 CDS 36559622 36559673 36559622 ID=Msa0161350-mRNA-1:cds;Parent=Msa0161350-mRNA-1
chr1_4 CDS 36559441 36559510 36559441 ID=Msa0161350-mRNA-1:cds;Parent=Msa0161350-mRNA-1
chr1_4 CDS 36558500 36558697 36558500 ID=Msa0161350-mRNA-1:cds;Parent=Msa0161350-mRNA-1
Gene Sequence

>Msa0161350

ATGAATGAGAATAAGATTGATTATTTAGGAAGTATTCAACAAAACCATATACTAAATGGTGATTTTAATTCTGAGTTTGGAAATTGTTCTAGTCAATATTTTGATATGAGAAATGCTTCAAATTTTGGAAATTTTAGTCAACCATTGGTTATGGAACAATCATCATACATATTCCAAAGTCAAAACCAAAACCAAGGACAAGGAAAATCATCATCATCATCATCAAGCACAATTATGAGCCGTTTTGAGTCACCAACTTCAGCTTTTTATGCAACAGAAATTTGCATGGGTTTTCCACAATATGATTACCAAGTTGGAAATGAAAGTAGTAACCCTCTTTTGATATCTCAATTTTCTTCTAATAAGGTTAATGATTTGGAATTCCCTTTGTATCAAAGGGAAAATCATTACCTTGATTCAACATCAAACCAATCTAGCCACAATTTTGAGTTGTCAAGTTTAAACACTTTGCAACCAATTCTTAGATCTCCTGAAAAATCCAATAAAATCCAATGTGGGAATTTTATTGGAGAAAATTTTCTCTCAGTTGAGCAAAATAAATTCTTCATTGATGATGCTGCATCAGTTAGTATGAGTCCATTAATTCATTCTAATGGAAATCAAGATCATAAAGTTTCTTGTGGTTCATATGATTTTCATGGTTCACAGCTGAATTTCTCTTATCAACAAGATAAACTGTCTCCAACTATGTCAACAGGAAATGTCTCAACTAATTCCGGAAACCCTGCTTGTAATGGATCATCGGTTTCAAGTAAAACACGAATAAGATGGACTCAAGATCTTCATGAGAAGTTTGTTGAGTGTGTGAATCGCCTTGGTGGTGCCGAGAAGGCAACACCGAAAGCGATATTAAGGCTGATGGATTCAGATGGATTGACAATTTTCCATGTTAAAAGTCATTTGCAGAAGTATAGAATTGCAAAATATATGCCAGAACCAGCTCAAGGGAAATCCGAGAAAAGAACTCATGTAGAGAATGTGAATCTTGATGCGAAATCTGGCTTGCAAATTAGAGAGGCACTTCAGCTGCAGCTAGACGTTCAGAGGCGTCTTCATGAACAACTAGAGATTCAGAGAAAATTACAGTTGCGAATAGAGGAACAAGGAAAGCAGTTGAAGATGATGTTTGACCAACAACAAAAGACAAGTAGTAGTGGCCAATTGAACACTCAAAATTTGGACAATACAACAAACAATGATATACCAATTAGTCCTAAAGATATTGAGGTTACCATTTTTGAAGGGTCTCATTCTCAATGCTCATAA
Protein sequence

>Msa0161350

MNENKIDYLGSIQQNHILNGDFNSEFGNCSSQYFDMRNASNFGNFSQPLVMEQSSYIFQSQNQNQGQGKSSSSSSSTIMSRFESPTSAFYATEICMGFPQYDYQVGNESSNPLLISQFSSNKVNDLEFPLYQRENHYLDSTSNQSSHNFELSSLNTLQPILRSPEKSNKIQCGNFIGENFLSVEQNKFFIDDAASVSMSPLIHSNGNQDHKVSCGSYDFHGSQLNFSYQQDKLSPTMSTGNVSTNSGNPACNGSSVSSKTRIRWTQDLHEKFVECVNRLGGAEKATPKAILRLMDSDGLTIFHVKSHLQKYRIAKYMPEPAQGKSEKRTHVENVNLDAKSGLQIREALQLQLDVQRRLHEQLEIQRKLQLRIEEQGKQLKMMFDQQQKTSSSGQLNTQNLDNTTNNDIPISPKDIEVTIFEGSHSQCS*