AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0163800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0163800 MtrunA17_Chr1g0179061 96.266 241 7 2 1 240 1 240 1.50e-170 473
Msa0163800 MtrunA17_Chr2g0311101 81.424 323 23 11 1 311 13 310 6.10e-160 448
Msa0163800 MtrunA17_Chr8g0384141 58.491 53 22 0 181 233 125 177 1.90e-13 69.3
Msa0163800 MtrunA17_Chr3g0134001 58.491 53 22 0 181 233 182 234 1.24e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0163800 AT1G30670.1 44.118 170 93 1 135 304 95 262 9.20e-34 125
Msa0163800 AT2G34820.1 41.315 213 116 3 97 304 81 289 3.32e-29 113
Msa0163800 AT3G21330.1 60.377 53 21 0 181 233 280 332 2.10e-13 70.5
Msa0163800 AT5G01305.1 50.000 58 29 0 181 238 48 105 1.19e-12 65.5
Msa0163800 AT4G00120.1 52.830 53 25 0 181 233 123 175 6.59e-12 63.9

Find 49 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTTGGAATGCTTCAACTTA+TGG 0.195386 1_4:-42142213 Msa0163800:CDS
GACACAAGAGCTTGGGAAAT+TGG 0.201159 1_4:-42142312 Msa0163800:CDS
ATCTCTGCTGTGTTCATCTT+TGG 0.238213 1_4:+42142278 None:intergenic
TTGGTGAAATCTTCCTCATT+TGG 0.290806 1_4:+42142596 None:intergenic
ATCAAAACCTACCATTCTTA+AGG 0.300143 1_4:-42140907 Msa0163800:CDS
TCAAATAGTTAGAGGGAAAA+TGG 0.318116 1_4:+42142771 None:intergenic
AAATAAAACTATTCTTGCTT+TGG 0.325467 1_4:+42140846 None:intergenic
TGGTGAAATCTTCCTCATTT+GGG 0.332786 1_4:+42142597 None:intergenic
TCTCCTCGTAGCATAGCTTT+TGG 0.368098 1_4:+42142552 None:intergenic
AGTTGATCTTGACAAGGGTT+AGG 0.369040 1_4:+42142680 None:intergenic
TCAAAGGAAGCAAAAGGGTT+AGG 0.369513 1_4:+42142461 None:intergenic
AGTAGTGATTTCTAAGTTGT+TGG 0.372241 1_4:+42142835 None:intergenic
GTAGTTTGAATAGGTTGATA+AGG 0.378786 1_4:+42142857 None:intergenic
TACAGTTTCTCTTGAACAAA+TGG 0.383908 1_4:+42140996 None:intergenic
AAGGGTCTGAAACAGCTTGC+TGG 0.388228 1_4:-42140888 Msa0163800:CDS
AATGTGGTGACAAACTCTTT+TGG 0.390869 1_4:+42140951 None:intergenic
AAGTTGTTGGTAGTTTGAAT+AGG 0.402079 1_4:+42142848 None:intergenic
TTGATTATTTCAGGAAGAAA+AGG 0.406675 1_4:-42141060 Msa0163800:intron
GAGCTTGGGAAATTGGTTCC+TGG 0.412191 1_4:-42142305 Msa0163800:CDS
TAGTTTGAATAGGTTGATAA+GGG 0.429054 1_4:+42142858 None:intergenic
AGTTTCATTCAAATAGTTAG+AGG 0.435549 1_4:+42142763 None:intergenic
GTTGATCTTGACAAGGGTTA+GGG 0.453473 1_4:+42142681 None:intergenic
TCAAAACCTACCATTCTTAA+GGG 0.456430 1_4:-42140906 Msa0163800:CDS
CAAAGGAAGCAAAAGGGTTA+GGG 0.461232 1_4:+42142462 None:intergenic
AGAGTATCGCGGCGAGAGAA+AGG 0.466876 1_4:-42142355 Msa0163800:CDS
TCTGCCTCAAAGGAAGCAAA+AGG 0.471457 1_4:+42142455 None:intergenic
CTGAGAAGACACAAGAGCTT+GGG 0.493562 1_4:-42142319 Msa0163800:CDS
TGTAGATCTTCACTTGGAGG+AGG 0.497014 1_4:+42141035 None:intergenic
GTGTTCATCTTTGGTCCACC+AGG 0.499817 1_4:+42142287 None:intergenic
ACTGAGAAGACACAAGAGCT+TGG 0.499993 1_4:-42142320 Msa0163800:CDS
CTGTTTCAGACCCTTAAGAA+TGG 0.516519 1_4:+42140896 None:intergenic
AGCAAAGGGTTCTGCCTCAA+AGG 0.518713 1_4:+42142445 None:intergenic
AGGGTCTGAAACAGCTTGCT+GGG 0.522554 1_4:-42140887 Msa0163800:CDS
GTTTCATTCAAATAGTTAGA+GGG 0.549802 1_4:+42142764 None:intergenic
AAGTTTCTTCAAGCTCAAGT+TGG 0.567049 1_4:-42142230 Msa0163800:CDS
ACTTATGGAAACATTTAGCA+AGG 0.569731 1_4:-42142198 Msa0163800:intron
TTCAGACCCTTAAGAATGGT+AGG 0.583734 1_4:+42140900 None:intergenic
GAGTTCAAAAGCTCTTGTTG+TGG 0.586358 1_4:+42142518 None:intergenic
TCGCGGCGAGAGAAAGGAGA+AGG 0.587504 1_4:-42142349 Msa0163800:CDS
GTGAGAAGAAAGTTGCTGAA+AGG 0.592639 1_4:-42142391 Msa0163800:CDS
CTGCCTCAAAGGAAGCAAAA+GGG 0.606158 1_4:+42142456 None:intergenic
CTTGGGAAATTGGTTCCTGG+TGG 0.620802 1_4:-42142302 Msa0163800:CDS
AATAGTTAGAGGGAAAATGG+TGG 0.625963 1_4:+42142774 None:intergenic
ACAAGTAGTTGATCTTGACA+AGG 0.633389 1_4:+42142674 None:intergenic
CAAGTAGTTGATCTTGACAA+GGG 0.656926 1_4:+42142675 None:intergenic
ATTATTATGATTGCTCAATG+TGG 0.674695 1_4:+42140935 None:intergenic
ATGCTACGAGGAGAAAAGAG+AGG 0.681365 1_4:-42142543 Msa0163800:CDS
TATCTCGTCGCAGAGTATCG+CGG 0.686396 1_4:-42142366 Msa0163800:CDS
ACGCCAAAAGCTATGCTACG+AGG 0.715032 1_4:-42142555 Msa0163800:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTAAATTAAAAAAAAATAT+TGG + chr1_4:42142283-42142302 None:intergenic 0.0%
!! TTTAAATTAAAAAAAAATAT+TGG + chr1_4:42142283-42142302 None:intergenic 0.0%
!!! TAATTAATTTTGCTTAAATA+TGG - chr1_4:42142590-42142609 Msa0163800:CDS 10.0%
!!! TAATTAATTTTGCTTAAATA+TGG - chr1_4:42142590-42142609 Msa0163800:CDS 10.0%
!!! ATTATTTATTTTGTGTTCTT+AGG - chr1_4:42141639-42141658 Msa0163800:intron 15.0%
!! TATTGTTGTAAATATCTAAA+TGG - chr1_4:42141901-42141920 Msa0163800:intron 15.0%
!! AATATATATGTGGATTTATT+TGG + chr1_4:42142619-42142638 None:intergenic 15.0%
!! ATTATTGACAAATATATATG+TGG + chr1_4:42142629-42142648 None:intergenic 15.0%
!!! ATTATTTATTTTGTGTTCTT+AGG - chr1_4:42141639-42141658 Msa0163800:intron 15.0%
!! TATTGTTGTAAATATCTAAA+TGG - chr1_4:42141901-42141920 Msa0163800:intron 15.0%
!! AATATATATGTGGATTTATT+TGG + chr1_4:42142619-42142638 None:intergenic 15.0%
!! ATTATTGACAAATATATATG+TGG + chr1_4:42142629-42142648 None:intergenic 15.0%
!!! TTTTTGAGTTTATAATACCT+AGG - chr1_4:42141749-42141768 Msa0163800:intron 20.0%
!!! TTTTGAGTTTATAATACCTA+GGG - chr1_4:42141750-42141769 Msa0163800:intron 20.0%
!!! TTTTTTATAACAATTCCTCA+TGG + chr1_4:42142047-42142066 None:intergenic 20.0%
!! GGAATTGTTATAAAAAATGA+CGG - chr1_4:42142050-42142069 Msa0163800:intron 20.0%
!!! TCTCTAATTACTTTTTTTTG+TGG - chr1_4:42142447-42142466 Msa0163800:CDS 20.0%
!!! CTCTAATTACTTTTTTTTTG+TGG - chr1_4:42142546-42142565 Msa0163800:CDS 20.0%
!!! TTTTGCTAATTGATTATTTC+AGG - chr1_4:42142681-42142700 Msa0163800:CDS 20.0%
!!! TTTTTGAGTTTATAATACCT+AGG - chr1_4:42141749-42141768 Msa0163800:intron 20.0%
!!! TTTTGAGTTTATAATACCTA+GGG - chr1_4:42141750-42141769 Msa0163800:intron 20.0%
!!! TTTTTTATAACAATTCCTCA+TGG + chr1_4:42142047-42142066 None:intergenic 20.0%
!! GGAATTGTTATAAAAAATGA+CGG - chr1_4:42142050-42142069 Msa0163800:intron 20.0%
!!! TCTCTAATTACTTTTTTTTG+TGG - chr1_4:42142447-42142466 Msa0163800:CDS 20.0%
!!! CTCTAATTACTTTTTTTTTG+TGG - chr1_4:42142546-42142565 Msa0163800:CDS 20.0%
!!! TTTTGCTAATTGATTATTTC+AGG - chr1_4:42142681-42142700 Msa0163800:CDS 20.0%
!!! TAGTTTGAATAGGTTGATAA+GGG + chr1_4:42140895-42140914 None:intergenic 25.0%
! GTTTCATTCAAATAGTTAGA+GGG + chr1_4:42140989-42141008 None:intergenic 25.0%
! AGTTTCATTCAAATAGTTAG+AGG + chr1_4:42140990-42141009 None:intergenic 25.0%
! GTTTCATTTGAGTAAAAACA+AGG + chr1_4:42141046-42141065 None:intergenic 25.0%
!!! ATCATTTTGAGAAGAAAAAG+TGG + chr1_4:42141107-42141126 None:intergenic 25.0%
!!! ACTTTTTCTTCTCAAAATGA+TGG - chr1_4:42141106-42141125 Msa0163800:intron 25.0%
! AAACTCATCCACAAAAAAAT+TGG + chr1_4:42141260-42141279 None:intergenic 25.0%
!!! ATTTTTTTTGAAGCAGCAAA+GGG + chr1_4:42141322-42141341 None:intergenic 25.0%
!!! AATTTTTTTTGAAGCAGCAA+AGG + chr1_4:42141323-42141342 None:intergenic 25.0%
! AGAAAAACATCAAATTCACT+TGG + chr1_4:42141679-42141698 None:intergenic 25.0%
! TTGTTTGTTCATTGCATATT+TGG - chr1_4:42141774-42141793 Msa0163800:intron 25.0%
! TAATGCTATTGTTGTAGATT+TGG - chr1_4:42141928-42141947 Msa0163800:intron 25.0%
! AAAGACAAAATTGAGACAAT+AGG + chr1_4:42141994-42142013 None:intergenic 25.0%
! TGTTAGGATTATGAAAATGT+TGG - chr1_4:42142080-42142099 Msa0163800:intron 25.0%
!!! CAATAAACAAGAGAGTATTT+AGG - chr1_4:42142200-42142219 Msa0163800:CDS 25.0%
! TTGATTATTTCAGGAAGAAA+AGG - chr1_4:42142690-42142709 Msa0163800:CDS 25.0%
! ATTATTATGATTGCTCAATG+TGG + chr1_4:42142818-42142837 None:intergenic 25.0%
!!! TAGTTTGAATAGGTTGATAA+GGG + chr1_4:42140895-42140914 None:intergenic 25.0%
! GTTTCATTCAAATAGTTAGA+GGG + chr1_4:42140989-42141008 None:intergenic 25.0%
! AGTTTCATTCAAATAGTTAG+AGG + chr1_4:42140990-42141009 None:intergenic 25.0%
! GTTTCATTTGAGTAAAAACA+AGG + chr1_4:42141046-42141065 None:intergenic 25.0%
!!! ATCATTTTGAGAAGAAAAAG+TGG + chr1_4:42141107-42141126 None:intergenic 25.0%
!!! ACTTTTTCTTCTCAAAATGA+TGG - chr1_4:42141106-42141125 Msa0163800:intron 25.0%
! AAACTCATCCACAAAAAAAT+TGG + chr1_4:42141260-42141279 None:intergenic 25.0%
!!! ATTTTTTTTGAAGCAGCAAA+GGG + chr1_4:42141322-42141341 None:intergenic 25.0%
!!! AATTTTTTTTGAAGCAGCAA+AGG + chr1_4:42141323-42141342 None:intergenic 25.0%
! AGAAAAACATCAAATTCACT+TGG + chr1_4:42141679-42141698 None:intergenic 25.0%
! TTGTTTGTTCATTGCATATT+TGG - chr1_4:42141774-42141793 Msa0163800:intron 25.0%
! TAATGCTATTGTTGTAGATT+TGG - chr1_4:42141928-42141947 Msa0163800:intron 25.0%
! AAAGACAAAATTGAGACAAT+AGG + chr1_4:42141994-42142013 None:intergenic 25.0%
! TGTTAGGATTATGAAAATGT+TGG - chr1_4:42142080-42142099 Msa0163800:intron 25.0%
!!! CAATAAACAAGAGAGTATTT+AGG - chr1_4:42142200-42142219 Msa0163800:CDS 25.0%
! TTGATTATTTCAGGAAGAAA+AGG - chr1_4:42142690-42142709 Msa0163800:CDS 25.0%
! ATTATTATGATTGCTCAATG+TGG + chr1_4:42142818-42142837 None:intergenic 25.0%
!! GTAGTTTGAATAGGTTGATA+AGG + chr1_4:42140896-42140915 None:intergenic 30.0%
!! AAGTTGTTGGTAGTTTGAAT+AGG + chr1_4:42140905-42140924 None:intergenic 30.0%
AGTAGTGATTTCTAAGTTGT+TGG + chr1_4:42140918-42140937 None:intergenic 30.0%
TCAAATAGTTAGAGGGAAAA+TGG + chr1_4:42140982-42141001 None:intergenic 30.0%
! GAATGAAATTTTCCCAAATG+AGG - chr1_4:42141141-42141160 Msa0163800:intron 30.0%
! TTTTGGACATGTAAGGTAAT+TGG + chr1_4:42141176-42141195 None:intergenic 30.0%
!!! GAACTCAACCAATTTTTTTG+TGG - chr1_4:42141249-42141268 Msa0163800:intron 30.0%
ACTTATGGAAACATTTAGCA+AGG - chr1_4:42141552-42141571 Msa0163800:intron 30.0%
TTATTAGAGCTCATAGTTGA+AGG - chr1_4:42141614-42141633 Msa0163800:intron 30.0%
ACTCAAATGATTACAAGCAA+AGG + chr1_4:42141816-42141835 None:intergenic 30.0%
!! AAATGACGGATTGAATTGTT+AGG - chr1_4:42142064-42142083 Msa0163800:intron 30.0%
CTATGTAACTGTTTAACACA+AGG - chr1_4:42142251-42142270 Msa0163800:CDS 30.0%
! AGTTTTTGTAGATCTTCACT+TGG + chr1_4:42142724-42142743 None:intergenic 30.0%
TACAGTTTCTCTTGAACAAA+TGG + chr1_4:42142757-42142776 None:intergenic 30.0%
ATCAAAACCTACCATTCTTA+AGG - chr1_4:42142843-42142862 Msa0163800:CDS 30.0%
TCAAAACCTACCATTCTTAA+GGG - chr1_4:42142844-42142863 Msa0163800:CDS 30.0%
!! GTAGTTTGAATAGGTTGATA+AGG + chr1_4:42140896-42140915 None:intergenic 30.0%
!! AAGTTGTTGGTAGTTTGAAT+AGG + chr1_4:42140905-42140924 None:intergenic 30.0%
AGTAGTGATTTCTAAGTTGT+TGG + chr1_4:42140918-42140937 None:intergenic 30.0%
TCAAATAGTTAGAGGGAAAA+TGG + chr1_4:42140982-42141001 None:intergenic 30.0%
! GAATGAAATTTTCCCAAATG+AGG - chr1_4:42141141-42141160 Msa0163800:intron 30.0%
! TTTTGGACATGTAAGGTAAT+TGG + chr1_4:42141176-42141195 None:intergenic 30.0%
!!! GAACTCAACCAATTTTTTTG+TGG - chr1_4:42141249-42141268 Msa0163800:intron 30.0%
ACTTATGGAAACATTTAGCA+AGG - chr1_4:42141552-42141571 Msa0163800:intron 30.0%
TTATTAGAGCTCATAGTTGA+AGG - chr1_4:42141614-42141633 Msa0163800:intron 30.0%
ACTCAAATGATTACAAGCAA+AGG + chr1_4:42141816-42141835 None:intergenic 30.0%
!! AAATGACGGATTGAATTGTT+AGG - chr1_4:42142064-42142083 Msa0163800:intron 30.0%
CTATGTAACTGTTTAACACA+AGG - chr1_4:42142251-42142270 Msa0163800:CDS 30.0%
! AGTTTTTGTAGATCTTCACT+TGG + chr1_4:42142724-42142743 None:intergenic 30.0%
TACAGTTTCTCTTGAACAAA+TGG + chr1_4:42142757-42142776 None:intergenic 30.0%
ATCAAAACCTACCATTCTTA+AGG - chr1_4:42142843-42142862 Msa0163800:CDS 30.0%
TCAAAACCTACCATTCTTAA+GGG - chr1_4:42142844-42142863 Msa0163800:CDS 30.0%
AATAGTTAGAGGGAAAATGG+TGG + chr1_4:42140979-42140998 None:intergenic 35.0%
CAAGTAGTTGATCTTGACAA+GGG + chr1_4:42141078-42141097 None:intergenic 35.0%
ACAAGTAGTTGATCTTGACA+AGG + chr1_4:42141079-42141098 None:intergenic 35.0%
TGGTGAAATCTTCCTCATTT+GGG + chr1_4:42141156-42141175 None:intergenic 35.0%
TTGGTGAAATCTTCCTCATT+TGG + chr1_4:42141157-42141176 None:intergenic 35.0%
AAGTTTCTTCAAGCTCAAGT+TGG - chr1_4:42141520-42141539 Msa0163800:intron 35.0%
AGTTGGAATGCTTCAACTTA+TGG - chr1_4:42141537-42141556 Msa0163800:intron 35.0%
ATTGAGACAATAGGTAGTCA+TGG + chr1_4:42141985-42142004 None:intergenic 35.0%
! ATACCATCTTTTACTCCATG+AGG - chr1_4:42142029-42142048 Msa0163800:intron 35.0%
ATTCCTCATGGAGTAAAAGA+TGG + chr1_4:42142035-42142054 None:intergenic 35.0%
AGGAAACGGTTAATAAGAAG+AGG - chr1_4:42142170-42142189 Msa0163800:intron 35.0%
CGGTTAATAAGAAGAGGAAA+TGG - chr1_4:42142176-42142195 Msa0163800:intron 35.0%
! TTTTGTAGATCTTCACTTGG+AGG + chr1_4:42142721-42142740 None:intergenic 35.0%
! AATGTGGTGACAAACTCTTT+TGG + chr1_4:42142802-42142821 None:intergenic 35.0%
AATAGTTAGAGGGAAAATGG+TGG + chr1_4:42140979-42140998 None:intergenic 35.0%
CAAGTAGTTGATCTTGACAA+GGG + chr1_4:42141078-42141097 None:intergenic 35.0%
ACAAGTAGTTGATCTTGACA+AGG + chr1_4:42141079-42141098 None:intergenic 35.0%
TGGTGAAATCTTCCTCATTT+GGG + chr1_4:42141156-42141175 None:intergenic 35.0%
TTGGTGAAATCTTCCTCATT+TGG + chr1_4:42141157-42141176 None:intergenic 35.0%
AAGTTTCTTCAAGCTCAAGT+TGG - chr1_4:42141520-42141539 Msa0163800:intron 35.0%
AGTTGGAATGCTTCAACTTA+TGG - chr1_4:42141537-42141556 Msa0163800:intron 35.0%
ATTGAGACAATAGGTAGTCA+TGG + chr1_4:42141985-42142004 None:intergenic 35.0%
! ATACCATCTTTTACTCCATG+AGG - chr1_4:42142029-42142048 Msa0163800:intron 35.0%
ATTCCTCATGGAGTAAAAGA+TGG + chr1_4:42142035-42142054 None:intergenic 35.0%
AGGAAACGGTTAATAAGAAG+AGG - chr1_4:42142170-42142189 Msa0163800:intron 35.0%
CGGTTAATAAGAAGAGGAAA+TGG - chr1_4:42142176-42142195 Msa0163800:intron 35.0%
! TTTTGTAGATCTTCACTTGG+AGG + chr1_4:42142721-42142740 None:intergenic 35.0%
! AATGTGGTGACAAACTCTTT+TGG + chr1_4:42142802-42142821 None:intergenic 35.0%
GTTGATCTTGACAAGGGTTA+GGG + chr1_4:42141072-42141091 None:intergenic 40.0%
AGTTGATCTTGACAAGGGTT+AGG + chr1_4:42141073-42141092 None:intergenic 40.0%
!! TTTGGCGTTTTGGACATGTA+AGG + chr1_4:42141183-42141202 None:intergenic 40.0%
! TAGCATAGCTTTTGGCGTTT+TGG + chr1_4:42141193-42141212 None:intergenic 40.0%
GAGTTCAAAAGCTCTTGTTG+TGG + chr1_4:42141235-42141254 None:intergenic 40.0%
CAAAGGAAGCAAAAGGGTTA+GGG + chr1_4:42141291-42141310 None:intergenic 40.0%
TCAAAGGAAGCAAAAGGGTT+AGG + chr1_4:42141292-42141311 None:intergenic 40.0%
! TAACCCTTTTGCTTCCTTTG+AGG - chr1_4:42141291-42141310 Msa0163800:intron 40.0%
GTGAGAAGAAAGTTGCTGAA+AGG - chr1_4:42141359-42141378 Msa0163800:intron 40.0%
ATCTCTGCTGTGTTCATCTT+TGG + chr1_4:42141475-42141494 None:intergenic 40.0%
GCAATGAACAAACAAACCCT+AGG + chr1_4:42141769-42141788 None:intergenic 40.0%
! GAGAGAGTGTGTTAAGGAAA+CGG - chr1_4:42142156-42142175 Msa0163800:intron 40.0%
CAAGTGTCTATCAAATGAGC+TGG - chr1_4:42142228-42142247 Msa0163800:CDS 40.0%
TTCAGACCCTTAAGAATGGT+AGG + chr1_4:42142853-42142872 None:intergenic 40.0%
CTGTTTCAGACCCTTAAGAA+TGG + chr1_4:42142857-42142876 None:intergenic 40.0%
GTTGATCTTGACAAGGGTTA+GGG + chr1_4:42141072-42141091 None:intergenic 40.0%
AGTTGATCTTGACAAGGGTT+AGG + chr1_4:42141073-42141092 None:intergenic 40.0%
!! TTTGGCGTTTTGGACATGTA+AGG + chr1_4:42141183-42141202 None:intergenic 40.0%
! TAGCATAGCTTTTGGCGTTT+TGG + chr1_4:42141193-42141212 None:intergenic 40.0%
GAGTTCAAAAGCTCTTGTTG+TGG + chr1_4:42141235-42141254 None:intergenic 40.0%
CAAAGGAAGCAAAAGGGTTA+GGG + chr1_4:42141291-42141310 None:intergenic 40.0%
TCAAAGGAAGCAAAAGGGTT+AGG + chr1_4:42141292-42141311 None:intergenic 40.0%
! TAACCCTTTTGCTTCCTTTG+AGG - chr1_4:42141291-42141310 Msa0163800:intron 40.0%
GTGAGAAGAAAGTTGCTGAA+AGG - chr1_4:42141359-42141378 Msa0163800:intron 40.0%
ATCTCTGCTGTGTTCATCTT+TGG + chr1_4:42141475-42141494 None:intergenic 40.0%
GCAATGAACAAACAAACCCT+AGG + chr1_4:42141769-42141788 None:intergenic 40.0%
! GAGAGAGTGTGTTAAGGAAA+CGG - chr1_4:42142156-42142175 Msa0163800:intron 40.0%
CAAGTGTCTATCAAATGAGC+TGG - chr1_4:42142228-42142247 Msa0163800:CDS 40.0%
TTCAGACCCTTAAGAATGGT+AGG + chr1_4:42142853-42142872 None:intergenic 40.0%
CTGTTTCAGACCCTTAAGAA+TGG + chr1_4:42142857-42142876 None:intergenic 40.0%
! TCTCCTCGTAGCATAGCTTT+TGG + chr1_4:42141201-42141220 None:intergenic 45.0%
ATGCTACGAGGAGAAAAGAG+AGG - chr1_4:42141207-42141226 Msa0163800:intron 45.0%
CTGCCTCAAAGGAAGCAAAA+GGG + chr1_4:42141297-42141316 None:intergenic 45.0%
TCTGCCTCAAAGGAAGCAAA+AGG + chr1_4:42141298-42141317 None:intergenic 45.0%
ACTGAGAAGACACAAGAGCT+TGG - chr1_4:42141430-42141449 Msa0163800:intron 45.0%
CTGAGAAGACACAAGAGCTT+GGG - chr1_4:42141431-42141450 Msa0163800:intron 45.0%
GACACAAGAGCTTGGGAAAT+TGG - chr1_4:42141438-42141457 Msa0163800:intron 45.0%
! GAGAGAGAGAGAGTGTGTTA+AGG - chr1_4:42142150-42142169 Msa0163800:intron 45.0%
TGTAGATCTTCACTTGGAGG+AGG + chr1_4:42142718-42142737 None:intergenic 45.0%
! TCTCCTCGTAGCATAGCTTT+TGG + chr1_4:42141201-42141220 None:intergenic 45.0%
ATGCTACGAGGAGAAAAGAG+AGG - chr1_4:42141207-42141226 Msa0163800:intron 45.0%
CTGCCTCAAAGGAAGCAAAA+GGG + chr1_4:42141297-42141316 None:intergenic 45.0%
TCTGCCTCAAAGGAAGCAAA+AGG + chr1_4:42141298-42141317 None:intergenic 45.0%
ACTGAGAAGACACAAGAGCT+TGG - chr1_4:42141430-42141449 Msa0163800:intron 45.0%
CTGAGAAGACACAAGAGCTT+GGG - chr1_4:42141431-42141450 Msa0163800:intron 45.0%
GACACAAGAGCTTGGGAAAT+TGG - chr1_4:42141438-42141457 Msa0163800:intron 45.0%
! GAGAGAGAGAGAGTGTGTTA+AGG - chr1_4:42142150-42142169 Msa0163800:intron 45.0%
TGTAGATCTTCACTTGGAGG+AGG + chr1_4:42142718-42142737 None:intergenic 45.0%
ACGCCAAAAGCTATGCTACG+AGG - chr1_4:42141195-42141214 Msa0163800:intron 50.0%
AGCAAAGGGTTCTGCCTCAA+AGG + chr1_4:42141308-42141327 None:intergenic 50.0%
!! TATCTCGTCGCAGAGTATCG+CGG - chr1_4:42141384-42141403 Msa0163800:intron 50.0%
! GAGCTTGGGAAATTGGTTCC+TGG - chr1_4:42141445-42141464 Msa0163800:intron 50.0%
! CTTGGGAAATTGGTTCCTGG+TGG - chr1_4:42141448-42141467 Msa0163800:intron 50.0%
GTGTTCATCTTTGGTCCACC+AGG + chr1_4:42141466-42141485 None:intergenic 50.0%
! AAGGGTCTGAAACAGCTTGC+TGG - chr1_4:42142862-42142881 Msa0163800:CDS 50.0%
! AGGGTCTGAAACAGCTTGCT+GGG - chr1_4:42142863-42142882 Msa0163800:CDS 50.0%
ACGCCAAAAGCTATGCTACG+AGG - chr1_4:42141195-42141214 Msa0163800:intron 50.0%
AGCAAAGGGTTCTGCCTCAA+AGG + chr1_4:42141308-42141327 None:intergenic 50.0%
!! TATCTCGTCGCAGAGTATCG+CGG - chr1_4:42141384-42141403 Msa0163800:intron 50.0%
! GAGCTTGGGAAATTGGTTCC+TGG - chr1_4:42141445-42141464 Msa0163800:intron 50.0%
! CTTGGGAAATTGGTTCCTGG+TGG - chr1_4:42141448-42141467 Msa0163800:intron 50.0%
GTGTTCATCTTTGGTCCACC+AGG + chr1_4:42141466-42141485 None:intergenic 50.0%
! AAGGGTCTGAAACAGCTTGC+TGG - chr1_4:42142862-42142881 Msa0163800:CDS 50.0%
! AGGGTCTGAAACAGCTTGCT+GGG - chr1_4:42142863-42142882 Msa0163800:CDS 50.0%
!! AGAGTATCGCGGCGAGAGAA+AGG - chr1_4:42141395-42141414 Msa0163800:intron 55.0%
!! AGAGTATCGCGGCGAGAGAA+AGG - chr1_4:42141395-42141414 Msa0163800:intron 55.0%
TCGCGGCGAGAGAAAGGAGA+AGG - chr1_4:42141401-42141420 Msa0163800:intron 60.0%
TCGCGGCGAGAGAAAGGAGA+AGG - chr1_4:42141401-42141420 Msa0163800:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 42140854 42142918 42140854 ID=Msa0163800;Name=Msa0163800
chr1_4 mRNA 42140854 42142918 42140854 ID=Msa0163800-mRNA-1;Parent=Msa0163800;Name=Msa0163800-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|2|0|311
chr1_4 exon 42142199 42142918 42142199 ID=Msa0163800-mRNA-1:exon:12217;Parent=Msa0163800-mRNA-1
chr1_4 exon 42140854 42141069 42140854 ID=Msa0163800-mRNA-1:exon:12216;Parent=Msa0163800-mRNA-1
chr1_4 CDS 42142199 42142918 42142199 ID=Msa0163800-mRNA-1:cds;Parent=Msa0163800-mRNA-1
chr1_4 CDS 42140854 42141069 42140854 ID=Msa0163800-mRNA-1:cds;Parent=Msa0163800-mRNA-1
Gene Sequence

>Msa0163800

ATGGGAGCACTTAGCACATACTTCAACTATAATTGTTACCCTTATCAACCTATTCAAACTACCAACAACTTAGAAATCACTACTTTTCAACATCATGAACAAGAACAATTTGTGCAACAAAACCACCATTTTCCCTCTAACTATTTGAATGAAACTTCTCTTTTAGATGAAAGCTTCATTTTTCAAACTCCTTGTTTTTACTCAAATGAAACTTACCCTAACCCTTGTCAAGATCAACTACTTGTTGACTCCACTTTTTCTTCTCAAAATGATGGCTTTGTTTCAATGAATGAAATTTTCCCAAATGAGGAAGATTTCACCAATTACCTTACATGTCCAAAACGCCAAAAGCTATGCTACGAGGAGAAAAGAGAGGAACCACAACAAGAGCTTTTGAACTCAACCAATTTTTTTGTGGATGAGTTTATGACTAACCCTAACCCTTTTGCTTCCTTTGAGGCAGAACCCTTTGCTGCTTCAAAAAAAATTGTTGATGTTGTTCAATGTGAGAAGAAAGTTGCTGAAAGGACTATCTCGTCGCAGAGTATCGCGGCGAGAGAAAGGAGAAGGAAGATAACTGAGAAGACACAAGAGCTTGGGAAATTGGTTCCTGGTGGACCAAAGATGAACACAGCAGAGATGCTTAATGCTGCTGCTAATTATGTCAAGTTTCTTCAAGCTCAAGTTGGAATGCTTCAACTTATGGAAACATTTAGCAAGGAAGAAAAGGAACCTCCTCCAAGTGAAGATCTACAAAAACTACTTCTTTCTCCATTTGTTCAAGAGAAACTGTATTCAGAAGAAAAGTGCTTTGTTCCAAAAGAGTTTGTCACCACATTGAGCAATCATAATAATGTTCAATCAAAACCTACCATTCTTAAGGGTCTGAAACAGCTTGCTGGGACTGAGATTGAGAAGAAACCAAAGCAAGAATAG
Protein sequence

>Msa0163800

MGALSTYFNYNCYPYQPIQTTNNLEITTFQHHEQEQFVQQNHHFPSNYLNETSLLDESFIFQTPCFYSNETYPNPCQDQLLVDSTFSSQNDGFVSMNEIFPNEEDFTNYLTCPKRQKLCYEEKREEPQQELLNSTNFFVDEFMTNPNPFASFEAEPFAASKKIVDVVQCEKKVAERTISSQSIAARERRRKITEKTQELGKLVPGGPKMNTAEMLNAAANYVKFLQAQVGMLQLMETFSKEEKEPPPSEDLQKLLLSPFVQEKLYSEEKCFVPKEFVTTLSNHNNVQSKPTILKGLKQLAGTEIEKKPKQE*