AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0165200

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0165200 MtrunA17_Chr1g0164761 87.759 580 51 3 1 579 1 561 0.0 1043
Msa0165200 MtrunA17_Chr1g0164731 76.049 572 109 3 2 571 39 584 0.0 869
Msa0165200 MtrunA17_Chr5g0410721 52.008 498 233 2 2 494 83 579 0.0 549
Msa0165200 MtrunA17_Chr3g0116441 38.333 360 186 8 8 346 18 362 2.41e-67 238
Msa0165200 MtrunA17_Chr8g0380981 36.010 386 170 8 7 325 42 417 5.78e-57 206
Msa0165200 MtrunA17_Chr7g0214951 77.500 120 9 2 181 299 99 201 1.64e-56 189
Msa0165200 MtrunA17_Chr1g0189211 29.981 537 283 16 6 463 148 670 2.35e-54 199
Msa0165200 MtrunA17_Chr1g0199511 45.745 188 98 1 152 339 291 474 3.24e-53 196
Msa0165200 MtrunA17_Chr5g0397501 34.545 385 200 13 109 464 323 684 3.67e-51 190
Msa0165200 MtrunA17_Chr2g0303101 32.506 403 199 9 7 344 51 445 6.81e-51 189
Msa0165200 MtrunA17_Chr7g0261401 31.193 327 178 12 156 456 338 643 5.02e-30 125
Msa0165200 MtrunA17_Chr7g0214941 75.342 73 17 1 470 541 2 74 2.57e-25 99.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0165200 AT5G46910.2 54.140 471 215 1 2 472 97 566 0.0 540
Msa0165200 AT5G46910.1 54.140 471 215 1 2 472 97 566 0.0 539
Msa0165200 AT2G34880.1 30.741 540 285 15 4 471 57 579 5.71e-59 211
Msa0165200 AT3G48430.1 35.054 368 197 6 9 339 21 383 2.28e-58 211
Msa0165200 AT1G63490.1 36.267 375 170 11 94 431 53 395 5.26e-57 207
Msa0165200 AT1G63490.2 36.267 375 170 11 94 431 306 648 7.05e-57 207
Msa0165200 AT1G63490.4 36.267 375 170 11 94 431 306 648 7.12e-57 207
Msa0165200 AT1G30810.3 29.664 536 289 13 7 467 58 580 3.52e-56 203
Msa0165200 AT1G30810.2 29.664 536 289 13 7 467 58 580 3.52e-56 203
Msa0165200 AT1G30810.1 29.664 536 289 13 7 467 58 580 3.52e-56 203
Msa0165200 AT4G20400.1 27.362 614 327 19 5 529 53 636 7.13e-55 200
Msa0165200 AT4G20400.2 27.907 602 317 19 16 529 7 579 4.37e-52 192
Msa0165200 AT5G04240.1 46.277 188 97 1 152 339 259 442 4.92e-52 193
Msa0165200 AT1G08620.3 40.000 240 124 4 117 343 327 559 3.25e-45 172
Msa0165200 AT1G08620.2 40.000 240 124 4 117 343 313 545 3.84e-45 172
Msa0165200 AT1G08620.4 40.000 240 124 4 117 343 313 545 3.84e-45 172
Msa0165200 AT1G08620.1 40.000 240 124 4 117 343 313 545 3.84e-45 172
Msa0165200 AT2G38950.1 24.881 631 367 20 2 550 102 707 6.10e-34 137
Msa0165200 AT1G63490.3 34.848 198 86 5 254 431 15 189 2.83e-24 108

Find 87 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACAATTGTACAGCCTCTT+AGG 0.195974 1_4:-45390598 Msa0165200:CDS
GATGCATTCATTTATGTTAT+AGG 0.215836 1_4:+45388346 None:intergenic
TAGGAGAATATATTTGTTAA+AGG 0.243804 1_4:+45388365 None:intergenic
AATCCAGCATGATATGAATT+TGG 0.254423 1_4:+45389501 None:intergenic
TTCTTTACCACGCAATCTAT+TGG 0.264442 1_4:+45390389 None:intergenic
TCCTTCAATATCTTCCCTTT+TGG 0.303945 1_4:-45387910 Msa0165200:CDS
ACTGAAAACAGAATAATAAT+TGG 0.315714 1_4:-45388148 Msa0165200:intron
AGTTCCTCATATGGAATTAT+AGG 0.316333 1_4:+45388885 None:intergenic
GAGAAAGGAACAGTTGAGTA+TGG 0.324983 1_4:-45390312 Msa0165200:CDS
ATAAAGGACTCGTTGTCTTT+TGG 0.342530 1_4:+45387971 None:intergenic
TTACAAAGAAGTTCCTCATA+TGG 0.344918 1_4:+45388876 None:intergenic
TTACCATGGAAAAGGCAAAT+TGG 0.359872 1_4:+45388600 None:intergenic
AGCTCTTCAAATACATCATT+AGG 0.370354 1_4:-45388696 Msa0165200:CDS
AACAAGCAATTTCAATTTGA+AGG 0.380018 1_4:-45390238 Msa0165200:intron
TAAACTTTGCTATTGGTGAT+TGG 0.380389 1_4:-45388959 Msa0165200:CDS
TGACATGCAGCCTTTGCAAT+AGG 0.385933 1_4:-45388668 Msa0165200:CDS
CTCCATTGCGTTTAGTTGAC+AGG 0.390855 1_4:-45390031 Msa0165200:CDS
GACAAAAGAAGCAGGATTAG+AGG 0.392437 1_4:+45390652 None:intergenic
GGAAGATATTGAAGGAACTT+TGG 0.410410 1_4:+45387917 None:intergenic
TTGTCTTAACGGAAGAGAAA+AGG 0.418102 1_4:-45390634 Msa0165200:CDS
CTGATCCTATGCTTTACATT+GGG 0.424948 1_4:-45389907 Msa0165200:CDS
TGCTGCCAAAAGTCTTCAAC+AGG 0.428587 1_4:-45388411 Msa0165200:CDS
CACAATTGTACAGCCTCTTA+GGG 0.435355 1_4:-45390597 Msa0165200:CDS
CTTGTTCCAAGCTTATCATC+AGG 0.440827 1_4:+45390255 None:intergenic
GACATGCAGCCTTTGCAATA+GGG 0.448222 1_4:-45388667 Msa0165200:CDS
GAAGCTGTAAACTTTGCTAT+TGG 0.453482 1_4:-45388966 Msa0165200:CDS
TTGGTGATTGGTTTCCACTC+GGG 0.456519 1_4:-45388947 Msa0165200:CDS
TTTGATTCGAAAACCCTAAG+AGG 0.457545 1_4:+45390584 None:intergenic
GCTACCTATAATTCCATATG+AGG 0.458631 1_4:-45388889 Msa0165200:CDS
TAACTTGAGAAAACATGAAT+CGG 0.462602 1_4:-45387949 Msa0165200:CDS
TGTCTTAACGGAAGAGAAAA+GGG 0.470673 1_4:-45390633 Msa0165200:CDS
TGCTCAAACTCTTGTTCTGA+TGG 0.476367 1_4:+45391149 None:intergenic
TACAAGGCGGTGCAAAAGCC+AGG 0.481676 1_4:-45389543 Msa0165200:CDS
TTAAAAGAACATGGAGAGAA+TGG 0.487266 1_4:-45389639 Msa0165200:CDS
ACATAATGTGCCGGTTTACA+AGG 0.487938 1_4:-45389559 Msa0165200:CDS
ATACCAAATTTAGACGCCTC+GGG 0.488483 1_4:+45391098 None:intergenic
TTGAAGATCACTATTTGTAC+AGG 0.488775 1_4:-45389856 Msa0165200:intron
ATTTGAAGCTCTTGCAAACA+AGG 0.496352 1_4:-45390418 Msa0165200:CDS
TTTCCAAATTCATATCATGC+TGG 0.496493 1_4:-45389504 Msa0165200:CDS
TGCCACAAAATTGTTTATGC+GGG 0.497644 1_4:-45388481 Msa0165200:CDS
ATCATCAGGATCACTTGAAA+AGG 0.501772 1_4:+45390269 None:intergenic
GGTGCAAATAAAACTTGGTA+TGG 0.503293 1_4:-45389729 Msa0165200:CDS
ATTGGTGATTGGTTTCCACT+CGG 0.503752 1_4:-45388948 Msa0165200:CDS
ACGAGGTCTTACCATGGAAA+AGG 0.511206 1_4:+45388592 None:intergenic
GAAATAGTACATGGAGAGAA+AGG 0.511259 1_4:-45390327 Msa0165200:CDS
CAAAATCAAACTTGAAGATA+CGG 0.514277 1_4:-45388811 Msa0165200:CDS
ACTGATCCTATGCTTTACAT+TGG 0.515442 1_4:-45389908 Msa0165200:CDS
AGTGATCCTGATGATAAGCT+TGG 0.516084 1_4:-45390261 Msa0165200:CDS
TGCTATTCAGTATGTTTGCA+TGG 0.518171 1_4:-45389883 Msa0165200:CDS
TGGTGATTGGTTTCCACTCG+GGG 0.521993 1_4:-45388946 Msa0165200:CDS
TCCGTTAAGACAAAAGAAGC+AGG 0.523048 1_4:+45390644 None:intergenic
GAGAACATACTGTATTTAAG+AGG 0.523058 1_4:-45388457 Msa0165200:CDS
TAAGACATCGCTATCGCAGT+TGG 0.526345 1_4:-45388739 Msa0165200:CDS
AATTGAGAGGATGCAGAACT+AGG 0.531709 1_4:+45389702 None:intergenic
ACTCAGGTGCAAATAAAACT+TGG 0.537060 1_4:-45389734 Msa0165200:CDS
TTATGTTCTAACAAGACATC+AGG 0.538881 1_4:+45389576 None:intergenic
CTTGTTAGAACATAATGTGC+CGG 0.547471 1_4:-45389568 Msa0165200:CDS
AATGGAGACTGTGGCAGCCG+CGG 0.554838 1_4:+45390047 None:intergenic
GCAGGATTAGAGGCAGCGAT+TGG 0.560174 1_4:+45390662 None:intergenic
AAGGTGACGACAAACTCTCC+TGG 0.561754 1_4:+45389525 None:intergenic
CAAGACATCAGGAGAGAACA+TGG 0.563327 1_4:+45389587 None:intergenic
GTGAGCTTGATGTTGTTCGC+AGG 0.564971 1_4:+45388059 None:intergenic
CATACCAAATTTAGACGCCT+CGG 0.566207 1_4:+45391097 None:intergenic
TGTTCTGATGGATAATACGT+TGG 0.567602 1_4:+45391161 None:intergenic
GCCAAAAGGGAAGATATTGA+AGG 0.571210 1_4:+45387909 None:intergenic
TTCCTGTCAACTAAACGCAA+TGG 0.577093 1_4:+45390029 None:intergenic
AGAAGAACTGTTAACTCACT+AGG 0.579133 1_4:-45388107 Msa0165200:CDS
CATAACAATCCCTATTGCAA+AGG 0.580709 1_4:+45388658 None:intergenic
GTGCCACAAAATTGTTTATG+CGG 0.580719 1_4:-45388482 Msa0165200:CDS
TTGCTAGCTGCGGCCCCGAG+TGG 0.581206 1_4:+45388933 None:intergenic
ATTTAAGAGGAATGACATGT+TGG 0.584034 1_4:-45388444 Msa0165200:CDS
TTCTGGCACGAAATAGTACA+TGG 0.584729 1_4:-45390336 Msa0165200:CDS
TATCATGCTGGATTTAGTCA+TGG 0.587724 1_4:-45389492 Msa0165200:intron
ATTTGTTAAAGGTAACGCGT+TGG 0.609530 1_4:+45388376 None:intergenic
AGCATCCCAATGTAAAGCAT+AGG 0.618659 1_4:+45389902 None:intergenic
TACAACTCCTCAAAAGCTAG+AGG 0.624733 1_4:-45388837 Msa0165200:CDS
TGAATAACGTTTGCTAGCTG+CGG 0.626370 1_4:+45388923 None:intergenic
AGCATAAATTACCATCACTC+AGG 0.628951 1_4:-45389750 Msa0165200:CDS
ACAGAATAATAATTGGCATG+AGG 0.629929 1_4:-45388141 Msa0165200:intron
TACCAAATTTAGACGCCTCG+GGG 0.634434 1_4:+45391099 None:intergenic
AGCATGATATGAATTTGGAA+AGG 0.636839 1_4:+45389506 None:intergenic
AACAAAGTCTTAAAAGAACA+TGG 0.646276 1_4:-45389648 Msa0165200:CDS
TGAATGCATCAAAGAACACA+AGG 0.658928 1_4:-45388333 Msa0165200:intron
ACCAAATTTAGACGCCTCGG+GGG 0.671194 1_4:+45391100 None:intergenic
ACTAAACGCAATGGAGACTG+TGG 0.698121 1_4:+45390038 None:intergenic
TAATGTGCCGGTTTACAAGG+CGG 0.701604 1_4:-45389556 Msa0165200:CDS
TGAGTATGGAGTCAATGTTG+AGG 0.703101 1_4:-45390298 Msa0165200:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTTTATTCAAAATTTC+AGG - chr1_4:45389136-45389155 Msa0165200:intron 10.0%
!!! TAATTTTATTCAAAATTTCA+GGG - chr1_4:45389137-45389156 Msa0165200:intron 10.0%
!!! TATTGTTTTCTTTTCATTTT+AGG - chr1_4:45388013-45388032 Msa0165200:CDS 15.0%
!! ATATTTGATCTTAATATTTC+AGG - chr1_4:45388368-45388387 Msa0165200:CDS 15.0%
!!! AACAACTTTTAATTCATATT+AGG + chr1_4:45389097-45389116 None:intergenic 15.0%
!! AGAACGATATAATCTAATAT+AGG - chr1_4:45388276-45388295 Msa0165200:intron 20.0%
!!! TAACTTTTTCTAAGAGAAAA+AGG - chr1_4:45388525-45388544 Msa0165200:intron 20.0%
!! TGATGAAAAATTAACTAACT+TGG - chr1_4:45388591-45388610 Msa0165200:intron 20.0%
!!! CTTATATTTTACGAATAAGT+AGG - chr1_4:45389690-45389709 Msa0165200:CDS 20.0%
!!! AATCTGTTTATTTTTGTACA+AGG - chr1_4:45389862-45389881 Msa0165200:CDS 20.0%
!!! TTTTTTTCCATAATAATACC+TGG - chr1_4:45389942-45389961 Msa0165200:intron 20.0%
!!! ACATAAGTATTTAAAATAGC+AGG + chr1_4:45389981-45390000 None:intergenic 20.0%
!!! TAGGAGAATATATTTGTTAA+AGG + chr1_4:45390710-45390729 None:intergenic 20.0%
!! ACTATTATAATGAAGTTTAG+AGG + chr1_4:45390808-45390827 None:intergenic 20.0%
!!! ATCTGATTGATTTTAACTTT+TGG - chr1_4:45390894-45390913 Msa0165200:intron 20.0%
!! ACTGAAAACAGAATAATAAT+TGG - chr1_4:45390924-45390943 Msa0165200:intron 20.0%
!! ATTTTCTGCAGATAAACTAA+AGG + chr1_4:45387950-45387969 None:intergenic 25.0%
! AGTAGAATTCTTACTATACT+TGG + chr1_4:45388090-45388109 None:intergenic 25.0%
!! AGATTTTACTAAAATCACAG+TGG - chr1_4:45388237-45388256 Msa0165200:intron 25.0%
! AACAAGCAATTTCAATTTGA+AGG - chr1_4:45388834-45388853 Msa0165200:CDS 25.0%
! AACAAAGTCTTAAAAGAACA+TGG - chr1_4:45389424-45389443 Msa0165200:intron 25.0%
!!! TTGTTTTTTGTGCAAGAAAT+TGG + chr1_4:45389465-45389484 None:intergenic 25.0%
!! AGGTTGATTGAATAATAGAA+AGG - chr1_4:45389714-45389733 Msa0165200:CDS 25.0%
! CTTTCTATTATTCAAGCTTA+GGG + chr1_4:45389769-45389788 None:intergenic 25.0%
!! ATCATTTTCTTGTTGAGTTT+AGG - chr1_4:45390070-45390089 Msa0165200:intron 25.0%
! CAAAATCAAACTTGAAGATA+CGG - chr1_4:45390261-45390280 Msa0165200:CDS 25.0%
!! ATGGATAAACTTGCTTATTA+TGG - chr1_4:45390552-45390571 Msa0165200:CDS 25.0%
! GATGCATTCATTTATGTTAT+AGG + chr1_4:45390729-45390748 None:intergenic 25.0%
!! CATGTTTTCTCAAGTTATAA+AGG + chr1_4:45391120-45391139 None:intergenic 25.0%
! TAACTTGAGAAAACATGAAT+CGG - chr1_4:45391123-45391142 Msa0165200:CDS 25.0%
AAAGAAAGTTGAGGGATAAA+AGG + chr1_4:45388124-45388143 None:intergenic 30.0%
! TTTATCCCTCAACTTTCTTT+TGG - chr1_4:45388124-45388143 Msa0165200:CDS 30.0%
!!! TTTGATTTTAGAATTTGCCG+CGG - chr1_4:45389008-45389027 Msa0165200:intron 30.0%
TACAAAAATTGCTTCCTTAC+TGG + chr1_4:45389073-45389092 None:intergenic 30.0%
TTGAAGATCACTATTTGTAC+AGG - chr1_4:45389216-45389235 Msa0165200:intron 30.0%
TTAAAAGAACATGGAGAGAA+TGG - chr1_4:45389433-45389452 Msa0165200:intron 30.0%
TTATGTTCTAACAAGACATC+AGG + chr1_4:45389499-45389518 None:intergenic 30.0%
AGCATGATATGAATTTGGAA+AGG + chr1_4:45389569-45389588 None:intergenic 30.0%
TTTCCAAATTCATATCATGC+TGG - chr1_4:45389568-45389587 Msa0165200:CDS 30.0%
AATCCAGCATGATATGAATT+TGG + chr1_4:45389574-45389593 None:intergenic 30.0%
! TATTTTACGAATAAGTAGGC+AGG - chr1_4:45389694-45389713 Msa0165200:CDS 30.0%
! TGAATAATAGAAAGGTACGT+AGG - chr1_4:45389722-45389741 Msa0165200:CDS 30.0%
CCTTTCTATTATTCAAGCTT+AGG + chr1_4:45389770-45389789 None:intergenic 30.0%
CCTAAGCTTGAATAATAGAA+AGG - chr1_4:45389767-45389786 Msa0165200:intron 30.0%
! TTTTCCATAATAATACCTGG+CGG - chr1_4:45389945-45389964 Msa0165200:intron 30.0%
!!! TTGAGTTTAGGTTTTAACTG+TGG - chr1_4:45390082-45390101 Msa0165200:intron 30.0%
!! TAAACTTTGCTATTGGTGAT+TGG - chr1_4:45390113-45390132 Msa0165200:intron 30.0%
AGTTCCTCATATGGAATTAT+AGG + chr1_4:45390190-45390209 None:intergenic 30.0%
TTACAAAGAAGTTCCTCATA+TGG + chr1_4:45390199-45390218 None:intergenic 30.0%
AGCTCTTCAAATACATCATT+AGG - chr1_4:45390376-45390395 Msa0165200:CDS 30.0%
GAGAACATACTGTATTTAAG+AGG - chr1_4:45390615-45390634 Msa0165200:CDS 30.0%
ATTTAAGAGGAATGACATGT+TGG - chr1_4:45390628-45390647 Msa0165200:CDS 30.0%
! TTTTTCCTGTTGAAGACTTT+TGG + chr1_4:45390669-45390688 None:intergenic 30.0%
TAACACATTGCTTTATCTTC+AGG - chr1_4:45390855-45390874 Msa0165200:intron 30.0%
ACAGAATAATAATTGGCATG+AGG - chr1_4:45390931-45390950 Msa0165200:intron 30.0%
! TGTTCTGATGGATAATACGT+TGG + chr1_4:45387914-45387933 None:intergenic 35.0%
TGGATCCAAAAGAAAGTTGA+GGG + chr1_4:45388132-45388151 None:intergenic 35.0%
ATGGATCCAAAAGAAAGTTG+AGG + chr1_4:45388133-45388152 None:intergenic 35.0%
! GCAACAGATTCTGCATTTTT+AGG - chr1_4:45388311-45388330 Msa0165200:intron 35.0%
! TCCTGCTTCTTTTGTCTTAA+CGG - chr1_4:45388427-45388446 Msa0165200:CDS 35.0%
TTGTCTTAACGGAAGAGAAA+AGG - chr1_4:45388438-45388457 Msa0165200:CDS 35.0%
TGTCTTAACGGAAGAGAAAA+GGG - chr1_4:45388439-45388458 Msa0165200:CDS 35.0%
! TTTGATTCGAAAACCCTAAG+AGG + chr1_4:45388491-45388510 None:intergenic 35.0%
! GGTTTTCGAATCAAATGAGA+TGG - chr1_4:45388496-45388515 Msa0165200:intron 35.0%
! ATTTGAAGCTCTTGCAAACA+AGG - chr1_4:45388654-45388673 Msa0165200:CDS 35.0%
TTCTTTACCACGCAATCTAT+TGG + chr1_4:45388686-45388705 None:intergenic 35.0%
GAAATAGTACATGGAGAGAA+AGG - chr1_4:45388745-45388764 Msa0165200:CDS 35.0%
ATCATCAGGATCACTTGAAA+AGG + chr1_4:45388806-45388825 None:intergenic 35.0%
TTGACAGGAAAATTCCAGTA+AGG - chr1_4:45389056-45389075 Msa0165200:intron 35.0%
ACTGATCCTATGCTTTACAT+TGG - chr1_4:45389164-45389183 Msa0165200:intron 35.0%
CTGATCCTATGCTTTACATT+GGG - chr1_4:45389165-45389184 Msa0165200:intron 35.0%
TGCTATTCAGTATGTTTGCA+TGG - chr1_4:45389189-45389208 Msa0165200:intron 35.0%
AGCATAAATTACCATCACTC+AGG - chr1_4:45389322-45389341 Msa0165200:intron 35.0%
!! TTTTATTTGCACCTGAGTGA+TGG + chr1_4:45389336-45389355 None:intergenic 35.0%
ACTCAGGTGCAAATAAAACT+TGG - chr1_4:45389338-45389357 Msa0165200:intron 35.0%
GGTGCAAATAAAACTTGGTA+TGG - chr1_4:45389343-45389362 Msa0165200:intron 35.0%
! GACAGTCTTTTCAAATTGAG+AGG + chr1_4:45389386-45389405 None:intergenic 35.0%
CTTGTTAGAACATAATGTGC+CGG - chr1_4:45389504-45389523 Msa0165200:CDS 35.0%
TATCATGCTGGATTTAGTCA+TGG - chr1_4:45389580-45389599 Msa0165200:CDS 35.0%
!! TGGTGTTATTACGTTTCTGA+TGG - chr1_4:45389661-45389680 Msa0165200:CDS 35.0%
! AGAAAGGTACGTAGGTTTAA+AGG - chr1_4:45389730-45389749 Msa0165200:CDS 35.0%
CGAGATTTGATTATGGAGTT+CGG + chr1_4:45389830-45389849 None:intergenic 35.0%
AATTCCGCCAGGTATTATTA+TGG + chr1_4:45389952-45389971 None:intergenic 35.0%
! GAAGCTGTAAACTTTGCTAT+TGG - chr1_4:45390106-45390125 Msa0165200:intron 35.0%
GCTACCTATAATTCCATATG+AGG - chr1_4:45390183-45390202 Msa0165200:intron 35.0%
CATAACAATCCCTATTGCAA+AGG + chr1_4:45390417-45390436 None:intergenic 35.0%
TTACCATGGAAAAGGCAAAT+TGG + chr1_4:45390475-45390494 None:intergenic 35.0%
GAAATACTCAGAGAATAACG+AGG + chr1_4:45390500-45390519 None:intergenic 35.0%
! TAAGCTGCTTGTGTTATTGA+TGG - chr1_4:45390533-45390552 Msa0165200:intron 35.0%
GTGCCACAAAATTGTTTATG+CGG - chr1_4:45390590-45390609 Msa0165200:CDS 35.0%
TGCCACAAAATTGTTTATGC+GGG - chr1_4:45390591-45390610 Msa0165200:CDS 35.0%
! ATTTGTTAAAGGTAACGCGT+TGG + chr1_4:45390699-45390718 None:intergenic 35.0%
TGAATGCATCAAAGAACACA+AGG - chr1_4:45390739-45390758 Msa0165200:intron 35.0%
AGAAGAACTGTTAACTCACT+AGG - chr1_4:45390965-45390984 Msa0165200:intron 35.0%
! CTTTTGGTATTCATCACTGA+TGG + chr1_4:45391088-45391107 None:intergenic 35.0%
! ATAAAGGACTCGTTGTCTTT+TGG + chr1_4:45391104-45391123 None:intergenic 35.0%
GGAAGATATTGAAGGAACTT+TGG + chr1_4:45391158-45391177 None:intergenic 35.0%
! TCCTTCAATATCTTCCCTTT+TGG - chr1_4:45391162-45391181 Msa0165200:CDS 35.0%
!! TTTTTCAGATGTCGCCAAAA+GGG + chr1_4:45391179-45391198 None:intergenic 35.0%
!! ATTTTTCAGATGTCGCCAAA+AGG + chr1_4:45391180-45391199 None:intergenic 35.0%
TGCTCAAACTCTTGTTCTGA+TGG + chr1_4:45387926-45387945 None:intergenic 40.0%
ATACCAAATTTAGACGCCTC+GGG + chr1_4:45387977-45387996 None:intergenic 40.0%
CATACCAAATTTAGACGCCT+CGG + chr1_4:45387978-45387997 None:intergenic 40.0%
AAACTCGAGTACTTCAGAGA+TGG + chr1_4:45388152-45388171 None:intergenic 40.0%
GACAAAAGAAGCAGGATTAG+AGG + chr1_4:45388423-45388442 None:intergenic 40.0%
TCCGTTAAGACAAAAGAAGC+AGG + chr1_4:45388431-45388450 None:intergenic 40.0%
ACACAATTGTACAGCCTCTT+AGG - chr1_4:45388474-45388493 Msa0165200:CDS 40.0%
CACAATTGTACAGCCTCTTA+GGG - chr1_4:45388475-45388494 Msa0165200:CDS 40.0%
!! CAGATATCGAAAAAGCGTTC+TGG - chr1_4:45388719-45388738 Msa0165200:CDS 40.0%
TTCTGGCACGAAATAGTACA+TGG - chr1_4:45388736-45388755 Msa0165200:CDS 40.0%
GAGAAAGGAACAGTTGAGTA+TGG - chr1_4:45388760-45388779 Msa0165200:CDS 40.0%
TGAGTATGGAGTCAATGTTG+AGG - chr1_4:45388774-45388793 Msa0165200:CDS 40.0%
AGTGATCCTGATGATAAGCT+TGG - chr1_4:45388811-45388830 Msa0165200:CDS 40.0%
CTTGTTCCAAGCTTATCATC+AGG + chr1_4:45388820-45388839 None:intergenic 40.0%
TTCCTGTCAACTAAACGCAA+TGG + chr1_4:45389046-45389065 None:intergenic 40.0%
AGCATCCCAATGTAAAGCAT+AGG + chr1_4:45389173-45389192 None:intergenic 40.0%
AATTGAGAGGATGCAGAACT+AGG + chr1_4:45389373-45389392 None:intergenic 40.0%
!! ACATAATGTGCCGGTTTACA+AGG - chr1_4:45389513-45389532 Msa0165200:CDS 40.0%
GAACACGCGAGATTTGATTA+TGG + chr1_4:45389837-45389856 None:intergenic 40.0%
!! ATTGGTGATTGGTTTCCACT+CGG - chr1_4:45390124-45390143 Msa0165200:intron 40.0%
! TGAATAACGTTTGCTAGCTG+CGG + chr1_4:45390152-45390171 None:intergenic 40.0%
TACAACTCCTCAAAAGCTAG+AGG - chr1_4:45390235-45390254 Msa0165200:intron 40.0%
!!! TTTGTCACCTCTAGCTTTTG+AGG + chr1_4:45390245-45390264 None:intergenic 40.0%
! GATCCAATTTGCCTTTTCCA+TGG - chr1_4:45390469-45390488 Msa0165200:intron 40.0%
AGAATAACGAGGTCTTACCA+TGG + chr1_4:45390489-45390508 None:intergenic 40.0%
! CTCCCGCATAAACAATTTTG+TGG + chr1_4:45390596-45390615 None:intergenic 40.0%
GCCAAAAGGGAAGATATTGA+AGG + chr1_4:45391166-45391185 None:intergenic 40.0%
TACCAAATTTAGACGCCTCG+GGG + chr1_4:45387976-45387995 None:intergenic 45.0%
! GCGTTTTCCAATAGATTGCG+TGG - chr1_4:45388676-45388695 Msa0165200:CDS 45.0%
ACTAAACGCAATGGAGACTG+TGG + chr1_4:45389037-45389056 None:intergenic 45.0%
CTCCATTGCGTTTAGTTGAC+AGG - chr1_4:45389041-45389060 Msa0165200:intron 45.0%
CAAGACATCAGGAGAGAACA+TGG + chr1_4:45389488-45389507 None:intergenic 45.0%
!! TAATGTGCCGGTTTACAAGG+CGG - chr1_4:45389516-45389535 Msa0165200:CDS 45.0%
! TTTTGCACCGCCTTGTAAAC+CGG + chr1_4:45389526-45389545 None:intergenic 45.0%
CTCTCTCTCTCTACATGTGT+TGG - chr1_4:45389641-45389660 Msa0165200:CDS 45.0%
!! TTGGTGATTGGTTTCCACTC+GGG - chr1_4:45390125-45390144 Msa0165200:intron 45.0%
TAAGACATCGCTATCGCAGT+TGG - chr1_4:45390333-45390352 Msa0165200:CDS 45.0%
TGACATGCAGCCTTTGCAAT+AGG - chr1_4:45390404-45390423 Msa0165200:CDS 45.0%
GACATGCAGCCTTTGCAATA+GGG - chr1_4:45390405-45390424 Msa0165200:CDS 45.0%
ACGAGGTCTTACCATGGAAA+AGG + chr1_4:45390483-45390502 None:intergenic 45.0%
TGCTGCCAAAAGTCTTCAAC+AGG - chr1_4:45390661-45390680 Msa0165200:CDS 45.0%
ACCAAATTTAGACGCCTCGG+GGG + chr1_4:45387975-45387994 None:intergenic 50.0%
AAGGTGACGACAAACTCTCC+TGG + chr1_4:45389550-45389569 None:intergenic 50.0%
TGCAGCAGCAGAGGAACAAT+AGG - chr1_4:45389804-45389823 Msa0165200:intron 50.0%
!! TGGTGATTGGTTTCCACTCG+GGG - chr1_4:45390126-45390145 Msa0165200:intron 50.0%
GTGAGCTTGATGTTGTTCGC+AGG + chr1_4:45391016-45391035 None:intergenic 50.0%
TCTGCAGAAAATTGCCCCCG+AGG - chr1_4:45387958-45387977 Msa0165200:CDS 55.0%
GCAGGATTAGAGGCAGCGAT+TGG + chr1_4:45388413-45388432 None:intergenic 55.0%
TACAAGGCGGTGCAAAAGCC+AGG - chr1_4:45389529-45389548 Msa0165200:CDS 55.0%
GTTCCTCTGCTGCTGCATAG+TGG + chr1_4:45389801-45389820 None:intergenic 55.0%
GCAGGAAGTGAAATTCCGCC+AGG + chr1_4:45389963-45389982 None:intergenic 55.0%
! GCCCCCGAGGCGTCTAAATT+TGG - chr1_4:45387971-45387990 Msa0165200:CDS 60.0%
AATGGAGACTGTGGCAGCCG+CGG + chr1_4:45389028-45389047 None:intergenic 60.0%
CCTCTGCTGCTGCATAGTGG+CGG + chr1_4:45389798-45389817 None:intergenic 60.0%
CCGCCACTATGCAGCAGCAG+AGG - chr1_4:45389795-45389814 Msa0165200:intron 65.0%
TTGCTAGCTGCGGCCCCGAG+TGG + chr1_4:45390142-45390161 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 45387887 45391207 45387887 ID=Msa0165200;Name=Msa0165200
chr1_4 mRNA 45387887 45391207 45387887 ID=Msa0165200-mRNA-1;Parent=Msa0165200;Name=Msa0165200-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0|1|1|1|9|0|580
chr1_4 exon 45391102 45391207 45391102 ID=Msa0165200-mRNA-1:exon:12991;Parent=Msa0165200-mRNA-1
chr1_4 exon 45390548 45390704 45390548 ID=Msa0165200-mRNA-1:exon:12990;Parent=Msa0165200-mRNA-1
chr1_4 exon 45390239 45390458 45390239 ID=Msa0165200-mRNA-1:exon:12989;Parent=Msa0165200-mRNA-1
chr1_4 exon 45390022 45390075 45390022 ID=Msa0165200-mRNA-1:exon:12988;Parent=Msa0165200-mRNA-1
chr1_4 exon 45389857 45389936 45389857 ID=Msa0165200-mRNA-1:exon:12987;Parent=Msa0165200-mRNA-1
chr1_4 exon 45389493 45389770 45389493 ID=Msa0165200-mRNA-1:exon:12986;Parent=Msa0165200-mRNA-1
chr1_4 exon 45388604 45389002 45388604 ID=Msa0165200-mRNA-1:exon:12985;Parent=Msa0165200-mRNA-1
chr1_4 exon 45388334 45388509 45388334 ID=Msa0165200-mRNA-1:exon:12984;Parent=Msa0165200-mRNA-1
chr1_4 exon 45387887 45388159 45387887 ID=Msa0165200-mRNA-1:exon:12983;Parent=Msa0165200-mRNA-1
chr1_4 CDS 45391102 45391207 45391102 ID=Msa0165200-mRNA-1:cds;Parent=Msa0165200-mRNA-1
chr1_4 CDS 45390548 45390704 45390548 ID=Msa0165200-mRNA-1:cds;Parent=Msa0165200-mRNA-1
chr1_4 CDS 45390239 45390458 45390239 ID=Msa0165200-mRNA-1:cds;Parent=Msa0165200-mRNA-1
chr1_4 CDS 45390022 45390075 45390022 ID=Msa0165200-mRNA-1:cds;Parent=Msa0165200-mRNA-1
chr1_4 CDS 45389857 45389936 45389857 ID=Msa0165200-mRNA-1:cds;Parent=Msa0165200-mRNA-1
chr1_4 CDS 45389493 45389770 45389493 ID=Msa0165200-mRNA-1:cds;Parent=Msa0165200-mRNA-1
chr1_4 CDS 45388604 45389002 45388604 ID=Msa0165200-mRNA-1:cds;Parent=Msa0165200-mRNA-1
chr1_4 CDS 45388334 45388509 45388334 ID=Msa0165200-mRNA-1:cds;Parent=Msa0165200-mRNA-1
chr1_4 CDS 45387887 45388159 45387887 ID=Msa0165200-mRNA-1:cds;Parent=Msa0165200-mRNA-1
Gene Sequence

>Msa0165200

ATGACGAACAATACTCAAGAATGTCCAACGTATTATCCATCAGAACAAGAGTTTGAGCATCCTTTAGTTTATCTGCAGAAAATTGCCCCCGAGGCGTCTAAATTTGGTATATGCAAAATTGTTTCACCAATCGCTGCCTCTAATCCTGCTTCTTTTGTCTTAACGGAAGAGAAAAGGGATTTTAAGTTTAACACAATTGTACAGCCTCTTAGGGTTTTCGAATCAAATGAGATGGATAAAATAACTTTTTCTAAGAGAAAAAGAAAATATACATATCATGAATTTGAAGCTCTTGCAAACAAGGCGTTTTCCAATAGATTGCGTGGTAAAGAAGATCTCTCTTGTTCAGATATCGAAAAAGCGTTCTGGCACGAAATAGTACATGGAGAGAAAGGAACAGTTGAGTATGGAGTCAATGTTGAGGAAAGCGCCTTTTCAAGTGATCCTGATGATAAGCTTGGAACAAGCAATTTCAATTTGAAGAATTTGCCGCGGCTGCCACAGTCTCCATTGCGTTTAGTTGACAGGAAAATTCCAGGATTAACTGATCCTATGCTTTACATTGGGATGCTATTCAGTATGTTTGCATGGCATGTTGAAGATCACTATTTGTACAGCATAAATTACCATCACTCAGGTGCAAATAAAACTTGGTATGGTGTGCCTAGTTCTGCATCCTCTCAATTTGAAAAGACTGTCTTGAATCATGTGTATTGCAACAAAGTCTTAAAAGAACATGGAGAGAATGGTGCATTCCAATTTCTTGCACAAAAAACAACCATGTTCTCTCCTGATGTCTTGTTAGAACATAATGTGCCGGTTTACAAGGCGGTGCAAAAGCCAGGAGAGTTTGTCGTCACCTTTCCAAATTCATATCATGCTGGATTTAGTCATGGTTTTAACTGTGGAGAAGCTGTAAACTTTGCTATTGGTGATTGGTTTCCACTCGGGGCCGCAGCTAGCAAACGTTATTCACATCTCAAAATGCTACCTATAATTCCATATGAGGAACTTCTTTGTAAAGAAGCATTGCTTATCTACAACTCCTCAAAAGCTAGAGGTGACAAAATCAAACTTGAAGATACGGCAACTTATCGAGCTATTGCACTATCTTTCTTGCATCTTATTCAATTTTATAAGACATCGCTATCGCAGTTGGACAGTTCGAAAAAGTTGTCAAGCTCTTCAAATACATCATTAGGTTCTGTGACATGCAGCCTTTGCAATAGGGATTGTTATGTAGCTTTCTTGTTGTGCAAGAAATGCTATTCAGATCCAATTTGCCTTTTCCATGACAATGTGCCACAAAATTGTTTATGCGGGAGAGAACATACTGTATTTAAGAGGAATGACATGTTGGAACTCGAAGATGCTGCCAAAAGTCTTCAACAGGAAAAAGAATACACCAACGCGTTACCTTTAACAAATATATTCTCCTATAACATAAATGAATGCATCAAAGAACACAAGAATAATAATTGGCATGAGGAAAAAAGTGCTAGAAGAACTGTTAACTCACTAGGTGCAACATCTAAACTGAAAACAAGACCTGCGAACAACATCAAGCTCACTGTTGAAAATAATCAAAAGACAAAGAATCGTTGTAGAAACAATGTGTCTCCATCAGTGATGAATACCAAAAGACAACGAGTCCTTTATAACTTGAGAAAACATGAATCGGAACTGATTACCAAAGTTCCTTCAATATCTTCCCTTTTGGCGACATCTGAAAAATCAAATTAA
Protein sequence

>Msa0165200

MTNNTQECPTYYPSEQEFEHPLVYLQKIAPEASKFGICKIVSPIAASNPASFVLTEEKRDFKFNTIVQPLRVFESNEMDKITFSKRKRKYTYHEFEALANKAFSNRLRGKEDLSCSDIEKAFWHEIVHGEKGTVEYGVNVEESAFSSDPDDKLGTSNFNLKNLPRLPQSPLRLVDRKIPGLTDPMLYIGMLFSMFAWHVEDHYLYSINYHHSGANKTWYGVPSSASSQFEKTVLNHVYCNKVLKEHGENGAFQFLAQKTTMFSPDVLLEHNVPVYKAVQKPGEFVVTFPNSYHAGFSHGFNCGEAVNFAIGDWFPLGAAASKRYSHLKMLPIIPYEELLCKEALLIYNSSKARGDKIKLEDTATYRAIALSFLHLIQFYKTSLSQLDSSKKLSSSSNTSLGSVTCSLCNRDCYVAFLLCKKCYSDPICLFHDNVPQNCLCGREHTVFKRNDMLELEDAAKSLQQEKEYTNALPLTNIFSYNINECIKEHKNNNWHEEKSARRTVNSLGATSKLKTRPANNIKLTVENNQKTKNRCRNNVSPSVMNTKRQRVLYNLRKHESELITKVPSISSLLATSEKSN*