AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0169890


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0169890 MtrunA17_Chr5g0399781 52.830 53 24 1 4 56 13 64 4.10e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCTTGTGGTTTACATTTCA+AGG 0.339580 1_4:-53397915 Msa0169890:CDS
ATTGGTGTCGGCATCTCTAT+TGG 0.346524 1_4:+53396946 None:intergenic
ATTGGTGTCGGCGTCTCTAT+TGG 0.349108 1_4:+53396928 None:intergenic
CCACAAGCGTTACACAATAT+TGG 0.380139 1_4:+53397929 None:intergenic
TTTCAACAGAAACTCAAGTT+TGG 0.389578 1_4:-53397973 Msa0169890:CDS
AATCTTTCAAACCAAACTTC+AGG 0.411413 1_4:-53397800 Msa0169890:intron
CCAAGCAATGAGATAGGTCT+TGG 0.425187 1_4:-53396799 Msa0169890:CDS
CACAATATTGGCGTCTCGTA+TGG 0.440494 1_4:+53397941 None:intergenic
TCAAACAACAAAATATCGTC+AGG 0.502407 1_4:+53396841 Msa0169880:intron
TCTTGGATGCATTCGTCTAA+AGG 0.502885 1_4:-53396782 Msa0169890:CDS
TTTATACCAAGCAATGAGAT+AGG 0.511374 1_4:-53396805 Msa0169890:CDS
CCAATATTGTGTAACGCTTG+TGG 0.532300 1_4:-53397929 Msa0169890:CDS
GAAACTCAAGTTTGGCGCAA+AGG 0.544030 1_4:-53397965 Msa0169890:CDS
CCAAGACCTATCTCATTGCT+TGG 0.545705 1_4:+53396799 Msa0169880:intron
GGTTTACATTTCAAGGCGAA+AGG 0.567422 1_4:-53397908 Msa0169890:CDS
AATAGAGACGCCGACACCAA+TGG 0.575440 1_4:-53396926 Msa0169890:CDS
GTCGGCGTCTCTATTGGTGT+CGG 0.578211 1_4:+53396934 None:intergenic
TCTTCGGGAGATAGTTAGCA+AGG 0.579240 1_4:+53397882 None:intergenic
AAAAGCGTTCATTTGTAGAG+AGG 0.581892 1_4:-53396993 Msa0169890:CDS
GGAAACTCATCCATTGGTGT+CGG 0.599667 1_4:+53396916 None:intergenic
AGCCCTGCAAAATCAGTCAT+CGG 0.602139 1_4:-53397858 Msa0169890:CDS
GATACTTCTACAGATCAAGA+TGG 0.615323 1_4:-53396754 Msa0169890:CDS
CTTCGGGAGATAGTTAGCAA+GGG 0.642252 1_4:+53397883 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAGATGATATAAATTGAA+GGG + chr1_4:53397028-53397047 None:intergenic 15.0%
!! AAATAGATGATATAAATTGA+AGG + chr1_4:53397029-53397048 None:intergenic 15.0%
!!! ACATTGTAAGAATATTTTTA+AGG - chr1_4:53397213-53397232 Msa0169890:intron 15.0%
!!! CATTGTAAGAATATTTTTAA+GGG - chr1_4:53397214-53397233 Msa0169890:intron 15.0%
!!! ATTGTAAGAATATTTTTAAG+GGG - chr1_4:53397215-53397234 Msa0169890:intron 15.0%
!! TTAAAATATGAAACTTCATT+TGG + chr1_4:53397402-53397421 None:intergenic 15.0%
!! AATAGATGATATAAATTGAA+GGG + chr1_4:53397028-53397047 None:intergenic 15.0%
!! AAATAGATGATATAAATTGA+AGG + chr1_4:53397029-53397048 None:intergenic 15.0%
!!! ACATTGTAAGAATATTTTTA+AGG - chr1_4:53397213-53397232 Msa0169890:intron 15.0%
!!! CATTGTAAGAATATTTTTAA+GGG - chr1_4:53397214-53397233 Msa0169890:intron 15.0%
!!! ATTGTAAGAATATTTTTAAG+GGG - chr1_4:53397215-53397234 Msa0169890:intron 15.0%
!! TTAAAATATGAAACTTCATT+TGG + chr1_4:53397402-53397421 None:intergenic 15.0%
!!! AATTTTTATTACCTGAAGTT+TGG + chr1_4:53396941-53396960 None:intergenic 20.0%
!!! AATTTTTATTACCTGAAGTT+TGG + chr1_4:53396941-53396960 None:intergenic 20.0%
!! TTTACATTTGGTGAAATATG+GGG + chr1_4:53396987-53397006 None:intergenic 25.0%
!! CTTTACATTTGGTGAAATAT+GGG + chr1_4:53396988-53397007 None:intergenic 25.0%
!! AATAACTTGTGCTTTACATT+TGG + chr1_4:53396999-53397018 None:intergenic 25.0%
! TCGATTGAAAATGAAAATGT+TGG - chr1_4:53397132-53397151 Msa0169890:intron 25.0%
! CGATTGAAAATGAAAATGTT+GGG - chr1_4:53397133-53397152 Msa0169890:intron 25.0%
!! ATGGGTATTGTTATAATTCA+GGG - chr1_4:53397246-53397265 Msa0169890:intron 25.0%
! ACAAATATCTCCATAAATGT+AGG + chr1_4:53397496-53397515 None:intergenic 25.0%
! GTGGGAAAAATACAAATTTA+TGG - chr1_4:53397692-53397711 Msa0169890:intron 25.0%
!! TTTACATTTGGTGAAATATG+GGG + chr1_4:53396987-53397006 None:intergenic 25.0%
!! CTTTACATTTGGTGAAATAT+GGG + chr1_4:53396988-53397007 None:intergenic 25.0%
!! AATAACTTGTGCTTTACATT+TGG + chr1_4:53396999-53397018 None:intergenic 25.0%
! TCGATTGAAAATGAAAATGT+TGG - chr1_4:53397132-53397151 Msa0169890:intron 25.0%
! CGATTGAAAATGAAAATGTT+GGG - chr1_4:53397133-53397152 Msa0169890:intron 25.0%
!! ATGGGTATTGTTATAATTCA+GGG - chr1_4:53397246-53397265 Msa0169890:intron 25.0%
! ACAAATATCTCCATAAATGT+AGG + chr1_4:53397496-53397515 None:intergenic 25.0%
! GTGGGAAAAATACAAATTTA+TGG - chr1_4:53397692-53397711 Msa0169890:intron 25.0%
TTTCAACAGAAACTCAAGTT+TGG - chr1_4:53396754-53396773 Msa0169890:CDS 30.0%
!!! CAGATGTTTTTTCAATGTCA+AGG + chr1_4:53396896-53396915 None:intergenic 30.0%
AATCTTTCAAACCAAACTTC+AGG - chr1_4:53396927-53396946 Msa0169890:CDS 30.0%
! GCTTTACATTTGGTGAAATA+TGG + chr1_4:53396989-53397008 None:intergenic 30.0%
!! ATTTTTCCTTTTCTAGTGCA+TGG - chr1_4:53397045-53397064 Msa0169890:intron 30.0%
!! TTTTTCCTTTTCTAGTGCAT+GGG - chr1_4:53397046-53397065 Msa0169890:intron 30.0%
ACTCATAAGTAGTGTAGAAT+GGG - chr1_4:53397160-53397179 Msa0169890:intron 30.0%
!! TTTTTAAGGGGTACAATTCA+TGG - chr1_4:53397227-53397246 Msa0169890:intron 30.0%
!! TTTTAAGGGGTACAATTCAT+GGG - chr1_4:53397228-53397247 Msa0169890:intron 30.0%
! CATGGGTATTGTTATAATTC+AGG - chr1_4:53397245-53397264 Msa0169890:intron 30.0%
! TGGGTATTGTTATAATTCAG+GGG - chr1_4:53397247-53397266 Msa0169890:intron 30.0%
!! ATTTTAAGTAGACACAGCAT+TGG - chr1_4:53397415-53397434 Msa0169890:intron 30.0%
ACTCTATATGCCTACATTTA+TGG - chr1_4:53397483-53397502 Msa0169890:intron 30.0%
AATGACAACTAAAGAAGAGT+TGG - chr1_4:53397524-53397543 Msa0169890:intron 30.0%
CTAGCTAATGTGTAACTATA+AGG + chr1_4:53397575-53397594 None:intergenic 30.0%
CACATTAGCTAGTATTCTTA+TGG - chr1_4:53397583-53397602 Msa0169890:intron 30.0%
!!! CATTAGTACCTTATTTTCTC+AGG - chr1_4:53397670-53397689 Msa0169890:intron 30.0%
!!! ACAAATGAACGCTTTTTTTG+AGG + chr1_4:53397730-53397749 None:intergenic 30.0%
! TATAGACTAAGAAGCTTTTG+TGG + chr1_4:53397835-53397854 None:intergenic 30.0%
TCAAACAACAAAATATCGTC+AGG + chr1_4:53397889-53397908 None:intergenic 30.0%
TTTATACCAAGCAATGAGAT+AGG - chr1_4:53397922-53397941 Msa0169890:CDS 30.0%
TTTCAACAGAAACTCAAGTT+TGG - chr1_4:53396754-53396773 Msa0169890:CDS 30.0%
!!! CAGATGTTTTTTCAATGTCA+AGG + chr1_4:53396896-53396915 None:intergenic 30.0%
AATCTTTCAAACCAAACTTC+AGG - chr1_4:53396927-53396946 Msa0169890:CDS 30.0%
! GCTTTACATTTGGTGAAATA+TGG + chr1_4:53396989-53397008 None:intergenic 30.0%
!! ATTTTTCCTTTTCTAGTGCA+TGG - chr1_4:53397045-53397064 Msa0169890:intron 30.0%
!! TTTTTCCTTTTCTAGTGCAT+GGG - chr1_4:53397046-53397065 Msa0169890:intron 30.0%
ACTCATAAGTAGTGTAGAAT+GGG - chr1_4:53397160-53397179 Msa0169890:intron 30.0%
!! TTTTTAAGGGGTACAATTCA+TGG - chr1_4:53397227-53397246 Msa0169890:intron 30.0%
!! TTTTAAGGGGTACAATTCAT+GGG - chr1_4:53397228-53397247 Msa0169890:intron 30.0%
! CATGGGTATTGTTATAATTC+AGG - chr1_4:53397245-53397264 Msa0169890:intron 30.0%
! TGGGTATTGTTATAATTCAG+GGG - chr1_4:53397247-53397266 Msa0169890:intron 30.0%
!! ATTTTAAGTAGACACAGCAT+TGG - chr1_4:53397415-53397434 Msa0169890:intron 30.0%
ACTCTATATGCCTACATTTA+TGG - chr1_4:53397483-53397502 Msa0169890:intron 30.0%
AATGACAACTAAAGAAGAGT+TGG - chr1_4:53397524-53397543 Msa0169890:intron 30.0%
CTAGCTAATGTGTAACTATA+AGG + chr1_4:53397575-53397594 None:intergenic 30.0%
CACATTAGCTAGTATTCTTA+TGG - chr1_4:53397583-53397602 Msa0169890:intron 30.0%
!!! CATTAGTACCTTATTTTCTC+AGG - chr1_4:53397670-53397689 Msa0169890:intron 30.0%
!!! ACAAATGAACGCTTTTTTTG+AGG + chr1_4:53397730-53397749 None:intergenic 30.0%
! TATAGACTAAGAAGCTTTTG+TGG + chr1_4:53397835-53397854 None:intergenic 30.0%
TCAAACAACAAAATATCGTC+AGG + chr1_4:53397889-53397908 None:intergenic 30.0%
TTTATACCAAGCAATGAGAT+AGG - chr1_4:53397922-53397941 Msa0169890:CDS 30.0%
TAAAACCCATGCACTAGAAA+AGG + chr1_4:53397054-53397073 None:intergenic 35.0%
GACTCATAAGTAGTGTAGAA+TGG - chr1_4:53397159-53397178 Msa0169890:intron 35.0%
! TCGTTGCAATGTGGATTAAA+GGG - chr1_4:53397365-53397384 Msa0169890:intron 35.0%
!!! TAGTACCTTATTTTCTCAGG+TGG - chr1_4:53397673-53397692 Msa0169890:intron 35.0%
!!! AGTACCTTATTTTCTCAGGT+GGG - chr1_4:53397674-53397693 Msa0169890:intron 35.0%
! TTTTCCCACCTGAGAAAATA+AGG + chr1_4:53397681-53397700 None:intergenic 35.0%
!! AAAAGCGTTCATTTGTAGAG+AGG - chr1_4:53397734-53397753 Msa0169890:intron 35.0%
! TTTTGTGGAAACTCATCCAT+TGG + chr1_4:53397820-53397839 None:intergenic 35.0%
GATACTTCTACAGATCAAGA+TGG - chr1_4:53397973-53397992 Msa0169890:CDS 35.0%
TAAAACCCATGCACTAGAAA+AGG + chr1_4:53397054-53397073 None:intergenic 35.0%
GACTCATAAGTAGTGTAGAA+TGG - chr1_4:53397159-53397178 Msa0169890:intron 35.0%
! TCGTTGCAATGTGGATTAAA+GGG - chr1_4:53397365-53397384 Msa0169890:intron 35.0%
!!! TAGTACCTTATTTTCTCAGG+TGG - chr1_4:53397673-53397692 Msa0169890:intron 35.0%
!!! AGTACCTTATTTTCTCAGGT+GGG - chr1_4:53397674-53397693 Msa0169890:intron 35.0%
! TTTTCCCACCTGAGAAAATA+AGG + chr1_4:53397681-53397700 None:intergenic 35.0%
!! AAAAGCGTTCATTTGTAGAG+AGG - chr1_4:53397734-53397753 Msa0169890:intron 35.0%
! TTTTGTGGAAACTCATCCAT+TGG + chr1_4:53397820-53397839 None:intergenic 35.0%
GATACTTCTACAGATCAAGA+TGG - chr1_4:53397973-53397992 Msa0169890:CDS 35.0%
CCACAAGCGTTACACAATAT+TGG + chr1_4:53396801-53396820 Msa0169880:intron 40.0%
CCAATATTGTGTAACGCTTG+TGG - chr1_4:53396798-53396817 Msa0169890:CDS 40.0%
CGCTTGTGGTTTACATTTCA+AGG - chr1_4:53396812-53396831 Msa0169890:CDS 40.0%
GGTTTACATTTCAAGGCGAA+AGG - chr1_4:53396819-53396838 Msa0169890:CDS 40.0%
!! GTGCATGGGTTTTAATGTAG+CGG - chr1_4:53397060-53397079 Msa0169890:intron 40.0%
ATAAGTAGTGTAGAATGGGC+AGG - chr1_4:53397164-53397183 Msa0169890:intron 40.0%
! ACGCTGCTAAAGTTTTCTAC+GGG + chr1_4:53397292-53397311 None:intergenic 40.0%
! CACGCTGCTAAAGTTTTCTA+CGG + chr1_4:53397293-53397312 None:intergenic 40.0%
! GTCGTTGCAATGTGGATTAA+AGG - chr1_4:53397364-53397383 Msa0169890:intron 40.0%
!! TGTGGATTAAAGGGTGTTTG+AGG - chr1_4:53397374-53397393 Msa0169890:intron 40.0%
!! GTGGATTAAAGGGTGTTTGA+GGG - chr1_4:53397375-53397394 Msa0169890:intron 40.0%
TCTTGGATGCATTCGTCTAA+AGG - chr1_4:53397945-53397964 Msa0169890:CDS 40.0%
CCACAAGCGTTACACAATAT+TGG + chr1_4:53396801-53396820 Msa0169880:intron 40.0%
CCAATATTGTGTAACGCTTG+TGG - chr1_4:53396798-53396817 Msa0169890:CDS 40.0%
CGCTTGTGGTTTACATTTCA+AGG - chr1_4:53396812-53396831 Msa0169890:CDS 40.0%
GGTTTACATTTCAAGGCGAA+AGG - chr1_4:53396819-53396838 Msa0169890:CDS 40.0%
!! GTGCATGGGTTTTAATGTAG+CGG - chr1_4:53397060-53397079 Msa0169890:intron 40.0%
ATAAGTAGTGTAGAATGGGC+AGG - chr1_4:53397164-53397183 Msa0169890:intron 40.0%
! ACGCTGCTAAAGTTTTCTAC+GGG + chr1_4:53397292-53397311 None:intergenic 40.0%
! CACGCTGCTAAAGTTTTCTA+CGG + chr1_4:53397293-53397312 None:intergenic 40.0%
! GTCGTTGCAATGTGGATTAA+AGG - chr1_4:53397364-53397383 Msa0169890:intron 40.0%
!! TGTGGATTAAAGGGTGTTTG+AGG - chr1_4:53397374-53397393 Msa0169890:intron 40.0%
!! GTGGATTAAAGGGTGTTTGA+GGG - chr1_4:53397375-53397394 Msa0169890:intron 40.0%
TCTTGGATGCATTCGTCTAA+AGG - chr1_4:53397945-53397964 Msa0169890:CDS 40.0%
GAAACTCAAGTTTGGCGCAA+AGG - chr1_4:53396762-53396781 Msa0169890:CDS 45.0%
CACAATATTGGCGTCTCGTA+TGG + chr1_4:53396789-53396808 Msa0169880:intron 45.0%
CTTCGGGAGATAGTTAGCAA+GGG + chr1_4:53396847-53396866 Msa0169880:intron 45.0%
TCTTCGGGAGATAGTTAGCA+AGG + chr1_4:53396848-53396867 Msa0169880:intron 45.0%
! TGATTTTGCAGGGCTCTCTT+CGG + chr1_4:53396864-53396883 Msa0169880:intron 45.0%
AGCCCTGCAAAATCAGTCAT+CGG - chr1_4:53396869-53396888 Msa0169890:CDS 45.0%
!! GTCCGATGACTGATTTTGCA+GGG + chr1_4:53396874-53396893 Msa0169880:intron 45.0%
! CGCTGCTAAAGTTTTCTACG+GGG + chr1_4:53397291-53397310 None:intergenic 45.0%
TGAGAGTTGTCGTTGCAATG+TGG - chr1_4:53397356-53397375 Msa0169890:intron 45.0%
!! ATTGGTGTCGGCATCTCTAT+TGG + chr1_4:53397784-53397803 None:intergenic 45.0%
GGAAACTCATCCATTGGTGT+CGG + chr1_4:53397814-53397833 None:intergenic 45.0%
CCAAGACCTATCTCATTGCT+TGG + chr1_4:53397931-53397950 None:intergenic 45.0%
CCAAGCAATGAGATAGGTCT+TGG - chr1_4:53397928-53397947 Msa0169890:CDS 45.0%
GAAACTCAAGTTTGGCGCAA+AGG - chr1_4:53396762-53396781 Msa0169890:CDS 45.0%
CACAATATTGGCGTCTCGTA+TGG + chr1_4:53396789-53396808 Msa0169880:intron 45.0%
CTTCGGGAGATAGTTAGCAA+GGG + chr1_4:53396847-53396866 Msa0169880:intron 45.0%
TCTTCGGGAGATAGTTAGCA+AGG + chr1_4:53396848-53396867 Msa0169880:intron 45.0%
! TGATTTTGCAGGGCTCTCTT+CGG + chr1_4:53396864-53396883 Msa0169880:intron 45.0%
AGCCCTGCAAAATCAGTCAT+CGG - chr1_4:53396869-53396888 Msa0169890:CDS 45.0%
!! GTCCGATGACTGATTTTGCA+GGG + chr1_4:53396874-53396893 Msa0169880:intron 45.0%
! CGCTGCTAAAGTTTTCTACG+GGG + chr1_4:53397291-53397310 None:intergenic 45.0%
TGAGAGTTGTCGTTGCAATG+TGG - chr1_4:53397356-53397375 Msa0169890:intron 45.0%
!! ATTGGTGTCGGCATCTCTAT+TGG + chr1_4:53397784-53397803 None:intergenic 45.0%
GGAAACTCATCCATTGGTGT+CGG + chr1_4:53397814-53397833 None:intergenic 45.0%
CCAAGACCTATCTCATTGCT+TGG + chr1_4:53397931-53397950 None:intergenic 45.0%
CCAAGCAATGAGATAGGTCT+TGG - chr1_4:53397928-53397947 Msa0169890:CDS 45.0%
! GATTTTGCAGGGCTCTCTTC+GGG + chr1_4:53396863-53396882 Msa0169880:intron 50.0%
!! GGTCCGATGACTGATTTTGC+AGG + chr1_4:53396875-53396894 Msa0169880:intron 50.0%
!! ATTGGTGTCGGCGTCTCTAT+TGG + chr1_4:53397802-53397821 None:intergenic 50.0%
AATAGAGACGCCGACACCAA+TGG - chr1_4:53397801-53397820 Msa0169890:CDS 50.0%
! GATTTTGCAGGGCTCTCTTC+GGG + chr1_4:53396863-53396882 Msa0169880:intron 50.0%
!! GGTCCGATGACTGATTTTGC+AGG + chr1_4:53396875-53396894 Msa0169880:intron 50.0%
!! ATTGGTGTCGGCGTCTCTAT+TGG + chr1_4:53397802-53397821 None:intergenic 50.0%
AATAGAGACGCCGACACCAA+TGG - chr1_4:53397801-53397820 Msa0169890:CDS 50.0%
GTCGGCGTCTCTATTGGTGT+CGG + chr1_4:53397796-53397815 None:intergenic 55.0%
GTCGGCGTCTCTATTGGTGT+CGG + chr1_4:53397796-53397815 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 53396750 53397999 53396750 ID=Msa0169890;Name=Msa0169890
chr1_4 mRNA 53396750 53397999 53396750 ID=Msa0169890-mRNA-1;Parent=Msa0169890;Name=Msa0169890-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|0|0|1|1|1|2|0|168
chr1_4 exon 53397801 53397999 53397801 ID=Msa0169890-mRNA-1:exon:15900;Parent=Msa0169890-mRNA-1
chr1_4 exon 53396750 53397057 53396750 ID=Msa0169890-mRNA-1:exon:15899;Parent=Msa0169890-mRNA-1
chr1_4 CDS 53397801 53397999 53397801 ID=Msa0169890-mRNA-1:cds;Parent=Msa0169890-mRNA-1
chr1_4 CDS 53396750 53397057 53396750 ID=Msa0169890-mRNA-1:cds;Parent=Msa0169890-mRNA-1
Gene Sequence

>Msa0169890

ATGGTTTCAACAGAAACTCAAGTTTGGCGCAAAGGACCATACGAGACGCCAATATTGTGTAACGCTTGTGGTTTACATTTCAAGGCGAAAGGAACCCTTGCTAACTATCTCCCGAAGAGAGCCCTGCAAAATCAGTCATCGGACCTTGACATTGAAAAAACATCTGAAAGTAATGCAAATCTTTCAAACCAAACTTCAGGTGGGAAAAATACAAATTTATGGAACGTCAAAATTCCTCAAAAAAAGCGTTCATTTGTAGAGAGGCATTCATCGACTGTGATGCAGACACCAATAGAGATGCCGACACCAATAGAGACGCCGACACCAATGGATGAGTTTCCACAAAAGCTTCTTAGTCTATATGAATCTGAGAAAGAAGAAAATGAATCATCTCCTGACGATATTTTGTTGTTTGAGAAAATGAACAACTTTATACCAAGCAATGAGATAGGTCTTGGATGCATTCGTCTAAAGGCAGATGATACTTCTACAGATCAAGATGGTTAA

Protein sequence

>Msa0169890

MVSTETQVWRKGPYETPILCNACGLHFKAKGTLANYLPKRALQNQSSDLDIEKTSESNANLSNQTSGGKNTNLWNVKIPQKKRSFVERHSSTVMQTPIEMPTPIETPTPMDEFPQKLLSLYESEKEENESSPDDILLFEKMNNFIPSNEIGLGCIRLKADDTSTDQDG*