AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0170400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0170400 MtrunA17_Chr7g0252101 50.520 481 181 9 76 549 2 432 2.22e-135 400
Msa0170400 MtrunA17_Chr4g0075971 46.691 559 243 10 5 549 85 602 8.04e-134 402
Msa0170400 MtrunA17_Chr4g0075971 44.444 153 79 2 8 157 11 160 3.56e-27 115
Msa0170400 MtrunA17_Chr1g0207351 60.458 349 108 6 208 549 41 366 4.82e-125 371
Msa0170400 MtrunA17_Chr3g0093911 58.501 347 113 4 208 549 30 350 2.71e-122 363
Msa0170400 MtrunA17_Chr1g0209051 61.029 272 96 6 285 548 1 270 1.08e-98 300
Msa0170400 MtrunA17_Chr3g0077801 48.711 349 140 8 208 546 30 349 1.24e-86 272
Msa0170400 MtrunA17_Chr6g0488101 39.959 488 225 18 88 549 10 455 2.96e-85 271
Msa0170400 MtrunA17_Chr6g0488271 40.889 450 205 16 119 549 8 415 6.10e-82 261
Msa0170400 MtrunA17_Chr3g0077901 46.154 351 150 8 208 548 50 371 3.86e-80 256
Msa0170400 MtrunA17_Chr1g0187941 90.511 137 10 1 376 509 1 137 9.59e-80 246
Msa0170400 MtrunA17_Chr7g0243331 47.458 354 141 11 208 549 30 350 2.22e-77 248
Msa0170400 MtrunA17_Chr3g0077851 44.729 351 155 8 208 548 14 335 3.81e-76 244
Msa0170400 MtrunA17_Chr3g0112311 43.575 358 152 10 208 547 30 355 1.00e-69 228
Msa0170400 MtrunA17_Chr6g0452641 45.000 320 150 10 249 549 28 340 9.10e-62 206
Msa0170400 MtrunA17_Chr4g0075861 41.463 328 147 5 4 324 84 373 1.96e-60 204
Msa0170400 MtrunA17_Chr4g0075861 44.444 153 79 2 8 157 11 160 9.60e-29 117
Msa0170400 MtrunA17_Chr1g0202841 62.011 179 63 2 376 549 1 179 2.59e-60 197
Msa0170400 MtrunA17_Chr3g0122991 40.557 323 101 10 208 515 28 274 1.73e-53 182
Msa0170400 MtrunA17_Chr3g0123471 45.736 258 125 7 293 541 8 259 7.46e-53 180
Msa0170400 MtrunA17_Chr1g0206771 47.260 146 75 1 11 154 18 163 9.65e-37 134
Msa0170400 MtrunA17_Chr1g0206771 52.113 71 34 0 82 152 12 82 1.23e-16 78.2
Msa0170400 MtrunA17_Chr3g0077861 46.584 161 74 3 9 160 8 165 3.01e-33 124
Msa0170400 MtrunA17_Chr3g0077911 46.795 156 71 3 9 155 8 160 5.23e-33 123
Msa0170400 MtrunA17_Chr3g0077911 50.000 72 33 1 86 157 8 76 3.79e-13 67.4
Msa0170400 MtrunA17_Chr1g0205821 41.401 157 83 1 8 155 12 168 9.90e-33 123
Msa0170400 MtrunA17_Chr1g0205821 40.476 84 50 0 4 87 94 177 4.56e-15 73.6
Msa0170400 MtrunA17_Chr3g0077891 43.713 167 79 3 9 160 8 174 2.19e-31 119
Msa0170400 MtrunA17_Chr3g0077941 43.373 166 80 3 9 160 8 173 4.33e-31 119
Msa0170400 MtrunA17_Chr7g0243391 44.231 208 82 6 208 410 47 225 1.08e-30 120
Msa0170400 MtrunA17_Chr3g0077881 43.114 167 80 3 9 160 8 174 1.30e-29 114
Msa0170400 MtrunA17_Chr1g0205831 39.456 147 83 1 11 157 4 144 2.75e-29 114
Msa0170400 MtrunA17_Chr5g0415031 43.038 158 79 2 1 149 37 192 3.23e-29 123
Msa0170400 MtrunA17_Chr5g0415031 57.143 77 33 0 85 161 42 118 6.16e-19 91.3
Msa0170400 MtrunA17_Chr1g0209201 44.056 143 72 2 1 136 1 142 5.58e-28 109
Msa0170400 MtrunA17_Chr1g0209201 46.835 79 41 1 89 167 19 96 6.78e-15 72.0
Msa0170400 MtrunA17_Chr8g0364231 42.038 157 80 2 1 148 36 190 2.27e-27 117
Msa0170400 MtrunA17_Chr8g0364231 51.685 89 37 1 85 173 41 123 1.54e-19 93.2
Msa0170400 MtrunA17_Chr1g0209191 58.416 101 32 2 455 548 1 98 3.57e-27 105
Msa0170400 MtrunA17_Chr1g0196241 60.440 91 32 2 444 533 1 88 1.09e-26 103
Msa0170400 MtrunA17_Chr6g0486591 43.210 162 74 5 403 549 1 159 3.17e-26 104
Msa0170400 MtrunA17_Chr6g0452021 42.177 147 78 2 9 148 43 189 3.29e-26 112
Msa0170400 MtrunA17_Chr6g0452021 60.000 70 28 0 86 155 43 112 1.85e-17 85.1
Msa0170400 MtrunA17_Chr1g0206951 53.097 113 25 3 208 318 28 114 4.93e-26 103
Msa0170400 MtrunA17_Chr6g0452001 41.401 157 81 2 1 148 57 211 4.97e-26 113
Msa0170400 MtrunA17_Chr6g0452001 61.972 71 27 0 85 155 62 132 1.03e-18 90.5
Msa0170400 MtrunA17_Chr1g0206781 49.550 111 39 2 11 121 13 106 5.69e-23 94.4
Msa0170400 MtrunA17_Chr1g0206781 62.687 67 25 0 88 154 13 79 2.80e-20 86.7
Msa0170400 MtrunA17_Chr7g0218891 39.583 144 78 3 27 161 119 262 2.76e-20 90.9
Msa0170400 MtrunA17_Chr7g0218891 36.646 161 91 5 12 161 16 176 3.43e-16 79.0
Msa0170400 MtrunA17_Chr2g0328701 45.545 101 49 2 8 105 28 125 6.32e-17 77.4
Msa0170400 MtrunA17_Chr2g0328701 48.611 72 34 1 85 156 28 96 3.87e-13 66.6
Msa0170400 MtrunA17_Chr6g0459061 29.381 194 118 9 362 545 170 354 2.03e-16 81.3
Msa0170400 MtrunA17_Chr1g0205811 41.379 87 51 0 86 172 14 100 1.53e-15 73.2
Msa0170400 MtrunA17_Chr1g0205811 42.308 78 45 0 9 86 14 91 3.41e-14 69.3
Msa0170400 MtrunA17_Chr7g0218911 45.455 88 44 1 13 96 2 89 6.34e-15 71.2
Msa0170400 MtrunA17_Chr7g0218911 50.746 67 33 0 90 156 2 68 9.15e-14 68.2
Msa0170400 MtrunA17_Chr1g0205861 41.429 70 41 0 8 77 15 84 2.12e-13 67.0
Msa0170400 MtrunA17_Chr1g0205861 42.857 70 40 0 85 154 15 84 2.78e-13 66.6
Msa0170400 MtrunA17_Chr8g0364021 44.156 77 43 0 84 160 42 118 2.25e-13 70.1
Msa0170400 MtrunA17_Chr8g0364021 44.444 72 40 0 7 78 42 113 9.11e-13 68.2
Msa0170400 MtrunA17_Chr1g0205801 39.286 84 49 1 9 90 12 95 5.73e-12 62.8
Msa0170400 MtrunA17_Chr1g0205801 35.354 99 52 1 86 172 12 110 7.90e-12 62.4
Msa0170400 MtrunA17_Chr1g0156171 61.364 44 17 0 116 159 17 60 5.45e-11 60.1
Msa0170400 MtrunA17_Chr1g0156171 67.500 40 13 0 39 78 17 56 6.18e-11 60.1
Msa0170400 MtrunA17_Chr3g0077811 51.852 54 26 0 27 80 32 85 7.76e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0170400 AT5G15020.2 44.516 155 77 3 1 148 46 198 1.19e-28 122
Msa0170400 AT5G15020.2 61.972 71 27 0 85 155 51 121 6.59e-18 88.2
Msa0170400 AT5G15020.1 44.516 155 77 3 1 148 46 198 1.25e-28 122
Msa0170400 AT5G15020.1 61.972 71 27 0 85 155 51 121 6.84e-18 88.2
Msa0170400 AT3G01320.2 39.378 193 85 4 1 182 51 222 2.25e-28 120
Msa0170400 AT3G01320.1 39.378 193 85 4 1 182 51 222 2.37e-28 120
Msa0170400 AT1G24190.2 41.401 157 81 2 1 148 8 162 5.34e-26 113
Msa0170400 AT1G24190.2 61.972 71 27 0 85 155 13 83 6.40e-19 91.7
Msa0170400 AT1G24190.1 41.401 157 81 2 1 148 8 162 5.59e-26 113
Msa0170400 AT1G24190.1 61.972 71 27 0 85 155 13 83 6.52e-19 91.7
Msa0170400 AT1G24190.3 41.401 157 81 2 1 148 8 162 6.35e-26 113
Msa0170400 AT1G24190.3 61.972 71 27 0 85 155 13 83 6.48e-19 91.7
Msa0170400 AT1G70060.2 41.772 158 80 3 1 148 8 163 1.05e-24 109
Msa0170400 AT1G70060.2 54.217 83 38 0 86 168 14 96 1.00e-17 87.8
Msa0170400 AT1G70060.1 41.772 158 80 3 1 148 8 163 1.05e-24 109
Msa0170400 AT1G70060.1 54.217 83 38 0 86 168 14 96 1.00e-17 87.8
Msa0170400 AT1G24230.1 35.714 154 92 3 8 160 13 160 2.01e-23 99.8
Msa0170400 AT1G24230.1 30.137 146 95 4 5 144 83 227 1.36e-12 68.2
Msa0170400 AT1G10450.3 39.456 147 82 1 9 148 84 230 2.91e-23 105
Msa0170400 AT1G10450.3 51.351 74 36 0 82 155 80 153 7.33e-14 75.5
Msa0170400 AT1G10450.2 39.456 147 82 1 9 148 33 179 3.28e-23 105
Msa0170400 AT1G10450.2 51.351 74 36 0 82 155 29 102 7.87e-14 75.1
Msa0170400 AT1G10450.1 39.456 147 82 1 9 148 33 179 3.28e-23 105
Msa0170400 AT1G10450.1 51.351 74 36 0 82 155 29 102 7.87e-14 75.1
Msa0170400 AT1G59890.4 40.816 147 80 2 9 148 45 191 9.89e-23 103
Msa0170400 AT1G59890.4 57.143 70 30 0 86 155 45 114 1.81e-16 83.6
Msa0170400 AT1G59890.2 40.816 147 80 2 9 148 45 191 1.14e-22 103
Msa0170400 AT1G59890.2 57.143 70 30 0 86 155 45 114 1.93e-16 83.6
Msa0170400 AT1G59890.3 40.816 147 80 2 9 148 45 191 1.17e-22 103
Msa0170400 AT1G59890.3 57.143 70 30 0 86 155 45 114 1.96e-16 83.6
Msa0170400 AT1G59890.1 40.816 147 80 2 9 148 45 191 1.21e-22 103
Msa0170400 AT1G59890.1 57.143 70 30 0 86 155 45 114 1.93e-16 83.6
Msa0170400 AT1G24200.1 35.948 153 90 2 2 148 9 159 5.03e-21 91.7
Msa0170400 AT1G24200.1 42.268 97 42 1 81 177 9 91 3.60e-16 77.8
Msa0170400 AT1G70030.1 37.419 155 86 3 2 148 6 157 3.07e-20 88.2
Msa0170400 AT1G70030.1 48.571 70 36 0 86 155 11 80 1.04e-12 66.6
Msa0170400 AT1G27260.1 31.579 152 91 1 12 150 6 157 1.95e-17 82.0
Msa0170400 AT1G24250.1 28.902 173 94 2 4 147 9 181 3.66e-16 79.0
Msa0170400 AT1G23810.1 29.630 162 96 2 4 147 9 170 1.35e-15 77.0
Msa0170400 AT5G15025.1 50.704 71 35 0 8 78 46 116 2.46e-15 73.2
Msa0170400 AT5G15025.1 50.704 71 35 0 85 155 46 116 3.70e-15 72.4
Msa0170400 AT1G27280.1 28.272 191 125 3 27 206 11 200 4.78e-15 75.1
Msa0170400 AT1G70030.2 38.776 147 63 6 2 148 17 136 7.63e-15 72.0
Msa0170400 AT1G70030.2 48.571 70 36 0 86 155 22 91 7.62e-13 66.6
Msa0170400 AT1G27220.1 46.753 77 41 0 85 161 13 89 5.21e-14 70.9
Msa0170400 AT1G27220.1 40.206 97 56 1 8 104 13 107 2.39e-12 66.2
Msa0170400 AT1G27250.1 39.583 96 57 1 2 96 14 109 5.76e-14 69.7
Msa0170400 AT1G27250.1 47.826 69 36 0 85 153 20 88 1.03e-13 68.9
Msa0170400 AT4G12020.11 43.636 110 52 4 86 191 308 411 6.53e-14 75.5
Msa0170400 AT4G12020.11 42.718 103 53 3 9 111 308 404 1.36e-13 74.7
Msa0170400 AT4G12020.6 43.636 110 52 4 86 191 308 411 8.80e-14 75.1
Msa0170400 AT4G12020.6 42.718 103 53 3 9 111 308 404 1.67e-13 74.3
Msa0170400 AT4G12020.8 43.636 110 52 4 86 191 308 411 8.80e-14 75.1
Msa0170400 AT4G12020.8 42.718 103 53 3 9 111 308 404 1.67e-13 74.3
Msa0170400 AT4G12020.7 43.636 110 52 4 86 191 308 411 9.62e-14 75.1
Msa0170400 AT4G12020.7 42.718 103 53 3 9 111 308 404 1.73e-13 74.3
Msa0170400 AT4G12020.1 43.636 110 52 4 86 191 308 411 9.71e-14 75.1
Msa0170400 AT4G12020.1 42.718 103 53 3 9 111 308 404 1.87e-13 74.3
Msa0170400 AT4G12020.9 43.636 110 52 4 86 191 308 411 9.71e-14 75.1
Msa0170400 AT4G12020.9 42.718 103 53 3 9 111 308 404 1.87e-13 74.3
Msa0170400 AT4G12020.3 43.636 110 52 4 86 191 308 411 9.79e-14 75.1
Msa0170400 AT4G12020.3 42.718 103 53 3 9 111 308 404 1.67e-13 74.3
Msa0170400 AT4G12020.10 43.636 110 52 4 86 191 308 411 9.87e-14 75.1
Msa0170400 AT4G12020.10 42.718 103 53 3 9 111 308 404 1.70e-13 74.3
Msa0170400 AT4G12020.2 43.636 110 52 4 86 191 308 411 1.02e-13 75.1
Msa0170400 AT4G12020.2 42.718 103 53 3 9 111 308 404 1.76e-13 74.3
Msa0170400 AT4G12020.5 43.636 110 52 4 86 191 241 344 1.02e-13 75.1
Msa0170400 AT4G12020.5 42.718 103 53 3 9 111 241 337 1.92e-13 74.3
Msa0170400 AT4G12020.4 43.636 110 52 4 86 191 308 411 1.03e-13 75.1
Msa0170400 AT4G12020.4 42.718 103 53 3 9 111 308 404 1.72e-13 74.3
Msa0170400 AT1G70030.3 45.455 88 41 2 2 89 17 97 1.86e-13 67.8
Msa0170400 AT1G70030.3 48.571 70 36 0 86 155 22 91 2.67e-13 67.4
Msa0170400 AT5G15040.1 44.444 72 40 0 89 160 6 77 6.15e-12 62.4
Msa0170400 AT5G15040.1 45.455 66 36 0 13 78 7 72 2.76e-11 60.5
Msa0170400 AT5G15040.3 44.444 72 40 0 89 160 6 77 6.15e-12 62.4
Msa0170400 AT5G15040.3 45.455 66 36 0 13 78 7 72 2.76e-11 60.5
Msa0170400 AT5G15040.2 44.444 72 40 0 89 160 6 77 6.15e-12 62.4
Msa0170400 AT5G15040.2 45.455 66 36 0 13 78 7 72 2.76e-11 60.5

Find 109 sgRNAs with CRISPR-Local

Find 296 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTTCTTTGTTCTTGTAAA+TGG 0.060967 1_4:+54384193 None:intergenic
AGGGCATACATATTTAATTT+TGG 0.100116 1_4:-54387095 Msa0170400:CDS
AATATATGTTGTCACTTTAA+AGG 0.159016 1_4:-54383966 Msa0170400:CDS
AGGGCATACAAATTTAATTT+TGG 0.163350 1_4:-54387326 Msa0170400:CDS
TATTTGATAGTATCTCAATT+AGG 0.214252 1_4:-54385878 Msa0170400:CDS
AATTTACTGGAGAAATTTCT+TGG 0.242262 1_4:-54384479 Msa0170400:CDS
CCAAGGTTCTTCCTCTGTTC+AGG 0.256642 1_4:-54386051 Msa0170400:intron
TTTATGATGCCGATAATTTC+AGG 0.267835 1_4:-54383733 Msa0170400:CDS
TATCCAACAATGGAAGATAA+AGG 0.272681 1_4:-54384022 Msa0170400:CDS
ATTTGATAGTATCTCAATTA+GGG 0.295295 1_4:-54385877 Msa0170400:CDS
TGACAACATATATTATATTA+TGG 0.302535 1_4:+54383975 None:intergenic
TCCCCTTTATCTTCCATTGT+TGG 0.306610 1_4:+54384019 None:intergenic
TTTCAATTTAACATAATTCA+TGG 0.315727 1_4:+54383778 None:intergenic
CCTTCTTTGTTCTTGTAAAT+GGG 0.316829 1_4:+54384194 None:intergenic
ACAATTTGCCCTGAAATTAT+CGG 0.335045 1_4:+54383724 None:intergenic
ACGATCTCTTTCAGATTTGC+CGG 0.336166 1_4:-54384532 Msa0170400:intron
GTTATAGTAATAAATTTCAT+TGG 0.336574 1_4:+54383880 None:intergenic
ATCCAACAATGGAAGATAAA+GGG 0.341601 1_4:-54384021 Msa0170400:CDS
ATTACGGTTTCAACTCTTGA+TGG 0.343245 1_4:-54383584 Msa0170400:CDS
CAAGATGTAGTGTGTATTCA+TGG 0.347006 1_4:-54383631 Msa0170400:CDS
TTGGTTGGATATGCTAATAA+AGG 0.350245 1_4:+54384327 None:intergenic
GCAGTAAAGGAGCTGTTTAA+AGG 0.354318 1_4:-54387115 Msa0170400:CDS
CAAGTTCTCGCATGTTTGAT+TGG 0.364748 1_4:-54383559 Msa0170400:CDS
AGGAAGAACCTTGGACGGTT+TGG 0.368344 1_4:+54386060 None:intergenic
GCCAGTCGATCATCATGGTT+TGG 0.374032 1_4:+54387253 None:intergenic
TATGTTAAATTGAAAACTTT+GGG 0.374358 1_4:-54383770 Msa0170400:CDS
TTATGTTAAATTGAAAACTT+TGG 0.379240 1_4:-54383771 Msa0170400:CDS
AAGAATGTGTTCAAGATAGA+AGG 0.385935 1_4:-54383806 Msa0170400:CDS
TTGCCGGTGTATGCAAGAAT+TGG 0.388370 1_4:-54384516 Msa0170400:CDS
AGGGGAAGTGGTTGACTTTG+TGG 0.392069 1_4:-54384002 Msa0170400:CDS
GCGGTAAAGGAGCTGTTTAA+AGG 0.393317 1_4:-54387346 Msa0170400:CDS
TATATGTGATGAGCAACTTT+TGG 0.394099 1_4:-54383846 Msa0170400:CDS
CCAGTCGATCATCATGGTTT+GGG 0.394292 1_4:+54387254 None:intergenic
CCATTCATCTCAAGTTGAAA+TGG 0.398941 1_4:+54387031 None:intergenic
AAACGTTCTGATGAGTTTCA+AGG 0.401132 1_4:-54387170 Msa0170400:CDS
CATGTCTATCAATCTCAAAA+TGG 0.409694 1_4:-54384058 Msa0170400:CDS
AGTTTATTGCTTGAAGATGA+TGG 0.413789 1_4:-54384302 Msa0170400:CDS
ATCAATCTCAAAATGGAGAA+TGG 0.414871 1_4:-54384051 Msa0170400:CDS
ACAATTATAATCGTTAATTC+CGG 0.416186 1_4:+54384156 None:intergenic
CTCTCATATCCCCACAAGTT+CGG 0.416424 1_4:+54383686 None:intergenic
CCATTTCAACTTGAGATGAA+TGG 0.420898 1_4:-54387031 Msa0170400:intron
TTTCAGTTAGGGGTGAGCTT+TGG 0.426113 1_4:-54385905 Msa0170400:intron
GAAGAACCATTACTACTTCA+AGG 0.427911 1_4:-54384439 Msa0170400:CDS
TTCATGTTCCTTTGGTAGGA+AGG 0.439686 1_4:+54387063 None:intergenic
AGGTGTCGTGGCAGCAGTAA+AGG 0.441612 1_4:-54387128 Msa0170400:CDS
AGGTGTCGTGGCAGCGGTAA+AGG 0.443383 1_4:-54387359 Msa0170400:CDS
TTGAAGTAGTAATGGTTCTT+CGG 0.451189 1_4:+54384441 None:intergenic
TGATTTCATGTTCCTTTGGC+AGG 0.451994 1_4:+54387290 None:intergenic
CAGTAAAGGAGCTGTTTAAA+GGG 0.452750 1_4:-54387114 Msa0170400:CDS
AGTGTGATTTCATGTTCCTT+TGG 0.456196 1_4:+54387286 None:intergenic
TTATAGTAATAAATTTCATT+GGG 0.460815 1_4:+54383881 None:intergenic
CGGTAAAGGAGCTGTTTAAA+GGG 0.463391 1_4:-54387345 Msa0170400:CDS
TCATCATGGTTTGGGGAGAA+TGG 0.465856 1_4:+54387262 None:intergenic
AGTGTGATTTCATGTTCCTT+TGG 0.466154 1_4:+54387055 None:intergenic
TTATGATGCCGATAATTTCA+GGG 0.467165 1_4:-54383732 Msa0170400:CDS
CAGTCGATCATCATGGTTTG+TGG 0.478715 1_4:+54387486 None:intergenic
TCAATCTCAAAATGGAGAAT+GGG 0.478788 1_4:-54384050 Msa0170400:CDS
TTCATGTTCCTTTGGCAGGA+AGG 0.481994 1_4:+54387294 None:intergenic
CATCACATATAACGAGCCTT+AGG 0.496109 1_4:+54383858 None:intergenic
CCGGTGTATGCAAGAATTGG+AGG 0.497149 1_4:-54384513 Msa0170400:CDS
TGGTTCTTCGGATGCAAAGA+AGG 0.503168 1_4:+54384453 None:intergenic
CCCATTTACAAGAACAAAGA+AGG 0.506909 1_4:-54384194 Msa0170400:CDS
TGATTTCATGTTCCTTTGGT+AGG 0.507651 1_4:+54387059 None:intergenic
GTCACTTTAAAGGCCAACAT+AGG 0.508490 1_4:-54383956 Msa0170400:CDS
CATGGACCTTGAAGTAGTAA+TGG 0.508755 1_4:+54384370 None:intergenic
TCACTTTAAAGGCCAACATA+GGG 0.509233 1_4:-54383955 Msa0170400:CDS
ACTGAGCAATCCGAACTTGT+GGG 0.511461 1_4:-54383696 Msa0170400:CDS
AAATTTATTACTATAACCTA+AGG 0.513173 1_4:-54383874 Msa0170400:CDS
CGTGATCCCTTGCCACGACA+TGG 0.533777 1_4:+54384415 None:intergenic
TATAATCGTTAATTCCGGTA+GGG 0.534208 1_4:+54384161 None:intergenic
GATCAAAGAATTGATATAAG+AGG 0.536309 1_4:-54387379 Msa0170400:CDS
CCCAAACCATGATGATCGAC+TGG 0.536475 1_4:-54387254 Msa0170400:CDS
ATGCTCAGAAGAATTTGTCT+TGG 0.539972 1_4:-54384104 Msa0170400:CDS
TACTGAGCAATCCGAACTTG+TGG 0.540998 1_4:-54383697 Msa0170400:CDS
AATGGAAGATAAAGGGGAAG+TGG 0.542814 1_4:-54384014 Msa0170400:CDS
CATGATCCCTTGCCACGACA+TGG 0.542877 1_4:+54384352 None:intergenic
CATGGACCTTGAAGTAGTAA+TGG 0.552113 1_4:+54384433 None:intergenic
TCAAGGTCCATGTCGTGGCA+AGG 0.558917 1_4:-54384359 Msa0170400:CDS
TCAAGGTCCATGTCGTGGCA+AGG 0.558917 1_4:-54384422 Msa0170400:CDS
CAAGGTCCATGTCGTGGCAA+GGG 0.560575 1_4:-54384358 Msa0170400:CDS
CAAGGTCCATGTCGTGGCAA+GGG 0.560575 1_4:-54384421 Msa0170400:CDS
ATAATCGTTAATTCCGGTAG+GGG 0.560868 1_4:+54384162 None:intergenic
CCTGAACAGAGGAAGAACCT+TGG 0.561657 1_4:+54386051 None:intergenic
TATAAGAGGTGTCGTGGCAG+CGG 0.574258 1_4:-54387365 Msa0170400:CDS
TCCAACAATGGAAGATAAAG+GGG 0.575396 1_4:-54384020 Msa0170400:CDS
CGGTGTATGCAAGAATTGGA+GGG 0.575557 1_4:-54384512 Msa0170400:CDS
AAACTTCTCCAAACCGTCCA+AGG 0.575588 1_4:-54386068 Msa0170400:CDS
CAAGCTGAGTACCCCTATGT+TGG 0.581306 1_4:+54383943 None:intergenic
AAGGAAATCGATACCCCTAC+CGG 0.582838 1_4:-54384175 Msa0170400:CDS
AACAGAGGAAGAACCTTGGA+CGG 0.585223 1_4:+54386055 None:intergenic
TGTGATGGCTGGGAGTGAAA+AGG 0.588761 1_4:+54386101 None:intergenic
TTATAATCGTTAATTCCGGT+AGG 0.594124 1_4:+54384160 None:intergenic
GGCTGGGAGTGAAAAGGCAA+TGG 0.599173 1_4:+54386107 None:intergenic
GCATTTGTCAAAAGCAAGCA+AGG 0.600217 1_4:+54384123 None:intergenic
AATTGATATAAGAGGTGTCG+TGG 0.601164 1_4:-54387140 Msa0170400:CDS
CACTTTAAAGGCCAACATAG+GGG 0.601541 1_4:-54383954 Msa0170400:CDS
AATTGATATAAGAGGTGTCG+TGG 0.607984 1_4:-54387371 Msa0170400:CDS
ATTCAACACCTTCCTGCCAA+AGG 0.618562 1_4:-54387302 Msa0170400:CDS
TTTGATAGTATCTCAATTAG+GGG 0.624237 1_4:-54385876 Msa0170400:CDS
GATATGTCAGTCGATCATCA+TGG 0.627422 1_4:+54387479 None:intergenic
CTGAGCAATCCGAACTTGTG+GGG 0.628405 1_4:-54383695 Msa0170400:CDS
GATATGCCAGTCGATCATCA+TGG 0.639088 1_4:+54387248 None:intergenic
CAGTCGATCATCATGGTTTG+GGG 0.647099 1_4:+54387255 None:intergenic
CTACTTCAAGGTCCATGTCG+TGG 0.651161 1_4:-54384364 Msa0170400:CDS
CTACTTCAAGGTCCATGTCG+TGG 0.651161 1_4:-54384427 Msa0170400:CDS
CCTCCAATTCTTGCATACAC+CGG 0.651637 1_4:+54384513 None:intergenic
ATTCAACACCTTCCTACCAA+AGG 0.654326 1_4:-54387071 Msa0170400:CDS
AAGATGTAGTGTGTATTCAT+GGG 0.671832 1_4:-54383630 Msa0170400:CDS
GCGAAACTGAACCTGAACAG+AGG 0.696156 1_4:+54386040 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATAGTAATAAATTTCATT+GGG + chr1_4:54387129-54387148 None:intergenic 10.0%
!! TGTTAAAAGAAAAAGAAAAT+GGG + chr1_4:54384551-54384570 None:intergenic 15.0%
!! TTGTTAAAAGAAAAAGAAAA+TGG + chr1_4:54384552-54384571 None:intergenic 15.0%
!! AACAAAAACAAAAAATGAAA+AGG + chr1_4:54386034-54386053 None:intergenic 15.0%
!!! TTTTCATTTTTTGTTTTTGT+TGG - chr1_4:54386033-54386052 Msa0170400:intron 15.0%
!!! TTTCATTTTTTGTTTTTGTT+GGG - chr1_4:54386034-54386053 Msa0170400:intron 15.0%
!! TGACAACATATATTATATTA+TGG + chr1_4:54387035-54387054 None:intergenic 15.0%
!! GTTATAGTAATAAATTTCAT+TGG + chr1_4:54387130-54387149 None:intergenic 15.0%
!! AAATTTATTACTATAACCTA+AGG - chr1_4:54387133-54387152 Msa0170400:CDS 15.0%
!! TTTCAATTTAACATAATTCA+TGG + chr1_4:54387232-54387251 None:intergenic 15.0%
!! TTATGTTAAATTGAAAACTT+TGG - chr1_4:54387236-54387255 Msa0170400:CDS 15.0%
!! TATGTTAAATTGAAAACTTT+GGG - chr1_4:54387237-54387256 Msa0170400:CDS 15.0%
!! GATCAAAAAATTGATATAAG+AGG - chr1_4:54383859-54383878 Msa0170400:CDS 20.0%
!!! ATGATTTTCTGCAATTTTTT+TGG - chr1_4:54384039-54384058 Msa0170400:CDS 20.0%
!!! TATCTTTGGAGTATTAAAAT+AGG - chr1_4:54384351-54384370 Msa0170400:CDS 20.0%
!!! ATCTTTGGAGTATTAAAATA+GGG - chr1_4:54384352-54384371 Msa0170400:CDS 20.0%
!! TTATTTAATTGTAGAGTTTC+TGG + chr1_4:54384448-54384467 None:intergenic 20.0%
!! GTTAAAAGAAAAAGAAAATG+GGG + chr1_4:54384550-54384569 None:intergenic 20.0%
!! ATATTCAGCTTGTATTTATT+TGG - chr1_4:54384587-54384606 Msa0170400:intron 20.0%
!! TATTTATTCTTTAGGCTTAA+TGG + chr1_4:54384621-54384640 None:intergenic 20.0%
!!! TTCTCTTTATGTTTTGTTTT+AGG - chr1_4:54385069-54385088 Msa0170400:intron 20.0%
!!! TATTTGATAGTATCTCAATT+AGG - chr1_4:54385129-54385148 Msa0170400:intron 20.0%
!!! ATTTGATAGTATCTCAATTA+GGG - chr1_4:54385130-54385149 Msa0170400:intron 20.0%
!!! TTCATTTTTTGTTTTTGTTG+GGG - chr1_4:54386035-54386054 Msa0170400:intron 20.0%
!!! AATTTTTGTGAGGATAAAAA+TGG - chr1_4:54386113-54386132 Msa0170400:CDS 20.0%
!!! TATTTTTGTTTGGAGAAAAT+GGG - chr1_4:54386240-54386259 Msa0170400:intron 20.0%
!!! AATTTCTCCAGTAAATTTTA+TGG + chr1_4:54386525-54386544 None:intergenic 20.0%
!! ACAATTATAATCGTTAATTC+CGG + chr1_4:54386854-54386873 None:intergenic 20.0%
!!! AATATATGTTGTCACTTTAA+AGG - chr1_4:54387041-54387060 Msa0170400:CDS 20.0%
!! AAAGTTGCAAAAATGTATTA+CGG - chr1_4:54387407-54387426 Msa0170400:CDS 20.0%
!!! AAATGTTTTGATGAGTTTCA+AGG - chr1_4:54383606-54383625 Msa0170400:CDS 25.0%
! GATCAAAGAATTGATATAAG+AGG - chr1_4:54383628-54383647 Msa0170400:CDS 25.0%
!!! AGGGCATACAAATTTAATTT+TGG - chr1_4:54383681-54383700 Msa0170400:CDS 25.0%
!!! AGGGCATACATATTTAATTT+TGG - chr1_4:54383912-54383931 Msa0170400:CDS 25.0%
!!! GGGCATACATATTTAATTTT+GGG - chr1_4:54383913-54383932 Msa0170400:CDS 25.0%
! AATGTCATATATACAACTAG+AGG + chr1_4:54384159-54384178 None:intergenic 25.0%
!! ATCAAATTAGTCTCTAACTT+AGG + chr1_4:54384221-54384240 None:intergenic 25.0%
! AGTTAGAGACTAATTTGATT+AGG - chr1_4:54384222-54384241 Msa0170400:CDS 25.0%
! CTTAATCCATCAATTAGTTA+TGG + chr1_4:54384252-54384271 None:intergenic 25.0%
!!! TCTTTGGAGTATTAAAATAG+GGG - chr1_4:54384353-54384372 Msa0170400:CDS 25.0%
! AATACAAGCTGAATATAATG+AGG + chr1_4:54384583-54384602 None:intergenic 25.0%
! CTCAAAACAGTTTAAATTTG+GGG + chr1_4:54384823-54384842 None:intergenic 25.0%
! CCTCAAAACAGTTTAAATTT+GGG + chr1_4:54384824-54384843 None:intergenic 25.0%
!!! CCAAATTTAAACTGTTTTGA+GGG - chr1_4:54384822-54384841 Msa0170400:intron 25.0%
!! GTTTTTCTTTCTTTCAGTTA+GGG - chr1_4:54385091-54385110 Msa0170400:intron 25.0%
!! TTTTTCTTTCTTTCAGTTAG+GGG - chr1_4:54385092-54385111 Msa0170400:intron 25.0%
!! TTTGATAGTATCTCAATTAG+GGG - chr1_4:54385131-54385150 Msa0170400:intron 25.0%
! AGTAAGTTTAGTAAACCTAT+CGG + chr1_4:54385329-54385348 None:intergenic 25.0%
! AGAAAATCACATCAAGTAAA+TGG + chr1_4:54385429-54385448 None:intergenic 25.0%
!! ATTTACTTGATGTGATTTTC+TGG - chr1_4:54385428-54385447 Msa0170400:intron 25.0%
! TCCAAAAAATGATCAAATAG+CGG + chr1_4:54385651-54385670 None:intergenic 25.0%
! AAAAAGAGAACAAAGAACAT+AGG - chr1_4:54385915-54385934 Msa0170400:intron 25.0%
! AGGATTAAAGACATATGAAA+AGG + chr1_4:54386014-54386033 None:intergenic 25.0%
!! CTAGAATCTTAATTTTTGTG+AGG - chr1_4:54386103-54386122 Msa0170400:CDS 25.0%
!!! ATCTATTGTTTGCTATACAT+TGG - chr1_4:54386138-54386157 Msa0170400:intron 25.0%
! CACGTATTTGTATTATATGA+TGG - chr1_4:54386213-54386232 Msa0170400:intron 25.0%
!!! GTATTTTTGTTTGGAGAAAA+TGG - chr1_4:54386239-54386258 Msa0170400:intron 25.0%
! CTGTTTATCTGATAATATCA+AGG + chr1_4:54386295-54386314 None:intergenic 25.0%
! AATTTACTGGAGAAATTTCT+TGG - chr1_4:54386528-54386547 Msa0170400:intron 25.0%
!! TCTTCAAGCAATAAACTTTT+TGG + chr1_4:54386702-54386721 None:intergenic 25.0%
!! ATCTATGTTACATTTTCTAG+CGG - chr1_4:54386748-54386767 Msa0170400:intron 25.0%
CAACTAGAGGACTAATATTA+TGG + chr1_4:54384146-54384165 None:intergenic 30.0%
! GGTTTTCCATAACTAATTGA+TGG - chr1_4:54384243-54384262 Msa0170400:CDS 30.0%
! TTCATGCATTTTATGTCCTT+GGG - chr1_4:54384376-54384395 Msa0170400:CDS 30.0%
AGTTGTAAGACAAGTGTTAT+CGG + chr1_4:54384415-54384434 None:intergenic 30.0%
! CTTTAGGCTTAATGGATAAT+GGG + chr1_4:54384613-54384632 None:intergenic 30.0%
! TCTTTAGGCTTAATGGATAA+TGG + chr1_4:54384614-54384633 None:intergenic 30.0%
AGCCTAAAGAATAAATACCA+TGG - chr1_4:54384624-54384643 Msa0170400:intron 30.0%
GCCTAAAGAATAAATACCAT+GGG - chr1_4:54384625-54384644 Msa0170400:intron 30.0%
TCCCATGGTATTTATTCTTT+AGG + chr1_4:54384629-54384648 None:intergenic 30.0%
TTCACATTTAAACACAGTAG+CGG + chr1_4:54384798-54384817 None:intergenic 30.0%
! CCCAAATTTAAACTGTTTTG+AGG - chr1_4:54384821-54384840 Msa0170400:intron 30.0%
CCCTCAAAACAGTTTAAATT+TGG + chr1_4:54384825-54384844 None:intergenic 30.0%
!!! AAACTGTTTTGAGGGTAATT+TGG - chr1_4:54384830-54384849 Msa0170400:intron 30.0%
!!! AACTGTTTTGAGGGTAATTT+GGG - chr1_4:54384831-54384850 Msa0170400:intron 30.0%
!!! AGTTTTGTTTTGCTTTGTGA+TGG + chr1_4:54384924-54384943 None:intergenic 30.0%
! GGTTTTTCTTTCTTTCAGTT+AGG - chr1_4:54385090-54385109 Msa0170400:intron 30.0%
AGAAAAGATCGGATAATGTT+TGG + chr1_4:54385208-54385227 None:intergenic 30.0%
! TTATCCGATCTTTTCTTGTT+TGG - chr1_4:54385212-54385231 Msa0170400:intron 30.0%
!! TATCCGATCTTTTCTTGTTT+GGG - chr1_4:54385213-54385232 Msa0170400:intron 30.0%
CAAAATTCAAACCAAACTGA+AGG - chr1_4:54385237-54385256 Msa0170400:intron 30.0%
AATCCATGCATGATTAAAGT+GGG + chr1_4:54385355-54385374 None:intergenic 30.0%
TGCTCTTTGTAACCTTATTA+TGG + chr1_4:54385475-54385494 None:intergenic 30.0%
CATCATACTACACACTTTAT+AGG + chr1_4:54385500-54385519 None:intergenic 30.0%
!! GTTGATTTGTTCAACAAGTT+AGG - chr1_4:54385544-54385563 Msa0170400:intron 30.0%
!!! ACCGCTATTTGATCATTTTT+TGG - chr1_4:54385647-54385666 Msa0170400:intron 30.0%
GGATTTGAATGTAATGTTTG+TGG + chr1_4:54385778-54385797 None:intergenic 30.0%
TTTATTTCCCTGACATTTGT+TGG + chr1_4:54385806-54385825 None:intergenic 30.0%
! TGAGACTTGTGAAATTTTGT+TGG + chr1_4:54385854-54385873 None:intergenic 30.0%
!!! TTTTTAGTTCCTCTGTTCTT+TGG + chr1_4:54385899-54385918 None:intergenic 30.0%
TATGAAAAGGTCAATTAGTG+AGG + chr1_4:54386001-54386020 None:intergenic 30.0%
!! AGCTGAAAGCACTAAATATT+AGG + chr1_4:54386085-54386104 None:intergenic 30.0%
GGCAAAATAAACTATTTGCA+TGG - chr1_4:54386176-54386195 Msa0170400:intron 30.0%
ACTATTTGCATGGAAAAAAG+AGG - chr1_4:54386186-54386205 Msa0170400:intron 30.0%
!! TGATGGTCTGTATTTTTGTT+TGG - chr1_4:54386230-54386249 Msa0170400:intron 30.0%
CTGATAATATCAAGGAAATG+AGG + chr1_4:54386287-54386306 None:intergenic 30.0%
ACAACAACAACAACATTGTT+AGG + chr1_4:54386368-54386387 None:intergenic 30.0%
ATGCAAAGGAAAAAGATTGA+AGG - chr1_4:54386389-54386408 Msa0170400:intron 30.0%
! GGGTTTTCCATAAAATTTAC+TGG - chr1_4:54386515-54386534 Msa0170400:intron 30.0%
TTGAAGTAGTAATGGTTCTT+CGG + chr1_4:54386569-54386588 None:intergenic 30.0%
GAAAAACCATTACTACTTCA+AGG - chr1_4:54386631-54386650 Msa0170400:intron 30.0%
TTGGTTGGATATGCTAATAA+AGG + chr1_4:54386683-54386702 None:intergenic 30.0%
!! CAAGCAATAAACTTTTTGGT+TGG + chr1_4:54386698-54386717 None:intergenic 30.0%
AGTTTATTGCTTGAAGATGA+TGG - chr1_4:54386705-54386724 Msa0170400:intron 30.0%
!! TTCTAGCGGATATTTTATCA+TGG - chr1_4:54386762-54386781 Msa0170400:intron 30.0%
CCTTCTTTGTTCTTGTAAAT+GGG + chr1_4:54386816-54386835 None:intergenic 30.0%
TCCTTCTTTGTTCTTGTAAA+TGG + chr1_4:54386817-54386836 None:intergenic 30.0%
TATAATCGTTAATTCCGGTA+GGG + chr1_4:54386849-54386868 None:intergenic 30.0%
TTATAATCGTTAATTCCGGT+AGG + chr1_4:54386850-54386869 None:intergenic 30.0%
CATGTCTATCAATCTCAAAA+TGG - chr1_4:54386949-54386968 Msa0170400:intron 30.0%
ATCAATCTCAAAATGGAGAA+TGG - chr1_4:54386956-54386975 Msa0170400:intron 30.0%
TCAATCTCAAAATGGAGAAT+GGG - chr1_4:54386957-54386976 Msa0170400:intron 30.0%
!!! ATGGGTTTTTTATCCAACAA+TGG - chr1_4:54386975-54386994 Msa0170400:intron 30.0%
TATCCAACAATGGAAGATAA+AGG - chr1_4:54386985-54387004 Msa0170400:intron 30.0%
ATCCAACAATGGAAGATAAA+GGG - chr1_4:54386986-54387005 Msa0170400:intron 30.0%
! TATATGTGATGAGCAACTTT+TGG - chr1_4:54387161-54387180 Msa0170400:CDS 30.0%
AAGAATGTGTTCAAGATAGA+AGG - chr1_4:54387201-54387220 Msa0170400:CDS 30.0%
TTTATGATGCCGATAATTTC+AGG - chr1_4:54387274-54387293 Msa0170400:CDS 30.0%
TTATGATGCCGATAATTTCA+GGG - chr1_4:54387275-54387294 Msa0170400:CDS 30.0%
ACAATTTGCCCTGAAATTAT+CGG + chr1_4:54387286-54387305 None:intergenic 30.0%
AAGATGTAGTGTGTATTCAT+GGG - chr1_4:54387377-54387396 Msa0170400:CDS 30.0%
!! AATTGATATAAGAGGTGTCG+TGG - chr1_4:54383636-54383655 Msa0170400:CDS 35.0%
AGTGTGATTTCATGTTCCTT+TGG + chr1_4:54383724-54383743 None:intergenic 35.0%
AAACGTTCTGATGAGTTTCA+AGG - chr1_4:54383837-54383856 Msa0170400:CDS 35.0%
!! AATTGATATAAGAGGTGTCG+TGG - chr1_4:54383636-54383655 Msa0170400:CDS 35.0%
CAGTAAAGGAGCTGTTTAAA+GGG - chr1_4:54383893-54383912 Msa0170400:CDS 35.0%
TGATTTCATGTTCCTTTGGT+AGG + chr1_4:54383951-54383970 None:intergenic 35.0%
AGTGTGATTTCATGTTCCTT+TGG + chr1_4:54383724-54383743 None:intergenic 35.0%
!! CCATTCATCTCAAGTTGAAA+TGG + chr1_4:54383979-54383998 None:intergenic 35.0%
CCATTTCAACTTGAGATGAA+TGG - chr1_4:54383976-54383995 Msa0170400:CDS 35.0%
AACCAAAAATCATGGCATTG+CGG + chr1_4:54384111-54384130 None:intergenic 35.0%
TGGCAACAAACCAAAAATCA+TGG + chr1_4:54384119-54384138 None:intergenic 35.0%
! AGGACTAATATTATGGTGGA+TGG + chr1_4:54384139-54384158 None:intergenic 35.0%
CTAGAGGACTAATATTATGG+TGG + chr1_4:54384143-54384162 None:intergenic 35.0%
CTTTGTTAGATTGAGACAGA+CGG - chr1_4:54384192-54384211 Msa0170400:CDS 35.0%
GCTGATGAAGTCTATATCTT+TGG - chr1_4:54384337-54384356 Msa0170400:CDS 35.0%
! GTTCATGCATTTTATGTCCT+TGG - chr1_4:54384375-54384394 Msa0170400:CDS 35.0%
ACAAGCTGAATATAATGAGG+AGG + chr1_4:54384580-54384599 None:intergenic 35.0%
! TGAGGGTAATTTGGGTATTT+TGG - chr1_4:54384839-54384858 Msa0170400:intron 35.0%
!! ATGTTTGGTTTGGTTTGGTT+TGG + chr1_4:54385193-54385212 None:intergenic 35.0%
!! GGATAATGTTTGGTTTGGTT+TGG + chr1_4:54385198-54385217 None:intergenic 35.0%
AGATCGGATAATGTTTGGTT+TGG + chr1_4:54385203-54385222 None:intergenic 35.0%
TGACCCAAACAAGAAAAGAT+CGG + chr1_4:54385219-54385238 None:intergenic 35.0%
!! GAAAACGAGTTGGATTAGTT+TGG - chr1_4:54385259-54385278 Msa0170400:intron 35.0%
!! AGTTGGATTAGTTTGGTTCT+TGG - chr1_4:54385266-54385285 Msa0170400:intron 35.0%
!! GGTTTGGTTTATCGGTTTAT+TGG + chr1_4:54385308-54385327 None:intergenic 35.0%
!! AACCTATCGGTTTGGTTTAT+CGG + chr1_4:54385316-54385335 None:intergenic 35.0%
! GTTTAGTAAACCTATCGGTT+TGG + chr1_4:54385324-54385343 None:intergenic 35.0%
GAATCCATGCATGATTAAAG+TGG + chr1_4:54385356-54385375 None:intergenic 35.0%
GTGAATTCTTGCTTCAATCT+TGG - chr1_4:54385383-54385402 Msa0170400:intron 35.0%
!!! GTGATTTTCTGGAGTTGTTT+TGG - chr1_4:54385439-54385458 Msa0170400:intron 35.0%
!!! GGAGTTGTTTTGGAATGATA+TGG - chr1_4:54385449-54385468 Msa0170400:intron 35.0%
GGAATGATATGGCCATAATA+AGG - chr1_4:54385460-54385479 Msa0170400:intron 35.0%
TCCAAATACCAACAAATGTC+AGG - chr1_4:54385795-54385814 Msa0170400:intron 35.0%
CCAAATACCAACAAATGTCA+GGG - chr1_4:54385796-54385815 Msa0170400:intron 35.0%
!!! TTTTGTTTTTGTTGGGGTGT+AGG - chr1_4:54386041-54386060 Msa0170400:intron 35.0%
!!! TTTGTTTTTGTTGGGGTGTA+GGG - chr1_4:54386042-54386061 Msa0170400:intron 35.0%
! TTGTTGTTGTTGTGATGCAA+AGG - chr1_4:54386375-54386394 Msa0170400:intron 35.0%
AGAGAATTGTGAACTTCCAT+CGG - chr1_4:54386416-54386435 Msa0170400:intron 35.0%
GAAGAACCATTACTACTTCA+AGG - chr1_4:54386568-54386587 Msa0170400:intron 35.0%
!! TGGTTTTTCAGATGCAAAGA+AGG + chr1_4:54386620-54386639 None:intergenic 35.0%
! CGGATATTTTATCATGGTGA+GGG - chr1_4:54386768-54386787 Msa0170400:intron 35.0%
CCCATTTACAAGAACAAAGA+AGG - chr1_4:54386813-54386832 Msa0170400:intron 35.0%
ATAATCGTTAATTCCGGTAG+GGG + chr1_4:54386848-54386867 None:intergenic 35.0%
ATGCTCAGAAGAATTTGTCT+TGG - chr1_4:54386903-54386922 Msa0170400:intron 35.0%
TCCAACAATGGAAGATAAAG+GGG - chr1_4:54386987-54387006 Msa0170400:intron 35.0%
! TCACTTTAAAGGCCAACATA+GGG - chr1_4:54387052-54387071 Msa0170400:CDS 35.0%
!! AGCAACTTTTGGTGGTTAAT+AGG - chr1_4:54387172-54387191 Msa0170400:CDS 35.0%
CAAGATGTAGTGTGTATTCA+TGG - chr1_4:54387376-54387395 Msa0170400:CDS 35.0%
ATTACGGTTTCAACTCTTGA+TGG - chr1_4:54387423-54387442 Msa0170400:CDS 35.0%
GATATGTCAGTCGATCATCA+TGG + chr1_4:54383531-54383550 None:intergenic 40.0%
CGGTAAAGGAGCTGTTTAAA+GGG - chr1_4:54383662-54383681 Msa0170400:CDS 40.0%
TGATTTCATGTTCCTTTGGC+AGG + chr1_4:54383720-54383739 None:intergenic 40.0%
GCAGTAAAGGAGCTGTTTAA+AGG - chr1_4:54383892-54383911 Msa0170400:CDS 40.0%
ATTCAACACCTTCCTACCAA+AGG - chr1_4:54383936-54383955 Msa0170400:CDS 40.0%
TTCATGTTCCTTTGGTAGGA+AGG + chr1_4:54383947-54383966 None:intergenic 40.0%
TGTAAGACAAGTGTTATCGG+TGG + chr1_4:54384412-54384431 None:intergenic 40.0%
AAACAAAATGGTTGCACGGA+GGG + chr1_4:54384522-54384541 None:intergenic 40.0%
AGCCAAACAAAATGGTTGCA+CGG + chr1_4:54384526-54384545 None:intergenic 40.0%
AATGGGGAAGCCAAACAAAA+TGG + chr1_4:54384534-54384553 None:intergenic 40.0%
!! TAAACACAGTAGCGGATTAG+AGG + chr1_4:54384790-54384809 None:intergenic 40.0%
! TGTTTTGCTTTGTGATGGCT+GGG + chr1_4:54384919-54384938 None:intergenic 40.0%
!! TTGTTTTGCTTTGTGATGGC+TGG + chr1_4:54384920-54384939 None:intergenic 40.0%
TGAGAGAGAAAGAAGAGAGA+TGG + chr1_4:54384994-54385013 None:intergenic 40.0%
GAGAGAGCAAAGAAAAGAGA+TGG + chr1_4:54385042-54385061 None:intergenic 40.0%
! ATCTCAATTAGGGGTGTAAG+TGG - chr1_4:54385140-54385159 Msa0170400:intron 40.0%
! AATTAGGGGTGTAAGTGGTT+TGG - chr1_4:54385145-54385164 Msa0170400:intron 40.0%
!!! GGTTTGGTTTTGGAGTTCAT+CGG + chr1_4:54385177-54385196 None:intergenic 40.0%
!!! GGTTTGGTTTGGTTTGGTTT+TGG + chr1_4:54385187-54385206 None:intergenic 40.0%
! CAACTCGTTTTCCTTCAGTT+TGG + chr1_4:54385251-54385270 None:intergenic 40.0%
CAAACTGAAGGAAAACGAGT+TGG - chr1_4:54385249-54385268 Msa0170400:intron 40.0%
AACCGATAAACCAAACCGAT+AGG - chr1_4:54385311-54385330 Msa0170400:intron 40.0%
AAGCCCACTTTAATCATGCA+TGG - chr1_4:54385349-54385368 Msa0170400:intron 40.0%
TGATGCATAGTAGTCAATCC+CGG - chr1_4:54385575-54385594 Msa0170400:intron 40.0%
!! TATACGCCATCCCACTTTTA+GGG + chr1_4:54385619-54385638 None:intergenic 40.0%
! CCCTGACATTTGTTGGTATT+TGG + chr1_4:54385799-54385818 None:intergenic 40.0%
TCCAAAAGACCAAAGAACAG+AGG - chr1_4:54385887-54385906 Msa0170400:CDS 40.0%
!! TCCTCTGTTCTTTGGTCTTT+TGG + chr1_4:54385891-54385910 None:intergenic 40.0%
TAGGAAGATAGATTCCGTAG+CGG - chr1_4:54385934-54385953 Msa0170400:intron 40.0%
!!! TTGTTTTTGTTGGGGTGTAG+GGG - chr1_4:54386043-54386062 Msa0170400:intron 40.0%
CATTGGCATGATCACGAAAA+TGG - chr1_4:54386155-54386174 Msa0170400:intron 40.0%
ATGATATCAAGCACATCCGA+TGG + chr1_4:54386435-54386454 None:intergenic 40.0%
ACGATCTCTTTCAGATTTGC+CGG - chr1_4:54386475-54386494 Msa0170400:intron 40.0%
CATGGACCTTGAAGTAGTAA+TGG + chr1_4:54386577-54386596 None:intergenic 40.0%
CATGGACCTTGAAGTAGTAA+TGG + chr1_4:54386577-54386596 None:intergenic 40.0%
! GCGGATATTTTATCATGGTG+AGG - chr1_4:54386767-54386786 Msa0170400:intron 40.0%
GCATTTGTCAAAAGCAAGCA+AGG + chr1_4:54386887-54386906 None:intergenic 40.0%
TCCCCTTTATCTTCCATTGT+TGG + chr1_4:54386991-54387010 None:intergenic 40.0%
AATGGAAGATAAAGGGGAAG+TGG - chr1_4:54386993-54387012 Msa0170400:intron 40.0%
! GTCACTTTAAAGGCCAACAT+AGG - chr1_4:54387051-54387070 Msa0170400:CDS 40.0%
! CACTTTAAAGGCCAACATAG+GGG - chr1_4:54387053-54387072 Msa0170400:CDS 40.0%
CATCACATATAACGAGCCTT+AGG + chr1_4:54387152-54387171 None:intergenic 40.0%
! ATGTGATGAGCAACTTTTGG+TGG - chr1_4:54387164-54387183 Msa0170400:CDS 40.0%
CAAGTTCTCGCATGTTTGAT+TGG - chr1_4:54387448-54387467 Msa0170400:CDS 40.0%
CAGTCGATCATCATGGTTTG+TGG + chr1_4:54383524-54383543 None:intergenic 45.0%
GCGGTAAAGGAGCTGTTTAA+AGG - chr1_4:54383661-54383680 Msa0170400:CDS 45.0%
ATTCAACACCTTCCTGCCAA+AGG - chr1_4:54383705-54383724 Msa0170400:CDS 45.0%
TTCATGTTCCTTTGGCAGGA+AGG + chr1_4:54383716-54383735 None:intergenic 45.0%
! TCATCATGGTTTGGGGAGAA+TGG + chr1_4:54383748-54383767 None:intergenic 45.0%
CAGTCGATCATCATGGTTTG+GGG + chr1_4:54383524-54383543 None:intergenic 45.0%
CCAGTCGATCATCATGGTTT+GGG + chr1_4:54383756-54383775 None:intergenic 45.0%
GATATGCCAGTCGATCATCA+TGG + chr1_4:54383762-54383781 None:intergenic 45.0%
CAAACAAAATGGTTGCACGG+AGG + chr1_4:54384523-54384542 None:intergenic 45.0%
! CTCCGTGCAACCATTTTGTT+TGG - chr1_4:54384521-54384540 Msa0170400:CDS 45.0%
GCGCTATTAACTACCTAGGT+TGG + chr1_4:54384655-54384674 None:intergenic 45.0%
TAGCGCGCTATTAACTACCT+AGG + chr1_4:54384659-54384678 None:intergenic 45.0%
TATTGCGAACTGCTCTGCTA+TGG + chr1_4:54384718-54384737 None:intergenic 45.0%
GGAAGCTATAGCATCTAGAG+CGG + chr1_4:54384745-54384764 None:intergenic 45.0%
CTCTAGATGCTATAGCTTCC+CGG - chr1_4:54384745-54384764 Msa0170400:intron 45.0%
TCTAGATGCTATAGCTTCCC+GGG - chr1_4:54384746-54384765 Msa0170400:intron 45.0%
GAAGTGTGAGAAGAGGGTTA+TGG + chr1_4:54384870-54384889 None:intergenic 45.0%
GTTGCTGAAGTGTGAGAAGA+GGG + chr1_4:54384876-54384895 None:intergenic 45.0%
AGTTGCTGAAGTGTGAGAAG+AGG + chr1_4:54384877-54384896 None:intergenic 45.0%
AAACTTCTCCAAACCGTCCA+AGG - chr1_4:54384939-54384958 Msa0170400:intron 45.0%
AACAGAGGAAGAACCTTGGA+CGG + chr1_4:54384955-54384974 None:intergenic 45.0%
TTTCAGTTAGGGGTGAGCTT+TGG - chr1_4:54385102-54385121 Msa0170400:intron 45.0%
!! CTATACGCCATCCCACTTTT+AGG + chr1_4:54385620-54385639 None:intergenic 45.0%
CCACGTAATTTCCCTACAAG+CGG + chr1_4:54385967-54385986 None:intergenic 45.0%
TTGCCGGTGTATGCAAGAAT+TGG - chr1_4:54386491-54386510 Msa0170400:intron 45.0%
CCTCCAATTCTTGCATACAC+CGG + chr1_4:54386497-54386516 None:intergenic 45.0%
CGGTGTATGCAAGAATTGGA+GGG - chr1_4:54386495-54386514 Msa0170400:intron 45.0%
TGGTTCTTCGGATGCAAAGA+AGG + chr1_4:54386557-54386576 None:intergenic 45.0%
AAGGAAATCGATACCCCTAC+CGG - chr1_4:54386832-54386851 Msa0170400:intron 45.0%
TACTGAGCAATCCGAACTTG+TGG - chr1_4:54387310-54387329 Msa0170400:CDS 45.0%
ACTGAGCAATCCGAACTTGT+GGG - chr1_4:54387311-54387330 Msa0170400:CDS 45.0%
CTCTCATATCCCCACAAGTT+CGG + chr1_4:54387324-54387343 None:intergenic 45.0%
!! TATAAGAGGTGTCGTGGCAG+CGG - chr1_4:54383642-54383661 Msa0170400:CDS 50.0%
CCCAAACCATGATGATCGAC+TGG - chr1_4:54383753-54383772 Msa0170400:CDS 50.0%
GCCAGTCGATCATCATGGTT+TGG + chr1_4:54383757-54383776 None:intergenic 50.0%
AATCATGGCATTGCGGCGTA+TGG + chr1_4:54384104-54384123 None:intergenic 50.0%
! CGCCGCAATGCCATGATTTT+TGG - chr1_4:54384106-54384125 Msa0170400:CDS 50.0%
TACCATGGGATGACCAACCT+AGG - chr1_4:54384639-54384658 Msa0170400:intron 50.0%
!! TACCTAGGTTGGTCATCCCA+TGG + chr1_4:54384644-54384663 None:intergenic 50.0%
TATAGCAGCGTAGCAGACGT+TGG - chr1_4:54384677-54384696 Msa0170400:intron 50.0%
TGCTATGGCTATAACCAGCG+CGG + chr1_4:54384703-54384722 None:intergenic 50.0%
AGCAGAGCAGTTCGCAATAG+CGG - chr1_4:54384719-54384738 Msa0170400:intron 50.0%
TGTGATGGCTGGGAGTGAAA+AGG + chr1_4:54384909-54384928 None:intergenic 50.0%
AGGAAGAACCTTGGACGGTT+TGG + chr1_4:54384950-54384969 None:intergenic 50.0%
CCTGAACAGAGGAAGAACCT+TGG + chr1_4:54384959-54384978 None:intergenic 50.0%
CCAAGGTTCTTCCTCTGTTC+AGG - chr1_4:54384956-54384975 Msa0170400:intron 50.0%
GCGAAACTGAACCTGAACAG+AGG + chr1_4:54384970-54384989 None:intergenic 50.0%
TAGTGGCCGACCCTAAAAGT+GGG - chr1_4:54385606-54385625 Msa0170400:intron 50.0%
AAAGTGGGATGGCGTATAGC+AGG - chr1_4:54385621-54385640 Msa0170400:intron 50.0%
CCGCTTGTAGGGAAATTACG+TGG - chr1_4:54385964-54385983 Msa0170400:intron 50.0%
CCGGTGTATGCAAGAATTGG+AGG - chr1_4:54386494-54386513 Msa0170400:intron 50.0%
CTACTTCAAGGTCCATGTCG+TGG - chr1_4:54386580-54386599 Msa0170400:intron 50.0%
CTACTTCAAGGTCCATGTCG+TGG - chr1_4:54386580-54386599 Msa0170400:intron 50.0%
! AGGGGAAGTGGTTGACTTTG+TGG - chr1_4:54387005-54387024 Msa0170400:intron 50.0%
CAAGCTGAGTACCCCTATGT+TGG + chr1_4:54387067-54387086 None:intergenic 50.0%
CTGAGCAATCCGAACTTGTG+GGG - chr1_4:54387312-54387331 Msa0170400:CDS 50.0%
! AGGTGTCGTGGCAGCAGTAA+AGG - chr1_4:54383879-54383898 Msa0170400:CDS 55.0%
TGCGGCGTATGGTAGAACGA+TGG + chr1_4:54384093-54384112 None:intergenic 55.0%
! CGGTGGACATTAGTCTCCCA+AGG + chr1_4:54384395-54384414 None:intergenic 55.0%
TGCTATAGCTTCCCGGGTAG+CGG - chr1_4:54384752-54384771 Msa0170400:intron 55.0%
GGCTGGGAGTGAAAAGGCAA+TGG + chr1_4:54384903-54384922 None:intergenic 55.0%
GTAGTGGCCGACCCTAAAAG+TGG - chr1_4:54385605-54385624 Msa0170400:intron 55.0%
!!! CGCCATCCCACTTTTAGGGT+CGG + chr1_4:54385615-54385634 None:intergenic 55.0%
CAAGCGGCAAGAAACCGCTA+CGG + chr1_4:54385951-54385970 None:intergenic 55.0%
! AGCGGTTTCTTGCCGCTTGT+AGG - chr1_4:54385952-54385971 Msa0170400:intron 55.0%
! GCGGTTTCTTGCCGCTTGTA+GGG - chr1_4:54385953-54385972 Msa0170400:intron 55.0%
TCAAGGTCCATGTCGTGGCA+AGG - chr1_4:54386585-54386604 Msa0170400:intron 55.0%
CAAGGTCCATGTCGTGGCAA+GGG - chr1_4:54386586-54386605 Msa0170400:intron 55.0%
TCAAGGTCCATGTCGTGGCA+AGG - chr1_4:54386585-54386604 Msa0170400:intron 55.0%
CAAGGTCCATGTCGTGGCAA+GGG - chr1_4:54386586-54386605 Msa0170400:intron 55.0%
CATGATCCCTTGCCACGACA+TGG + chr1_4:54386658-54386677 None:intergenic 55.0%
! AGGTGTCGTGGCAGCGGTAA+AGG - chr1_4:54383648-54383667 Msa0170400:CDS 60.0%
GCTATAGCTTCCCGGGTAGC+GGG - chr1_4:54384753-54384772 Msa0170400:intron 60.0%
CAATCCCGGACAGTGCGTAG+TGG - chr1_4:54385589-54385608 Msa0170400:intron 60.0%
GGCCGACCCTAAAAGTGGGA+TGG - chr1_4:54385610-54385629 Msa0170400:intron 60.0%
CGTGATCCCTTGCCACGACA+TGG + chr1_4:54386595-54386614 None:intergenic 60.0%
TGCTAACCTGCCCGCTACCC+GGG + chr1_4:54384766-54384785 None:intergenic 65.0%
CTGCTAACCTGCCCGCTACC+CGG + chr1_4:54384767-54384786 None:intergenic 65.0%
TCGGCCACTACGCACTGTCC+GGG + chr1_4:54385596-54385615 None:intergenic 65.0%
GTCGGCCACTACGCACTGTC+CGG + chr1_4:54385597-54385616 None:intergenic 65.0%
GTAGCAGACGTTGGCCGCGC+TGG - chr1_4:54384686-54384705 Msa0170400:intron 70.0%
!! TAGCTTCCCGGGTAGCGGGC+AGG - chr1_4:54384757-54384776 Msa0170400:intron 70.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 54383511 54387518 54383511 ID=Msa0170400;Name=Msa0170400
chr1_4 mRNA 54383511 54387518 54383511 ID=Msa0170400-mRNA-1;Parent=Msa0170400;Name=Msa0170400-mRNA-1;_AED=0.10;_eAED=0.10;_QI=0|0|0|0.5|1|1|4|0|549
chr1_4 exon 54387032 54387518 54387032 ID=Msa0170400-mRNA-1:exon:16237;Parent=Msa0170400-mRNA-1
chr1_4 exon 54386052 54386137 54386052 ID=Msa0170400-mRNA-1:exon:16236;Parent=Msa0170400-mRNA-1
chr1_4 exon 54385875 54385921 54385875 ID=Msa0170400-mRNA-1:exon:16235;Parent=Msa0170400-mRNA-1
chr1_4 exon 54383511 54384540 54383511 ID=Msa0170400-mRNA-1:exon:16234;Parent=Msa0170400-mRNA-1
chr1_4 CDS 54387032 54387518 54387032 ID=Msa0170400-mRNA-1:cds;Parent=Msa0170400-mRNA-1
chr1_4 CDS 54386052 54386137 54386052 ID=Msa0170400-mRNA-1:cds;Parent=Msa0170400-mRNA-1
chr1_4 CDS 54385875 54385921 54385875 ID=Msa0170400-mRNA-1:cds;Parent=Msa0170400-mRNA-1
chr1_4 CDS 54383511 54384540 54383511 ID=Msa0170400-mRNA-1:cds;Parent=Msa0170400-mRNA-1
Gene Sequence

>Msa0170400

ATGAAGCTCTCCACAAACCATGATGATCGACTGACATATCTTGCGACAGTCAAAGTTGCATTTCAACATAACATGATGAAGTATGATCAATTCTTAAATGTTTTGATGAGTTTCAAGGATCAAAGAATTGATATAAGAGGTGTCGTGGCAGCGGTAAAGGAGCTGTTTAAAGGGCATACAAATTTAATTTTGGCATTCAACACCTTCCTGCCAAAGGAACATGAAATCACACTTCCATTCTCCCCAAACCATGATGATCGACTGGCATATCTCGCGACAGTCAAAGTTGCATTTCAACATAACATGATGAAGTATGATCAATTCTTAAACGTTCTGATGAGTTTCAAGGATCAAAAAATTGATATAAGAGGTGTCGTGGCAGCAGTAAAGGAGCTGTTTAAAGGGCATACATATTTAATTTTGGGATTCAACACCTTCCTACCAAAGGAACATGAAATCACACTTCCATTTCAACTTGAGATGAATGCAACTGTTCCATTGCCTTTTCACTCCCAGCCATCACAAAGCAAAACAAAACTTCTCCAAACCGTCCAAGGTTCTTCCTCTGTTCAGTTAGGGGTGAGCTTTGGAAGCTATTTGATAGTATCTCAATTAGGGGTATTTGCCGGTGTATGCAAGAATTGGAGGGTTTTCCATAAAATTTACTGGAGAAATTTCTTGGAATCCTTCTTTGCATCCGAAGAACCATTACTACTTCAAGGTCCATGTCGTGGCAAGGGATCACGTTCCTTCTTTGCATCTGAAAAACCATTACTACTTCAAGGTCCATGTCGTGGCAAGGGATCATGTTCCTTTATTAGCATATCCAACCAAAAAGTTTATTGCTTGAAGATGATGGAATACTTCTTCAATCACTATATCTATGTTACATTTTCTAGCGGATATTTTATCATGGTGAGGGATGATGTATTTGTGCTAATCAACCCATTTACAAGAACAAAGAAGGAAATCGATACCCCTACCGGAATTAACGATTATAATTGTACTGATCGTGCCTTGCTTGCTTTTGACAAATGCTCAGAAGAATTTGTCTTGGTGATTTCAAGTCTAAGTCATTTGCATGTCTATCAATCTCAAAATGGAGAATGGGTTTTTTATCCAACAATGGAAGATAAAGGGGAAGTGGTTGACTTTGTGGTTTTCCATAATATAATATATGTTGTCACTTTAAAGGCCAACATAGGGGTACTCAGCTTGAACTCTGCAAATATTAAATTTCTAAAACTGAATAGTACTCCCAATGAAATTTATTACTATAACCTAAGGCTCGTTATATGTGATGAGCAACTTTTGGTGGTTAATAGGCAAATAAAGAATGTGTTCAAGATAGAAGGCTCAACCATGAATTATGTTAAATTGAAAACTTTGGGCGACATTGCACTATTTTATGATGCCGATAATTTCAGGGCAAATTGTCATGTACTGAGCAATCCGAACTTGTGGGGATATGAGAGCAACTCTGTGTATATCATCAATCAGTTTTCTACAAGATGTAGTGTGTATTCATGGGCTGATAAAAAGTTGCAAAAATGTATTACGGTTTCAACTCTTGATGGAACAAGTTCTCGCATGTTTGATTGGTGTATTAGATGTCTACGATATGAAATAGATTATTCTCTAGTTGAGTAG
Protein sequence

>Msa0170400

MKLSTNHDDRLTYLATVKVAFQHNMMKYDQFLNVLMSFKDQRIDIRGVVAAVKELFKGHTNLILAFNTFLPKEHEITLPFSPNHDDRLAYLATVKVAFQHNMMKYDQFLNVLMSFKDQKIDIRGVVAAVKELFKGHTYLILGFNTFLPKEHEITLPFQLEMNATVPLPFHSQPSQSKTKLLQTVQGSSSVQLGVSFGSYLIVSQLGVFAGVCKNWRVFHKIYWRNFLESFFASEEPLLLQGPCRGKGSRSFFASEKPLLLQGPCRGKGSCSFISISNQKVYCLKMMEYFFNHYIYVTFSSGYFIMVRDDVFVLINPFTRTKKEIDTPTGINDYNCTDRALLAFDKCSEEFVLVISSLSHLHVYQSQNGEWVFYPTMEDKGEVVDFVVFHNIIYVVTLKANIGVLSLNSANIKFLKLNSTPNEIYYYNLRLVICDEQLLVVNRQIKNVFKIEGSTMNYVKLKTLGDIALFYDADNFRANCHVLSNPNLWGYESNSVYIINQFSTRCSVYSWADKKLQKCITVSTLDGTSSRMFDWCIRCLRYEIDYSLVE*