AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0171310

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0171310 MtrunA17_Chr1g0189391 78.670 361 31 4 71 412 1 334 0.0 527
Msa0171310 MtrunA17_Chr6g0485431 47.202 411 159 9 10 412 1 361 1.94e-110 328
Msa0171310 MtrunA17_Chr7g0218081 51.852 81 38 1 138 218 387 466 4.03e-22 98.6
Msa0171310 MtrunA17_Chr7g0218081 43.023 86 43 3 161 246 228 307 7.77e-12 67.0
Msa0171310 MtrunA17_Chr7g0243891 34.807 181 106 5 100 273 306 481 9.00e-22 97.4
Msa0171310 MtrunA17_Chr7g0243891 50.000 58 27 2 161 218 216 271 8.22e-11 63.9
Msa0171310 MtrunA17_Chr7g0249311 49.474 95 44 3 125 218 357 448 1.04e-21 97.4
Msa0171310 MtrunA17_Chr3g0079691 35.882 170 94 5 74 236 406 567 2.32e-21 96.7
Msa0171310 MtrunA17_Chr4g0042831 46.875 96 49 2 124 218 240 334 1.26e-20 92.8
Msa0171310 MtrunA17_Chr5g0418191 47.423 97 50 1 143 239 302 397 1.32e-20 93.2
Msa0171310 MtrunA17_Chr4g0004911 45.714 105 55 2 114 218 290 392 1.39e-20 93.6
Msa0171310 MtrunA17_Chr5g0445311 33.962 159 100 3 61 218 266 420 2.55e-20 93.2
Msa0171310 MtrunA17_Chr7g0249301 54.930 71 31 1 148 218 279 348 4.22e-20 91.3
Msa0171310 MtrunA17_Chr1g0150801 50.667 75 36 1 144 218 85 158 4.28e-20 86.7
Msa0171310 MtrunA17_Chr8g0383251 51.389 72 34 1 147 218 94 164 6.30e-20 87.0
Msa0171310 MtrunA17_Chr3g0129361 52.000 75 35 1 146 220 129 202 1.01e-19 87.4
Msa0171310 MtrunA17_Chr5g0432161 52.500 80 37 1 139 218 399 477 1.09e-19 91.3
Msa0171310 MtrunA17_Chr8g0383351 53.521 71 32 1 153 223 152 221 1.46e-19 87.0
Msa0171310 MtrunA17_Chr3g0101131 36.129 155 91 4 64 218 325 471 1.60e-19 90.9
Msa0171310 MtrunA17_Chr3g0106491 54.795 73 32 1 146 218 350 421 3.03e-19 89.7
Msa0171310 MtrunA17_Chr3g0106491 43.333 90 42 4 161 249 198 279 3.44e-11 65.1
Msa0171310 MtrunA17_Chr7g0270191 41.905 105 48 3 146 249 130 222 4.39e-19 87.0
Msa0171310 MtrunA17_Chr3g0129321 48.000 75 38 1 144 218 101 174 8.48e-18 81.6
Msa0171310 MtrunA17_Chr4g0051881 43.810 105 51 4 148 252 165 261 8.86e-18 84.0
Msa0171310 MtrunA17_Chr8g0335811 48.718 78 39 1 146 223 139 215 9.09e-18 82.0
Msa0171310 MtrunA17_Chr7g0263531 51.351 74 35 1 145 218 345 417 1.26e-17 84.3
Msa0171310 MtrunA17_Chr4g0068541 48.684 76 37 2 148 222 195 269 3.46e-17 83.2
Msa0171310 MtrunA17_Chr5g0447701 45.333 75 40 1 146 220 237 310 4.79e-17 82.4
Msa0171310 MtrunA17_Chr1g0194481 49.315 73 36 1 146 218 143 214 4.99e-17 81.6
Msa0171310 MtrunA17_Chr7g0224801 52.857 70 28 2 151 218 83 149 1.80e-16 76.6
Msa0171310 MtrunA17_Chr7g0248721 47.887 71 35 1 148 218 128 196 3.85e-16 77.0
Msa0171310 MtrunA17_Chr1g0188941 50.725 69 33 1 150 218 40 107 3.96e-16 74.3
Msa0171310 MtrunA17_Chr7g0238921 46.154 78 40 2 142 218 104 180 2.54e-15 76.3
Msa0171310 MtrunA17_Chr8g0386241 44.595 74 40 1 145 218 87 159 4.25e-15 73.2
Msa0171310 MtrunA17_Chr5g0418781 45.946 74 39 1 148 221 111 183 4.35e-15 75.1
Msa0171310 MtrunA17_Chr5g0443131 46.479 71 37 1 148 218 144 213 5.54e-15 75.5
Msa0171310 MtrunA17_Chr4g0074001 38.462 104 62 2 124 226 321 423 5.04e-14 73.6
Msa0171310 MtrunA17_Chr7g0270831 38.235 102 57 3 122 218 173 273 7.83e-14 72.8
Msa0171310 MtrunA17_Chr2g0294231 32.192 146 85 3 86 218 115 259 9.65e-14 72.4
Msa0171310 MtrunA17_Chr2g0322391 40.449 89 43 3 140 219 320 407 1.15e-13 72.8
Msa0171310 MtrunA17_Chr8g0348311 39.216 102 45 4 118 218 127 212 3.65e-13 70.1
Msa0171310 MtrunA17_Chr3g0139021 40.594 101 47 4 119 218 283 371 5.24e-13 70.1
Msa0171310 MtrunA17_Chr4g0075201 43.056 72 39 2 148 218 254 324 1.07e-12 69.7
Msa0171310 MtrunA17_Chr3g0099641 42.466 73 40 2 148 219 238 309 1.32e-12 69.3
Msa0171310 MtrunA17_Chr2g0288871 43.056 72 39 2 148 218 292 362 1.44e-12 69.3
Msa0171310 MtrunA17_Chr1g0147881 38.532 109 47 4 115 218 226 319 1.60e-12 68.2
Msa0171310 MtrunA17_Chr2g0302391 45.833 72 37 2 148 218 198 268 2.46e-12 68.2
Msa0171310 MtrunA17_Chr2g0284321 54.237 59 25 2 162 219 36 93 3.51e-12 65.5
Msa0171310 MtrunA17_Chr4g0025061 44.595 74 35 3 148 218 347 417 4.78e-12 67.8
Msa0171310 MtrunA17_Chr7g0228941 49.254 67 32 2 158 223 49 114 5.25e-12 62.8
Msa0171310 MtrunA17_Chr8g0387501 42.424 99 45 4 160 257 128 215 7.61e-12 65.5
Msa0171310 MtrunA17_Chr2g0333851 35.135 111 58 5 148 255 154 253 9.95e-12 65.5
Msa0171310 MtrunA17_Chr1g0150281 40.000 75 41 3 148 219 125 198 1.49e-11 64.7
Msa0171310 MtrunA17_Chr7g0270051 51.724 58 26 2 162 218 54 110 1.58e-11 64.3
Msa0171310 MtrunA17_Chr4g0071521 46.970 66 33 2 158 222 242 306 2.26e-11 64.7
Msa0171310 MtrunA17_Chr5g0401821 46.753 77 35 3 148 219 182 257 2.89e-11 63.5
Msa0171310 MtrunA17_Chr1g0184691 49.231 65 31 2 158 221 290 353 3.46e-11 64.3
Msa0171310 MtrunA17_Chr4g0060271 41.096 73 41 2 148 219 242 313 3.94e-11 65.1
Msa0171310 MtrunA17_Chr1g0202461 38.532 109 57 5 148 255 249 348 5.47e-11 63.9
Msa0171310 MtrunA17_Chr7g0216891 43.056 72 39 2 148 218 206 276 6.32e-11 64.3
Msa0171310 MtrunA17_Chr4g0053281 49.206 63 30 2 158 219 263 324 6.66e-11 63.2
Msa0171310 MtrunA17_Chr7g0248691 49.206 63 30 2 157 218 150 211 7.53e-11 63.2
Msa0171310 MtrunA17_Chr4g0025051 41.558 77 43 2 143 218 317 392 7.96e-11 63.9
Msa0171310 MtrunA17_Chr2g0293481 45.455 66 34 2 154 218 260 324 8.01e-11 63.5
Msa0171310 MtrunA17_Chr2g0319961 52.542 59 26 2 162 219 81 138 9.65e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0171310 AT5G64810.1 38.835 103 62 1 116 218 65 166 1.47e-21 92.0
Msa0171310 AT1G64000.1 55.556 72 31 1 147 218 100 170 2.58e-21 91.7
Msa0171310 AT2G38470.1 36.364 165 98 4 57 218 258 418 5.47e-21 95.5
Msa0171310 AT5G41570.1 54.930 71 31 1 148 218 85 154 1.68e-20 88.6
Msa0171310 AT4G26640.7 42.609 115 54 2 115 218 284 397 1.75e-20 94.0
Msa0171310 AT4G26640.2 42.609 115 54 2 115 218 324 437 2.51e-20 93.6
Msa0171310 AT4G26640.1 42.609 115 54 2 115 218 252 365 2.79e-20 93.2
Msa0171310 AT5G07100.1 52.381 84 32 2 146 222 219 301 3.02e-20 90.9
Msa0171310 AT3G01080.2 53.947 76 34 1 143 218 326 400 3.47e-20 92.8
Msa0171310 AT1G13960.1 53.947 76 34 1 143 218 391 465 3.75e-20 92.8
Msa0171310 AT5G07100.2 52.381 84 32 2 146 222 126 208 3.92e-20 88.6
Msa0171310 AT5G07100.4 52.381 84 32 2 146 222 126 208 3.92e-20 88.6
Msa0171310 AT5G07100.5 52.381 84 32 2 146 222 126 208 3.92e-20 88.6
Msa0171310 AT5G07100.3 52.381 84 32 2 146 222 126 208 3.92e-20 88.6
Msa0171310 AT4G26640.5 54.795 73 32 1 146 218 190 261 4.03e-20 91.7
Msa0171310 AT4G26640.6 54.795 73 32 1 146 218 190 261 4.03e-20 91.7
Msa0171310 AT4G26640.4 54.795 73 32 1 146 218 190 261 4.03e-20 91.7
Msa0171310 AT4G26640.3 54.795 73 32 1 146 218 190 261 4.03e-20 91.7
Msa0171310 AT2G03340.1 53.947 76 34 1 143 218 397 471 4.13e-20 92.8
Msa0171310 AT3G01080.1 53.947 76 34 1 143 218 288 362 7.14e-20 91.7
Msa0171310 AT1G13960.2 53.947 76 34 1 143 218 238 312 1.16e-19 90.1
Msa0171310 AT4G26440.1 44.860 107 56 2 112 218 325 428 1.41e-19 91.3
Msa0171310 AT5G56270.1 54.795 73 32 1 146 218 472 543 2.84e-19 90.5
Msa0171310 AT1G55600.1 52.632 76 35 1 143 218 289 363 9.91e-19 88.6
Msa0171310 AT2G04880.2 37.190 121 75 1 98 218 220 339 3.59e-18 86.7
Msa0171310 AT2G04880.1 37.190 121 75 1 98 218 244 363 4.77e-18 86.3
Msa0171310 AT2G44745.1 39.623 106 52 2 135 229 108 212 5.34e-18 82.8
Msa0171310 AT2G46130.1 52.113 71 33 1 148 218 17 86 5.73e-18 79.7
Msa0171310 AT2G30250.1 52.055 73 34 1 146 218 313 384 6.85e-18 85.5
Msa0171310 AT5G49520.1 52.055 73 34 1 146 218 206 277 7.14e-18 85.5
Msa0171310 AT2G47260.1 49.315 73 36 1 146 218 159 230 1.02e-17 84.3
Msa0171310 AT5G26170.1 43.243 74 41 1 148 221 100 172 2.16e-17 79.7
Msa0171310 AT1G29860.1 49.315 73 36 1 146 218 121 192 2.38e-17 82.4
Msa0171310 AT4G39410.1 49.333 75 37 1 146 220 208 281 2.92e-17 82.4
Msa0171310 AT4G18170.1 47.945 73 37 1 146 218 157 228 2.00e-16 80.1
Msa0171310 AT1G69310.4 45.946 74 39 1 148 221 134 206 5.10e-16 78.6
Msa0171310 AT1G69310.2 45.946 74 39 1 148 221 134 206 5.10e-16 78.6
Msa0171310 AT1G69310.1 45.946 74 39 1 148 221 134 206 5.10e-16 78.6
Msa0171310 AT3G62340.1 52.113 71 33 1 148 218 105 174 6.47e-16 77.8
Msa0171310 AT5G13080.1 48.649 74 33 2 147 218 53 123 8.44e-16 74.7
Msa0171310 AT5G46350.1 45.070 71 38 1 148 218 170 239 2.23e-15 77.0
Msa0171310 AT3G01970.1 48.052 77 37 2 148 224 52 125 2.81e-15 73.2
Msa0171310 AT2G21900.1 51.562 64 29 2 156 218 103 165 3.08e-15 74.3
Msa0171310 AT2G46130.2 47.887 71 32 1 148 218 17 82 3.34e-15 71.6
Msa0171310 AT4G31550.2 37.903 124 58 5 116 238 211 316 8.83e-15 75.5
Msa0171310 AT1G69310.3 41.892 74 38 1 148 221 134 202 8.37e-14 72.0
Msa0171310 AT4G30935.1 39.252 107 55 3 124 226 295 395 1.45e-13 72.4
Msa0171310 AT2G37260.1 49.206 63 31 1 156 218 344 405 2.36e-13 71.6
Msa0171310 AT2G37260.3 49.206 63 31 1 156 218 264 325 2.79e-13 71.2
Msa0171310 AT2G37260.2 49.206 63 31 1 156 218 262 323 2.85e-13 70.9
Msa0171310 AT4G31550.1 37.600 125 58 6 116 238 211 317 2.97e-13 70.9
Msa0171310 AT4G01250.1 41.000 100 43 4 120 218 101 185 5.85e-13 69.7
Msa0171310 AT5G52830.1 38.532 109 59 3 146 253 150 251 7.71e-13 69.7
Msa0171310 AT4G23550.1 43.243 74 40 2 146 218 125 197 7.86e-13 69.3
Msa0171310 AT4G04450.2 44.444 72 38 2 148 218 185 255 1.70e-12 69.3
Msa0171310 AT2G25000.2 34.862 109 55 5 127 220 56 163 2.80e-12 66.6
Msa0171310 AT2G25000.4 34.862 109 55 5 127 220 56 163 2.80e-12 66.6
Msa0171310 AT1G29280.1 43.011 93 48 3 127 218 43 131 2.93e-12 67.0
Msa0171310 AT2G46400.1 41.667 84 45 2 148 228 91 173 3.83e-12 67.0
Msa0171310 AT4G24240.1 44.444 81 39 3 148 223 264 343 4.40e-12 67.4
Msa0171310 AT2G25000.3 34.862 109 55 5 127 220 78 185 4.48e-12 66.2
Msa0171310 AT4G04450.1 44.444 72 38 2 148 218 279 349 4.54e-12 68.2
Msa0171310 AT2G23320.1 43.750 80 39 3 145 219 220 298 5.29e-12 67.0
Msa0171310 AT4G22070.4 43.056 72 39 2 148 218 284 354 5.65e-12 67.8
Msa0171310 AT4G22070.3 43.056 72 39 2 148 218 284 354 5.65e-12 67.8
Msa0171310 AT4G22070.2 43.056 72 39 2 148 218 284 354 5.65e-12 67.8
Msa0171310 AT4G22070.1 43.056 72 39 2 148 218 284 354 5.65e-12 67.8
Msa0171310 AT5G43290.1 43.056 72 39 2 148 218 100 170 6.73e-12 66.2
Msa0171310 AT2G25000.1 34.862 109 55 5 127 220 99 206 8.38e-12 65.9
Msa0171310 AT4G01720.1 44.444 72 38 2 148 218 226 296 1.09e-11 67.0
Msa0171310 AT5G15130.2 43.056 72 39 2 148 218 172 242 1.16e-11 66.6
Msa0171310 AT1G62300.1 43.056 72 39 2 148 218 299 369 1.35e-11 66.6
Msa0171310 AT5G15130.1 43.056 72 39 2 148 218 214 284 1.42e-11 66.6
Msa0171310 AT2G24570.1 40.000 105 49 5 116 218 208 300 2.31e-11 65.1
Msa0171310 AT2G34830.1 47.692 65 32 2 155 218 209 272 5.04e-11 64.7
Msa0171310 AT2G34830.2 47.692 65 32 2 155 218 218 281 5.40e-11 64.7

Find 113 sgRNAs with CRISPR-Local

Find 318 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAAGTGATGAAGACATTT+TGG 0.222211 1_4:+56014421 Msa0171310:CDS
ATCAGTTGTACTATCTTCTT+TGG 0.230642 1_4:-56014064 None:intergenic
TGCTCCAGTCTTTCCTTTCA+TGG 0.232695 1_4:+56015843 Msa0171310:CDS
AATAATTACCTACGATTGTT+AGG 0.259815 1_4:-56014494 None:intergenic
GGAATTGGCCCGTTGATATT+TGG 0.273233 1_4:-56015637 None:intergenic
TATGTAACAAGTACATATTT+TGG 0.310515 1_4:-56015289 None:intergenic
CCAACTTGAGTTGTGGTAAT+AGG 0.316992 1_4:-56015700 None:intergenic
TTTCTTCAGGTTGGTGGAAA+TGG 0.328210 1_4:+56015811 Msa0171310:CDS
AAAGGCCGCGTCATGGTATT+AGG 0.337576 1_4:-56015508 None:intergenic
TTACCCACCATGTTTAATAA+AGG 0.347175 1_4:-56015526 None:intergenic
GTCCTTGATCAGGTGGTGTT+GGG 0.367401 1_4:-56013921 None:intergenic
AACTCCTATTGATACAAATC+AGG 0.367991 1_4:+56015609 Msa0171310:CDS
ATACCTTATGGTATGAATTA+CGG 0.368502 1_4:+56015481 Msa0171310:CDS
CAATATGGCTATATACCTTA+TGG 0.368992 1_4:+56015469 Msa0171310:CDS
CATAAGGTATATAGCCATAT+TGG 0.374743 1_4:-56015468 None:intergenic
GGGTACTTCCCCAGCTGATC+AGG 0.376476 1_4:+56015864 Msa0171310:CDS
TCGAGAAATATTAATGCATC+TGG 0.384031 1_4:+56015364 Msa0171310:CDS
GGAAATACAGGAAGCGGAAT+TGG 0.386630 1_4:-56015652 None:intergenic
CCTAAATATCATTCGGTGTC+AGG 0.391994 1_4:-56015901 None:intergenic
TGCAATTCAGGAAGAGAAAG+AGG 0.396225 1_4:+56014330 Msa0171310:intron
GCTCCAGTCTTTCCTTTCAT+GGG 0.399255 1_4:+56015844 Msa0171310:CDS
CCATTTCACATTGTACCATA+TGG 0.401565 1_4:+56015781 Msa0171310:CDS
TATGTACTTGTTACATATGA+TGG 0.402941 1_4:+56015295 Msa0171310:CDS
TGTTCACTTATGGGTCTGTA+AGG 0.409849 1_4:-56015562 None:intergenic
TCTGCACCATGTCCTTGATC+AGG 0.410286 1_4:-56013931 None:intergenic
AAACGATTATAAAACCTTTG+TGG 0.423869 1_4:-56014210 None:intergenic
CAAGTTGGACATAATATCAA+TGG 0.426656 1_4:+56015715 Msa0171310:CDS
CCAACCTGATTTGTATCAAT+AGG 0.427285 1_4:-56015613 None:intergenic
GGTGGTAATGGTGGTGATAT+CGG 0.428089 1_4:-56015673 None:intergenic
GGTGGTAATGGTGGTGATAT+CGG 0.428089 1_4:-56015760 None:intergenic
ATTGCTTCAGAAAAGTTCAT+TGG 0.428330 1_4:-56014036 None:intergenic
TCAGTAGCTTCCTGATCAGC+TGG 0.438453 1_4:-56015874 None:intergenic
GGTGGTGATATCGGAAATAC+AGG 0.454748 1_4:-56015664 None:intergenic
GGTGGTGATATCGGAAATAC+AGG 0.454748 1_4:-56015751 None:intergenic
CAACCGTAATTCATACCATA+AGG 0.481218 1_4:-56015484 None:intergenic
TGTCCTTGATCAGGTGGTGT+TGG 0.482577 1_4:-56013922 None:intergenic
ATATAGCCATATTGGTGAAA+AGG 0.483202 1_4:-56015460 None:intergenic
GTAATAGGAAATGGTGGTAA+TGG 0.484085 1_4:-56015685 None:intergenic
ACCACCATCTGCATTGCCTA+TGG 0.489573 1_4:+56015423 Msa0171310:CDS
TGAAGAAAATCCCCACCATA+TGG 0.492962 1_4:-56015796 None:intergenic
TTAATATTTCTCGAGTATGT+TGG 0.504174 1_4:-56015355 None:intergenic
GTTGAACTTCAGTTTCAGCT+TGG 0.505017 1_4:-56013876 None:intergenic
CCTATTACCACAACTCAAGT+TGG 0.508963 1_4:+56015700 Msa0171310:CDS
TATGTAGCCTAAATATCATT+CGG 0.509419 1_4:-56015908 None:intergenic
AGGTTGGACCAAATATCAAC+GGG 0.512849 1_4:+56015629 Msa0171310:CDS
GATGATGAAGCAACTGCCAT+TGG 0.519242 1_4:+56014138 Msa0171310:CDS
CATCTCCTCTTGTTGCCACA+TGG 0.526145 1_4:-56013846 None:intergenic
TTCACATTGTACCATATGGT+GGG 0.526608 1_4:+56015785 Msa0171310:CDS
CCATATGGTACAATGTGAAA+TGG 0.527122 1_4:-56015781 None:intergenic
TGAGTTGTGGTAATAGGAAA+TGG 0.532382 1_4:-56015694 None:intergenic
ATGCTGAAATTCTAAGGGCT+AGG 0.533181 1_4:+56014001 Msa0171310:CDS
AAGTTGGACATAATATCAAT+GGG 0.540013 1_4:+56015716 Msa0171310:CDS
TCCCCAACACCACCTGATCA+AGG 0.540123 1_4:+56013919 Msa0171310:CDS
GTACCCATGAAAGGAAAGAC+TGG 0.542215 1_4:-56015847 None:intergenic
TCGACATCTGTCATGGGGCA+AGG 0.545512 1_4:-56014377 None:intergenic
GGAGGGAGAGGGTGAACCAT+AGG 0.549300 1_4:-56015439 None:intergenic
GATGAAGCAACTGCCATTGG+AGG 0.552119 1_4:+56014141 Msa0171310:CDS
GGTTATTGTTGGCGCAAGTA+CGG 0.553147 1_4:+56014449 Msa0171310:CDS
AAATTCTAAGGGCTAGGAAA+GGG 0.557406 1_4:+56014007 Msa0171310:CDS
CAGTAGCTTCCTGATCAGCT+GGG 0.557669 1_4:-56015873 None:intergenic
TCACATTGTACCATATGGTG+GGG 0.559672 1_4:+56015786 Msa0171310:CDS
ACAATGTGAAATGGTGGTAA+TGG 0.559807 1_4:-56015772 None:intergenic
GATATCGGAAATACAGGAAG+CGG 0.565608 1_4:-56015658 None:intergenic
ATAGGAAATGGTGGTAATGG+TGG 0.566411 1_4:-56015682 None:intergenic
TATTGGTGAAAAGGAGGGAG+AGG 0.567707 1_4:-56015451 None:intergenic
TTTGTCAATGACAGGTCCGT+GGG 0.568911 1_4:-56015326 None:intergenic
GAAATTCTAAGGGCTAGGAA+AGG 0.571481 1_4:+56014006 Msa0171310:CDS
TTTCACATTGTACCATATGG+TGG 0.572755 1_4:+56015784 Msa0171310:CDS
TATGATGATGGTGGTGCTAG+TGG 0.572944 1_4:+56014180 Msa0171310:CDS
CCTATTGATACAAATCAGGT+TGG 0.576330 1_4:+56015613 Msa0171310:CDS
GCAATTCAGGAAGAGAAAGA+GGG 0.576835 1_4:+56014331 Msa0171310:intron
AAGCAACATCATTCCTCCAA+TGG 0.581591 1_4:-56014154 None:intergenic
TAGCCATATTGGTGAAAAGG+AGG 0.582097 1_4:-56015457 None:intergenic
TCCTTGATCAGGTGGTGTTG+GGG 0.585344 1_4:-56013920 None:intergenic
GTTTAATAAAGGCCGCGTCA+TGG 0.585854 1_4:-56015515 None:intergenic
GCAGATGGTGGTGATGGTGG+TGG 0.586837 1_4:-56015412 None:intergenic
GCAGTTGCTTCATCATCCAC+AGG 0.588792 1_4:-56014132 None:intergenic
CTTATGGTATGAATTACGGT+TGG 0.590836 1_4:+56015485 Msa0171310:CDS
TGATGGTGGTGGCAGCATGT+TGG 0.595809 1_4:-56015401 None:intergenic
AATGCAGATGGTGGTGATGG+TGG 0.599942 1_4:-56015415 None:intergenic
CGGCCTTTATTAAACATGGT+GGG 0.600743 1_4:+56015523 Msa0171310:CDS
CAGGTTGGACCAAATATCAA+CGG 0.601960 1_4:+56015628 Msa0171310:CDS
TACGGATGCAAAGAGATCAA+AGG 0.609552 1_4:+56014467 Msa0171310:CDS
ATGTTGCTTCAATATGATGA+TGG 0.610984 1_4:+56014168 Msa0171310:CDS
ATGTGAAATGGTGGTAATGG+TGG 0.612583 1_4:-56015769 None:intergenic
GCTGGGGAAGTACCCATGAA+AGG 0.612956 1_4:-56015856 None:intergenic
GCACCATGTCCTTGATCAGG+TGG 0.613784 1_4:-56013928 None:intergenic
GACGCGGCCTTTATTAAACA+TGG 0.614916 1_4:+56015519 Msa0171310:CDS
TCTGAAACTTGATCATCAGT+TGG 0.615285 1_4:-56014105 None:intergenic
AAGGAAATCCTAACAATCGT+AGG 0.617006 1_4:+56014486 Msa0171310:CDS
TTTGCTCGACATCTGTCATG+GGG 0.618862 1_4:-56014382 None:intergenic
GGCAATGCAGATGGTGGTGA+TGG 0.620508 1_4:-56015418 None:intergenic
GCGGCCTTTATTAAACATGG+TGG 0.630164 1_4:+56015522 Msa0171310:CDS
TGAACCATAGGCAATGCAGA+TGG 0.643972 1_4:-56015427 None:intergenic
ATTGGTGAAAAGGAGGGAGA+GGG 0.644988 1_4:-56015450 None:intergenic
GATTGCCATGTGGCAACAAG+AGG 0.647237 1_4:+56013841 Msa0171310:CDS
ATTATGTCCAACTTGAGTTG+TGG 0.653953 1_4:-56015707 None:intergenic
AGCCATATTGGTGAAAAGGA+GGG 0.654609 1_4:-56015456 None:intergenic
TATGATGGAAAACATACCCA+CGG 0.656713 1_4:+56015310 Msa0171310:CDS
GGGTCTGTAAGGTTGCATCA+TGG 0.657757 1_4:-56015551 None:intergenic
GCAAAATGATGCAAATGTAG+TGG 0.669148 1_4:+56013972 Msa0171310:CDS
AGTAGCTTCCTGATCAGCTG+GGG 0.677547 1_4:-56015872 None:intergenic
CTAGTGGATCTTGTCCACAA+AGG 0.681150 1_4:+56014196 Msa0171310:CDS
ACATCTGTCATGGGGCAAGG+TGG 0.684322 1_4:-56014374 None:intergenic
GGGGATGAGAAATTCAACAC+TGG 0.684419 1_4:-56013901 None:intergenic
GTTGTGGTAATAGGAAATGG+TGG 0.688257 1_4:-56015691 None:intergenic
TTCTGCTGATGATTGCCATG+TGG 0.692089 1_4:+56013831 Msa0171310:CDS
TATGGTACAATGTGAAATGG+TGG 0.694153 1_4:-56015778 None:intergenic
ACCATAGGCAATGCAGATGG+TGG 0.695874 1_4:-56015424 None:intergenic
TTGCTTCAATATGATGATGG+TGG 0.702055 1_4:+56014171 Msa0171310:CDS
ACACCACCTGATCAAGGACA+TGG 0.702867 1_4:+56013925 Msa0171310:CDS
TCAAGTTTCAGATACACCTG+TGG 0.733378 1_4:+56014116 Msa0171310:CDS
GTTGGCCTAATACCATGACG+CGG 0.807585 1_4:+56015503 Msa0171310:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTAAAAATATCAAAAACA+AGG - chr1_4:56014799-56014818 None:intergenic 15.0%
!!! CTTTATCATTTTATTTTAAG+CGG + chr1_4:56015026-56015045 Msa0171310:intron 15.0%
!! TACTAATACATTTATGTAAA+TGG - chr1_4:56015132-56015151 None:intergenic 15.0%
!! TCACACAATATTAAATAATT+AGG - chr1_4:56015195-56015214 None:intergenic 15.0%
!! TTGTAAAAATATCAAAAACA+AGG - chr1_4:56014799-56014818 None:intergenic 15.0%
!!! CTTTATCATTTTATTTTAAG+CGG + chr1_4:56015026-56015045 Msa0171310:intron 15.0%
!! TACTAATACATTTATGTAAA+TGG - chr1_4:56015132-56015151 None:intergenic 15.0%
!! TCACACAATATTAAATAATT+AGG - chr1_4:56015195-56015214 None:intergenic 15.0%
!! GTAATTGAATCAAATTCATT+AGG + chr1_4:56013807-56013826 Msa0171310:intron 20.0%
!! ACATTGTTGCTAATAAATAA+AGG + chr1_4:56014278-56014297 Msa0171310:intron 20.0%
!!! TTTTACGATAAATCACTTAT+CGG - chr1_4:56014929-56014948 None:intergenic 20.0%
!! CAAATTCAATATCAAATTCT+AGG - chr1_4:56014956-56014975 None:intergenic 20.0%
!! AGAATTTGATATTGAATTTG+TGG + chr1_4:56014956-56014975 Msa0171310:intron 20.0%
!! TTTAATATTGTGTGAATTGT+AGG + chr1_4:56015200-56015219 Msa0171310:intron 20.0%
!! TTAATATTGTGTGAATTGTA+GGG + chr1_4:56015201-56015220 Msa0171310:intron 20.0%
!!! TATGTAACAAGTACATATTT+TGG - chr1_4:56015292-56015311 None:intergenic 20.0%
!!! AGAAAATTTTGTTCACTTAT+GGG - chr1_4:56015574-56015593 None:intergenic 20.0%
!!! TAGAAAATTTTGTTCACTTA+TGG - chr1_4:56015575-56015594 None:intergenic 20.0%
!! GTAATTGAATCAAATTCATT+AGG + chr1_4:56013807-56013826 Msa0171310:intron 20.0%
!! ACATTGTTGCTAATAAATAA+AGG + chr1_4:56014278-56014297 Msa0171310:intron 20.0%
!!! TTTTACGATAAATCACTTAT+CGG - chr1_4:56014929-56014948 None:intergenic 20.0%
!! CAAATTCAATATCAAATTCT+AGG - chr1_4:56014956-56014975 None:intergenic 20.0%
!! AGAATTTGATATTGAATTTG+TGG + chr1_4:56014956-56014975 Msa0171310:intron 20.0%
!! TTTAATATTGTGTGAATTGT+AGG + chr1_4:56015200-56015219 Msa0171310:intron 20.0%
!! TTAATATTGTGTGAATTGTA+GGG + chr1_4:56015201-56015220 Msa0171310:intron 20.0%
!!! TATGTAACAAGTACATATTT+TGG - chr1_4:56015292-56015311 None:intergenic 20.0%
!!! AGAAAATTTTGTTCACTTAT+GGG - chr1_4:56015574-56015593 None:intergenic 20.0%
!!! TAGAAAATTTTGTTCACTTA+TGG - chr1_4:56015575-56015594 None:intergenic 20.0%
! TGAAAAATGCTGAAATTCTA+AGG + chr1_4:56013995-56014014 Msa0171310:CDS 25.0%
! GAAAAATGCTGAAATTCTAA+GGG + chr1_4:56013996-56014015 Msa0171310:CDS 25.0%
! AAACGATTATAAAACCTTTG+TGG - chr1_4:56014213-56014232 None:intergenic 25.0%
! AATAATTACCTACGATTGTT+AGG - chr1_4:56014497-56014516 None:intergenic 25.0%
! TGCATTAACACATATAAAAG+AGG - chr1_4:56014522-56014541 None:intergenic 25.0%
! AATGACATAATGATCATTGA+CGG + chr1_4:56014737-56014756 Msa0171310:intron 25.0%
! AATCTAACAATGATTGATGT+TGG - chr1_4:56014866-56014885 None:intergenic 25.0%
!!! TAGAGATGATTTTCAAGTAT+TGG + chr1_4:56014886-56014905 Msa0171310:intron 25.0%
!!! AGAGATGATTTTCAAGTATT+GGG + chr1_4:56014887-56014906 Msa0171310:intron 25.0%
! TAATATTGTGTGAATTGTAG+GGG + chr1_4:56015202-56015221 Msa0171310:intron 25.0%
! TTGTGACATTAAAAAACAAG+TGG + chr1_4:56015249-56015268 Msa0171310:CDS 25.0%
! ACATTAAAAAACAAGTGGAA+AGG + chr1_4:56015254-56015273 Msa0171310:CDS 25.0%
! CATTAAAAAACAAGTGGAAA+GGG + chr1_4:56015255-56015274 Msa0171310:CDS 25.0%
!! TATGTACTTGTTACATATGA+TGG + chr1_4:56015295-56015314 Msa0171310:CDS 25.0%
! TTAATATTTCTCGAGTATGT+TGG - chr1_4:56015358-56015377 None:intergenic 25.0%
! ATACCTTATGGTATGAATTA+CGG + chr1_4:56015481-56015500 Msa0171310:CDS 25.0%
! AAGTTGGACATAATATCAAT+GGG + chr1_4:56015716-56015735 Msa0171310:CDS 25.0%
! TGAAAAATGCTGAAATTCTA+AGG + chr1_4:56013995-56014014 Msa0171310:CDS 25.0%
! GAAAAATGCTGAAATTCTAA+GGG + chr1_4:56013996-56014015 Msa0171310:CDS 25.0%
! AAACGATTATAAAACCTTTG+TGG - chr1_4:56014213-56014232 None:intergenic 25.0%
! AATAATTACCTACGATTGTT+AGG - chr1_4:56014497-56014516 None:intergenic 25.0%
! TGCATTAACACATATAAAAG+AGG - chr1_4:56014522-56014541 None:intergenic 25.0%
! AATGACATAATGATCATTGA+CGG + chr1_4:56014737-56014756 Msa0171310:intron 25.0%
! AATCTAACAATGATTGATGT+TGG - chr1_4:56014866-56014885 None:intergenic 25.0%
!!! TAGAGATGATTTTCAAGTAT+TGG + chr1_4:56014886-56014905 Msa0171310:intron 25.0%
!!! AGAGATGATTTTCAAGTATT+GGG + chr1_4:56014887-56014906 Msa0171310:intron 25.0%
! TAATATTGTGTGAATTGTAG+GGG + chr1_4:56015202-56015221 Msa0171310:intron 25.0%
! TTGTGACATTAAAAAACAAG+TGG + chr1_4:56015249-56015268 Msa0171310:CDS 25.0%
! ACATTAAAAAACAAGTGGAA+AGG + chr1_4:56015254-56015273 Msa0171310:CDS 25.0%
! CATTAAAAAACAAGTGGAAA+GGG + chr1_4:56015255-56015274 Msa0171310:CDS 25.0%
!! TATGTACTTGTTACATATGA+TGG + chr1_4:56015295-56015314 Msa0171310:CDS 25.0%
! TTAATATTTCTCGAGTATGT+TGG - chr1_4:56015358-56015377 None:intergenic 25.0%
! ATACCTTATGGTATGAATTA+CGG + chr1_4:56015481-56015500 Msa0171310:CDS 25.0%
! AAGTTGGACATAATATCAAT+GGG + chr1_4:56015716-56015735 Msa0171310:CDS 25.0%
ATTGCTTCAGAAAAGTTCAT+TGG - chr1_4:56014039-56014058 None:intergenic 30.0%
ATCAGTTGTACTATCTTCTT+TGG - chr1_4:56014067-56014086 None:intergenic 30.0%
! ATGTTGCTTCAATATGATGA+TGG + chr1_4:56014168-56014187 Msa0171310:CDS 30.0%
!! GCTCAATTATTTTGCAATTC+AGG + chr1_4:56014318-56014337 Msa0171310:intron 30.0%
! TGAAAGTGATGAAGACATTT+TGG + chr1_4:56014421-56014440 Msa0171310:CDS 30.0%
!! TTTTGGATGATGGTTATTGT+TGG + chr1_4:56014438-56014457 Msa0171310:CDS 30.0%
GTCAATGATCATTATGTCAT+TGG - chr1_4:56014738-56014757 None:intergenic 30.0%
AATATTGTGTGAATTGTAGG+GGG + chr1_4:56015203-56015222 Msa0171310:intron 30.0%
TCGAGAAATATTAATGCATC+TGG + chr1_4:56015364-56015383 Msa0171310:CDS 30.0%
ATATAGCCATATTGGTGAAA+AGG - chr1_4:56015463-56015482 None:intergenic 30.0%
CATAAGGTATATAGCCATAT+TGG - chr1_4:56015471-56015490 None:intergenic 30.0%
CAATATGGCTATATACCTTA+TGG + chr1_4:56015469-56015488 Msa0171310:CDS 30.0%
TTACCCACCATGTTTAATAA+AGG - chr1_4:56015529-56015548 None:intergenic 30.0%
AACTCCTATTGATACAAATC+AGG + chr1_4:56015609-56015628 Msa0171310:CDS 30.0%
! CAAGTTGGACATAATATCAA+TGG + chr1_4:56015715-56015734 Msa0171310:CDS 30.0%
ATTGCTTCAGAAAAGTTCAT+TGG - chr1_4:56014039-56014058 None:intergenic 30.0%
ATCAGTTGTACTATCTTCTT+TGG - chr1_4:56014067-56014086 None:intergenic 30.0%
! ATGTTGCTTCAATATGATGA+TGG + chr1_4:56014168-56014187 Msa0171310:CDS 30.0%
!! GCTCAATTATTTTGCAATTC+AGG + chr1_4:56014318-56014337 Msa0171310:intron 30.0%
! TGAAAGTGATGAAGACATTT+TGG + chr1_4:56014421-56014440 Msa0171310:CDS 30.0%
!! TTTTGGATGATGGTTATTGT+TGG + chr1_4:56014438-56014457 Msa0171310:CDS 30.0%
GTCAATGATCATTATGTCAT+TGG - chr1_4:56014738-56014757 None:intergenic 30.0%
AATATTGTGTGAATTGTAGG+GGG + chr1_4:56015203-56015222 Msa0171310:intron 30.0%
TCGAGAAATATTAATGCATC+TGG + chr1_4:56015364-56015383 Msa0171310:CDS 30.0%
ATATAGCCATATTGGTGAAA+AGG - chr1_4:56015463-56015482 None:intergenic 30.0%
CATAAGGTATATAGCCATAT+TGG - chr1_4:56015471-56015490 None:intergenic 30.0%
CAATATGGCTATATACCTTA+TGG + chr1_4:56015469-56015488 Msa0171310:CDS 30.0%
TTACCCACCATGTTTAATAA+AGG - chr1_4:56015529-56015548 None:intergenic 30.0%
AACTCCTATTGATACAAATC+AGG + chr1_4:56015609-56015628 Msa0171310:CDS 30.0%
! CAAGTTGGACATAATATCAA+TGG + chr1_4:56015715-56015734 Msa0171310:CDS 30.0%
! CATTTGCATCATTTTGCACA+TGG - chr1_4:56013969-56013988 None:intergenic 35.0%
GCAAAATGATGCAAATGTAG+TGG + chr1_4:56013972-56013991 Msa0171310:CDS 35.0%
! AAATTCTAAGGGCTAGGAAA+GGG + chr1_4:56014007-56014026 Msa0171310:CDS 35.0%
! TCTGAAACTTGATCATCAGT+TGG - chr1_4:56014108-56014127 None:intergenic 35.0%
!! TTGCTTCAATATGATGATGG+TGG + chr1_4:56014171-56014190 Msa0171310:CDS 35.0%
! GATGAAGACATTTTGGATGA+TGG + chr1_4:56014428-56014447 Msa0171310:CDS 35.0%
AAGGAAATCCTAACAATCGT+AGG + chr1_4:56014486-56014505 Msa0171310:CDS 35.0%
TCTATATATACACCAAGGCT+AGG - chr1_4:56014586-56014605 None:intergenic 35.0%
CTCTCTCTATATATACACCA+AGG - chr1_4:56014591-56014610 None:intergenic 35.0%
! CTTGGTGTATATATAGAGAG+AGG + chr1_4:56014589-56014608 Msa0171310:intron 35.0%
!! TTATGTCATTGGCTTAGTTG+TGG - chr1_4:56014727-56014746 None:intergenic 35.0%
!! TGATTGATGTTGGTTCAACA+CGG - chr1_4:56014856-56014875 None:intergenic 35.0%
! TATGATGGAAAACATACCCA+CGG + chr1_4:56015310-56015329 Msa0171310:CDS 35.0%
!!! GGTCTTTTTTTGTCAATGAC+AGG - chr1_4:56015337-56015356 None:intergenic 35.0%
CAACCGTAATTCATACCATA+AGG - chr1_4:56015487-56015506 None:intergenic 35.0%
CTTATGGTATGAATTACGGT+TGG + chr1_4:56015485-56015504 Msa0171310:CDS 35.0%
! CCAACCTGATTTGTATCAAT+AGG - chr1_4:56015616-56015635 None:intergenic 35.0%
CCTATTGATACAAATCAGGT+TGG + chr1_4:56015613-56015632 Msa0171310:CDS 35.0%
GTAATAGGAAATGGTGGTAA+TGG - chr1_4:56015688-56015707 None:intergenic 35.0%
TGAGTTGTGGTAATAGGAAA+TGG - chr1_4:56015697-56015716 None:intergenic 35.0%
! ATTATGTCCAACTTGAGTTG+TGG - chr1_4:56015710-56015729 None:intergenic 35.0%
ACAATGTGAAATGGTGGTAA+TGG - chr1_4:56015775-56015794 None:intergenic 35.0%
TATGGTACAATGTGAAATGG+TGG - chr1_4:56015781-56015800 None:intergenic 35.0%
CCATATGGTACAATGTGAAA+TGG - chr1_4:56015784-56015803 None:intergenic 35.0%
CCATTTCACATTGTACCATA+TGG + chr1_4:56015781-56015800 Msa0171310:CDS 35.0%
TTTCACATTGTACCATATGG+TGG + chr1_4:56015784-56015803 Msa0171310:CDS 35.0%
TTCACATTGTACCATATGGT+GGG + chr1_4:56015785-56015804 Msa0171310:CDS 35.0%
! CATTTGCATCATTTTGCACA+TGG - chr1_4:56013969-56013988 None:intergenic 35.0%
GCAAAATGATGCAAATGTAG+TGG + chr1_4:56013972-56013991 Msa0171310:CDS 35.0%
! AAATTCTAAGGGCTAGGAAA+GGG + chr1_4:56014007-56014026 Msa0171310:CDS 35.0%
! TCTGAAACTTGATCATCAGT+TGG - chr1_4:56014108-56014127 None:intergenic 35.0%
!! TTGCTTCAATATGATGATGG+TGG + chr1_4:56014171-56014190 Msa0171310:CDS 35.0%
! GATGAAGACATTTTGGATGA+TGG + chr1_4:56014428-56014447 Msa0171310:CDS 35.0%
AAGGAAATCCTAACAATCGT+AGG + chr1_4:56014486-56014505 Msa0171310:CDS 35.0%
TCTATATATACACCAAGGCT+AGG - chr1_4:56014586-56014605 None:intergenic 35.0%
CTCTCTCTATATATACACCA+AGG - chr1_4:56014591-56014610 None:intergenic 35.0%
! CTTGGTGTATATATAGAGAG+AGG + chr1_4:56014589-56014608 Msa0171310:intron 35.0%
!! TTATGTCATTGGCTTAGTTG+TGG - chr1_4:56014727-56014746 None:intergenic 35.0%
!! TGATTGATGTTGGTTCAACA+CGG - chr1_4:56014856-56014875 None:intergenic 35.0%
! TATGATGGAAAACATACCCA+CGG + chr1_4:56015310-56015329 Msa0171310:CDS 35.0%
!!! GGTCTTTTTTTGTCAATGAC+AGG - chr1_4:56015337-56015356 None:intergenic 35.0%
CAACCGTAATTCATACCATA+AGG - chr1_4:56015487-56015506 None:intergenic 35.0%
CTTATGGTATGAATTACGGT+TGG + chr1_4:56015485-56015504 Msa0171310:CDS 35.0%
! CCAACCTGATTTGTATCAAT+AGG - chr1_4:56015616-56015635 None:intergenic 35.0%
CCTATTGATACAAATCAGGT+TGG + chr1_4:56015613-56015632 Msa0171310:CDS 35.0%
GTAATAGGAAATGGTGGTAA+TGG - chr1_4:56015688-56015707 None:intergenic 35.0%
TGAGTTGTGGTAATAGGAAA+TGG - chr1_4:56015697-56015716 None:intergenic 35.0%
! ATTATGTCCAACTTGAGTTG+TGG - chr1_4:56015710-56015729 None:intergenic 35.0%
ACAATGTGAAATGGTGGTAA+TGG - chr1_4:56015775-56015794 None:intergenic 35.0%
TATGGTACAATGTGAAATGG+TGG - chr1_4:56015781-56015800 None:intergenic 35.0%
CCATATGGTACAATGTGAAA+TGG - chr1_4:56015784-56015803 None:intergenic 35.0%
CCATTTCACATTGTACCATA+TGG + chr1_4:56015781-56015800 Msa0171310:CDS 35.0%
TTTCACATTGTACCATATGG+TGG + chr1_4:56015784-56015803 Msa0171310:CDS 35.0%
TTCACATTGTACCATATGGT+GGG + chr1_4:56015785-56015804 Msa0171310:CDS 35.0%
GTTGAACTTCAGTTTCAGCT+TGG - chr1_4:56013879-56013898 None:intergenic 40.0%
! ATGCTGAAATTCTAAGGGCT+AGG + chr1_4:56014001-56014020 Msa0171310:CDS 40.0%
! GAAATTCTAAGGGCTAGGAA+AGG + chr1_4:56014006-56014025 Msa0171310:CDS 40.0%
TCAAGTTTCAGATACACCTG+TGG + chr1_4:56014116-56014135 Msa0171310:CDS 40.0%
AAGCAACATCATTCCTCCAA+TGG - chr1_4:56014157-56014176 None:intergenic 40.0%
TGCAATTCAGGAAGAGAAAG+AGG + chr1_4:56014330-56014349 Msa0171310:intron 40.0%
GCAATTCAGGAAGAGAAAGA+GGG + chr1_4:56014331-56014350 Msa0171310:intron 40.0%
! TTTTGCTCGACATCTGTCAT+GGG - chr1_4:56014386-56014405 None:intergenic 40.0%
! TTTTTGCTCGACATCTGTCA+TGG - chr1_4:56014387-56014406 None:intergenic 40.0%
TACGGATGCAAAGAGATCAA+AGG + chr1_4:56014467-56014486 Msa0171310:CDS 40.0%
! TTTTCATTCACTCCTAGCCT+TGG + chr1_4:56014571-56014590 Msa0171310:intron 40.0%
GGTGTATATATAGAGAGAGG+AGG + chr1_4:56014592-56014611 Msa0171310:intron 40.0%
ACAGCAGTGTTCATCATCAT+TGG - chr1_4:56014669-56014688 None:intergenic 40.0%
TTGACGGTTTGACGAAAACA+TGG + chr1_4:56014753-56014772 Msa0171310:intron 40.0%
TGACGGTTTGACGAAAACAT+GGG + chr1_4:56014754-56014773 Msa0171310:intron 40.0%
AGCCATATTGGTGAAAAGGA+GGG - chr1_4:56015459-56015478 None:intergenic 40.0%
TAGCCATATTGGTGAAAAGG+AGG - chr1_4:56015460-56015479 None:intergenic 40.0%
CGGCCTTTATTAAACATGGT+GGG + chr1_4:56015523-56015542 Msa0171310:CDS 40.0%
TGTTCACTTATGGGTCTGTA+AGG - chr1_4:56015565-56015584 None:intergenic 40.0%
! CAGGTTGGACCAAATATCAA+CGG + chr1_4:56015628-56015647 Msa0171310:CDS 40.0%
AGGTTGGACCAAATATCAAC+GGG + chr1_4:56015629-56015648 Msa0171310:CDS 40.0%
GATATCGGAAATACAGGAAG+CGG - chr1_4:56015661-56015680 None:intergenic 40.0%
!! ATAGGAAATGGTGGTAATGG+TGG - chr1_4:56015685-56015704 None:intergenic 40.0%
GTTGTGGTAATAGGAAATGG+TGG - chr1_4:56015694-56015713 None:intergenic 40.0%
! CCAACTTGAGTTGTGGTAAT+AGG - chr1_4:56015703-56015722 None:intergenic 40.0%
CCTATTACCACAACTCAAGT+TGG + chr1_4:56015700-56015719 Msa0171310:CDS 40.0%
!! ATGTGAAATGGTGGTAATGG+TGG - chr1_4:56015772-56015791 None:intergenic 40.0%
TCACATTGTACCATATGGTG+GGG + chr1_4:56015786-56015805 Msa0171310:CDS 40.0%
TGAAGAAAATCCCCACCATA+TGG - chr1_4:56015799-56015818 None:intergenic 40.0%
!! ATATGGTGGGGATTTTCTTC+AGG + chr1_4:56015798-56015817 Msa0171310:CDS 40.0%
!! TTTCTTCAGGTTGGTGGAAA+TGG + chr1_4:56015811-56015830 Msa0171310:CDS 40.0%
GTTGAACTTCAGTTTCAGCT+TGG - chr1_4:56013879-56013898 None:intergenic 40.0%
! ATGCTGAAATTCTAAGGGCT+AGG + chr1_4:56014001-56014020 Msa0171310:CDS 40.0%
! GAAATTCTAAGGGCTAGGAA+AGG + chr1_4:56014006-56014025 Msa0171310:CDS 40.0%
TCAAGTTTCAGATACACCTG+TGG + chr1_4:56014116-56014135 Msa0171310:CDS 40.0%
AAGCAACATCATTCCTCCAA+TGG - chr1_4:56014157-56014176 None:intergenic 40.0%
TGCAATTCAGGAAGAGAAAG+AGG + chr1_4:56014330-56014349 Msa0171310:intron 40.0%
GCAATTCAGGAAGAGAAAGA+GGG + chr1_4:56014331-56014350 Msa0171310:intron 40.0%
! TTTTGCTCGACATCTGTCAT+GGG - chr1_4:56014386-56014405 None:intergenic 40.0%
! TTTTTGCTCGACATCTGTCA+TGG - chr1_4:56014387-56014406 None:intergenic 40.0%
TACGGATGCAAAGAGATCAA+AGG + chr1_4:56014467-56014486 Msa0171310:CDS 40.0%
! TTTTCATTCACTCCTAGCCT+TGG + chr1_4:56014571-56014590 Msa0171310:intron 40.0%
GGTGTATATATAGAGAGAGG+AGG + chr1_4:56014592-56014611 Msa0171310:intron 40.0%
ACAGCAGTGTTCATCATCAT+TGG - chr1_4:56014669-56014688 None:intergenic 40.0%
TTGACGGTTTGACGAAAACA+TGG + chr1_4:56014753-56014772 Msa0171310:intron 40.0%
TGACGGTTTGACGAAAACAT+GGG + chr1_4:56014754-56014773 Msa0171310:intron 40.0%
AGCCATATTGGTGAAAAGGA+GGG - chr1_4:56015459-56015478 None:intergenic 40.0%
TAGCCATATTGGTGAAAAGG+AGG - chr1_4:56015460-56015479 None:intergenic 40.0%
CGGCCTTTATTAAACATGGT+GGG + chr1_4:56015523-56015542 Msa0171310:CDS 40.0%
TGTTCACTTATGGGTCTGTA+AGG - chr1_4:56015565-56015584 None:intergenic 40.0%
! CAGGTTGGACCAAATATCAA+CGG + chr1_4:56015628-56015647 Msa0171310:CDS 40.0%
AGGTTGGACCAAATATCAAC+GGG + chr1_4:56015629-56015648 Msa0171310:CDS 40.0%
GATATCGGAAATACAGGAAG+CGG - chr1_4:56015661-56015680 None:intergenic 40.0%
!! ATAGGAAATGGTGGTAATGG+TGG - chr1_4:56015685-56015704 None:intergenic 40.0%
GTTGTGGTAATAGGAAATGG+TGG - chr1_4:56015694-56015713 None:intergenic 40.0%
! CCAACTTGAGTTGTGGTAAT+AGG - chr1_4:56015703-56015722 None:intergenic 40.0%
CCTATTACCACAACTCAAGT+TGG + chr1_4:56015700-56015719 Msa0171310:CDS 40.0%
!! ATGTGAAATGGTGGTAATGG+TGG - chr1_4:56015772-56015791 None:intergenic 40.0%
TCACATTGTACCATATGGTG+GGG + chr1_4:56015786-56015805 Msa0171310:CDS 40.0%
TGAAGAAAATCCCCACCATA+TGG - chr1_4:56015799-56015818 None:intergenic 40.0%
!! ATATGGTGGGGATTTTCTTC+AGG + chr1_4:56015798-56015817 Msa0171310:CDS 40.0%
!! TTTCTTCAGGTTGGTGGAAA+TGG + chr1_4:56015811-56015830 Msa0171310:CDS 40.0%
TTCTGCTGATGATTGCCATG+TGG + chr1_4:56013831-56013850 Msa0171310:CDS 45.0%
GGGGATGAGAAATTCAACAC+TGG - chr1_4:56013904-56013923 None:intergenic 45.0%
GATGATGAAGCAACTGCCAT+TGG + chr1_4:56014138-56014157 Msa0171310:CDS 45.0%
!! TATGATGATGGTGGTGCTAG+TGG + chr1_4:56014180-56014199 Msa0171310:CDS 45.0%
! CTAGTGGATCTTGTCCACAA+AGG + chr1_4:56014196-56014215 Msa0171310:CDS 45.0%
TTTGCTCGACATCTGTCATG+GGG - chr1_4:56014385-56014404 None:intergenic 45.0%
GGTTATTGTTGGCGCAAGTA+CGG + chr1_4:56014449-56014468 Msa0171310:CDS 45.0%
TTTGTCAATGACAGGTCCGT+GGG - chr1_4:56015329-56015348 None:intergenic 45.0%
! TTTTGTCAATGACAGGTCCG+TGG - chr1_4:56015330-56015349 None:intergenic 45.0%
TGAACCATAGGCAATGCAGA+TGG - chr1_4:56015430-56015449 None:intergenic 45.0%
ATTGGTGAAAAGGAGGGAGA+GGG - chr1_4:56015453-56015472 None:intergenic 45.0%
TATTGGTGAAAAGGAGGGAG+AGG - chr1_4:56015454-56015473 None:intergenic 45.0%
! CTCCCTCCTTTTCACCAATA+TGG + chr1_4:56015454-56015473 Msa0171310:CDS 45.0%
GTTTAATAAAGGCCGCGTCA+TGG - chr1_4:56015518-56015537 None:intergenic 45.0%
GACGCGGCCTTTATTAAACA+TGG + chr1_4:56015519-56015538 Msa0171310:CDS 45.0%
GCGGCCTTTATTAAACATGG+TGG + chr1_4:56015522-56015541 Msa0171310:CDS 45.0%
GGAATTGGCCCGTTGATATT+TGG - chr1_4:56015640-56015659 None:intergenic 45.0%
GGAAATACAGGAAGCGGAAT+TGG - chr1_4:56015655-56015674 None:intergenic 45.0%
GGTGGTGATATCGGAAATAC+AGG - chr1_4:56015667-56015686 None:intergenic 45.0%
!! GGTGGTAATGGTGGTGATAT+CGG - chr1_4:56015676-56015695 None:intergenic 45.0%
GGTGGTGATATCGGAAATAC+AGG - chr1_4:56015667-56015686 None:intergenic 45.0%
!! GGTGGTAATGGTGGTGATAT+CGG - chr1_4:56015676-56015695 None:intergenic 45.0%
TGCTCCAGTCTTTCCTTTCA+TGG + chr1_4:56015843-56015862 Msa0171310:CDS 45.0%
GCTCCAGTCTTTCCTTTCAT+GGG + chr1_4:56015844-56015863 Msa0171310:CDS 45.0%
GTACCCATGAAAGGAAAGAC+TGG - chr1_4:56015850-56015869 None:intergenic 45.0%
TTCTGCTGATGATTGCCATG+TGG + chr1_4:56013831-56013850 Msa0171310:CDS 45.0%
GGGGATGAGAAATTCAACAC+TGG - chr1_4:56013904-56013923 None:intergenic 45.0%
GATGATGAAGCAACTGCCAT+TGG + chr1_4:56014138-56014157 Msa0171310:CDS 45.0%
!! TATGATGATGGTGGTGCTAG+TGG + chr1_4:56014180-56014199 Msa0171310:CDS 45.0%
! CTAGTGGATCTTGTCCACAA+AGG + chr1_4:56014196-56014215 Msa0171310:CDS 45.0%
TTTGCTCGACATCTGTCATG+GGG - chr1_4:56014385-56014404 None:intergenic 45.0%
GGTTATTGTTGGCGCAAGTA+CGG + chr1_4:56014449-56014468 Msa0171310:CDS 45.0%
TTTGTCAATGACAGGTCCGT+GGG - chr1_4:56015329-56015348 None:intergenic 45.0%
! TTTTGTCAATGACAGGTCCG+TGG - chr1_4:56015330-56015349 None:intergenic 45.0%
TGAACCATAGGCAATGCAGA+TGG - chr1_4:56015430-56015449 None:intergenic 45.0%
ATTGGTGAAAAGGAGGGAGA+GGG - chr1_4:56015453-56015472 None:intergenic 45.0%
TATTGGTGAAAAGGAGGGAG+AGG - chr1_4:56015454-56015473 None:intergenic 45.0%
! CTCCCTCCTTTTCACCAATA+TGG + chr1_4:56015454-56015473 Msa0171310:CDS 45.0%
GTTTAATAAAGGCCGCGTCA+TGG - chr1_4:56015518-56015537 None:intergenic 45.0%
GACGCGGCCTTTATTAAACA+TGG + chr1_4:56015519-56015538 Msa0171310:CDS 45.0%
GCGGCCTTTATTAAACATGG+TGG + chr1_4:56015522-56015541 Msa0171310:CDS 45.0%
GGAATTGGCCCGTTGATATT+TGG - chr1_4:56015640-56015659 None:intergenic 45.0%
GGAAATACAGGAAGCGGAAT+TGG - chr1_4:56015655-56015674 None:intergenic 45.0%
GGTGGTGATATCGGAAATAC+AGG - chr1_4:56015667-56015686 None:intergenic 45.0%
!! GGTGGTAATGGTGGTGATAT+CGG - chr1_4:56015676-56015695 None:intergenic 45.0%
GGTGGTGATATCGGAAATAC+AGG - chr1_4:56015667-56015686 None:intergenic 45.0%
!! GGTGGTAATGGTGGTGATAT+CGG - chr1_4:56015676-56015695 None:intergenic 45.0%
TGCTCCAGTCTTTCCTTTCA+TGG + chr1_4:56015843-56015862 Msa0171310:CDS 45.0%
GCTCCAGTCTTTCCTTTCAT+GGG + chr1_4:56015844-56015863 Msa0171310:CDS 45.0%
GTACCCATGAAAGGAAAGAC+TGG - chr1_4:56015850-56015869 None:intergenic 45.0%
GATTGCCATGTGGCAACAAG+AGG + chr1_4:56013841-56013860 Msa0171310:CDS 50.0%
CATCTCCTCTTGTTGCCACA+TGG - chr1_4:56013849-56013868 None:intergenic 50.0%
! TCCTTGATCAGGTGGTGTTG+GGG - chr1_4:56013923-56013942 None:intergenic 50.0%
GTCCTTGATCAGGTGGTGTT+GGG - chr1_4:56013924-56013943 None:intergenic 50.0%
TGTCCTTGATCAGGTGGTGT+TGG - chr1_4:56013925-56013944 None:intergenic 50.0%
ACACCACCTGATCAAGGACA+TGG + chr1_4:56013925-56013944 Msa0171310:CDS 50.0%
TCTGCACCATGTCCTTGATC+AGG - chr1_4:56013934-56013953 None:intergenic 50.0%
GCAGTTGCTTCATCATCCAC+AGG - chr1_4:56014135-56014154 None:intergenic 50.0%
GATGAAGCAACTGCCATTGG+AGG + chr1_4:56014141-56014160 Msa0171310:CDS 50.0%
!! AATGCAGATGGTGGTGATGG+TGG - chr1_4:56015418-56015437 None:intergenic 50.0%
ACCACCATCTGCATTGCCTA+TGG + chr1_4:56015423-56015442 Msa0171310:CDS 50.0%
ACCATAGGCAATGCAGATGG+TGG - chr1_4:56015427-56015446 None:intergenic 50.0%
! GTTGGCCTAATACCATGACG+CGG + chr1_4:56015503-56015522 Msa0171310:CDS 50.0%
AAAGGCCGCGTCATGGTATT+AGG - chr1_4:56015511-56015530 None:intergenic 50.0%
GGGTCTGTAAGGTTGCATCA+TGG - chr1_4:56015554-56015573 None:intergenic 50.0%
! GGTGGGGATTTTCTTCAGGT+TGG + chr1_4:56015802-56015821 Msa0171310:CDS 50.0%
!!! GGGGATTTTCTTCAGGTTGG+TGG + chr1_4:56015805-56015824 Msa0171310:CDS 50.0%
AGTAGCTTCCTGATCAGCTG+GGG - chr1_4:56015875-56015894 None:intergenic 50.0%
CAGTAGCTTCCTGATCAGCT+GGG - chr1_4:56015876-56015895 None:intergenic 50.0%
TCAGTAGCTTCCTGATCAGC+TGG - chr1_4:56015877-56015896 None:intergenic 50.0%
GATTGCCATGTGGCAACAAG+AGG + chr1_4:56013841-56013860 Msa0171310:CDS 50.0%
CATCTCCTCTTGTTGCCACA+TGG - chr1_4:56013849-56013868 None:intergenic 50.0%
! TCCTTGATCAGGTGGTGTTG+GGG - chr1_4:56013923-56013942 None:intergenic 50.0%
GTCCTTGATCAGGTGGTGTT+GGG - chr1_4:56013924-56013943 None:intergenic 50.0%
TGTCCTTGATCAGGTGGTGT+TGG - chr1_4:56013925-56013944 None:intergenic 50.0%
ACACCACCTGATCAAGGACA+TGG + chr1_4:56013925-56013944 Msa0171310:CDS 50.0%
TCTGCACCATGTCCTTGATC+AGG - chr1_4:56013934-56013953 None:intergenic 50.0%
GCAGTTGCTTCATCATCCAC+AGG - chr1_4:56014135-56014154 None:intergenic 50.0%
GATGAAGCAACTGCCATTGG+AGG + chr1_4:56014141-56014160 Msa0171310:CDS 50.0%
!! AATGCAGATGGTGGTGATGG+TGG - chr1_4:56015418-56015437 None:intergenic 50.0%
ACCACCATCTGCATTGCCTA+TGG + chr1_4:56015423-56015442 Msa0171310:CDS 50.0%
ACCATAGGCAATGCAGATGG+TGG - chr1_4:56015427-56015446 None:intergenic 50.0%
! GTTGGCCTAATACCATGACG+CGG + chr1_4:56015503-56015522 Msa0171310:CDS 50.0%
AAAGGCCGCGTCATGGTATT+AGG - chr1_4:56015511-56015530 None:intergenic 50.0%
GGGTCTGTAAGGTTGCATCA+TGG - chr1_4:56015554-56015573 None:intergenic 50.0%
! GGTGGGGATTTTCTTCAGGT+TGG + chr1_4:56015802-56015821 Msa0171310:CDS 50.0%
!!! GGGGATTTTCTTCAGGTTGG+TGG + chr1_4:56015805-56015824 Msa0171310:CDS 50.0%
AGTAGCTTCCTGATCAGCTG+GGG - chr1_4:56015875-56015894 None:intergenic 50.0%
CAGTAGCTTCCTGATCAGCT+GGG - chr1_4:56015876-56015895 None:intergenic 50.0%
TCAGTAGCTTCCTGATCAGC+TGG - chr1_4:56015877-56015896 None:intergenic 50.0%
TCCCCAACACCACCTGATCA+AGG + chr1_4:56013919-56013938 Msa0171310:CDS 55.0%
GCACCATGTCCTTGATCAGG+TGG - chr1_4:56013931-56013950 None:intergenic 55.0%
ACATCTGTCATGGGGCAAGG+TGG - chr1_4:56014377-56014396 None:intergenic 55.0%
TCGACATCTGTCATGGGGCA+AGG - chr1_4:56014380-56014399 None:intergenic 55.0%
!! TGATGGTGGTGGCAGCATGT+TGG - chr1_4:56015404-56015423 None:intergenic 55.0%
! GGCAATGCAGATGGTGGTGA+TGG - chr1_4:56015421-56015440 None:intergenic 55.0%
!! GCTGGGGAAGTACCCATGAA+AGG - chr1_4:56015859-56015878 None:intergenic 55.0%
TCCCCAACACCACCTGATCA+AGG + chr1_4:56013919-56013938 Msa0171310:CDS 55.0%
GCACCATGTCCTTGATCAGG+TGG - chr1_4:56013931-56013950 None:intergenic 55.0%
ACATCTGTCATGGGGCAAGG+TGG - chr1_4:56014377-56014396 None:intergenic 55.0%
TCGACATCTGTCATGGGGCA+AGG - chr1_4:56014380-56014399 None:intergenic 55.0%
!! TGATGGTGGTGGCAGCATGT+TGG - chr1_4:56015404-56015423 None:intergenic 55.0%
! GGCAATGCAGATGGTGGTGA+TGG - chr1_4:56015421-56015440 None:intergenic 55.0%
!! GCTGGGGAAGTACCCATGAA+AGG - chr1_4:56015859-56015878 None:intergenic 55.0%
!! GCAGATGGTGGTGATGGTGG+TGG - chr1_4:56015415-56015434 None:intergenic 60.0%
GGAGGGAGAGGGTGAACCAT+AGG - chr1_4:56015442-56015461 None:intergenic 60.0%
GGGTACTTCCCCAGCTGATC+AGG + chr1_4:56015864-56015883 Msa0171310:CDS 60.0%
!! GCAGATGGTGGTGATGGTGG+TGG - chr1_4:56015415-56015434 None:intergenic 60.0%
GGAGGGAGAGGGTGAACCAT+AGG - chr1_4:56015442-56015461 None:intergenic 60.0%
GGGTACTTCCCCAGCTGATC+AGG + chr1_4:56015864-56015883 Msa0171310:CDS 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 56013801 56015915 56013801 ID=Msa0171310;Name=Msa0171310
chr1_4 mRNA 56013801 56015915 56013801 ID=Msa0171310-mRNA-1;Parent=Msa0171310;Name=Msa0171310-mRNA-1;_AED=0.03;_eAED=0.04;_QI=0|0|0|0.8|0.75|0.6|5|0|412
chr1_4 exon 56013801 56013806 56013801 ID=Msa0171310-mRNA-1:exon:16836;Parent=Msa0171310-mRNA-1
chr1_4 exon 56013829 56014075 56013829 ID=Msa0171310-mRNA-1:exon:16837;Parent=Msa0171310-mRNA-1
chr1_4 exon 56014094 56014217 56014094 ID=Msa0171310-mRNA-1:exon:16838;Parent=Msa0171310-mRNA-1
chr1_4 exon 56014340 56014507 56014340 ID=Msa0171310-mRNA-1:exon:16839;Parent=Msa0171310-mRNA-1
chr1_4 exon 56015222 56015915 56015222 ID=Msa0171310-mRNA-1:exon:16840;Parent=Msa0171310-mRNA-1
chr1_4 CDS 56013801 56013806 56013801 ID=Msa0171310-mRNA-1:cds;Parent=Msa0171310-mRNA-1
chr1_4 CDS 56013829 56014075 56013829 ID=Msa0171310-mRNA-1:cds;Parent=Msa0171310-mRNA-1
chr1_4 CDS 56014094 56014217 56014094 ID=Msa0171310-mRNA-1:cds;Parent=Msa0171310-mRNA-1
chr1_4 CDS 56014340 56014507 56014340 ID=Msa0171310-mRNA-1:cds;Parent=Msa0171310-mRNA-1
chr1_4 CDS 56015222 56015915 56015222 ID=Msa0171310-mRNA-1:cds;Parent=Msa0171310-mRNA-1
Gene Sequence

>Msa0171310

ATGTGTGCTTCTGCTGATGATTGCCATGTGGCAACAAGAGGAGATGTAAAACTCCAAGCTGAAACTGAAGTTCAACAACCAGTGTTGAATTTCTCATCCCCAACACCACCTGATCAAGGACATGGTGCAGAAGAAGAACAAGACCATGTGCAAAATGATGCAAATGTAGTGGTGAAAAATGCTGAAATTCTAAGGGCTAGGAAAGGGAAGATGCCAATGAACTTTTCTGAAGCAATGCATGCCAAAGAAGATAAAACAAATGTACCAACTGATGATCAAGTTTCAGATACACCTGTGGATGATGAAGCAACTGCCATTGGAGGAATGATGTTGCTTCAATATGATGATGGTGGTGCTAGTGGATCTTGTCCACAAAGGAAGAGAAAGAGGGACTTCTCTGAATACTATAAACCACCTTGCCCCATGACAGATGTCGAGCAAAAAATAGTGATTCGAGTTGAAAGTGATGAAGACATTTTGGATGATGGTTATTGTTGGCGCAAGTACGGATGCAAAGAGATCAAAGGAAATCCTAACAATCGTAGGGGGTACTACAGATGCGCAACACACAATTGTGACATTAAAAAACAAGTGGAAAGGGATGCTAAAGATCCAAAATATGTACTTGTTACATATGATGGAAAACATACCCACGGACCTGTCATTGACAAAAAAAGACCAACATACTCGAGAAATATTAATGCATCTGGAGTTCGTAGAAATGCCAACATGCTGCCACCACCATCACCACCATCTGCATTGCCTATGGTTCACCCTCTCCCTCCTTTTCACCAATATGGCTATATACCTTATGGTATGAATTACGGTTGGCCTAATACCATGACGCGGCCTTTATTAAACATGGTGGGTAACACCATGATGCAACCTTACAGACCCATAAGTGAACAAAATTTTCTAGAACATCGTGCTTCAACTCCTATTGATACAAATCAGGTTGGACCAAATATCAACGGGCCAATTCCGCTTCCTGTATTTCCGATATCACCACCATTACCACCATTTCCTATTACCACAACTCAAGTTGGACATAATATCAATGGGTCAGTTTCGCTTCCTGTATTTCCGATATCACCACCATTACCACCATTTCACATTGTACCATATGGTGGGGATTTTCTTCAGGTTGGTGGAAATGGTATTGCTGCTGCTCCAGTCTTTCCTTTCATGGGTACTTCCCCAGCTGATCAGGAAGCTACTGATGTTCCTGACACCGAATGA
Protein sequence

>Msa0171310

MCASADDCHVATRGDVKLQAETEVQQPVLNFSSPTPPDQGHGAEEEQDHVQNDANVVVKNAEILRARKGKMPMNFSEAMHAKEDKTNVPTDDQVSDTPVDDEATAIGGMMLLQYDDGGASGSCPQRKRKRDFSEYYKPPCPMTDVEQKIVIRVESDEDILDDGYCWRKYGCKEIKGNPNNRRGYYRCATHNCDIKKQVERDAKDPKYVLVTYDGKHTHGPVIDKKRPTYSRNINASGVRRNANMLPPPSPPSALPMVHPLPPFHQYGYIPYGMNYGWPNTMTRPLLNMVGNTMMQPYRPISEQNFLEHRASTPIDTNQVGPNINGPIPLPVFPISPPLPPFPITTTQVGHNINGSVSLPVFPISPPLPPFHIVPYGGDFLQVGGNGIAAAPVFPFMGTSPADQEATDVPDTE*