AlfalfaGEDB | Msa0172230

M. sativa cultivar zhongmu-4 / Msa0172230

KEGG: -
GO: -
PFAM: PF04504.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0172230	sp\|F4K5T4\|STKLU_ARATH	33.880	366	158	15	78	374	72	422	4.95e-42	155

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0172230	G7I7E5	92.308	377	27	2	1	377	1	375	0.0	637

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TFs/TRs:

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Gene ID	Type	Classification
Msa0172230	TF	GeBP

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0172230	MtrunA17_Chr1g0191011	92.308	377	27	2	1	377	1	375	0.0	637
Msa0172230	MtrunA17_Chr1g0191021	32.249	338	171	10	82	373	45	370	6.13e-41	148
Msa0172230	MtrunA17_Chr7g0273821	44.444	171	76	7	73	230	42	206	2.46e-28	115
Msa0172230	MtrunA17_Chr7g0273821	38.793	116	48	5	278	374	323	434	2.59e-12	67.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0172230	AT5G28040.2	33.880	366	158	15	78	374	72	422	5.03e-43	155
Msa0172230	AT5G28040.1	33.880	366	158	15	78	374	72	422	5.03e-43	155
Msa0172230	AT3G04930.1	30.105	382	174	11	78	374	78	451	2.20e-34	132
Msa0172230	AT3G04930.2	36.612	183	89	7	78	241	78	252	7.66e-26	107
Msa0172230	AT1G11510.1	35.606	132	69	4	109	230	105	230	3.59e-13	70.5
Msa0172230	AT1G11510.2	39.394	99	53	2	133	230	109	201	4.90e-13	69.7

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Find 95 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAACACAGCCACCACCG+CGG	0.714798	1_4:+57207437	Msa0172230:CDS
AACAACATCGGCGACGCACT+GGG	0.551267	1_4:-57208107	None:intergenic
AACATCCAAAACGTCATGGT+CGG	0.630878	1_4:-57207826	None:intergenic
AACATCGGCGACGCACTGGG+TGG	0.698538	1_4:-57208104	None:intergenic
AACTGGAGGTGTATTTGAAT+CGG	0.526700	1_4:+57208180	Msa0172230:CDS
AAGGAAACACAAGATGACTT+TGG	0.497734	1_4:+57207716	Msa0172230:CDS
AATCGGTTGGAGTTGTTGCA+AGG	0.547050	1_4:+57208197	Msa0172230:CDS
AATTCAGCGTGGAATTCAAT+AGG	0.439027	1_4:+57207661	Msa0172230:CDS
ACCATTGTTGAAGGAACTAT+TGG	0.296222	1_4:+57208064	Msa0172230:CDS
ACGGTGTTAAACAATATCAG+AGG	0.680774	1_4:+57207514	Msa0172230:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTCTTCTTCTTCTTCTTCTT+CGG	-	chr1_4:57207193-57207212	None:intergenic	30.0%
CATTGTATGGACATAGAAAA+TGG	-	chr1_4:57207311-57207330	None:intergenic	30.0%
TTAAACAATATCAGAGGCTA+TGG	+	chr1_4:57207520-57207539	Msa0172230:CDS	30.0%
TTCTTCTTCTTCTTCTTCTT+CGG	-	chr1_4:57207193-57207212	None:intergenic	30.0%
CATTGTATGGACATAGAAAA+TGG	-	chr1_4:57207311-57207330	None:intergenic	30.0%
TTAAACAATATCAGAGGCTA+TGG	+	chr1_4:57207520-57207539	Msa0172230:CDS	30.0%
GACAAAATCCAAGAAACCAA+TGG	-	chr1_4:57207144-57207163	None:intergenic	35.0%
TGACAATGTTGAAGACCAAA+TGG	+	chr1_4:57207260-57207279	Msa0172230:CDS	35.0%
CTGGATTACATTAAACAGCA+AGG	+	chr1_4:57207579-57207598	Msa0172230:CDS	35.0%
GCAAATACTACCATTCAAAG+TGG	+	chr1_4:57207606-57207625	Msa0172230:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	57207132	57208265	57207132	ID=Msa0172230;Name=Msa0172230
chr1_4	mRNA	57207132	57208265	57207132	ID=Msa0172230-mRNA-1;Parent=Msa0172230;Name=Msa0172230-mRNA-1;_AED=0.21;_eAED=0.21;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|377
chr1_4	exon	57207132	57208265	57207132	ID=Msa0172230-mRNA-1:exon:17479;Parent=Msa0172230-mRNA-1
chr1_4	CDS	57207132	57208265	57207132	ID=Msa0172230-mRNA-1:cds;Parent=Msa0172230-mRNA-1

Gene Sequence

>Msa0172230

ATGCTTTCACCATTGGTTTCTTGGATTTTGTCAACATCATCATCTTCCTCTTCGTCATCCGAAGAAGAAGAAGAAGAAGAAAGTCTTGACATTAACAATGACGAAGCAGACTGTGAAAACTTTGAAAATGACAATGTTGAAGACCAAATGGACAATGTGGATGATGACGAAAAGGACCATTTTCTATGTCCATACAATGTGGATGAAGACCAAATGGACAACATTCCCATTGCACTTGCCGTTTCTGATGCATCCCCCGTTGTGACGGTGGCCATGGCTGCCCCTGCAGATGACACAAACACGGCAACAAACACAGCCACCACCGCGGCTACTACCACTATAACAACACGCTCAAAACGACAATATAACAAAAAGTATTCAGACGGTGTTAAACAATATCAGAGGCTATGGACAAAGCAGGACGAGATTGAACTGTTGAAGGGATATCTGGATTACATTAAACAGCAAGGAAGAGCAAATACTACCATTCAAAGTGGCGTAGCTTCATTCTATGATCAAGTTATTCCCAAATTCAGCGTGGAATTCAATAGGAACCAGCTTGTTGAGAAGCTACGTAGGTTAAAAAGGAAACACAAGATGACTTTGGACAAAGGCAAAGAGGTTCCGATTTCTTTCAAGATCCCTCAGGAACAAGCCATTTTCGAAATTTCCCACAAGATTTGGGGCAATGACACCGACCATGACGTTTTGGATGTTGATGAATCAAGACCTGTTCCTGAAAGTCCTGATCTTATTGACAATGTTAAGATGAAGGATGAAAATGTTGACAATTGTGAAGAAACAGATAAAAGGGTGCACAAGCGTGTGAAGATAACAACAGATGACGACGTGAACACAAAAAATGACCGGAACAATGGCAATGCTACTAGCATACGGGGCTTCATTGAAGAAACCATGAAGTCTTGTTTCTCACCATTGTTGAAGGAACTATTGGATGATGTTCCAGTAGAGCCACCCAGTGCGTCGCCGATGTTGTTATCGACAGGGGAAGTGAGGGATGAACAATGGTTAAAACGGAGGATTTTGGAACTGGAGGTGTATTTGAATCGGTTGGAGTTGTTGCAAGGTCAGATCAAGGCTAGGTTGGAGGAGTTAAAGTCTAGCTTAGATTGA

Protein sequence

>Msa0172230

MLSPLVSWILSTSSSSSSSSEEEEEEESLDINNDEADCENFENDNVEDQMDNVDDDEKDHFLCPYNVDEDQMDNIPIALAVSDASPVVTVAMAAPADDTNTATNTATTAATTTITTRSKRQYNKKYSDGVKQYQRLWTKQDEIELLKGYLDYIKQQGRANTTIQSGVASFYDQVIPKFSVEFNRNQLVEKLRRLKRKHKMTLDKGKEVPISFKIPQEQAIFEISHKIWGNDTDHDVLDVDESRPVPESPDLIDNVKMKDENVDNCEETDKRVHKRVKITTDDDVNTKNDRNNGNATSIRGFIEETMKSCFSPLLKELLDDVPVEPPSASPMLLSTGEVRDEQWLKRRILELEVYLNRLELLQGQIKARLEELKSSLD*